LOCUS       NZ_LIVW01000002        10399 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02115 scaffold2, whole genome shotgun sequence.
ACCESSION   NZ_LIVW01000002
VERSION     NZ_LIVW01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02115
  ORGANISM  Streptomyces sp. CB02115
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1550960)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550960)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905645.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,651
            CDSs (total)                      :: 7,572
            Genes (coding)                    :: 7,475
            CDSs (with protein)               :: 7,475
            Genes (RNA)                       :: 79
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 79 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1470201
            Orig. end                         :: 1480600
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVW01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    135..148
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.28"
                     /tool="antismash"
     gene            184..1242
                     /locus_tag="AMK28_RS17150"
                     /old_locus_tag="AMK28_16935"
     CDS             184..1242
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 2.2e-25.
                     Score: 81.3. Matches aSDomain:
                     nrpspksdomains_AMK28_RS17150_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381431.1"
                     /locus_tag="AMK28_RS17150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16935"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073742082.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-18, bitscore:
                     63.3, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 9.2e-06, bitscore:
                     21.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIQAAAVEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRVGAGSFDAVEAARAGFARLAGVRPDRVATGSSVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRMRYAPLDGLAAAVRPSTSLVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDGTQAAGWFPLEAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFA
                     GWVSAGAPWTSNYGPLERLAPTARGFDEPPAFLSYHGAEHSLDLLAEVGADALYAHATG
                     LAARLRAGLAKLGHATVPGESAIVSVPGLESRQSDLVEAGVAVSAPAGNLRISCHLYNT
                     EADVDRVLEVLA"
     aSDomain        241..1224
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK28_RS17150_Aminotran_5.1"
                     /evalue="2.20E-25"
                     /label="AMK28_RS17150_Aminotran_5.1"
                     /locus_tag="AMK28_RS17150"
                     /protein_end="347"
                     /protein_start="19"
                     /score="81.3"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRVGAGSFDAVEAARAG
                     FARLAGVRPDRVATGSSVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRMRY
                     APLDGLAAAVRPSTSLVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDGTQAAGWFPL
                     EAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFAGWVSAGAPWTSNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLDLLAEVGADALYAHATGLAARLRAGLAKLGHATVPG
                     ESAIVSVPGLESRQSDLVEAGVAVSAPAGNLRISCHLYNTEADVDRV"
     gene            complement(1259..1765)
                     /locus_tag="AMK28_RS17155"
                     /old_locus_tag="AMK28_16940"
     CDS             complement(1259..1765)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007450490.1"
                     /locus_tag="AMK28_RS17155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16940"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_050358211.1"
                     /transl_table=11
                     /translation="MTTGRATPAEDACTNALPMAALSGPVSHAVSRVARLHRTAAGKAL
                     KGVDLYPGQELLMMHLWDRGAVRQTELIRALDLDPSTVTKMLQRLEQTGHVRRRPDPAD
                     RRAVLVEATDESCALHTAVRDAWGSLEEHTLAGLDPAERAELVRLLAKVEENLCRETAD
                     CPDRC"
     gene            1917..2987
                     /locus_tag="AMK28_RS17160"
                     /old_locus_tag="AMK28_16945"
     CDS             1917..2987
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="AMK28_RS17160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16945"
                     /product="alkene reductase"
                     /protein_id="WP_073742083.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLSGAQLANRIALAPMTRSRAGEGGTATELTATYYAQ
                     RASAGLVITEGIQPSVVGQGYPTTPGLHSAEQVASWRKVTDAVHAEGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVSASPVKAEGQVYTHVGPKDFVEPRELTDAEIHATVADFTAASRNA
                     VEAGFDGVELHGANGYLIQQFLAPNTNRRTDAWGGSDEARIRFAVEVVKAVAAEIGPER
                     TALRISPGNPYNDIDEPAPEATYTALVQAIEPLGIAYLHILEAAPIRELTLALRKAFSG
                     TVVLNVHSDGATGPGDHTVIDDGIADLISYGALFLANPDLPARLRAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDSV"
     gene            complement(2963..4000)
                     /locus_tag="AMK28_RS17165"
                     /old_locus_tag="AMK28_16950"
     CDS             complement(2963..4000)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="AMK28_RS17165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16950"
                     /product="VOC family protein"
                     /protein_id="WP_073742084.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADAYALGCFWAQVLGAPLSDEDFPGDPEALVEA
                     PGAAILFVRVDEKKQTKNRVHLDIQPQDRSRDEEVERLLALGATLVGDHRRPNGRGWAT
                     LADPEGNEFCVECSAAERAALTGTRLPVTADDVTSAVRLAVDVLAGAPADRWDAPAGSL
                     DWTCWETVEHLSDDLFAYAVQLGPRTPPLDRDVPYRWAPERQGGPYNAVFADRDAGPAG
                     LLATLEASGALLASMARTTPPEVRSYHGYGISDPEGFAAMGVVETLVHTYDLAEGLGLD
                     WSPSPALCDRVLARLFPDAPAGGDRWTVLLWATGRAELPDHPRRTSWRWDGRPREDAQT
                     ESSAG"
     gene            complement(4101..4994)
                     /gene="thpD"
                     /locus_tag="AMK28_RS17170"
                     /old_locus_tag="AMK28_16955"
     CDS             complement(4101..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="AMK28_RS17170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16955"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_056706218.1"
                     /sec_met_domain="PhyH (E-value: 3.4e-38, bitscore: 128.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAAKDLQGY
                     EHDGFLTVDQLIAPDEVAVYQAELNRLISDPAVRADERSIVEKQSQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="AMK28_RS17175"
                     /old_locus_tag="AMK28_16960"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="AMK28_RS17175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16960"
                     /product="ectoine synthase"
                     /protein_id="WP_007450483.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-54, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(5486..6748)
                     /gene="ectB"
                     /locus_tag="AMK28_RS17180"
                     /old_locus_tag="AMK28_16965"
     CDS             complement(5486..6748)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-363). E-value: 2.2e-71.
                     Score: 232.6. Matches aSDomain:
                     nrpspksdomains_AMK28_RS17180_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="AMK28_RS17180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16965"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073742085.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.7e-77, bitscore:
                     255.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRSQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFEDKARASAVCARAFQLGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        complement(5660..6661)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK28_RS17180_Aminotran_3.1"
                     /evalue="2.20E-71"
                     /label="AMK28_RS17180_Aminotran_3.1"
                     /locus_tag="AMK28_RS17180"
                     /protein_end="363"
                     /protein_start="29"
                     /score="232.6"
                     /tool="antismash"
                     /translation="DRSQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIER
                     DGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGRT
                     GGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFVT
                     AAAALDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFE"
     gene            complement(6909..7439)
                     /gene="ectA"
                     /locus_tag="AMK28_RS17185"
                     /old_locus_tag="AMK28_16970"
     CDS             complement(6909..7439)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="AMK28_RS17185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16970"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_073742086.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSAVARGENGEPIAFVTGYIRPDRPQTLVVWQVAVDQAHRGKGLAAAL
                     LDSLTARVAADQGLSSVETTVTPDNTASDRLFTSYAQRHDVALEKEVLFDGELFPEETH
                     LPEVLYRIGPFAT"
     misc_feature    7450..7464
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7464..7476)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7595..7610)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7605..7620
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7605..7622)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7680..7696
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.98"
                     /tool="antismash"
     misc_feature    complement(7708..7723)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.25"
                     /tool="antismash"
     misc_feature    complement(7711..7729)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.85"
                     /tool="antismash"
     misc_feature    7714..7727
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.47"
                     /tool="antismash"
     gene            complement(7749..8855)
                     /locus_tag="AMK28_RS17190"
                     /old_locus_tag="AMK28_16975"
     CDS             complement(7749..8855)
                     /NRPS_PKS="Domain: Aminotran_5 (16-293). E-value: 2e-28.
                     Score: 91.3. Matches aSDomain:
                     nrpspksdomains_AMK28_RS17190_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="AMK28_RS17190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16975"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073742087.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-22, bitscore:
                     76.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADHFAAIERRVARLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLTVPFHAAVTADQVAEALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAP
                     AQLEMLGLEACVARIEDEGLDTVTARHASAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRVPAGTDAAALVTAALAADPALPLVAGGGALSKEMIRVNHYGADATRGAVLSSLAA
                     LGAVLTDAGRRVDIEAARRAVSETWPSL"
     aSDomain        complement(7977..8807)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMK28_RS17190_Aminotran_5.1"
                     /evalue="2.00E-28"
                     /label="AMK28_RS17190_Aminotran_5.1"
                     /locus_tag="AMK28_RS17190"
                     /protein_end="293"
                     /protein_start="16"
                     /score="91.3"
                     /tool="antismash"
                     /translation="AAIERRVARLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNV
                     VTGPYGQTFGNWLRDCGAEVVDLTVPFHAAVTADQVAEALAEHPEIDFVSLVHAEAATG
                     NTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAV
                     SVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAPAQLEMLGLEACVARIE
                     DEGLDTVTARHASAAAATRAGAVALGGGLEPYVHEARDAAPVATTLRVPAGTDAA"
     gene            8987..10087
                     /locus_tag="AMK28_RS17195"
                     /old_locus_tag="AMK28_16980"
     CDS             8987..10087
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="AMK28_RS17195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_16980"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_079199020.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.2e-18, bitscore:
                     63.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEEQRVLRVKGRVLVGPDEVRDELWAVGGRITYERPPGADRA
                     VTVRGWVLPGLVDAHCHVGLDRHGPVDAATAEKQALTDREAGTLLIRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEESLADLVEAGIDCVEHATGLTEDTI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEARFPRWSAHMRQLYDRRYDTVRAAYDAGIPV
                     YVGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATAWGARRWLGRPALEEGAPADLVV
                     YDEDPRADVRVLAAPRHIVLNGRVIG"
ORIGIN
        1 gaaggaccgt cccgtcaccg ctctcaccac cgtcggcggg gatgcggcgg cctgcgaccc
       61 ggacgggtcc tgcgaggttc cgcggagctg agcccgcccg agaactcatc tataagcgtt
      121 cctttgatgc tcccttgact ctccgtgatt gactttgggt caagggagct tcagggtgga
      181 cgtatgacga tccttgcgcc catccaagcg gcagccgtgg aattcgcgcc cgaggtcacc
      241 tacctcaaca cctccagctg gggcctgctg ccccgccgga cgatcgccgc cgtcaaggcc
      301 ctggccgacg agaacgccgc cgggcgccgg gtgggggcgg gcagcttcga cgcggtggag
      361 gccgcccggg ccggcttcgc ccggctcgcc ggggtccgtc cggaccgggt cgccaccggc
      421 agttccgtca ccgtccacgt cggtctgatc gccgcctcac tgccgccggg cgccgaagtc
      481 ctctgccccg agggtgagtt ctcctccgtg gtcagcccgt tcgcgctccg cggtgatctg
      541 cgcatgcggt acgcgccgct cgacggcctc gccgcggccg tgcgcccctc gacctctctg
      601 gtcgccttct ccgccgtaca gtcggccgac ggccgggtgg ccgatctcgc cgccgtccgc
      661 gcggccgccg cggcgcacgg ggcccggacc ctgctggacg gcacccaggc ggcgggctgg
      721 ttcccgctgg aggcgggggc gtacgactac accgtcaccg gcggcttcaa gttcctgctc
      781 tgtccgcgcg gtacgtcgtt cctgaccgtc accgaggagg cgcaggacac cctgccgccc
      841 ctcttcgccg gctgggtctc cgccggtgct ccctggacga gcaactacgg cccgctggag
      901 cgcctcgccc ccaccgcccg cggcttcgac gagccgcccg ccttcctctc gtaccacggg
      961 gccgaacact ccctggacct gctcgccgag gtgggtgccg acgccctgta cgcgcacgcc
     1021 accgggctcg cggcccggct gcgggcgggg ctggcgaagc tcgggcacgc gacggttccc
     1081 ggggagtcgg cgatcgtctc cgtgccgggc ctggagagcc gtcagtccga cctggtggag
     1141 gccggtgtcg ccgtctcggc cccggccggg aatctgcgga tctcctgcca cctctacaac
     1201 accgaggccg atgtggaccg ggtgctggag gtcctggcct gatccgtgcg ggggcggttc
     1261 agcaccggtc cgggcagtcc gcggtctccc ggcagaggtt ctcctcgacc ttcgccagca
     1321 gccggaccaa ctccgcgcgc tcggccgggt cgaggcccgc gagcgtgtgc tcctccagac
     1381 tgccccaggc gtcccggacc gccgtgtgca gcgcgcagct ctcgtccgtc gcctcgacca
     1441 gcacggcccg ccggtcggcc gggtcggggc ggcggcgcac atgcccggtc tgctccagcc
     1501 gctggagcat cttcgtgacc gtggacgggt ccaggtccag ggccctgatc agctccgtct
     1561 gacggaccgc gccccggtcc cacagatgca tcatcagcaa ctcctgcccc gggtagaggt
     1621 ccaccccctt gagcgccttg cccgccgccg tccggtgcag ccgggccacc cgggacacgg
     1681 cgtggctgac gggcccgctg agcgccgcca tgggcagcgc gttcgtacag gcgtcttccg
     1741 cgggggtggc gcggccggtg gtcatggcgg gctcctgggg gtcctcgggt tacaggcggc
     1801 ggctctcgat gatgcccgac ggtttcccgc aactttacct tggtcgacca atcaatgggt
     1861 tacagttgcc gctgtcgccg atgcttggcc gaccacatac tttcttggga gccttcatga
     1921 ccaccgcttt cgacccgatc gacctgtccg gcgcccagct ggccaaccgc atcgcgctgg
     1981 ccccgatgac ccgcagccgg gccggtgagg gcggcaccgc caccgagctc accgccacgt
     2041 actacgccca gcgcgcctcg gccggcctcg tgatcaccga aggcatccag ccgtccgtcg
     2101 tgggccaggg ctaccccacc acgccgggtc tgcacagcgc cgaacaggtc gcctcctggc
     2161 gcaaggtcac cgacgcggtg cacgccgagg gcggccggat cttcgcccag atcatgcacg
     2221 cgggccgcat cggccacccg gtcctgctcc ccgacggcct cgtccccgtc agcgcctccc
     2281 cggtcaaggc cgaggggcag gtctacaccc acgtcgggcc gaaggacttc gtcgagccgc
     2341 gcgagctgac ggacgccgag atccacgcga ccgtcgccga cttcaccgcc gcctcccgca
     2401 acgccgtgga agccggattc gacggggtcg agctgcacgg cgccaacggc tatctcatcc
     2461 agcagttcct ggcccccaac accaaccggc gcaccgacgc gtggggcggc tccgacgagg
     2521 cccgcatccg cttcgccgtc gaggtcgtca aggccgtcgc cgccgagatc ggcccggagc
     2581 gcaccgcact gcgcatctcg cccggcaacc cgtacaacga catcgacgag cccgctccgg
     2641 aggccacgta caccgcgctg gtgcaggcga tcgagccgct cggcatcgcc tacctgcaca
     2701 tcctggaggc cgccccgatc cgggagctga ccctcgccct gcgcaaggcc ttctccggga
     2761 ccgtcgtcct caacgtgcac tccgacggcg ccaccggacc cggtgaccac acggtgatcg
     2821 acgacggcat cgccgacctg atctcctacg gggcgctctt cctcgccaac ccggacctgc
     2881 ccgcccggct gagggcgggc ggcccgttca atacccccga cccgtcgacc ttcttcggcg
     2941 gcgacgccaa gggctacacc gactacccgg cgctcgactc cgtctgagcg tcctcgcgcg
     3001 gcctcccgtc ccaccgccac gaggtacggc gcgggtggtc cggcagctcg gcgcggccgg
     3061 tggcccacag caggacggtc caccggtcgc cgcccgccgg ggcgtccggg aagagccggg
     3121 ccagcacccg gtcgcagagc gccggggacg gcgaccagtc cagccccagg ccctcggcga
     3181 ggtcgtacgt gtgcaccaga gtctccacca cgcccatcgc ggcgaagccc tccgggtcgg
     3241 agatcccgta cccgtggtac gagcgcacct cgggcggcgt cgtccgcgcc atcgaggcca
     3301 gcagcgcccc gctcgcctcc agcgtggcca gcagcccggc gggaccggcg tcacggtcgg
     3361 cgaagaccgc gttgtagggc ccgccctgcc gttcgggcgc ccaccggtag ggcacgtccc
     3421 ggtccagcgg aggcgtacgg ggtcccaact gcacggcgta cgcgaacaga tcgtcgctca
     3481 ggtgctccac cgtctcccag caggtccagt cgaggcttcc cgcgggcgcg tcccagcggt
     3541 ccgccggggc tccggccagc acgtccaccg cgagccgcac cgccgaggtc acatcgtcgg
     3601 cggtcaccgg cagccgggtc ccggtgagcg ccgcccgctc ggcggcggag cactccacac
     3661 agaactcgtt gccctcggga tcggcaagcg tcgcccagcc gcgcccgttc ggcctgcggt
     3721 ggtcgcccac cagggtcgcg ccgagcgcga gcagccgctc cacctcctcg tcccgggagc
     3781 ggtcctgcgg ctggatgtcc agatggacgc ggttcttggt ctgcttcttc tcgtccaccc
     3841 ggacgaagag gatcgccgcg ccgggagcct cgaccagagc ctccgggtct ccggggaagt
     3901 cctcgtcgga gagcggcgcg ccgagcacct gggcccagaa gcagccgagc gcgtaggcgt
     3961 cggcgcagtc gatcgtgatg tgacgtacat gggaagtcat ggcggacacc ctctccgctg
     4021 acccggcccc ggtccagcga gttacccggg aaaggccggg acggcatacc cgtcccggcc
     4081 cttcccgccc gtgcggcgcg ctacttcacc ggggtgaagt cccgcgcccc gatgaactcg
     4141 gggcggcgga tcggagccgc gaagggctcc tgcgccgcgt tctccacgct gttgaagacg
     4201 atgaagacgt tgctgcgcgc gtacggggtg atgttgtcgc ccgagccgtg catggcgttg
     4261 cagtcgaacc aggtcgccga accggccctg cccgtgaaga gcctgatgcc gtggcggtcg
     4321 gccatcttcg tcagcgcctc gtcggacggg gtgccggcgt cctgcatctg gagcgacttc
     4381 ttgtagttgt ccttcggcgt ctcgcccgca cagccgagga acgtcttgtg cgaaccgggc
     4441 atgatcatca gcccgccgtt ggtgtcgaag ttctcggtca gcgcgatcga cacggacacc
     4501 gtccgcatgt tcggcagacc gtcctccgca tgccaggtct cgaagtcgga gtgccagtag
     4561 aagcccgtgg ccccgaagcc cggcttcacg ttgatccggg actggtggac gtacacgtcc
     4621 gagccgagga tctggcgggc ccggcccacc acccgctcgt cgcggaccag accggcgaag
     4681 acctcgctga tccggtggac ctcgaagacg gaccgtacgt tctgcgactg cttctcgacg
     4741 atcgagcgct cgtcggcgcg gaccgcaggg tcggagatca gccggttcag ctccgcctgg
     4801 tagacggcga cctcgtccgg ggcgatgagc tggtcgacgg tgaggaagcc gtcgtgctcg
     4861 tatccctgga ggtccttggc ggcgaccgga cccggtgcgc ccggcgcgga ccagatgacc
     4921 gggtcctggc ggggagtggt catctcggcg gcgccgcgcg aggggtacag atcggcgcgt
     4981 acttcggtgg tcatggtggt tcaggcctcc tctgtcagca gtgggtagac accgttctcg
     5041 tcatggtcct cccgtcccgt gacggggggg ttgaagacac acacgcagcg gaagtcggtc
     5101 ttgggccgca tggtgtgccg ctcatgcccg tccagcaggt acatggtgcc cggcgtgatc
     5161 cagtgcgtct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagcacc
     5221 gcctcgatgt ggttcgcgta ccacatggag gtctccgtgc ccgcgtacag cacggtctcg
     5281 tggagcgaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gatgccgcct tcacatgccg gtcggtgttc tcgatgtcac tgaacgatcg gacgatcacg
     5401 gtgagcggtt gcctttctct ctgtacagcg tcccgggcct cgcggcccgg gacctctctt
     5461 ctccgcctgc cccggcagtg ccgggtcagg ccgtttcgcg gacgcagcgg gccagcgtgc
     5521 gcaggccctc gtccagctct tcgggggtga tggtcagcgg cggcagcagc ttgacgacct
     5581 cgctctgcgg gcccgaggtc tccaggagga gccccagctg gaaggcgcgg gcgcagaccg
     5641 ccgaggcgcg cgccttgtcc tcgaactcca tgccccacac caggccccgg ccgcggaact
     5701 gcgcggtcgg ctcctcggcg cagatggcca gcagggtctg ctcgacctgt tcgccgcggg
     5761 cgagggtctg cttctccatc tggccgtcgg cccagtaggc gtccagggcg gcggcggccg
     5821 tgacgaaggc cgggttgttg ccgcggaagg tgccgttgtg ctcgccgggc tcccagatgt
     5881 ccagctcggg cttgaagagg cagagggaca tgggcaggcc gtagccgctg atggacttgg
     5941 agagcgtgac gatgtccggg acgatgccgg cctcctcgaa ggagaagaag ccgccggtgc
     6001 ggccgcagcc catctggatg tcgtcgacga tcagcagcat gtcctggcgg tggcacagct
     6061 cctggagcgc gcgcagccac tcggcgcggg cgacgttgat gccgccctcg ccctggacgg
     6121 tctccacgat cacggctgcg ggcttgttga gcccggagcc ctggtcctcg aggagacgct
     6181 cgaaccagag gaagtcgggg accgtgccgt cgaagtagtt gtcgaacggc atcggggtgc
     6241 cgtgcaccag cgggataccg gccccggccc gcttgaacgc gttcccggtc accgcgagcg
     6301 agcccagcga catgccgtgg aaggcgttgg tgaacgagac gaccgactcg cggcccttga
     6361 ccttacgggc cagcttcagc gccgactcga cggcgttggt gccggtcggg ccggggaaca
     6421 tgaccttgta cggcagatcg cgcggccgca ggatcacgtt ctggaacgtc tccaggaacg
     6481 cccgcttggc ggtggtcgcc atgtccaggc cgtgggtgat gccgtcgcgc tcgatgtagt
     6541 cgatcagcgc tcgtttcagc accgggttgt tgtggccgta gttgagcgag ccggccccgg
     6601 cgaagaagtc caggtaggag tggccgtcct cgtcggtcag ccgggcgccc tgcgagcggt
     6661 cgaacacggc gggccagccg cggcagtagc tccgtacctc ggactccagg gtctcgaaga
     6721 cgctcagggc gggcggggtg atggtcacag cggatctcct gcgtgagggg ggggtgtgac
     6781 gggcagtggt cgagtgctcc cggccgcacg ggtgcggccg ggaccgcgcc gggtggtcgg
     6841 acggcgcgag aagtcagggg gatccggcgt acgggtcggg tacgccgggg tgcggtggcc
     6901 ggggcggctc aggtggcgaa cgggcctatc cggtagagca cctcgggcag gtgcgtctct
     6961 tcggggaaca gctcgccgtc gaagagcact tccttctcca gtgcgacgtc gtggcgctgg
     7021 gcgtaggacg tgaacagccg gtcggaggcg gtgttgtccg gcgtgaccgt cgtctccacg
     7081 gacgacaggc cctggtcggc ggcgacccgg gcggtcagcg agtccagcag agcggcggcc
     7141 agccccttgc cgcggtgggc ctggtcgacg gccacctgcc agacgacgag ggtctggggg
     7201 cggtcgggcc tgatgtagcc cgtcacgaag gcgatgggct cgccgttctc gccacgggcg
     7261 acggcggagg tcgcggcgaa gtcacggcac cacagcaggt agctgtacga ggagttcagg
     7321 tccaggacct gggagtcgcg ggctatgcgc cagatcgcgg ctccgtcctc cactcgtggc
     7381 gcatcgatac tgaggaattc gcttcgggca cgggcaaaat ctgctggtgc ggcggtcatg
     7441 cgaattgaat ttacccagca aatttcgaaa ttgcatcgac gtagagggtt gggtggaggg
     7501 ctcctttgtg ttatcaggcg tgcgcgaggt tcccggtgat tcaggtgcat tttcctgcac
     7561 tttgtcctga atttttcggg cataatgccg accctgtgga gtcggtcaca gggttgtaac
     7621 tctcgcgaga tgtgtccgaa tcatgaagtt cgcggccaga aaaaactggc ccgtttaggt
     7681 gacgggaaag cgggcagaag aatgtgggaa gctgcttttc aataaagcag ctttccgcat
     7741 gaggaatatc agaggctcgg ccaggtttcc gaaacagccc tacgggcagc ctcgatatcg
     7801 acccgccggc cggcatcggt cagcaccgcc cccagagccg cgagcgagga cagcaccgcg
     7861 ccccgcgtcg catccgcccc gtaatggttg acccggatca tctccttgga cagcgccccg
     7921 ccgcccgcga cgagcggcag cgcggggtcc gccgccagcg ccgccgtgac cagcgcggcc
     7981 gcgtccgtcc ccgccgggac gcgcagcgtc gtcgccaccg gggccgcgtc ccgcgcctcg
     8041 tggacgtacg gctccagccc gccgcccagg gccaccgcac cggcccgggt ggccgcggcg
     8101 gccgaggcgt ggcgggccgt caccgtgtcc aggccctcgt cctcgatccg cgccacgcag
     8161 gcctccaggc ccagcatctc caactgcgcc ggggcgtgcg gcagcgccgt acggccgccg
     8221 tcgatccagc gctccttcca gtccaggagg gagaggtagg agcggcgcgg ggcggccggg
     8281 ttggcggcga tccgctccca ggcccgcgcg ctcaccgaca ccgccgacac cccggcgggc
     8341 ccgcccatgg ccttctgcgc gccgatcacg cacaggtcga cgccccaggc gtccggcagc
     8401 agtggctcgg cgcccaccga ggcgaccgcg tccagcatga acagcgcgcc gtgcgcccgg
     8461 accacctcgc cgatctccgc gaccgggttg gtgttgccgg tcgccgcctc cgcgtgcacc
     8521 agcgagacga agtcgatctc cgggtgctcg gcgagcgcct cggcgacctg gtcggcggtg
     8581 accgcggcgt ggaagggcac cgtgaggtcg acgacctccg cgccgcagtc ccgcagccag
     8641 ttgccgaagg tctgcccgta cggtccggtg accacgttca gcgcggtcga gccgggccgt
     8701 gcgccgccgc ggatgcagcc ctccagcggc agcagcgcct cgccctgggt gatgacgacg
     8761 tcctgctccg tggcgaggag ccgggccacg cgccgctcga tcgccgcgaa gtggtcggcg
     8821 gtgagcgggg ggaggtcgag gaaggggtgc gtcacggtgg ggctctcttc ggggtcgttc
     8881 gcgggtccat gggcaggcgc cggtgaaacg ttcccgcctt ccgggcgggc ggcgggacat
     8941 tccacaggcc ccagcagcct accgacggcc tcgtacagtg ccgcgtatga gtgatcagga
     9001 ggagcagcgg gtgctgcggg tgaagggccg ggtgctcgtc ggcccggacg aggtgcggga
     9061 cgaactgtgg gccgtgggcg ggcggatcac ctacgagcga ccgcccggcg ccgaccgcgc
     9121 ggtgacggtc agggggtggg tgctgcccgg actggtcgac gcgcactgcc atgtgggcct
     9181 ggaccggcac ggccccgtcg acgcggcgac cgccgagaag caggcgctca ccgaccggga
     9241 ggccggaacg ctgttgatcc gggacgcggg atcgccctcc gacacccggt ggatcgacga
     9301 ccgcgaggac cttccgaaga tcatcagggc cggccgccac atcgccagga cccgccgcta
     9361 catccgcaac tacgcccatg agatcgaacc cggcgacctc gtcgcgtacg tggcccggga
     9421 ggcccggcgc ggggacggct gggtcaagct ggtgggagac tggatcgacc gcgacgccgg
     9481 ggacctgacc gcctgctggc cgcgcggcga ggtcgaggcg gccatcgccg aggcgcaccg
     9541 gctcggcgcc cgcgtcaccg cgcactgctt cgccgaggag tcgctcgccg acctggtgga
     9601 ggcgggcatc gactgtgtcg agcacgccac cggcctcacc gaggacacca tcccgctctt
     9661 cgccgagcgg ggggtggcga tcgtcccgac gctggtcaac atcgccacgt tccccgatct
     9721 cgcggcgggc ggcgaggcca ggttcccgcg ctggtcggcc catatgcggc agctgtacga
     9781 ccgccgctac gacaccgtgc gcgccgcgta cgacgccggg atccccgtct acgtcggcac
     9841 cgacgcgggc ggctccctgg cgcacgggct ggtcgcgggc gaggtcgccg aactcgtcaa
     9901 ggcgggcatc ccgcccctgg aggccctctc cgccaccgcg tggggggcca ggcgctggct
     9961 ggggcggccc gcgctggagg agggcgctcc ggcggacctg gtcgtgtacg acgaggaccc
    10021 gcgcgcggac gtccgggtgc tggcggcgcc tcgtcacatc gtgctgaacg ggcgggtgat
    10081 cggctgagag cggcgcggtg cggtgttgac gagcagtgac ggatacggtc ccggactcgt
    10141 tgatgcacct ctgcgcacgc tccggcgcga gcgcgctccg cacggctcgc gccgggtgaa
    10201 acgagtgaca tcagcgaacg cacgtgccga aagattcgaa tatgcgcgcg gaaacccccc
    10261 tttggggtga actcacgcca ggtatccgcc agttcaccct cagtgcgtaa agattcacgg
    10321 agtcgaggcc gtcggcgcag gcgatgtccc ccattggact gtgccggcgg ctccatgtct
    10381 cttgtggggg ttccaccat
//
