LOCUS       NZ_LIVW01000003        10798 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02115 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LIVW01000003
VERSION     NZ_LIVW01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02115
  ORGANISM  Streptomyces sp. CB02115
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1032236)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032236)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905645.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,651
            CDSs (total)                      :: 7,572
            Genes (coding)                    :: 7,475
            CDSs (with protein)               :: 7,475
            Genes (RNA)                       :: 79
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 79 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 440361
            Orig. end                         :: 451159
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVW01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5798
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(240..255)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.98"
                     /tool="antismash"
     gene            705..1679
                     /locus_tag="AMK28_RS19365"
                     /old_locus_tag="AMK28_19120"
     CDS             705..1679
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100029.1"
                     /locus_tag="AMK28_RS19365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19120"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_073742523.1"
                     /transl_table=11
                     /translation="MTDLPPPITPFLEPAAKELCEATDPHPRIYEVPPEKGRDILLGLQ
                     SDRSVPRPEVDEEWVDVDAGQWGTVRTRIIRPKGATGQLPVVFYIHGAGWVFGDDRTHD
                     RLFRELAVGAGAAGVFPVYDRAPEAKYPTQVEQNYAVGQWVLEHGAQHGLDTSRIAVTG
                     ESVGGCMSAVFALMNKERGGIDLKAQVLLYPVTDADFSTPSYLQFAEGYYLTRDGMKWF
                     WDAYTGNLAHRTEAYASPLRSSLEQLRGLPTTLVITDEADVLRDEGEKYANRLREAGVD
                     VTSVRVAGMVHDFLLLDSLRDTRAANVARKLAVDALRTALHDD"
     gene            complement(1746..3053)
                     /locus_tag="AMK28_RS19370"
                     /old_locus_tag="AMK28_19125"
     CDS             complement(1746..3053)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513141.1"
                     /locus_tag="AMK28_RS19370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19125"
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="WP_073742524.1"
                     /transl_table=11
                     /translation="MTSELFSPLHLRSGHVLKNRIAKAAMEENMAGYGQLSDRRLLSLH
                     RRWAAGGAGPLITGNVMVHAEALTGPGGVVLDEIAPLRPFTEWAEAGRSGGGAVWMQIN
                     HPGRQVASGMPGVVWGPSGIGVDLGRHSGRFGRPTAMTPARIDATVTRFAVTAHRAEQA
                     GFDGVEVHAAHGYLLSQFLSPLVNKHTDRWGGPLEDRARMLLDVVRAVRAAVSPSFAVA
                     VKLNSAQFQRGGFDADDARRVIGMLQPLGVDLVELSGGSYESPAMTGRSGDARTQAREA
                     YFLDLAKDLVKTSPLPLMLTGGITRRATAERVRDSGVAVVGMGTALAVTPDLPDRWRNH
                     READRRMAPVTWSDKALASAAGMARVSHQMGRIARGSDARPRIHPAYTLLSEQWKQRGA
                     LRRYRAWLAASQGAEAVNRPAQPDPFSAGIRSSASS"
     misc_feature    3083..3101
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.37"
                     /tool="antismash"
     misc_feature    complement(3087..3105)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.54"
                     /tool="antismash"
     gene            3148..3780
                     /locus_tag="AMK28_RS19375"
                     /old_locus_tag="AMK28_19130"
     CDS             3148..3780
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122776.1"
                     /locus_tag="AMK28_RS19375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19130"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_073742525.1"
                     /transl_table=11
                     /translation="MPQASTYHHGDLRAACLRAARELLEEDGSAGLSLRAVARRAGVSA
                     TAPYRHFADREALVSAVAAQGYGELAGHLAEAHPAPSTPDELAEVAVAYVRFALDHPAL
                     FRAMFAEPCDPTGEERVAATAAIPEYVRGIVRATFPGVDADALSTTVWALVHGLAFLHL
                     DGKLDSSTPGTVAAQVESAVHALFDASPAMSRAAAAPAGDQGLSGGS"
     gene            3928..5001
                     /locus_tag="AMK28_RS19380"
                     /old_locus_tag="AMK28_19135"
     CDS             3928..5001
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="AMK28_RS19380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19135"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_073742526.1"
                     /transl_table=11
                     /translation="MPDAVPDPVSVQPVVAPLTSAALFLVGTIEPGGESAVRDVLPDLA
                     AVARSLGFRYPDAGLACVAGFGSSAWDRLFAGPRPAHLHPFPELRGPLHHAPATPGDVL
                     LHIRAERMDVCYAWAAQLLDKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     SAALVGAGDDPDFEGGSYVVVQKYLHDLTSWNALSVEEQERVIGRTKLDDVEFPDDEKP
                     ADSHLALNTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGSLFFAPRADFLDAPPPPPVLTSSGDRPEPVPAPVRQEPVAA
                     GADHGSLRIGSLQESAQ"
     gene            5001..5798
                     /locus_tag="AMK28_RS19385"
                     /old_locus_tag="AMK28_19140"
     CDS             5001..5798
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="AMK28_RS19385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19140"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_073742527.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.5e-89, bitscore:
                     295.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLTEIDPPTPDVVARARTSMPVIEWRVPFTVTRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYAAAGITGLRDGSSHDPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSIHLYFQQAFTFRMLTPEAVVGLIA"
     gene            5889..7271
                     /locus_tag="AMK28_RS19390"
                     /old_locus_tag="AMK28_19145"
     CDS             5889..7271
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955647.1"
                     /locus_tag="AMK28_RS19390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19145"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_073742528.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 1.7e-37, bitscore:
                     124.8, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 5.5e-09,
                     bitscore: 31.9, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSAHHQGGTEPPAARFDAVVRAAVGQGLLGEGCPVAGFIDT
                     EGVRASVDALHDAFAATPEVLHTFAAKACSLVPVLRLLADCGMGCEVASPGELRLAVEA
                     GFAASRVVLDSPAKTREEIRLSLALGVAVNADSLDELRRIDALRNPGTASVLGLRVNPQ
                     VGGGSIGPMSTATDTSKFGVALRDPGAREAIVEAFRERPWLTRLHTHVGSQGCPLELIA
                     DGVARTYELAEEINATLGVRRVTSLDIGGGLPVNFADESVRPGYADYVAALTAAAPGLF
                     SGRYSLVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVLMPGAWPLR
                     IGAFGPDGTPKTGPSLAQDIAGPCCFAGDVVAYGRELPELAEDDVVVLYDTGAYYFSTP
                     WAYNSLPRPAVYGFRAVAGPEHGAGHDGPVTFATVRDAQSLDDIAAESGLAHADALIGR
                     DG"
     gene            7757..8527
                     /locus_tag="AMK28_RS19400"
                     /old_locus_tag="AMK28_19150"
     CDS             7757..8527
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="AMK28_RS19400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19150"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_073742530.1"
                     /sec_met_domain="PIG-L (E-value: 8e-21, bitscore: 70.8,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLPSVLAVHAHPDDESLFCGGVLAQHAAADARTAVVTATWAEGT
                     HRAVELAGALDALGAGAPRLLGYADARVPESAPGRPRFLDAPLDEAVEAVVGHIRDVRP
                     QIVITVDAYGGMTGHPDHVHAHRVTALAVRAAGLPRFCPGSGDPWQPSALHLATHPRSA
                     AVPVGGRLARPGTPADALYCSEDAWITTTVDVGPWFPQKLAAILAHRSEVERGAAPGRI
                     AALPPAVQREVLGTEWYIRHDLAPRSGSATELSA"
     gene            complement(8648..9001)
                     /locus_tag="AMK28_RS19405"
                     /old_locus_tag="AMK28_19155"
     CDS             complement(8648..9001)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="AMK28_RS19405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19155"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_007456260.1"
                     /transl_table=11
                     /translation="MAEKAAVEIRDDREHGSLVAYEDGKPAGTIAYFVMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFYTMAAREGVPVVPLCPYAAKWAQRHPDEAPEAPAELVR
                     EAERQLRNHPELF"
     gene            complement(9063..9488)
                     /gene="panD"
                     /locus_tag="AMK28_RS19410"
                     /old_locus_tag="AMK28_19160"
     CDS             complement(9063..9488)
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="AMK28_RS19410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19160"
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_050361929.1"
                     /transl_table=11
                     /translation="MLRTMFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVALGADASAPVPGTRTERSPQAVVAGG"
     misc_feature    9470..9484
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.95"
                     /tool="antismash"
     gene            9764..10651
                     /locus_tag="AMK28_RS19415"
                     /old_locus_tag="AMK28_19165"
     CDS             9764..10651
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740738.1"
                     /locus_tag="AMK28_RS19415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_19165"
                     /product="DUF3626 domain-containing protein"
                     /protein_id="WP_073742531.1"
                     /transl_table=11
                     /translation="MNSTASSTAQERALRHVTELASGPPVDPRLRVTLNFHPDRLLHGR
                     PILDALAEDGVYRSQFVTGTSNGGLTAYPGGDRWGWESRIFGGAYDEAPAHERPVYGAL
                     NFRRKPVGAAPRFGSGHFRLAAETLARTTFCYPDSFLEPADFGVAASMGLLELALADRQ
                     DDLDDYIEAQVHGPVRPRCHVEALVLDPCYRGTAVEDAAMRLGCPVEWHLGFRLGVEEL
                     RRHPGYRGQEHVDLGTRIAVDGVLDPRLVGDAARAGRHDPQAVKKVWHYLARFGAPRRD
                     ADEAASASPMEERV"
ORIGIN
        1 ctcgaagatc gtcgcctaca cccagggctt ccacgagatc gcggccggca gcgagcggta
       61 cgactggaac atcgacctcg gcaaggtcgc ctcgatctgg cgcggcggat gcatcatccg
      121 ggccgcgttc ctcgacagga tcaccagcgc ctacgaagcc cggccggggc tgcccagcct
      181 cctcgccgac aagagtttcg cggaggagat cgccgccgcg cagggcgact ggcgcaccgt
      241 gatcgccacc gccgtcaccc ggggcgtccc caccccggcc ttcgccgcca ccctcgccta
      301 ctacgactcg ctccgcgccg aacgcctccc cgccgccctc acccaggggc agcgcgacta
      361 cttcggcgca cacacctacc accgcaccga ccgcgagggc acgttccaca ctctctgggg
      421 cggcgacagg accgaggtcg aaagctgacg cgccttcgag actcgcgctc cggtcgatcc
      481 gaccgggtgg gtgaggatgc ccgcgtaccc gcggcctcaa tgttcgcaat gcatacatca
      541 ggtaccggcg tgtgcgacgc caacatccga ggcgcccggc gcggttgcgc tccacgggcg
      601 gggggtgagg atggaacgag ggggcggacc cgggggcgaa cagcagcggc ggcccgcgaa
      661 gccgcgcgaa cagggtgccc gcgcgcccgc gacatggagc cgacatgacc gatcttcctc
      721 ctccgatcac gcccttcctc gaacccgccg cgaaggaact gtgcgaggcc acggacccgc
      781 atccgcggat ctacgaggtc cccccggaaa agggccgcga catcctgctc ggcctgcaga
      841 gcgaccggag cgtgccccgc cccgaggtcg acgaggagtg ggtcgacgtg gacgcgggcc
      901 agtggggcac ggtccgcacc cgcatcatcc ggcccaaggg ggccaccgga cagctgccgg
      961 tggtgttcta catccacggc gccggctggg tcttcggcga cgacaggacg cacgaccgcc
     1021 tcttccgcga actcgccgtg ggcgccgggg cggcgggcgt cttccccgtc tacgaccgcg
     1081 cacccgaggc gaagtacccc acccaggtcg aacagaacta cgcggtgggc cagtgggtgc
     1141 tggaacacgg cgcgcagcac ggcctggaca cctcccggat cgccgtcacc ggcgagtcgg
     1201 tcggcggctg catgtccgcc gtcttcgcgc tgatgaacaa ggagcgcggc ggcatcgacc
     1261 tcaaggcgca ggtcctgctg tacccggtca ccgacgccga cttcagcact ccttcgtacc
     1321 tccagttcgc cgagggctac tacctcaccc gggacggcat gaagtggttc tgggacgcct
     1381 acaccggcaa cctcgcccac cgcacggagg cgtacgcctc accgctccgg tcctccctgg
     1441 agcagctccg gggcctgccc accacgctgg tcatcaccga cgaggccgac gtcctgcgcg
     1501 acgagggcga gaagtacgcc aacaggctgc gcgaggcagg cgtggacgtg acctccgtcc
     1561 gcgtggcggg catggtccac gacttcctcc tcctggacag cctgcgcgac acccgcgccg
     1621 cgaacgtcgc ccgcaagctc gccgtcgacg ccctgcgaac agccctgcac gacgactgag
     1681 gccgccccac cgcgccgacg gcccggactc ctcggtaccg accaccacga tccggtgccg
     1741 gcccgtcagg acgaggcgct ggacctgatg ccggccgaga acgggtccgg ctgcgcgggc
     1801 cggttcaccg cctcggcacc ctgcgaggcc gccagccagg cgcggtagcg gcgcagcgcc
     1861 ccacgctgct tccactgctc ggagagcagg gtgtacgccg gatggatccg gggcctcgca
     1921 tcgcttccgc gcgcgatgcg gcccatctgg tggctgaccc gcgccatgcc cgccgccgag
     1981 gcaagggcct tgtcggacca ggtcaccggt gccatccgcc ggtcggcctc acggtggttc
     2041 ctccagcggt cgggcaggtc cggggtgaca gcgagggcgg tgcccatgcc gacgaccgcg
     2101 acgccgctgt cccgtactcg ttcggcggtg gcgcgccgcg tgattccgcc ggtgagcatc
     2161 agcggaaggg gactggtctt caccaggtcc ttcgccaggt ccaggaagta ggcctcgcgc
     2221 gcctgcgtac gcgcgtcgcc ggaacggccg gtcatcgccg ggctctcgta gctccctccg
     2281 gaaagctcga ccaggtcgac gcccagaggt tggagcatcc cgatgacccg gcgggcgtcg
     2341 tcggcatcga atccaccgcg ctggaattgg gcggagttga gcttcaccgc gaccgcgaag
     2401 gacggcgaga cggccgcccg gacggcgcgg acgacatcga gcagcatgcg cgcgcggtcc
     2461 tccagcggcc cgccccaccg atcggtgtgc ttgttgacca ggggtgacag gaactgcgac
     2521 agcagatagc cgtgggcggc atggacctcc acgccgtcga aaccggcctg ctcggcacgg
     2581 tgggccgtga cggcgaaccg ggtgacggtg gcatcgatcc gggccggggt catggcggtc
     2641 ggacggccga agcgaccgct gtgccggccg aggtcgacgc cgatgccgga gggaccccac
     2701 accacgccgg gcatgccgga ggccacctgc cggccggggt ggttgatctg catccacacc
     2761 gcgccgcctc cggacctgcc ggcctcggcc cactccgtga acggccgcaa cggcgcgatc
     2821 tcgtcgagca cgacaccccc ggggcccgtc agcgcctccg cgtgcaccat gacgttcccg
     2881 gtgatcagcg gcccggcgcc accggcggcc cagcgccggt ggagcgacag caaccgacga
     2941 tcggacagtt ggccatagcc cgccatgttc tcctccatcg ccgccttggc gatccggttc
     3001 ttcagcacat gtccggaacg caggtgcagc ggcgagaaca gctcgctggt catcccggcc
     3061 ccctctccga tctcggcccc ttacgatgta tgcagcattc acatgagacg acaatgtgag
     3121 caccgtgaac attgaggagt gtgacctgtg ccgcaggcca gcacgtacca ccacggcgat
     3181 ctgcgcgccg cctgcctgcg tgccgcacgc gaactcctgg aggaggacgg cagcgccggc
     3241 ctttcgctgc gcgcggtggc ccggcgcgcc ggagtgtcgg cgacggcccc ctaccgccac
     3301 ttcgccgacc gcgaggctct cgtctccgcg gtggccgccc aggggtacgg cgagctcgcc
     3361 ggacacctgg ccgaggccca tcccgccccc tcgacgcccg acgaactcgc cgaggtcgcg
     3421 gtcgcctacg tccggttcgc actcgatcac ccggccctgt tccgggcgat gttcgccgag
     3481 ccctgcgacc cgaccggcga agaacgggtc gccgccaccg ccgccatccc ggagtacgtc
     3541 cgcggcatcg tccgcgccac cttccccggc gtggacgcgg acgcgctgtc gaccacggtg
     3601 tgggccctcg tccacggtct cgcgttcctg cacctcgacg gcaagctcga cagctccacc
     3661 cccgggaccg tcgcagccca ggtcgaatcc gccgtccacg cgctgttcga cgcctccccc
     3721 gcgatgtcac gggcggctgc cgcaccggcc ggcgaccagg gcctgtccgg cggatcctga
     3781 ccgggtccgc gcgctccccc ggccgggcgc tcccggcgtg cggcagggga cgggacgtgg
     3841 gttagtggct gcgagccctg ttcgtcgggg tccgtcgacc gccggctgct caccgccgtc
     3901 cgtacaccgt cgtaatggag tgcgcgcatg cccgacgccg tccccgatcc cgtctccgtc
     3961 cagcccgtcg tggcgccgtt gaccagcgcg gcactgttcc tggtcggcac gatcgagccg
     4021 ggcggcgaga gcgcggtgcg ggacgtgctg ccggatctcg cggccgtcgc ccggtcgctc
     4081 ggcttccggt acccggacgc cggtctcgcc tgtgtggcgg ggttcggctc cagcgcctgg
     4141 gaccggctgt tcgccgggcc gcgcccggct cacctgcacc cgttcccgga gctgcgaggg
     4201 cctcttcacc acgccccggc gacccccggc gacgtgctgc tgcacatccg ggccgagcgg
     4261 atggacgtct gctacgcgtg ggcggcccag ttgctcgaca agctcggcgg agccctgcgg
     4321 atcgtggacg agacgcacgg cttccgctac ctggaccacc gggacctgct cggcttcgtg
     4381 gacggtacgg agaacccggt gggcgacgac gcccgttcgg cggcgctcgt cggggccggg
     4441 gacgacccgg acttcgaggg cggcagttac gtcgtcgtac agaagtacct gcacgacctg
     4501 acgtcctgga acgcgctctc cgtggaggag caggaacgcg tcatcggccg caccaagctg
     4561 gacgacgtgg agttccccga cgacgagaag cccgccgact cccacctcgc gctcaacacg
     4621 atcaccgacc cggacggcac cgaacgggac atcctgcggg cgaacatgcc gttcggaagc
     4681 ttcgggaagg gtgagttcgg tacgtacttc atcgggtacg ccgccgatcc cgacgtgacc
     4741 gagcggatgc tccgcaacat gttcctcggc gatccgcccg ggacgcacga ccgcatcctc
     4801 gacttctcca cggcggtcac cggttccctc ttcttcgccc cccgcgccga cttcctcgac
     4861 gctccgccgc ccccgcccgt cctgaccagc tccggggacc ggccggaacc ggtgcccgca
     4921 ccggtccggc aggagcctgt cgcggccggg gcggaccacg gttcgctgcg catcggcagc
     4981 ctgcaagaaa gcgcccagtg atgaacaatc tgcatcgcga actcgctccg gtcaccccgt
     5041 ccgcctggga cgagatcgag gaggaggcac ggcgcacctt ccgccgccat gtcgccggcc
     5101 gccgtgtcgt cgacgtgtcc gatccggacg gtccgacgct ggccgccgtc ggcgacggcc
     5161 atctgaccga aatcgatccg cccaccccgg acgtggtggc ccgggcccgc acgtcgatgc
     5221 cggtcatcga gtggcgggtg ccgttcacgg tgacccgcca ggccgtggac gacgtggagc
     5281 gcggctccgc cgacagcgac tggcagcccg tcaaggacgc ggcacgcacc tgtgcgttcg
     5341 ccgaggacat ggcgatcatc gacgggtacg cggcggccgg gatcaccgga ctgcgggacg
     5401 gctcctcgca cgatccgctg cctctgcccg ccgacgcgcg ggactacccg gtggcggtca
     5461 gccaggccgt gacgcggctg cggctggccg gtgtggacgg cccgtaccgg ctgctgctcg
     5521 gcgcggacgc gttcaccgag gccgcggaga cctccgacca cggctatccg gtcaagacgc
     5581 acttgagccg cctggtggac gacgagatcc tgtgggcgcc cgcggtcaag ggcggggtgc
     5641 tcctgtcgac ccggggcggg gacttcgaac tctgcctggg ccaggacctg tcgatcggct
     5701 acgcggacca cgacgccacg agcatccacc tctacttcca gcaggccttc accttccgga
     5761 tgctgacacc ggaggccgtg gtcggcctga tcgcctgatc ccgggggcgc tcaccggcgc
     5821 gtggccgggc gaccgagcgc ccccgttccg ccgaagaccc gggggcagcc atcagggact
     5881 ctggccgtat gacgcagacg acttccgcgc accaccaggg cggcacggaa ccgccggcag
     5941 cccgcttcga cgccgtcgtc cgggcggccg tcggccaggg gctgctgggc gagggctgcc
     6001 cggtcgccgg attcatcgac acggagggcg tgcgcgcctc cgtcgacgcc ctgcacgacg
     6061 ccttcgccgc cacgcccgag gtgctgcaca cgttcgcggc caaggcctgc tccctcgtcc
     6121 cggtgctgcg gctgctcgcc gactgcggca tgggatgcga ggtggcgagc cccggtgaac
     6181 tgcggctggc cgtggaggcc gggttcgccg cgtcccgtgt cgtcctggac tcccccgcca
     6241 agacccgcga ggagatccgg ctgtcgctcg ccctcggcgt ggccgtcaac gccgacagcc
     6301 tcgacgaact gcgccgcatc gacgccctcc gtaacccggg caccgcctcg gtcctcggac
     6361 tgcgggtcaa ccctcaagtg ggcggtggct cgatcgggcc gatgagcacg gcgacggaca
     6421 cgtcgaaatt cggggtcgcg ctgcgcgacc cgggggcccg cgaggcaatc gtggaggcct
     6481 tccgggagcg gccgtggctg acccggctgc acacccatgt gggctcccag ggctgcccgt
     6541 tggagctgat cgcggacggg gtggcacgga cgtacgagct ggccgaggag atcaacgcca
     6601 cgctgggcgt ccggcgggtc accagcctgg acatcggcgg cggtctcccg gtcaacttcg
     6661 ccgacgagtc ggtgcgcccc ggttacgcgg actacgtggc ggccctgacg gcggccgccc
     6721 ccggactctt ctccggccgc tactccctgg tcaccgagtt cggccggtcg ctgctggcga
     6781 agaacgggtt catcggggcc cgggtggagt acacgaagga tgccgggggc cgccggatcg
     6841 cgctcaccca cgcgggagcc cagaccgcca cccgtaccgt cctcatgccc ggcgcctggc
     6901 ccttgcggat cggggcgttc ggcccggacg gcactcccaa gaccgggccg tcgctggcgc
     6961 aggacatcgc ggggccgtgc tgcttcgcgg gggacgtggt ggcgtacggc agggagctgc
     7021 cggagctggc cgaggacgat gtggtcgtgc tgtacgacac gggggcgtac tacttctcca
     7081 ctccgtgggc gtacaacagc ctgccccgtc ccgcggtgta cggattccgc gcggtggcgg
     7141 ggccggagca cggggcgggg cacgacggac cggtgacgtt cgcgacggtg cgggacgcgc
     7201 agtcgctgga cgacatcgcg gcggagagcg gcctcgcgca cgcggacgcg ctgatcgggc
     7261 gggacggctg aacgggcggg ccgggccctg gcaccggccg gaaccggacc cggccgccgg
     7321 tcacgtcccg gcgggctgcg gctcgggctc cggatccggt acggggtccg ggcccggcgc
     7381 gggcggctgc gggatcgggt ccggcgcggg acccggcggt accggccccg gacccggcgg
     7441 cacgggcggc ggacccggca ccggagtggg cgggcccggt acgggcggag gcgtcggcga
     7501 gggcggtacg gggtcggggt acgggtgggt catcacgatc ctccacaacg acacgaaagg
     7561 gacagatgga gctttcctcc accgtctccc ggagcggctg cccgcgcctg ccgagtccac
     7621 acctgcgccg ggcgacagcc gcgcgggccg acgtgttccg ggccgtccgg agtcggcagg
     7681 actccggcga ggcgcccgga cctgaccggg tccgtccgga tccgcgaccg gcgccgtcga
     7741 tgcgaaaggc gtccccatgc ccctgcccag cgttctcgcc gtccacgccc atccggacga
     7801 cgagtccctg ttctgcggag gcgtcctcgc ccagcacgcg gccgccgacg cccgcaccgc
     7861 ggtggtcacg gcgacctggg cggagggcac gcacagggcc gtcgagctcg ccggggccct
     7921 cgacgccctg ggggcgggcg ccccacggct gctcggctac gcggacgccc gtgtgccgga
     7981 atcggcccct gggcggcccc ggttcctcga cgcgccgctg gacgaggccg tggaggccgt
     8041 cgtcggccat atccgtgacg tccggccgca gatcgtgatc acggtggacg cctacggagg
     8101 catgaccggc caccccgacc acgtacacgc ccaccgggtc accgcgctgg cggtccgggc
     8161 cgccggcctc ccccgtttct gtccaggatc cggtgatccc tggcagccga gcgccctcca
     8221 cctcgccacg cacccccgct cggcagccgt cccggtgggc gggcggctgg cccggccggg
     8281 aaccccggcc gacgcgctgt actgctcgga ggacgcgtgg atcaccacga ccgtcgacgt
     8341 gggcccctgg tttccgcaga agctcgccgc gatcctcgcg caccgcagcg aggtggaacg
     8401 aggagcggcg cccggccgga tcgccgctct gcctccggca gttcagcgcg aggtgctggg
     8461 caccgagtgg tatatccgcc acgacctcgc accccggagc gggtcggcca cggaactgag
     8521 cgcgtgaaat gcccgggggc ggccggggcc ggtagcccct gcgcgggcag cggccgcgat
     8581 cgcactacgg ccgctacagc gactacccgc ccctagccct gcccctgccc ctgcccctcc
     8641 cctgccgtca gaacagctcg gggtggttcc tgagctgccg ctccgcctcc cggacgagct
     8701 cggccggtgc ttcgggcgcc tcgtcggggt gacgctgcgc ccacttcgcc gcgtacgggc
     8761 acagcggaac gacggggact ccctcgcggg ccgccatggt gtagaactcc cggacgagcg
     8821 cacccgcgat gcccttgccc tcgtgttcgg gccggacgac ggtgtgcacg gccaccagtg
     8881 cgccgggggc cgggtccatg acgaagtacg cgatggtccc ggccggcttg ccgtcctcgt
     8941 acgccacgag gctgccgtgc tcccggtcgt ccctgatctc taccgctgcc ttctcggcca
     9001 tggcccctgc cttctcgttc gtggacgtgg acatcacccg tccggaaaca cggacagcga
     9061 cctcagccgc ccgcgacgac ggcctgcggg ctccgctccg tacgggtccc gggcaccggc
     9121 gccgaggcgt cggcgccgag cgccacgatc cggttgtccg cgtccacgtg cacgacccgc
     9181 ggcacgaggg cacgggcctc cgcgtcgtcg acctgtgcgt agctgatcag gatgaccagg
     9241 tcaccggggt gcacgaggtg tgcggccgcg ccgttgatcc cgatgacgcc ggagccgcgc
     9301 tcgccctcga tgacgtaagt ctccaggcgg gcaccgttgt cgatgtcaac gatatggacc
     9361 agctcgccgg gcagcaggtc ggcggcctcc atcagggcgg cgtcgatcgt cacggaaccg
     9421 acgtagtgca ggtcggcctg ggtgacggtg gcccggtgga tcttggactt gaacatcgtg
     9481 cgcagcactt gggactcctc cgagacggct ccctgcctgc tttgcgcagg tcaagggcgc
     9541 cctcgacttt acaccggcac acgccgaacg aggcttgtga ggaacatcgc tccggttcac
     9601 ggaacacggc ccccttggcc ttccagagtc ggtgggacct cctccagcgc atactcgacc
     9661 cgaacttcgt ctcgatcggc gatcgacgct ccgagacgcc gggacgggct gccggtggac
     9721 gatccgggtg cgccggttga tcacgtcggg aaacactgtg cggatgaact ccacagcctc
     9781 ctcaacagcg caggaacggg cccttcggca tgtcacggaa ctggcgtcgg gccctccggt
     9841 ggacccgagg ctgcgggtga cactcaactt tcacccggac cgcctgctgc acggaaggcc
     9901 gatcctggac gccctggccg aggacggcgt ctaccgctcg cagttcgtca cgggaaccag
     9961 caacggcgga ctgaccgcgt atcccggcgg ggaccggtgg ggctgggaga gccgcatctt
    10021 cggcggtgca tacgacgaag cacctgctca tgaacggccg gtctacggag cgttgaactt
    10081 ccgtcgcaag cccgtcggcg cggcaccgcg attcggctcc ggccacttcc ggctggctgc
    10141 cgaaacactg gcgcggacca ctttctgcta cccggacagc ttcctcgaac cagcggattt
    10201 cggtgtcgcg gccagcatgg ggcttcttga gcttgccctc gccgatcgcc aggacgacct
    10261 tgacgactac atcgaggcgc aggtacatgg gccggtgcgg ccgagatgtc acgtggaggc
    10321 gctggtcctg gacccctgct accggggcac ggctgtcgag gacgcggcca tgcgtcttgg
    10381 ctgccccgtg gagtggcacc tcggtttccg gctcggcgtc gaggaactcc gccgccatcc
    10441 tggctaccgc ggccaggagc acgtcgacct gggcacgcgg atcgcggtcg acggcgtact
    10501 cgatccccgc ctcgtcgggg atgccgcacg tgcaggccgc catgaccccc aggcggtcaa
    10561 gaaggtgtgg cactacctcg cgcgtttcgg cgcgccccgg agggacgctg acgaagcggc
    10621 gagtgcgtca ccgatggagg agcgtgtgtg agacgggtgg tggttcgtga gcggagcgca
    10681 ccttcatgtc tcgtccggta ctcggcccgg tacgtcgggc cctgccagcc gatcccgtac
    10741 gccggaccga gcgggcgtac cgggtcgccc tgctcgcgcg gaacgcgtcg gggtgggc
//
