LOCUS       NZ_LIVV01000001        10417 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02009 scaffold1, whole genome shotgun sequence.
ACCESSION   NZ_LIVV01000001
VERSION     NZ_LIVV01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02009
  ORGANISM  Streptomyces sp. CB02009
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1660693)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660693)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905825.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,214
            CDSs (total)                      :: 8,139
            Genes (coding)                    :: 7,984
            CDSs (with protein)               :: 7,984
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 51 of 155
            Pseudo Genes (incomplete)         :: 117 of 155
            Pseudo Genes (internal stop)      :: 15 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:06:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 769386
            Orig. end                         :: 779803
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVV01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(570..1295)
                     /locus_tag="AMK27_RS03405"
                     /old_locus_tag="AMK27_03350"
     CDS             complement(570..1295)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032245.1"
                     /locus_tag="AMK27_RS03405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03350"
                     /product="flavodoxin family protein"
                     /protein_id="WP_073902507.1"
                     /transl_table=11
                     /translation="MAVPDSTPYRYDDLGALYVNCTLKRSPEVSNTEGLIERSRSVMEA
                     QGVATSLVRAVDLDIATGVWPDMREHGWESDAWPALYEQVMAADILVLCGPIWLGDNSS
                     VMKRVIERLYACSSLLNGAGQYAYYGRAGGCLITGNEDGVKHCAMNVLYSLQHLGYTVP
                     PQADAGWIGPAGPGPSYLDPGSGGPENDFTNRNTTFMAWNLMHVAGLLKRAGGIPAHGN
                     QRSAWDAGCRPDFPNPEHR"
     gene            complement(1424..2227)
                     /locus_tag="AMK27_RS03410"
                     /old_locus_tag="AMK27_03355"
     CDS             complement(1424..2227)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031440.1"
                     /locus_tag="AMK27_RS03410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03355"
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="WP_073902508.1"
                     /transl_table=11
                     /translation="MTSRSYDVLAGAPDSPVLLHVPHSSRVIPPDVREGILLADGELGL
                     ELDHITDAHTAEIAAAAAAAAGATPWQFVNRLSRLVVDPERFPDEREEMLAVGMGAVYT
                     RTTHRAPLRPAGFDPAPLIDRYFTPYAEGMAAAVAERLEATGRAVIVDVHSYPTGPLPY
                     ELHGEGPRPPVCLGTDAFHTPPALLDAAREAFGGFGGLATDTPFAGTYVPLSYYGRDPR
                     VSALMIEIRRDVYMSEPGGAAGPGVQALGAALAALVDAVPPHPAA"
     gene            complement(2286..2738)
                     /locus_tag="AMK27_RS03415"
                     /old_locus_tag="AMK27_03360"
     CDS             complement(2286..2738)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019979912.1"
                     /locus_tag="AMK27_RS03415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03360"
                     /product="ATP-binding protein"
                     /protein_id="WP_107425464.1"
                     /transl_table=11
                     /translation="MKTWSDTRHPAPGADPRGARSAGAPPVRTPADARQLVRHTLEALG
                     PLRPAQVEDLLLVTSELVTNAHRHAGGVTGFGIGVGHDQVTVSVSDASGQTPLYERPAD
                     LRPGGYGWPIVLRLCREVTVDAGPDGKTIHAAVAVERCAVPEPAGT"
     gene            2996..3835
                     /locus_tag="AMK27_RS03420"
                     /old_locus_tag="AMK27_03365"
     CDS             2996..3835
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140318.1"
                     /locus_tag="AMK27_RS03420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03365"
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="WP_073903883.1"
                     /transl_table=11
                     /translation="MGGELPFPEVPHAVPPRDARALSKVFLRELAAREEGTAEHQYVRN
                     TLIEMNMSLVVYAAGRFRSRGDEREDILQVGMIGLIKAIDRFDLSREVEFATFAVPYIV
                     GEIKRFFRDSTWAVHVPRRLQEARVELAKATDELSARLGRAPRPSELAPVMDLSEDEVV
                     EAQVAANGYHSTSLDARLAGDSEEEDAALADLIGQEDPALALFEEFHTLAPLVEALAPR
                     DRLLLHLRFVEELTQTEIGERIGVSQMQVSRLLARSLDRLRDGMVEDAGEGREECAG"
     gene            complement(4032..4928)
                     /gene="thpD"
                     /locus_tag="AMK27_RS03425"
                     /old_locus_tag="AMK27_03370"
     CDS             complement(4032..4928)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536874.1"
                     /locus_tag="AMK27_RS03425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03370"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073902509.1"
                     /sec_met_domain="PhyH (E-value: 1.5e-35, bitscore: 119.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSAPTRPVDLYPTRGPKEELIGRKDPVVWSGPGTAGPFWARELE
                     EFERDGFATVDEIVTPDEVDVLRAELDRLVSDPAVRADERAVVEPRSQEIRSVFEVHRM
                     SEVFGRLARDPRIVGRARQILGSDVYVHQSRVNVKPGFGASGFYWHSDFETWHAEDGLP
                     RMRTVSVSIALTPNHTTNGSLMIMPGSHRTFLGCAGATPKDNYKRSLQMQDAGTPSDEA
                     LTTFAEACGIRHFTGPAGSATWFDCNALHGSGDNITPYPRSNVFLVFNSVENQPEAPFA
                     APVRRPSYIAAPFSDHV"
     gene            complement(5001..5417)
                     /locus_tag="AMK27_RS03430"
                     /old_locus_tag="AMK27_03375"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031435.1"
                     /locus_tag="AMK27_RS03430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03375"
                     /product="ectoine synthase"
                     /protein_id="WP_073902510.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.3e-52, bitscore:
                     173.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTFDELENTERHVKAASGTWESKRIVLARERVGFSLHETVLY
                     AGTETAMWYANHVEAVVCTAGEAELTDHETGRTYTIVPGTMYLLDGHERHTLKVKADFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEPASDDA"
     gene            complement(5491..6762)
                     /gene="ectB"
                     /locus_tag="AMK27_RS03435"
                     /old_locus_tag="AMK27_03380"
     CDS             complement(5491..6762)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 6.4e-73.
                     Score: 237.6. Matches aSDomain:
                     nrpspksdomains_AMK27_RS03435_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="AMK27_RS03435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03380"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073902511.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.7e-77, bitscore:
                     257.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDIATPSVFETVESEVRSYCRAWPVVFERAVGSRLYDEQGRP
                     YLDFFAGAGSLNYGHNNPVLKRALLEYLGNDGITHGLDMSTTAKRDFLEAFHTTVLEPR
                     ALPYKVMFPGPTGTNAVEAALKLARKVTGRQTVVSFTNAFHGMSLGSLAVTGNAAKRAG
                     AGVPLPHAIRMPFDDYLDGQLPDFLWFERLLNDAGSGLDHPAAVIVETVQGEGGINVAR
                     AEWLRALADLCRRHEMLLIVDDIQMGCGRTGDFFSFEEAGITPDIVTLSKSISGYGLPM
                     ALCLFRPELDVWQPGEHNGTFRGNNPAFVTATAALDAYWRDGTLRGRTLGRADRVERAL
                     LDLCGGDARTGLSVRGRGLVWGLEFADGERAEAVCRRAFETGLLVETSGPSGEVVKLLP
                     PLTATDDELDEGLGILARAVRHTA"
     aSDomain        complement(5665..6675)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK27_RS03435_Aminotran_3.1"
                     /evalue="6.40E-73"
                     /label="AMK27_RS03435_Aminotran_3.1"
                     /locus_tag="AMK27_RS03435"
                     /protein_end="366"
                     /protein_start="29"
                     /score="237.6"
                     /tool="antismash"
                     /translation="FERAVGSRLYDEQGRPYLDFFAGAGSLNYGHNNPVLKRALLEYLG
                     NDGITHGLDMSTTAKRDFLEAFHTTVLEPRALPYKVMFPGPTGTNAVEAALKLARKVTG
                     RQTVVSFTNAFHGMSLGSLAVTGNAAKRAGAGVPLPHAIRMPFDDYLDGQLPDFLWFER
                     LLNDAGSGLDHPAAVIVETVQGEGGINVARAEWLRALADLCRRHEMLLIVDDIQMGCGR
                     TGDFFSFEEAGITPDIVTLSKSISGYGLPMALCLFRPELDVWQPGEHNGTFRGNNPAFV
                     TATAALDAYWRDGTLRGRTLGRADRVERALLDLCGGDARTGLSVRGRGLVWGLEFA"
     gene            complement(6815..7345)
                     /gene="ectA"
                     /locus_tag="AMK27_RS03440"
                     /old_locus_tag="AMK27_03385"
     CDS             complement(6815..7345)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031433.1"
                     /locus_tag="AMK27_RS03440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03385"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_073902512.1"
                     /transl_table=11
                     /translation="MTPLTTGLTTTTLRTTPPTLADGAELWRIARGSGELDLNSPYSYL
                     LWCRDFAGTTTVARDASGRPIGFVTGYLRPDEPETLFVWQIAVEGSHRGSGVAGTLLDA
                     LSAQVAAEHGISRIEATVTPGNLASDRLFRSFARRHDAEVTQEVLFPASSFPAAGHESE
                     VLYHIGPLGPPAS"
     misc_feature    7453..7463
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    7578..7587
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(7710..8513)
                     /locus_tag="AMK27_RS03445"
                     /old_locus_tag="AMK27_03390"
     CDS             complement(7710..8513)
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031432.1"
                     /locus_tag="AMK27_RS03445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03390"
                     /product="endonuclease"
                     /protein_id="WP_073902513.1"
                     /transl_table=11
                     /translation="MSVVRIGSWKVWAAAAAATLVGVTLPTVAATPAGATTTAYDSTYY
                     KNAIGKTGTSLKSSLHTIISSQSKISYDAVWNALKTTDQDPANTNNVILLYSGTSRSKS
                     LNGGDVGDWNREHTWAQSHGNFGTSAGPGTDLHHLRAADVQVNSIRGNKDFDNGGSAVS
                     GAPGSYTDSNSFEPRDADKGDVARMILYMAVRYEGDDAWADLEPNESTTNGSVRFHGRL
                     SVLKQWNEQDPPSAFEERRNDIIHTTYQGNRNPFIDHPEWVEAIW"
     misc_feature    complement(8576..8591)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            complement(8748..9212)
                     /locus_tag="AMK27_RS03450"
                     /old_locus_tag="AMK27_03395"
     CDS             complement(8748..9212)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031431.1"
                     /locus_tag="AMK27_RS03450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK27_03395"
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_073902514.1"
                     /transl_table=11
                     /translation="MDRIDLHILRELQSDGRLSNQELAQRVGLSPSPCLRRVRQLEQDG
                     VIQGYRAIIDPEAVGRGFEVLVSVEVRRDRETVEAFEEALRDVPDVIEAYRLFGSPGCL
                     LRIAVADLAAYERLWIERLTTLTGVTEVNSQIIMKRIKEPKGMPVDVRGG"
     misc_feature    complement(9198..9211)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(10130..10144)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 gcacgatccg ccgggtggcg tcaccgccct ccaggtcctc ggccgctccg agcagcgccg
       61 gaccgatcgg cgtccagccg gtcggggcca gggtggccac ggcggtcttg gcctcggtgc
      121 ggtcgagcgg gcccaccgga tagagctgcc gggtgtcctt gcagccccgc ttgcggtcct
      181 gccccgggta gtcggcgccc agggtgcgga tgccgagctg gacctccgag ggcaccgcgt
      241 cgaggacctc gttgaaggcc tgcttggccg cggccatccg ggacttgccg tcgatgtcct
      301 tggcccgcat ggagccgctc acgtcgagga ccagctcgac cttgggggat tccttcgcca
      361 ccggcgcgtc cgcggtcgcg cccgtcggca ggagcgcgac gctcagggcg gcgagcagcg
      421 cgcaggcccc ggtcgccagc cgttttctcg tgatcatcgc cggatcgtat tgatgatcct
      481 ccgacaatcc aaaacggggg tgtgagccgg ggcctgagat gcggtcagaa cgggggagac
      541 cgcgcggctc gccgagcggc ccccgggtgt cagcggtgct cggggttcgg gaagtccggc
      601 ctgcagcccg cgtcccacgc cgagcgctgg ttgccgtggg ccgggatgcc gcccgcccgc
      661 ttgagcagcc cggcgacatg catcaggttc caggccatga acgtggtgtt gcggttggtg
      721 aagtcgttct cggggccgcc cgagcccggg tccaggtagg aggggccggg acccgccggg
      781 ccgatccagc ccgcgtcggc ctgcggcggc accgtgtacc ccaggtgctg gaggctgtag
      841 aggacgttca tcgcacagtg cttcacgccg tcctcgttgc cggtgatcag acagccgccg
      901 gcccggccgt agtacgcgta ctgcccggca ccgttgagga ggctcgagca ggcgtagagc
      961 cgctcgatca cccgcttcat cacggagctg ttgtcgccga gccagatcgg cccgcagagc
     1021 acgaggatgt cggcggccat cacctgctcg tacagcgcgg gccaggcgtc cgactcccag
     1081 ccgtgctccc tcatgtccgg ccacaccccg gtcgcgatgt ccaggtcgac ggcccgcacc
     1141 agggaggtcg ccacgccctg cgcctccatg acggaccggc tgcgctcgat cagcccctcg
     1201 gtgttgctga cctcgggcga gcgcttgagc gtgcagttga cgtacagcgc gccgaggtcg
     1261 tcgtaccggt acggggtgga atcgggcacg gccatcgacg ggtctcctca cgcacggggg
     1321 gcgatccctc cagggaccac ccccagcctg cgcacggccc ccgccccgga gccacccggc
     1381 agcgaccgaa cgcatgagca ggcgcccgga ggcgtgaccg gccctacgcc gccgggtgcg
     1441 gcggcacggc gtcgacgagc gccgccaggg cggccccgag cgcctggacc cccggtcccg
     1501 ccgcgccgcc cggctcgctc atgtagacat cgcgccgaat ctcgatcatg agcgcgctga
     1561 cccgcggatc cctgccgtag taggacagcg gtacgtacgt cccggcgaag ggcgtgtccg
     1621 tcgccaggcc cccgaacccg ccgaacgcct cccgggcggc gtccagcagg gccggcggcg
     1681 tgtggaaggc gtcggtaccg aggcagaccg gcgggcgggg gccctcgccg tggagctcgt
     1741 acggcagcgg cccggtcggg tacgagtgga cgtcgacgat cacggcccgc cccgtcgcct
     1801 ccaggcgttc cgccacggcg gcggccatgc cctcggcgta cggcgtgaag taccggtcga
     1861 tcagcggcgc cgggtcgaat ccggcgggac gcagcggcgc ccggtgcgtg gtcctcgtgt
     1921 acaccgcgcc catcccgacg gccagcatct cctcgcgctc gtcggggaac cgctccgggt
     1981 cgacgaccag ccgggacagc cggttgacga actgccaggg ggtggcgccc gcggccgccg
     2041 cggccgcggc cgcgatctcc gccgtgtggg cgtcggtgat gtggtcgagc tcgaggccca
     2101 gttccccgtc cgccagcagg atgccctcgc gcacgtccgg gggtatcacc cgggaggagt
     2161 gcgggacatg gagcagtacg ggggagtccg gggcgccggc gaggacgtcg tacgagcgtg
     2221 aggtcatcgg ctcagccttc cacagcgcgg gcctcgcggg tcatcgggtc acgaaggtca
     2281 ccggctcacg ttccggcggg ctccggaacg gcgcaccgct cgaccgccac ggccgcgtgg
     2341 atggtcttgc cgtcgggtcc cgcgtcgacg gtcacctcgc ggcacaggcg gagcacgatc
     2401 ggccagccgt agcccccggg acgcagatcc gcggggcgct cgtacagcgg cgtctgcccg
     2461 ctggcgtcgg acaccgacac cgtcacctgg tcgtgtccga ccccgatccc gaatccggtg
     2521 acgccgcccg cgtgccggtg cgcgttcgtg accagctccg aggtgacgag cagcaggtcc
     2581 tcgacctgcg cgggtcgcag ggggccgagc gcctcgaggg tgtggcggac gagctgccgc
     2641 gcgtcggccg gagtacggac cgggggcgca ccggcactcc gggcaccgcg cgggtccgcc
     2701 ccgggggccg ggtggcgggt gtcgctccag gtcttcatcg cgcatcccct tcttcctgtt
     2761 tcttcctgct tcttgatgtt tcttactgtt tcctcaggag gtgatgggtt tcgtcttccc
     2821 gttcgaaccg gggtgatgcc cattgccggg cggaatcagc cgcgagcgga cagggaatgc
     2881 gtccgaaaag cgggggtacg aggacaagga caactgaacg gaccgcgagt cggaggaggt
     2941 tgttcctgtg cggaccgatc aggccgaggc gacgacggcg tacgcgacga acggcatggg
     3001 cggggaactg ccctttcccg aggtcccgca cgccgttcca ccgcgggacg cgcgtgccct
     3061 gtcgaaggtg ttcctccgcg aactcgcggc gcgggaggag ggcaccgcgg agcaccagta
     3121 cgtccggaac accctcatcg agatgaacat gtccctggtg gtgtacgcgg cggggcggtt
     3181 ccggtcgcgc ggggacgagc gggaggacat cctgcaggtc ggcatgatcg gcctgatcaa
     3241 ggcgatcgac cgcttcgacc tgtcccgcga ggtggagttc gccaccttcg ccgtgccgta
     3301 catcgtgggt gagatcaaac ggttcttccg cgacagcacc tgggccgtgc acgtgccgcg
     3361 ccgcctccag gaggcgcggg tggagctggc caaggcgacc gacgagctga gcgcgcgcct
     3421 cggccgggca ccccgcccct ccgagctggc gccggtcatg gacctgtccg aggacgaggt
     3481 ggtcgaggcc caggtcgccg ccaacggcta ccactccacg tccctggacg ccaggctcgc
     3541 cggcgacagc gaggaggagg acgcggccct ggccgatctc atcggccagg aggacccggc
     3601 gctcgccctc ttcgaggagt tccacaccct cgccccgctc gtggaggccc tcgccccgcg
     3661 cgatcggctg ctgctgcacc tccgcttcgt ggaggagctc acccagacgg agatcgggga
     3721 gcggatcggc gtctcccaga tgcaggtctc acgtctgctc gcccgaagcc tggaccgcct
     3781 gagggacggc atggtcgagg acgccggtga gggccgggag gagtgcgcgg gctgagcggc
     3841 ccgaggaggg tgctcgtgcg atgcgtgccc ggtgagtgcg cggtgacact gcagcgagga
     3901 acgcgcggcc cgggatgccc gggccgcgcg ttcctgtgtg cgggccgatc ggaagccgcg
     3961 gggtcagcgc ggggcctccg aggagcgccg ttccgccgag gaggcctcgt cgggacgggc
     4021 gcggcgcgcc ctcacacgtg atctgagaag ggcgccgcga tgtacgaggg ccgccggacc
     4081 ggggcggcga agggcgcctc cggctggttc tcgacgctgt tgaagacgag gaacacattg
     4141 ctccgggggt acggggtgat gttgtcgccc gacccgtgca gggcgttgca gtcgaaccag
     4201 gtggcggagc ccgccgggcc cgtgaagtgg cggatgccgc aggcctcggc gaacgtggtc
     4261 agcgcctcgt cggacggcgt gccggcgtcc tgcatctgca gcgagcgctt gtagttgtcc
     4321 ttgggggtgg caccggcgca gcccaggaac gtgcggtgcg atcccggcat gatcatcagg
     4381 ctgccgttgg tggtgtggtt cggcgtgagc gcgatcgaca ccgacacggt ccgcatcctc
     4441 ggcaggccgt cctccgcgtg ccaggtctcg aagtccgagt gccagtagaa gccgctggcc
     4501 ccgaagccgg gcttgacgtt gacccgcgac tggtggacgt agacgtcgga gccgaggatc
     4561 tggcgggcgc gcccgacgat ccgcggatcc cgggcgagcc ggccgaagac ctcgctcatc
     4621 cggtgcacct cgaagacgga acgtatctcc tgggaacggg gctcgacgac cgcccgctcg
     4681 tcggcccgta cggccgggtc ggagacgagc cggtccagct ccgcccggag cacgtcgacc
     4741 tcgtccgggg tgacgatctc gtccaccgtg gcgaagccgt cgcgctcgaa ctcctccagt
     4801 tcacgggccc agaaggggcc cgccgttccg ggtccggacc acacgacggg gtccttgcgg
     4861 ccgatcagtt cttccttggg gccgcgggtg gggtagaggt cgacggggcg ggtgggtgcg
     4921 gagggcatgg ggagcgtgct tcctttcgcg tgggtgtgtc ggtcgcgtgc cggggctcgg
     4981 gcgtcggtcg cgtgccgggc tcaggcgtcg tcggacgcgg gctccggttc cgtcagcagc
     5041 gggtagacgc cgttctcgtc gtggtcctcg cgcccggtca cgggcgggtt gaagacgcac
     5101 aggcagcgga agtcggcctt gaccttgagg gtgtgccgct cgtgcccgtc gagcaggtac
     5161 atcgttccgg gcacgatcgt gtacgtgcgg cccgtctcgt ggtcggtgag ctcggcctcg
     5221 ccggccgtgc agacgacggc ctcgacgtgg ttcgcgtacc acatggcggt ctcggtgccg
     5281 gcgtacagca ccgtctcgtg gagggagaag ccgacccgct cacgggccag gacgatgcgc
     5341 ttgctctccc aggtgcccga ggcggccttc acgtgccgct cggtgttctc cagctcgtcg
     5401 aaggtacgga cgatcatggg gtacgactgc ctttcgggtc atcgggccgg ctgtgctgcc
     5461 ggccgctgga aggtggggtc gggcgcgggg tcaggcggtg tggcggacgg cgcgggccag
     5521 gatgccgagg ccctcgtcga gctcgtcgtc cgtcgcggtg agcggcggca gcagcttgac
     5581 cacctcgccg ctcgggcccg aggtctcgac cagcaggccg gtctcgaagg cgcggcggca
     5641 cacggcttcg gcgcgctccc cgtcggcgaa ctcgagaccc cacacgaggc cgcggccgcg
     5701 cacggacaga ccggtgcgcg cgtcgccccc gcacaggtcg aggagggcgc gctccacccg
     5761 gtccgcccgg cccagggtgc ggccgcgcag ggtgccgtcg cgccagtagg cgtcgagggc
     5821 ggcggtggcc gtgacgaagg cggggttgtt gccgcggaag gtcccgttgt gctcgccggg
     5881 ctgccagacg tcgagctccg gccggaacag gcagagggcc atggggagcc cgtagccgct
     5941 gatggacttg gacagcgtga cgatgtcggg cgtgatgccc gcctcctcga aggagaagaa
     6001 gtcgccggtg cggccgcagc ccatctggat gtcgtcgacg atgaggagca tctcgtgccg
     6061 gcggcagaga tcggccagcg cgcgcagcca ttcggcccgt gcgacgttga tgccgccctc
     6121 gccctggacg gtctcgacga tcacggcggc cgggtggtcg aggccggagc cggcgtcgtt
     6181 gaggagccgc tcgaaccaga ggaagtcggg gagctgcccg tcgaggtagt cgtcgaaggg
     6241 catccggatg gcgtgcggca gcgggacgcc cgcgccggcg cgcttggcgg cgttcccggt
     6301 gacggcgagc gagccgagcg acatgccgtg gaaggcgttg gtgaaggaga cgacggtctg
     6361 ccgcccggtg accttgcggg cgagtttcag cgcggcctcc acggcgttgg tgccggtcgg
     6421 gcccgggaac atcaccttgt agggcagggc ccggggctcc aggaccgtgg tgtggaaggc
     6481 ctcgaggaag tcgcgcttgg cggtggtcga catgtcgagg ccgtgggtga tgccgtcgtt
     6541 gccgaggtat tcgaggagag cccgcttgag cacggggttg ttgtggccgt agttcagcga
     6601 cccggcgccg gcgaagaagt cgaggtacgg acgcccctgc tcgtcgtaca gcctgctgcc
     6661 gaccgcgcgt tcgaagacga cgggccaggc gcggcagtag ctgcggacct ccgattcgac
     6721 ggtctcgaag acggagggcg tggcgatgtc ggtgagggtc acgaagctct tctccagggt
     6781 gttgttcggg tgggtggcgt acgcggtcga cggctcagga ggccggcggg ccgagggggc
     6841 cgatgtggta cagcacctcg gactcgtgtc ccgccgcggg gaacgaggag gccgggaaca
     6901 gcacctcctg cgtcacctcc gcgtcgtgcc ggcgtgcgaa cgaccggaac aaccggtcgg
     6961 aggcgaggtt ccccggggtg acggtggcct cgatccggct gatcccgtgc tccgccgcga
     7021 cctgcgcgga cagggcgtcg agcagagtcc cggctacccc gcttccgcgg tgcgaaccct
     7081 cgacggcgat ctgccagacg aagagggtct ccggctcgtc ggggcgcaga tatccggtga
     7141 cgaagccgat cgggcgtccg gaggcgtcac gggcgaccgt ggtcgtgccg gcgaagtcac
     7201 ggcaccacag cagatagctg tacggcgagt tgaggtcgag ttcgcccgag ccgcgtgcga
     7261 tccgccagag ttcggcgccg tccgcgagcg tcgggggagt ggtgcgaagg gtcgtggtcg
     7321 tcaggccagt ggtcaacggg gtcatgggcg ccgaagctac cgagtggatc tcggaacttc
     7381 ctggccctag ggggttgttg tggacaccca gccgtgatag atgggccgtc ggtcactgga
     7441 gggtgaaata tgtgccaggt cacatggaaa ggcgcacgaa ccaggacaga gtgaccgttt
     7501 gtaacggatg gataacgcct ctgaggtgtg tctgacgatc acgccaagag cgtgagaaga
     7561 tccgccattt cacgtttgaa ggcggccccg gcggtcaggc gtagaggctt ttccgtgaac
     7621 ggccccgagg cgccccgccc cggacgcgcg gaagccgggc cgggtacgac gaccccggcc
     7681 cggctcctgt caccgctgcg gcgctcggcc taccagatcg cctcgaccca ctccgggtgg
     7741 tcgatgaacg ggttccggtt gccctggtag gtggtgtgga tgatgtcgtt gcggcgctcc
     7801 tcgaaggcgc tcggcgggtc ctgctcgttc cactgcttca ggaccgagag gcgcccgtgg
     7861 aagcggacgc tgccgttggt ggtcgactcg ttgggctcca ggtcggccca ggcgtcgtcg
     7921 ccctcgtagc ggacggccat gtacaggatc atccgggcca cgtcgccctt gtcggcgtca
     7981 cgcggctcga acgagttgct gtccgtgtag ctgccggggg cgccgctgac cgcgctgccg
     8041 ccgttgtcga agtccttgtt gccccggatg ctgttgacct ggacgtccgc cgcccgcagg
     8101 tggtgcaggt ccgtgccggg gccggccgag gtcccgaagt ttccgtggga ctgggcccag
     8161 gtgtgctcgc ggttccagtc gccgacgtcg ccgccgttga ggctcttgct ccgcgaggtc
     8221 ccgctgtaga gcaggatgac gttgttggtg ttggcggggt cctggtcggt ggtcttcagc
     8281 gcgttccaga ccgcgtcgta cgagatcttg ctctggctgc tgatgatggt gtgcagcgag
     8341 gacttgaggc tcgtgccggt cttgcctatc gcgttcttgt agtacgtgct gtcgtacgcg
     8401 gtggtggtgg cgccggcggg ggtagcggcg accgtgggga gcgtgacgcc gacgagggtg
     8461 gcggcggctg ccgccgccca caccttccag ctgccgatcc gcacaacgga catggggggc
     8521 cctttcccgg ggacggtacg cgggggacgg cgggcgtcgg ctcgacgatg ccgctgtcta
     8581 cgcgtgttga cttcgtgtca ccgggagagt ggcacacggc aggtacctgt catgtaacga
     8641 ggagaaggct gttccatgac gagttcgcat atggccgaaa cgagtggtcg cctccccaag
     8701 gggccgcgcg cgcccgcctc ttggggtcgt ggtggcccgg agtgtcctca gccgccccgg
     8761 acgtccaccg gcatcccctt cggctccttg atgcgcttca tgatgatctg cgaattgacc
     8821 tcggtcaccc cggtgagggt cgtcagccgc tcgatccaca gccgctcgta cgccgcgagg
     8881 tcggcgaccg cgatccgcag caggcagccg gggctcccga acagccggta cgcctcgatg
     8941 acgtccggca cgtcccggag cgcctcctcg aaggcctcca ccgtctcccg gtcccgccgc
     9001 acctccaccg agacgagcac ctcgaagccc cgccccaccg cctccggatc gatgatcgcc
     9061 cggtacccct ggatcacccc gtcctgctcc agttgccgga cccggcgcag acagggcgac
     9121 gggctgagcc ccacccgctg ggccagctcc tggttgctca gccggccgtc gctctgaagc
     9181 tcgcgcaaga tgtggagatc gattcggtcc atggcgcaat tatctaccac gacgaaacga
     9241 tacggcgggc gatttcgcaa tcggcttgcg cgccttctgt cctatcgttg cgacgcaggg
     9301 tgatttcggt acggagtgag gacggacggc atgaagcgga cgaacgacgg gggagcgcgc
     9361 cggatcgtcg tcatcagcac gggcggcacg atagccagcc gctggcaggg caccggatac
     9421 gccgccgacg cctcgggcaa cgacgtgctg gccaccgccc cgcttcccga gggcgtcacc
     9481 gtcgaggtcg tcgacctctt caacgtcaac agctcccgca tgaccgcggc gcaccagctt
     9541 gccctgctca ggaccgtcca cgagaccctc gccgaccccg gcgtcgacgg catcgtgatc
     9601 acccacggca ccgacaccct cgaagagacc gccttcttcc tggacctcca ccacaccgac
     9661 gcccgcccgg tcgtgctcac cggcgcccag cgccccttcg gcaccgggga cggggacggc
     9721 cccggcaacc tctacgacgc gctccaggtc gcctcctccg tcagcgacct cggcgtcctc
     9781 gtcgtcttcg acgggcgcgt ccacgccgcc cgcggcacgg tcaagaccca gacgctcgcc
     9841 gccgacgcct tctccgaccc ctcggccgag cgcctgggcc gcgtcggctt ctcccgcgtc
     9901 gacatcgagc ggcagccgga gcgcccggcg ccgctgccgc tgcccgccgc cgcggagaag
     9961 gcggaccccg aggtccccgg tgccgtcccg ctgccccggg tcgccatcgt gacgcaccac
    10021 tccgacggcg atcccttcct cctgaacgcg gccgtcggcg ccggcgccga cggcatcgtc
    10081 ctcgtcgcca ccggcgcggg caacgccacc cccgagatcg ccgccgccgt ggccgacgcg
    10141 gtcgcccagg gcgtcctggt ggccgtgacc acccgtgtcc ccgccgggcc gctcgccgag
    10201 atctacaccg gcggcggtgc cgtcgacctc gtcgccgccg gggccctgct caccggcacg
    10261 ctccgcgccg cccaggcgcg gatcgcggtc ctcgccacac tcctcgccga aggcggcagc
    10321 ggcgcgggcg accccgccca cagcaccgcc ctcctgcgac gcctcctcga cgggcccgtc
    10381 cgcgcggaac cggccctcgc caccggcggc tcccgct
//
