LOCUS       NZ_LIVV01000003        10948 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02009 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIVV01000003
VERSION     NZ_LIVV01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02009
  ORGANISM  Streptomyces sp. CB02009
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 809130)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809130)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905825.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,214
            CDSs (total)                      :: 8,139
            Genes (coding)                    :: 7,984
            CDSs (with protein)               :: 7,984
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 51 of 155
            Pseudo Genes (incomplete)         :: 117 of 155
            Pseudo Genes (internal stop)      :: 15 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:06:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 554272
            Orig. end                         :: 565220
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVV01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10948
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10948
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10948
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5948](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5948
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            603..992
                     /locus_tag="AMK27_RS15190"
                     /old_locus_tag="AMK27_14960"
     CDS             603..992
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037145.1"
                     /locus_tag="AMK27_RS15190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14960"
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="WP_073906853.1"
                     /transl_table=11
                     /translation="MFHTGLRSGTAVQAAEEAGPDAELYFQRCRWCHTTTFQRLLCPTC
                     GSTDLNPELSEGEGVISVRRGVAAADADLWPVYMTEGFTVRCRVDGPLHAVRPGVRVKV
                     ASGVGVTGRRPVVRLCEEVPLDGWI"
     gene            complement(1086..2042)
                     /locus_tag="AMK27_RS15195"
                     /old_locus_tag="AMK27_14965"
     CDS             complement(1086..2042)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238294.1"
                     /locus_tag="AMK27_RS15195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14965"
                     /product="electron transfer flavoprotein subunit alpha/FixB
                     family protein"
                     /protein_id="WP_073906855.1"
                     /transl_table=11
                     /translation="MAEILVLVDHADGAVRKPALELLTLARRIGEPSAVVLGAGEAAAS
                     IAATAGEYGAATVYVADGAEFTEQLVVPKVDALTQLAKEKGAAAVLVTSSGEGKEIAAR
                     VALRLGSGLITDAVELEAGETGPVATQSVFAASYQVRSTVTHGVPVITVKPNSAAPEAS
                     SAAGTVENVTVSFTGNAARVVARTPRVSTGRPELTESAIVVSGGRGVGAAEGFAVVEKL
                     ADSLGAAVGASRAAVDAGWYPHSNQVGQTGKQVSPQLYVAAGISGAIQHRAGMQTSKTI
                     VAVNKDPEAPIFDLVDYGVVGDLFEVLPQLTDEIGAR"
     gene            complement(2081..2866)
                     /locus_tag="AMK27_RS15200"
                     /old_locus_tag="AMK27_14970"
     CDS             complement(2081..2866)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262570.1"
                     /locus_tag="AMK27_RS15200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14970"
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="WP_073906858.1"
                     /transl_table=11
                     /translation="MTLRIAVCVKYVPDATGDRGFADDLTVDRDEVDGLLSELDEYAVE
                     QALRISEESDDAEVTVVTVGPDDAKDALRKALSMGADKAVHVNDEDIHGSDVIATSAIL
                     AKALEKTGFDLVVCGMASTDGTMGVLPALLAERLSLPQVTLLSELSVEDGLVKGRRDGD
                     AATELLEAPLPAVVSVTDQSGEARYPSFKGIMAAKKKPVQELDLDDLDIDADEVGLAGA
                     WTLVDAVAARPARTKGTIVTDEGDGGKQLAAFLATQKFI"
     misc_feature    complement(2882..2896)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.3"
                     /tool="antismash"
     gene            complement(2895..4061)
                     /locus_tag="AMK27_RS15205"
                     /old_locus_tag="AMK27_14975"
     CDS             complement(2895..4061)
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037148.1"
                     /locus_tag="AMK27_RS15205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14975"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_107425576.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1e-30, bitscore:
                     102.0, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 6.2e-29, bitscore:
                     95.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.7e-50, bitscore:
                     166.4, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKDFDLYRPSEEHDMLRESVRALAEAKIVPFAAAVDEEGRFPQE
                     ALDALVANDLHAVHVPEAYGGAGADALATVIVIEEVARACGSSSLIPAVNKLGSLPVIL
                     SGSEELKHKYLGPLAKGDAMFSYALSEPDAGSDAAGMKTRAVRDGDFWVLNGVKRWITN
                     AGVSEYYTVMAVTDPDKRSKGISAFVVEKSDEGVSFGAPEKKLGIKGSPTREVYLDNVR
                     IPASRMIGAEGTGFATAMKTLDHTRITIAAQAIGIAQGALDYAKGYVTERKQFGKPIAD
                     FQGVQFMLADMAMKLEAARQLTYAAAAKSERVSAGGDKEDLTFFGAAAKCFASDVAMEV
                     TTDAVQLLGGYGYTRDYPVERMMRDAKITQIYEGTNQVQRIVMARNLP"
     gene            complement(4190..4870)
                     /locus_tag="AMK27_RS15210"
                     /old_locus_tag="AMK27_14980"
     CDS             complement(4190..4870)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238444.1"
                     /locus_tag="AMK27_RS15210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14980"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073906861.1"
                     /transl_table=11
                     /translation="MAQQARAIRTRRAILESAAAVFAERGYERTTIGEILVRAGVTKGA
                     LYFHFASKEDLALGVLDAQMLDEPLTPQPVKLQELVDQGFLLAHRLQRDALVRASVALA
                     LDSGATGVDRAAPFKAWIDQVSEVLTVAKARGELLVHVDVTDTAELFSGAFAGIQTMSQ
                     ILCDRKDLTHRVLVLLRHVLPTICTPALLTTLEMTEERARRLAAEYDLQRLKRGEGNLA
                     DTSG"
     misc_feature    4918..4931
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            5001..5948
                     /locus_tag="AMK27_RS15215"
                     /old_locus_tag="AMK27_14985"
     CDS             5001..5948
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037150.1"
                     /locus_tag="AMK27_RS15215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14985"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_073906864.1"
                     /sec_met_domain="AfsA (E-value: 5.1e-28, bitscore: 92.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQNRRGATTHVPGEFVHRADPADIFPTGWTQRGENRFSVSAHWP
                     ASHPFFSPVAGDRHDPVLVAETIRQATMLVAHAELGVPVDDQFVMWDLHFAADPAALAV
                     NGLPSNVTVDIHFPELGTRRGRARNLPTLVRLTRGGRPLATGGGLAGYTSAAAYRRLRG
                     DRMAALGRPVPLVQGVAPHLVGRRRPQDVVLGPGTRAGQWRLRVDTDHSTLFRRQNDHA
                     PGMMLLEAARQAATMTTGGVFLPTAVNVSFERYAELDSPCWIEAEVVPTPDRSTTAVRV
                     TGRQEGAPVFVCTLTSPSPDLAVAGAGLDGRLAG"
     gene            5963..6913
                     /locus_tag="AMK27_RS15220"
                     /old_locus_tag="AMK27_14990"
     CDS             5963..6913
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037151.1"
                     /locus_tag="AMK27_RS15220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14990"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_073906866.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.2e-11, bitscore:
                     37.2, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGPRILITGATGFIGARVASAAATAEEPAHVRLLARTPAAVPVEF
                     SGPGRETVPGDLRDPVSLRRACAGTDVLIHCASAIGGDAATARAVNDEGTRALVDAAAA
                     AGVTRIVSLSTASVHGRGPFRAAAPGTLPSAPVSATSRTRAAGDRHVMDAGGTVLRPHL
                     VYGSGDRQVIPGLVRLLGALPGPLDGGTSLHSLIDVDSLAGALLGAALSPATEPGAYYV
                     QHPDPVPVGELLAAGEELLARRTGTVRGAGVDVAAARALFAAVPFAQHHLDMLATDHWF
                     ADDRPWRELGCDPGPGFTEGFARHLPWYRGLLDHS"
     gene            6955..7719
                     /locus_tag="AMK27_RS15225"
                     /old_locus_tag="AMK27_14995"
     CDS             6955..7719
                     /NRPS_PKS="Domain: PKS_KR (7-186). E-value: 2.1e-09. Score:
                     29.5. Matches aSDomain:
                     nrpspksdomains_AMK27_RS15225_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037152.1"
                     /locus_tag="AMK27_RS15225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14995"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_073906868.1"
                     /sec_met_domain="adh_short (E-value: 3.4e-30, bitscore:
                     100.7, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSALAGRTAIVTGASRGIGRGIAQRLARDGALVAVHYGSNEEAAR
                     ETVGLIEERGGRAFAFRADLEAPDAVDTFYAGLDAGLAERGEDPGFDILVNNAAASGSG
                     RIQQLTPEVFDRLFTINVKAPLFLVQRGLDRLRDGGRIVNISSAVTKHAFPDSVTYAMT
                     KGAVDTMTLALAKQLGPRGITVNAVAPGYIATDMNARRRATPEASAALAALSVFNRLGA
                     PDDIADVVGFLVSDDARWITGQYVDATGGTAL"
     aSDomain        6976..7512
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK27_RS15225_PKS_KR.1"
                     /evalue="2.10E-09"
                     /label="AMK27_RS15225_PKS_KR.1"
                     /locus_tag="AMK27_RS15225"
                     /protein_end="186"
                     /protein_start="7"
                     /score="29.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TAIVTGASRGIGRGIAQRLARDGALVAVHYGSNEEAARETVGLIE
                     ERGGRAFAFRADLEAPDAVDTFYAGLDAGLAERGEDPGFDILVNNAAASGSGRIQQLTP
                     EVFDRLFTINVKAPLFLVQRGLDRLRDGGRIVNISSAVTKHAFPDSVTYAMTKGAVDTM
                     TLALAKQLGPRGITVN"
     CDS_motif       6982..7047
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AMK27_RS15225_0001"
                     /evalue="2.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="AMK27_RS15225"
                     /protein_end="31"
                     /protein_start="9"
                     /score="13.7"
                     /tool="antismash"
                     /translation="IVTGASRGIGRGIAQRLARDGA"
     gene            complement(7721..8368)
                     /locus_tag="AMK27_RS15230"
                     /old_locus_tag="AMK27_15000"
     CDS             complement(7721..8368)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985133.1"
                     /locus_tag="AMK27_RS15230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_15000"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073906870.1"
                     /transl_table=11
                     /translation="MTKQERAARTREALITSAAREFDQHGYTLAKLSAISTGAGVSPGA
                     LHFHFENKPAVAAAVEHEASTALRRTARTVYRQESDALQNLVDTTHSLARLLRDDVVVR
                     AGYRLSCSRGCRTDLNLRQEWQSCVQQRLAEAADEGLLATGHGGQQDLVRTVVAATVGL
                     EALCRENGEWLSPGTVTGLWRTLLPVLAAPGALAGLEPAGSRAVPAARAPVG"
     gene            complement(8758..10266)
                     /locus_tag="AMK27_RS15235"
                     /old_locus_tag="AMK27_15005"
     CDS             complement(8758..10266)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037198.1"
                     /locus_tag="AMK27_RS15235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_15005"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_073906873.1"
                     /transl_table=11
                     /translation="MTMEAFPGRRATVGGLIAVALALAGSVVLPTAPASAAGSTTYVDC
                     SRPAAGDGSQASPLNSIAQANGRTYAAGDTLAFASGTLCTGSLAPRGSGTATAPITLTR
                     YGTGALPVIDGGGAPDAVSLTDQDHWRISELKLTNPAASLARRTGLRISATDGKAHRGF
                     DIGNLVVDRVAGQTSKNSPTTEDFVQSAGIVTGASGTGSTLHDVRVHHNQISNTGGGGL
                     KIRVGALAVKGSGVLIEHNVVKDVGGDGIIASYADAPLIQYNNASGVGNGVYPFSGGNF
                     AGVWVLGDHNPTIQYNAVYGITRMSVADSQAFDCDWGNTGTCLIQYNFSRDNIGGFFLD
                     CDGCGTIGGAQQVIRYNVSENDCRMSSVSAGRSALRMYNNVLYCTDKKFSITLPDESVV
                     ENNIWVGTTDSRLPTAAGISWLWNVFQGVPRPTANGIVGDPHFVNPGTGGDSLASADGY
                     RLRSTSPALNNGSVISGSGGRDYWGNPVSATAKPHRGAYNGPGL"
ORIGIN
        1 ggcgagcgcc gactccgacg accccatggt ccgggtctgc ggcgccgccc ggctcgtcat
       61 gcggatgtac gccgagaacc cgaccttctc cgtgcatcgc taccggctca cccgcgaggt
      121 gaccgggctg cgcacgtacg agctgtcggt ggtctggcgg tacgagaaga ccctgggcga
      181 ctacctgcgc acccgctggg ccgaccgcag ggacggcacc ctgcgggcca acgtggccgc
      241 ggcggccgtg gtcgccgccc acaaccacgc cctgcggcac tggctccgct ccggaggcga
      301 gggcgacccg ctggcggagg tggacagcgc cctggagttc gtccgcggca cctggagccc
      361 cgacgcggtc gcggccgcgc cggccgagga ggccgaggac gtggtcgtgg tgatcacgaa
      421 gcggtccacc cccatgtggc gcgtggtgcg cgaggtggag tcgagcctcg gcgagagctg
      481 agagccgccc cagggatcgc ggtatctcta attcagggaa ctcaggtctt tatttgatgg
      541 cactcagtgc catatctttg ctccatgcac ggtcgagccg ccgagtgcga ggagaaggca
      601 tcgtgttcca cacgggactg aggagcggga ccgccgtcca ggccgccgag gaggcgggcc
      661 ccgacgccga gctctacttc cagcgctgcc gctggtgcca caccacgacg ttccagcgac
      721 tgctctgtcc gacctgcggg tcgaccgatc tcaaccccga actcagcgag ggcgagggcg
      781 tgatctccgt acggcgcggg gtcgccgccg ccgatgccga cctctggccg gtgtacatga
      841 cggagggctt caccgtccgc tgccgggtcg acgggccgct gcacgcggtg cgccccggcg
      901 tccgggtcaa ggtcgcctcc ggggtcggcg tgaccggtcg caggccggtc gtccggctct
      961 gcgaggaggt ccccctcgac gggtggatct gacccgccgg accgtcgcgc gggacagacg
     1021 gaaggggcgg gtggtggcac agagtgccac cacccgcccc ttccgcgtca tccggtgcgg
     1081 acgggtcagc gggcgccgat ctcgtccgtc agctgcggca gcacctcgaa gaggtcgccg
     1141 accacgccgt agtccaccag gtcgaagatc ggggcctcgg ggtccttgtt gaccgcgacg
     1201 atcgtcttcg acgtctgcat gccggcccgg tgctggatcg cgccggaaat gcccgccgcg
     1261 acgtacagct gcggcgagac ctgcttgccg gtctggccga cctggttgga gtgcgggtac
     1321 cagccggcgt ccaccgcggc gcgcgaggcg ccgacggccg caccgagcga gtcggccagc
     1381 ttctcgacga ccgcgaagcc ctcggccgcg ccgacacccc ggccacccga gaccacgatc
     1441 gcggactcgg tcagctccgg gcggccggtg gagacgcgcg gggtgcgcgc gacgaccctg
     1501 gcggcgttgc cggtgaagga gacggtgacg ttctcgaccg tgccggcggc cgaagaggcc
     1561 tccggagcgg cggagttcgg cttgaccgtg atgaccggca cgccgtgcgt gaccgtcgag
     1621 cgcacctggt acgaggcggc gaagacggac tgcgtggcga ccgggccggt ctcgccggcc
     1681 tccagctcca cggcgtcggt gatcagaccg gagccgaggc ggagcgcgac acgggccgcg
     1741 atctccttgc cctcgccgga ggaggtcacc aggacggcgg ccgccccctt ctccttcgcg
     1801 agctgggtga gcgcgtcgac cttcggcacg acgagctgct cggtgaactc ggcgccgtcc
     1861 gcgacgtaca cggtcgcggc gccgtactcg ccggcggtgg cggcgatcga ggccgcggcc
     1921 tcgccggcgc cgagcacgac cgccgagggc tcgccgatgc ggcgggccag ggtcagcagt
     1981 tcgagggccg gcttgcggac cgcaccgtcg gcgtggtcca cgagaaccag gatctcagcc
     2041 atgattcgtt gctcctgtgg ggtgtcaggc tgacggtcgg tcagatgaac ttctgggtgg
     2101 cgaggaaggc ggccagctgc ttgccgccgt cgccctcgtc cgtgacgatc gtgcccttgg
     2161 tgcgggccgg ccgcgcggcc accgcgtcga cgagggtcca cgccccggcg aggcccacct
     2221 cgtcggcgtc gatgtccagg tcgtccaggt ccagctcctg gaccggcttc ttcttggccg
     2281 ccatgatccc cttgaaggag gggtagcggg cctcgccgga ctggtcggtc accgagacga
     2341 ccgccggcag cggggcctcc aggagctcgg tggcggcgtc gccgtcgcga cggcccttca
     2401 cgagaccgtc ctcgacggag agctccgaca gcagggtgac ctgcggcagg ctcagccgct
     2461 cggcgagcag cgccggcagc acgcccatgg tgccgtcggt cgaggccatg ccgcagacga
     2521 ccaggtcgaa gccggtcttc tccagggcct tggcgaggat cgccgaggtg gcgatcacgt
     2581 cggagccgtg gatgtcctcg tcgttgacgt ggacggcctt gtcggcgccc atcgacagcg
     2641 ccttgcgcag cgcgtccttg gcgtcgtccg ggccgaccgt gacgacggtg acctcggcgt
     2701 cgtcggactc ctcggagatc cgcagggcct gctcgacggc gtactcgtcg agctccgaca
     2761 gcaggccgtc gacctcgtcg cggtcgaccg tcaggtcgtc ggcgaaaccg cggtcgccgg
     2821 tcgcgtcggg tacgtacttc acacagacag cgatcctcag ggtcacggcc tggctccttt
     2881 caagtgactg acgattaagg gaggttgcgg gccatgacga tgcgctggac ctggttggtg
     2941 ccctcgtaga tctgggtgat cttggcgtcg cgcatcatgc gctcgacggg gtagtcgcgg
     3001 gtgtagccgt agccgccgag gagctggacg gcgtcggtgg tgacctccat ggcgacgtcg
     3061 gaggcgaagc acttggcggc ggcgccgaag aaggtgaggt cctccttgtc gcctccggcg
     3121 gagacgcgct cggacttcgc ggcggcggcg taggtgagct ggcgggccgc ttcgagcttc
     3181 atggccatgt cggcgagcat gaactggacg ccctggaagt cggcgatggg cttgccgaac
     3241 tgcttgcgct cggtgacgta gcccttggcg tagtccaggg cgccctgggc gatgccgatg
     3301 gcctgggcgg cgatggtgat gcgggtgtgg tcgagggtct tcatcgcggt ggcgaagccg
     3361 gtgccctcgg cgccgatcat gcggctcgcg gggatgcgga cgttgtcgag gtagacctcg
     3421 cgggtcgggg agcccttgat gccgagcttc ttctccgggg cgccgaagga cacgccctcg
     3481 tcggacttct ccacgacgaa ggcggagatg cccttggagc gcttgtcggg gtcggtgacg
     3541 gccatcacgg tgtagtactc ggagacgccg gcgttggtga tccaccgctt gacgccgttg
     3601 aggacccaga agtcgccgtc gcgcacggcg cgggtcttca tcccggccgc gtccgagccc
     3661 gcgtcgggct ccgagagggc gtaggagaac atcgcgtcgc ccttggccag cgggccgagg
     3721 tacttgtgct tgagttcctc ggagccggac aggatcaccg gcagcgagcc cagcttgttg
     3781 accgccggga tcagcgagga cgaaccgcac gcacgggcga cctcctcgat cacgatcacc
     3841 gtggcgagcg cgtccgcgcc cgcgccgccg taggcctccg gcacgtgcac cgcgtgcagg
     3901 tcgttggcga ccagcgcgtc cagggcctcc tgcgggaacc ggccctcctc gtccaccgcc
     3961 gcggcgaacg gcacgatctt cgcctccgcg agcgcacgca ccgactcccg gagcatgtcg
     4021 tgctcctcgg agggccgata caggtcgaag tccttgccca tgatgtggag ctcccctaga
     4081 cgcttgtggc actgagtacc ctcagcaaaa cacactcagt gccatgacgc cagagcgccc
     4141 cgacgctgat ctgcgtcggg gcgctcaggg gaccggttgg tcggttttct taaccagacg
     4201 tgtccgcgag gtttccttcc cccctcttga gccgctgaag gtcgtactcg gcggccagcc
     4261 gtcgggcgcg ctcctcggtc atctcgagcg tggtcagcag ggccggggta cagatcgtcg
     4321 gcagcacgtg gcgaagcagc accagaacgc gatgggtaag gtctttgcga tcgcacagga
     4381 tctgggacat cgtctggatg cccgcgaagg cgcccgagaa cagctcggcc gtgtccgtca
     4441 cgtcgacatg gacgaggagc tcgccgcggg ccttcgccac cgtcaggacc tccgagacct
     4501 gatcgatcca ggccttgaac ggagccgccc ggtcgacccc ggtggcgccg ctgtcgaggg
     4561 ccagcgcgac actcgcccgc accagcgcgt cacgctgcag ccggtgggcg agcaggaagc
     4621 cctggtccac cagctcctgg agcttcacgg gctgcggcgt gaggggttcg tcgagcatct
     4681 gggcgtcgag cacgccgagg gccagatctt ccttggaagc aaagtggaaa tacagagccc
     4741 ccttggtcac cccggcgcgc accaagatct ccccgatggt cgtgcgctcg tagccacgct
     4801 ccgcgaagac ggccgcggcc gactccagga tcgcccgccg ggttcggatt gcacgcgctt
     4861 gctgtgccaa ggcgcctcct gctgtatcgc tgactgtgct cgacgtgtga cgtgaagcat
     4921 tgaaaagaaa accgcgctgt acgtattcta ttgagggcgg gtctgccgcc caggggtgcg
     4981 cggggacgcg agggggaacc atggatcaga accgccgagg agcgacgacg cacgtccccg
     5041 gggagttcgt ccaccgagcg gaccccgccg acatctttcc caccggctgg acgcagcggg
     5101 gagagaaccg attctccgtc tctgctcact ggcccgcctc acatcccttc ttctctccgg
     5161 tggccgggga ccggcacgac cccgttctgg tggccgagac gatacgccag gccaccatgc
     5221 tcgtcgctca tgcggaattg ggggtacccg tggacgacca gttcgtcatg tgggacctcc
     5281 atttcgccgc cgaccccgcg gccctcgccg tcaacggcct cccgtcgaac gtcaccgtgg
     5341 acatccactt ccccgaactg ggcacccgcc ggggccgtgc caggaacctg cccactctcg
     5401 tgcggctcac ccggggcggc cgcccgctcg ccaccggcgg cggcctcgcc gggtacacct
     5461 cggccgccgc ctaccgacgc ctccgcggcg accggatggc ggcgctcggc agacccgtcc
     5521 ccctggtcca gggggtcgct ccgcatctgg tgggccgccg ccgcccgcag gacgtcgtcc
     5581 tcggcccggg cacccgggcc ggccagtggc ggctgagggt cgacaccgac cactccacgc
     5641 tcttccggcg ccagaacgac cacgcgcccg ggatgatgct cctcgaagcc gcccggcagg
     5701 ccgcgacgat gacgaccggc ggtgtgttcc tccccaccgc cgtgaacgtc tccttcgagc
     5761 gctacgccga actcgacagc ccctgctgga tcgaggccga ggtcgtgccc acccccgacc
     5821 gctcgacgac cgcggtccgg gtcacgggcc ggcaggaggg cgcgccggtc ttcgtgtgta
     5881 cgctcacctc cccgtccccg gacctcgcgg tggccggagc cggcctcgac ggccggctgg
     5941 caggctgaac cggaacggaa cgatgggccc tcgcattctg atcaccggcg cgaccggatt
     6001 catcggcgcc cgggtggcgt ccgcggcggc gacggcggag gagcccgcgc atgtccgtct
     6061 gctcgcccgt acgccggcgg ccgtcccggt ggagttctcg ggaccgggga gggagaccgt
     6121 ccccggcgac ctcagggatc ccgtctcgct ccgtcgcgcc tgcgcgggga ccgacgtcct
     6181 gatccactgc gcctcggcga tcggcggcga cgcggcgacg gcccgcgcgg tcaacgacga
     6241 gggcacccgc gccctggtgg acgcggccgc ggcggccggg gtgacccgga tcgtctcgct
     6301 gagcaccgcg tccgtccacg gccgcggccc cttccgggcc gccgcccccg gcaccctgcc
     6361 gtcggcgccg gtctcggcga cgagccgcac ccgggcggcg ggcgaccggc acgtcatgga
     6421 cgccggcggg accgtcctgc ggccccatct cgtctacggg tcgggggacc ggcaggtgat
     6481 ccccggactg gtccggctcc tcggcgccct gcccggcccc ctcgacgggg gcacctcgct
     6541 gcactccctg atcgacgtcg acagcctggc gggcgccctg ctcggcgccg cgctctcgcc
     6601 ggcgaccgag ccgggcgcgt actacgtcca gcaccccgac ccggtcccgg tcggggaact
     6661 gctcgcggcc ggcgaggaac tgctcgcccg acggaccggg acggtccggg gcgcgggggt
     6721 ggacgtcgcc gccgcccgtg cgctgttcgc cgcggtcccc ttcgcgcagc accacctgga
     6781 catgctcgcc accgaccact ggttcgcgga cgaccgcccc tggcgggagc tcggctgcga
     6841 cccggggccg ggcttcaccg agggcttcgc gcgccacctc ccgtggtacc gcggcctcct
     6901 cgaccactcc tgaacagctc gaccactcct gaacagaagg aaggcgccac cgacatgtcc
     6961 gcgttggccg gcaggaccgc gatcgtcaca ggggcgagcc gaggcatcgg aagaggcatc
     7021 gcccagcggc tggcccggga cggagccctg gtggccgtcc actacggcag caacgaggag
     7081 gccgcccgcg agacggtggg gctcatcgag gaacggggcg gccgggcctt cgcgttccgc
     7141 gcggacctcg aggcacccga cgccgtcgac accttctacg ccggactgga cgcgggtctc
     7201 gcggagcgcg gcgaggaccc cgggttcgac atcctcgtga acaacgccgc cgccagcggt
     7261 tcgggccgca tccagcagct cacccccgag gtgttcgacc ggctgttcac catcaacgtg
     7321 aaggccccgc tcttcctcgt ccagcggggc ctcgaccggc tgcgggacgg cgggcggatc
     7381 gtcaacatct cctcggccgt gacgaagcac gccttccccg actcggtcac ctacgcgatg
     7441 accaagggtg ccgtcgacac catgaccctc gccctcgcga aacagctggg gccacggggg
     7501 atcacggtca acgccgtggc ccccggctac atcgcgacgg acatgaacgc gcggcgccgg
     7561 gcgaccccgg aggcgagcgc ggccctggcg gcgctctcgg tcttcaaccg cctcggggcg
     7621 ccggacgaca tcgcggacgt ggtcggcttc ctcgtctccg acgacgcgcg ctggatcacc
     7681 ggccagtacg tggacgccac gggagggacc gcgctctgaa tcagcccacc ggagcacgcg
     7741 cggcgggaac ggcccgactg cccgccggtt ccaggccggc gagtgctccg ggggcggcca
     7801 gcaccggcag gagcgtgcgc cacagtccgg tgacggtgcc gggggacagc cactcgccgt
     7861 tctcccggca cagtgcctcc agacccacgg tggcggccac caccgtgcgc accaggtcct
     7921 gctgaccgcc gtggccggtg gcgagcaggc cctcgtcggc cgcctccgcg agccgctgct
     7981 ggacgcagct ctgccattcc tggcgcagat tcagatccgt acggcagccc cgggagcagc
     8041 tgagccggta ccccgcgcgg acgacgacgt cgtcacggag cagccgggcc agggagtgcg
     8101 tggtgtcgac gaggttctgc agcgcgtcgg actcctgccg gtacacggtc cgggccgtcc
     8161 tgcgcagggc cgtggacgcc tcgtgctcga cggctgcggc caccgcgggc ttgttctcga
     8221 agtggaagtg gagcgcaccg gggctcaccc ccgcgcccgt gctgatcgcc gacagcttgg
     8281 ccagggtgta gccgtgctgg tcgaactccc tggccgcaga cgtgatcagc gcctcgcggg
     8341 tccgcgcggc ccgctcttgt ttggtcaccc gtggctcccg tagcaccgtg cgtcgcggtg
     8401 gcaccggcgt gcccgccccg tcgcgccgtt ccgttcgacg aatcaaaacc gactgcttgg
     8461 tattgtacgg gctttgcgta tgtctctgag cgcttcgacg cggctgggtg accgggaggc
     8521 ctcctggagg ggtacggaac gcttacgggt gaggcgagtt gaagggagac cgaaggcgcg
     8581 ggcgggtgcc ccctgttcca tggtcgatcc caacccgcaa accgggcaac aggtttgtcg
     8641 ccccctcaga agaggcctgg ccgctccccg gcgtacccct ctgccgtgct tcgccccatg
     8701 gccggcgacg acggacaccc ccggcaccgg cacgtgcgcg ccgggggtgc gggcgggtta
     8761 caacccgggg ccgttgtacg cgccgcggtg cggcttggcg gtcgcggaca ccgggttccc
     8821 ccagtagtcg cggcccccgc tgccgctgat cacggagccg ttgttcagcg ccggcgacgt
     8881 ggagcggagc cggtacccgt cggcggaggc cagcgagtcg ccgcccgtac cggggttcac
     8941 gaagtgcggg tcgcccacga tgccgttggc ggtcggcctc ggcacgccct ggaagacgtt
     9001 ccagagccag gagatccccg cggccgtcgg cagccgggag tcggtcgtgc ccacccagat
     9061 gttgttctcg acgacgctct cgtcgggcag ggtgatgctg aacttcttgt ccgtgcagta
     9121 gaggacgttg ttgtacatgc gcagcgcgga ccggcccgcg ctgacgctgc tcatccggca
     9181 gtcgttctcg gagacgttgt agcggatgac ctgctgggca ccgccgatgg tgccgcaccc
     9241 gtcgcagtcg aggaagaagc cgccgatgtt gtcgcggctg aagttgtact ggatcagaca
     9301 ggtgccggtg ttgccccagt cgcagtcgaa ggcctggctg tcggccacgg acatcctggt
     9361 gatcccgtac accgcgttgt actggatggt cgggttgtgg tcgccgagca cccagacgcc
     9421 ggcgaagttg ccgccggaga aggggtagac gccgttgccg acgccggagg cgttgttgta
     9481 ctggatcagg ggcgcgtcgg cgtagctcgc gatgatgccg tcgcccccga cgtccttgac
     9541 gacgttgtgc tcgatgagga cgccggagcc cttgaccgcc agggcgccga cgcggatctt
     9601 gaggccgccc ccgccggtgt tggagatctg gttgtggtgg acgcggacgt cgtggagggt
     9661 cgagccggtg ccgctcgccc ccgtgacgat cccggcggac tggacgaagt cctcggtggt
     9721 cgggctgttc ttgctcgtct ggcctgcgac acggtcgacg accaggttcc cgatgtcgaa
     9781 gccgcggtgc gccttcccgt cggtcgcgga gatccgcagt ccggtacggc gggcgaggga
     9841 ggcggcgggg ttcgtcagct tcagttcgct gatccgccag tggtcctgat cggtcaggga
     9901 gacggcgtcc ggtgcgccgc cgccgtcgat cacgggcagc gcgccggtgc cgtaacgcgt
     9961 cagtgtgatg ggggcggttg cggtgccgct gccccggggg gcgagggacc cggtgcagag
    10021 ggtgccggac gcgaaggcga gggtgtcccc ggccgcgtac gtccgcccgt tggcctgggc
    10081 gatgctgttc agcggcgagg cctggctgcc gtcgccggcg gccgggcgcg aacagtccac
    10141 gtaggtggtg ctcccggcgg ccgaggcggg cgcggtgggg aggacgacgc tgccggcgag
    10201 ggcgagcgcg acggcgatga gcccgccgac cgtcgcacgc cgccccggga atgcctccat
    10261 ggtcatgacg cgacaccttt ctcgaggtct ggtgccgcca gaagctaggt gcggccgatg
    10321 agcgagtcaa tgcagaagtg tcgaatgatc gaatatcgat caggatgcgg ttgattggtc
    10381 gacttcgggg ccggagcggt ccttccccgt cgggcggggg tgtgcgggga ggcggcctcc
    10441 agggggagct tcgtggaggc tccgggagcg ctccgggggg ggcggctcgg gacggctccg
    10501 ggggacggct cggggtggtg cccggtggct acagtggggg cacgatgaca cgtttccgcg
    10561 agagcgttcg gtccctgctg cgcgagcagg tgctcgacgc cgcccacggc ctcgtcgccg
    10621 ccgacggctg gagcgggctg cgcatgacct ccatcgcgcg cgccgccggt atcagccgcc
    10681 agaccctcta caacgagttc ggctcgaagg aggccatcgg caaggccctg gtccagcgcg
    10741 aactggaggg cttcctgctc ggcatccagg gtgaactcga cgcacatcgc ggcgagttgg
    10801 aggcggctgc cgccgccggg gtgggctaca cgctccagca ggccgtggat aatcccctcg
    10861 tcaagggcgt cctgctcgcg gaccgcggcg gcgaggacga cctcctcgcc tacctcacga
    10921 cccgcccgga accggtcttc gggacggc
//
