LOCUS       NZ_LIVW01000004        11329 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02115 scaffold4, whole genome shotgun sequence.
ACCESSION   NZ_LIVW01000004
VERSION     NZ_LIVW01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02115
  ORGANISM  Streptomyces sp. CB02115
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 759400)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759400)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905645.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,651
            CDSs (total)                      :: 7,572
            Genes (coding)                    :: 7,475
            CDSs (with protein)               :: 7,475
            Genes (RNA)                       :: 79
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 79 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 424161
            Orig. end                         :: 435490
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVW01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6329)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    275..290
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    complement(826..842)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            1277..2005
                     /gene="hemQ"
                     /locus_tag="AMK28_RS23525"
                     /old_locus_tag="AMK28_23215"
     CDS             1277..2005
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="AMK28_RS23525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23215"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_026290345.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDITVRGTYDVSGLRADADLMIWWHAETSDELQEAYNLFRRTKL
                     GRALDPVWSNMALHRPAEFNKSHVPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWVLAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     gene            complement(2165..3727)
                     /locus_tag="AMK28_RS23530"
                     /old_locus_tag="AMK28_23220"
     CDS             complement(2165..3727)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     6.9e-19. Score: 60.3. Matches aSDomain:
                     nrpspksdomains_AMK28_RS23530_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="AMK28_RS23530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23220"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_073743171.1"
                     /sec_met_domain="PF00561 (E-value: 3e-25, bitscore: 84.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLSALAAAPAGAWEGRGSAEARGTAIAAARAT
                     AAGIDFVSCPEKEMLPDSLKCGTVKVPLDYAEPDGEQLELTVSRTGATGPADARQGAFV
                     YNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLTCQDPAAYTKGPT
                     DAPTHPTQEYKERRVARAKAYAEGCATHAGETLRHYTSLNNARDLDVLRAALGEPRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSVVDPDPEQIWYRSNMGQSLAFEKRWEDFRRW
                     VAKHDDVYHLGDTPESVQRHYDEVRAELAVEPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAAEDNENAQAVYTAVECNDAPWPEDFEVWDRDHTD
                     LARVAPFETWDNAFANLPCAFWPAPRQRPLEVGTRWGELPPVLILAAERDAATPYKGAL
                     ELRERLPGSSLVTERDAGTHGIGGAGNTCVDDHLRRYLLTGEVPGRGADCAAHPEPNPV
                     SLD"
     aSDomain        complement(2300..3424)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK28_RS23530_Abhydrolase_1.1"
                     /evalue="6.90E-19"
                     /label="AMK28_RS23530_Abhydrolase_1.1"
                     /locus_tag="AMK28_RS23530"
                     /protein_end="476"
                     /protein_start="101"
                     /score="60.3"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SAPLTCQDPAAYTKGPTDAPTHPTQEYKERRVARAKAYAEGCATHAGETLRHYTSLNNA
                     RDLDVLRAALGEPRLTFMGASYGTYFGALYATLFPSHVRRMVFDSVVDPDPEQIWYRSN
                     MGQSLAFEKRWEDFRRWVAKHDDVYHLGDTPESVQRHYDEVRAELAVEPAGGKVGPGQL
                     HSAFLGAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAAEDNENAQAVYTAVECN
                     DAPWPEDFEVWDRDHTDLARVAPFETWDNAFANLPCAFWPAPRQRPLEVGTRWGELPPV
                     LILAAERDAATPYKGALELRERLPGSSLVTERDAG"
     gene            complement(3877..4995)
                     /locus_tag="AMK28_RS23535"
                     /old_locus_tag="AMK28_23225"
     CDS             complement(3877..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072528.1"
                     /locus_tag="AMK28_RS23535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23225"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073743409.1"
                     /transl_table=11
                     /translation="MNVLALLATLAVVVSSVLLIVKSVAERSRRPSPGPGVALHDLYEV
                     AFLNGGPARVVDTALTALHADGRLAVGGPGIVAAQRVQANDPVERAVFQELAAAPNGAL
                     HVLREAVMRHPAVQEVGDGLAARGLLVPPHRLRPLRRWGMTQGIVCLIAVPLSMLLTFV
                     QFATDDSLDFSVPFIVKMLPAIVIGSFTGMIVASSSAGRLTASGRHAAQSFRTAYAYVV
                     APAHLVATLGLRALPDPVFQAQLMAAARLSAPRRRSSARVAGSTATGVTAVGAATVWCA
                     ASGPGGSSCGGSTGGGSGSSCGGGSGCSSGSGCGGSGSGSSCGGGSGCSSGSGCGGSGS
                     GSSCGGGSSCGSSSGSSCGGGGGGSSCGGGGS"
     misc_feature    complement(4997..5008)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            complement(5001..6329)
                     /locus_tag="AMK28_RS23540"
                     /old_locus_tag="AMK28_23230"
     CDS             complement(5001..6329)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452208.1"
                     /locus_tag="AMK28_RS23540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23230"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073743172.1"
                     /sec_met_domain="DUF692 (E-value: 8.9e-91, bitscore: 299.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVESLSGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPAPDRLAGLAARAELLGTPLVTEHIAFVRAGGSLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALITWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGLDPAKALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVTAPVLEVLAELRSRVDPPGVLLERDDAFPPAEGLAGELDAIRATLRKAAPPAGPRP
                     GPAPASDDSAGTGAGPRTAAPAPVPAGTRDRTAVAQTALLSALVAGTPAPEGFDHRRLR
                     VQSRALTAKRADVVAKVAPELPEILGDGYRAAFLAYAGNRPMSGGYRRDALGFAEHVLI
                     AGGPADPVARRRLTYWWQDRSGARPPRRTTRLVRAARAALVRK"
     gene            complement(6452..7198)
                     /locus_tag="AMK28_RS23545"
                     /old_locus_tag="AMK28_23235"
     CDS             complement(6452..7198)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="AMK28_RS23545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23235"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_198957957.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLGALAFPVWSYADRSGTGEAHLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHADLDRRAR
                     DVAAKLGVELPNQPTAQQQGWLRELTAASGDAYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAASGSTAGPSGPSMPQDGQAPQ
                     VPLPVTPTGDQSFTSRPVPPTMMPMP"
     gene            complement(7339..8067)
                     /locus_tag="AMK28_RS23550"
                     /old_locus_tag="AMK28_23240"
     CDS             complement(7339..8067)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="AMK28_RS23550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23240"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_073743174.1"
                     /transl_table=11
                     /translation="MTSDDTPAPVPESPFHDGPTDRDQAQQFVLPLVLHLEKTDPPART
                     DALRTAARAVLTILSDERSLGDGEWAVAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VTGEDGAEVRVFPPIPLDGWPRELAKLQVSGTDLEDPEPPAAPDPAGPVLWLNPDLDMS
                     AGKTMAQVGHGAQLAWWELGDTERKAWREAGFPLAVATPGAERWRELTVSGLPVVRDAG
                     FTEIAPGSCTVVADHPALRT"
     gene            8139..8813
                     /locus_tag="AMK28_RS23555"
                     /old_locus_tag="AMK28_23245"
     CDS             8139..8813
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="AMK28_RS23555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23245"
                     /product="AIM24 family protein"
                     /protein_id="WP_073743175.1"
                     /transl_table=11
                     /translation="MKSDLFSSDHMAQPATAPGMTLQNTKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGLLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDSIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLTTSLNRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            8831..9850
                     /locus_tag="AMK28_RS40190"
                     /old_locus_tag="AMK28_23250"
     CDS             8831..9850
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452203.1"
                     /locus_tag="AMK28_RS40190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23250"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_241837559.1"
                     /transl_table=11
                     /translation="MGHRYGLRGAWVLRGVDLTLPARTLVRVEGGNGAGKSTLLRLLAG
                     IDAPAEGRITGRRARTAYVPERFPAALPLTATGYLVHLGRIHGLRSAEAKRRAGEWLTR
                     FGAADHARTPLAELSKGTSQKVAVAQALLAEPDLLVLDEAWTGLDTAARAELDRAVAER
                     VAAGATVVFVDHDPRRLAGAADLTLRTGGGGVHRVTAPAPTGPASGPHVLIEAVGPPGA
                     PLPEDLPGDPARDRRTGAVPGLVRLTVPAPYSDAVLAALLTARPPWHIRQVAPAEAHGP
                     AEAPAHGAAEAPAHGAAEAPAYGPTPTGPAGSQVSAKAPAQAPVRARTPEAPEVSEAP"
     gene            9847..10515
                     /locus_tag="AMK28_RS40195"
                     /old_locus_tag="AMK28_23255"
     CDS             9847..10515
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="AMK28_RS40195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23255"
                     /product="ABC transporter"
                     /protein_id="WP_241837560.1"
                     /transl_table=11
                     /translation="MIALLRYQAALLARSQRWLAPLLLYAAVLAVGVRSGQPVLGSLGV
                     AAAAVLPVTAWAVHLCVAQEPPAARTVASAAAGPRRVHLAALLTATGCAAALAAVTGAV
                     VLFISSPVTDDGARAVSRPSAALAGLLAVLCCVLLGAAVGALSTRPVPLDRGWSVAAAV
                     LGSLLALVTTGSPARLAVTALVTGSRTGAVQPSVLPLLGAAALAAASAFFVCRIAARRG
                     "
     gene            10524..10745
                     /locus_tag="AMK28_RS23565"
                     /old_locus_tag="AMK28_23260"
     CDS             10524..10745
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452201.1"
                     /locus_tag="AMK28_RS23565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_23260"
                     /product="hypothetical protein"
                     /protein_id="WP_073743176.1"
                     /transl_table=11
                     /translation="MGVDTDTNTDTDDTYCETPGPIAPDPSGGAAEGPPYAECVLCREP
                     TEYPESTKGATLCPVCAWQEAGRTACSG"
ORIGIN
        1 cccgaggcgg tcgccctggc ccgcgaggtg ggcctcgccg accgcctcca gccgcccgcc
       61 accgccaccg cctcgctgtg gacgcgcgac gcgctgcgcc ccatgccgaa ggggcacgtc
      121 atgggcgtcc ccggcgaccc ggcggtgctc ggcgaggtgc tgtcccccga gggcctggcc
      181 cggatcgccg aggagcgcga cctgaccccg acccccgtcg gcgacgacgt cgcggtcggc
      241 gcgtacgtcg ccgaccggct cggccgcgag gtcgtggacc ggctggtgga gcccctcctc
      301 ggcggggtct acgccggtga cgcctaccgg atctcgatgc gcgccgccgt cccccagctc
      361 ttcgaggcgg tgaaggaggg cgggccgctg ctggagggcg tacgccgtat ccaggaacgg
      421 gccgccgccc ggcagcagac cggccccgtc ttccagggcg tcgagggcgg catcggcacc
      481 ctcccggccg ccgtcgccga cgccgtacgg gccggtggcg gcgagatcct cgaccggacc
      541 cccgtcctcg gcctgacccg tacggagtcg ggctgggcgg tccgcaccga cacccggacg
      601 atcgccgccg acggcatcgt cctggccacc cccgcctggt ccgccggcac tctgctggcc
      661 gccgagtccc cggccgcgtc cgccgagctg tccggcgtcg agtacgcctc gatggccctg
      721 gtcaccctgg ccttccggcg ggccgacatc gagcgccacg aggccctgcg cggccgttcc
      781 ggcttcctcg taccgccggt cgacgggcac acgatcaagg cgtccacgtt ctccagcaac
      841 aaatggcagt gggtcgcgga cagcgccccc gacctgttcg tgctccgcac ctccgtcggc
      901 cggtacggcg aggaggacca tctgcaccgc gaggacgagg agctcgtcgc cgtctccctg
      961 cgcgacctcg ccgcggccac cggactcacc gcccggcccg tggacaccga ggtcacccgc
     1021 tggatcggcg gcctgccgca gtacccggtc ggccacctcg cccgggtcgc ccggatccgc
     1081 gacgaggtcg ccaagctccc cgccctgcgg gtctgcggcg cggtctacga cggggtcggc
     1141 atccccgcct gcatcgcgag cgcgcgccgg gcggccgacg agatcgcggg ggagctcacg
     1201 ggggagatcg cgggagagat catcgccacg tcgaccctgg ttcggggcac ggggagcgag
     1261 gcgggacaat agccgtatga gtgctcctga gactgtgaca tcaagcaaga gtccgaacgc
     1321 cggcaagaag gccaaggacc tcaacgaggt catccgctac accctgtggt cggtcttcaa
     1381 actgcgcgac gtgctcccgc tggaccgcgc gggatacgcc gacgaggtcc aggagctgtt
     1441 cgaccagctc gcggccaagg acatcaccgt ccgaggcacc tacgacgtgt ccggtctgcg
     1501 ggccgacgcc gacctcatga tctggtggca cgccgagacc tcggacgagc tccaggaggc
     1561 gtacaacctc ttcaggcgca ccaagctcgg ccgcgccctc gacccggtct ggtcgaacat
     1621 ggcgctgcac cggccggccg agttcaacaa gtcccacgtc ccggcgttcc tcgccgacga
     1681 gacgccgcgc gactacatca gcgtctaccc cttcgtccgg tcctacgact ggtacctgct
     1741 gcccgacgag gaccgccgcc gtatgctcgc ggaccacggc aagatggccc ggggctaccc
     1801 ggacgtccgc gccaacaccg tcgcctcgtt ctcgctgggc gactacgagt gggttctcgc
     1861 cttcgaggcg gacgagctgt accgcatcgt cgacctgatg cgtcacctgc gcgcctccga
     1921 ggcgcgtctc cacgtgcgcg aggaggtccc gttctacacg ggccgcagga agagcgtcgc
     1981 agacctggtg gccgggctcg cataaccagc gagtcccgca ccaccccaac ccggaagatc
     2041 agaagggcgg gcaccgggca ccgatcgtgc cgtccgctcc tccagcgaca ccgggttcgg
     2101 cttcgggggc cgtcgcaccg tcatggtgcg gcggcccccg ctccgtaccg cccccgccgg
     2161 gggatcagtc cagcgacacc gggttcggct ccgggtgcgc ggcgcagtcc gcaccccgcc
     2221 ccggcacctc gccggtcagc agataccggc gcagatgatc gtcgacgcag gtgttcccgg
     2281 ccccgccgat gccatgcgtc ccggcgtccc gctcggtcac cagcgacgac ccgggcagcc
     2341 gctcccgcag ctccagcgcc cccttgtacg gggtcgccgc gtcccgctcc gccgccagga
     2401 tcagcaccgg cggcagctcg ccccaccgcg tcccgacctc cagcggccgc tgccggggcg
     2461 cgggccagaa ggcgcacggc aggttcgcga aggcgttgtc ccaggtctcg aacggtgcga
     2521 cgcgcgccag gtccgtgtgg tcgcggtccc acacctcgaa gtcctcgggc cagggcgcgt
     2581 cgttgcactc gacggccgtg tacaccgcct gcgcgttctc gttgtcctcg gccgccaccg
     2641 cacgcgggga cgcctgtgcg accagcggct ccggattgcc ccgcacgtac tccgacagcg
     2701 ccgtcgcccg catcgcccag tagtcgtcgt agtaaccggc ccccaggaac gccgagtgca
     2761 gctgccccgg cccgaccttc ccgcccgcgg gctccaccgc aagctcggcg cgcacctcgt
     2821 cgtagtgccg ctgcacggac tcgggtgtgt cccccaggtg gtagacgtcg tcgtgcttcg
     2881 ccacccagcg gcggaagtcc tcccaccgct tctcgaaggc cagcgactgg cccatgttcg
     2941 agcggtacca gatctgctcg gggtcggggt cgaccaccga gtcgaagacc atgcgccgta
     3001 cgtgggaggg gaacagcgtc gcgtacagcg cgccgaagta cgtcccgtag gacgccccca
     3061 tgaacgtcag acgcggctcg ccgagagcgg cccgcaggac gtccaggtca cgggcgttgt
     3121 tcagcgacgt gtagtgccgc agtgtctcgc ccgcgtgcgt cgcgcagccc tccgcgtacg
     3181 ccttcgcacg ggcgacgcgc cgctccttgt actcctgcgt cgggtgggtt ggcgcgtcgg
     3241 tcgggccctt cgtgtacgcc gccgggtcct ggcaggtcag cggcgcggag gagccgttca
     3301 ccccgcgcgg ggcgtagccg accaggtcgt acgcctcggc gatctccttc cactccggca
     3361 gctccccggc catcgggaag gtgatgctgg acgcgccggg gccgcccggg ttgtagacga
     3421 acgcgccctg ccgggcgtcg gccgggccgg tggccccggt gcggctgacg gtcagttcga
     3481 gctgttcccc gtccggttcg gcgtagtcga ggggcacctt caccgtgccg cacttcaggg
     3541 agtccgggag catctccttc tccgggcagg agacgaaatc gatccccgcg gccgtggcgc
     3601 gggcggcggc gatcgcggtg ccccgggcct cggcggagcc ccggccctcc caggctcccg
     3661 cgggagcggc ggcgagggcg gacaggacca gggagccgat cgatgtgtag agcgcagcag
     3721 ttctcacgcg cggtggcctc tctgcggatg cggagaggaa gagtcgggcg gccggacgga
     3781 tatgtcgagc accgccccgg agggtcgccg gaatccaccc tgatggacgc tcaagaaccg
     3841 tacgtacgaa cctgattcag gtcgcgggag accgattcag gagcccccgc ccccacagct
     3901 ggatccgccg cccccgcccc cacagctgga gccgctggag ctgccgcaac tggagccgcc
     3961 tccgcagctc gacccgctgc cgctgccgcc gcacccggag cccgaggagc acccggagcc
     4021 gcctccgcag ctcgacccgc tgccgctgcc gccgcacccg gagcccgagg agcacccgga
     4081 gccgcctccg cagctcgatc cggagccgcc cccggtcgac ccgccgcaac tggagccgcc
     4141 gggaccggac gcggcgcacc agaccgtcgc cgcgccgacg gcggtgacgc ccgtcgccgt
     4201 ggacccggcg acgcgcgcgg aggaccgtcg gcgcggagcg ctcagccgtg ccgcggccat
     4261 caactgggcc tggaacaccg ggtccggcag ggcgcgcagg cccagggtgg cgacgagatg
     4321 ggcgggggcc accacgtagg cgtaggcggt ccggaacgac tgggcggcgt ggcgccccga
     4381 cgccgtgagg cggcctgcgg aggagctggc gacgatcatc ccggtgaacg agccgatcac
     4441 gatcgcgggc agcatcttca cgatgaacgg gaccgagaag tccagggagt cgtccgtggc
     4501 gaactggacg aacgtcagca acatgctcag cggcacggcg atcaggcaca cgatcccctg
     4561 ggtcatcccc caccgccgca gcgggcggag ccggtggggc ggcaccagca gcccgcgagc
     4621 cgcgagcccg tcgccgacct cctggaccgc cgggtgccgc atcaccgctt cccgcaggac
     4681 gtgcagggcg ccgttcgggg cggcggccag ctcctggaac accgcgcgtt ccaccgggtc
     4741 gttcgcctgt acccgctggg cggccacgat gccgggcccg ccgacggcca gccggccgtc
     4801 cgcgtgcagg gcggtcaggg cggtgtccac gaccctggcc ggaccgccgt tcaggaacgc
     4861 gacctcgtac aggtcgtgaa gggcgacgcc ggggcccgga gaggggcggc gggaccgctc
     4921 ggccaccgac ttgacgatga gcaggaccga tgagacgacg accgcgaggg tcgccagcag
     4981 cgcgagcacg ttcacggcgt tcacttcctc acgagggcgg cacgggccgc ccggaccagg
     5041 cgggtggtac gacgcggcgg ccgggcgccc gaccggtcct gccaccagta cgtcagccgg
     5101 cgccgcgcca ccggatcggc gggaccgccc gcgatgagca cgtgctccgc gaagccgagg
     5161 gcgtcgcgcc ggtacccgcc ggacatcggg cggttcccgg cgtacgcgag gaacgcggcg
     5221 cggtagccgt cgccgaggat ctccggcagt tcgggggcca ccttggcgac gacgtcggca
     5281 cgcttggcgg tcagcgcccg gctctgcacc cgcagccgcc ggtggtcgaa gccctcgggg
     5341 gcgggcgttc cggcgaccag ggcggagagc agggcggtct gcgcgacggc ggtacggtcg
     5401 cgggttccgg ccggcacggg ggcgggggcg gccgtacggg gcccggcgcc ggtgcctgcg
     5461 ctgtcgtcgg acgcgggagc cgggcccgga cgcgggcctg ccgggggtgc tgccttccgc
     5521 agcgtcgccc ggatcgcgtc cagttcgccc gcgagcccct cggccggcgg gaaggcgtcg
     5581 tcgcgctcca gcagcacgcc cggcgggtcg acccgggagc gcagctcggc cagcacctcc
     5641 agcaccggag cggtcaccgg gtgggcgtgc gtgtcgtgcc agacgccgtt cttctcgacc
     5701 ccgcccgcca catggacgta cgcgatggcc tccaccggca gctcgtccag tgccttcgcc
     5761 gggtccaggc cgaggttgac gtgattggtg tgcaggttgg cgacgtcgat cagcagccgt
     5821 accccggtcc gctcgaccag ctccgccagg aactgcccct cggtcagctc ctcgtccggc
     5881 caggtgatca gcgccgcgat gttctccagg gcgagcggca ccggcagcga gtcctgcgcg
     5941 atccgcacgt tctcgcagag cacgtccagc gcgtcccagg tgcgcggcac cggcagcaga
     6001 tgccccgcct ccagcctcgg cgacgcggtg agcgaccccc cggcccgtac gaacgcgatg
     6061 tgctcggtca ccagcggggt ccccagcagc tcggcgcgcg ccgcgagacc ggcgagccgg
     6121 tcgggggcgg gccggtccgc gccgccgagg ccgagcgcca ccccgtgcgg cacgacggtc
     6181 accccgcgct cccggagccg caccagcgag tccgggaggt ggtcggcgca gaggttctcc
     6241 gcgaccgcct ccacccagtc gatcccgctc agcgactcca cctcggccgc gatctccggc
     6301 cgccagccga taccgatacc cagttccatg acgccccccg tccccctgct cacctcgttc
     6361 tgtccagggg tcatggcccc gtcacgcggc gatgaacccg accaggggga cgttcagagc
     6421 tggatttgag gttcccgtac cgccggtccc gttacggcat cggcatcatc gtgggcggca
     6481 ccgggcggga ggtgaaggac tggtcaccgg tcggcgtcac cggcagcggg acctgcggcg
     6541 cctgtccgtc ctggggcatc gacgggcccg aggggccggc cgtcgacccg ctcgccgcct
     6601 cgcgcgccag ggcgtcgaag tcgacgaagc cggtggcctc cagcatggtg atgtggtcca
     6661 gcacggtctg gttggcgtcg ctcgcgagct gccggatcag cgtgttccgg gtggtgtggc
     6721 ggacctggcc gatcaggccg aagaccttgc cgtgggcggc ccgcaggata ttggcgaact
     6781 tccgctcgta cgcgtccccg ctcgccgccg tcagctcccg cagccacccc tgctgctggg
     6841 cggtcggctg gttgggcagc tccaccccga gcttcgccgc cacgtcccgg gcccgccggt
     6901 ccaggtccgc gtgcccgacg accaggtggt ccccggccgc cttggtcgcc tcgctggggg
     6961 cccgttcgat cgcctgctgc ccggcgggca gctcccacag tccggcgagc cgcaccttga
     7021 ccaggaagtc ccggtccgtc gcggagagcg ggccccactg ggtggcgacg ctggaggcgt
     7081 tgagatgcgc ctcgccggtg cccgaacggt ccgcgtagga ccagaccggg aacgcgagcg
     7141 cgccgagcgt ggcgaccagc gccgcgatga tgagggccgt tccgttgatg cgtcgcaaca
     7201 aggtgtctcc cgggccgagg cgtgacagcg tcgatcgaac aggcgtcaat caaccgccaa
     7261 tcgaccggct gcgcaggcta cttggcggta atgccgctag ggcagtaccg ggagatacgt
     7321 aagaggaacc agaagtgttc aggtgcgcag cgccgggtgg tcggcgacca ccgtgcagga
     7381 ccccggggcg atctcggtga accccgcgtc ccgcaccacc ggcagcccgc tcaccgtcag
     7441 ctcccgccag cgctccgcac cgggggtggc gacggcgagc gggaacccgg cctcgcgcca
     7501 ggccttgcgc tcggtgtcgc ccagctccca ccaggcgagc tgggcgccat ggccgacctg
     7561 cgccatcgtc ttgcccgccg acatgtccag atccgggttg agccacagca ccgggccggc
     7621 gggatcgggt gcggcgggcg gctcggggtc ctccagatcg gtgcccgaga cctggagctt
     7681 ggccagctcc ctgggccagc cgtccagcgg gatcggcggg aacacccgta cctccgcgcc
     7741 gtcctcgccg gtcaccgtga tgccgtccag cgccgcggcc ttgcgccact ccgcgccgcg
     7801 cgcccggcgc acgaccttcc ggatccgggc gtcctgccag tcccgcatcg ccacggccca
     7861 ctcgccgtcc ccgagggacc gctcgtcgga gaggatcgtc agcaccgcac gggcggcggt
     7921 ccggagcgcg tcggtgcggg cgggcgggtc cgtcttctcc aggtggagca ccagcggcag
     7981 cacgaactgc tgcgcctggt cgcggtcggt cggaccgtcg tggaacgggc tctcggggac
     8041 gggggcgggg gtgtcatcgc tggtcaccgt cccagtctgc caggccgacc ccgtcacctg
     8101 cttggcggaa cgaccgggtc cgggtgagga tgctccgcat gaagagcgat ctcttttcca
     8161 gcgaccacat ggcccagccg gccaccgccc ccggcatgac cctgcagaac accaaatcga
     8221 tcaagtacgc cgtcaacggc gagatgcacg cccggcaggg ctcgatgatc gctttccggg
     8281 gcaacctcca gttcgaacgc aagggccagg gcatcggcgg cctgctcaag cgtgcggtca
     8341 ccggcgaggg cctggccctc atggcggtcc ggggccaggg cgaggcgtgg ttcgcgcacg
     8401 aggcggccaa ctgcttcatc gtcgagctgg accagggcga ctccatcacc atcaacggcc
     8461 gcaacgtcct ttgtttcgac cccacgctct cctacgagat caagacggtg aagggcgccg
     8521 ggatggtcgg cggcggcctc ttcaacagcg tcttcacggg ctacggcaag ctcggcctga
     8581 tgtgcgaggg caccccgatc gtgatccccg tgacggccgc ccagccggtg tacgtggaca
     8641 cggacgcggt cgtcggctgg agccagcagc tcacgaccag cctgaaccgc tcgcagagcg
     8701 tcggctcgat ggtgcgcggc ggatccggcg aggccgtcca gctgatgctc cagggcgagg
     8761 gcttcgtcat cgtgcggccc agcgaggcca ggcccgagaa gacctccaac tgaccctgaa
     8821 cctacggggc gtggggcacc ggtacggcct ccgcggcgcc tgggtgctgc gcggggtcga
     8881 cctcacgctg cccgcgcgga ccctcgtccg ggtcgaaggg ggcaacggcg ccgggaagtc
     8941 gaccctgctg cggctgctcg ccgggatcga cgcccccgcc gaggggcgga tcaccggccg
     9001 tcgggcccgc acggcgtacg tccccgagcg cttcccggcg gcgctgcccc tgaccgccac
     9061 cggctacctc gtccacctcg gccggatcca cggactgcgg agcgccgagg cgaagcggcg
     9121 ggcgggggag tggctgaccc ggttcggggc ggcggaccac gcccgtaccc cgctcgccga
     9181 actctccaag ggcaccagcc agaaggtcgc cgtcgcccag gcgctgctcg ccgaaccgga
     9241 tctgctggtc ctggacgagg cctggaccgg cctcgacacc gccgcccgcg ccgaactcga
     9301 ccgggcggtg gcggaacgcg tcgcggcggg cgccaccgtg gtcttcgtcg accacgaccc
     9361 gcgacgcctc gccggggcgg cggacctcac cctgcggacc gggggcggcg gggtgcaccg
     9421 ggtgacggct ccggcgccga cgggacccgc ctccggcccc cacgtcctga tcgaggccgt
     9481 gggcccgccg ggcgcaccgc tgcccgagga tctgccgggc gacccggccc gggaccggcg
     9541 gaccggcgcg gtccccggcc tcgtacgtct caccgtcccc gccccgtact ccgacgccgt
     9601 cctggcggcc ctgctcacgg cccgtccgcc ctggcacatc cggcaggtcg ccccggcgga
     9661 agcgcacggg ccggccgaag caccggcaca cggggcggcc gaggcaccgg cacacggggc
     9721 ggccgaggca ccggcgtacg ggccgacgcc gaccgggccg gccggtagcc aggtgtccgc
     9781 gaaggcgccc gcgcaggctc ccgtacgggc caggaccccc gaggcccccg aggtgtccga
     9841 agctccatga tcgccctcct ccgctaccag gccgccctgc tcgcccgttc ccagcgctgg
     9901 ctggcgcccc tcctgctgta cgcggccgtc ctcgccgtcg gcgttcggag cgggcagccc
     9961 gtgctgggct cgctcggcgt ggcggccgcc gccgtgctgc ccgtcaccgc gtgggccgtg
    10021 cacctctgcg tcgcccagga gccgcccgcc gcccggacgg tcgcctcggc cgccgcgggg
    10081 ccccggcggg tccacctggc cgcgctgctg accgccacgg gctgtgcggc ggcgctcgcc
    10141 gcggtgaccg gcgccgtcgt cctgttcatc agcagcccgg tcaccgacga cggcgcccgc
    10201 gccgtctccc ggccgtccgc cgcgctcgcc ggactgctcg ccgtcctgtg ctgtgtgctg
    10261 ctgggcgcgg ccgtgggggc gctcagcacc cgcccggtgc cgctcgaccg gggctggtcc
    10321 gtcgcggcgg ccgtgctcgg ctcgcttctc gcgctcgtga ccaccggatc cccggcgcgc
    10381 ctcgcggtga ccgccctggt cacgggatcg cggaccgggg cggtccagcc gtccgtcctg
    10441 cccctgctcg gggcggcggc cctcgccgcg gcgtcggcgt tcttcgtctg ccggatcgcc
    10501 gcccggcgcg ggtgagtagg ctcgtgggcg tggacacgga cacaaacacg gacacggacg
    10561 acacctactg cgagaccccg gggccgatcg caccggaccc gtcgggcggg gcggccgaag
    10621 gccctccgta cgccgagtgc gtgctgtgcc gcgagccgac cgaatacccg gagtcgacga
    10681 agggcgccac gctctgcccg gtctgcgcct ggcaggaggc gggccgcacg gcctgctccg
    10741 gctaggaaga ggcccagggg gtcggacggg gctactttcg cagcacgccc ggggaccggt
    10801 cctccatcaa ctccgacacc ttcacgaagc gatagccgcg ctcgcgcagt tccggcacga
    10861 tccggcgtac ggccgcctcg gtggccgggg cggcgctgcg ggtgcagtgc atcaccacga
    10921 gggagcccgg ccgcaccccg tccagcacct gctcggccac cgcgtccgcg tccgtggcga
    10981 aggcgtcgcc gctgaccacg tcccactgca cggccgtcac gttcgccggg gccagcgcgc
    11041 gcagtgccgt gtcgtcgtaa cagccgccgg ggaagcggaa gtacggcacc acgttccgcg
    11101 cccccgcctt ccggaacgcc gagaaggcac gttccacctc gcgggccatc gcggccctgc
    11161 cgaccaccgg caggccgtag cagggggagc tgaaggcgtg gtggctgtag gagtggttgg
    11221 cgatctcgaa catcgggtcc gtgccgatgg ccttggcctg cgccggatac tcctcggccc
    11281 agcgcccggt catgaatacg gtggagggca ccttcagccg acgcagcag
//
