LOCUS       NZ_LIVW01000005        10945 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02115 scaffold5, whole genome shotgun sequence.
ACCESSION   NZ_LIVW01000005
VERSION     NZ_LIVW01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02115
  ORGANISM  Streptomyces sp. CB02115
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 630329)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630329)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905645.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,651
            CDSs (total)                      :: 7,572
            Genes (coding)                    :: 7,475
            CDSs (with protein)               :: 7,475
            Genes (RNA)                       :: 79
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 79 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 175790
            Orig. end                         :: 186735
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVW01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5945)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    30..44
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(628..771)
                     /locus_tag="AMK28_RS39445"
     CDS             complement(628..771)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMK28_RS39445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158073352.1"
                     /transl_table=11
                     /translation="MGRTGPANHRPPHPVAGLILDAAQAEVLAARAALRAALSGPARAG
                     GS"
     gene            complement(1043..2083)
                     /locus_tag="AMK28_RS25910"
                     /old_locus_tag="AMK28_25530"
     CDS             complement(1043..2083)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-268). E-value: 4.5e-12.
                     Score: 37.6. Matches aSDomain:
                     nrpspksdomains_AMK28_RS25910_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="AMK28_RS25910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25530"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_073743672.1"
                     /sec_met_domain="ADH_N (E-value: 5.4e-29, bitscore: 95.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 3.9e-23, bitscore:
                     77.0, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDILIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGSCDACLMGR
                     EQHCAEGNTQTYNALDRSGEPTHGGYSTHIVVAETFALSIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     HATSDPRTFEELAGTFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPVSLNLFSL
                     ILGNKSIAGSAIGGIKETQEMLDFCAVHGLGAEIEVIDAGQVNEAYERILASDVRYRFV
                     IDTATI"
     aSDomain        complement(1280..2026)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK28_RS25910_PKS_ER.1"
                     /evalue="4.50E-12"
                     /label="AMK28_RS25910_PKS_ER.1"
                     /locus_tag="AMK28_RS25910"
                     /protein_end="268"
                     /protein_start="19"
                     /score="37.6"
                     /tool="antismash"
                     /translation="TIERRPVGEFDILIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGSCDACLMGREQHCAEGNTQTYNALDRSG
                     EPTHGGYSTHIVVAETFALSIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYHATSDPRTFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVG"
     misc_feature    2146..2161
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            2246..3172
                     /locus_tag="AMK28_RS25915"
                     /old_locus_tag="AMK28_25535"
     CDS             2246..3172
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="AMK28_RS25915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25535"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_073743674.1"
                     /transl_table=11
                     /translation="MDNQPEPAPEPLDRRAELSEFLRTRRAKLRPGDVGLPEFGRHRRV
                     PGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAESAHLTHLAKPA
                     RHKKKRRPARVQRVRKGLLYLLDSMDGIPAYVTGARSDVLAWNAMAAAVFGDWGALPPA
                     ERNWARLVFLSPAYRDLFVNWDSKASDMVSYLRLYAGCHPDDPELSALVGELSVKSDEF
                     RRLWATHNVKEKGHGVKLLRHPLVGELTLSYETLNLPDDEEQHLVTYHAEPGSESAQSL
                     RLLASWGADAGLTDVIGDAGRGPADER"
     gene            complement(3132..3878)
                     /locus_tag="AMK28_RS25920"
                     /old_locus_tag="AMK28_25540"
     CDS             complement(3132..3878)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459221.1"
                     /locus_tag="AMK28_RS25920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25540"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_073743676.1"
                     /transl_table=11
                     /translation="MADENFLHPRLAALYDPLDPDRGDLEPYVRLAEESGARRVLDIGC
                     GTGVFALLLADRGIEVVGVDPDRASLDVARAKPGGDRVRWIHGDAATLPPLRVDLVTLT
                     ANAAQEIVDPGDWEATLLGAHEALRPGGQLVFETRDPARRAWETWNRESSHRVTEIPGV
                     GPVETWVEVTEIVGPLVSFRWTYVFRRDGQVLTSDSTLRFRERHEVEADLSAQGFVVRE
                     VRGAPDRPGRELVLIARRPGPAPRRR"
     misc_feature    complement(4047..4059)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(4119..4988)
                     /locus_tag="AMK28_RS25925"
                     /old_locus_tag="AMK28_25545"
     CDS             complement(4119..4988)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382293.1"
                     /locus_tag="AMK28_RS25925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25545"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_073743678.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARRPERLTVRGDGVEVV
                     EGEYGDRAALDRALRGVASVFLVTNSPTEPDDERVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGIPWTFVRPRTFMSNTLSWAPGIRSTGVVRALYGDAPVAC
                     VDPRDVAAVAVAVLTGTGHEGRAYAVSGPEAITAREQTAQLSRVLERPLRFEELGVDAA
                     RTALLAKYPPPVAEAFLQSAERQRAGAKAAVVPTIQDLTGRPARPYRIWSAEHAEAFAP
                     EAGRFAGE"
     gene            complement(5001..5945)
                     /locus_tag="AMK28_RS25930"
                     /old_locus_tag="AMK28_25550"
     CDS             complement(5001..5945)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="AMK28_RS25930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25550"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_073743680.1"
                     /sec_met_domain="AfsA (E-value: 4.5e-27, bitscore: 89.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDPVPTMDAEAEVVHPVEIDMVHRTRPEDAFPRNWVRL
                     GHDRFSVGAVLPHDHPFFAPVGDDRHDPLLVAEAMRQAAMLAFHAGYGIPLGYQFLMSG
                     LDYVCHQEHLTVGDAPTEIDVEVFCSDLKWRGGLPAQGRVGWAVHRDGRLAATGMGETR
                     FSTPKAYRRMRGEAPAEGISIPETAPVSPVLAGRARAEDVVLSETGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAAWLAVGPAGIVPAEARTRFHRYSEFGSPCWIGAVVL
                     PETAEDTVTVQVTGHQDGEAVFSTILSGPRADG"
     gene            complement(6414..7025)
                     /locus_tag="AMK28_RS25935"
                     /old_locus_tag="AMK28_25555"
     CDS             complement(6414..7025)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="AMK28_RS25935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25555"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_073743682.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLIALGVAGVGALVVAWPSALTVVGLLGGVFLIGYGLLAARRVLRPSALHAGD
                     APAASRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASVCWFAA
                     LGFGARLLSGVLARPSAWRVLDGLVAVMMIGLGAKLALTA"
     misc_feature    complement(7048..7062)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.95"
                     /tool="antismash"
     misc_feature    complement(7083..7097)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            7093..8013
                     /locus_tag="AMK28_RS25940"
                     /old_locus_tag="AMK28_25560"
     CDS             7093..8013
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459228.1"
                     /locus_tag="AMK28_RS25940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25560"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_073743684.1"
                     /transl_table=11
                     /translation="MMFELPLDQVRTLLAVVDEGTFDSAATALRLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLEHDAQTALGMSGPGEPTILPIAVNSDS
                     LATWFLPALRRVPEELGLCYELRREDQDHTAALLREGLAMAAVTSSPEAVTGCSVRRLG
                     RMRYLPVASPGFADRWLGRREGTALRELIADAPVIGFDRRDDLQDAFVRRLGPGPRAST
                     RRHLVPTSEGFANAVAAGMGWGMVPEVQAEPLLRSGRLVRLTPDHTVDVPLYWQQWKLE
                     SPALAALSEAVAAEAAEALDGDRPG"
     misc_feature    8081..8099
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 25.73"
                     /tool="antismash"
     misc_feature    complement(8086..8104)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.95"
                     /tool="antismash"
     gene            8121..9191
                     /locus_tag="AMK28_RS25945"
                     /old_locus_tag="AMK28_25565"
     CDS             8121..9191
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459230.1"
                     /locus_tag="AMK28_RS25945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25565"
                     /product="methyltransferase"
                     /protein_id="WP_073743686.1"
                     /transl_table=11
                     /translation="MTDRHDQDAGNGREQRVDWEARGAVARLVLGQMATQALGTAVRLG
                     VFDRIGPDELSAEALAGSLGTHPQATLRLLRALAGLQLLSETEPGAFRTTTAGDALRAD
                     TPGTMVAMARMFTDPVMLRGWDLLDESVRTGETTFDTVFGTDFFGHLKEHPELSASFNE
                     AMSQGTRLTAATVPHHYDFGRFHTLVDIGGGDGTLLASVLREHPSLRGVLFDTAEGLAQ
                     APRRLAQEGLTERVALETGDFFASAPVGGDLYLLKSIIHDWDDERCAAILRHIRDVIPD
                     HGSLLIVEPVLPATVPPDRQDNVYLSDLNMLVNVGGRERTADDFAALCTAGGFVLRSVT
                     PLPAPNIFQIVEASPV"
     gene            9275..9949
                     /locus_tag="AMK28_RS25950"
                     /old_locus_tag="AMK28_25570"
     CDS             9275..9949
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="AMK28_RS25950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_25570"
                     /product="response regulator transcription factor"
                     /protein_id="WP_050361657.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLRDVIASGLRDGGFAVDCASDWPEADVLLHVTAY
                     DCVVLDRMVPSGDTLAPLEGRRRAGWSVPVLCLTALDSLHERLRGLESGADDYLAKPFS
                     MRELVLRVRGLSRRAPARLPSFLSCADVVMDVARHEVRRGGVLLSLSPKEYAVLQQLLV
                     HRDAVVTRTGLLEHCWDEMADPVSNVVDAVVAGLRRKLGSPGLVHTVRGQGFLLSAEPG
                     PS"
     misc_feature    complement(10826..10841)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.23"
                     /tool="antismash"
ORIGIN
        1 gcccggcacg gccccccgcg ccgccaccac gagggcgtcg gccagccggt cccggtagga
       61 gggcgcggcc ggatcgggca gcaggacttc gcaggcggtg aggagcagcg cgcccgccgt
      121 caggcccccg agccgctcga cgagcgtgcc gggggcgtac ggcggaaagc aggcgaggat
      181 gtagaacagc tggaggccgg gggcgatgcc cgcggggcgg gggccggcga cggcggcgaa
      241 cgccagcagg aatccgacga ggagcatgcc gccgacggcg gccggggtgt cggccgccag
      301 cagggttccc agggtcgcga ggaccagtgc cgggggcagg gcccgcagca tggtggaagc
      361 ccgttggcgg ccggagccgg gcacggtgga gagcaggccc atggcgatgg gcgcgaagag
      421 cgcgtacagc gccgcgacgg ggagtccggc cccgtacagc agcggataga acccggcgct
      481 cgccgcgccg gtgacgcgga cggcccggcg caggatcgtc gcccggtcct gcgcggcggt
      541 cacttccccg ctccgctcat ccggcgctcc tctcccgggc gggccgcggt ggcgtacctc
      601 cgtccggctc ccgccattgt cggacgccta cgagccgccc gcgcgcgctg gaccggagag
      661 cgcggcccgg agagcggcgc gggccgccag gacctcggcc tgcgcggcgt cgaggatcag
      721 gcccgcgacg gggtggggag gccggtggtt cgccgggcct gtccgaccca tgtccaccgc
      781 agcccgcggt gccgcgtccg ggcgacggcc cacccctccg ctccgggcgg agatccctcc
      841 ggggaccgcc ctgcgcgcct cggccgaccc catcgggccg cgcagcgccc aggagccggc
      901 ggacgggcac gtcgacatgg ccgggaaggg gcgctcccgg tcggcgggaa ccccggagca
      961 tgggcgcggg tccgccccga gcacctcatc gggcgctcgg ggcggacgct cgcgtggtgg
     1021 gggcggccgg gcggccgacg cctcagatcg tcgccgtgtc gatcacgaag cggtagcgga
     1081 cgtcgctggc gagaatccgc tcgtacgcct cgttgacctg gcccgcgtcg atcacctcga
     1141 tctcggcgcc gagtccgtgc acggcgcaga agtcgagcat ctcctgggtc tccttgatcc
     1201 cgccgatcgc cgaacccgcg atcgacttgt tgccgaggat cagggagaag aggttcagcg
     1261 agaccggctc ctcgggggcg cccacgttga ccagcgtgcc gtcggtccgc aggagaccga
     1321 ggtaggcgcc gaagtccagc ggggccgaga ccgtggagag gatgacgtcg aaggtgccgg
     1381 ccagttcctc gaaggtccgg gggtcgctgg tcgcgtggta gtggtcggcg cccagcttga
     1441 gcccgtcgtc cttcttgcgc agcgactggg agaggaccgt gacctcggcg ccgagcgcgt
     1501 gcgcgatctt gacggccatg tggccgagac cgccgaggcc gacgacggcg accttcttgc
     1561 cggggcccgc accccagcgc ttgagcgggg agtacgtggt gatgcccgcg cacaggagcg
     1621 gcgctgcctc gtccagggcg atgccctcgg gaatggagag ggcgaacgtc tcggccacga
     1681 cgatgtgcgt cgagtagccg ccgtgggtgg gctcgccgct gcggtcgaga gcgttgtacg
     1741 tctgggtgtt cccctcggcg cagtgctgct cgcggcccat caggcacgcg tcgcacgatc
     1801 cgcaggagtc gaccatgcag ccgacgccga cgcggtcgcc gaccttgaac cgggtgaccc
     1861 cggatccggt ctcggcgacg atcccggcaa tctcgtggcc gggcaccatc gggaagatgc
     1921 cctcgcccca cccgtcgcgg gcctggtgga tgtcggagtg gcagatcccg gcgaacttga
     1981 tgtcgatcag gatgtcgaac tcgcccaccg ggcggcgttc gatcgtggtg cgctccagcg
     2041 gggccttggc acggggagcg gcataggcgg caacggtggt catgccggag gttctcctct
     2101 gggtgatgcg gtaggaacgt ctcccagcat cacgccgcga cggatgttta cccaggtcac
     2161 cgctttgcct acgtccagcg gtcctactac tgacggggac aggctccggc gcgtacgacc
     2221 aggaatcaca ggaataatgg aagccatgga caatcagccg gagcccgccc ccgagccgct
     2281 ggaccggcgt gccgagctca gcgagttcct gcgcacccgg agggccaagc tccggccggg
     2341 cgacgtgggg cttccggagt tcggccggca ccgccgggtg cccggtctgc gccgggagga
     2401 gctggcacag ctggccgggg tgtccgtggc gtactacacg cgcctggagc agggcaacgg
     2461 gcgcaacgtg tcggccgagg tcctcgacgc gatcgcgcgg gccctgcggc tcacggacgc
     2521 cgagagcgcg catctgacac atctggccaa gcccgcgcgg cacaagaaga agcgccgccc
     2581 ggcccgggtg cagcgggtgc ggaaggggct gctgtatctg ctcgacagca tggacggcat
     2641 ccccgcgtac gtgaccgggg cgcgctccga cgtgctcgcc tggaatgcga tggcggcggc
     2701 ggtgttcggc gactggggcg cgctgccgcc tgccgagcgc aactgggcgc ggctggtgtt
     2761 cctctccccc gcctaccggg acctgttcgt gaactgggac tccaaggcct cggacatggt
     2821 cagctatctg cggctgtacg ccggctgcca ccccgacgat ccggaactgt cggcgctggt
     2881 gggcgaactc tccgtcaaga gcgatgagtt ccggcggctg tgggccacgc acaacgtcaa
     2941 ggagaagggg cacggcgtca agctgctgcg gcatccgctc gtcggggagc tgaccctgtc
     3001 gtacgagacg ctgaacctcc cggacgacga ggagcagcat ctggtgacct accacgcgga
     3061 gccgggctcg gagtcggccc agtccttgcg cctgctggcg agctggggtg ccgacgcggg
     3121 attgacggac gtcatcggcg acgcggggcg gggcccggcc gacgagcgat gaggacgagt
     3181 tcccggccgg gccggtcggg tgcgccgcgc acctcgcgta cgacgaaccc ctgtgccgac
     3241 aggtccgcct cgacctcatg acgttcgcgg aagcggaggg tggagtcgga ggtgaggacc
     3301 tgcccgtcgc gccggaacac gtaggtccag cggaacgaca cgagcggccc tacgatctcg
     3361 gtcacctcga cccaggtctc gacggggccg acgccgggga tctcggtgac gcggtgggaa
     3421 ctctcccgat tccatgtctc ccaggcgcgc cgggccggat cccgggtctc gaagaccagt
     3481 tgcccgccgg gccgcagcgc ttcgtgggcg ccgagcagtg tcgcctccca gtcgcccggg
     3541 tcgacgatct cctgcgcggc gttggccgtc agggtgacca ggtcgacccg caacggggga
     3601 agggtcgcgg cgtcaccgtg gatccaacgg acccggtcgc cgcccggctt ggcgcgcgcc
     3661 acgtcgagcg aggcccgatc ggggtcgacc ccgacgacct cgatcccacg gtccgccagc
     3721 agcagggcga acacgcccgt accgcagccg atgtccagca cccgccgcgc gccggactcc
     3781 tcggcgaggc ggacgtacgg ttccaggtcg ccccggtcgg gatcgagtgg gtcgtagagg
     3841 gcagccagcc ggggatgcag gaaattctcg tcagccatgg ggcggaaggt acagggtcct
     3901 gtcgctggag caacgtgcgg aactggaccc gcgtacccgg gagatcggga gattgtccga
     3961 ccgactcacc ggtttccctt tcggagaaac ggaggaatgc ggctgaggcc cccgagcctt
     4021 tttcgggctc acctgtcctg aaaaccgtga aagcggcacg caaaaaggaa tactagcgat
     4081 gtcgcacata tccgcggccc gcaagaagcc gatgcgtcct actcgcccgc gaaacgaccg
     4141 gcttccggcg cgaacgcctc cgcgtgctcg gccgaccaga tgcggtacgg cctggccgga
     4201 cggcccgtca ggtcctggat cgtgggcacc acggccgcct tcgcgcctgc ccgctgccgc
     4261 tccgcgctct gcaggaaggc ctcagcgacg ggcggcggat acttcgccag cagggccgtg
     4321 cgcgcggcgt ccacgcccag ttcctcgaag cgcaacggcc gctccagcac ccgggagagc
     4381 tgagccgtct gctcccgcgc ggtgatcgcc tccgggcccg acacggcgta cgccctgccc
     4441 tcgtgtcccg taccggtgag aacggcgacg gccaccgcgg caacgtcacg tgggtcgacg
     4501 cacgcgaccg gggcgtcgcc gtacagggcg cgcaccactc ccgtcgaccg gatgccgggc
     4561 gcccaggaca gcgtgttgga catgaaggtc ctggggcgga cgaacgtcca cgggatcccc
     4621 gactccctga ccgcctgttc gttctcccgc tgacgccggg tgatgaagtc gtcggcgccc
     4681 ggctcctcca ccgccatcat ggacagcttg accaggtggc gtacgcctgc cgcggccgcc
     4741 gccgcggcga cccgctcgtc gtcgggttcc gtggggctgt tcgtcaccag gaacaccgag
     4801 gcgacacccc gcagggcccg gtcgagagcg gcgcggtccc cgtactcccc ctcgacgacc
     4861 tcgaccccgt ctccccgtac cgtgagccgc tcgggacggc gcgcgagaat ccgcacgggg
     4921 ccggccgccg ccagttgtcc ggcgacctca cggcccaccg ctccggtcgc gccagtcacg
     4981 agaatcactc gaggaacctc tcagccgtcg gcccgggggc cggacaggat ggtggagaac
     5041 accgcttcac cgtcctgatg gcctgtcacc tggaccgtca ccgtatcctc ggccgtctcc
     5101 ggcaggacca ccgcccctat ccagcacgga ctgccgaact cggagtaccg gtggaaccgg
     5161 gtgcgcgcct ccgccggcac gattccggcg ggccccaccg ccagccacgc cgcctggcgg
     5221 gccgcctcca gcagcaacat gcccgggacg tggtcgttgg gtcgctggaa cagggtggga
     5281 tgccgggtgt ccacacgcag ctcccagact ccgtcccgcc cggtctccga gagcaccacg
     5341 tcctcggcgc gggcacggcc cgccaggacg ggcgaaaccg gcgcggtctc cggtatggat
     5401 atcccctcgg ccggggcctc gccgcgcatc cgcctgtacg ccttgggggt gctgaaccgg
     5461 gtctccccca tccccgtcgc ggcgagccgg ccgtcccggt gcaccgccca gccgacacgc
     5521 ccctgcgccg ggagcccgcc ccgccacttc aggtcggagc agaacacctc cacgtcgatc
     5581 tcggtggggg cgtcccccac ggtcaggtgc tcctgatggc agacgtagtc cagtcccgac
     5641 atcaggaact ggtagccgag cggtatcccg taaccggcgt ggaaggcgag catggccgcc
     5701 tgccgcatcg cctccgcgac cagcagcggg tcgtgccggt cgtcacccac cggggcgaag
     5761 aacggatggt cgtgcggaag aaccgccccc accgagaacc ggtcgtgccc caggcgtacc
     5821 cagttccgcg gaaaggcgtc ctccggcctg gtccggtgca ccatgtcgat ctccacggga
     5881 tgcaccacct cggcctccgc gtccatggtc ggcaccggat cgatcaagac tgctgcttcg
     5941 ggcataactc cccccagggg tcatgtagtg gcgagccgtt cctgcgatgc ccataccggc
     6001 aagattaggt acagactaat aggttttctt ctccggccaa ggtgagaccc cccacaccgg
     6061 cccggccgat tcgcgggccc cgcccgggaa tccggatggc ggcggcgcgg gcatccggta
     6121 aatgcgtccg ccaatccacg agaatcaccg gaattcccga aagcgccaac tgtacgaaag
     6181 gcgatatccg ctgccgtttc acgtgcggcc acggtctccc ggcccgggag accgggctcg
     6241 aacggatgcg acccgtccag ggcgagaacg acggcccgtt gcacgcttcc catggcacga
     6301 tcctgtcgga agatggcgat catggcatgg tacgccgggc gggcaccctc gaccctggac
     6361 gcccgaagcg cccagggagg tccctgggcg caagcggtca ggcggtcagg cggtcaggcg
     6421 gtcagcgcca gtttcgcgcc gaggccgatc atcatcacgg cgaccaggcc gtccagcacc
     6481 cgccacgcgg aaggccgcgc gaggactccg ctcagcaacc gggcgccgaa cccgagcgcg
     6541 gcgaaccagc acacgctggc gagaacggcg ccgaggccga aggtccagcg caacggcccc
     6601 cggtcggcgg cgacggaccc gagcaggaac acggtgtcga ggtacacgtg cgggttgagc
     6661 caggtcatcg ccaggcaggt cagcaccacg ccccggcgtg aggcggccgg ggcgtccccc
     6721 gcgtgcaggg ccgacgggcg caggacccgg cgcgcggcga gcaggccgta gccgatcagg
     6781 aacacgccgc cgagcagccc caccaccgtc agcgccgacg gccaggcgac gacgagagcg
     6841 ccgaccccgg cgacccccag cgcgatgagc accgcgtcgg acagggcgca gatcccgacc
     6901 accgcgagca cgccgtcacg gcggacgccc tggcgcagca ccagggcgtt ctgggctccg
     6961 atggcaacga tgagcgagag gccggtgccg aaaccggcgg ccgcggtggt cagggcgttc
     7021 gtcatgcggt cgacgctacg gagcggagga acatggtgtc cagctaaaga ttcttacgta
     7081 tcattaacag tcatgatgtt cgagcttcct ctcgaccagg tgcggaccct gctggccgtg
     7141 gtcgacgaag gcacgttcga ctcggcggcc accgctctgc ggctgacgcc gtcggcggtc
     7201 agccagcggg tcaaggcact ggagcagcgc acgggccggg tgctgctgat gcgcacgaag
     7261 ccggtgcggc ccaccgagtc cggtgaggtg gtggtgcgct tcgcccgcca gctggccagg
     7321 ctggagcacg acgcgcagac ggcgctcgga atgtccggtc ccggcgagcc gaccatcctg
     7381 ccgatcgcgg tgaactccga ctccctggcg acctggttcc tgcccgccct gcggcgcgtg
     7441 ccggaggagt tggggctctg ttacgagctg cgccgggagg accaggacca tacggccgcc
     7501 ttgctgcggg agggactggc gatggccgcg gtgacctcct cgccggaggc ggtgacgggc
     7561 tgctcggtgc gccggctggg ccggatgcgg tatttgccgg tggccagtcc cggtttcgcc
     7621 gaccggtggc tgggacgccg ggagggcacc gcgctgcgcg agctgatcgc cgacgccccg
     7681 gtgatcggct tcgaccggcg cgacgacctt caggacgcct tcgtgcgccg gctcggcccc
     7741 ggcccccggg cgagcacgcg gcgccacctg gttcccacgt cggaggggtt cgcgaacgcc
     7801 gtggcggcgg gcatgggctg gggcatggtg cccgaggtcc aggcggagcc gctgctgcgc
     7861 agcggccggc tggtgcggct caccccggac cacaccgtgg acgtgccgct gtactggcag
     7921 cagtggaagc tggaatcacc ggccctggcg gccctctccg aggcggtggc ggcggaggcc
     7981 gccgaagcgc tcgacggcga ccggccgggc tgagcggcac accgcccgcc gaccggaacc
     8041 ccgcccgcca cgcctatggg tgattgctac cccacccctc caaggttagg tttacctaac
     8101 tgaacggaaa ggggcaggtg atgacggacc gtcacgacca ggatgcgggc aacggccggg
     8161 aacagcgggt ggattgggag gcacgggggg cggtggcccg gctcgtgctc ggccagatgg
     8221 ccacccaggc gctgggcacg gcggtgcggt tgggcgtctt cgaccggatc gggccggacg
     8281 agctgagcgc cgaagcactc gccggttccc tcggcaccca cccgcaggcg acgctgcgtc
     8341 tgctgcgcgc cctggccgga ctccagctgc tgtccgagac ggaaccgggc gccttccgga
     8401 ccaccacggc cggcgacgcc ctgcgcgccg acacacccgg cacgatggtc gccatggccc
     8461 ggatgttcac cgacccggtc atgctgcggg gctgggacct gctcgacgag agcgtccgta
     8521 cgggagagac cacgttcgac acggtgttcg ggaccgactt cttcggccat ctcaaggaac
     8581 accccgagct gtccgcctcg ttcaacgagg cgatgagcca gggcacacgg ctgaccgccg
     8641 cgaccgtccc gcaccactac gacttcgggc gcttccacac gctcgtggac atcggcggcg
     8701 gcgacggcac tctgctggcc tcggtccttc gcgagcaccc gagcctgcgg ggcgtcctgt
     8761 tcgacacggc cgaggggctc gcgcaggctc ctcgacggct ggcccaggag ggtctgaccg
     8821 agcgcgtcgc cctggagacc ggcgacttct tcgcctcggc cccggtcggc ggcgacctct
     8881 atctgctcaa gagcatcatc cacgactggg acgacgagag gtgcgcggcc atcctgcggc
     8941 acatccgcga cgtcatcccc gaccacggct ccctgctgat cgtcgaaccg gtgctgcccg
     9001 ccaccgtgcc gccggaccgg caggacaacg tctacctcag cgacctcaac atgctcgtca
     9061 acgtcggcgg ccgggagcgg accgcggacg acttcgcggc cctgtgcacg gcgggcggct
     9121 tcgtcctccg gagcgtgaca ccactgcccg cgcccaacat cttccagatc gtcgaggcct
     9181 cgccggtctg acgggccccc cgggggcggg tgcccggccc gccttagaat gacgggtccc
     9241 tccccccgga agaagactcg gaggcatcct ccccatgcgt gtgctgctgg tggaggacga
     9301 cgacgacctg cgggacgtga tcgcgtccgg gctgcgggac ggcggcttcg ccgtggactg
     9361 cgcgtcggac tggccggagg cggacgtgct gctgcacgtc accgcgtacg actgtgtggt
     9421 gctggaccgg atggtgccct ccggcgacac cctcgcgccg ctggagggca ggcgtcgggc
     9481 cggctggtcg gtgcccgtgc tgtgcctcac cgctctggac tccctgcacg aacggctgcg
     9541 ggggctggag agcggggcgg acgactacct ggccaagccg ttctcgatgc gcgagctcgt
     9601 cctgcgcgtc cggggcctga gccggcgggc accggcccgg ctgccttcct tcctgagctg
     9661 tgcggacgtg gtcatggacg tggcgcggca cgaggtgcgg cgcggtggtg tgctgctgtc
     9721 cctgagcccg aaggagtacg ccgtgctcca gcagctcctc gtccaccggg acgccgtcgt
     9781 cacccgcacc gggctgctgg agcactgctg ggacgagatg gccgacccgg tctccaatgt
     9841 ggtggacgcc gtcgtcgcgg gcctgcggcg caagctcggc tcccccggcc tggtgcacac
     9901 cgtgcgggga caggggtttc tgctgtcggc ggagccgggg ccgtcctgat ggcgaagccc
     9961 ctgcggcccc gccgctcccc cgccaggagg cggctgcacc ggctgcggct gcggctgacc
    10021 gccgcgtaca ccctgatcac cgtcgtcggg ctggcctgtc tgtcctggct ggtgatccgt
    10081 accgacgacc gggcgcggct ggacgccgag tacgacgaga tgcgccgccg ggcgtccgtc
    10141 gcggcctcgc tcgtctatta cgaggacggc cggatccgtc tggacgggct cgacgacgac
    10201 gaggcgacgt cggggactcc gcaggtcctg gtgctggagg gccggcccgg cggtgatccg
    10261 gtcatcgtgt tccggggccg caccgcgcag ttcgccgtgc cggccggggc gctccgggct
    10321 gcggctctct cggcgatgga cgacgagggg ccggtgcggg ccgaggcgcg cgaccgcgcg
    10381 ggagaggccg ttcacgtgct ggccgtgccc ttctaccacg acgccaccga cgaggtggcg
    10441 ggcgcggccg tcgcggtcgg agatccggag cgcggggcgg ccgagcaccg cagtctggtc
    10501 ctctccctcg tcgtcggggc cggtgcgctg acggcgctgg ccgcgctgac cgggcatgtc
    10561 ctctccgcac ggagcatgcg gccggcctgg gaggcgctgg agcagcagga gcgactgctg
    10621 gccgacgcgg cgcacgagtt gcggaccccg gtcgcggtga tgcgcggatc ggtggaggtg
    10681 gccgccggtg ctccgggcgg gctggaggct catctgcccc ggatccggcg ggcggcggac
    10741 cggatggccg acgtcgtgga gaacctgctg acgcgggggc ggttggaggc cgccgtggac
    10801 gtcgtacggg cggagccact gcggctggac cagctcgtcg aggaggtgtg cgcggagctg
    10861 ccggagggcg ggcacggcct ggagtcgcgg tgggaggagt cggtggtcga ggcggacgcc
    10921 gcgctcgtgc gggtcgccgt gcgca
//
