LOCUS       NZ_LIVV01000011        18194 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02009 scaffold11, whole genome shotgun sequence.
ACCESSION   NZ_LIVV01000011
VERSION     NZ_LIVV01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02009
  ORGANISM  Streptomyces sp. CB02009
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 361851)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361851)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905825.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,214
            CDSs (total)                      :: 8,139
            Genes (coding)                    :: 7,984
            CDSs (with protein)               :: 7,984
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 51 of 155
            Pseudo Genes (incomplete)         :: 117 of 155
            Pseudo Genes (internal stop)      :: 15 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:06:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 18194
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVV01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18194
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..18194
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18194
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[7480:8194](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7481..8194
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(<1..1473)
                     /locus_tag="AMK27_RS34655"
                     /old_locus_tag="AMK27_34260"
     CDS             complement(<1..1473)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949915.1"
                     /locus_tag="AMK27_RS34655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34260"
                     /product="regulator"
                     /protein_id="WP_073917403.1"
                     /transl_table=11
                     /translation="MTERPPQRIPNRQLAALIAEAGFSHAGLARRVDQLGLEHGLDLRY
                     DKTSVTRWLRGQQPRGTTPALIAEVFTRRLGRRLSAQDLGLDACAPVYAGLEFAATPEE
                     AVDIVSGLWRKDSGSHAELRKIAFTPAGLVVPSRDWLIGRADDRVARGDAPQGPRVSSG
                     TSAVAAAVTAEGRPPHDPRPGLDPRQGLDPRLAHDPRVPPQGRFSVPRQRGTDRGPGQR
                     VSSGDIAALRSVGELFRTLDHAYGGGHARQALVRYLEHEAEPMLRGTYGESVGRRLFAA
                     AADLTRLAGWTSYDIAAHGLAQRYFVQALRLAQAAGDRAYGSYVLLTMSCQAVYLGHGR
                     EAVQLARVAQQGVGPAAPPVVQAMLHAVEARGHAVLGEARACSASLVRAERALEAARPG
                     DEVPYWARMFDEAQLADELGHCHRDLQQYRPAAQHAERALQLRAPGFARSRLFCRVVLA
                     TSRLALGELDQACALGAEAAQQASEMRSARAVE"
     gene            1811..2620
                     /gene="lipB"
                     /locus_tag="AMK27_RS34660"
                     /old_locus_tag="AMK27_34265"
     CDS             1811..2620
                     /EC_number="2.3.1.181"
                     /GO_function="GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="lipB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238896.1"
                     /locus_tag="AMK27_RS34660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34265"
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="WP_073917889.1"
                     /transl_table=11
                     /translation="MSELRFVRLGFGAEAVDYQVAWDKQREVHAARFADETDDTCLLLE
                     HPPVYTAGRRTAENERPLDGTPVVDVDRGGKITWHGPGQLVGYPIMKLPRPVDVVAHVR
                     RLEEALILTAAEFGVETSRVEGRSGVWVLGDPVEQRPALGGLSLDFDPRLNDEEFDPRL
                     NGPEYAPSNAGQRREDRKLAAIGIRVAKGVTMHGFALNVNPDNAWFDRIIPCGIRDAGV
                     TSLANELGRDVTIEEVLPVVEKHLRAVLEQADLKPREIERPVETAAV"
     misc_feature    complement(1819..1837)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            2735..3688
                     /gene="lipA"
                     /locus_tag="AMK27_RS34665"
                     /old_locus_tag="AMK27_34270"
     CDS             2735..3688
                     /EC_number="2.8.1.8"
                     /GO_function="GO:0016992 - lipoate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lipA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848859.1"
                     /locus_tag="AMK27_RS34665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34270"
                     /product="lipoyl synthase"
                     /protein_id="WP_073917404.1"
                     /sec_met_domain="PF04055 (E-value: 5e-13, bitscore: 44.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAVAPDGRKMLRLEVRNAQTPIERKPEWIKTRAKMGPEYTKMQS
                     LVKSEGLHTVCQEAGCPNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPEALDRDEP
                     RRVGESVVTMDLNYATITGVARDDLEDGGAWLYAETVRQIHQQTAERADGRTKVELLAP
                     DFNAVPELLAEVFASRPEVFAHNVETVPRIFKRIRPGFRYERSLDVITQARDYGLVTKS
                     NLILGMGETREEISEALQQLHDAGCELITITQYLRPSVRHHPVERWVKPQEFVELKEEA
                     DEIGFSGVMSGPLVRSSYRAGRLFQQAMEKRSRAGA"
     misc_feature    complement(3802..3817)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(3916..3936)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.68"
                     /tool="antismash"
     misc_feature    3946..3961
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.41"
                     /tool="antismash"
     misc_feature    3957..3972
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(3957..3974)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     gene            4073..4288
                     /locus_tag="AMK27_RS34670"
                     /old_locus_tag="AMK27_34275"
     CDS             4073..4288
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238898.1"
                     /locus_tag="AMK27_RS34670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34275"
                     /product="hypothetical protein"
                     /protein_id="WP_073917406.1"
                     /transl_table=11
                     /translation="MPAASTHVRVTAIPSVTDALKAVEALLLGAGQRTARRNAWNSVLE
                     DRRNAKDRVEAQHVMEVASGRTSRAT"
     gene            4299..5003
                     /locus_tag="AMK27_RS34675"
                     /old_locus_tag="AMK27_34280"
     CDS             4299..5003
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511895.1"
                     /locus_tag="AMK27_RS34675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34280"
                     /product="DUF4191 domain-containing protein"
                     /protein_id="WP_073917407.1"
                     /transl_table=11
                     /translation="MARKANTGSADAANQGRLKQIALTYKMTRKADPKVGLVVAGVGIV
                     VLGVLLAIGFLIGHPVYLGILGFVLALLAAAIVFGRRAERAAFGQMEGQPGAAAAVLQN
                     VGRGWTTTPAVAMNRSQDVVHRAVGRAGIVLVAEGNPNRVKPLLAAEKKRMSRVVVDVP
                     VTDIIVGDGEGQVPLKKVRTTMLKLPRVLTGPQVTAANDRLRAMGDLMSNMPLPKGPMP
                     KGMRMPRGGKMR"
     gene            complement(5132..5599)
                     /locus_tag="AMK27_RS34680"
                     /old_locus_tag="AMK27_34285"
     CDS             complement(5132..5599)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238900.1"
                     /locus_tag="AMK27_RS34680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34285"
                     /product="RDD family protein"
                     /protein_id="WP_073917409.1"
                     /transl_table=11
                     /translation="MDNRQALGSWLSGPRAAAEDAGVDFGYRGQQLGLPEEGPGSVARP
                     GRRLGALAVDWALCTLIAYGLITDGYNQATGNWALVILLVLSVLTVGTIGSTPGKRLFG
                     LGVVSVNGGRLGLPRVALRSLLVCLAIPALIWDRDGRGLHDRLSGAVQVRL"
     misc_feature    complement(5684..5701)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.63"
                     /tool="antismash"
     gene            5822..7246
                     /gene="glnA"
                     /locus_tag="AMK27_RS34685"
                     /old_locus_tag="AMK27_34290"
     CDS             5822..7246
                     /EC_number="6.3.1.2"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="glnA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033068.1"
                     /locus_tag="AMK27_RS34685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34290"
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="WP_030314285.1"
                     /transl_table=11
                     /translation="MSETHGFQNADEVKKYIKDNDVKMVDVRFCDLPGVMQHFTIPATA
                     FDPSEELAFDGSSIRGFQAIHESDMALRADLSTARVDPFRRDKTLNINFFIHDPITGEQ
                     YSRDPRNIAKKAEAYLASTGIADTAYFGPEAEFYVFDSVRFETSANQGFYHIDSEAGAW
                     NTGAEENNRGYKVRYKGGYFPAPPVDHFADLRSEISLELENVGLQVERQHHEVGTAGQA
                     EINYKFNTLLAAADDLMLFKYIVKNVAWRNGKTATFMPKPIFGDNGSGMHVHQSLWANG
                     DPLFYDETGYAGLSDTARYYIGGILKHAPSLLAFTNPTVNSYHRLVPGFEAPVNMVYSQ
                     RNRSAAMRIPITGSNPKAKRVEFRAPDPSSNPYLAFSALLLAGLDGIKNKIEPAEPIDK
                     DLYELAPEEHANVQQVPTNLEAVLNALEADNEYLQAGGVFTSDLIETWIDYKRTHEIAP
                     IQLRPHPHEFELYFDL"
     misc_feature    7380..7393
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.93"
                     /tool="antismash"
     gene            7481..8194
                     /locus_tag="AMK27_RS34690"
                     /old_locus_tag="AMK27_34295"
     CDS             7481..8194
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003999281.1"
                     /locus_tag="AMK27_RS34690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34295"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_079188683.1"
                     /sec_met_domain="CDPS (E-value: 9e-86, bitscore: 281.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQAAFAPAETLRLQPYTDHCRVIADEGAHVVIGVSPGNSYFTAQ
                     RLRELTRWGLDHFDQVDLVYTDLFVSDMYEALGYGADEARRKAVKNLRGVRAKVGAAAT
                     EADPTGVRVRARGMSEFQALPAYQELHRQVLAAVDGDPVVRATCDALTGIFLAGKLTPG
                     QEASGLQKEVCRAYISAEVPLFLDTPAILGVPSSLNCYHQALPLADLLYARGTGLRASR
                     NQGHGILTPVEGDAR"
     gene            8191..9387
                     /locus_tag="AMK27_RS34695"
                     /old_locus_tag="AMK27_34300"
     CDS             8191..9387
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033070.1"
                     /locus_tag="AMK27_RS34695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34300"
                     /product="cytochrome P450"
                     /protein_id="WP_073917412.1"
                     /sec_met_domain="p450 (E-value: 3e-14, bitscore: 46.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVMTHDLPAFPFDWDGTRLPAEVEALRAEPVRRVRTIAGAEAW
                     LVSSYELCRQVLEDPRFSLKDTSAPGVPRQYALTIPPHVVNNMGNITGAGLRKAVMKAI
                     NPKAPGLEEWLRARAGALVDALVAEGAPGELRGAYADPYSAGLHCRMLGIPEGDGPRLL
                     RSLDVAFMNAPHEIEAARLHWDRDIAYMTERLDDPATGGLMAELAALREDPEYAHLTDE
                     MLATVGVTLFGAGVISTAGFLTMALVSVLTRPDVREALTAGGAGRVAGAMDELLRVNLS
                     IGDGLPRLALEDVRLGEVEVRAGELVLVLVEAANHDPGHFPDPLVFRPDRENAADHLSF
                     GGGRHYCPATALGKRHAEIALETLLDRLPELRLAVPVEQLVWRTNFMKRLPERLPVAW"
     gene            complement(9445..9720)
                     /locus_tag="AMK27_RS34700"
                     /old_locus_tag="AMK27_34305"
     CDS             complement(9445..9720)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156638.1"
                     /locus_tag="AMK27_RS34700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34305"
                     /product="hypothetical protein"
                     /protein_id="WP_073917414.1"
                     /transl_table=11
                     /translation="MALAVIRTPSLEPWKPLRKKPLPAGRPREWYVTHNRRLKAMRLAI
                     ALLDAGVYVPSKATNATIRTTADHLGIHPPSDTTCRMVRAMIRYGR"
     misc_feature    9843..9858
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            9870..10229
                     /locus_tag="AMK27_RS34705"
                     /old_locus_tag="AMK27_34310"
     CDS             9870..10229
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885700.1"
                     /locus_tag="AMK27_RS34705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34310"
                     /product="arsenate reductase family protein"
                     /protein_id="WP_073917415.1"
                     /transl_table=11
                     /translation="MEIWINPACSKCRSALTLLNAEGADYTVRRYLEDVPSPDEIRQVL
                     DRLGLEPWDITRTREAVAKELGLKEWARDEGSRERWIEALSAHPELIQRPIITAEDGTA
                     VVGRSEEAVRDALSR"
     misc_feature    complement(9873..9886)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(10226..11176)
                     /locus_tag="AMK27_RS34710"
                     /old_locus_tag="AMK27_34315"
     CDS             complement(10226..11176)
                     /NRPS_PKS="Domain: Abhydrolase_1 (68-169). E-value:
                     4.3e-18. Score: 57.8. Matches aSDomain:
                     nrpspksdomains_AMK27_RS34710_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033073.1"
                     /locus_tag="AMK27_RS34710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34315"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_198955989.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-15, bitscore: 51.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 5e-19, bitscore:
                     64.1, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTPRTSDNDSAHDSTTDLVHASASASASAFGSAYDDLLATWPHP
                     TTTSELPTPYGTTHLLHHGAEDGPPVLLLPGGGSTAGSWRTTAAALGTTHRVHAVDLVG
                     GPGRSGTDGRPIRTAADLTAWLDAVLDGLGLASAAFCGHSYGGWIALRYALAAPDRTDR
                     LVLLDPTGCFAGFRPGYLLRALPTLLRPTAARTAAFLAWETGGADHDPRVRRLDTLAAE
                     LPRLRPVTGPRPTATALAALRPPALVLLAEGSRTHDAREAARTATALGLRTELVPGATH
                     HTLPATLPPKALTALTDFLATGGQRPPGLAGERSP"
     aSDomain        complement(10670..10972)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK27_RS34710_Abhydrolase_1.1"
                     /evalue="4.30E-18"
                     /label="AMK27_RS34710_Abhydrolase_1.1"
                     /locus_tag="AMK27_RS34710"
                     /protein_end="169"
                     /protein_start="68"
                     /score="57.8"
                     /tool="antismash"
                     /translation="PPVLLLPGGGSTAGSWRTTAAALGTTHRVHAVDLVGGPGRSGTDG
                     RPIRTAADLTAWLDAVLDGLGLASAAFCGHSYGGWIALRYALAAPDRTDRLVLLDP"
     CDS_motif       complement(10724..10756)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AMK27_RS34710_0001"
                     /evalue="2.90E-03"
                     /label="NRPS-te1"
                     /locus_tag="AMK27_RS34710"
                     /protein_end="151"
                     /protein_start="140"
                     /score="10.2"
                     /tool="antismash"
                     /translation="CGHSYGGWIAL"
     gene            11251..11685
                     /locus_tag="AMK27_RS34715"
                     /old_locus_tag="AMK27_34320"
     CDS             11251..11685
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087434.1"
                     /locus_tag="AMK27_RS34715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34320"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_073917893.1"
                     /transl_table=11
                     /translation="MLIFHLLREVTVEYGLRQGEFAARNGMHPTDVRALICLLDAERAG
                     TDATAGWLGERLGLNSAGTTAVVDRLERLGHVARVRDARDRRRVLLTVEDRAKELGRAH
                     FGPLVAGAVALLGGFEEGEREAVRRFLTGVRDLLSDGEKA"
     gene            11682..13196
                     /locus_tag="AMK27_RS34720"
                     /old_locus_tag="AMK27_34325"
     CDS             11682..13196
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (123-408). E-value:
                     8.3e-18. Score: 56.5. Matches aSDomain:
                     nrpspksdomains_AMK27_RS34720_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123983.1"
                     /locus_tag="AMK27_RS34720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34325"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_073917417.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.4e-17, bitscore:
                     56.6, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAADHDGLGVDLHLELSAAGGRRAVLIAALREAVRGGRLAPGTR
                     LPPYRSLAADLGIARNTVADAYAELVAEGWLTSRQGSGTRVADRVAAPAPRPVRTTAPP
                     PLPYDLVQGKPDPGAFPRAAWLAAARRALAAAPHEAFGIGDPHGRIELRLALAEYLARV
                     RGVRTAPDRIVVCSGAAHALRLLARVLGPAAPWAVEAYGLPFHRGLLAEAGVTTLPVAV
                     DGDGARVAEIPEHARAVLLTPAHQFPTGGPLHPERRAGVLEWARASGGLVIEDDYDGEF
                     RYDRRPVGAVQGLDPERTALIGSVSKSLSPALRIGWMCLPSALVEPVVAAKGRREPYAS
                     ATDQLTLADLLATGAYDRHVRRMRQRHRARRDRLTAVLAERVPAVRVTGIAAGLHAVLE
                     LPAGTERAALAATARRGVAVEGLAPYRHPGARDATGPDDPDGPDDPAARDGSAPRDGSA
                     PRDGSAPPVAPGPPDGLVVGYATPPERLYPGALDALCAGLSEALGS"
     aSDomain        12051..12905
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK27_RS34720_Aminotran_1_2.1"
                     /evalue="8.30E-18"
                     /label="AMK27_RS34720_Aminotran_1_2.1"
                     /locus_tag="AMK27_RS34720"
                     /protein_end="408"
                     /protein_start="123"
                     /score="56.5"
                     /tool="antismash"
                     /translation="AWLAAARRALAAAPHEAFGIGDPHGRIELRLALAEYLARVRGVRT
                     APDRIVVCSGAAHALRLLARVLGPAAPWAVEAYGLPFHRGLLAEAGVTTLPVAVDGDGA
                     RVAEIPEHARAVLLTPAHQFPTGGPLHPERRAGVLEWARASGGLVIEDDYDGEFRYDRR
                     PVGAVQGLDPERTALIGSVSKSLSPALRIGWMCLPSALVEPVVAAKGRREPYASATDQL
                     TLADLLATGAYDRHVRRMRQRHRARRDRLTAVLAERVPAVRVTGIAAGLHAVLELPAGT
                     ERAA"
     gene            complement(13232..14137)
                     /locus_tag="AMK27_RS34725"
                     /old_locus_tag="AMK27_34330"
     CDS             complement(13232..14137)
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033076.1"
                     /locus_tag="AMK27_RS34725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34330"
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="WP_073917419.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 1.4e-22, bitscore:
                     75.0, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 1.7e-08,
                     bitscore: 28.9, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKQLGAKPRIGLLGTGPWAGHTHAPALAGHPGVEFSGVWGRRPE
                     AAEALATASGTRAYDDVDALFEASDAVALALPPDVQAPLAVRAARAGCHVLLDKPVATT
                     VAGAREVAEAVEAAGAASVVFCTLRFAEPTARWIDEQTAAGGWFLGEAHWLGSLYGTGS
                     ASAYAASPWRREKGGLWDVGPHVLSVFLPVLGDVTEITAARGPADTHHLVLRHTSGASS
                     TATLTLSAPPEAAEAALTLYGTGGRAEMPRWDGAVGAFGAAVDALVDAARTGEPDACDV
                     RFGLRLTEILTTAESKVAAA"
     misc_feature    complement(14235..14246)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.02"
                     /tool="antismash"
     gene            14362..15771
                     /locus_tag="AMK27_RS34730"
                     /old_locus_tag="AMK27_34335"
     CDS             14362..15771
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885710.1"
                     /locus_tag="AMK27_RS34730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34335"
                     /product="hypothetical protein"
                     /protein_id="WP_107425744.1"
                     /transl_table=11
                     /translation="MSELVAAEDVLLFVNAAVTATGQREFRSSAAEQRFSLRFVHEYVR
                     VNYRPVYAAALALDINHHNAALVVEQLLRTADEAGGPEEKRAEGRLIAARLALLPPQRV
                     YRLFRTLRAAGVNNRRTRAIVRGWLAARPDPALDAVKYRSGLKASLRHVHGRLPDPETG
                     DFLFAPGRRVRYENPTLDAFRRARYEQGALYELPFTVAEGFAAAHGVPRAVFLERIAPR
                     MTRLEQLRTERAADLSVMPLTRLALYVLSLPFGERVERRAELTGALRAAGRRAAGPYAG
                     SWGRVTAVLDDSFSSSGSAVKRRRPLAVALGSHHLLEALAAPGAYTPLWTSGGDDPLLV
                     RPYGATPLGLRVLDGLETGPDRLVIVTDGWDNAPPGLAGEVLRVWRSRLDPERRTSVVH
                     LNPVYDAQGFDVRRLAPSVPAAGIRDAEDLAALVEIAQFAEGRTGFAELRAYLDRRVEL
                     FLKAAEEGGRG"
     gene            15768..16988
                     /locus_tag="AMK27_RS34735"
                     /old_locus_tag="AMK27_34340"
     CDS             15768..16988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033078.1"
                     /locus_tag="AMK27_RS34735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34340"
                     /product="hypothetical protein"
                     /protein_id="WP_073917422.1"
                     /transl_table=11
                     /translation="MTRIDLTGLDVRPAQVWGGVRLVPLVRSEPVPGLRLHREVYGGFG
                     SVDLPDRSTYTSYVPHAFVADWTGAGSGAGPAPGAGRESAAYGTRLGGGGGGRGDGGRD
                     EAPDCVPVRRVHRMAKRRRDKDGRAEARLRFLPLHLALEGYLALHFGGPSIVWDEWSRE
                     ALRHGLSPRAEAAYAGWAVPGLTEALRIFEVHPGQCGLLLYVADSLAAAFVVPHPDDYR
                     LLHPTLVEDLYGELVHQYAYYGGPVPEFTARIRDGAGGIRTLADLRAAARQQERAWAAA
                     HDGLMARDLLETPYAFERVHRAGPFDLHRFLPPFERGGREQHIGELITDRKGRTAYLKT
                     FRLSENQIRKGYLLRRLADCDWHLGRAAEALGTSYTEVVRRVRSAGLGAFLDEHSVARR
                     LREDRES"
     misc_feature    complement(16989..17006)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.72"
                     /tool="antismash"
     gene            17127..18158
                     /gene="glnII"
                     /locus_tag="AMK27_RS34740"
                     /old_locus_tag="AMK27_34345"
     CDS             17127..18158
                     /EC_number="6.3.1.2"
                     /codon_start=1
                     /gene="glnII"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989747.1"
                     /locus_tag="AMK27_RS34740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_34345"
                     /product="glutamine synthetase"
                     /protein_id="WP_073917423.1"
                     /transl_table=11
                     /translation="MTFKAEYIWIDGTEPTAKLRSKTKIIVGGGATLGELPIWGFDGSS
                     TNQAKGHASDRVLKPVFVCPDPIRGGDDVLVLCEVLNTDMTPHESNTRAALAEVAAKYA
                     AQEPIFGIEQEYTFFEGTRPLGFPVNGFPAAQGGYYCGVGADEIFGRPIVEAHLENCLK
                     AGLGISGINAEVMPGQWEFQVGPLAPLEVSDQLWIARWLLYRTAEDFNVSATLDPKPVK
                     GDWNGAGAHTNFSTKAMREGYDAIITAAESLGEGSKPLDHVKNYGAGIDDRLTGLHETA
                     PWDQYSYGVSDRGASVRIPWQVEQDQKGYIEDRRPNANVDPYTVTRLLVDTCCEALAKA
                     GQV"
ORIGIN
        1 ctccaccgcc cgcgccgacc gcatctccga ggcctgctgc gcggcctccg caccgagcgc
       61 gcacgcctgg tccagttcgc ccagggcgag ccgtgacgtg gccaggacca cccggcagaa
      121 gaggcggctg cgggcgaagc cgggggcccg gagctggagg gcgcgttccg cgtgctgggc
      181 ggccggccgg tactgctgga ggtcgcggtg gcagtgcccg agctcgtcgg cgagctgggc
      241 ctcgtcgaac atccgggccc agtacggcac ctcgtcaccc ggccgtgccg cctccagggc
      301 ccgctcggcc cgtacgagcg aggcgctgca cgcccgtgcc tcgccgagca ccgcgtgccc
      361 ccgcgcctcg accgcgtgga gcatcgcctg gacgaccggc ggcgcggccg gcccgacgcc
      421 ctgctgggcg acccgggcga gctggacggc ctcccggccg tggccgaggt agacggcctg
      481 gcagctcatg gtgaggagca cgtacgagcc gtacgcccgg tccccggcgg cctgggcgag
      541 ccgcagtgcc tggacgaagt agcgctgggc gaggccgtgc gcggcgatgt cgtacgaggt
      601 ccagccggcg agccgggtga ggtcggcggc cgcggcgaag agccgccggc cgacggactc
      661 gccgtacgtg ccgcgcagca tcggctcggc ctcgtgttcc aggtagcgga cgagggcctg
      721 ccgggcgtgc ccgccgccgt aggcgtggtc gagcgtgcgg aagagctcgc cgaccgagcg
      781 cagcgccgcg atgtccccgc tggagacccg ctggccgggc ccccggtcgg tgccgcgctg
      841 ccggggcacc gagaagcggc cctggggcgg caccctgggg tcgtgcgcga gccgggggtc
      901 gaggccctga cgcgggtcga ggcccgggcg cgggtcgtgc ggcgggcggc cctcggccgt
      961 gaccgcggcg gcgacggccg aggtgccgga cgacacccgg gggccctgcg gggcgtcgcc
     1021 ccgcgccacc cggtcgtccg cgcggccgat cagccagtcg cggctgggta cgaccaggcc
     1081 cgccggggtg aaggcgatct tgcggagctc ggcgtggctg ccggagtcct tccgccagag
     1141 cccgctgacg atgtcgaccg cctcctcggg ggtcgcggcg aactccaggc ccgcgtagac
     1201 cggggcgcag gcgtcgaggc cgaggtcctg ggcggacagc cggcggccga ggcgacgggt
     1261 gaagacctcg gcgatcagcg cgggagtggt gccgcgcggc tgctggccgc ggagccagcg
     1321 ggtcaccgag gtcttgtcgt accgcaggtc gagaccgtgt tccagaccga gctggtccac
     1381 ccgtctcgcc aggcccgcat gggagaaccc cgcttcggcg atgagcgcgg cgagctggcg
     1441 gttcgggatg cgctgcggtg gtcgttccgt catcagctgt gcggtctcct gccttccggg
     1501 tcccgccggc agccccgtcg gaccctcatg gaacggcgtg aatttagcgg tactcacggc
     1561 ctgtaccgcc gccttcgccc cacattcatc cgatcgtgtg aggattgacg gcatcgctga
     1621 cgtaggcgct ctcggccatc cgccggagga gctcccgccc ggcgggccag gacgcggtcc
     1681 ccgtccacct gccggagggc cgttcccggc accgccgtac agtggcatgg gcgcgaatga
     1741 cgcatggtgc cgcaccggcc cgggatgatc cccggattcc ggtccccggc acccaggagg
     1801 aggcatggcc gtgagtgagc tgcgattcgt ccgccttggg ttcggcgcgg aagccgtcga
     1861 ctaccaggtg gcctgggaca agcagcgcga ggtgcacgcc gcgcggttcg ccgacgagac
     1921 cgacgacacc tgtctgctcc tcgagcaccc gccggtctac acggccggcc ggcgtacggc
     1981 ggagaacgag cgcccgctcg acgggacgcc cgtggtggac gtcgaccgcg gcggcaagat
     2041 cacctggcac ggccccggtc agctcgtcgg ctacccgatc atgaagctgc cccgcccggt
     2101 ggacgtcgtc gcacatgtgc gccggctgga ggaggcgctg atcctcaccg ccgccgagtt
     2161 cggcgtggag accagccggg tcgagggacg cagcggcgtc tgggtgctcg gcgacccggt
     2221 cgagcagcgc ccggcgctcg gcgggctctc gctcgacttc gacccgcggc tgaacgacga
     2281 ggagttcgac ccgcgtctga acggccccga gtacgccccg tccaacgcgg gccagcgccg
     2341 cgaggaccgc aagctcgccg cgatcggcat ccgggtcgcc aagggcgtca cgatgcacgg
     2401 cttcgccctc aacgtgaacc cggacaacgc gtggttcgac cggatcatcc cgtgcggcat
     2461 ccgcgacgcg ggggtcacct cgctcgccaa cgagctgggc cgcgacgtca cgatcgagga
     2521 ggtgctgccg gtggtggaga agcacctgcg ggcggtcctg gagcaggcgg acctgaagcc
     2581 gcgcgagatc gagcgtccgg tggagacggc cgcggtctga ggtgtgcccg caggaatgca
     2641 gggcgctggc cagaggttgg ccagacgtag taaggcatgg gaagcaacgg cgtaccctgg
     2701 tgtgcgccga agaatcgaag ctacaggagc cgatgtgtcc gcagtcgcac ccgacggacg
     2761 caagatgctg cgcctggagg tccggaacgc ccagaccccc atcgagcgca agcccgagtg
     2821 gatcaagacg cgggcgaaga tggggcccga gtacacgaag atgcagagcc tcgtgaagag
     2881 cgaggggctg cacacggtct gccaggaggc gggctgcccc aacatcttcg agtgctggga
     2941 ggaccgcgag gcgaccttcc tcatcggcgg tgaccagtgc acccggcgct gcgacttctg
     3001 ccagatcgac acgggcaagc ccgaggcgct ggaccgggac gagccgcgcc gtgtgggcga
     3061 gtccgtcgtc acgatggatc tgaactacgc caccatcacc ggcgtcgcgc gcgacgacct
     3121 ggaggacggc ggcgcctggc tgtacgcgga gaccgtgcgc cagatccacc agcagaccgc
     3181 ggagcgcgcc gacggccgca ccaaggtcga gctgctcgcg cccgacttca acgcggtgcc
     3241 ggagctcctg gcggaggtct tcgcctcccg cccggaggtc ttcgcgcaca acgtggagac
     3301 cgtgccgcgg atcttcaagc ggatccgtcc gggattccgt tacgagcgtt cgctcgacgt
     3361 catcacgcag gcccgtgact acggtctggt cacgaagtcg aacctgatcc tcggcatggg
     3421 cgagacccgt gaggagatca gcgaggcgct ccagcagctg cacgacgcgg gctgcgagct
     3481 gatcacgatc acgcagtacc tgcgcccctc cgtccggcac cacccggtgg agcgctgggt
     3541 gaagccgcag gagttcgtgg agctcaagga ggaggccgac gagatcggct tctccggtgt
     3601 catgtcgggc ccgctggtcc ggtcctcgta ccgcgcgggt cgcctcttcc agcaggcgat
     3661 ggagaagcgc agccgagccg gagcgtaggt tgcgggtccg cccgaggtac gagggcgggc
     3721 acgcggccgg agcggtggcg aggcggagct cgaccggcag cacggagaag cgcggcgcgg
     3781 tggacaacgc cacgcaggct gtgtgaatcg gagcacaagc ggttaccggt cagtaatggc
     3841 cggatcggac gcggcccgta cggccccgca gctcagcggg gatgtacggg ccgcgtccgc
     3901 gtttcccgtg ccctccacat ggccgcatca acgtttcatc agtgtttgac caccgggtca
     3961 cgccctggta acaccagtct gtgagcctgg tttcactcac cgccacgact cgctcacatc
     4021 cccgctcgta ccccccgcct cgtctcatcc gcaatcgagg gaggaccccg acatgccggc
     4081 cgcatcgacg cacgtccgcg tcaccgcgat cccgtccgtc accgacgccc tgaaggccgt
     4141 ggaggcgctg ctcctcggcg ccgggcagcg caccgcccgg cgcaacgcct ggaactccgt
     4201 cctggaggac cggcgcaacg ccaaggaccg ggtcgaggcc cagcacgtga tggaggtcgc
     4261 gtcgggccgg acgtcccggg ccacgtaaac ttcaggacat ggcgaggaag gcaaacacgg
     4321 gcagcgctga cgctgcgaac caggggcggc tcaagcagat cgccctcacg tacaagatga
     4381 cccgaaaggc tgaccccaag gtcggtcttg tcgtcgcagg cgtgggcatc gtcgtactcg
     4441 gtgtcctcct cgcgatcggg ttcctgatcg gtcacccggt ctacctgggc atcctgggct
     4501 tcgtcctggc gctcctggcg gcggccatcg tgttcgggcg gcgcgccgag cgcgcggcgt
     4561 tcgggcagat ggagggccag ccgggcgctg ccgcggcggt gctccagaac gtcgggcgcg
     4621 gctggacgac gacccccgcg gtcgcgatga accgcagcca ggacgtcgtg caccgggcgg
     4681 tcggccgggc gggcatcgtg ctggtggcgg agggcaaccc gaaccgggtg aagcccctgc
     4741 tggcggccga gaagaagcgc atgagccgcg tcgtggtgga cgtgccggtg accgacatca
     4801 tcgtcggcga cggcgagggc caggtgccgc tcaagaaggt ccgtacgacc atgctcaagc
     4861 ttcctcgggt gctcaccggc ccgcaggtga cggcggccaa cgaccggctg cgggcgatgg
     4921 gcgacctgat gagcaacatg ccgctgccga agggtccgat gccgaagggc atgcggatgc
     4981 cgcggggcgg aaagatgcgc tgacgcgccc gaccggaggc gacggccttc agaactgctg
     5041 catgaggaag gccccggacc gtcacacggt ccggggcctt cctcatggga tctgcgccga
     5101 cgaggctcct cggccgccgg cggcgcaggt gtcagaggcg gacctgtacg gcgccggaga
     5161 ggcggtcgtg gaggccgcgg ccgtcgcggt cccagatgag ggcggggatc gccaggcaga
     5221 cgagcaggga gcggagcgcc acccgcggga ggccgaggcg gccgccgttc acggagacga
     5281 ccccgaggcc gaagaggcgc ttgccggggg tggagccgat ggtgccgacc gtcagcacgc
     5341 tcaggacgag caggatgacg agggcccagt tgccggtggc ctggttgtag ccgtcggtga
     5401 tcaggccgta tgcgatcagc gtgcagaggg cccagtcgac ggcgagggcg ccgagacggc
     5461 gcccggggcg ggcgaccgaa ccaggcccct cctcgggcag ccccagctgc tgaccgcggt
     5521 agccgaagtc gacgcctgcg tcctcggccg ccgcgcgggg gccggagagc caagatccga
     5581 gtgcttgcct gttgtccacc cgaccacggt actgcgcccg tttttgatca gttcgggccg
     5641 ggtaggcgaa gggtcccgac cggcgcggcg gcacccacct cggttaactt cgacgaaaca
     5701 attgggtcat gcttgagaaa tcccgtctgc ctatggtcgg gtccagcgtg tgccaccgca
     5761 ctggccgcac gaaccagctg caaaccccgc ccctccccgg gccgggagta ggaggagtgg
     5821 gatgtccgag acgcacgggt tccagaacgc cgacgaggtc aagaagtaca tcaaggacaa
     5881 cgacgtcaag atggtcgacg tccgtttctg cgaccttccg ggcgtgatgc agcacttcac
     5941 gatcccggcg acggcgttcg acccgtcgga ggagctcgcc ttcgacggtt cgtcgatccg
     6001 cggcttccag gcgatccacg agtccgacat ggcgctccgc gccgacctgt cgacggcccg
     6061 tgtggacccc ttccgccgcg acaagacgct gaacatcaac ttcttcatcc acgacccgat
     6121 cacgggcgag cagtacagcc gtgacccgcg gaacatcgcg aagaaggccg aggcgtacct
     6181 cgcgtccacc ggcatcgccg acaccgcgta cttcggcccc gaggccgagt tctacgtgtt
     6241 cgactcggtg cgcttcgaga cctccgcgaa ccagggcttc taccacatcg actccgaggc
     6301 cggcgcctgg aacaccggtg cggaggagaa caaccgcggc tacaaggtcc gctacaaggg
     6361 cggctacttc ccggccccgc cggtcgacca cttcgccgac ctgcgctcgg agatctccct
     6421 ggagctggag aacgtcggcc tccaggtcga gcgccagcac cacgaggtcg gcaccgccgg
     6481 ccaggccgag atcaactaca agttcaacac gctgctcgcc gcggccgacg acctgatgct
     6541 cttcaagtac atcgtgaaga acgtcgcctg gcgcaacggc aagaccgcga ccttcatgcc
     6601 gaagccgatc ttcggcgaca acggctcggg catgcacgtc caccagtcgc tgtgggcgaa
     6661 cggcgacccg ctgttctacg acgagacggg ctacgcgggc ctctcggaca ccgcccgcta
     6721 ctacatcggc ggcatcctca agcacgcccc gtcgctgctg gccttcacca acccgacggt
     6781 gaactcgtac caccgcctgg tcccgggctt cgaggccccg gtcaacatgg tgtactcgca
     6841 gcgcaaccgc tccgccgcca tgcgcatccc gatcacgggc tcgaacccga aggccaagcg
     6901 cgtcgagttc cgcgcgccgg acccgtcctc gaacccgtac ctcgccttct cggcgctgct
     6961 gctcgcgggc ctcgacggca tcaagaacaa gatcgagccg gcggagccga tcgacaagga
     7021 cctctacgag ctcgctcccg aggagcacgc gaacgtccag caggtcccga ccaacctgga
     7081 ggccgtgctc aacgccctcg aggcggacaa cgagtacctg caggccggcg gcgtcttcac
     7141 gtccgacctg atcgagacgt ggatcgacta caagcgcacc cacgagatcg ccccgatcca
     7201 gctgcgcccg cacccgcacg agttcgagct ctatttcgac ctctaagccg taccgcgcgc
     7261 gaggttccgc cgaggcccgt cgcccctttc gagggggcgg cgggcctcgc cgttttccgg
     7321 ccaagatcac aggagtgcga acaggtgacg aactctcgtc cgtacctacg agtagggggt
     7381 tcacgctccg cgaccggtcg atagcgtgag gtcgtcgccg ccgccacacc cggagcactg
     7441 ggctccgctg ggactagcgg gcgcgatgag gactgccgac gtgactcagg cggctttcgc
     7501 ccctgcagag acgctccgac tccagccgta caccgatcac tgccgcgtca tcgccgacga
     7561 gggcgcgcac gtggtcatcg gcgtctctcc gggaaacagt tatttcaccg cccagcggct
     7621 gcgcgagctg acgcgctggg gcttggacca cttcgatcag gtcgatctcg tctacaccga
     7681 cctgttcgtc tccgacatgt acgaggcgct gggatacggg gccgacgagg cgcggcgcaa
     7741 ggccgtcaag aacctgcggg gggtgcgggc gaaggtcggg gccgccgcga ccgaggccga
     7801 tcccacgggg gtacgggtac gggcccgcgg gatgtcggag ttccaggccc tccccgccta
     7861 ccaggagctg caccggcagg tgctggcggc cgtcgacggg gatccggtcg tacgggcgac
     7921 ctgcgacgcg ctcaccggga tcttcctggc cgggaagctg acgccggggc aggaggcgag
     7981 cgggctccag aaggaggtgt gccgggcgta catcagcgcc gaggtgccgc tgttcctgga
     8041 caccccggcg atcctcgggg tgccgtcctc gctcaactgc taccaccagg ccctgcccct
     8101 ggccgatctg ctctacgccc ggggcaccgg gctgcgtgcc tcccgcaacc aggggcacgg
     8161 catcctcacg cctgtcgaag gagacgcgcg atgaccgcgg tcatgacgca cgacctgccc
     8221 gcgttcccct tcgactggga cgggacccgg ctgcccgccg aggtcgaggc gctccgcgcc
     8281 gaacccgtcc ggcgggtgcg gacgatcgcc ggggccgagg cctggctggt ctcctcgtac
     8341 gagctgtgcc ggcaggtcct ggaggacccg cggttcagcc tcaaggacac ctctgcgccg
     8401 ggggtgccgc ggcagtacgc gctgacgatc ccgccgcacg tggtgaacaa catgggcaac
     8461 atcaccggcg ccgggctgcg caaggccgtc atgaaggcga tcaacccgaa ggcgccgggg
     8521 ctcgaggagt ggctgcgggc gcgggccggg gccctggtgg acgcgctggt cgccgagggg
     8581 gcgcccgggg agctgcgggg cgcctacgcc gatccgtact cggcggggct gcactgccgg
     8641 atgctgggca tcccggaggg ggacgggccg cggctgctgc gcagtctgga cgtggccttc
     8701 atgaacgccc cgcacgagat cgaggcggcc cggctccact gggaccggga catcgcgtac
     8761 atgaccgagc gcctggacga tccggcgacg ggcgggctga tggcggagct cgcggcgctg
     8821 cgcgaggatc ccgagtacgc gcacctgacg gacgagatgc tggcgacggt gggcgtgacg
     8881 ctgttcgggg ccggggtgat ctccaccgcc gggttcctga cgatggcgct cgtgtccgtg
     8941 ctgacccggc cggacgtgcg ggaggcgctg accgcgggcg gcgcgggccg ggtcgccggg
     9001 gcgatggacg aactgctgcg ggtgaacctg tcgatcggcg acgggctgcc gcggctggcc
     9061 ctggaggacg tacggctcgg cgaggtcgag gtgcgggccg gtgaactggt cctggtgctg
     9121 gtggaggccg cgaaccacga cccggggcac ttccccgacc cgctggtctt ccggccggac
     9181 cgggagaacg ccgccgacca cctctccttc ggcggtggac ggcactactg cccggcgacg
     9241 gcgctgggca agcggcacgc cgagatcgcc ctggagacgc tgctcgaccg gctgccggag
     9301 ctgcggctcg cggtgccggt cgaacagctg gtgtggcgca ccaacttcat gaagcggctc
     9361 cccgaacggc tgccggtggc ctggtagcgg gcggggtccg acgacggcgc tgcccggccg
     9421 gggccgggca gcgcccctcc gccgtcagcg gccgtagcgg atcatggcgc gcaccatgcg
     9481 gcaggtggtg tccgagggcg ggtggatgcc gaggtggtcg gccgtggtgc gtatcgtcgc
     9541 gttcgtggcc ttcgacggga cgtagacgcc ggcgtcgagg agggcgatcg cgaggcgcat
     9601 ggccttcagt cggcggttgt gcgtcacgta ccactcgcgg gggcggcccg cggggagcgg
     9661 cttcttccgg agcggcttcc agggttcgag ggacggtgtc cggatcacgg cgagagccat
     9721 gggctacctc ctggcgaacg gcggggtgcg ggccctcccc gggaccctca ccttcacccc
     9781 ttattttagt gccccccact gacaatcgcc cctggccaga gggggtttgg ggggtggtgg
     9841 agagtgccct ggggggtacc gtgggagaca tggagatctg gatcaatccc gcctgttcca
     9901 agtgccgcag cgccctcacc cttctcaacg cggagggcgc cgactacacc gtgcgccgct
     9961 acctggagga cgtgccctcc ccggacgaga tccggcaggt gctcgaccgg ctcgggctcg
    10021 aaccgtggga catcacgcgg acgcgggagg ccgtggccaa ggagctcggg ctgaaggagt
    10081 gggcccgcga cgaagggagc cgggagcggt ggatcgaggc gctctccgcg caccccgagc
    10141 tcatccagcg gccgatcatc acggcggagg acggcacggc cgtcgtcggc cggtccgagg
    10201 aggccgtccg ggacgcgctc tcccgttagg gcgagcgttc tcccgccagg ccgggcgggc
    10261 gttgcccccc ggtcgccagg aagtccgtga gagcggtcag ggccttcggc ggcagggtcg
    10321 ccgggagggt gtggtgggtg gctcccggga cgagttccgt ccgcaggccc agcgccgtcg
    10381 ccgtcctcgc cgcctcgcgg gcgtcgtgcg tccggctgcc ctccgccagg aggaccaggg
    10441 ccggcgggcg gagggccgcg agtgcggtgg cggtcgggcg ggggcccgtg accgggcgga
    10501 ggcgcgggag ttccgccgcc agggtgtcca ggcggcgtac ccgggggtcg tggtccgcgc
    10561 cgcccgtctc ccacgccagg aacgccgccg tccgcgcggc cgtcggacgc agcagcgtgg
    10621 gcagcgcccg cagcaggtag ccggggcgga atccggcgaa gcagccggtc gggtcgagca
    10681 gcacgagacg gtcggtccgg tccggggccg ccagggcgta ccggagcgcg atccacccgc
    10741 cgtaggagtg gccgcagaag gccgccgagg ccagtccgag gccgtcgagc acggcgtcca
    10801 gccaggccgt cagatcggcg gccgtgcgga tcgggcggcc gtccgtcccg ctgcggcccg
    10861 ggccgccgac caggtccacc gcgtgcaccc ggtgcgtggt gccgagcgcg gcggccgtgg
    10921 tgcgccagga gccggcggtc gacccgccgc ccggcaggag gaggaccggc gggccgtcct
    10981 cggcgccgtg gtgcaggagg tgcgtggtgc cgtagggggt ggggagttcc gaggtcgtcg
    11041 tggggtgcgg ccaggtcgcg agcaggtcgt cgtacgcgga gccgaaggcg gaggccgagg
    11101 cggaggccga ggcgtggacc aggtccgtgg tggagtcgtg ggcggagtcg ttgtcggagg
    11161 ttcggggcgt gggcatctcc tactcctctc gttcggcgat actctcgctc agcgagataa
    11221 taggaggacg cggtggccga gtcacccgag atgctcatct ttcatctgct gcgcgaggtc
    11281 accgtcgaat acggcctccg gcagggcgag ttcgccgccc gcaacggcat gcaccccacc
    11341 gacgtacggg ccctgatctg cctcctcgac gccgagcgcg cgggcaccga cgccaccgcc
    11401 ggatggctgg gcgagcggct cggactcaac tcggccggca cgaccgccgt cgtcgaccgg
    11461 ctcgaacgcc tcggccacgt cgcccgcgtc cgtgacgccc gcgaccgtcg gcgcgtgctc
    11521 ctcaccgtgg aggaccgggc caaggaactg gggcgtgccc acttcgggcc cctcgtcgca
    11581 ggggccgtcg cgctgctcgg cgggttcgag gagggcgaga gggaggccgt acggcggttc
    11641 ctgaccggcg tacgggacct cctgagcgac ggtgagaagg cgtgaccgcc gcggaccacg
    11701 acgggctcgg ggtcgatctg catctggagc tgagcgccgc cggcgggcgg cgggccgtgc
    11761 tcatcgccgc cctgcgcgag gccgtacgcg gcgggcggct cgccccgggg acccggctgc
    11821 cgccctaccg gtcgctcgcc gccgacctcg gcatcgcccg gaacaccgtc gccgacgcgt
    11881 acgccgagct cgtcgccgag ggctggctca cctcccgcca gggctccggc acccgcgtcg
    11941 ccgaccgggt cgccgccccc gccccccggc ccgtgcggac gaccgcgccg cccccgctcc
    12001 cgtacgacct cgtccagggc aagcccgacc ccggcgcctt cccccgcgcc gcgtggctgg
    12061 cggcggcccg gcgggccctg gcggccgcgc cgcacgaggc cttcgggatc ggcgacccgc
    12121 acggccggat cgaactgcgc ctggcgctcg ccgagtacct cgcgcgcgtg cggggcgtcc
    12181 gcaccgcccc ggaccggatc gtggtctgct ccggcgccgc gcacgcgctc cggctcctcg
    12241 cgcgcgtgct gggtccggcg gccccctggg cggtcgaggc gtacgggctg cccttccacc
    12301 gcgggctcct cgccgaggcc ggggtcacca ccctgccggt ggccgtcgac ggggacgggg
    12361 cgcgggtcgc cgagatcccg gagcacgcgc gcgcggtgct gctgaccccc gcccaccagt
    12421 tccccaccgg cggcccgctg cacccggagc gccgggccgg ggtcctggag tgggcgcggg
    12481 cctccggcgg tctggtgatc gaggacgact acgacgggga gttccgctac gaccggcggc
    12541 cggtgggcgc cgtccagggc ctcgaccccg agcggaccgc cctcatcggc tccgtcagca
    12601 agagcctctc ccccgccctg cggatcggct ggatgtgcct gccgtccgca ctggtggaac
    12661 cggtggtcgc ggcgaagggc cggcgcgagc cgtacgcctc cgcgacggac cagctcaccc
    12721 tcgcggacct cctcgccacg ggcgcgtacg accggcacgt ccggcggatg cgccagcggc
    12781 accgcgcgcg gcgcgaccgg ctgacggcgg tgctcgccga acgggtcccg gcggtacggg
    12841 tgacgggcat cgccgccggg ctgcacgccg tcctggaact gcccgcgggc acggagcggg
    12901 cggccctcgc cgcgacggcc cggcgcgggg tcgcggtgga ggggcttgcc ccctaccgcc
    12961 atcccggcgc ccgggacgcc accggccccg acgacccgga cggcccggac gacccggctg
    13021 ctcgggacgg ttcggctcct cgggacggtt cggctcctcg ggacggttcg gctcctccgg
    13081 tcgccccggg ccctccggac ggtctggtcg tcggctacgc gacgccgccg gagcggttgt
    13141 acccgggtgc gctggacgcc ctgtgcgcgg ggttgtccga ggcgctcggg tcctgacggg
    13201 cccccctcgc cgccttcgcc gcctaagccg cctaagccgc cgccaccttc gactccgccg
    13261 tcgtcaggat ctccgtgagg cggaggccga agcggacgtc gcaggcgtcg ggttcacccg
    13321 tgcgggccgc gtcgaccagg gcgtccaccg ccgcgccgaa cgcccccacc gccccgtccc
    13381 agcggggcat ttcggcccgg cccccggtgc cgtacagggt gagggccgcc tccgccgcct
    13441 ccggcggggc gctgagggtg agggtggcgg tgctgctcgc tcccgaggtg tggcggagca
    13501 ccaggtggtg ggtgtccgcc gggccgcgcg ccgcggtgat ctccgtgacg tcgccgagga
    13561 ccgggaggaa gaccgacagg acgtgcgggc ccacgtccca caggccgccc ttctcgcggc
    13621 gccagggcga ggccgcgtac gcgctggccg acccggtccc gtagagcgag cccagccagt
    13681 gcgcctcgcc caggaaccag ccgccggccg ccgtctgttc gtcgatccag cgggccgtcg
    13741 gctccgcgaa gcggagcgtg cagaagacca ccgaggccgc tcccgcggcc tccaccgcct
    13801 cggcgacctc ccgcgccccg gcgaccgtcg tcgccaccgg cttgtcgagg aggacgtggc
    13861 agccggcccg cgcggcccgg acggccagcg gggcctggac gtccggcggg agcgcgagcg
    13921 cgacggcgtc gctggcctcg aacagcgcgt ccacgtcgtc gtacgcgcgc gtgcccgagg
    13981 cggtggcgag cgcctcggcc gcctcggggc gtctgcccca caccccgctg aactcgactc
    14041 ccggatgccc cgccagcgcg ggtgcgtggg tgtgaccggc ccaggggccg gtgccgagga
    14101 gtccgatccg gggcttcgcg cccagctgct tcgccatgcc cccagtctgc cgggtgcctc
    14161 cgacagcggt ccccgcggac ccgcccgccc cgggccctcc gtgtaatccg ctcgcgtccc
    14221 tccccccgcc cggtgttagc gtttccgggc cggccgcggg actccggtgc gacttccgga
    14281 acctgcctct gaagcaacgt cttcgctgtt cgtgccctca ttccccggcc ggcatccacg
    14341 cggtacgaga aggagagagc cgtgtccgaa ctcgtcgccg ccgaggacgt gctcctcttc
    14401 gtcaacgccg ccgtgaccgc caccggccag cgcgagttcc gttcctccgc cgccgagcag
    14461 cggttctcgc tccgcttcgt ccacgagtac gtacgggtca actaccggcc cgtgtacgcc
    14521 gcggccctcg cgctcgacat caaccaccac aacgccgccc tcgtcgtcga gcagttgctg
    14581 cgcaccgccg acgaggcggg cgggcccgag gagaagcggg ccgaggggcg gctgatcgcg
    14641 gcccggctcg cgctgctgcc gccgcagcgg gtctaccggc tgttccgtac gctgcgcgcc
    14701 gccggggtca acaaccggcg tacgcgcgcg atcgtgcgcg ggtggctcgc ggcccgcccc
    14761 gatcccgccc tggacgcggt gaagtaccgg agcggcctga aggcctccct ccggcacgtc
    14821 cacgggcggc tgcccgaccc cgagaccggg gacttcctct tcgcgccggg gcgccgggtg
    14881 cggtacgaga acccgacgct cgacgcgttc cgccgcgccc ggtacgagca gggcgcgctg
    14941 tacgagctgc cgttcaccgt cgccgagggc ttcgccgcgg cgcacggcgt gccgcgcgcg
    15001 gtgttcctgg agcggatcgc gccccggatg acccggctcg aacagctgag gaccgagcgg
    15061 gccgccgatc tgtcggtgat gccgctgacc cggctcgccc tgtacgtcct ctcgctgccg
    15121 ttcggcgagc gcgtggaacg gcgggccgag ctgacggggg cgctgcgggc cgcgggccgg
    15181 cgggcggcgg ggccgtacgc cgggagctgg gggcgggtca cggcggtcct cgacgacagc
    15241 ttctcctcct cgggttcggc ggtgaagcgg cggcgcccgc tggccgtcgc gctcggctcc
    15301 caccatctgc tggaggccct cgcggcgccg ggggcgtaca ccccgctctg gacctcgggc
    15361 ggcgacgacc cgctgctcgt ccggccgtac ggggcgaccc cgctgggcct gcgggtcctg
    15421 gacggtctgg agaccggccc ggaccggctc gtgatcgtca cggacggctg ggacaacgcc
    15481 ccgccggggc tcgcgggcga ggtgctgcgg gtgtggcggt cgcggctgga tccggagcgc
    15541 cggacgagcg tggtgcacct gaaccccgta tacgacgcgc agggcttcga cgtgcgccgg
    15601 ctcgccccga gcgtgccggc ggccggcatc cgggacgcgg aggacctggc ggcgctggtg
    15661 gagatcgctc agttcgccga ggggcggacc gggttcgcgg agctgcgggc ctatctggac
    15721 cggcgggtgg agctgttcct gaaggcggcg gaggaagggg gccggggatg acccggatcg
    15781 atctgaccgg gctcgacgtc cggcccgcgc aggtgtgggg cggggtacgg ctggtgccgc
    15841 tggtgcggtc cgagccggtt cccgggctgc ggctgcaccg ggaggtgtac ggcgggttcg
    15901 gctcggtgga cctgccggac cgctccacgt acacctcgta cgtcccgcac gcgttcgtcg
    15961 ccgactggac gggggcgggg tccggggcgg gcccggcgcc gggggcgggc cgggagtccg
    16021 ccgcgtacgg gacccgactc ggcggcggag gcggcgggcg cggcgacggc ggccgtgacg
    16081 aggcgccgga ctgcgtgccc gtgcgccgtg tccaccggat ggcgaagcgg cggcgcgaca
    16141 aggacgggcg cgccgaggcg cggctgcgct tcctgccgct gcacctcgcg ctggaggggt
    16201 atctcgcgct gcacttcggc ggtccttcga tcgtctggga cgagtggtcc cgcgaggcgc
    16261 tccggcacgg cctctcgccg cgtgccgagg cggcgtacgc ggggtgggcc gtgcccgggc
    16321 tgacggaggc gctgcggatc ttcgaggtcc acccgggcca gtgcgggctg ctgctgtacg
    16381 tggccgactc gctggccgcg gccttcgtgg tgccgcaccc ggacgactac cggctgctgc
    16441 acccgacgct cgtggaggac ctgtacgggg agctggtcca ccagtacgcg tactacggcg
    16501 gtccggtgcc cgagttcacg gcgcggatcc gggacggggc cggcggcatc cgtaccctcg
    16561 cggacctgcg ggcggcggcc cggcagcagg agcgggcctg ggccgcggcg cacgacgggc
    16621 tcatggcgcg ggacctgctg gagaccccgt acgccttcga gcgggtccac cgggccgggc
    16681 cgttcgacct gcaccggttc ctgccgccgt tcgagcgggg cgggcgggag cagcacatcg
    16741 gggagctgat caccgaccgc aaggggcgca cggcctatct gaagacgttc cggctctcgg
    16801 agaaccagat ccgcaagggg tacctgctgc ggcggctcgc cgactgcgac tggcacctgg
    16861 ggcgggcggc ggaggccctg gggacctcgt acacggaggt cgtacggcgt gtccggagcg
    16921 cgggtctcgg tgccttcctg gacgagcact ccgtcgcacg gcggctccgc gaagatcggg
    16981 aaagctgagt aacacggggt tcacattcgg gcaaccgacg ggaaatcccg tgttgcgaag
    17041 ctgcgaccgg acacagacac cgcagcttct cgcagcgccg cgagccgccg cgacacccgc
    17101 agcagcacgc ccaaaggatg aggccagtga ccttcaaggc tgagtacatc tggatcgacg
    17161 gcaccgagcc gaccgcgaag ctccgctcga agacgaagat catcgtgggc gggggtgcca
    17221 cgctcggcga gctgccgatc tggggcttcg acggttccag cacgaaccag gccaagggcc
    17281 acgcctcgga ccgcgtcctc aagccggtct tcgtctgccc ggacccgatc cgcggcggcg
    17341 acgacgtcct ggtgctgtgc gaggtcctca acacggacat gacgccgcac gagtccaaca
    17401 cgcgtgccgc gctggccgag gtcgccgcga agtacgccgc tcaggagccg atcttcggca
    17461 tcgagcagga gtacacgttc ttcgagggca cccgcccgct cggcttcccg gtcaacggct
    17521 tcccggccgc ccagggcggc tactactgcg gtgtcggcgc ggacgagatc ttcggccgcc
    17581 cgatcgtcga ggcgcacctg gagaactgcc tcaaggccgg tctcggcatc tccggcatca
    17641 acgccgaggt catgccgggc cagtgggagt tccaggtcgg cccgctggcg ccgctggagg
    17701 tctcggacca gctgtggatc gcccgctggc tgctgtaccg cacggccgag gacttcaacg
    17761 tctccgcgac gctggacccg aagccggtga agggcgactg gaacggcgcg ggcgcgcaca
    17821 ccaacttctc gacgaaggcg atgcgcgagg gctacgacgc gatcatcacc gccgccgagt
    17881 ccctcggtga gggctcgaag ccgctggacc acgtcaagaa ctacggcgcc ggcatcgacg
    17941 accgcctgac gggcctgcac gagaccgccc cgtgggacca gtactcctac ggcgtctcgg
    18001 accgcggcgc ctcggtccgc atcccgtggc aggtcgagca ggaccagaag ggctacatcg
    18061 aggaccgtcg cccgaacgcg aacgtggacc cgtacacggt gacccgcctc ctggtggaca
    18121 cctgctgcga ggcgctcgcg aaggccggcc aggtctgatc cgcacccctg tacgccgaga
    18181 ggcgcccacc cttt
//
