LOCUS       NZ_LIVW01000008        10381 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02115 scaffold8, whole genome shotgun sequence.
ACCESSION   NZ_LIVW01000008
VERSION     NZ_LIVW01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02115
  ORGANISM  Streptomyces sp. CB02115
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 466474)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466474)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905645.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,651
            CDSs (total)                      :: 7,572
            Genes (coding)                    :: 7,475
            CDSs (with protein)               :: 7,475
            Genes (RNA)                       :: 79
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 79 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 337855
            Orig. end                         :: 348236
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVW01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10381
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10381
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10381
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5381](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5381)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(225..240)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.44"
                     /tool="antismash"
     gene            531..1355
                     /gene="griI"
                     /locus_tag="AMK28_RS33695"
                     /old_locus_tag="AMK28_33220"
     CDS             531..1355
                     /EC_number="4.1.2.56"
                     /codon_start=1
                     /gene="griI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380462.1"
                     /locus_tag="AMK28_RS33695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33220"
                     /product="2-amino-4,
                     5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate synthase"
                     /protein_id="WP_073746113.1"
                     /transl_table=11
                     /translation="MAPNAPFARSLRLQRLHHHDPERLFIVPLDHSVTDGPLTRVHRLD
                     SLVGELASHHVDGIVLHKGSLRHVDPEWFTRTSLIVHLSASTVHAPDPNAKYLVSSVEE
                     SLRMGADAVSVHVNLGSDEERHQIADMAAVAEACDRWNVPLLAMMYPRGPRIDDPRDPA
                     LVAHAVQVAVDLGADLVKTLYVGSVAAMAEITASSPVPVVVVGGPRDSDESRVLAYVED
                     ALRGGAAGVAMGRNVFQADDPGAMADKLSDLIHHSGGSSAARAPAGAAAGAA"
     gene            1456..2586
                     /gene="griH"
                     /locus_tag="AMK28_RS33700"
                     /old_locus_tag="AMK28_33225"
     CDS             1456..2586
                     /EC_number="4.1.99.20"
                     /codon_start=1
                     /gene="griH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380461.1"
                     /locus_tag="AMK28_RS33700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33225"
                     /product="3-amino-4-hydroxybenzoic acid synthase"
                     /protein_id="WP_073746114.1"
                     /transl_table=11
                     /translation="MPSSSSSSSKLTWLDIRSVGEARAAIVQEALHHRVEALVADDPAH
                     LADLPPTVTKVLLVLGKQVPEEFGEATVVVVDPSKHGVTPAELAIKHPEIEFGRFVEII
                     DAPTLEDACESSRTEKWSVLLFRDPTKIPLEIVIAAAARATGSMVTVAQDLEEAEILFG
                     VLEHGSDGVMMAPRAVGDAAELKRIAEAGIPDLDLTELRIVETSHIGMGERACVDTTTH
                     FGEDEGILVGSHSKGLILCVSETHPLPYMPTRPFRVNAGAIHSYTLGKDERTNYLSELK
                     TGSKLTAVDVKGNTRLVTVGRVKIETRPLISIDAEAPDGRRVNLILQDDWHVRVLGPGG
                     TVLNSTELKPGDRVLGHLPVEDRHVGYPINEFCLEK"
     gene            complement(2561..3919)
                     /locus_tag="AMK28_RS33705"
                     /old_locus_tag="AMK28_33230"
     CDS             complement(2561..3919)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380460.1"
                     /locus_tag="AMK28_RS33705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33230"
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="WP_073746115.1"
                     /transl_table=11
                     /translation="MYVVLALCWLVVFFDGLDVTVYGAVMPYMLDDTAFGLDAPAAGRI
                     GSWTTFGMLIGALTAGNITDWIGRRTVLVWCVLLFSAGSGVCALAGDPVQFGAGRFLAG
                     LGLGGLMPICLTVVAEFSPPRRMALATGVLMTAYHAGGMAATGLGLALAPDHGWRWSFA
                     AGVLPALVAVPLLLRYLPESPGVLYARGRFEQARGTAERYGLPEPSAEDAPGAGASGRL
                     RAVRDLVAPGRAPATLLLWAASFCGLLLVYGISTWLPQLMRSAGYGLSTSVGLLMVINA
                     GGIVGMLVAGRVADRFGAVRVATCWFLLTAAGLALLATGPSLGLTYAVVAFTGVWLFSA
                     STMVYAVAGRFYPAADRAAGLGWVTGVGRLGAVVSPMLGGALVGRGLGSGGFLVFALGG
                     VLGAVTVFLVPVVATARTRTGAGRAAGKEPGDAAGAGREPGSRVGPTSPGRTR"
     gene            complement(4041..4961)
                     /gene="griF"
                     /locus_tag="AMK28_RS33710"
                     /old_locus_tag="AMK28_33235"
     CDS             complement(4041..4961)
                     /EC_number="1.10.3.15"
                     /codon_start=1
                     /gene="griF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380459.1"
                     /locus_tag="AMK28_RS33710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33235"
                     /product="grixazone synthase"
                     /protein_id="WP_073746117.1"
                     /transl_table=11
                     /translation="MVHVRKNHLNMTAEEKRRFVNAVLEIKRRGVYDRFVKLHIQVNST
                     DYLDKETGKRLGHVNPGFLPWHRQYLLKFEQALQKVDPRVTLPYWDWTTDHGENSPLWS
                     KEFMGGNGRPGDRRVMTGPFARRNGWKLNISVIPEGPEDPALNGNYTHDDRDYLVRDFG
                     TLTPDLPTPQELEQTLDLTVYDCPPWNHTSGGTPPYDSFRNHLEGYTKFAWEPRLGKLH
                     GAAHVWTGGHMMYIGSPNDPVFFLNHCMIDRCWALWQARHPDVPHYLPTVPTQDVPDIN
                     TPLGPWHTKTPADLLDHTQFYTYDQ"
     misc_feature    4922..4936
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.34"
                     /tool="antismash"
     gene            complement(5001..5381)
                     /locus_tag="AMK28_RS33715"
                     /old_locus_tag="AMK28_33240"
     CDS             complement(5001..5381)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380458.1"
                     /locus_tag="AMK28_RS33715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33240"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_241837662.1"
                     /sec_met_domain="melC (E-value: 9.2e-34, bitscore: 111.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRREMVMATTGAALVAAAAAVPLLSGGDGEKATEAAAEPAKATG
                     REHTERYLGRTIRVATPADGGGVFIDGRPLHLMKFADDAYLSSMCHYEMAPTPLHAARR
                     AVEELRGAALQPSTHGTHVTHL"
     misc_feature    complement(5377..5390)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.62"
                     /tool="antismash"
     gene            complement(5443..6813)
                     /locus_tag="AMK28_RS33720"
                     /old_locus_tag="AMK28_33245"
     CDS             complement(5443..6813)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515807.1"
                     /locus_tag="AMK28_RS33720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33245"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_073746119.1"
                     /sec_met_domain="Aldedh (E-value: 6.5e-15, bitscore: 49.2,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTVAPPAVVLDPRVCGRRVASSDRTPLPSVLGGDLADIGTAPRL
                     LAVAALNEIRAHADGRPPAFEVFTEAARLFADATLDGESPQEYARRVCHATGLTATAVE
                     QAVADLTGELRALPATTAAELPATGFGEGFDTRWVPRGRVFVAVMASNHPVPNISWAQA
                     LFHGYSVLVKPGSRDPFTPARLLAALIAAGLPPARAAFLPCSREVGEYLLREADRGIIY
                     GGESAVATWHQRESVAVRGPGRTKALLDGAPDDAVIDHLALSASFDGGTRCTNLSAVLT
                     TGPVEEVADRLAERLAKLPSLPATDDGATLLVVSRARAERLREQVAELRASLTDHSARA
                     GEHETVVELADGSFLLRPVVLSAGRADHPAVGTELPFPFVVVAPWTEADGVEPLRDSLV
                     LNLLTDREDLVEAAVREPSVRKVTRGRVLPWTATPGIPHDDNYTQFLLEPKGVVARR"
     gene            complement(6813..7871)
                     /locus_tag="AMK28_RS33725"
                     /old_locus_tag="AMK28_33250"
     CDS             complement(6813..7871)
                     /NRPS_PKS="Domain: AMP-binding (80-301). E-value: 5.1e-12.
                     Score: 37.0. Matches aSDomain:
                     nrpspksdomains_AMK28_RS33725_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380456.1"
                     /locus_tag="AMK28_RS33725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33250"
                     /product="AMP-binding protein"
                     /protein_id="WP_073746120.1"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-07, bitscore:
                     25.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHIVDPEFDSHVVKLMAWHFGAETGSPFWLEKLDGLGFDPLSEV
                     RGLADLTRFPDVSAELRSVPAQDLIPRGLSDRPFRVYDSGGTTGSPKRIVDSAYRSLVT
                     GWARDRLIASGVPAHGNWLHLGPGGPHVIGFDTARYAALGGGVFYTVDLDPRWVKRLLS
                     QGRGEEADAYIQHLLDQAETILESQPVAVLNTTPPLLEAICARPRLYELVRTEVRAIIW
                     AGTSISRESLKQLGDVFFPAAHVVGVYGNSLMGVAPQRPVRSDDAHPCVFEPFPETTRL
                     HLVDEDGEPVAYGERGRVRLHLVSEEMFLPNILERDTAIRVEPSDGSAVDGLADVQTYR
                     SLDDVAIIEGVY"
     aSDomain        complement(6969..7631)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK28_RS33725_AMP-binding.1"
                     /evalue="5.10E-12"
                     /label="AMK28_RS33725_AMP-binding.1"
                     /locus_tag="AMK28_RS33725"
                     /protein_end="301"
                     /protein_start="80"
                     /score="37.0"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="VYDSGGTTGSPKRIVDSAYRSLVTGWARDRLIASGVPAHGNWLHL
                     GPGGPHVIGFDTARYAALGGGVFYTVDLDPRWVKRLLSQGRGEEADAYIQHLLDQAETI
                     LESQPVAVLNTTPPLLEAICARPRLYELVRTEVRAIIWAGTSISRESLKQLGDVFFPAA
                     HVVGVYGNSLMGVAPQRPVRSDDAHPCVFEPFPETTRLHLVDEDGEPVAYGERGRVRL"
     gene            complement(8047..9399)
                     /locus_tag="AMK28_RS33730"
                     /old_locus_tag="AMK28_33255"
     CDS             complement(8047..9399)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968442.1"
                     /locus_tag="AMK28_RS33730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_33255"
                     /product="MFS transporter"
                     /protein_id="WP_073746270.1"
                     /transl_table=11
                     /translation="MTTHEAASTAAAGSVSSPGARSDGLRYGALFGPVVFGVTAAGVAL
                     PDVASSLDASSTATAWVLTAHALALGVGTALFGRLADTRGVRAALLLGSLALAAGAVVC
                     LLAPNLGVLVAGRFVQAAGSGAMAACAMALTASVPPERRPQVFAGFGATMAIFSASATL
                     AGGVFTEWVTWRIALVLPALSLLIVPLCLAGAPVRKNSGRSMDLPGAALLTLTAMSFLV
                     LIQSSALALSTPLVIATAAVLVLSAAGLTWRTRGSESSFVPRALVTDRVFLKAAVIGIG
                     VYGGLFAAMYAVPQLLVRDHGWSVLSVGVWLLPGAVVGAVLSRLAGRLTAGDHGNRLLG
                     AVSLAFAAALGGALTGSGVLLLILGASLGFSAFSVTQVFTTALMSGHIEPARRGGAMGL
                     LNLTFFVGGGVGSATAGALAKSMSLSTALGVVALFPLIAALVALTLGRRTP"
     misc_feature    9900..9917
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.59"
                     /tool="antismash"
ORIGIN
        1 cgacatcgcc cggatcaccc tgcgcaccca cgacaccccg gacccctccc ccgccgtctt
       61 ccggttcctg gcggaggagt ccattcccgc cgacatgacc acggtctcct ccgcgcccgg
      121 cggcggcttc tcgctcggcc tcacggtgca ccgctcgcac gcggcggacg tcagccggtc
      181 gttcagcgat ctcatggccg cgcggcggca cggagtcgag gtcgaggacc aggtcgccaa
      241 ggtctcgatc gtcggtcagg gcctgctcag ccggcccgag tacgcgtccc ggatgctcgc
      301 ctcgctcgtc ggctccggca tcagcgcccg gtccctgtcc gcgtcccagc tgcgggtctc
      361 ggtgacggtg ccccgctgcg acgcggtacg cgcggtgggc ctgctgcaca gcgagttcgg
      421 gctcggcgcc gggaccggcg cggtccccgc acgcctctcc ccccggccct gatcccggtc
      481 cgagacggcg tacgcgacac ctgctgcgcc gacacccgac aggagcaccg atggccccga
      541 acgcgccctt cgccaggagt ctgcgacttc aacggctcca tcaccacgac cccgagcggc
      601 tgttcatcgt cccgctcgac cactccgtca ccgacggtcc gctgacccgc gtccaccggc
      661 tggactcgct cgtcggcgag ctggcgtcgc accacgtcga cgggatcgtc ctgcacaagg
      721 gctcgctgcg ccacgtggac ccggaatggt tcacccggac atccctgatc gtgcacctga
      781 gcgccagcac cgtgcacgcc cccgacccga acgccaagta cctggtgtcg agcgtcgagg
      841 agagcctgcg catgggcgcg gacgcggtga gcgtccacgt caatctcggc tccgacgagg
      901 aacgccacca gatcgcggac atggcggcgg tcgcggaggc ctgcgaccgc tggaacgtac
      961 cgctgctggc gatgatgtat ccgcgcggcc ccaggatcga cgatcctcgc gatccggcgc
     1021 tcgtcgccca tgccgtccag gtggccgtcg atctcggcgc ggacctggtc aagacgctgt
     1081 acgtcggatc ggtcgcggcg atggccgaga tcaccgcgtc ctcgccggtt ccggtcgtcg
     1141 tggtcggcgg gccgcgcgac agcgacgaga gccgggtcct cgcctacgtc gaggacgcgc
     1201 tgcgcggcgg cgcggccggt gtcgccatgg gccgcaacgt cttccaggcc gacgatccgg
     1261 gcgcgatggc ggacaagctc tccgacctca tccaccacag cggcggcagc tccgcggcgc
     1321 gggctccggc cggcgcggcg gccggagccg cttgatccgg ccgacgtccc cgcccgggtc
     1381 ctcgcccgtc acaccacccg gtcccagcac cccgtcccag ctctcaccgt cccatcgcta
     1441 ctgaggagtt cctccatgcc ttcgtcctcg tcctcgtcct cgaagctgac ctggctcgac
     1501 atccgctccg tgggtgaggc ccgtgccgcc atcgtccagg aagccctgca ccaccgggtg
     1561 gaagcgctgg tcgccgacga tcccgcccac ctcgcggacc tgccgcccac cgtgaccaag
     1621 gtcctgctcg tcctgggaaa gcaggtcccg gaggagttcg gcgaggcgac ggtcgtcgtc
     1681 gtcgacccgt cgaagcacgg tgtgacaccg gccgaactgg ccatcaagca cccggagatc
     1741 gagttcggac ggttcgtgga gatcatcgac gcgccgacgc tggaggacgc ctgcgagtcc
     1801 tcgcggaccg agaagtggtc cgtcctgctg ttccgcgacc cgaccaagat cccgctggag
     1861 atcgtgatcg ccgccgccgc acgggccacc ggttcgatgg tgaccgtcgc gcaggacctg
     1921 gaggaggcgg agatcctctt cggagtgctg gaacacggct cggacggcgt gatgatggcc
     1981 ccgagggccg tcggcgacgc cgccgagctg aagcggatcg ccgaggccgg tattcccgac
     2041 ctcgacctca ccgagctgcg catcgtggag accagtcaca tcggcatggg cgagcgggcc
     2101 tgtgtggaca ccaccacgca cttcggcgag gacgagggca tcctggtcgg ctcgcactcc
     2161 aagggcctga tcctctgcgt cagcgagacc cacccgctgc cgtacatgcc gacccggccg
     2221 ttccgcgtca acgccggcgc cattcactcg tacacgctgg gcaaggacga gcgcaccaac
     2281 tacctcagcg aactgaagac gggcagcaag ctcaccgccg tcgacgtcaa gggcaacacc
     2341 cggctggtga ccgtgggccg cgtgaagatc gagacccgcc cgctgatctc catcgacgcc
     2401 gaggcaccgg acggacggcg cgtcaacctg atcctccagg acgactggca cgtccgggtc
     2461 ctcggtcccg ggggcacggt cctcaacagc accgagctga agcccggcga cagggtcctc
     2521 ggccacctgc ccgtcgagga ccgccacgtc ggctacccga tcaacgagtt ctgcctggag
     2581 aagtaggccc cacccgggag cccggctccc gcccggctcc ggccgcgtcg cccggttcct
     2641 tcccggcggc gcggccggcg cccgtacgcg tacgggccgt cgcgacgacg ggcaccagga
     2701 acacggtgac ggctccgagg accccgccga gggcgaagac caggaacccg ccgctcccca
     2761 gcccccggcc gaccagcgct ccgccgagca tcggactgac caccgcgccg agccggccca
     2821 ccccggtgac ccagcccagg cccgcggcgc ggtcggcggc ggggtagaag cgtccggcga
     2881 ccgcgtagac catggtgctg gcgctgaaga gccagacgcc ggtgaaggcg acgaccgcgt
     2941 aggtgaggcc cagcgacggg ccggtggcca gcagggccag cccggccgcc gtcagaagga
     3001 accagcaggt cgcgacccgg accgcgccga accggtcggc cacccgtccg gcgaccagca
     3061 tgccgacgat gccgcccgcg ttgatgacca tgagcaggcc gaccgaggtg gacaggccgt
     3121 aaccggcgga gcgcatcagc tgcggcagcc aggtggagat gccgtacacg agcagcagcc
     3181 cgcagaacga ggcggcccac agcagcagcg tcgccggtgc acggcccggc gccacgaggt
     3241 cgcgcaccgc gcgcagccgg cccgaggctc cggcgcccgg cgcgtcctcg gcggacggct
     3301 ccggcagtcc gtaccgctcg gccgtcccgc gtgcctgctc gaaccggccg cgggcgtaca
     3361 gcacgcccgg cgactccggc aggtaacgca gcagcagtgg tacggcgacg agggcgggca
     3421 gcacgcccgc cgcgaaggac caccgccagc cgtggtccgg cgcgagcgcg aggccgaggc
     3481 ccgtcgccgc catgccgccc gcgtggtagg cggtcatcag gactccggtg gccagggcca
     3541 tccggcgcgg cggcgagaac tccgccacca cggtcaggca gatcggcatc aggccgccga
     3601 ggccgagccc ggccaggaac cggcccgctc cgaactggac cggatctccg gccagcgcac
     3661 agacgccgga tccggcggag aacagcagga cgcaccagac caggacggtg cgtctgccga
     3721 tccagtcggt gatgttgccg gcggtcagcg cgccgatgag catgccgaac gtggtccagc
     3781 tgccgatccg gccggccgcc ggggcgtcga gcccgaaggc ggtgtcgtcg agcatgtacg
     3841 gcatgacggc gccgtagacc gtgacgtcca gtccgtcgaa gaagacgacg agccagcaca
     3901 gtgcgagtac tacgtacacg gagtctctcc cgggtcgatg cggtggaggg ccgaggcgga
     3961 ggaggtacgc acgaagcggc ggcgccggac agcggtgatc cgctgtccgg cgccgccgcg
     4021 tgggtccggg gcgagggccc ctactggtcg taggtgtaga actgcgtgtg gtcgagcagg
     4081 tccgccggcg tcttggtgtg ccagggcccg agcggggtgt tgatgtcggg cacgtcctgg
     4141 gtcggcacgg tcggcaggta gtgcggcacg tcggggtggc gcgcctgcca cagggcccag
     4201 cagcggtcga tcatgcagtg gttgaggaag aacaccgggt cgttcggcga gccgatgtac
     4261 atcatgtggc ccccggtcca cacgtgcgcg gccccgtgca gcttgccgag cctcggctcc
     4321 caggcgaact tggtgtagcc ctccaggtgg ttgcggaagc tgtcgtacgg cggtgtgccg
     4381 cccgaggtgt ggttccacgg cgggcagtcg tagaccgtga ggtcgagcgt ctgctccagc
     4441 tcctgcggtg tcggcaggtc cggtgtgagt gtgccgaagt cccgtacgag atagtcacgg
     4501 tcgtcgtgcg tgtagttgcc gttcagggcc gggtcctccg ggccctccgg gatgacgctg
     4561 atgttcagct tccagccgtt gcggcgcgcg aaggggccgg tcatcacgcg gcggtcgccg
     4621 ggccggccgt tgccgcccat gaactccttc gaccacaggg gggagttctc gccgtggtcg
     4681 gtggtccagt cccagtacgg gagggtgacg cgcggatcca ccttctgcag ggcctgttcg
     4741 aacttcagca ggtactgccg gtgccacggc aggaagcccg ggttgacgtg gccgaggcgc
     4801 ttgccggtct ccttgtcgag gtagtcggtg gagttgacct ggatgtggag cttgacgaac
     4861 cggtcgtaga cgccgcgtct cttgatctcc aggaccgcgt tgacgaaccg gcgcttctcc
     4921 tcggccgtca tgttcaggtg gttcttgcgt acgtggacca tcgctgggct tccttcgctg
     4981 cggggccggt tcaaaggtgg tcagaggtgc gtgacgtggg tgccgtgcgt ggagggctgg
     5041 agtgcggcgc cgcgcagttc ctcgacggct cgccgggcgg cgtgcagcgg ggtgggggcc
     5101 atctcgtagt ggcacatgga gctgaggtag gcgtcgtcgg cgaacttcat gaggtggagc
     5161 ggccggccgt cgatgaagac gcctccgccg tccgccggag tggcgacgcg tatggtccgc
     5221 ccgaggtagc gctcggtgtg ttcccggccc gtggccttcg ccggttcggc ggcggcctcg
     5281 gtggccttct cgccgtcgcc gccgctcagc agggggacgg cggccgcggc ggcgacgagc
     5341 gccgctccgg tggtggccat gaccatttct cgcctgttca tgggcatgcg aactgccttt
     5401 ctcacggtct cgtgcatgtc ggaggcgggt cggcgggagc cgtcaacgcc gggccacgac
     5461 gcccttgggc tccaggagga actgcgtgta gttgtcgtcg tgcgggattc cgggcgtcgc
     5521 ggtccacggc aggacccggc cgcgcgtcac cttgcggacg ctcggctccc ggacggcggc
     5581 ctccaccagg tcctcgcggt cggtgaggag gttgaggacg agtgagtcgc ggagcggctc
     5641 cacgccgtcg gcctccgtcc acggggccac cacgacgaac gggaacggca gttccgttcc
     5701 gaccgccgga tggtcggcgc ggccggcgga gagcacgacc ggtcgcagca ggaacgaacc
     5761 gtcggccagc tccacgacgg tctcgtgctc tccggcgcgc gcgctgtggt ccgtgagcga
     5821 ggcccggagc tcggcgacct gttcgcgcag ccgctcggcg cgggcccggc tcaccaccag
     5881 gagcgtcgcc ccgtcgtcgg tcgcgggcag cgagggcagc ttcgcgagcc gctccgcgag
     5941 ccggtcggcg acctcctcga ccggtccggt ggtcaggacc gccgagaggt tggtgcagcg
     6001 ggtgccgccg tcgaaggagg cggagagcgc caggtggtcg atgacggcgt cgtccggggc
     6061 cccgtccagc agcgccttgg tccggccggg accgcgcacg gccaccgatt cccgctggtg
     6121 ccaggtggcg acggcgctct caccgccgta gatgatgccc cggtcggcct cccgcagcag
     6181 gtattcgccg acctcgcgcg agcagggcag gaacgcggcc ctggccggcg gcaggcccgc
     6241 cgcgatcagc gcggcgagca gccgggccgg ggtgaacggg tcgcggctgc cgggcttgac
     6301 gaggacgctg tagccgtgga acagcgcctg cgcccaggag atgttcggca ccgggtggtt
     6361 gctcgccatc accgcgacga agacccttcc gcgcggcacc caacgggtgt cgaagccctc
     6421 gccgaagccg gtggccggca gttctgcggc ggtggtcgcc gggagcgcgc gcagttcgcc
     6481 ggtcaggtcg gcgacggcct gttcgacggc ggtggcggtg aggccggtgg cgtggcagac
     6541 ccgcctggcg tactcctgcg ggctctcgcc gtccagcgtc gcgtcggcga agagccgggc
     6601 cgcctcggtg aacacctcga atgcgggcgg tcggccgtcc gcgtgtgcgc ggatctcgtt
     6661 cagcgcggcg accgcgagca gccggggcgc ggtcccgatg tcggcgaggt cgccgcccag
     6721 cacgctcggc agcggggtgc ggtcggagct ggcgacgcgg cggccgcaga cgcggggatc
     6781 gaggacgacg gcggggggcg cgaccgtgct catcagtaga ccccttcgat gatggccacg
     6841 tcgtcgaggc tgcggtaggt ctgcacatcg gcgaggccgt ccacggcgga gccgtcgctc
     6901 ggctcgacgc gtatcgcggt gtcccgttcc aggatgttcg gcaggaacat ctcctccgag
     6961 accaggtgga gccggacccg gccgcgttcg ccgtaggcca ccggttcgcc gtcctcgtcc
     7021 acgaggtgga ggcgcgtggt ctcggggaac ggctcgaaga cgcacgggtg ggcgtcgtcg
     7081 gaccggaccg gccgctgcgg cgccacgccc atcaggctgt tgccgtagac gccgaccacg
     7141 tgcgccgccg ggaagaagac gtcgccgagc tgcttcaggc tctcccggct gatggacgtc
     7201 ccggcccaga tgatcgcgcg gacctcggtg cgcaccagct cgtacaggcg cggcctggcg
     7261 cagatcgcct ccaggagcgg cggagtggtg ttgagcaccg cgacgggctg gctctccagg
     7321 atcgtctccg cctggtccag caggtgctgg atgtacgcgt cggcctcctc gccgcgtccc
     7381 tgcgacagga gccgcttcac ccagcgcggg tcgaggtcga cggtgtagaa gacgccgccg
     7441 ccgagcgcgg cgtagcgggc cgtgtcgaag ccgatgacat gggggccgcc gggccccagg
     7501 tgcagccagt tgccgtgcgc ggggacgccg ctcgcgatca ggcggtcccg cgcccatccg
     7561 gtcaccagcg accggtaggc gctgtccacg atgcgcttgg gcgatccggt ggtgccgccc
     7621 gagtcgtaga cccggaaggg acggtcggac agtcctcgcg ggatcaggtc ctgggccggg
     7681 acggagcgga gttcggcgct gacgtccggg aagcgggtca ggtcggccag gccccgcacc
     7741 tcggagaggg gatcgaaccc gagcccgtcc agcttctcca gccagaacgg cgagccggtc
     7801 tccgcaccga agtgccatgc catcaactta actacgtgac tgtcgaattc gggatcaacg
     7861 atgtggctca tgacggactt ccgctctcct gatcacgggg caagggcggt acggcaccca
     7921 tcgtgctggc gcggcctatc ggccccgtat cgcctgtgtg tcgtggaacg cggccgcccg
     7981 cacggcaccg ttcggacggt gctgcgcggg cggagggttc gtgcgggcgg agaggtcctg
     8041 cggcggtcag ggcgtgcgcc ggcccagggt gagggcgacc agcgcggcga tcagcgggaa
     8101 gagcgccacc acgccgagcg ccgtggagag cgacatcgac ttcgccagtg cgccggcggt
     8161 ggcgctgccg acgccgccgc cgacgaagaa ggtgaggttg agcagcccca tcgcaccgcc
     8221 gcgccgggcc ggttcgatgt gcccggacat cagcgcggtg gtgaagacct gcgtgacgga
     8281 gaacgcggag aagccgagcg aggcgccgag gatcagcagc agtacgccgg agccggtcag
     8341 cgcgccgccg agggccgcgg cgaaggcgag cgagaccgcg ccgaggagcc ggttgccgtg
     8401 gtcgcccgcg gtgagccggc cggccagcct ggagagcacc gcgccgacga ccgcgccggg
     8461 cagcagccag acgccgacgg acaggacgct ccagccgtgg tcgcggacga gtagctgcgg
     8521 tacggcgtac atcgcggcga acaggccgcc gtacacgccg atgccgatga cggcggcctt
     8581 gaggaacacc cggtcggtga cgagcgcgcg cggtacgaag gagctctcgc tgccgcgcgt
     8641 acgccaggtg agcccggcgg ccgagaggac cagcacggcg gcggtcgcga tcaccagcgg
     8701 agtgctcagc gcgagcgcgg aggactggat cagcacgagg aacgacatgg cggtgagcgt
     8761 cagcagagcc gcgcccggca ggtccatcga gcggccggag ttcttgcgca ccggtgctcc
     8821 ggccaggcac aggggaacga tgagcaggga gagcgcgggc aggaccagag cgatgcgcca
     8881 ggtcacccac tccgtgaaga cgccgcccgc cagggtggcg ctggccgaga agatcgccat
     8941 cgtcgccccg aatccggcga agacctgcgg ccgccgttcc ggcggcaccg aggcggtgag
     9001 cgccatggcg caggccgcca tggcgcctga cccggcggcc tggacgaagc gtccggccac
     9061 caggacgccg aggttgggcg cgagaaggca gacgacggcg ccggccgcga gggccagcga
     9121 gccgagcagc agtgcggcgc gtacgcctcg ggtgtcggcg aggcgtccga acagcgcggt
     9181 gccgacgccg agagcgagcg cgtgggccgt caggacccag gccgtggcgg tggacgaggc
     9241 gtcgagtgag gacgccacgt cgggaagggc gacgccggcg gcggtgacac cgaagacgac
     9301 cgggccgaac agggcgccgt agcgcagccc gtccgagcgg gctcccggag aggagacgga
     9361 accggcggcc gcggtcgacg cggcctcgtg cgtggtcatg atgaactcca aggattgacg
     9421 agaacctgcc aaaagacgag cggtccggga gcccgcatcc accggagcac gaagggccgt
     9481 tcggcgtgac gggcgaacag cgaccgcctt acggaagagg cgcgatgttg tggttgagcc
     9541 ggaagaggtt gtcggggtcg accgccgcct tcacgcgcgc cagccggtcc agggtctccg
     9601 gatcgaagca ctccgccgtc gagtcggccg tgtccgcgcc cgcgaggaag ttcagatacc
     9661 ggcggcccgt gctccacggc tccatcgggc cgaggaccac gtcgggcgag cccggaggcg
     9721 agagcgtcgt gagtgtgaac gcgccttcgc ggacgccgac cgcgttgggc acctcggcgg
     9781 gccgggagag cgcgccgccg aggtgccgca cctcgaccat caggtcgccg caggacgaac
     9841 ccgctccggc cacccgcagc agcgcctcca tggcgtccga tcccaactcc cgcagcacga
     9901 tgttgcgttc gtggtacgcg agcggatgca ccgggtcctt gtggatgtcg gccaccgcgg
     9961 tgtacggcat ctcggcgacc ccgtccacca gcggcgtacc gcactcgcgc agcggacgga
    10021 cgagccgctc cccctcctcg gcccggccgg tgaacgcgat ccgcacatgc gcggcgaacg
    10081 tcccgcggag gaactccggc acgtccggca cgtccggcaa ccgcagcaac gacacggagg
    10141 aggtcatctc ctcgggcacg gtcgcggtcc actgccgcca ggtgtgcagg acctgcgccg
    10201 cctgctcgcc ggggaagtag atcccgccgc cgtagagccg gcgcaccggc atcaggccga
    10261 accagagccc ggtgacgatg ccgaagttcc ccttgccgcc gcgcagcgcc cagaacaggt
    10321 ccggatgccg gtcgcgggtg acctgccgga cgccgccgtc ggcggtgacc acctcgatac
    10381 a
//
