LOCUS       NZ_LIWC01000003        10816 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Amycolatopsis sp. CB00013 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIWC01000003
VERSION     NZ_LIWC01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Amycolatopsis sp. CB00013
  ORGANISM  Amycolatopsis sp. CB00013
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 1488769)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488769)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905755.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:05:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,488
            CDSs (total)                      :: 8,430
            Genes (coding)                    :: 8,383
            CDSs (with protein)               :: 8,383
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 17 of 47
            Pseudo Genes (incomplete)         :: 38 of 47
            Pseudo Genes (internal stop)      :: 1 of 47
            Pseudo Genes (multiple problems)  :: 9 of 47
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:01:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1062975
            Orig. end                         :: 1073791
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIWC01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5816)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            118..1191
                     /gene="hemE"
                     /locus_tag="AMK34_RS24560"
                     /old_locus_tag="AMK34_24300"
     CDS             118..1191
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156993.1"
                     /locus_tag="AMK34_RS24560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24300"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_073846913.1"
                     /transl_table=11
                     /translation="MSPSVASPAQTVPAARRALPGAPFLAAARGERPAHTPVWFMRQAG
                     RSLPEYRALREGTSMFEACFDPEMLAEITLQPIRRHGVDAAILFSDIVVPLKAAGLDID
                     IVAGTGPVVAEPIRDAAGVRALPVLEPEQVERVAEGVRLLVGSLGETPLIGFAGAPFTL
                     ASYLIEGGPSRNHEHTKALMHSEPELWHELAGRLADMAITFLSAQLNAGADAIQLFDSW
                     AGALSERDYREFVLPHSAKVLSAVAGYGVPRIHFGVGTGELLVAMRDAGADVVGVDWRI
                     PLDEAVRRLGGGVVQGNLDPALLHASWPVIEAEVRRILAEGRAADGHIFNLGHGVLPGV
                     DPDVLTRVVGLVHELRP"
     gene            1188..2567
                     /gene="hemG"
                     /locus_tag="AMK34_RS24565"
                     /old_locus_tag="AMK34_24305"
     CDS             1188..2567
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332996.1"
                     /locus_tag="AMK34_RS24565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24305"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_073846914.1"
                     /transl_table=11
                     /translation="MKTVAVVGGGISGLTAAYRLRTLLGEDARIVVFESTGSLGGKLRT
                     IELAGERYDVGAEAFLARRPEALALVREIGLGESLVHPTKARAKIHAGGSVLGLPPGTV
                     MGVPASADAVAGVLSEKGRALVEAEASLSPLELPAGDVPLGPLLRERFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLASAVDRGARSLTEGAASLMPASPSTAPVFGTLLGGLG
                     TLIARLAELSRAEIRTGTTVTALERTADGWKVDDLDADSVLLAVPAPSARRLLDGVAGV
                     ASSAFAEVELASMAVVALALPPGTALPESSGVLIGAGERDAAGKPFAAKAFTFSSRKWA
                     QYGEGPVLVRGSVGRFGEPGALHADDDELVRVVREDLARLSGVTAEPIDTLVTRWGGGL
                     PQYGTGHLELVERIERAVAGVPGLAVAGATLHGVGLPACIATADAAARRIAGITPPHEG
                     S"
     gene            2593..3285
                     /gene="hemQ"
                     /locus_tag="AMK34_RS24570"
                     /old_locus_tag="AMK34_24310"
     CDS             2593..3285
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="AMK34_RS24570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24310"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_038515732.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEQGKLGEDRTKATAETTEYLDA
                     LEAKGVVVRGVYDLSGLRADADYMIWWHAEEIEQVQAAYSGFRHTPLGRASAPVWSQVA
                     LHRPAEFNKSHIPAFLAGEEARKFICVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTKVAP
                     AELIALLP"
     misc_feature    2635..2650
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            3297..4643
                     /locus_tag="AMK34_RS24575"
                     /old_locus_tag="AMK34_24315"
     CDS             3297..4643
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033530.1"
                     /locus_tag="AMK34_RS24575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24315"
                     /product="CoA transferase"
                     /protein_id="WP_073847177.1"
                     /transl_table=11
                     /translation="MDTRVWTTLTGDALAEDAVEITGPESVLPGTFRVEEAATTSIAAA
                     TLAAGELLRLRGIEPGKVSVDTRHAAASFHSEQYVRVDGEPVPFSAPLSGDYRANDGWV
                     RLHCNYPRHVSAVCWGLGVPATRAAFEKVVAAKSKYEVESAVVGAGGAAAVLRTRDEWL
                     AHKQGQASSALPVADIRPLGKSEPVRLFESDRPLGGVRILDLTHVIAGPVAGRVLAAHG
                     ATVMHIGSAQLPTVPPLAVDTGMGKLSAYVDLETEAGRSRLRKLIARADVVLQGFRPGS
                     LTAKGFSPEAIAELRPGIVIADLSAYGWEGPWARRRGFDSLVQMASGIADEGGKAAGLD
                     GPGPLPVQALDHATGWLTAAAIMTAVRRAATEGGSQHVRTSLAGTGEWLNSLGRKEAGP
                     AGFDGGEWLEDVDGQLGRVTRVKMAGTLPGSNPQWTEATHVPGSDRAVW"
     gene            complement(4660..5004)
                     /locus_tag="AMK34_RS24580"
                     /old_locus_tag="AMK34_24320"
     CDS             complement(4660..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033529.1"
                     /locus_tag="AMK34_RS24580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24320"
                     /product="hypothetical protein"
                     /protein_id="WP_073846915.1"
                     /transl_table=11
                     /translation="MTSPRERLAGQQAELLKALLAGGDAPAGFDADRLRIEANVLRNKQ
                     SRLAAYLRPDLAEALGDRFAALFREYATSRPKTDAIRARAYADAFGTWLVERGEVPKPR
                     GRFTRWLRRI"
     gene            complement(5001..5816)
                     /locus_tag="AMK34_RS24585"
                     /old_locus_tag="AMK34_24325"
     CDS             complement(5001..5816)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033528.1"
                     /locus_tag="AMK34_RS24585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24325"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073846916.1"
                     /sec_met_domain="DUF692 (E-value: 3.5e-81, bitscore: 268.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAADGPGRLGIGIGWRHELDLSIARLPGVDWVEVVAENLHARHLP
                     ETLVALRDKGMPVLPHAVSLSLGGAEPLDTARVEHLAEVTRELGAPLASDHVCFVRAGG
                     LDSGHLMPLPRTREALDVLVDNVKLTQSIVEVPFALENIAAVLEWPDAEFTESEFLREL
                     TDRTGCLLIVDVANLYANARNLGTDPERFLDEIPWERLAYTHMAGGVERDGVYHDTHAH
                     PVVPEVLDLLRELRRRTEPPGVLLERDDDYPPDTELAAELAALREAVSP"
     gene            complement(5806..6750)
                     /locus_tag="AMK34_RS24590"
                     /old_locus_tag="AMK34_24330"
     CDS             complement(5806..6750)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332999.1"
                     /locus_tag="AMK34_RS24590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24330"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073846917.1"
                     /transl_table=11
                     /translation="MEYTWGIPGPTFLTIYAILLVFPVVAGLVWTIAAKLGTRTPSAEV
                     SGPQPTVYEFAYLAGGSDRVVDTAIAALVDRGTLRVSSAKQLQLTGPEPADPIEKAVAK
                     SARPGHNATTRGIRDRLRLSGPMQALAKDLEGRGLVVVDQGPRIRRVVYFLYLAVLIFG
                     VVRLIAGLAGDRPVGFLIPMLIAAGGATLIARALKNKRPGPRPTAEGNRVLHKARSAHG
                     RERKRGIPNGPGYVGGAVLGGAAAAVAFGGLASYPDEELSAALIPPQVSSGFGGGSSGG
                     GSSCSSGGSSCSSGGSSCSSGGSSCGGGGGCGG"
     gene            complement(6864..7754)
                     /locus_tag="AMK34_RS24595"
                     /old_locus_tag="AMK34_24335"
     CDS             complement(6864..7754)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157012.1"
                     /locus_tag="AMK34_RS24595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24335"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073846918.1"
                     /transl_table=11
                     /translation="MNDPWGISGPAFAWLYGVCAVLPVALAVANEWWLRRDTGARTVPG
                     LYHLAALADGAERVTDTAVAGLIARHSLRPDGRGLLHPVEPAPADPFERAVVSLVHGNG
                     TGLRELRSKMARHEAVRALRADLEQRGLLVSEKHRRLGWQYAIALSVAVLALGIARLAN
                     AVPLHRPVGALVILIVGVIGAIGLAAWRQWPDGSGRATAAGTLALRYGDWDRGLARTAA
                     RQVALNGWQAFPDKVVAITLIRTGGTKARLFAGTASTCGGGGMYVAGSSCGGGGSSCGS
                     GGSSCGGGGGGGCGG"
     gene            complement(7830..8252)
                     /gene="msrB"
                     /locus_tag="AMK34_RS24600"
                     /old_locus_tag="AMK34_24340"
     CDS             complement(7830..8252)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333001.1"
                     /locus_tag="AMK34_RS24600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24340"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_034318341.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLSPDEYAVLRQAGTERPFTGEYTD
                     EKTTGVYQCRACGAELFRSDTKFESHCGWPSFYDPADTDAVLLREDTTMGMRRIEVLCK
                     SCHSHLGHVFEGEGYATPTDQRYCINSISLKLVPES"
     gene            complement(8272..8577)
                     /locus_tag="AMK34_RS24605"
                     /old_locus_tag="AMK34_24345"
     CDS             complement(8272..8577)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033523.1"
                     /locus_tag="AMK34_RS24605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24345"
                     /product="hypothetical protein"
                     /protein_id="WP_038515722.1"
                     /transl_table=11
                     /translation="MADSGPFGPRQNPRFQEDLVGLDPHDPEARAFAEHLDRIERCGPN
                     FTVEACLDGVSDFADSSNRLGGLRWWVSALVVVLIVLGVMVTAWDIIGRAIAWLAG"
     misc_feature    8640..8653
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.74"
                     /tool="antismash"
     gene            complement(8651..10300)
                     /locus_tag="AMK34_RS24610"
                     /old_locus_tag="AMK34_24350"
     CDS             complement(8651..10300)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157015.1"
                     /locus_tag="AMK34_RS24610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_24350"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_274534449.1"
                     /sec_met_domain="Pkinase (E-value: 5.2e-54, bitscore:
                     179.7, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIAGHYRLVEQIGSGAMGVVWRAIDVRLERSVAVKQILPQPGVSE
                     AERDNMRQRAMREAKNAARFQHPNAIVVFDIAEHGGDPCLVMEYLNGPSLSAIISDEGT
                     LPLGRVAKIGEQVASALVAAHRAGIVHRDVKPGNILIDESGTAKITDFGISRAAGDMTL
                     TQTGLIGGTPAYLAPELARGSDPVPSSDVFALGATLYQAIEGQTPYGNSSNQLALLYAA
                     ANGQINPPQQAGAATALLMSLLRTEPTERPSMAEARERLAALAANESGAGTAASPRLFG
                     AGGGRQPAVPETPGHGIPGGDRPPWQRTDKPAASAAAPPQPTKTHTPTAAFIPMRSPSA
                     PPNTPPRPVAAMSPPPSAAAPMRPSPAKPTDAKRKAVLFAGAGAAVVVIAVIVFLILNS
                     SNGGNTPGGNNAGANPQPGTSAPKSSAPSSSRPAPAGLGKTPSEKVTSYSSVGSFLLDF
                     FDTGKIGTSASWNKLTDAAKAVYGSEQAFAEYWAAHKVTGSEKARADSGGGNPDGSIDM
                     NITINGGPRPALRVVQVGGQYYIDADTRVLGN"
ORIGIN
        1 agatcgggta ctgacgtcat cgcggtcacc aaccgactgt aagacgcgat cgtccggttc
       61 ggggcgcaga cgcgccgaga gtaaggcctc cgagcgcaca tggcaccatg aaccgtgatg
      121 tctccttcag ttgcttctcc cgcgcagacc gtcccggcgg cccgacgcgc cctgcccggt
      181 gcgccgttcc tggccgccgc gcgcggcgaa cgcccggctc acacccccgt gtggttcatg
      241 cgccaggcgg gccggtcgct gcccgaatac cgggccctgc gcgagggcac ctcgatgttc
      301 gaggcgtgct tcgaccccga gatgctcgcc gagatcacgc tccagccgat ccgccgtcac
      361 ggcgtcgacg cggccatcct cttcagtgac atcgtcgtcc cgctcaaggc ggcgggcctc
      421 gacatcgaca tcgtcgcggg caccgggccg gtcgtcgccg agccgatccg cgacgccgcg
      481 ggcgtgcgcg cgctgccggt gctggagccg gagcaggtgg agcgggtcgc cgagggggtc
      541 cggctgctgg tcggctcgct gggcgagacg ccgctgatcg ggttcgccgg cgcgccgttc
      601 acgctggcct cctacctcat cgagggcggg ccgagccgca accacgagca caccaaggcg
      661 ctcatgcact ccgagcccga gctgtggcac gagctggcgg ggcgcctggc ggacatggcg
      721 atcaccttcc tctccgcgca gctcaacgcc ggggccgacg cgatccagct gttcgactcg
      781 tgggcgggcg cgctctcgga gcgggactac cgcgagttcg tcctgccgca ttcggcgaag
      841 gtgctgtccg cggtcgcggg ctacggcgtg ccccggatcc acttcggtgt cggaaccggc
      901 gagctgctgg tggccatgcg tgacgcgggc gccgacgtgg tcggcgtcga ctggcgcatc
      961 ccgctcgacg aagccgtgcg gcggctcggc ggcggcgtgg tccagggcaa cctcgacccg
     1021 gcgctgctgc acgcgtcctg gccggtcatc gaggccgaag tgcggcgcat cctcgccgag
     1081 ggccgcgcgg ccgacggcca catcttcaac ctcggccacg gcgtgctgcc cggcgtcgac
     1141 ccggacgtac tgacacgcgt ggtcgggctg gtgcacgagc tccggccgtg aagacggtcg
     1201 ccgtcgtcgg cggcggtatc tcgggactga ccgcggcgta ccggctgcgg accctgctcg
     1261 gcgaggacgc caggatcgtc gtcttcgagt cgaccgggtc gctcggcggg aaactccgca
     1321 cgatcgaact cgccggtgaa cgctacgacg tcggcgccga ggccttcctc gcccgccgtc
     1381 ccgaagcgct cgccctggtc cgcgagatcg ggctgggcga aagcctcgtc catcccacca
     1441 aggcccgcgc caagatccac gcgggcggct ccgtgctcgg cctgccgccg ggcacggtca
     1501 tgggtgttcc cgcttccgcc gacgcggtcg ccggggtgct gtcggagaag ggccgcgccc
     1561 tcgtcgaggc cgaggcgtcg ctgtccccgc tggaactgcc cgccggcgac gtgccgctcg
     1621 gcccgctgct gcgcgagcgg ttcggtgacg aactggtcga ccggctcgtc gatccgctgc
     1681 tcggcggggt gtacgccggg ggagcggacg ggctcggcct gcgcgcgacg atgcctgggc
     1741 tcgcctccgc ggtggaccgc ggcgcgcgtt cgctcaccga aggcgcggcg tcgctcatgc
     1801 ccgcctcgcc gagcacggcg ccggtcttcg gcaccctgct gggtggcctc gggacgctga
     1861 tcgcgcggct cgcggagctg tcccgcgccg agatccgcac cgggaccacg gtgacggcgc
     1921 tcgaacgcac cgccgacggc tggaaggtcg acgacctcga cgccgactcc gttctcctgg
     1981 cggttccggc gccttcggca cggcggctgc tcgacggcgt cgcgggtgtg gcgtcttcgg
     2041 cgttcgccga ggtcgaactc gcgtcgatgg ccgtcgtcgc gctcgcgctc ccgccgggta
     2101 ccgcgctgcc ggagtcgtcc ggcgtgctga tcggtgccgg ggagcgggac gccgccggga
     2161 aaccgttcgc cgccaaggcg ttcaccttct cgtcccgcaa atgggcgcag tacggcgaag
     2221 gcccggtgct ggtgcgtggc tcggtcggcc gcttcggcga accgggcgcg ctgcacgccg
     2281 acgacgacga actcgtgcgc gtggtccgcg aagacctcgc ccggctgagc ggagtcaccg
     2341 ccgagccgat cgacaccctg gtgacccgat ggggtggcgg gctgccgcag tacggcaccg
     2401 ggcacctcga actcgtcgag cggatcgaac gcgccgtcgc cggtgtcccg gggctcgccg
     2461 tggcgggggc gacactgcac ggcgtcgggc tcccggcctg catcgccacc gccgacgccg
     2521 ccgcccgccg catcgccggg atcacaccgc cccacgaggg ctcctgagcg cgggcagtgc
     2581 caagatggcg gtatggcgcg gctgaactac aacgagctca atgacaccat ccgctacacc
     2641 gcctggtcgg tcttccgggc cgagcagggc aagctcggcg aagaccgcac gaaggcgacc
     2701 gcggagacca ccgagtacct cgacgcgctg gaggccaagg gcgtcgtcgt ccgcggtgtc
     2761 tacgacctct ccgggctgcg cgccgacgcc gactacatga tctggtggca cgccgaggag
     2821 atcgagcagg tccaggcggc gtacagcggt ttccggcaca ccccgctcgg ccgcgcgtcg
     2881 gcgccggtgt ggagccaggt cgcgctgcac cgcccggcgg agttcaacaa gagccacatc
     2941 ccggcgttcc tcgccggtga agaggcccgc aagttcatct gcgtctaccc gttcgtgcgc
     3001 tcctacgagt ggtacctgct gccggacgac gagcgccgca agatgctcgc cgaccacggc
     3061 aaggaggccc gcgactaccc ggacgtccgc gccaacacgg tcgcgtcgtt cgcgctgggc
     3121 gactacgagt ggatcctggc cttcgaggcc gacgaactgc accgcatcgt cgatctgatg
     3181 cgtcatctgc gcggcaccga ggcccgcctg cacgtgcgcg aggagatccc gttctacacc
     3241 gggacgaagg tggcgcccgc cgagctgatc gcgctcctgc cctagtgaag gcctccgtgg
     3301 acacccgcgt ctggaccacg ctcaccggcg acgccctcgc cgaagacgcc gtcgagatca
     3361 ccggccccga gtcggtgctg cccgggacct tccgggtcga ggaggcggcc acgacgagta
     3421 tcgccgcggc gacgctcgcg gccggggaac tgttgcggct gcggggaatc gagccgggca
     3481 aggtctcggt cgacacccgg cacgccgccg cgtcgttcca cagcgagcag tacgtccgcg
     3541 tcgacgggga gccggtgccg ttcagcgcgc cgctgtccgg cgactaccgc gcgaacgacg
     3601 gctgggtgcg gctgcactgc aactacccgc gccacgtgtc cgccgtgtgc tgggggctcg
     3661 gcgttcccgc gacccgcgcg gcgttcgaga aggtcgtggc ggccaagtcg aagtacgagg
     3721 tcgagtcggc cgtggtcggc gcgggtggtg ccgcggccgt gctgcgcacg cgtgacgagt
     3781 ggctggcgca caagcaggga caggcgtcga gcgcgttgcc ggtcgccgac atccggccgc
     3841 tcgggaagtc ggaacccgtg cggctcttcg aatccgaccg gccgctgggc ggggtgcgga
     3901 tcctggacct gacccacgtc atcgccgggc cggtcgcggg ccgcgtgctc gccgcgcacg
     3961 gggcgaccgt catgcacatc ggttcggcgc agctgccgac cgtgccgccg ctggccgtcg
     4021 acaccgggat ggggaagctc tcggcgtacg tcgacctgga gaccgaagcc gggcgctcgc
     4081 ggctgcgcaa actgatcgcc cgcgccgacg tcgtgctgca gggtttccgg ccgggttcgt
     4141 tgaccgccaa gggtttctcc ccggaagcga tcgccgagct gcggccgggc atcgtgatcg
     4201 cggacctgtc cgcctacggc tgggaagggc cgtgggcacg caggcgcggg ttcgacagcc
     4261 tggttcagat ggcgtccggg atcgcggacg agggcggcaa ggcggccggg ctcgacggcc
     4321 cgggaccgct tccggtgcag gctctcgacc acgcgaccgg ctggctcacc gcggcggcga
     4381 tcatgaccgc cgtccgacgg gcggccaccg aggggggcag ccagcacgtc cgcacctcgc
     4441 tcgccgggac gggggagtgg ctgaactcgt tggggcgtaa ggaagccgga cccgccgggt
     4501 tcgacggcgg cgagtggctc gaagacgtcg acggccagct cggccgggtc acccgggtga
     4561 agatggcggg caccctgccg ggctcgaatc cacaatggac ggaagcgacg cacgtaccgg
     4621 ggagcgaccg cgcggtttgg tgaaaccgca ttcagaggac taaatgcggc ggagccagcg
     4681 tgtgaaccgg cctcggggct tcgggacctc gccgcgctcg acgagccacg tgccgaaagc
     4741 gtccgcatag gcccgcgcgc ggatcgcgtc ggtcttcgga cggctcgtcg cgtactcgcg
     4801 gaagagcgcg gcgaaccggt caccgagggc ttcggcgagg tccgggcgga ggtaggcggc
     4861 cagcctgctt tgcttgttcc gcaagacgtt cgcctcgatg cggagccggt ccgcgtcgaa
     4921 gcccgccggg gcgtcgcctc cggcgagaag cgccttgagc agctccgcct gctgcccggc
     4981 gagccgctcc cggggcgacg tcacggcgag accgcttccc gcagcgcggc cagctccgcc
     5041 gccagttcgg tgtcgggcgg gtagtcgtcg tcgcgttcca gcaggacgcc gggcggttcg
     5101 gtgcgccgcc gcagttcgcg cagcaggtcc aggacctccg gcaccaccgg atgggcgtgc
     5161 gtgtcgtgat agacgccgtc ccgttcgaca ccgcccgcca tatgggtgta cgccaggcgc
     5221 tcccacggga tctcgtcgag gaaccgctcg ggatcggtgc cgaggttgcg ggcgttggcg
     5281 tacagattgg ccacgtcgac gatcagcagg cagccggtcc ggtcggtgag ctcccgcagg
     5341 aactccgact cggtgaactc cgcgtccggc cattccagta ccgccgcgat gttctccagc
     5401 gcgaacggga cctccacgat ggactgcgtc agtttgacgt tgtccaccag gacgtcgagc
     5461 gcctcccggg tgcgggggag cggcatcaga tggcccgagt cgaggccgcc cgcgcgcacg
     5521 aagcacacgt ggtcgctggc cagcggtgcg ccgagctcgc gggtgacctc cgcgagatgc
     5581 tcgacgcgcg cggtgtccag cggttcggct ccgcccagcg acagcgagac cgcgtgcgga
     5641 aggaccggca ttcccttgtc gcgcaaggcg accagggtct ccggcagatg ccgggcgtgc
     5701 aggttctcgg cgacgacctc gacccagtcg acgcctggca gccgcgcgat cgaaaggtcg
     5761 agctcgtgcc gccagccgat gccgatcccg agcctgccgg gcccgtcagc cgccacagcc
     5821 gccgcctcca ccgcaggagc tgccgccgct gctgcacgag ctcccgccgc tgctgcagga
     5881 gctcccgccg ctgctgcacg aagaaccgcc gccggacgaa ccgccgccga aaccgctgga
     5941 gacctgcggc gggatcaggg ccgcgctcag ctcctcgtcg gggtaagagg cgaggccgcc
     6001 gaacgcgacc gccgccgcgg caccacccag cacggcgccg ccgacgtagc ccggtccgtt
     6061 cgggatgccg cgtttgcgct cccgcccgtg ggcggacctg gccttgtgga ggacacggtt
     6121 gccttcggcg gtcggccgcg ggcccggccg cttgttcttg agcgcacggg cgatcagggt
     6181 cgcgcccccg gccgcgatca gcatcgggat caggaatccg accgggcggt cgccggcgag
     6241 cccggcgatc aggcgcacca caccgaagat cagtacggcg aggtacagga agtacacgac
     6301 ccggcggatc cgtggcccct gatcgacgac gaccagcccg cgtccctcca gatccttggc
     6361 gagcgcctgc atcggacccg acagccgcag ccggtcacgg atgccgcggg tcgtggcgtt
     6421 gtgccccggc cgcgcgctct tggcgacggc cttctcgatc gggtcggcag gctcgggacc
     6481 ggtgagctgg agctgcttcg cgctgctgac ccgcaaggtc ccgcggtcga ctagcgccgc
     6541 gatggccgtg tcgaccaccc ggtccgagcc gccggcgaga taggcgaact cgtataccgt
     6601 cggctgcggc ccgctcacct cggcgctcgg ggttctggtg cccagcttgg ccgcgatcgt
     6661 ccacacgaga ccggcgacca ccgggaacac cagcaggatg gcgtagatcg tcaggaacgt
     6721 gggtccgggg atgccccagg tgtattccat cgcacgggcc tttcacgagc aggtgcactt
     6781 accgtagtcg gacgccgcgc ggtccgatcg tggttccgtg attcgagcat ccggcgcgga
     6841 gtccggcgac ggcagttggg ccgtcagccg ccgcagccgc ctccaccgcc gccgccgcag
     6901 gaagaacctc cactgccgca cgaggagcct ccgccgccgc acgaggaacc ggcgacgtac
     6961 atgccgcctc cgccgcacgt gctcgccgta ccggcgaaga gcctggcctt cgtgccgccg
     7021 gtcctgatca gcgtgatcgc gacgaccttg tccgggaagg cctgccagcc gttgagcgcc
     7081 acctgcctgg ccgcggtgcg ggccaggccg cgatcccagt cgccgtaccg gagcgccagc
     7141 gtgcccgcgg cggtcgcccg gccggagccg tccggccatt gccgccacgc ggccaggccg
     7201 atcgcgccga tcacgccgac gatcaggatc acgagggcac cgaccggccg gtgcagcggt
     7261 acggcgttcg ccagccgcgc gatccccaag gccaagacgg ccacggacaa ggcgatcgcg
     7321 tactgccagc ccagccggcg gtgtttctcg gagaccagca ggccacgctg ttcgagatcg
     7381 gcgcgcaatg ccctgacggc ctcgtgccga gccatcttcg agcgcagctc acgcaggccg
     7441 gtcccgttgc cgtggacgag cgagacgacc gcgcgctcga aggggtcggc gggggccggc
     7501 tcgaccgggt gcaggaggcc gcgtccgtcc ggccgcaggg agtgcctcgc gatcaggccc
     7561 gcgacggcgg tgtcggtgac ccgctccgcg ccgtccgcca acgcggccag gtgatagaga
     7621 ccgggcaccg tcctggcacc ggtgtcacgg cgcagccacc actcgttcgc cacggcgagc
     7681 gcgaccggca gtacggcaca gactccgtac agccacgcga aagccggtcc ggagatcccc
     7741 caagggtcgt tcataggaca ccgccctccc cgacgctgtt cttgttcctc gtgaacgcgt
     7801 cgagaagggc ggtggttcgc ttcgttcggt tacgactcgg gaacgagctt cagcgagatc
     7861 gagttgatgc agtaccgctg atccgtcggc gtggcgtaac cctcgccttc gaagacgtgc
     7921 ccgaggtggc tgtggcacga cttgcacagc acctcgatcc ggcgcatccc catggtggtg
     7981 tcctcgcgga ggaggacggc gtcggtgtcg gccgggtcgt agaacgacgg ccagccgcag
     8041 tggctctcga atttcgtgtc gctgcggaac agctcggcgc cacaggctcg gcactggtag
     8101 acaccggtgg tcttctcatc ggtgtactca ccggtgaagg ggcgctcggt gcccgcctgg
     8161 cgaaggacgg cgtactcgtc ggggctgagc tgctcccgcc attcctgctc ggacttgacc
     8221 acgcgcggcg tggctccgac aacgggtttc atgcctttca ccggttccac gttaccccgc
     8281 gagccacgcg atggcccggc cgatgatgtc ccatgcggtg accatcacgc cgagcacgat
     8341 gaggaccacg accagtgccg aaacccacca gcgcaggccg ccgagccggt tgctggagtc
     8401 ggcgaaatcg gagacaccgt cgaggcaggc ctccacggtg aagttcggcc cgcagcgctc
     8461 gatgcggtcc aggtgctcgg cgaaggcccg ggcctcgggg tcgtgcgggt cgaggcctac
     8521 caggtcttcc tggaagcgag gattctggcg aggtccgaaa ggtccgctat cggccatacc
     8581 ggcaacagta agccacgatc ggcgattggc ctaccaccag cgaggcgggt ctcacccccg
     8641 ccacgctgcg tcaattgccg agtactcgcg tgtcggcgtc gatgtagtac tgcccgccga
     8701 cctggaccac gcgcagcgcc ggccgcgggc cgccgttgat cgtgatgttc atgtcgatcg
     8761 acccgtcggg gtttccgccg ccgctgtcgg ctcgtgcctt ttcggagccg gtgaccttgt
     8821 gcgcggccca gtattcggcg aaagcctgct cggagccgta cacggccttc gccgcgtcag
     8881 tgagcttgtt ccagctcgcg gacgtgccga tcttgccggt gtcgaagaag tccaggagga
     8941 agctgcccac gctggagtag ctggtgacct tttcgctggg ggtcttcccc aggcccgcag
     9001 gcgcgggacg cgacgaggac ggggcgctcg acttgggcgc cgaggtgccc ggctgcgggt
     9061 tcgccccggc gttgttccca cccggggtgt tgcccccgtt gctcgagttc aggatcagga
     9121 acacgatcac ggcgatcacc acgaccgcgg caccggcgcc cgcgaacagg acggccttgc
     9181 gcttcgcgtc ggtcggcttg gcgggtgacg gccgcatcgg ggcggccgcg gacggcggcg
     9241 ggctcatcgc ggcgaccgga cgcggcgggg tgttcggcgg ggcggacggc gagcgcatcg
     9301 ggatgaacgc ggcggtcggc gtgtgggtct tggtgggttg cggaggagcc gcggcggagg
     9361 cggccggctt gtcggtccgc tgccacggcg ggcggtctcc gccggggatg ccgtgtccgg
     9421 gggtctcggg cacggcgggc tgtctaccgc cgcccgctcc gaaaagccgc ggcgaggcgg
     9481 ccgtgcccgc accggattcg ttcgccgcca gcgcggcgag gcgctccctg gcctcggcca
     9541 tgctcgggcg ctcggtcggc tcggtgcgca gcaggctcat cagcagcgcg gtcgcggcac
     9601 ccgcctgctg cggcgggttg atctggccgt tcgcggccgc gtacagcagc gcgagctggt
     9661 tggagctgtt cccgtacggc gtctggccct cgatcgcctg gtagagcgtc gcgccgagcg
     9721 cgaagacgtc ggagctgggc accgggtccg aaccgcgcgc aagctcgggt gcgaggtacg
     9781 ccggggtgcc gccgatcagg ccggtctggg tgagcgtcat gtcacccgcc gcgcgggaga
     9841 tgccgaagtc ggtgatcttc gcggtgccgg actcgtcgat caggatgttg cccggtttga
     9901 cgtcgcggtg cacgatcccg gcgcggtgcg cggcgaccag tgccgacgcg acctgctcgc
     9961 cgatcttcgc gacgcgcccc agcggcagcg tgccttcgtc ggagatgatc gccgagaggc
    10021 tcgggccgtt caggtactcc atcaccaggc aggggtcgcc gccgtgctcg gcgatgtcga
    10081 acaccacgat cgcgttcgga tgctggaacc gggccgcgtt cttcgcctcg cgcatggcgc
    10141 gctggcgcat gttgtcgcgt tcggcctcgg agacacctgg ctgcgggagg atctgcttga
    10201 ccgccaccga gcgctcgagg cggacatcga tggcacgcca gaccaccccc atggcaccgc
    10261 taccaatttg ctcgaccagg cgatagtgcc cagcgatcag ctgaccggtg tcgatggcga
    10321 ctgctcctga cacattctcc gggtgatggt ggcgttacgc ggcctgacag gtcccgcgca
    10381 cccgatcgag tgtagcgggc ggcggtttcg gctccccgtc agctcgccgg tgcggaggcg
    10441 ggcaattctt cctcgccgct cggttcgggt agcggagggg ccttgcgccg gaagaccttg
    10501 accagcccga tggcccccgc ggcggcgaag acggcgtaac agatcagcag ggcgggaggt
    10561 gtttccccgg tcagtgcgtc gccgaggacg acgacggcga ccgtacccgg caggctgccg
    10621 agcgcggtcc cgatcagata cggggcgaaa cggaccgatg acactccgca caggtaactg
    10681 aacggagcga acggcaccat cgggatcagc cgtaaggagg tgatcgccag gactccgccg
    10741 tccgaaagcc ggtcgttgac cgcgcggacc ttcgcccggt gcaggtgccg ggtgaccaga
    10801 tcgcggccga gtgccc
//
