LOCUS       NZ_LIVV01000003        11413 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02009 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIVV01000003
VERSION     NZ_LIVV01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02009
  ORGANISM  Streptomyces sp. CB02009
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 809130)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809130)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905825.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,214
            CDSs (total)                      :: 8,139
            Genes (coding)                    :: 7,984
            CDSs (with protein)               :: 7,984
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 51 of 155
            Pseudo Genes (incomplete)         :: 117 of 155
            Pseudo Genes (internal stop)      :: 15 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:06:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 431535
            Orig. end                         :: 442948
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVV01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11413
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11413
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11413
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6413](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6413)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            751..2214
                     /gene="hemG"
                     /locus_tag="AMK27_RS14640"
                     /old_locus_tag="AMK27_14410"
     CDS             751..2214
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037013.1"
                     /locus_tag="AMK27_RS14640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14410"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_073906634.1"
                     /sec_met_domain="DAO (E-value: 1.3e-11, bitscore: 40.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNADTTGHHAGPHVVVAGGGIAGLAAAHRLAVAGHRVTLLEAGPR
                     LGGKLLAGEIAGAPVDLGAESLLARRPEAVALARAVGLGERLQAPGTTTASVWSRGELV
                     PMPKGHVMGVPGDARAVEGLLSAEGVRRIGLDAELPPTEIGDDIAIGAYVAARMGHEVV
                     DRLVEPLLGGVYAGDAYRISMKAAVPALFEAARTRPTLGDAVRAVQQAGAAVPADAAGA
                     ATGLGGSVFLGIDGGVGTLPGAVADAVRAAGGEILTEAPVTGLRRTGATGWQVVSGDRV
                     LTADAVVLATPAGPAAALLAPHAPTASAELDAIEYASMALVTLAFRRTDMPALAGSGFL
                     VPPVDGHTIKASTFSSQKWRWVTDRAPDLFVLRTSVGRHGEEEQVHREDADLVAASLKD
                     LAAATGLAARPVATAVTRWTGGLPQYPVGHLARVARVREAVAALPGLRVAGAAYDGVGI
                     PACIASAHRAADEIIATSKRADPGAGHSL"
     gene            2232..2945
                     /gene="hemQ"
                     /locus_tag="AMK27_RS14645"
                     /old_locus_tag="AMK27_14415"
     CDS             2232..2945
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="AMK27_RS14645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14415"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073906636.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRGGFADE
                     VQELFDQLAAKDITVRGTYDLSGLRADADVMIWWHAETSDELQEAYNLFRRTRLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADENPRDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWLLAFEADELHRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVAELVAGLA"
     gene            complement(3125..3868)
                     /locus_tag="AMK27_RS14650"
                     /old_locus_tag="AMK27_14420"
     CDS             complement(3125..3868)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432059.1"
                     /locus_tag="AMK27_RS14650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14420"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073907543.1"
                     /transl_table=11
                     /translation="MVWLPLLLVACVVAGVGCARLCRATLAAAAPAHGGVPAADAPELT
                     VGEAAYLAGGPLRVTDLTLVSMHRERRLLLARTGWATVVDADACAADPLERAVIGALGP
                     QGQAPVPAVRPLVAAAESVHLLAERLVARGLAVPAVGRREVAAGLRAVRAGLLLSLALG
                     TVSALLVPADERAPVLAGFALPLLASGLCLLIGRIEVYPHTRWASPAGQRLLASLSATD
                     PLTALATRGPAVLEPELRAALRGRG"
     gene            complement(3917..4999)
                     /locus_tag="AMK27_RS14655"
                     /old_locus_tag="AMK27_14425"
     CDS             complement(3917..4999)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037015.1"
                     /locus_tag="AMK27_RS14655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14425"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_079188437.1"
                     /transl_table=11
                     /translation="MLVNTLVTLYYLAGVTVIAVLVTRVVRTRGGPGGPVHDRYEAAFL
                     NGGPARVVDTALTALQADGRLAVGGPGIVAVVRPVAHDPVERAVFEAHAAAPHGALHHL
                     RMTVMRHPAVQETGDGLAVRGLVVDPATRRSTARWCGVAAVTTFVLFPVSIVLTVVGFA
                     TLDTDAGMPVPFIFKVLPILLATMVTALVCGSLTARQITPAGRRAVGAYGRAHAHLADA
                     GLLVALHGLRALPDPVLREQLAAAARIHPQPRRGRGGRRSAAQHRYDTSGADDDFAAAT
                     VWCAGTSACGGGGGGGGSSCSGGGSGSSCSSGACSGGGSSCGGGGSSCSSSSGSSCSSS
                     SGSSCSSSSSSGSSCSSSSS"
     gene            complement(5001..6413)
                     /locus_tag="AMK27_RS14660"
                     /old_locus_tag="AMK27_14430"
     CDS             complement(5001..6413)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037016.1"
                     /locus_tag="AMK27_RS14660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14430"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073906638.1"
                     /sec_met_domain="DUF692 (E-value: 3.8e-93, bitscore: 306.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTELGIGIGWRPEIADAVEGLSGIDWVEVVAENICADHLPDSLTR
                     LRERGVTVVPHGVSLGLGGADRPDPARLAALGEKAVALGAPLVTEHIAFVRAGGPLTAT
                     PQLEAGHLLPVPRTWDALDVLCENVRIAQDALPVPLALENIAALVSWPGEEMSEGRFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPARALSELPVEAIAYVHVAGGVERDGVWHDTH
                     AHPVPGAVLDVLADLASRVAPPGVLLERDDDFPPAEELADELRAIRRTVTAAGARSAAA
                     AGRGAAHQHGAPSSRTASRATLLIGAGARVSGGPMPGVAPEAPEAPEPETAPAPGARQR
                     VAVAQAALLSALVAGTPVPEGFDSRRLGVQSRALAAKRAGVVAKVAPELPEILGAGYRK
                     AFLAYARSRPMTGGYRRDALDFAEHLLVAGRPEDPAARRRLTEWWQDRSGSRPPGRATR
                     FVRAARHALVRK"
     gene            complement(6551..7258)
                     /locus_tag="AMK27_RS14665"
                     /old_locus_tag="AMK27_14435"
     CDS             complement(6551..7258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004950037.1"
                     /locus_tag="AMK27_RS14665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14435"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_107425569.1"
                     /transl_table=11
                     /translation="MSSNETDLRDEKPQFVLPLVVRIERDAPPSRTDALETAARAVLEI
                     LTDDRSLGEGEWAQAMTDWQDARIRKVVRRARGAEWRRAGALPGITVDGLESEVRVFPP
                     VPLDGWPKELAKLQVSGTDLDDPAAPGPVPEGAVVLWINPELEMSAGKTMAQAGHGAQL
                     LWWAMGDADRKAWHEAGFPLAVRTATAGEWPALTASGLPLVRDAGFTEIAPGPTVVAEG
                     SDRFAPGLRLPRP"
     misc_feature    7224..7238
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 15.7"
                     /tool="antismash"
     misc_feature    complement(7234..7249)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.56"
                     /tool="antismash"
     gene            7330..8004
                     /locus_tag="AMK27_RS14670"
                     /old_locus_tag="AMK27_14440"
     CDS             7330..8004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="AMK27_RS14670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14440"
                     /product="AIM24 family protein"
                     /protein_id="WP_073906642.1"
                     /transl_table=11
                     /translation="MKSDLFTGEHLAETADFPGMTLQNAKSVKYTVNGEMHARQGSMIA
                     FRGDLRFERKGQGIGGLLKRAVTGEGLALMAVRGQGEAWFAHEAQNCFIVEIEQGDAFS
                     VNGRNVLCFDATLHYEIKTVKGAGMTGGGLFNSVFSGYGKVALMCEGSPIVIPVTPQAP
                     VFVDTDAVVGWSEQLHTSLHRSQSVGSMIRGGSGEAVQLKLEGQGFVVVRPSELTPQKT
                     SN"
     gene            8001..9020
                     /locus_tag="AMK27_RS14675"
                     /old_locus_tag="AMK27_14445"
     CDS             8001..9020
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477442.1"
                     /locus_tag="AMK27_RS14675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14445"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_073906644.1"
                     /transl_table=11
                     /translation="MRLERVGRRHGLRGAWILREVDLDLPAGSLVRIVGANGTGKSTLL
                     RLVAGVDAPTTGRVTGRPPRTAYVPERFPAALPFTAADYLVHLGRIQGLGTAAARAGAG
                     EWLERFGAGEHARTPMAELSKGTSQKVAVAQAFLADPSLLVLDEAWTGLDTGARAELDA
                     VVRSRRAAGGTVVFVDHDPRRLEGEPSAVYEVREGRVGETGHPVAPAPGLTAAPPGLTS
                     GASGPVAPAPGPVAPAPGPTAAPPGLTSGASGSADPAPGPVRAGGGSGPRVRITVSGGP
                     HALPPPERLPGGPVVAPGPDGDGTLVLTVDADRSDALLSALLAASWHIHHLAPEGVRV"
     gene            9017..9667
                     /locus_tag="AMK27_RS14680"
                     /old_locus_tag="AMK27_14450"
     CDS             9017..9667
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238116.1"
                     /locus_tag="AMK27_RS14680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14450"
                     /product="ABC transporter"
                     /protein_id="WP_073906646.1"
                     /transl_table=11
                     /translation="MTALLRYQTGLLLRSQRWLAPLLLYAALLAVGVDAGEPVLGALGL
                     TAAGLLPVTAWSVRICVTQEPSAARNVVAAAAGRSRAHLAALVTGAGLPALVGAVAVLA
                     VTATGDRRGVTAPAAVTTGLLAVLACALTGAAVGALASRPFVRAPGWSVAALVLGSLLA
                     LVTTGSPAKHAMTALITGARTATADPPWLACGGAVLLAAACTALACRATARLE"
     gene            9682..9900
                     /locus_tag="AMK27_RS14685"
                     /old_locus_tag="AMK27_14455"
     CDS             9682..9900
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006345811.1"
                     /locus_tag="AMK27_RS14685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14455"
                     /product="hypothetical protein"
                     /protein_id="WP_073906648.1"
                     /transl_table=11
                     /translation="MNDTTESTASTSYTSYCAPRESGGPKPAPAAEGPFAACVLCQEAT
                     EYPETTKGITLCPVCEWREAERTACSG"
     gene            complement(9908..10774)
                     /locus_tag="AMK27_RS14690"
                     /old_locus_tag="AMK27_14460"
     CDS             complement(9908..10774)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890194.1"
                     /locus_tag="AMK27_RS14690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_14460"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_073907549.1"
                     /transl_table=11
                     /translation="MAALGLLGAVLVGCGREAVDTPPAPPAKEPAATAPSPRTSAPAAG
                     PKPARPPAAPPTMAPGPGGLTPVYTHRAKGTEKVVALTFDADMTADQGPRAAQGERFDN
                     PRLIETLRRLGVDATVFMTGRWAEEYPDQAKSIGRDPRFEIANHSYSHYAFASPCYGLP
                     TVSAPDMADDVQRAFDAFRDAGAVNVVPYFRFPGGCYDDASLRALAPAGVTAVQWDVVS
                     GDAFAQDADAVAEQVLDGVKPGSLVVMHCTRSAAPVTEQAIRRIVPELRARGYRFVKVS
                     ELMRGPL"
ORIGIN
        1 agggcccgca cccgcgccac gctcgcgcgc tgggtggcga tccggctctc cacgtccacc
       61 acctgctcgg tgacgtcctt ggcgtcggcc ttgcgggcca gcagcttccc cgtgccggcg
      121 agttcggtga ggacggagtc gtaccgctcc tgcggcaccc gcaggacgac gcgggaggtg
      181 acgtacccgt cgtcgagccg ctccgtcgac tcgttctcga cgcgtccgcc cgcgccggcc
      241 gtcacgtcgc gggccgtgac cagggccttg gtcacatcgc cgacctccac cgagagcgag
      301 gccgtgcgga tgacgtgctg ttgcgcggcg gccttcgacg gcgcggccga gggcgcggcc
      361 gaggcctgct cgccgcccga tcccgcgtag ccgtcggcgg ctccccgctc cgccgcgccc
      421 ttggcgtccg cgggcgcctc cgcgctcagg tcccgcttgg agtccccggc cgtgccgccg
      481 gctccgcagc cggtcagcac cagtgccgcg gtgagcaggg ccgcgcccgc caccgcccgt
      541 gttctcgccg tcgtacgtga gctccgcatc gaagtccccc caggggtggt aggtgtcgcc
      601 ggttcgacgt accggcccac cgatcgggtt gccctccggc ggtagcgatg cggtcacggt
      661 ccggcctcgg aacgggagcg tgccggaacg ggagcgggcc ggagggagcc tcggatccgg
      721 agccgatggg cgatttgaga gagtggtccc atgaacgcgg acaccacggg acaccacgcc
      781 gggcctcatg tcgtcgtcgc gggaggcggt atcgccggac tcgccgccgc ccaccggctg
      841 gccgtcgccg gccaccgggt gaccctgctc gaagcgggcc cgcggctcgg cggcaagctc
      901 ctcgccgggg agatcgcggg cgcccccgtg gacctcggcg ccgagtcgct cctcgcccgc
      961 cgtcccgagg cggtcgccct ggcccgggcc gtcggcctcg gggagcgcct ccaggccccc
     1021 gggacgacca ccgcgtccgt ctggtcccgg ggcgagctcg tccccatgcc caagggccac
     1081 gtcatgggcg tccccggcga cgcccgcgcc gtcgagggcc tgctctccgc cgagggcgtg
     1141 cgccggatcg gcctcgacgc cgaactgccc ccgaccgaga tcggcgacga catcgcgatc
     1201 ggcgcgtacg tcgccgcccg gatgggccac gaggtcgtcg accggctcgt ggaacccctg
     1261 ctcggcgggg tgtacgccgg tgacgcctac cgcatctcga tgaaggccgc cgtccccgcc
     1321 ctcttcgagg ccgcccgcac ccggcccacg ctcggcgacg ccgtccgcgc cgtccagcag
     1381 gccggggccg ccgtccccgc cgacgcggcg ggcgccgcca ccgggctcgg cggctccgtc
     1441 ttcctcggca tcgacggcgg cgtcggcacc ctgcccggtg ccgtcgccga cgcggtccgc
     1501 gccgccggcg gcgagatcct caccgaggcc cccgtgaccg ggctccggcg caccggggcc
     1561 accggctggc aggtcgtgag cggcgaccgg gtcctcaccg ccgacgccgt cgtcctcgcc
     1621 acccccgccg gaccggccgc cgcgctgctc gccccgcacg cgcccaccgc ctccgccgag
     1681 ctcgacgcga tcgagtacgc ctccatggcc ctggtcacgc tggccttccg ccgcaccgac
     1741 atgcccgccc tggcgggctc ggggttcctc gtgccgcccg tcgacggcca cacgatcaag
     1801 gcctccacct tctcctcgca gaagtggcgg tgggtcaccg accgggcccc ggacctgttc
     1861 gtgctgcgga cctccgtcgg ccgccacggc gaggaggagc aggtccaccg cgaggacgcc
     1921 gacctcgtcg ccgcctcgct caaggacctc gccgccgcga ccgggctcgc cgcccggccc
     1981 gtcgccaccg ccgtcacccg ctggacgggc ggcctgccgc agtacccggt cggccacctc
     2041 gcccgggtcg cccgggtccg cgaggccgtc gcggccctcc ccggactgcg tgtcgcgggc
     2101 gccgcgtacg acggtgtcgg catccccgcc tgcatcgcgt cggcccatcg cgcggccgac
     2161 gagatcatcg ccacgtcgaa aagggccgac cccggtgcgg ggcactccct gtgacgggcg
     2221 agaataggcg tatgagtgcg cccgaaaaga tcccgaacgc cggtaagaag gcgaaggacc
     2281 tcaacgaggt catccgctac accctgtggt ctgtcttcaa gctgcgcgac gttctccccg
     2341 aggaccgcgg cggcttcgcc gacgaggtcc aggagctgtt cgaccagctc gcggccaagg
     2401 acatcaccgt ccgcggcacg tacgacctct ccggtctgcg cgccgacgcc gacgtgatga
     2461 tctggtggca cgccgagacg agcgacgagc tccaggaggc gtacaacctc ttccgccgca
     2521 cccgcctggg ccgcgccctg gagccggtct ggtcgaacat ggcgctgcac cgccccgccg
     2581 agttcaacaa gtcgcacatc ccggccttcc tggccgacga gaacccgcgc gactacgtct
     2641 cggtctaccc cttcgtgcgc tcctacgact ggtacctgct gccggacgag gaccgccgcc
     2701 ggatgctcgc ggaccacggc aagatggccc gcggctaccc ggacgtccgt gccaacacgg
     2761 tcgcctcctt ctccctcggc gactacgagt ggctcctcgc cttcgaggcc gacgagctgc
     2821 accgcatcgt ggacctcatg cgtcacctgc gcgcctccga ggcgcggatg cacgtccgcg
     2881 aagaggtccc gttctacacc ggccgtcgca agagcgtcgc cgagctggtg gcgggcctgg
     2941 cctgacggcc gtacgaaaac ctggtggtgg cccggcttcc ggccgggcgt gccacccctc
     3001 gacccggaag atcagacggc aggcgtgacc atggcctgcc cgtccagcga caccgggtcc
     3061 ggctccgggt gcggcgcgca tgacgcgcgc cgcaccggcg tcatttccgg gacctttccg
     3121 gctctcagcc ccgcccgcgc agcgccgccc ggagctccgg ctccaggacc gcgggtccgc
     3181 gcgtcgcgag ggccgtcagg ggatcggtgg ccgagagcga ggcgagcagc cgctgcccgg
     3241 ccggcgaggc ccagcgggtg tgcgggtaca cctcgatccg cccgatcagc aggcagagcc
     3301 ccgacgcgag cagcggcagc gcgaaaccgg cgaggaccgg ggcccgctcg tcggccggca
     3361 cgagcagcgc ggacaccgtc ccgagggcca ggctgagcag gaggccggcc cgtaccgccc
     3421 gaagcccggc ggcgacctcc cgccggccca cggcgggcac ggcgagcccc cgggccacca
     3481 gccgctccgc gagcaggtgg acggactcgg cggcggcgac cagcggacgt acggcgggga
     3541 ccggcgcctg accctgaggg cccagggcgc cgatcacggc ccgctccagc gggtccgccg
     3601 cgcaggcgtc ggcgtccacg accgtcgccc agccggtgcg ggcgagcagc agccgccgct
     3661 cccggtgcat ggacacgagg gtgagatcgg tgacccgcag cggtccgccg gcgaggtagg
     3721 ccgcctcgcc gacggtgagc tcgggcgcgt ccgccgcggg cacgccgccg tgcgcgggcg
     3781 cggccgccgc gagcgtcgcc cggcacagcc gcgcgcagcc gacgccggcc accacgcacg
     3841 ccacgagcag caacggaagc cagaccatga ccgagttgta cgggaggcgg ggcgggcccg
     3901 ccacccgaac gggtgatcag ctgctggagc tggaacacga gctcccgctg gaactggagc
     3961 tggagcagga actcccactg gaactggagc aggaactccc actggaactg gagcacgagc
     4021 tgccgccccc gccgcagctg gagccgcccc cggaacaggc gccggaggag cacgacgacc
     4081 cggagccgcc gcccgagcag gagcttccgc ccccgccacc tccgccgccg cacgcgctgg
     4141 tccccgcaca ccacacggtg gccgcggcga agtcgtcgtc cgcgcccgag gtgtcgtagc
     4201 ggtgctgcgc cgcggaacgc cgtccgccgc gtccacgccg gggctgcgga tggatccgtg
     4261 cggcggccgc cagctgctcc cgcagcaccg gatcgggcag cgcacggagc ccgtgcaggg
     4321 ccaccagcag gccggcgtcc gcgagatggg cgtgggcccg cccgtacgcc cccaccgcgc
     4381 gccgcccggc cggggtgatc tgccgggccg tcagcgaccc gcagacgagg gcggtgacca
     4441 tggtggcgag caggatcggc agcaccttga agatgaacgg gaccggcatc cccgcgtcgg
     4501 tgtccagggt cgcgaagccc acgaccgtga ggacgatcga caccgggaag agcacgaagg
     4561 tcgtgacggc ggccacgccg caccaccggg ccgtggagcg ccgggtcgcc gggtcgacga
     4621 cgagcccgcg caccgcgagc ccgtcaccgg tctcctggac cgccgggtgc cgcatcacgg
     4681 tcatccgcag gtggtgcagg gccccgtgcg gggcggcggc gtgcgcctcg aacacggccc
     4741 gctccaccgg gtcgtgcgcg acggggcgga cgacggccac gatgccgggg ccgccgaccg
     4801 ccagcctgcc gtcggcctgg agggcagtca gggcggtgtc gacgaccctg gcgggcccgc
     4861 cgttgaggaa ggcggcctcg taccggtcgt ggaccggtcc gccgggtccc ccgcgggtcc
     4921 ggacgacccg ggtgacgagc accgcgatga cggtgacgcc ggcgaggtag tagagggtga
     4981 ccagggtgtt cacgagcacc tcacttccgt accagtgcgt ggcgggcggc ccgcacgaac
     5041 cgggtggcgc ggcccggcgg ccggctgccc gaacggtcct gccaccactc cgtgagccgc
     5101 cgccgcgccg ccggatcctc cggccgcccg gcgacgagga ggtgctcggc gaagtccagc
     5161 gcgtcacggc ggtagccgcc ggtcatgggc cgggacctgg cgtacgcgag gaacgccttc
     5221 cggtagcccg cgcccaggat ctcggggagc tccggcgcca ccttcgcgac gaccccggcc
     5281 cgcttggccg cgagcgcccg gctctgcacg ccgagccgcc ggctgtcgaa gccctcgggc
     5341 acgggcgtcc cggcgacgag cgcggacagc agagcggcct gggcgacggc gacgcgctgc
     5401 cgggcgccgg gcgcgggcgc ggtctccggt tcgggggcct cgggtgcctc cggggcgacc
     5461 ccgggcatcg gcccgccgga gaccctggcc ccggccccga tgagcagcgt cgcccgggac
     5521 gcggtcctcg acgacggggc gccgtgctgg tgggccgcgc cccgccccgc cgccgcggcg
     5581 gaacgcgcgc ccgcggcggt gaccgtccgg cggatcgccc gcagctcgtc ggccagctcc
     5641 tccgcgggcg ggaagtcgtc gtcccgctcc agcagcacgc ccggcggcgc gacccgcgag
     5701 gcgaggtccg ccaggacgtc gaggaccgcg ccgggcaccg gatgcgcgtg ggtgtcgtgc
     5761 cagaccccgt cccgctcgac accccccgcg acgtgcacgt acgcgatcgc ctccaccggc
     5821 agctccgaca gggcccgcgc ggggtcctcg ccccggttga cgtggttggt gtgcaggttg
     5881 gcgacgtcga tcaggagccg tacccccgta cgctccacca gctccgccag gaagcgtccc
     5941 tccgacatct cctcgccggg ccacgagacg agcgcggcga tgttctccag ggcgagcggc
     6001 acgggcagcg cgtcctgcgc gatccgcacg ttctcgcaga gcacgtcgag cgcgtcccag
     6061 gtcctgggta cgggaagcag gtgacccgct tcgagctgcg gggtcgcggt gagcggcccc
     6121 ccggcccgga cgaacgcgat gtgttccgtg accagcggcg cccccagcgc caccgccttc
     6181 tcgccgagcg cggcgagccg cgccgggtcg gggcggtccg cgcccccgag ccccagcgag
     6241 acgccgtgcg gcacgacggt gacgccgcgc tcgcgcagcc gcgtcaggga gtcgggcaga
     6301 tggtcggcgc agatgttctc cgcgacgacc tccacccagt cgattccgga cagcccctcc
     6361 accgcgtcag cgatctcggg acgccagccg atgccgatgc cgagttccgt catgtccccc
     6421 tccttcggtc ctcagtcacc cacgtgatgg ccccggcggg agcgttcgaa ccccgggagg
     6481 gtcacgttca gaggttgatt tgaggttccg ttcggtcacg gcggacggag ggggtgccgg
     6541 gcggtgacgg ctacggccgc ggcagccgca gccccggggc gaaccggtcg ctgccctcgg
     6601 ccaccacggt ggggccgggg gcgatctccg tgaagcccgc gtcccggacc agcgggagac
     6661 cgctcgccgt cagggcgggc cactccccgg ccgtcgcggt gcggaccgcc agcgggaagc
     6721 ccgcctcgtg ccaggccttc cggtcggcgt cccccatcgc ccaccacagc agctgcgccc
     6781 cgtgcccggc ctgggccatc gtcttgcccg ccgacatctc gagctccggg ttgatccaca
     6841 gcaccaccgc gccctcgggc acggggccgg gcgcggccgg gtcgtcgagg tcggtgcccg
     6901 agacctggag cttggccagt tccttgggcc agccgtcgag cgggaccgga gggaagaccc
     6961 gcacctccga ctccaggccg tcgaccgtga tccccgggag cgcgcccgcc cgccgccatt
     7021 ccgcgccgcg cgcccggcgc accaccttgc ggatcctggc gtcctgccag tcggtcatgg
     7081 cctgtgccca ctcgccctcg ccgagcgagc ggtcgtccgt gaggatctcc aggacggcgc
     7141 gcgcggcggt ctccagggcg tcggtacggg acggcggcgc gtcccgttcg atccgtacga
     7201 ccaacggcag gacgaactgg ggcttctcat cacgaagatc ggtctcattg ctgctcaccg
     7261 ggcaattctg ccactgccgc cacaagcgcc tcttggagga atgagcagtt ccgggccagg
     7321 atgcccctca tgaagagtga tcttttcacc ggcgaacacc tcgccgagac cgcagatttt
     7381 ccggggatga ccctccagaa cgccaaatcc gtcaaataca ccgtcaacgg ggagatgcac
     7441 gcccgccagg gctccatgat cgccttccgc ggcgatctcc ggttcgagcg caagggccag
     7501 ggcatcggcg gcctgctcaa gcgggccgtc accggcgagg gcctggccct gatggccgtg
     7561 cgcggccagg gcgaggcgtg gttcgcgcac gaggcgcaga actgcttcat cgtcgagatc
     7621 gagcagggcg acgcgttctc ggtcaacggc cgcaacgtgc tctgcttcga cgccaccctc
     7681 cactacgaga tcaagaccgt gaagggcgcc ggcatgaccg gcggcggcct cttcaactcc
     7741 gtcttctccg gctacggcaa ggtcgccctc atgtgcgagg gcagccccat cgtcatcccc
     7801 gtcacgccgc aggccccggt gttcgtcgac accgacgccg tggtcggctg gagcgagcag
     7861 ctgcacacct cgctgcaccg ctcgcagtcc gtcggctcga tgatccgcgg cggctcgggc
     7921 gaggcggtgc agctgaagct ggagggccag ggcttcgtgg tcgtccggcc cagcgagctg
     7981 accccgcaga agacctcgaa ttgaggctgg agcgggtcgg gcggcggcac ggcctccggg
     8041 gcgcgtggat cctccgcgag gtggacctcg acctgcccgc cgggagcctc gtccggatcg
     8101 tcggcgcgaa cggcaccggg aagtcgaccc tgctccggct cgtcgccggt gtcgacgcgc
     8161 ccaccacggg acgcgtcacg ggccgcccgc cccgcacggc gtacgtcccc gaacgcttcc
     8221 cggccgcgct gcccttcacg gccgcggact acctggtcca cctggggcgg atccaggggc
     8281 tcggcacggc ggccgcgcgg gccggggcgg gggagtggct ggagcgcttc ggggccggcg
     8341 agcacgcccg taccccgatg gcggagctct ccaagggcac cagccagaag gtcgccgtgg
     8401 cccaggcctt cctcgccgac ccgtcgctcc tggtcctcga cgaggcctgg accggcctcg
     8461 acacgggcgc gcgtgcggag ctggacgcgg tggtccgctc acgccgggcg gcgggcggaa
     8521 cggtcgtctt cgtcgaccac gacccgcgcc ggctggaggg ggagccgtcc gcggtgtacg
     8581 aggtgcggga gggacgggtc ggcgagaccg gacatcccgt cgccccggct ccgggcctga
     8641 cggctgcgcc cccgggcctg acctccgggg cctcgggtcc ggtcgccccg gctccgggtc
     8701 ccgtcgcccc ggctccgggc ccgacggctg cgcccccggg cctgacctcc ggggcctcgg
     8761 gctccgccga ccccgccccg ggtccggtcc gcgcgggtgg cgggtcgggg ccgcgggtgc
     8821 ggatcaccgt ctcgggcggt ccgcacgccc tcccgccccc ggaacgactg cccggcggcc
     8881 ccgtcgtcgc acccgggccc gacggcgacg gcaccctcgt cctcaccgtc gacgccgacc
     8941 ggtccgacgc cctgctgagc gcgctcctcg ccgcctcctg gcacatccac cacctcgccc
     9001 ccgaaggcgt acgcgtatga cggcgctcct gcgctaccag accggcctgc tgctccgctc
     9061 gcagcgctgg ctcgccccgc tcctcctgta cgcggccctc ctcgccgtcg gcgtcgacgc
     9121 gggcgagccc gtcctcggcg cgctcggact cacggccgcc gggctgctcc ccgtcaccgc
     9181 ctggtcggtc cggatctgcg tcacgcagga gccgtccgcc gcacggaacg tcgtcgcggc
     9241 cgcggccggg cggagccgcg cgcacctcgc cgcgctggtc accggggcgg gtctgcccgc
     9301 cctcgtcggg gcggtcgcgg tcctcgcggt gacggcgacc ggcgaccggc gcggtgtgac
     9361 cgcgcccgcc gccgtgacca cgggcctcct ggcggtgctg gcctgcgccc tgacgggcgc
     9421 cgccgtcggc gcgctggcgt cgcgcccctt cgtccgggcg cccggctggt ccgtcgcggc
     9481 cctcgtcctg ggctcgttgc tcgccctggt gaccaccggc tcaccggcga agcacgcgat
     9541 gacggccctg atcaccggcg cccgcacggc gaccgccgac ccgccgtggc tcgcctgcgg
     9601 gggcgcggtg ctcctcgcgg cggcgtgcac ggccctcgcg tgccgggcca cggcacgcct
     9661 ggagtgagta cgctcgaaga catgaacgac acgaccgagt ccactgcgtc cacgtcgtac
     9721 acgtcgtact gcgccccccg ggagtccggc ggtccgaagc ccgcaccggc cgccgagggc
     9781 cccttcgccg cctgtgtgct gtgccaggag gcgaccgagt acccggagac cacgaagggc
     9841 atcaccctgt gcccggtctg cgagtggcgc gaggcggagc ggacggcctg ctcgggctag
     9901 gcccgaccta gagggggccg cgcatcagtt ccgagacctt cacgaagcgg tagccgcggg
     9961 cgcggagttc cgggacgatg cgccggatcg cctgctccgt gaccggggcc gcgctgcgtg
    10021 tgcagtgcat gaccaccagg gagcccggct tcaccccgtc gagcacctgc tccgccaccg
    10081 cgtccgcgtc ctgcgcgaag gcgtcgccgc tgaccacgtc ccactggacc gccgtcaccc
    10141 cggccggggc gagcgcgcgg agggacgcgt cgtcgtagca gccgccgggg aagcggaagt
    10201 acgggacgac gttcaccgcc ccggcgtcgc ggaacgcgtc gaaggcccgc tggacgtcgt
    10261 ccgccatgtc gggagcggag acggtcggca ggccgtagca gggggacgcg aaggcgtagt
    10321 ggctgtagga gtggttggcg atctcgaagc gcgggtcgcg gccgatggac ttcgcctggt
    10381 ccgggtactc ctccgcccag cggcccgtca tgaacacggt cgcgtccacc ccgagccggc
    10441 gcagggtctc gatgagccgc ggattgtcga aacgctcgcc ctgcgcggcc cggggcccct
    10501 gatcggccgt catgtcggcg tcgaaggtga gcgcgacgac cttctccgtg cccttcgccc
    10561 ggtgcgtgta cacgggggtg aggccgccgg gccccggcgc catggtgggc ggcgcggcgg
    10621 gcgggcgcgc cggcttggga ccggccgccg gggcgctcgt ccggggcgac ggggcggtgg
    10681 cggccggttc cttcgccggc ggggccgggg gagtgtcgac cgcttcccgg ccgcagccga
    10741 cgaggacggc gcccagaaga ccgagcgccg ccaggtttcg tacagaagtg ttcacgaccg
    10801 gaagttaacc gatcaaagtg gctcaggcgt gcaacccggc gacggcagcg ccccgtgccg
    10861 ctcacgtggc cagcagccgc ctgcggctct cctccacgtc gaagtcaccc ggcgggtacg
    10921 ccgggtccag cgcctccagg tgttcgagca gcagcttgct gatcgcccag ttccgatacc
    10981 acttgcggtc cgccgggacc aggtaccagg gcgccgcctc cgtcgtgcag cgcttcaggg
    11041 cgaccgcgta cgcctgctgg tacgccggcc acaccgcgcg ctcctcgatg tcgcccacgt
    11101 tgaacttcca gtgcttctcc gggttgtcca ggcgctccag cagacgggcg cgctgttcct
    11161 cgtacgagag gtgcaggaag accttcacga ccgtcacgcc gtcgtcggcg agggtcttct
    11221 cgaaccggtt gatctggccg taacggcggt tcagctgcgt gggcgcgacc agctcccgca
    11281 cccgggcgat cagcacgtcc tcgtagtgcg agcggtcgaa gatgccgatc tcgcccggac
    11341 gggggagcgc cttcatgatc cgccacagga acgggtggct ctgctcctcc ggcgtcgggg
    11401 ccttgaaggc gcg
//
