LOCUS       NZ_LIVW01000001        10423 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02115 scaffold1, whole genome shotgun sequence.
ACCESSION   NZ_LIVW01000001
VERSION     NZ_LIVW01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02115
  ORGANISM  Streptomyces sp. CB02115
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2387836)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387836)
  AUTHORS   Yan,X., Ge,H., Hindra,F., Yang,D., Huang,T., Crnovcic,I. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905645.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,651
            CDSs (total)                      :: 7,572
            Genes (coding)                    :: 7,475
            CDSs (with protein)               :: 7,475
            Genes (RNA)                       :: 79
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 34 of 97
            Pseudo Genes (incomplete)         :: 79 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 777053
            Orig. end                         :: 787476
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LIVW01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="4"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5423
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            94..870
                     /locus_tag="AMK28_RS03410"
                     /old_locus_tag="AMK28_03355"
     CDS             94..870
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950999.1"
                     /locus_tag="AMK28_RS03410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03355"
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="WP_073740139.1"
                     /transl_table=11
                     /translation="MRSRPVGFLLSALAGAALAFGGTAAASATPPSAPTSAPTGNVGTA
                     DACYTWSGTLQEGSSGEAVRQLQIRVAGYPGTGNQIAIDGAFGPATKAAVARFQSAYGL
                     AADGIAGAQTFSKIYALQDDDCTPVNFTYAELNRCNSDWSGGKVSAATARANALVTMWK
                     LQAMRHAMGDRPITVNGGFRSVSCNSAVGGAANSRHMYGHAADLGAGSQGFCGLAQAAR
                     NHGFTEILGPGYPGHNDHTHVAGGSGRFWSAPSCGI"
     gene            complement(944..1375)
                     /locus_tag="AMK28_RS03415"
                     /old_locus_tag="AMK28_03360"
     CDS             complement(944..1375)
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966557.1"
                     /locus_tag="AMK28_RS03415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03360"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_073740140.1"
                     /transl_table=11
                     /translation="MEAHASELALGQKVLEAQPFSVLLGARLTAFGNGAAVLELDVRDE
                     LRQQNGFVHGGVLSYAADNALTFAAGAAVGPSVLTAGFTIDYLRPARGEVLRARAKVVR
                     PGRTRVVCRCDLLMVDAAGVETLCAVAQGTIAVAGDSGS"
     gene            1481..2500
                     /locus_tag="AMK28_RS03420"
                     /old_locus_tag="AMK28_03365"
     CDS             1481..2500
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379201.1"
                     /locus_tag="AMK28_RS03420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03365"
                     /product="hypothetical protein"
                     /protein_id="WP_073740141.1"
                     /transl_table=11
                     /translation="MENMIPRGNWLDDDIFREQVREVTPLRFGSWSLPEFERATSELGW
                     ELREPKEVVGRIWRRFAPRKGPRSGYGTVISDASAPDQVRKLNIRAVDLPAEDIATAAG
                     LVRAAWWVMEEELGPPTMWGGDSGPWMLWRRPGVCLLVHSHDEGEVSLELLPPDADADC
                     AGSGYSRGRWRAAELAGLPSAPAAPSAPGTTWEEIEKRLSEALRSLCYDTPFFPGRFIL
                     HLGSADDAQRFVQCWIQDLGLVVEATGYLHRPEAVDAARLTDNGWDASRSVWQRRFPDA
                     MDDPAHAATAARMLVGELRHLGVGLADLSYDGTMTGRGRGFHLELPDLGIPRVHRPAV"
     misc_feature    complement(2708..2722)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 28.15"
                     /tool="antismash"
     misc_feature    2709..2723
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 28.65"
                     /tool="antismash"
     gene            2739..3716
                     /locus_tag="AMK28_RS03425"
                     /old_locus_tag="AMK28_03370"
     CDS             2739..3716
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379202.1"
                     /locus_tag="AMK28_RS03425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03370"
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="WP_073740142.1"
                     /transl_table=11
                     /translation="MRRLLRTLAASAFACLLLPLGAGQQAAATAQADPIGMTSGFYTDP
                     GSTPAAWVAANPGDGRAAAIRDNISSRPMARWFGSWSGDIGAAVGSYVGAADAADKLPV
                     LIAYNIPGRDACGGHSGGGAGTPGAYHAWISAFASAIGTRPALVVIEPDSLGDFSCLSR
                     TQIDERNGMLRDALAQFAARAPHTWTYLDAGNPAWIGAATMAQHLDGAGARQAHGFSLN
                     ISNYYGTGENSAYGNAVNGSLSASYGYTKPYVIDTSRNGNGSDGAWCNPGGRRTGSVAQ
                     TGGGAEMLLWLKTPGESDGDCGVGAGSVAGQFLPEVAYKMIYGY"
     gene            complement(3754..4422)
                     /locus_tag="AMK28_RS03430"
                     /old_locus_tag="AMK28_03375"
     CDS             complement(3754..4422)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455530.1"
                     /locus_tag="AMK28_RS03430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03375"
                     /product="DUF4232 domain-containing protein"
                     /protein_id="WP_073740143.1"
                     /transl_table=11
                     /translation="MFGKRRKAFLVSAALVGGALLMTACQDTEDGAAPSASATDVPAAD
                     AAGADASKGADKGAEQGAGQEPDAKDGGNAEGSGADAGDGTKVPVQQACGANDISWSTR
                     SESQAGGYILIMAKAKQGITCILPAALPTVAFGSDGTEAGPAEQSVGDAVTLSGATTAY
                     AGVNPKTTDTDGGKELDSIIVGVGNEDPDPVSLEVGTITVDKPVVTNWHTAPADAVPSA
                     "
     misc_feature    4416..4429
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(4416..4430)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.49"
                     /tool="antismash"
     gene            5001..5423
                     /locus_tag="AMK28_RS03435"
                     /old_locus_tag="AMK28_03380"
     CDS             5001..5423
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966560.1"
                     /locus_tag="AMK28_RS03435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03380"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_073740144.1"
                     /sec_met_domain="melC (E-value: 2.6e-39, bitscore: 131.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRPTRRQALGAVIGVAASGALVGMAAASTETAAAEAASSGVAPG
                     GTGAPGSFDEVFEGRRIQGTPAGARTDAHAHAGHAGHAGYRVVIDGRELPVMQHGKSGW
                     SSTINHYERFATPLEAARTAVISLKGAVVVPFDPTA"
     gene            5440..6264
                     /locus_tag="AMK28_RS03440"
                     /old_locus_tag="AMK28_03385"
     CDS             5440..6264
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379204.1"
                     /locus_tag="AMK28_RS03440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03385"
                     /product="tyrosinase family protein"
                     /protein_id="WP_056700836.1"
                     /transl_table=11
                     /translation="MTVRKNQANLTAPEKKAFVDAVLEVKRLGVYDEFVLAHRTRFSID
                     MNTGVYVGHFGPSFLPWHRKFLIDFENELQKIDASVSIPYWDWTADNTVGSSLWAPDFL
                     GGTGRPLDDQVMDGPFAHSAGKWTINVSVDSRAFLARQLAYRVPSLPPRSDVDAALAMP
                     TYDSAPFRDGSTGFRSALEGSAGYMSMHNRVHTWVWGHMETSVSPNDPVFWLHHAFVDK
                     LWADWHALHPTSAEYLPAGGTTDVIDLNDTMPPWPNTTPQDMLDHTLFYTYA"
     gene            complement(6272..7780)
                     /locus_tag="AMK28_RS03445"
                     /old_locus_tag="AMK28_03390"
     CDS             complement(6272..7780)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455543.1"
                     /locus_tag="AMK28_RS03445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03390"
                     /product="DUF6056 family protein"
                     /protein_id="WP_073740145.1"
                     /transl_table=11
                     /translation="MSDDVPVTAAPEGLRGEERADPAGRPADRPWTALWVSALALPPLA
                     LLAAASWFGRWVRPGADDWCFLPAVRDDGVTGLVGTFYLDDNGRVANALLVGAYAKFQV
                     AGHQWYPLVSGVLVLAVLWAVAVLALRRARLRTPRGLPLLLAALTTALFLFVTPNTYKT
                     YLWPAASVSHTLPPVLACAALIPFLLARSRRGRAVALAVAALVGAFLATLSEETAIVVV
                     VVLLTALLLSGRVVAGPDRGFVRRWCAAGIAGTAAGAVVLITSPGSMRRRERFGAETTS
                     LLAPDSLIASLRAFAEIAVTVATTWQYAGAVAAGVLLGLLCRRADGTPPRPPAHWPLLT
                     AAGVLALLVSGYLCTVVAYPVFRDRVSDPSANRLWNDYLLLYVILLVGAGALLGLGVRR
                     LTSRTAPAKAVCAALCVLVCVGPAVSLTDLDTAMRARAEKWDAQDRRLREGAESGRRVM
                     PYERLVISDMLEPFSEGGRRYWPGGCVADFYGLDRVTDGARSAP"
     gene            complement(7790..9328)
                     /locus_tag="AMK28_RS03450"
                     /old_locus_tag="AMK28_03395"
     CDS             complement(7790..9328)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455545.1"
                     /locus_tag="AMK28_RS03450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AMK28_03395"
                     /product="glycosyltransferase"
                     /protein_id="WP_073741096.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 5.7e-28,
                     bitscore: 94.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLQLSVVVPCFDEAEVIEAFHTALVGVLDPLGDSFEICYVDDG
                     SRDRTRLLLSALAARDPRAHYTAFSRNFGKEAAMLAGLRMSRGAAVVIMDADLQHPPEL
                     IPRMLELHRHGYDQVIPRRDRAGEGLLRSTLSHTYYALVRRCMDVELIDGSGDFRLLSR
                     RAVESVLSLPESNRFSKGIFSWIGFDTVTFRYRNSERVAGRSKWGSKRLLNYGIDGLLS
                     FNNRPLRLAIYTGFWVFVSALAYALWTVVGVVLHGTDTPGYATLLTAVVALSGIQLVTL
                     GVIGEYVGRIYHEAKHRPPYVVRETDETCGALPDGPPPRPPLTGGPGERAAAPALATAA
                     APAAGTASTAPAPVTGAPGRSRTVRQFGSFVLIGCVNTAVYLGIYASLNRWIPYLTAHV
                     IGYAVSIVCSFLLNSYVTCRTRPTWHGFVRYPLSSLVNLVASGALLYGAVSGLGMDKNV
                     AALAAGVLVTPVSFLLARWAITSGRARTDAVPQAEPVPGRPAPAPLPTRSSQDR"
ORIGIN
        1 ggcagcaagt tgacatgacc ttgacacatt cggctctgcg cccgaggctc ggaacccccc
       61 gtggacagct tgacgacaat gaggtgcagc cccatgcgat cacgccccgt cggattcctc
      121 ctctctgccc tcgcgggcgc ggccctcgcg ttcggcggca ccgcagccgc ctccgcgacc
      181 ccgccctcgg cccctacttc cgccccgacc gggaacgtcg gcacggccga cgcctgctac
      241 acctggagcg gcacgctcca ggagggctcc agcggtgagg cggtacgtca gctccagatc
      301 cgcgtcgcgg gctacccggg caccggcaac cagatcgcca tcgacggggc gttcggcccg
      361 gccaccaagg cggccgtcgc gcgcttccag tctgcgtacg gcctggccgc cgacggcatc
      421 gccggcgccc agacgttcag caagatctac gcgttgcagg acgacgactg caccccggtc
      481 aacttcacct acgccgagct gaaccgctgc aactccgact ggtccggcgg caaggtcagc
      541 gccgccaccg cgcgcgccaa cgccctggtg accatgtgga agctccaggc catgcggcac
      601 gcgatggggg accggcccat caccgtcaac ggcggcttcc gcagcgtgtc ctgcaacagc
      661 gcggtcggcg gagccgccaa cagccgccac atgtacgggc acgcggcgga cctcggcgcg
      721 ggctcccagg gcttctgcgg cctcgcccag gcggcacgca accacggctt cacggagatc
      781 ctcggcccgg gctaccccgg ccacaacgac cacacccacg tcgccggggg cagtggccgc
      841 ttctggtccg cgcccagctg cggcatctga ggcacggcgt ggtggggagg cgtcgcccgg
      901 cgggccgcgc ctccccaccc gctcgcaggg gcgagtgcgg cggtcagctt ccggagtccc
      961 cggcgacggc gatcgtgccc tgggccaccg cgcagagggt ttccacccct gcggcgtcca
     1021 ccatcagcag atcgcagcgg cagacgaccc gggtacggcc ggggcggacc accttcgccc
     1081 gcgcgcgcag cacctcgccc cgggccgggc gcagatagtc gatggtgaaa ccggccgtca
     1141 ggacggacgg gccgacggcc gcgcccgccg cgaaggtcag ggcgttgtcg gccgcgtagc
     1201 tcaggacgcc tccgtggacg aagccgttct gctggcgcag ctcgtcccgt acgtccagtt
     1261 ccagaacggc cgctccattg ccgaaggccg tcagccgtgc gcccagcagg acgctgaagg
     1321 gttgggcctc caacaccttc tggccgagcg cgagttccga ggcatgtgcc tccatgctgc
     1381 acctctctct cctggtgggg tgagcgtcgc ctcccgggcc tgttccgtca acatcggccc
     1441 tgcggaaccc tgtcggcacc ggaccactac tgtctgccgg atggagaaca tgatcccccg
     1501 gggcaactgg ctcgacgacg acatcttccg cgagcaggtg cgtgaggtca ctccactccg
     1561 cttcggctcg tggagcctgc cggagttcga gcgggccacc tcggaactgg gctgggaact
     1621 ccgggagccc aaggaggtcg tgggccggat ctggcgcagg ttcgctcccc ggaaggggcc
     1681 acggagcgga tacggcacgg tgatctccga cgcctcggca cccgatcagg tgcgcaagct
     1741 gaacatccgc gccgtcgacc tgcccgcaga ggacatagcc accgccgccg gtctcgttcg
     1801 cgccgcctgg tgggtcatgg aggaggagct cggcccgccc acgatgtggg gcggcgactc
     1861 cggcccgtgg atgctgtggc gccgccccgg cgtctgcctc ctcgtgcact cgcacgacga
     1921 gggcgaggtg agcctcgaac tgctgcctcc cgacgcggac gccgactgcg ccggaagcgg
     1981 ctactcccgt ggccgctggc gtgccgccga gctcgccggc ctgccgagcg cgcccgccgc
     2041 gccgtctgcg cccggcacca cctgggaaga gatcgagaag cgcctgtccg aagccctgcg
     2101 ctccctctgc tacgacaccc cgttcttccc cggcaggttc atcctccacc tgggatccgc
     2161 cgacgatgcc cagcggttcg tccagtgctg gatccaggat ctgggtctcg tcgtcgaagc
     2221 caccggctac ctgcaccggc cggaggccgt cgacgccgcc cggctgacgg acaacggctg
     2281 ggacgcctcc cgctcggtct ggcagcgccg cttccccgac gccatggacg accccgcgca
     2341 cgccgcgacg gccgcccgca tgctcgtcgg ggaactgcgg cacctgggcg tcggcctggc
     2401 cgacctctcc tacgacggaa ccatgaccgg ccgtggacgc ggcttccacc tcgaactgcc
     2461 ggacctggga atcccccgcg tccaccggcc cgccgtgtga gacgggcccg aagcctctcg
     2521 gtcacccatg cgtgggtggg ccgtcgccgg actcattgac accggggcgg cccgctgtca
     2581 ccgtctcgtc tgacgacggc agttgcggac cgacgcccca ccggtccgcc gtacgtactt
     2641 cccaccgccc cacgcacctc cccccatttc acgcgcctcc ctccccaccc cgtcacgccg
     2701 ccgtcgattg ggagcgctcc cacagaaggg tcgcacgcat gagaagactc ctgcgcacgc
     2761 tcgccgcatc ggcgttcgcc tgtctgctgc tccccctcgg cgccggacag caggccgccg
     2821 ccaccgcgca ggccgatccg atcggcatga ccagcgggtt ctacaccgac cccggctcga
     2881 cgcccgccgc ctgggtcgcg gcgaaccccg gtgacgggcg ggccgccgcc atccgcgaca
     2941 acatctcctc gcgccccatg gcccggtggt tcggctcctg gagcggggac atcggggcag
     3001 cggtcggctc ctacgtgggc gccgccgacg ccgccgacaa gctgcccgtc ctgatcgcct
     3061 acaacatccc cggccgcgac gcctgcggcg ggcactccgg cggcggcgcg ggcaccccgg
     3121 gcgcgtacca cgcctggatc tccgcgttcg cctcggcgat cggcacgcgg cccgcactgg
     3181 tggtcatcga gcccgactcc ctgggcgact tcagctgcct gagccggact cagatcgacg
     3241 agcgcaacgg gatgctgagg gacgcgctcg cgcagttcgc cgcccgcgcg ccccacacat
     3301 ggacctacct ggacgccggg aacccggcct ggatcggcgc ggccaccatg gcgcagcacc
     3361 tcgacggcgc gggagcccgg caggcgcacg gcttctcgct gaacatctcg aactactacg
     3421 gaaccggcga gaactccgcg tacggcaacg ccgtcaacgg gtccctgtcg gcgtcctacg
     3481 gctacaccaa gccgtatgtc atcgacacca gccgcaacgg caacggctcc gacggcgcgt
     3541 ggtgcaaccc cggcggacgc aggacgggat cggtcgccca gacgggcggc ggggccgaga
     3601 tgctgctgtg gctgaagacc cccggagagt ccgacggcga ctgtggagtc ggcgccggct
     3661 ccgtggccgg acagttcctg ccggaggtcg cgtacaagat gatctacggg tactgaggac
     3721 cggcccaccc gccccggccc ggcgcccgtt ctctcaggcg gagggaacgg cgtccgccgg
     3781 ggctgtgtgc cagttggtga cgaccggctt gtcgaccgtg atggtgccga cctccaggga
     3841 gaccgggtcg gggtcctcgt tgccgacgcc gacgatgatg gagtccagct ccttgccgcc
     3901 gtcggtgtcg gtggtcttcg ggttcacccc ggcgtaggcg gtggtggcgc cgctcagcgt
     3961 gaccgcgtcg ccgaccgact gctcggcggg gcccgcctcg gtgccgtcgg agccgaacgc
     4021 caccgtcggg agcgcggccg gcaggatgca ggtgatcccc tgcttggcct tcgccatgat
     4081 caggatgtag ccgccggcct ggctctcgga ccgggtgctc caggagatgt cgttggcacc
     4141 gcaggcctgc tggaccggca ccttcgtgcc gtcgccggcg tcggcgccgg acccctccgc
     4201 gttgcccccg tccttggcgt cgggctcctg gccggcaccc tgctccgcgc ccttgtcggc
     4261 gcccttcgag gcgtcggccc cggccgcgtc cgcggcgggc acgtcggtgg cggaggcact
     4321 cggcgcggcg ccgtcctccg tgtcctggca cgccgtcatc agcagggccc cgcccacgag
     4381 ggctgcggag acgaggaagg ccttgcgacg cttgccgaac atgagatccc cttggtggtc
     4441 gtgagcgtgg tcgtggcacc gaagcgagtc cgaccgcccg ggtgttcctt gatcactatg
     4501 gggggccgtg ggccgcaggg ggtcgcatgt gccgttccag gacagcgctg tcacagaccg
     4561 aagacatgat cacggcgtgg gcgctcggcc cgcacacaac cgtcaccctc cggacggaac
     4621 gcgtccggcc cgctccggct cccccgacga actcgggtgc gcagacgggc aggtacccct
     4681 ccccggagcg agcacccact cctacttccg gccatgcacg gatgatcgtt ccgacgggcg
     4741 acggcgcgac ggattccgaa gatccttcac aggcagggca catacacggc aagacctgcg
     4801 ccttccggcc cctccggcac cggcgcggag cgggacgcgg ggccggttgc ccctctgtgt
     4861 acgtgaagac gtgtccatat gagagccaac ccctttttcc ctgcttatgc ggacggtcac
     4921 ccccgtgtag acatgatcac ggcggcccca gccacccatc cggggggccg cctccgccat
     4981 gttttggggg tacatccgcc atgcccagac ccactcgccg tcaggcccta ggggccgtca
     5041 tcggcgtcgc cgcgagcgga gcgctcgtcg gtatggccgc cgcgtccacg gaaaccgcgg
     5101 cggccgaggc cgcaagcagc ggcgtcgcac ccggcgggac cggagcaccc ggttccttcg
     5161 acgaggtctt cgagggccgc cgcatccagg gcacgcccgc cggggcccgg accgacgcac
     5221 acgcacacgc cgggcacgcg gggcacgccg gctaccgcgt ggtgatcgac ggccgcgaac
     5281 tccccgtgat gcagcacggg aagagcggct ggagcagcac gatcaaccac tacgagcgat
     5341 tcgcgacccc gctggaggcc gctcgtaccg cggtcatctc gctcaagggc gccgtcgtcg
     5401 ttccgttcga ccccaccgcc tgacccggga gcagcagcca tgaccgtacg gaagaatcag
     5461 gcgaacctca cggcgcccga gaagaaggcc tttgtcgacg cggtactgga ggtcaaacgc
     5521 ctgggcgtgt acgacgaatt cgtcctcgcc catcgcacgc ggttctccat agacatgaac
     5581 accggcgtgt acgtgggcca cttcgggccg tcgttcctgc cctggcaccg caagttcctc
     5641 atcgatttcg agaacgaact acagaagatc gacgcgtcgg tctcgatccc ctactgggac
     5701 tggaccgccg acaacaccgt cggctcctcg ctctgggccc cggacttcct cggcggcacc
     5761 ggacgccccc tggacgacca ggtgatggac gggcccttcg cgcattccgc cggaaagtgg
     5821 acgatcaacg tctcggtcga cagccgcgcc ttcctcgccc gtcagctcgc ctatcgcgtt
     5881 ccctcgctcc cgccccggtc cgatgtcgac gccgctctcg ccatgcccac ctacgactcc
     5941 gcacccttcc gtgacggctc gaccggcttc cgctccgcgc tggagggctc cgcggggtac
     6001 atgagcatgc acaaccgggt gcacacctgg gtctggggcc acatggagac gagcgtctcc
     6061 ccgaacgatc cggtgttctg gctgcaccac gcgttcgtcg acaagctctg ggccgactgg
     6121 cacgcactcc atccgaccag tgccgagtac ctccccgccg ggggtacgac cgatgtgatc
     6181 gatctgaacg acaccatgcc gccctggccg aacacgaccc cgcaggacat gctggaccac
     6241 acgctgttct atacgtacgc ctgagtgctc gtcacggggc gctcctggcc ccgtccgtga
     6301 cccggtcgag cccgtagaag tcggcgacgc agccgccggg ccagtacctg cggcctccct
     6361 cgctgaaggg ctccagcatg tcgctgatca ccaggcgctc gtacggcatg acccgcctcc
     6421 cggactccgc cccttcgcgc agccggcggt cctgggcgtc ccacttctcg gcccgggccc
     6481 gcatcgcggt gtccaggtcg gtgagcgaca cggccgggcc gacgcagacc agcacgcaga
     6541 gcgcggcgca caccgccttc gccggggcgg tgcggctggt gaggcggcgc acgcccaggc
     6601 cgagcagggc gcccgcgccg acgagcagga tcacgtacag caggaggtag tcgttccaca
     6661 gccggttggc gctcgggtcg ctcacccggt cgcggaacac cggataggcg acgaccgtgc
     6721 agaggtagcc ggagacgagg agagcgagca ccccggcggc ggtgagcagg ggccagtgcg
     6781 cgggcggacg gggcggtgtc ccgtccgccc gtcggcacag cagccccagc agtacgcctg
     6841 cggcgaccgc gccggcgtac tgccaggtcg tggccacggt gacggcgatc tcggcgaacg
     6901 cgcgcagcga cgcgatgagg gagtcggggg cgagcaggga ggtggtctcc gcgccgaacc
     6961 gctccctccg ccgcatcgag ccgggtgagg tgatgaggac gacggccccg gccgccgtcc
     7021 cggcgatccc cgccgcgcac cagcgccgga cgaacccccg gtcggggccg gcgacgaccc
     7081 ggccggacag caggagcgcg gtcaggagca ccaccacgac gacgatcgcc gtctcctcgg
     7141 acagcgtggc gaggaaggct cccacgagcg ccgcgacggc gagcgcgacg gcccgccccc
     7201 ggcgggagcg ggcgagcagg aacgggatca gcgcggcgca ggccaggacg ggcggcagcg
     7261 tgtgcgagac ggacgcggcg ggccagagat acgtcttgta cgtgttcggg gtgacgaaca
     7321 ggaagagcgc ggtggtcagg gccgcgagca ggagcggcag cccgcgcggg gtacgcagcc
     7381 gggcccggcg cagtgccagg acggccaccg cccagaggac agcgagcacc aggaccccgc
     7441 tgaccagggg gtaccactgg tgtcccgcga cctggaactt cgcgtacgcg ccgaccagga
     7501 gcgcgttggc gacccgcccg ttgtcgtcga ggtagaacgt cccgacgagg ccggtgacgc
     7561 cgtcgtcccg tacggcgggc aggaagcacc agtcgtccgc gccgggccgc acccaccgcc
     7621 cgaaccagga ggcggcggcg agcagggcga ggggcgggag cgcgagggcg ctcacccaga
     7681 gggcggtcca ggggcggtcc gcggggcgcc cggccgggtc cgccctctcc tcgccccgca
     7741 gtccctccgg ggcggcggtc acgggaacgt cgtcggacat cgcggcccct caccggtcct
     7801 gggacgagcg ggtgggcagc ggtgcgggcg ccggacggcc gggcaccggt tcggcctgcg
     7861 gtacggcgtc ggtcctggcc cggcccgagg tgatcgccca ccgggccagc aggaacgaga
     7921 ccggggtgac gagaacgccc gccgccagcg cggcgacgtt cttgtccatc ccgagaccgc
     7981 tcaccgcccc gtagagcagc gctccggacg ccaccaggtt gaccaggctg gagaggggat
     8041 agcggacgaa cccgtgccac gtcggcctcg tccggcaggt cacgtaggag ttgagcagga
     8101 acgagcacac gatgctgacg gcgtagccga tgacgtgggc ggtcaggtag gggatccagc
     8161 ggttcagcga ggcgtagatc ccgaggtaga cggcggtgtt cacgcagccg atgaggacga
     8221 agctgccgaa ctggcggacc gtacgggacc ggccgggggc gccggtcacg ggggccgggg
     8281 cggtggaggc agtgccggcg gccggggcgg cggcggtcgc aagggccggg gcggcggcgc
     8341 gttcgcccgg gcctccggtg agaggtggtc gcggcggcgg gccgtccggc agcgccccgc
     8401 aggtctcgtc ggtctcccgc acgacatagg gcgggcggtg tttcgcctcg tggtagatgc
     8461 ggcccacgta ctcgccgatg accccgagcg tcaccagctg gatgccgctg agggcgacga
     8521 cggcggtgag gagggtggcg taaccggggg tgtccgtgcc gtgcaggacg acgcccacga
     8581 ccgtccacag ggcgtaggcg agggccgaga cgaagaccca gaagccggtg tagatcgcga
     8641 ggcggagcgg gcggttgttg aaggagagca gtccgtcgat gccgtagttc agcagccgtt
     8701 tgcttcccca tttcgaccgg cccgccaccc gctcgctgtt gcggtagcgg aaggtgaccg
     8761 tgtcgaagcc gatccaggag aagatcccct tggagaagcg gttgctctcg gggagggaca
     8821 ggacgctctc cacggcccgc ctcgacagca gccggaagtc ccccgagccg tcgatgagct
     8881 ccacgtccat gcagcggcgg acgagcgcgt agtaggtgtg gctgagggtg ctgcggagca
     8941 ggccctcgcc cgcgcggtcg cggcgcggga tgacctggtc gtagccgtgc cggtgcagtt
     9001 ccagcatgcg ggggatcagc tcgggcgggt gctggaggtc ggcgtccatg atcaccacgg
     9061 ccgcgccccg cgacatgcgg aggccggcga gcatggcggc ctccttgccg aagttgcggc
     9121 tgaaggcggt gtagtgggcc cgcgggtcgc gcgcggcgag ggcgctcagc agcagtcggg
     9181 tgcggtcccg gctgccgtcg tccacgtaac agatctcgaa gctgtccccg agcgggtcca
     9241 ggacgccgac cagggcggtg tggaaggcct cgatcacctc ggcctcgtcg aaacacggca
     9301 cgacgaccga gagctgaagg ccagtcacac gccacctccg gacttcgggc ccctcgtggg
     9361 tgccgggcct caccggcgcg gcaccgtcgg cctccggcac cgggccccgg aacacgccac
     9421 gtccgcccca tctgacgacg ggcggcggcc tccgcctctc cgcacacgcg ctggggcgcc
     9481 tcttttcccg cacccgttca tgcgaacagc ggacgcgggt cggcagaaac gccgggtcgc
     9541 gggggcgaaa aagcacacat agcctcgcaa aggtgacatc tcctcgactg ccacggatcc
     9601 ggagctccac acccggcagg gcccggcttc ccggccggtc caccgggcct ctcgccggta
     9661 cgctcaccgg cctgctcgcc gtactgtccg ccgtgtgctg gctgctgctc cccgccgccg
     9721 cgaccgcacg cgccgacgac cccgtcgagc tgtcccggga cgggcagatc accgaccggg
     9781 tgggagccct gggcgaccgg acggggcagg tggaggacgc gctcgaccgg ttgtacgacg
     9841 acgagcgcat ccagctcttc gtcgtgtacg tacgcgattt ctccggccgg tccggacaga
     9901 cctggagcga cgagaccgcc aaccggaacg gtctgggcca ggacgacgtg ctgctctccg
     9961 tcgccaccca cgaccggcag tacgcctact ccgtcgacgc cggctccccg ctcaccgacg
    10021 agcagctgcg ggacgtggcg ggcaccgcga tcgaacccgc cctgcgggag aacgactggg
    10081 cgggggccgc gatcggcgcg gccgacggct acggctccgc actggccgga cgcccggtga
    10141 ccgctcccgc cgtcaccccg gggccagccg accccggaac gggcgactcc ggctccaccg
    10201 gcgcccgcga cttcatcctg ccggcagccc tcgtggtggg cgtgggcgcg gtcggcgcct
    10261 acgcgtacgc ccggcgcaaa cggcgcacga cgacccgcac caccccggcg gccaccggtt
    10321 ggggccgggg cccgggggcg ggcggtgcgc cggagccgcc gacaccactg ccggagctgg
    10381 acgggcaggt caagcaggta ctggtggaca ccgacgacgc ggt
//
