LOCUS       NZ_LIWC01000002        10390 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Amycolatopsis sp. CB00013 scaffold2, whole genome shotgun sequence.
ACCESSION   NZ_LIWC01000002
VERSION     NZ_LIWC01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Amycolatopsis sp. CB00013
  ORGANISM  Amycolatopsis sp. CB00013
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 1966077)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966077)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905755.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:05:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,488
            CDSs (total)                      :: 8,430
            Genes (coding)                    :: 8,383
            CDSs (with protein)               :: 8,383
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 17 of 47
            Pseudo Genes (incomplete)         :: 38 of 47
            Pseudo Genes (internal stop)      :: 1 of 47
            Pseudo Genes (multiple problems)  :: 9 of 47
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:01:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 600293
            Orig. end                         :: 610683
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIWC01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5390)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    71..89
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.56"
                     /tool="antismash"
     gene            complement(580..3369)
                     /locus_tag="AMK34_RS12840"
                     /old_locus_tag="AMK34_12730"
     CDS             complement(580..3369)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631926.1"
                     /locus_tag="AMK34_RS12840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_12730"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_073845397.1"
                     /transl_table=11
                     /translation="MAESGSPARFQLLGPVRLLDGDRPVPVGGPGVRGLLALLALKVNK
                     VVALDEIIDALWGHDPPATARTIVHGNVSHLRRVLRGIQGDHPDGAQIRTTPPGYQLTV
                     EPERIDVHRARSLLERASTEEPEKASELLAEALSLWQGPALAGVPSSVRAPELEDLRLA
                     VHGARVDADLELGRHAELIVELSPLVRAAPLAERTAGQLMRALYHAGRRGDALELYRTV
                     SRATLRTLGVEPGADLRWLHERVLNDDLPVKVVAEPKPEAGPVQQLPPAVPNLAGREAD
                     LAWLDELAERAEAGETAVGVVTGTAGIGKSSLVVWWAHRAARRFPDGILFAALRGFDPH
                     HPPLEPAELLTQFLLGLGVPAEGVPEQVHERVALYRSMIAGRRMLVILDNARSADQVRP
                     LLPPGSRSMTLVTSRSRLDGLAVSNAAKLRVLGTLAPGDAVRLIEELAGPAGYDLNHAL
                     ARLCGYLPLALRIAGARLAASAQWSAQDLVDELGNERTRLAALDVEGPDDGVRAAFDVS
                     FRGLPAEVANTFLRLGVVQCVSAGSHLTAAIGGITVAEARRHLRVLAAHNLLAETGRDS
                     FVPHDLVRLFLRELAENELDEADREDVLSRSVRFYQAVADRARRKMLRIVDPLDFTAVL
                     TDAQTPPIGTFDEAQDWFTAEWENLLEVLEAARAAGRHDDVWRLARVAHTYRAVYPLLD
                     EWTRLVGIGLEAAERSGDVLGQCWMLISRCAIALTFELPQGCLADAERALELASAIGDD
                     RLMVSANIHLGSALTLLGRYDEAIGTLRRAVEETERTGDLELRGQALNNCAEAEKRAGR
                     FIEAIGHQIASLEIDRTLGDDSYVVVSLNNLAELSMRIGELAAAERYVWEAVDLTISRR
                     FVLQEGVLRLTLGRVLRAGSDVDGAREQFALALKILADANPKLAGLVRAELTDLGESP"
     misc_feature    3664..3675
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(3707..3718)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(4077..4976)
                     /gene="thpD"
                     /locus_tag="AMK34_RS12845"
                     /old_locus_tag="AMK34_12735"
     CDS             complement(4077..4976)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="AMK34_RS12845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_12735"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073845399.1"
                     /sec_met_domain="PhyH (E-value: 1.8e-32, bitscore: 110.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVDDSYPTRITGKPEHLPRTHPTVWGTEADGPLDAATLA
                     NHETRGYTVVDQLLSPGEIQTYWQELVRMSSGDHRDDERVITEAKTGEVRSIFDVHETS
                     ELIAELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPL
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGETPEDNYKSSLKEQRVGVPSEEDI
                     TSLAAEYGIDQFTGQAGSALWFDSNVMHGSSNNITPYPRSNIFVVFNSVENALQKPYAA
                     IEPRPAFIAGRDSTPLTR"
     gene            complement(5001..5390)
                     /locus_tag="AMK34_RS12850"
                     /old_locus_tag="AMK34_12740"
     CDS             complement(5001..5390)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="AMK34_RS12850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_12740"
                     /product="ectoine synthase"
                     /protein_id="WP_007033454.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.7e-54, bitscore:
                     179.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEVTDTDADIKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGELENTATGEVFQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPITGREVHDENGVYPLVTEP"
     gene            complement(5435..6685)
                     /gene="ectB"
                     /locus_tag="AMK34_RS12855"
                     /old_locus_tag="AMK34_12745"
     CDS             complement(5435..6685)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 8.6e-73.
                     Score: 237.2. Matches aSDomain:
                     nrpspksdomains_AMK34_RS12855_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033455.1"
                     /locus_tag="AMK34_RS12855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_12745"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073845401.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.8e-75, bitscore:
                     249.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSYLYDEDGKAYLDFFAGA
                     GALNYGHNNPALKRALIDYIARDGVTHALDMFTVAKRDFLQTFQEKILRPRDLDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDNYFDGSIPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARVEWLKALD
                     DLCKKHGILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPD
                     LDVWEPGEHNGTFRGISPAFVTATEAMRVYWSDDELEKSTKAKGERVVAAFQGIVEAYP
                     DANLFAKGRGLARGIEFANGDLAGKVCAAAFERGLLMETSGPDGEVMKVLPPLTLTDDE
                     LTKGLAIIDEAVAAVLN"
     aSDomain        complement(5612..6622)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK34_RS12855_Aminotran_3.1"
                     /evalue="8.60E-73"
                     /label="AMK34_RS12855_Aminotran_3.1"
                     /locus_tag="AMK34_RS12855"
                     /protein_end="358"
                     /protein_start="21"
                     /score="237.2"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGKAYLDFFAGAGALNYGHNNPALKRALIDYIA
                     RDGVTHALDMFTVAKRDFLQTFQEKILRPRDLDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDNYFDGSIPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARVEWLKALDDLCKKHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPDLDVWEPGEHNGTFRGISPAFV
                     TATEAMRVYWSDDELEKSTKAKGERVVAAFQGIVEAYPDANLFAKGRGLARGIEFA"
     misc_feature    6716..6731
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(6724..7215)
                     /gene="ectA"
                     /locus_tag="AMK34_RS12860"
                     /old_locus_tag="AMK34_12750"
     CDS             complement(6724..7215)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033456.1"
                     /locus_tag="AMK34_RS12860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK34_12750"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_038507771.1"
                     /transl_table=11
                     /translation="MSGKHLIETPTKADGAALWRIARDSKKLDLNTPYAYLLWCHDFAD
                     TSVVAKVDGVPVGFVIGYRKQDTGFVWQVAVDASQRGKGLAGALLDELFSRLTAQGVRY
                     LETTITPDNEASIRLFASFAKRWDTTVERQDLFSATDFPAEEGTHEQEDLYRIGPLSSQ
                     "
     misc_feature    complement(8056..8074)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.86"
                     /tool="antismash"
     misc_feature    complement(10152..10166)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    10153..10167
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
ORIGIN
        1 cacgtcccgg ccgtcgccgg ggacaagaac accgtcgagt tcgtcgtcag cgcctcgggc
       61 ggcgcgacca tccggttcgg ctcgctcaac gaccagcgga ccgagacgac cccggcgagc
      121 accgacgaat ggcgcggcac ggcgtccttc aactcgggcg actacctgct caccgtcacc
      181 gcggacaccc ggaactcgtc ggtcagcaac cagatcgcct gcaccatcct ggtcaacggc
      241 cagaaggtgg cggagaacaa ggggcagacc atcgccctgt gcaccgccgg caccggctga
      301 ccccacacgt ttcgtcctct gaatgcggtt cttgcgtgtg caagtaccgc attcagagga
      361 cgaaacgcgg tggtgccgcc tggcggccgg ccggtatcgc ccccacctcc accggccgga
      421 ccgccacgag ccgctcctgg cggctcttca gacgggcccg cgaagggccc gcccgagcgc
      481 cgattcctac cgcatgcccc tcccctaggg ctcgatcgac gctgaatcgg accctacgcg
      541 cgtgcgcggc gaggccacta agaatccgct aaggatcact cacggactct cgccgaggtc
      601 cgtgagttcc gcacggacca atcccgcgag cttcggattg gcgtccgcga ggatcttcag
      661 cgcgagcgcg aactgctcgc gggcaccgtc cacatccgac cctgcccgca acaccctgcc
      721 caaggtcagc cgcagtacgc cctcctgcag gacgaaccgc ctgctgatcg tcaggtccac
      781 ggcctcccag acgtaccgtt cggccgcggc gagttcgccg atccgcatgc tcagttcggc
      841 gagattgttc agcgagacga cgacgtagct gtcgtcgccg agggtccggt cgatctccag
      901 cgaggcgatc tggtgcccga tcgcctcgat gaaccgcccg gcccgctttt cggcctcggc
      961 gcagttgttg agcgcctgcc cgcgcagttc gaggtctccg gtccgctcgg tctcctcgac
     1021 cgcccgccgc aacgtcccga tggcctcgtc gtaccggccg agcagcgtca gcgcggagcc
     1081 gaggtggatg ttcgccgaga ccatcaggcg gtcgtcgccg atcgcgctcg cgagttccag
     1141 cgcgcgttcg gcgtcggcga ggcagccctg cggcagttcg aaggtgagcg cgatggcgca
     1201 ccgggagatc agcatccagc actggccgag gacgtcgccg gaccgttcgg cggcctccag
     1261 cccgatcccg accagccggg tccattcgtc cagcaacggg tacaccgcgc gataggtgtg
     1321 cgcgaccctg gccagccgcc agacgtcgtc gtgccgtccg gcggcgcggg cggcttcgag
     1381 gacttcgagc aggttctccc attccgcggt gaaccagtcc tgcgcctcgt cgaaggtgcc
     1441 gatcggcggc gtctgcgcgt cggtcagcac agcggtgaaa tcgagggggt cgacgatccg
     1501 cagcatcttg cggcgggccc ggtcggcgac ggcctgatag aaccgcactg accgcgacag
     1561 gacgtcctcc cggtcggcct cgtcgagttc gttctccgcc agttcgcgca ggaacagccg
     1621 gacgagatcg tgcggcacga aggaatcgcg gccggtttcg gcgagcaggt tgtgcgcggc
     1681 gagcacccgc agatgccgcc gtgcctcggc gacggtgatc ccgccgatcg cggccgtcag
     1741 atgcgaaccg gccgaaacgc attggacgac gccgagccgc aggaacgtgt tcgcgacctc
     1801 ggcgggcaga ccgcggaacg agacgtcgaa cgccgcgcgg accccgtcgt ccggcccttc
     1861 gacgtcgagc gcggccagcc tggtccgctc gttgccgagt tcgtcgacca gatcctgcgc
     1921 ggaccactgc gcgctggcgg cgagccgggc tcccgcgatc cgcagcgcca gcgggagata
     1981 gccgcacagc cgggcgagcg cgtggttgag gtcgtagccg gcgggcccgg cgagctcctc
     2041 gatcaaccgg acggcgtcgc cgggcgcgag cgtgccgagc acccgcagtt tggcggcgtt
     2101 ggagacggcg agcccgtcga ggcgggagcg gctggtcacc agcgtcatcg accgcgatcc
     2161 tggtggcagc aacggccgga cctggtcggc tgagcgggca ttgtcgagga tgaccagcat
     2221 ccggcggccg gcgatcatcg accggtagag ggcgacgcgt tcgtggacct gttccggaac
     2281 gccttcggcc gggacgccga gcccgagcag gaactgggtg agcagctccg cgggctccaa
     2341 cggcggatga tgcgggtcga acccgcgcag cgcggcgaaa aggatgccgt cggggaagcg
     2401 gcgggccgcg cgatgcgccc accacaccac caggctgctc ttgccgatcc cggcggtgcc
     2461 ggtgaccaca ccgaccgcgg tctcgcccgc ttcggcccgc tcggcgagtt cgtcgagcca
     2521 ggccagatcc gcctcgcgtc cggcgagatt cggcaccgcg ggcggcaact gctggacggg
     2581 ccccgcctcg ggcttcggct ccgcgacgac cttgaccggc aggtcgtcgt tgaggacgcg
     2641 ttcgtgcagc cagcgcaggt ccgcgccggg ttcgacaccg agggtgcgca gggtcgcccg
     2701 ggagaccgtg cgatagagct cgagcgcgtc gccgcggcgt ccggcgtgat acagcgcgcg
     2761 catcagctga ccggcggtgc gctcggcgag cggcgcggcc cggaccagcg ggctgagctc
     2821 gacgatcagt tccgcgtgcc tgccgagttc gagatccgcg tcgaccctgg cgccgtggac
     2881 ggcgagccgc agatcctcca gctcgggagc gcgaaccgaa ctcggcacgc ctgccagcgc
     2941 gggcccctgc cagagtgaga gggcctccgc gagcagttcg gacgccttct ccggctcttc
     3001 cgtcgaagcc cgctccagca gggaccgggc gcggtggacg tcgatccgct ccggctcgac
     3061 cgtgagctgg tagccgggcg gcgtggtgcg gatctgggca ccgtccgggt gatcgccctg
     3121 gatgccccgg agcacgcggc gcagatgcga aacgttgccg tggacgatcg tgcgggcggt
     3181 ggcgggcgga tcgtggcccc agagcgcgtc gatgatctcg tcgagcgcga cgaccttgtt
     3241 gaccttcagc gccagcagcg cgagcagccc gcggacgccg ggaccgccga cggggacggg
     3301 ccgatcgccg tcgaggaggc gcacgggacc gaggagttgg aagcgggcag gggaaccgct
     3361 ttcggccatg acaagccccc ctccctccga tctctcccct taccggagta gccaacctac
     3421 cgcgcgaaga gcccgccaac cagaacgcgg caggtcgtga aggcctcctt ccctactttg
     3481 agggtaggga aggaggcctt cacggacgct cgatacggac aaatgcccca cggaggcttc
     3541 tgtccactat ggactcccca gggctggcgc gtgcgctcat gtcctgaagg cctcctttgg
     3601 gacgctgaac gtctcaaagg aggccttcag gacatgagcg atcagcgcca gggctcgcct
     3661 gaagtacatg aaggccccct tcctgtacct agacgcaagg aagggggcct tcatgtactt
     3721 ggcaagtccg tgaaggcctc cttgggggac ccagagtccc tcaaggaggc cttcacggac
     3781 cggacccgtg ccgctcaacg agacgaaaaa ggctccgtgc catcagtccc gatctgatgg
     3841 cacggagccg ggggatgtcc gacttgggcc gcctgggggc gcgacccaca tcggacatcg
     3901 gcgtccacgt ccgtccgggt gggctcaccc cgagcgaaga acccgttcgg tcgtcgacaa
     3961 cacctacgtg agcgagccca ctggcccgct cacgtggcga caacgtaaca gggcgcacac
     4021 cgacggctac aagcgccccg tcatggcctt cgcgacctgc ggtttcgtca aacgggtcag
     4081 cgcgtcagcg gggtcgagtc gcggccggcg atgaaggccg gacgcggttc gatcgcggcg
     4141 tacggcttct gcagcgcgtt ctcgacgctg ttgaacacga cgaagatgtt cgaccgcggg
     4201 tacggcgtga tgttgttcga ggaaccgtgc atcacgttcg aatcgaacca gagcgcggaa
     4261 ccggcctggc cggtgaactg gtcgattccg tattcggcgg cgagcgaagt gatgtcttcc
     4321 tcgctcggga cgccgacccg ctgctctttc agcgaactct tgtagttgtc ctccggggtc
     4381 tcccccgcgc actgcacgaa ggtccgctgc gaacccggca tcaccatcag cccgccgttg
     4441 aacgggtagt tgtcggtgag cgcgatggag cagctgaccg cgcgcggcag cggcatgccg
     4501 tcctcggcgt gccaggtctc gaagtccgag tgccagtaga acccggtgcc cttgaagccg
     4561 ggcatgtagt tcacgcggct ctggtggatg tacacctcgg agccgaggat ctgccgggcc
     4621 cggtcgagca cgcgcgggtc gcggacgagt tccgcgatca gctcgctggt ctcgtggacg
     4681 tcgaagatgg agcggacctc accggtcttg gcctcggtga tgacgcgctc gtcgtcgcgg
     4741 tggtcccccg acgacatcct gacgagttcc tgccagtacg tctggatttc gccaggcgac
     4801 aacaactggt cgacgacggt gtaaccacgc gtttcgtggt tggcgagcgt ggccgcgtcc
     4861 aaggggccgt cggcctcggt gccccagacg gtggggtgcg tcctgggcag gtgttcgggc
     4921 ttacccgtga tgcgggtcgg gtaactgtcg tcgactcggg tgtcggtcag cgtcaatgca
     4981 gtcgcctcct cgagcgtttt ctagggttcg gtgacgagcg ggtacacgcc gttttcgtcg
     5041 tggacctcac ggccggtgat gggcgggttg aacacgcaga cgcacttgat ctcggtcttg
     5101 ggccggacct ggtgcttgtc gtggtcgttg agcaggtaca acgtgcccgg cttcagctgg
     5161 aagacctcgc cggtcgcggt gttttcgagc tcgccttcac cggaggtgat gaacaccgct
     5221 tcgatgtggt tggcgtacca gaagtcgttg accgtcccgg cgtacaacgt ggtctcgtgc
     5281 accgagaaac cgacgccctc cttggccagc acgatgcgct tgctgcgcca gttcggggtc
     5341 ttgatgtcgg cgtcggtgtc ggtgacctcg tcgagtgttc tgacaagcaa cggaaaactc
     5401 cttctcgttc ctggttgagg gcggtcggtt cgggtcagtt gaggacggcg gcgacggcct
     5461 cgtcgatgat cgcgaggccc ttcgtgagct cgtcgtccgt cagggtgagc ggcgggagga
     5521 ccttcatgac ctctccgtcc ggcccggagg tctccatcag cagcccgcgc tcgaacgcgg
     5581 cggcgcagac cttgcccgcg aggtcgccgt tggcgaactc gatgccccgg gcgaggccgc
     5641 ggcccttcgc gaacaggttc gcgtccgggt acgcctcgac gatgccctgg aaggcggcga
     5701 cgacgcgctc gcccttggcc ttggtcgact tctccagctc gtcgtcgctc cagtagacgc
     5761 gcatcgcctc ggtcgcggtg acgaacgccg ggctgatgcc gcggaaggtg ccgttgtgct
     5821 cacccggctc ccagacgtcg aggtccgggc ggatcagcgt cagcgccatc gggatgccgt
     5881 agccgccgat ggacttcgag aggcagacga tgtcgggttt gatgcccgcg tcctcgaagc
     5941 tgaagaacgg gccggtgcgg ccgcagccca tctggacgtc gtcgaggatc agcaggatgc
     6001 cgtgcttctt gcacagatcg tccagcgcct tgagccactc gacgcgcgcg gcgttgatgc
     6061 cgccctcgcc ctgcacgcct tcgacgatca cggcggcggg ctcgttcagg ccgctgccgg
     6121 agtcttcgag cagcttctcg aagtagagga agtccgggat ggagccgtcg aagtagttgt
     6181 cgtacggcat cggggtcgcg tgcaccagcg ggacacccgc gccgccgcgc ttcatcgagt
     6241 tgccggtcac cgagagggcg cccagcgtca tcccgtggaa ggcgttggtg aagttgatga
     6301 ccgattcctt gccggtcacc ttgcgggcga gcttcagcgc ggcctcgacg gcgttcgcgc
     6361 caccggggcc ggggaagacg accttgtagt cgagatcgcg cggccgcagg atcttctcct
     6421 ggaacgtctg gaggaagtcc cgcttcgcca cggtgaacat gtcgagcgcg tgcgtcacgc
     6481 cgtcacgggc gatgtagtcg atcagcgccc gcttcagtgc cgggttgttg tgcccgtagt
     6541 tgagcgcgcc ggcgcccgcg aagaagtcga gataggcctt gccgtcttcg tcgtacaggt
     6601 agctcccctg ggcccggtcg aacaccacgg gccagccacg gctgtaactg cgtacttcgg
     6661 attcgagttc ctcgaaaatg ctcatcacgt tttctttctc cctgagatcc ccggatttac
     6721 gttctattga ctgctgagcg gaccgattcg gtacaggtcc tcctgctcat gcgttccttc
     6781 ctcggccggg aaatccgtcg cggagaacag atcctggcgt tcgacggtgg tgtcccaccg
     6841 cttggcgaac gaggcgaaca accggatcga agcctcgttg tccggcgtga tcgtggtttc
     6901 gaggtaccgc acgccctggg cggtcagacg gctgaacagc tcgtcgagca gggctccggc
     6961 caggcctttt ccccgctggg acgcgtcgac cgcgacctgc cacacgaagc cggtgtcctg
     7021 cttgcggtat ccgatcacga agccgaccgg gacgccgtcg actttcgcga cgaccgaggt
     7081 gtcggcgaaa tcgtggcacc acagcaaata ggcgtaaggc gtgttgagat cgagcttctt
     7141 cgaatcgcgg gcgattctcc agagcgcggc accgtctgcc ttggtcggag tttcgatcaa
     7201 gtgctttccg gacatgtcca agaaacgtac cggaggattc gggcgcggtc agatcagcgc
     7261 ggcagcggca acgcggctac ctgcaacaac ctcgaaacac acgtgataaa ggtaactgta
     7321 acgtttgaag cgcagcacgt gtgggtaagg ccgcttcggt gcggtctcac tgcacgtgac
     7381 gataccaacc gttaccactt cgtcgcacgg tcaggacgcg attcgggttc cgattccgct
     7441 attcacgacg tcgcttgcga cccgcgagcg aaccgatcgg cgtccagatg gcgggcagga
     7501 cgagcagcag gcagacgacc agccaccaca ccggcgcctc gccgcgccgc cagcccgcga
     7561 cgacgtcgcc gagccagccg aagagcgggt cgcgcaccgg tggcaggaac gcggcgaaca
     7621 gcagcgccgc caccaccagc acgccgagca ccgtgagcac catccgccac gtcttgcgca
     7681 acgtcatcag gctgaccacc aggaacacga cggcgcccgc ggcgaccggc acgccgaccc
     7741 actgcaggac cgggccgccc tgtccaccga ggacgaagcc cgccagcacg gtgatcagcg
     7801 cggcgagcac gttccacggc gacggcaggg tggccgcgcc gcgcgtgatc caccacgcgc
     7861 tgcgcccggc ggccttggcg aaggtcgcgg cgggctggac cgatttcggc acccgcgtcg
     7921 cccccgcggc cgcgttgagc ccggccgcgc ccgccttgac cagcgtcttc gtcaggagca
     7981 tcgtcccgcg ttcgtgctcg aaccgttcgc cggagacctg gcacgcctgg aacgcgcgct
     8041 gggtctgctc atcggtttcc ggctcgttct cgatcagccg tctgaaccgt tccgacggtt
     8101 ttccattgcc cttggccgtt ttcgcgtcgt gtccgcagtg gaggccgaca cacggcagtt
     8161 cctcgcgcgc gatctccagc tgccgcaccc tggcgagcac catcgccgtc accggcatgc
     8221 tgatcggcag ggcggcttcg gtctccacgt cggcgagttc gccgtcgaga ccgaggaaag
     8281 ccagttcccc ggccaattgc gcgcgcaggg cgtccgcttc ctcttgcgac agaccgtctt
     8341 ccgtgcccgg ctgtcgccag ccgatcgcct tgaacgtgtc ccgtacctgt gtccggaacg
     8401 cctcccgccc gacgacgtcg cgcagcagcc gcaggcgttt cgggtcgagc aggcattgca
     8461 ccagccagcc cgcgccgtcg acgcggcccc acatccaatc gttcgcgcgc caggacgcct
     8521 tgtagaacgc gccgaaatag tcggcctgca tcccggtgag cttgtcccag gaacggcggc
     8581 gcttcatgtc catcaatgtc cgcgaatccg cgctcacctg gacgagatcg acccgctggt
     8641 cgaccgacgg cggctgcgcg agcaatcctc gcgtcgcgat gtggagacgc accaggcgcg
     8701 aggtgacgac gggatctccg gcgctctcgt catcggacgt gagacctgtc caggcgagca
     8761 gcgtgctgac cgcgtcggcg gcttcggtgt cgggtttcgc ctgtgccagc ggcaaaagga
     8821 tcggcgtcgc ctgccggagc gcggcgacga cgagcggcca cgcctccttc agcgtgtccg
     8881 accggccgag ccccgcccat tccttggcca ggccttcgat ccaggcagcg agcgtgacat
     8941 cggccgacgg cgggccctgt tcggtgaccc agcccgcgag ccgtttcccc cgcgcggcct
     9001 tgtgacgcgc ttcgtgcaac agcacccggc aacgattgag ctgcaccgcc tgctccggcg
     9061 acggatcgag ccggaagccc gcgttcacca gctggaggcc ggtcgcgacg gaatcatcga
     9121 gcgcggtggt gcggaaggcg accagatccg tgagatcgca ggtgtccttc ggcggctcgg
     9181 tgggcaagcc gtcccgcaat ccggccgccg cggccgcgcg cagctgcgcc gccgtaccgg
     9241 acgcccactg gcggccttga tcctccacat cggacagtga gagcctgcgc gtcgcctcgc
     9301 ggaccagcgc ggtggcgtcc tcctgcaccc gccggtcgcg gtagtcgttc atcagccgct
     9361 ggtcgaccag cgtgtccggg ccgccgcgaa cccccatcgc ggccagggag accctggtcc
     9421 ccctggtgcg gacgacggcg tcgttgtgcc tggtcaggtc ttccagttcg gcactgatcg
     9481 tctggctcag cacggtgccg acgaccttgc tcatcgcact gcccagcagc ggcgggcgct
     9541 cgggatcgac cgcgacccgc tcggcctcgc gttccgccgt cggcaccacg tacagcagca
     9601 gccgccggac gtccgaatgg gactgccgtt cgaagatctc ccgcagcgcc ggccgcagcg
     9661 gtttgttgag cagcaccccg ccgtcggtga gccagtgcga tcgcgtgacg tcggtgtact
     9721 tcgtcatgtc cggatgcagc ggaccggtct tctcgccgat cggcatccgg gacagctcga
     9781 acgcgccggg gaacgacgcg gtggaccgcg ccgccaacgc gagcggcccg accacgtcgt
     9841 ccgtccacaa aggagcgtcg aagcggaaca gcaggcgatg ttcggtgtcc cgcaccaggt
     9901 ttccgagcgc gtcgtcgaac cgggtcgtct cgccgtcgat catcgtgccg gtgagcagca
     9961 cggtgatgtc gggacagtcg ggtttcaccg tcgccttgtc ggtgatccgg tgcaacgcgt
    10021 ccttgagatc cccgagcagc accttgtcgc cgtcgagcag cgaacgtggt tccttctcgc
    10081 cgggatcgcg gatcaggttg tcgagcgatc ccgtgctgat ccacgtgtcg cgcagcacct
    10141 gtgggctcga ccggaacgcc tcggccagcc cgaggcacgc cgcgttgatg ccgcccgcgc
    10201 tcgtgccggt gagcacgtcg agcgagacgg tggcgttgag caggtcgagc agggcccggt
    10261 acgggctctt cgattccggc gctctcgacg cctgcaacag atgcgaggtc tcggtggcga
    10321 ccccgcccat ccagaccgcg agactcgccc cgcccaccat ggtcatcgcc agccggatct
    10381 cctgcggcca
//
