LOCUS       NZ_LIVV01000002        10867 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02009 scaffold2, whole genome shotgun sequence.
ACCESSION   NZ_LIVV01000002
VERSION     NZ_LIVV01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02009
  ORGANISM  Streptomyces sp. CB02009
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1350328)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350328)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905825.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,214
            CDSs (total)                      :: 8,139
            Genes (coding)                    :: 7,984
            CDSs (with protein)               :: 7,984
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 51 of 155
            Pseudo Genes (incomplete)         :: 117 of 155
            Pseudo Genes (internal stop)      :: 15 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:06:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 249282
            Orig. end                         :: 260149
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVV01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5867](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5867
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(229..1065)
                     /gene="fdhD"
                     /locus_tag="AMK27_RS08550"
                     /old_locus_tag="AMK27_08380"
     CDS             complement(229..1065)
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="fdhD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037709.1"
                     /locus_tag="AMK27_RS08550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08380"
                     /product="formate dehydrogenase accessory sulfurtransferase
                     FdhD"
                     /protein_id="WP_073904425.1"
                     /transl_table=11
                     /translation="MGRVTVRRRVLRVREGDSSYRPDTLAAEEPMEIRVGGRPLTVTMR
                     TPGDDFDLAVGFLVSEGVVHSAEDVAGIRYCAGATADGGNTYNVVDVGLAPGVAAPDAS
                     LERNFYTTSSCGLCGKASLDAVRTTAAWSVAEDPLRIGTDVLAALPDRLRAAQRVFDTT
                     GGLHAAGLFTAEGELVCLREDVGRHNAVDKVVGHALREGLLPLRESVLMVSGRASFELV
                     QKAVMAGIPLLAAVSAPSSLAVDLAAESGLTLVGFLRGSSMNVYTGDERLGSVPVA"
     gene            1247..1429
                     /locus_tag="AMK27_RS08555"
                     /old_locus_tag="AMK27_08385"
     CDS             1247..1429
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMK27_RS08555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AMK27_08385"
                     /product="hypothetical protein"
                     /protein_id="WP_073904427.1"
                     /transl_table=11
                     /translation="MPATLSSGTRTLVSGQYAVVELRETDDAAAGELWPAEIDPTELAA
                     AERGYLLLEHLARRG"
     gene            complement(1437..3707)
                     /locus_tag="AMK27_RS08560"
                     /old_locus_tag="AMK27_08390"
     CDS             complement(1437..3707)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843023.1"
                     /locus_tag="AMK27_RS08560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08390"
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="WP_198955850.1"
                     /transl_table=11
                     /translation="MGPTPEGDPEFHPYHHPAAGWGAARSVTGFLVREGALVDGPRAIM
                     RMNHENTGFDCPGCAWPDDTKGLHLDICENGIKHVTWEMTRKRVGGEFFAAHSVSELAE
                     WSDYDLENEGRLTEPMVYDPESDHYVPITWKDAFEVVGRALRGLDNPHQASFYTSGRLG
                     NEATFLYQLMARELGTNNLPDCSNMCHEASGRALQASLGTGKGTVDLKDWETADALFIF
                     GVNAASNAPRMLTALAEAHRRGAQIVHVNPLVEAAATRTIVPHDFTDMALFKTTKTSTL
                     NLQPRIGGDMALIRGMAKAVLEQSATDPKALDQEFVDRHTSGFEEYRALCEATPWEEIE
                     RQSGLSRADILKAARVYGEADRSIVSWCLGLTQHEHGVDTVREIVNLLLLRGNLGREGA
                     GPSPVRGHSNVQGNRTCGIDHRPSEAFLDRLAEVCEIEPPREHGLDTVRTIEAMQRGEI
                     KVFVGMGGNFALAAPDTPLTYAALRTCDLTVQVSTKLNRSHLVHGKQALILPCLGRTEK
                     DHQRRGIQSTSVEDSMSMVHLSVGMKRPASPHLLSEPAIVAGMARAALPDSATPWEWYI
                     EDYDRIRDTMARALDGFEDFNRRVRLPLGFRIKQPARELVFLTPSGRAEFSSAPLPDVV
                     PEPGTLALGTMRSHDQWNTTIYSDNDRYRGIKNLRTLVFMNRADMRERGIADMGPVDIT
                     STAKDGSQRSVSGYLAVPYDIPRGCAAGYMPEMNVLCALVDYSTQSDQPIMKHVKVTIV
                     PSA"
     misc_feature    3769..3784
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.15"
                     /tool="antismash"
     gene            4008..4994
                     /locus_tag="AMK27_RS08565"
                     /old_locus_tag="AMK27_08395"
     CDS             4008..4994
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566630.1"
                     /locus_tag="AMK27_RS08565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08395"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_073904429.1"
                     /transl_table=11
                     /translation="MVVVQPVVAPPSRAAVFLVATVNPGGEDTVREALQDLSGLVRSVS
                     FRHPDDGLTCVAGIGSEAWDRLVGGPRPRELHPFPALRGPRHRAPATPGDLLFHVRARR
                     MDLCFELARLLVESLGTAVTVVDEVHGFAYFDDRDLLGFVDGSENPGGQVAAAAVHIGD
                     EDPGFRGGSYVIVQKYLHEMPAWDALTVEQQEHVIGRGKASNVEMPDDVKPSNSHVALN
                     TIVDEDGVTRQIFRANMPFGRVGGGEFGTYFVGYARTPAVTELMLRNMFLGDPPGNTDR
                     ILDFSRAVTGGLFFVPSAELLDDLPAAPPDAAVRQGDSLGIGGLKGA"
     gene            5001..5867
                     /locus_tag="AMK27_RS08570"
                     /old_locus_tag="AMK27_08400"
     CDS             5001..5867
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037711.1"
                     /locus_tag="AMK27_RS08570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08400"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_241847387.1"
                     /sec_met_domain="Linocin_M18 (E-value: 3e-89, bitscore:
                     294.6, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPDATTPGLTSDPYQGPDTNNLHRELAPITPAAWAEIEEEARR
                     TFRRNVAGRRVVDVPDPGGPDLAAVGTGHLGDLGSPAPGVTARLREAKPLFELRVPFTV
                     DRAAVDDVERGSKDSDWQPVKDAARAMAFVEDRTIFDGFGAAGVDGLRARSSNPAVQLP
                     TEPRDYPDAVSRALTALRLAGVDGPYALLLGADEYRAVSETSDHGYPIAAHLGRMLDGS
                     PIWAPALSGGFVLSTRGGDFELILGQDLAIGYTGHDAESVHLYFQETLTFRTYTDEAVV
                     VLEAPSA"
     gene            6010..6645
                     /locus_tag="AMK27_RS08575"
                     /old_locus_tag="AMK27_08405"
     CDS             6010..6645
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952221.1"
                     /locus_tag="AMK27_RS08575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08405"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_073904431.1"
                     /transl_table=11
                     /translation="MKSPLLLSAVPRLALSALVAAAVGAVVGAATHAPLGVLSAIAVGE
                     TVFVVAGWVTLWPMDATTTHRNVRREEFRPVVEELAVVGTAVAGLVGIVVLLLIGRSDL
                     SHAAAATALAGVFMAWAALHLMYATRYAYIYYLRPGGGIDFNSREPPRYSDFLYFSYNL
                     GMTYQVSDTDVSSTTIRAVALRHCLLSYVFGASILATTINLVTGIVTG"
     gene            6686..8134
                     /locus_tag="AMK27_RS08580"
                     /old_locus_tag="AMK27_08410"
     CDS             6686..8134
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297456.1"
                     /locus_tag="AMK27_RS08580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08410"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_073904432.1"
                     /sec_met_domain="DAO (E-value: 6.9e-09, bitscore: 31.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAVVVGAGPNGLAAAALLAREGLEVTVLEAAEEIGGGTRSVEA
                     ILPGLLHDHCSAIHPMAVTSPALRALGLERHGLRWRQPEIDCVHPLDDGTAGVLLRSVA
                     ETAAGLGADGARYRALLEPSVRRWDALADDVMGPLLRIPAHPLSLARFGLPTALPASAL
                     ARLFRTPQARALWAGVAAHSFRPLSDPFSSAIGLGILTAGHAAGWAVAEGGSHAISRAM
                     AAVLAEHGGVVHTGVRITDRGQLPPADVTLLDLDPGQVGRLYGDRLPPRVAAAYGRFRR
                     GPGAFKVDLAVEGGVPWTDERARRAGTVHLGGPLAEVAFTEREVTEGRMPRRPFVLVGQ
                     QYLADPSRSVGDVHPVWTYAHVPYGYEGDATESVLRQLERFAPGVRERVVGHQVTRPAD
                     FAAANPNFAGGDILTGAKTVPQLLLGARPALDPYATGLPGVFLCSAATPPGPGAHGMCG
                     AGAAASALRHLGARASWQSGRQAG"
     gene            complement(8175..8642)
                     /locus_tag="AMK27_RS08585"
                     /old_locus_tag="AMK27_08415"
     CDS             complement(8175..8642)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037713.1"
                     /locus_tag="AMK27_RS08585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08415"
                     /product="GreA/GreB family elongation factor"
                     /protein_id="WP_073904434.1"
                     /transl_table=11
                     /translation="MTGGPEPISAVAREALERELDDLRTEREAVAVTLEDGGGDQTGDR
                     ADQADELERATELDRLDRRMDDIRGRLREADAAGAPPTDAVGVGSTVTVRFEDGTESTV
                     QIGEVAAVLDRTMVTADSPLGGALLGRRAGDTVTYVTPEGPASAVVLSLGD"
     gene            complement(8688..9971)
                     /locus_tag="AMK27_RS08590"
                     /old_locus_tag="AMK27_08420"
     CDS             complement(8688..9971)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344713.1"
                     /locus_tag="AMK27_RS08590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08420"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_073904436.1"
                     /transl_table=11
                     /translation="MRYSRTCRRRVCAGIAALGLFAAPLGAGTALAAGPSAPGVYPAAQ
                     PPVPGPYSVPAAVSAQGDFPELTPAVTARLDKAIQDVMKETGVPGVTVGLSAPGKGTYI
                     RSFGVADKDTGQPMRPDLYMRIGSETKTFTVTALLQLADRGRLNIDDPISKYVDDVPNG
                     DRITLRNLASMRSGLFNYSADEGFYKAFTSNPDRPFTPQQLLDYAFKHPIQFQPGAEFD
                     YCNTNLILLGLVVEKVSGTPLHEFIQTNVLDPTGLDRTVFPTDAAFPSPHAHGYTVQTA
                     SGQVEDATNWNPSWGWAAGAMISNLQDLRTWARVVATGTLLTPETQAERLKVYPTDIPG
                     AGYGLGIFNVQGWIGHNGSLPGYESLTIYLPEPRATLVVLLNTDELHDGNEPSTLFGQA
                     ITRVVTPEHVFDIPVTAAAPSSPSPSTN"
     gene            10129..10806
                     /locus_tag="AMK27_RS08595"
                     /old_locus_tag="AMK27_08425"
     CDS             10129..10806
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037714.1"
                     /locus_tag="AMK27_RS08595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_08425"
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="WP_073904438.1"
                     /transl_table=11
                     /translation="MAVVHRTTMQPTKLELLTEWLPTREWHAGGEARPERVGGFRLDDP
                     AGEVGIEFMVVTDGEGDGVTAHLVPLTYRGEPLDGAEHALVGTSEHGVLGKRWIYDGTH
                     DPVLVGQLYALLTGRAQAQAQSLSDVPDLTVAVSPQTAEGAEGEADAAGAELVRVEDGP
                     DHTDVVLRTPGAAAPLTLRVRRVLHAGTDLDPGVAGVTAEWRLPDGTSVRGVFAAAVTG
                     LGS"
ORIGIN
        1 ctggcggcgc gcctggagga ggaccgcgag gaggtcggcc ttcccccgga cggcgagcgg
       61 caggccgact agggcccgct aggggtgtcc tgtcgatcag gccgggctcg cggggcccga
      121 tcgacaagac acctcctagt ccacgggcag ccgatccagg gcggctgcca tgtcggtgcg
      181 gctcgcgatc tcgcggggcg tatgcctcac gcggcaggaa cacgcgcgtc aggccaccgg
      241 caccgagccc agtcgctcgt cgcccgtgta cacgttcatc gaggacccgc gcaggaagcc
      301 gaccagggtc agcccgctct ccgccgcgag gtccaccgcg agcgaggacg gcgccgagac
      361 cgccgcgagc agcgggatcc cggccatcac cgccttctgc accagttcga acgaggcgcg
      421 gccgctcacc atcaggaccg actcgcgcag cggcaggagt ccttcgcgca gcgcgtggcc
      481 gacgaccttg tcgacggcgt tgtgccggcc gacgtcctcc cgcaggcaca ccagctcgcc
      541 ctcggcggtg aagagcccgg cggcgtgcag ccctcccgtg gtgtcgaaga cccgctgggc
      601 ggcccgcagc cggtccggca gggccgcgag gacgtccgtc ccgatccgca gcgggtcctc
      661 ggcgaccgac caggcggccg tcgtgcgcac cgcgtccagg ctcgccttgc cgcacagacc
      721 gcacgaggag gtcgtgtaga agttccgctc cagggacgcg tcgggggcgg cgacgccggg
      781 cgccagaccg acgtcgacca cgttgtacgt gttgccgccg tcggccgtgg cccccgcgca
      841 gtaccggatc ccggccacgt cctcggcgga gtgcaccacg ccctcgctga ccaggaagcc
      901 gaccgccagg tcgaagtcgt cgcccggggt ccgcatggtc accgtcagcg ggcgcccgcc
      961 gaccctgatc tccatgggct cctcggcggc cagcgtgtcc gggcggtagg aggagtctcc
     1021 ttcgcgcacg cgcaggacgc gccgccgtac ggtcactcgt cccatggttc gttcctctcc
     1081 tctgagcgcc gctccgacgg tgctcgatgt gcggcccggt cgtccggtcg tccggtcgtg
     1141 cctgttcggg ggctttcccc gactctgtca cggggccgcg gccggcatct ccccgggcgc
     1201 ccgttcgagg cggacgaggg ctgcctacga ggtcgggcag ttgctgatgc cggcgacgct
     1261 gtccagcggc accaggacgc tggtctccgg gcagtacgcc gtggtggagt tgcgggagac
     1321 ggacgacgcg gccgccggtg agctctggcc ggcggagatc gaccccaccg aactcgccgc
     1381 ggcggagcgt ggctacctcc tcctggagca cctcgcccga cggggatgag ggagcgtcac
     1441 gcggagggca cgatcgtcac cttgacgtgc ttcatgatcg gctggtcgct ctgcgtgctg
     1501 tagtcgacca gcgcgcacag cacgttcatc tcgggcatgt agccggccgc gcagccccgg
     1561 gggatgtcgt acggaaccgc gaggtagccg ctgaccgacc gctgggagcc gtccttcgcg
     1621 gtgctcgtga tgtcgacggg gcccatgtcg gcgatcccgc gctcccgcat gtcggcccgg
     1681 ttcatgaaga ccagcgtgcg caggttcttg atgccgcggt agcggtcgtt gtcggagtag
     1741 atggtggtgt tccactggtc gtgggaccgc atggtgccga gggccagggt gcccggctcg
     1801 ggtacgacgt cgggcagcgg ggcgctggag aactcggcgc gcccggacgg ggtgaggaag
     1861 accagttcgc gggccggctg cttgatgcgg aagccgagcg ggaggcgtac ccgccggttg
     1921 aagtcctcga agccgtcgag ggccctggcc atggtgtccc ggatgcggtc gtagtcctcg
     1981 atgtaccact cccagggcgt cgcgctgtcg ggcagcgccg cccgcgccat gccggcgacg
     2041 atcgccggtt cggagagcag gtgcggggaa gccgggcgct tcatgccgac cgacaggtgg
     2101 accatgctca tcgagtcctc gacggaggtg ctctggatgc ccctgcgctg gtggtccttc
     2161 tcggtgcggc ccaggcacgg caggatcagt gcctgcttgc cgtggacgag gtggctgcgg
     2221 ttcagcttgg tgctgacctg gacggtgagg tcgcaggtgc gcagcgccgc gtaggtgagc
     2281 ggggtgtcgg gggcggcgag cgcgaagttc ccgcccatgc cgacgaagac cttgatctcg
     2341 ccgcgctgca tcgcctcgat ggtgcggacg gtgtccaggc cgtgctcgcg cggcggttcg
     2401 atctcgcaga cctcggcgag gcggtcgagg aatgcctcgc tcgggcggtg gtcgatgccg
     2461 caggtgcggt tgccctggac gttgctgtgg ccgcgtacgg gggaggggcc ggcgccctcg
     2521 cgccccaggt tgccgcggag cagcagcagg ttgacgatct cccggacggt gtcgacgccg
     2581 tgctcgtgct gggtgaggcc cagacaccag ctgacgatgg agcggtcggc ctcgccgtac
     2641 acccgcgcgg ccttgaggat gtcggcgcgg gacagtccgg actgccgctc gatctcctcc
     2701 cacggtgtgg cctcgcacag cgcccggtac tcctcgaagc cgctcgtgtg gcggtcgacg
     2761 aactcctggt cgagggcctt ggggtcggtc gccgactgct ccaggaccgc cttggccatg
     2821 ccgcggatca gcgccatgtc gccgccgatg cgcggctgga ggttcagggt gctggtcttc
     2881 gtcgtcttga agagggccat gtccgtgaag tcgtgcggca cgatggtccg ggtggcggct
     2941 gcctcgacga gcgggttgac gtgcacgatc tgcgcgccgc ggcggtgcgc ctcggcgagc
     3001 gcggtcagca tcctgggggc gttcgacgcg gcgttcaccc cgaagatgaa cagcgcgtcc
     3061 gcggtctccc agtccttcag gtcgacggtg cccttgcccg tgcccaggga ggcctgaagg
     3121 gcgcggccgc tggcctcgtg gcacatgttg gagcagtcgg gcaggttgtt ggtgcccagc
     3181 tcgcgggcca tcagctggta gaggaaggtg gcctcgttgc cgagccgtcc cgaggtgtag
     3241 aaggacgcct ggtgggggtt gtcgaggccg cgcagggcac ggccgaccac ctcgaacgcg
     3301 tccttccacg tgatcgggac gtagtggtcc gactccgggt cgtagaccat cggctcggtc
     3361 agccggccct cgttctccag gtcgtagtcg ctccactcgg ccagctcgga caccgagtgc
     3421 gcggcgaaga actcgccgcc gacgcgcttg cgcgtcatct cccaggtgac gtgcttgatg
     3481 ccgttctcgc agatgtccag gtgcaggccc ttggtgtcgt cgggccacgc gcagccgggg
     3541 cagtcgaatc cggtgttctc gtggttcatc cgcatgatgg cgcgcgggcc gtcgacgagg
     3601 gcgccctcgc ggacgaggaa gccggtcacg ctcctggccg cgccccagcc cgccgccggg
     3661 tggtggtagg ggtggaactc cgggtcgccc tcgggggtgg gccccacccg ggcctccacg
     3721 ggttccgggg cgccgggcgc cggggattcc tgcgcgtcct ggtcggtgct cacggcgttg
     3781 tcaccctttc acctgcggat gttcggggca cagccgaaca gaaatcaccc ctttgggcag
     3841 gctatgcccg ccggatcgcg ggtgccactc ggctcgatcg cgggcgccgc tcggatccga
     3901 ggggccgcac gggctgcgta ccggatcatg ggcgcctacg ctggcgctgt ccttttcatg
     3961 tcttttgtct gtcagtcttt tgtctgccat ctctcggcgg gagtcccatg gtcgtcgtcc
     4021 aacccgtcgt cgccccgccc tcccgggctg ccgtgttcct cgtggccacc gtcaaccccg
     4081 gcggcgagga caccgtgcgg gaggcgctcc aggacctgtc aggactcgtc cggtcggtgt
     4141 ccttccgcca ccccgacgac ggcctcacct gcgtcgccgg gatcggctcc gaggcctggg
     4201 accggctcgt cggcggtccc cgccctcgcg agctgcaccc cttcccggcc ctcagaggcc
     4261 cccgccaccg ggccccggcc accccgggcg acctgctgtt ccacgtacgc gcccggcgca
     4321 tggacctctg cttcgagctc gcccggctcc tcgtggagag cctcggcacc gccgtgaccg
     4381 tcgtcgacga ggtgcacggt ttcgcgtact tcgacgaccg cgacctcctc ggcttcgtcg
     4441 acggcagcga gaacccggga ggccaggtgg cggccgccgc ggtccacatc ggcgacgagg
     4501 accccggctt ccgcggcggc agctacgtca tcgtgcagaa gtacctgcac gagatgcccg
     4561 cctgggacgc gctcaccgtc gaacagcagg agcacgtcat cggacgaggc aaggcgtcca
     4621 acgtggagat gcccgacgac gtcaagccgt cgaactcgca cgtcgcgctg aacaccatcg
     4681 tcgacgagga cggcgtcacc cggcagatct tccgcgcgaa catgccgttc ggccgggtcg
     4741 gcggcggcga gttcggcacc tacttcgtcg gctacgcccg cacgcccgcc gtcaccgagc
     4801 tgatgctgcg caacatgttc ctcggagatc ccccgggcaa cacggaccgc atcctcgact
     4861 tctcccgcgc cgtgaccggc ggcctcttct tcgtgccgag cgccgaactc ctcgacgacc
     4921 tgcctgccgc gccgccggac gccgccgtcc gccagggaga ctccctgggc atcggcggcc
     4981 tgaaaggagc ctgactcccc atgacgaccc ccgacgccac gacccccggc ctcacgagcg
     5041 acccctacca gggtcccgac acgaacaacc tgcaccgcga actcgccccg atcaccccgg
     5101 cggcctgggc cgagatcgag gaggaggccc gccgcacgtt ccgccgcaac gtcgcgggcc
     5161 gccgcgtcgt cgacgtgccc gaccccggag gccctgacct cgccgccgtc ggcaccgggc
     5221 acctcggcga cctgggctcg cccgcccccg gtgtcaccgc gcggctgcgc gaggcgaagc
     5281 cgctgttcga actcagggtg cccttcaccg tcgaccgggc cgccgtcgac gacgtcgagc
     5341 gcggctcgaa ggactccgac tggcagcccg tcaaggacgc ggcgcgcgcc atggcgttcg
     5401 tcgaggaccg cacgatcttc gacggattcg gggcggccgg tgtcgacggc ctgcgcgcca
     5461 ggtcctccaa cccggccgtc cagctcccca ccgaaccgcg cgactacccc gacgccgtca
     5521 gccgggccct gaccgcgctg cgcctcgccg gcgtcgacgg cccctacgcg ctgctcctcg
     5581 gcgccgacga gtaccgggcg gtcagcgaga cctccgacca cggctatccg atcgccgccc
     5641 acctcggccg catgctcgac ggctcgccga tctgggcgcc ggccctgagc ggcggtttcg
     5701 tcctgtccac ccgcggcggc gacttcgagc tgatcctcgg ccaggacctg gcgatcggct
     5761 acacgggcca cgacgcggag agcgtgcacc tgtacttcca ggagacgctc accttccgca
     5821 cgtacaccga cgaggccgtg gtcgtcctgg aggcaccgag cgcctgaggg cgtcccacct
     5881 ggccgtcacg acagccctgg gggacacgag agccctgcgg gacacgacag cccctggggg
     5941 acacgacagg cctgggggac acgacagccc tgcgggaacg gaggcccgag agccgggagg
     6001 cccgatgcca tgaagagtcc gctgctcctc tccgccgtgc cccggctcgc cctctcggcg
     6061 ctggtcgcgg cggcggtcgg cgccgtcgtc ggcgccgcca cccacgcgcc gctgggcgtc
     6121 ctctcggcga tcgccgtcgg ggagacggtc ttcgtcgtcg caggctgggt cacgctgtgg
     6181 ccgatggacg ccaccaccac ccaccgcaac gtacgccgcg aggagttccg gcccgtcgtc
     6241 gaggaactgg ccgtcgtcgg aacggcggtg gccggactcg tcggcatcgt cgtcctcctg
     6301 ctgatcggca ggtccgacct cagccacgcc gccgcggcga ccgcgctcgc cggggtgttc
     6361 atggcctggg ccgcgctgca cctgatgtac gccacccggt acgcgtacat ctactacctg
     6421 agacccggcg gcgggatcga cttcaactcc cgcgaaccgc cccgctacag cgacttcctc
     6481 tacttcagct acaacctcgg catgacctac caggtctccg acaccgacgt ctccagcacg
     6541 acgatccgcg cggtcgcact ccgtcactgc ctcctgtcgt acgtcttcgg cgcgagtatc
     6601 ctggccacca ccatcaacct cgtcacgggc atcgtcacgg gctgaggcgg ttcgcacgga
     6661 gactcggccc cctggagacc acgtcatgac cacggccgtc gtcgtcggtg ccggacccaa
     6721 cgggctcgcc gccgccgccc tgctcgcgcg cgaaggcctg gaggtcaccg ttctggaggc
     6781 cgccgaggag atcggcggcg gcacccgcag cgtcgaggcg atcctgcccg gtctcctgca
     6841 cgaccactgc tccgccatcc atccgatggc cgtcacctcg cccgcgctcc gcgcgctcgg
     6901 cctggagcgc cacgggctgc gctggcggca gcccgagatc gactgcgtgc acccgctcga
     6961 cgacggcacg gccggggtcc tgctccggtc cgtcgcggag accgccgccg ggctcggtgc
     7021 cgacggcgcc cgctaccggg ccctgctgga gccgtccgta cggcggtggg acgcgctcgc
     7081 cgacgacgtc atgggcccgc tcctccggat ccccgcgcat ccgctgtccc tggcccgctt
     7141 cggcctgccg accgccctgc ccgcctccgc cctcgcccgg ctcttccgca cgccgcaggc
     7201 gcgggccctg tgggcgggag tcgcggccca ctccttccgc ccgctctccg acccgttcag
     7261 ctcggccatc gggctcggca tcctcaccgc cggacacgcc gccgggtggg ccgtcgccga
     7321 gggcggttcg cacgccatct cgcgcgccat ggccgccgtc ctcgccgaac acggcggagt
     7381 ggtccacacc ggtgtgcgga tcaccgaccg cgggcagctc ccgcccgccg acgtgaccct
     7441 cctcgacctg gaccccggcc aggtcgggcg actgtacggc gaccggctgc caccccgggt
     7501 ggcggccgcg tacgggcgct tccggcgcgg ccccggcgcc ttcaaggtgg acctcgccgt
     7561 cgagggcggg gtgccctgga cggacgagcg ggcccggcgc gccggcaccg tgcacctcgg
     7621 cggcccgctc gccgaagtgg ccttcaccga gagggaggtg acggagggtc ggatgccccg
     7681 gcggcccttc gtgctcgtcg gacagcagta cctcgccgac ccctcccggt ccgtcggcga
     7741 cgtccacccc gtctggacgt acgcgcacgt gccgtacggc tacgaaggcg acgcgaccga
     7801 gtcggtcctg cggcagctgg aacgcttcgc gcccggcgtc cgcgagcggg tcgtgggcca
     7861 ccaggtcacc cgaccggccg acttcgccgc cgccaacccc aacttcgccg gcggggacat
     7921 cctcaccgga gccaagaccg tcccccagct cctcctgggc gcacgccccg cgctcgaccc
     7981 ctacgccacc gggctgccgg gcgtgttcct ctgctccgcc gccaccccgc ccggccccgg
     8041 agcccacggc atgtgcggcg cgggagcggc ggcgtcggca ctgcgccacc tcggggcacg
     8101 ggcctcgtgg cagagcggcc gacaggccgg ctagtccgcg gagccggccc cgcgagtccc
     8161 tgtgaggaca ccctctagtc gccgagggac agcacgaccg cggaggccgg cccctccggg
     8221 gtcacgtacg tgacggtgtc accggcgcga cggcccagca gcgcgccgcc gagggggctg
     8281 tccgcggtga ccatcgtccg gtcgagcacc gcggcgacct cgccgatctg gacggtcgac
     8341 tccgtaccgt cctcgaaccg cacggtcacc gtgctgccca cgccgaccgc gtcggtgggc
     8401 ggcgcgcccg cggcgtccgc ctcgcgcagg cgtccccgga tgtcgtccat gcggcggtcg
     8461 agccggtcga gttccgtggc gcgctccagt tcgtcggcct ggtcggcccg gtcgcccgtc
     8521 tgatcgccgc ctccgtcctc gagcgtgacg gcgacggcct cgcgttcggt gcggaggtcg
     8581 tcgagttccc gctccagtgc ctcgcgggcg acggcgctga tgggctcggg tccaccggtc
     8641 attcctgctc ccgtggtgcg gcggcacggg tggtgtgccg cgggcggtca gttggtgctg
     8701 gggctcgggc tgctcggagc cgccgccgtg accgggatgt cgaagacgtg ctcgggggtc
     8761 acgacgcggg tgatggcctg tccgaacagg gtgctgggct cgttgccgtc gtgcagctcg
     8821 tccgtgttga ggagcacgac cagggtggcc cgcggctccg gcaggtagat cgtcagcgac
     8881 tcgtacccgg gcagggatcc gttgtggccg atccagccct ggacgttgaa gatgccgagg
     8941 ccgtatccgg caccggggat gtccgtcgga tagaccttga ggcgctcggc ctgcgtctcc
     9001 ggggtgagca gcgtgccggt ggcgaccacc cgtgcccagg tgcgcaggtc ctggaggttc
     9061 gagatcatcg cgccggccgc ccagccccag gaggggttcc agttcgtggc gtcctcgacc
     9121 tggcccgagg cggtctggac ggtgtagccg tgggcgtgcg gggacgggaa cgcggcgtcg
     9181 gtcgggaaga cggtccggtc caggcccgtc gggtcgagaa cgttcgtctg gatgaactcg
     9241 tgcagcggtg tgccgctgac cttctccacg acgagtccga gcaggatcag gttggtgttg
     9301 cagtagtcga actcggcacc gggctggaac tggatcgggt gcttgaaggc gtagtcgagc
     9361 aactgctgcg gggtgaaggg ccggtcgggg ttcgaggtga acgccttgta gaagccctcg
     9421 tccgccgagt agttgaagag cccgctgcgc atggacgcga ggttgcgcag cgtgatcctg
     9481 tcgccgttcg gtacgtcgtc gacgtacttc gagatcgggt cgtcgatgtt cagccggccc
     9541 cggtcggcga gttggagcag cgcggtgacc gtgaacgtct tggtctcgct gccgatccgc
     9601 atgtacaggt cgggccgcat cggctgcccg gtgtccttgt cggcgacgcc gaaggagcgg
     9661 atgtacgtgc ccttgccggg cgccgagagg ccgaccgtga caccggggac gccggtctcc
     9721 ttcatgacgt cctggatcgc cttgtccagg cgtgcggtga cggccggggt gagttcgggg
     9781 aagtcgccct gcgccgagac cgccgcgggg accgagtagg gtccgggaac cggtggttgg
     9841 gccgccgggt acacgccggg ggcggacggt ccggcggcga gggcggtgcc cgcgcccagc
     9901 ggggcggcga agagtccgag agccgcgatg cccgcgcaga cgcgcctgcg gcatgtgcgt
     9961 gagtacctca cggcggcctc ctgccggccg gggacaacac tccactccca ggctaggccg
    10021 ggccgccggg cctcgcacgg cggctccgcc gcgtggtctg ttacgttgcc gatcaccggg
    10081 ccgcggagca gaggggcccg gcccgcgagc gagagggaga cccgcgccat ggccgtcgtc
    10141 caccggacca ccatgcagcc gaccaagctg gaactgctca ccgagtggct ccccacccgg
    10201 gagtggcacg cgggcgggga ggcgcggccg gagcgcgtcg gcgggttccg gctcgacgac
    10261 ccggcgggcg aggtcggcat cgagttcatg gtggtgaccg acggagaggg cgacggggtt
    10321 accgcccacc tggtccccct gacgtaccgc ggggagccgc tggacggcgc cgagcacgcc
    10381 ctcgtgggca cgtccgagca cggcgtcctc ggcaagcgct ggatctacga cggaacccac
    10441 gacccggtgc tcgtcggcca gttgtacgcg ctcctcaccg gccgcgcgca ggcccaggcc
    10501 cagtccctgt ccgacgtccc ggacctcacc gtcgccgtct cgccgcagac ggcggagggc
    10561 gcggagggcg aggcggacgc ggccggggcg gagctcgtac gggtcgagga cggcccggac
    10621 cacacggacg tggtgctgcg cacccccggc gcggccgccc cgctgaccct gcgcgtacgg
    10681 agggtcctcc acgccggcac ggacctggac ccgggtgtcg ccggagtgac cgccgagtgg
    10741 cggctccccg acggaacctc cgtccggggc gtgttcgccg ccgcggtcac cggtctcggg
    10801 tcctagcgcg cgagcccggc cgctcgacac ggctcccctg gatccccggt cggttagacc
    10861 cagttga
//
