LOCUS       NZ_LFBV01000013        11371 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces uncialis strain DCA2648 scaffold13, whole genome
            shotgun sequence.
ACCESSION   NZ_LFBV01000013
VERSION     NZ_LFBV01000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces uncialis
  ORGANISM  Streptomyces uncialis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 155014)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Cloning and characterization of the uncialamcin biosynthetic gene
            cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 155014)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33410, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-MAY-2015
            Assembly Method                   :: CLC Genomics Workbench v. 8.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905345.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,456
            CDSs (total)                      :: 7,371
            Genes (coding)                    :: 7,214
            CDSs (with protein)               :: 7,214
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 42 of 157
            Pseudo Genes (incomplete)         :: 143 of 157
            Pseudo Genes (internal stop)      :: 7 of 157
            Pseudo Genes (multiple problems)  :: 33 of 157
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 49525
            Orig. end                         :: 60896
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LFBV01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11371
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11371
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11371
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6371](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6371)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(22..1677)
                     /locus_tag="AB852_RS36540"
                     /old_locus_tag="AB852_35950"
     CDS             complement(22..1677)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512964.1"
                     /locus_tag="AB852_RS36540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_35950"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_245877068.1"
                     /transl_table=11
                     /translation="MTVRTFVAALAGQTALAALVVLAAHTVGTAVMTRAAPGTGTVLAL
                     VALVLVRAATTWWEMDLSHDLAYRVLAELRVRVFDGLARSAPLRIGGRRSGDLASAALA
                     DVEALEFFYAHAVAQLLATTAVFGAGTVVLADLEPWLLPAVLPMAAALMLGPLLEGRDR
                     AALGARTRSAAAELSARAVETVDGLPELLAFGAFDRRRADLRGYGRLLGAAQRAEQTRE
                     ARSAAVRDILVVISLVAVVAVSAHTLEGAWVPAAVALALGVLAPVADSAAALGQAACLR
                     AAAARVGAAVRAPRGAPEPRSPRPVPDGPLGFRLSGVRFGYGGGPVLDGLDLLVRPGET
                     VALVGASGAGKSTCAHLLARFWDPADGRVELLAGDGRSVDLRQVAESELRAAVAVVGQD
                     APLFHGTLRENLRLGTPAASDADLVAVAALTGVDRIADGLPQGFATMLGERGSTLSGGQ
                     RARVALARALIARPRVLVLDETTSNLDGLGDAELLAALPDCATLIIAHRAATIRRADRI
                     AVLEDGRVAQEGTWAELTAVPGPFTRVLDREQRG"
     gene            complement(1797..3521)
                     /locus_tag="AB852_RS36545"
                     /old_locus_tag="AB852_35955"
     CDS             complement(1797..3521)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993088.1"
                     /locus_tag="AB852_RS36545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_35955"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_073795742.1"
                     /transl_table=11
                     /translation="MMLHPELLRAARTARRPLLLATALLAATACTHLLQAVLLALVLGA
                     VASGDLTGLPPLLAAVLAAVAARALLTRWQRRVAVGAGSAVRVGLRDELLVRLGRTGPA
                     RHTDVRSGAVRATLVDGVEGVDAYVSRYLPQFLVTCVLPLPPLVAVTVLEPWAGAVLAP
                     ALLLALFAPRWWDRLLARRGERHWGAYEALAADYLEALQGMPALRALGAVGQVRARLAE
                     RSDTLHRATVAKLRVSLVDTGITDLAVQGGTVAAVLVACWSAAAGHPAATSTYLVLLLT
                     AECFRPVRDLSREWHSGYLGVSAADGIAALRTAPGGVPDTGHLTAGPGQGPEIRFDDVR
                     FTYPGTGRPALRGVSFTAPAGRTTAIVGPSGAGKSTLVGLLLRHHEPGGGRVLLDGAEV
                     GAHTLGSLRRQIAVVSQHTYLFHDTVAENLRLARPDATDGELRAAARAAGVHDEILALS
                     DGYATVIGERGSTLSGGQRQRLALARALLADAPVLVLDEATSAVDEHREAGIVRELVRA
                     ARGRTCLVVAHRLASVRHADRIVVLDGDGRVEAVGDHTALLAADGMYARLVEAGEAA"
     gene            complement(3518..4993)
                     /locus_tag="AB852_RS36550"
                     /old_locus_tag="AB852_35960"
     CDS             complement(3518..4993)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AB852_RS36550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AB852_35960"
                     /product="nitroreductase"
                     /protein_id="WP_143201330.1"
                     /transl_table=11
                     /translation="MPTLISGALARARSSAVPGPDLRPPAHRRAPGALVAVPAALDRLL
                     ARSVAGARLRPAASAGALHPVDVLLLMGAGSGVRPGRYLYRPAEHRLQRVAAAPVPAPP
                     GVFAVLHARAERTVAHYRHRGWPLVLLDTGHTVAALVAAGAPAFTLDLDGRVLLPRTAT
                     GRATAVGLSTGRTAKGRATAARPSTDHPSTDRPATEPGGAVLAVVRLTRDGTLDPWCVP
                     ETAGASGPAPVPPPAPVPVPAQAPVPAPAQAPVPAPIPAPARDSASPGTRSPQDDDLAE
                     AARVLARLAAAGEGTGSWTTPRAPAAPDAVLLGRRSADPDVLATAGRPSSAALALLLEA
                     AASAAPDGPRWCVAVGGPAPGLLRLEGDTLTTLATGPVLPTLAIWGAGQRWIAATGAVL
                     LAHGCPWDATPARVRREHLLAGYGAGRAQLTATALGLVSRPVGSWQQADLGARLGGPPG
                     RDWIVHALAVGTPAASEDGTARTARTTQETNTR"
     gene            complement(5001..6371)
                     /locus_tag="AB852_RS36555"
                     /old_locus_tag="AB852_35965"
     CDS             complement(5001..6371)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639444.1"
                     /locus_tag="AB852_RS36555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_35965"
                     /product="YcaO-like family protein"
                     /protein_id="WP_073795746.1"
                     /sec_met_domain="YcaO (E-value: 4.1e-45, bitscore: 147.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPLSLDQLVDPVCGIVRSVAPVPHPTGAPPRYTAMTAEVADA
                     RRFGLWPADRVSLGTTFGDPDGARTAAVAEAVERYCGNRLPPPGHPAAPRRATAAELRA
                     EGRTVYGPGDLPAYAPWQYARPGFPYRPLTDDTPALWARGDEAGTEVWAPVALTHLNWR
                     QGALRDLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALEVWWRLDGPTRGIDLASV
                     PGLERELAGGGLEISAVEMPSEFAPCVAALAYDPVRDLYAAGFACRYDPAEAVRKAVLE
                     AVHTWVFTLGTLDLDGWVFRAVDAGTLARGLYLEHRADRRYLDDCGPEFASVRDLGAHV
                     QVWLDPRTAPLARRFTHPALGTVPVHDIATGSRTRLDARLAEGGHRTVTLDLTTEDIAE
                     TELRVARVLVSGLVPNAPAAFGYLGCPRLAHAAEARGWRSRPPRGPGDFTLVPPPHM"
     gene            complement(6368..7939)
                     /locus_tag="AB852_RS36560"
                     /old_locus_tag="AB852_35970"
     CDS             complement(6368..7939)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847809.1"
                     /locus_tag="AB852_RS36560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_35970"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_073795912.1"
                     /transl_table=11
                     /translation="MSVQVPVPGAALATDVRLPAGAGPFPAVVLRTPYDRRGHRGEARA
                     WAAHGFAAVVQDVRGRHASGGDWHPYRHEAGDGADTLRWIRAQPWSDGRVIAVGASYAA
                     YCALVTAVPGAEPAPPATTGAPAPAASVPRSAAAPDGVIAAVPAVGQAAVAREPSGVER
                     LLGRAGWWAAHGDRAGSDPAALGKALADDPRLLEHLPVSALPERLGRPLPSWADVWTPA
                     APDRTPWADGRVPLLAVGGTRDPFAADTLALWRNWGGPARLVLGPWGHGLTAEPAPEAR
                     PGHRTDLGRLYVRWARAALAPTGRPGAHGAVALGGSEHWYPVTPQDTAPDAGTALPFVS
                     SGSGTGPRLLRGRDFTADPDRPMRSDRLDTDPRAHPDRALLLTPPLPRPLDLLGPACAR
                     LRVAADTPAADWFVRLVALDPRGRAEPLALGTARRTDPPGAPVTFTVPLGFLARRLGAG
                     TRLRVEIGGHHFPAHARNPHTGDDPTTATRLRPSRRTLHTDGSALLLPVRTGRRPFTDP
                     LQEIRT"
     gene            complement(8032..9258)
                     /locus_tag="AB852_RS36565"
                     /old_locus_tag="AB852_35975"
     CDS             complement(8032..9258)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AB852_RS36565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AB852_35975"
                     /product="hypothetical protein"
                     /protein_id="WP_073795749.1"
                     /transl_table=11
                     /translation="MNSVRLAPGVALVTVPGRGLAVRTPGGEFLKVATGEVRETDLRRR
                     LHAPDGTPDGPAAASADGDGPSGGTDPALDALVAAFEGAGYATREPLGTGMLTGCQVLL
                     LGDPVLVDPLMRCARDEGAEVGTITPDRLPRTARRSGGSGRTVVVWCLDGPVPPGLWDE
                     ADRLPGHSVAWLRCHREGDTAWLEPLAATTDDVTSAHVRLRRLAATAAHHELGAYWASL
                     SAEGPGSGHSTASAALTAGLLMEDLVAWASGDRSARRRLRRVRVRRLTVSRHPVLPVPE
                     VAPYPTSAPASALPVPTDPASAEPPRNASAPAMPTAPESGTPRSVPPESGTAVLPARES
                     GTPAPASAASESDGPAPASIAPESDKPVPAVPAPEPASTTRAPASTVTGPPRSRAAVAA
                     PRTAGRAPR"
     gene            complement(9534..9671)
                     /gene="amiA"
                     /locus_tag="AB852_RS40150"
     CDS             complement(9534..9671)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129278.1"
                     /locus_tag="AB852_RS40150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_178391211.1"
                     /transl_table=11
                     /translation="MDQQSVFSPIADQSQLAHLSATHSNALVENPFDDAPVDGDADTEK
                     "
     misc_feature    9738..9751
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.25"
                     /tool="antismash"
     misc_feature    complement(9738..9753)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.71"
                     /tool="antismash"
     misc_feature    9741..9756
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.4"
                     /tool="antismash"
     misc_feature    complement(9743..9756)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.43"
                     /tool="antismash"
     misc_feature    complement(9743..9757)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: strong, score: 24.93"
                     /tool="antismash"
     misc_feature    9744..9757
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.43"
                     /tool="antismash"
     misc_feature    complement(9744..9759)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.48"
                     /tool="antismash"
     misc_feature    9747..9760
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.9"
                     /tool="antismash"
     misc_feature    9747..9762
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.55"
                     /tool="antismash"
     misc_feature    complement(9749..9762)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.31"
                     /tool="antismash"
     misc_feature    9749..9763
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.67"
                     /tool="antismash"
     misc_feature    9751..9764
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 11.5"
                     /tool="antismash"
     misc_feature    complement(9752..9765)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.01"
                     /tool="antismash"
     misc_feature    9752..9766
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.77"
                     /tool="antismash"
     misc_feature    9752..9768
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.21"
                     /tool="antismash"
     misc_feature    9753..9765
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.2"
                     /tool="antismash"
     misc_feature    complement(9753..9766)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.62"
                     /tool="antismash"
     misc_feature    complement(9754..9767)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.3"
                     /tool="antismash"
     gene            complement(9755..10606)
                     /locus_tag="AB852_RS36570"
                     /old_locus_tag="AB852_35980"
     CDS             complement(9755..10606)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847812.1"
                     /locus_tag="AB852_RS36570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_35980"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_342748523.1"
                     /transl_table=11
                     /translation="MSGEHPGRTGTDGEERDMIQLEEVRFGYPGHEVLRGVTLHVTEGE
                     SVALVGLNGCGKSTLLRLCAGLLRPSAGRVLLGGDDLTRLSRRAAARRVALLHQSAPAV
                     PGMTVRQLVRQGRYADRGPLGMLRDSDDPVCARAMADVGVTGWADRPVDSLSGGERQRV
                     RLAMALAQGTRVLLLDEPTTYLDLRHQLEVMGLVARLRDERGLTVVMVLHDLGHAARFA
                     DRIVALRSGTVAADGPPREVVTPALLADVLGVVGRVGRDPEGGWPVCYPDHPVKDETPL
                     VK"
ORIGIN
        1 gacctcggaa gcccaagcgg ctcaaccccg ctgctcccgg tccagcaccc gggtgaacgg
       61 accgggtacg gcggtgagtt ccgcccacgt gccctcctgg gccacgcggc cgtcctccag
      121 caccgcgatc cggtccgccc gccggatcgt cgccgcccgg tgggcgatga tcagcgtggc
      181 gcagtccggc agcgcggcca gcagctcggc gtcaccgagc ccgtcgaggt tgctggtggt
      241 ctcgtcgagc accagcaccc tggggcgcgc gatcagcgcc cgcgccagcg ccacccgcgc
      301 gcgctgaccc ccggagaggg tcgacccgcg ttcaccgagc atcgtggcga acccctgcgg
      361 cagtccgtcg gcgatccggt cgacaccggt gagcgcggcg accgcgacca ggtccgcgtc
      421 ggaggccgcc ggggtgccca gccgcaggtt ctcgcggagg gtgccgtgga acagcggggc
      481 gtcctggccg acgaccgcca cggccgcccg cagctcggat tcggcgacct ggcgcagatc
      541 cacgctccgg ccgtcaccgg ccagcagttc gacccggccg tcggccgggt cccagaagcg
      601 ggccagcaga tgcgcgcagg tcgacttgcc cgcaccggac gccccgacca acgccaccgt
      661 ctcgccggga cgtacgagca ggtcgagacc gtcgaggaca ggcccgccgc cgtacccgaa
      721 gcggacgcca ctgagccgga agcccagcgg accgtcgggg acgggccggg gcgaacgggg
      781 ttcgggcgcg ccccggggcg cccggaccgc cgcgcccacc cgggcggcgg cggcacgcag
      841 acaggcggcc tggccgagcg cggcggcgga gtcggccacg ggtgccagca ccccgagggc
      901 gagggccacg gccgccggga cccacgcgcc ttccagcgtg tgcgcggaca ccgcgacgac
      961 ggcgaccagc gagatcacca ccaggatgtc ccggacggcc gcggaccgtg cctcccgggt
     1021 ctgctccgcg cgctgggcgg cgccgagcag ccgcccgtac ccgcgcaagt ccgcgcggcg
     1081 ccggtcgaac gcgccgaacg ccagcagttc cggcaggccg tcgacggtct cgaccgcccg
     1141 cgccgacagt tccgccgccg ccgaccgggt gcgcgcgccg agcgcggcgc ggtcgcggcc
     1201 ttccagcagg gggcccagca tcagcgcggc ggccatcggc agcaccgccg gcagcagcca
     1261 gggttcgagg tcggcgagaa ccaccgtgcc cgcaccgaac acggctgtgg tggccaggag
     1321 ttgggcgacg gcgtgggcgt agaagaactc cagcgcctcg acgtcggcca gcgcggcgga
     1381 cgccaggtcc ccgctgcggc ggccgccgat ccgcaggggc gcgctgcggg cgagcccgtc
     1441 gaagacccga acgcgcagtt cggccagcac ccggtaggcg aggtcatggg agaggtccat
     1501 ctcccaccag gtggtcgccg cgcggacgag gacgagcgcg acaagggcga ggacggtgcc
     1561 ggtgccgggc gcggcgcggg tcatgacggc ggtgccgacg gtgtgcgcgg cgagcaccac
     1621 aagggcggcg agcgcagtct gtccggcgag cgcggcgacg aaggtccgta cggtcatgga
     1681 gcgatgggcg ccgagcaccg gcaacagggc gcgcagcgaa ccgcgttccg gcgagggact
     1741 cgcaggagaa ccgggaccgg gggcggcacc gggttcggtg gtgcggtggt ccatggtcat
     1801 gccgcttccc ccgcctcgac cagtcgtgcg tacatcccgt cggcggccag cagggcggtg
     1861 tggtccccga cggcttcgac ccggccgtcg ccgtcgagca ccacgatccg gtcggcgtgc
     1921 cgtacggagg cgagccggtg ggcgacgacc agacaggtcc ggccgcgtgc ggcacgcacc
     1981 agttcgcgga cgatcccggc ctctcggtgt tcgtcgacgg cgctggtggc ctcgtcgagg
     2041 acgaggaccg gcgcgtcggc gagcagggcg cgggcgaggg cgagccgctg acgctgcccg
     2101 ccggacaggg tcgagccgcg ttccccgatc acggtggcgt agccgtcgga cagggcgagg
     2161 atctcgtcgt ggacgcccgc cgcgcgggcg gcggcgcgca gttcgccgtc ggtggcgtcc
     2221 gggcgggcga ggcgcaggtt ctcggcgacg gtgtcgtgga agaggtacgt gtgctgggag
     2281 accacggcga tctggcggcg gagcgaaccg agggtgtggg cgccgacctc ggcgccgtcg
     2341 agcaggacgc gtcccccgcc gggttcatgg tgccgcagca gcaggccgac gagggtcgac
     2401 ttgcccgcgc cggaaggtcc gacgatcgcg gtggtgcgtc cggcgggggc ggtgaagctg
     2461 acaccgcgca gggcgggccg tccggtgccc gggtaggtga accgcacgtc gtcgaagcgg
     2521 atctccggcc cctgcccggg gcccgcggtg aggtgtccgg tgtcggggac gccgccgggg
     2581 gcggtgcgca gcgcggcgat cccgtcggcg gccgagacgc cgaggtagcc ggagtgccat
     2641 tcccgggaga gatcgcgcac gggccggaag cactcggcgg tgagcagcag gacgaggtac
     2701 gtcgaggtgg ccgccggatg tccggcggcg gcggaccagc aggcgacgag gacggcggcg
     2761 acggtgccgc cctggacggc gaggtcggtg atcccggtgt cgacgagcga gacccgcagt
     2821 ttggcgacgg tggcccggtg cagcgtgtcc gagcgttccg cgagccgggc ccgcacctgg
     2881 ccgaccgcgc ccagcgcgcg cagcgcgggc atgccctgga gcgcctccag gtagtcggcg
     2941 gccagggcct cgtaggcgcc ccagtgccgc tccccgcggc gtgccagcag ccggtcccac
     3001 cagcgggggg cgaacagggc gagcagcagc gcgggcgcca gtacggcacc cgcccacggt
     3061 tcgaggacgg tgacggcgac gagtggcggc aggggcagca cacaggtgac caggaactgg
     3121 ggcagatagc gggagacgta cgcgtcgacc ccttcgacgc cgtcgacgag ggtggcgcgg
     3181 acggcacccg agcggacgtc ggtgtgccgg gccggtccgg tgcggccgag ccggaccagc
     3241 agttcgtcac gcagtccgac ccgtacggcc gatccggcgc cgacggcgac ccgccgctgc
     3301 caccgggtga gcagcgcgcg ggccgcgacg gccgccagga cggcggcgag cagcgggggc
     3361 aggccggtga ggtccccgct cgccaccgcg ccgaggacga gggcgagcag gacggcctgg
     3421 aggaggtggg tgcaggcggt cgcggcgagc agggcggtcg cgaggagcag cgggcggcgg
     3481 gcggtccggg ccgcgcgcag cagctccggg tggagcatca tcgggtgttc gtctcctgag
     3541 tcgtacgggc ggtgcgggcg gtgccgtcct cggaggccgc cggggtgccg acggccagtg
     3601 cgtgcacgat ccagtcgcgg cccggcggtc cgccgagacg ggcaccgagg tcggcctgct
     3661 gccaggagcc gacgggccgg gacaccaggc cgagggcggt ggcggtgagc tgggcccgac
     3721 cggcgccgta cccggcgagg aggtgttcgc ggcggacgcg ggcgggggtg gcgtcccagg
     3781 ggcagccgtg ggcgaggagg acggcgccgg tggcggcgat ccaccgctgt ccggcgcccc
     3841 agatggcgag ggtgggcagt acgggtccgg tggcgagggt ggtcagcgtg tcgccctcaa
     3901 gtcgtagaag gccgggtgcg gggccgccca cggccacgca ccagcggggc ccgtcggggg
     3961 cggccgacgc ggccgcttcc aggagcaggg cgagcgcggc ggacgagggc cggccggcgg
     4021 tcgcgaggac gtccggatcg gcactgcggc ggccgaggag gaccgcgtcg ggcgcggcgg
     4081 gggcgcgggg cgtggtccag ctcccggtgc cttccccggc cgccgcgagc cgcgcgagga
     4141 cccgcgccgc ctcggcgagg tcgtcgtcct ggggggatcg tgtccccggg ctcgcggagt
     4201 cacgagccgg ggcgggtatc ggggcgggta cgggtgcctg ggcgggcgcg ggtacgggtg
     4261 cctgggccgg tacgggtacc ggagccggtg ggggtacggg tgcgggtccg gatgcgcctg
     4321 ccgtttccgg tacgcaccag gggtccaggg tgccgtccct ggtcagccgg accacggcca
     4381 ggacggcgcc gccaggttcc gtggcggggc ggtccgtcga agggtggtcc gtcgaggggc
     4441 gggcagccgt ggcacggccc ttcgcggtgc ggcccgtcga gaggccgaca gccgtggcac
     4501 ggcccgtcgc cgtgcggggc agcagcacgc ggccgtccag gtcgagggtg aacgccggtg
     4561 cgccggccgc cacgagggcg gccaccgtgt gcccggtgtc cagcaggacc agcggccagc
     4621 cgcggtgccg gtagtgggcg acggtgcgtt ccgcccgcgc gtggagcacc gcgaacaccc
     4681 cgggcggggc ggggacgggc gcggcggcga cccgctggag ccggtgttcg gcgggccggt
     4741 acagatagcg gccgggacgc accccgctgc cagcgcccat cagcaggagc acatcgacgg
     4801 ggtgcagggc tccggcggac gcggcgggcc gcagccgggc cccggcgacg gatcgcgcga
     4861 gcagccggtc gagtgcggcg ggtacggcca ccagggctcc aggggctcgg cggtgggcgg
     4921 ggggccggag gtccggtccg gggacggccg aggagcgggc ccgcgcgagc gcgccggata
     4981 tcagggtggg catgccgggc tcacatgtgc ggaggcggga cgagggtgaa gtcgcccggt
     5041 ccgcgcggtg gtcgggagcg ccagccgcgt gcctcggccg cgtgggcgag gcgggggcag
     5101 ccgaggtacc cgaaggcggc cggggcgttg gggacgagtc cggagacgag gacccgggcg
     5161 accctgagtt ccgtctcggc gatgtcctcg gtggtcaggt cgagggtgac cgtccggtgg
     5221 ccgccctcgg cgaggcgcgc gtcgagacgc gtccggctgc cggtggcgat gtcgtgcacc
     5281 gggaccgtgc cgagcgcggg gtgggtgaag cggcgggcca ggggcgccgt ccgggggtcg
     5341 agccacacct gtacatgggc gccgaggtcc cgtacggacg cgaactcggg tccgcagtcg
     5401 tcgaggtagc ggcggtcggc gcggtgctcc aggtacagac cgcgggccag ggtcccggcg
     5461 tcgaccgcgc ggaacaccca gccgtcgagg tcgagggtgc cgagggtgaa cacccaggtg
     5521 tgcacggctt ccaggacggc cttgcgtacc gcttcggcgg ggtcgtagcg gcaggcgaag
     5581 cccgccgcgt acagatcgcg gacggggtcg tacgccagcg cggcgacgca cggcgcgaac
     5641 tcggagggca tctcgacggc gctgatctcc agtccgccgc ccgcgagttc ccgctccagg
     5701 ccggggacgc tcgcgaggtc gatgccccgg gtgggcccgt cgagccgcca ccacacctcc
     5761 agggcgtccc gctccaccac ttcgagcagc ccgcgttcca cggcgtcgtc gagtccctgg
     5821 ccggtggcga tgcccgcgta gttgaggtga tgggtgcgcg ggaggtcccg cagggcgccc
     5881 tggcgccagt tgaggtgggt cagcgcgacc ggcgcccaca cttccgtacc ggcttcgtcg
     5941 ccgcgtgccc aaagggcggg ggtgtcgtcg gtgaggggcc ggtaggggaa gccggggcgg
     6001 gcgtactgcc agggggcgta ggcgggcagg tcccccggtc cgtacaccgt gcggccctcg
     6061 gcgcgcagtt cggcggccgt ggcccggcgc ggggcggccg gatggccggg gggtggcagc
     6121 cggttgccgc agtagcgttc caccgcttcg gcgacggcgg cggtccgagc gccgtccggg
     6181 tcaccgaagg tggtgcccag cgagacccgg tcggcgggcc agagcccgaa ccggcgcgcg
     6241 tccgcgacct cggcggtcat cgcggtgtag cggggcggcg cgccggtcgg atggggcacg
     6301 ggggcgaccg agcggacgat tccgcagacc gggtccacga gttggtccag ggacagcggg
     6361 gcggtggtca cgtgcgtatc tcctggaggg ggtcggtgaa ggggcggcgg ccggtgcgga
     6421 cgggcagcag cagggcgctg ccgtcggtgt gcagggtgcg ccgggagggg cgcagccggg
     6481 tggcggtcgt cgggtcgtcg ccggtgtgcg ggttgcgggc gtgggcgggg aagtggtgtc
     6541 cgccgatctc gacccgcagc cgggtgcccg cgccgaggcg gcgggcgagg aagccgagcg
     6601 ggacggtgaa cgtgacgggc gcaccgggag gatcggtgcg gcgcgcggtg ccgagggcga
     6661 ggggctcggc gcggccacgc gggtcgagcg cgacgagccg cacgaaccag tcggcggccg
     6721 gggtgtcggc cgcgacccgc agccgggcgc acgcggggcc gaggaggtcc agggggcggg
     6781 gcagcggcgg ggtgagcagg agcgcccggt cggggtgggc gcgggggtcc gtgtcgagcc
     6841 ggtcggagcg catggggcgg tcggggtcgg cggtgaagtc ccggccgcgc agcagccgtg
     6901 gcccggtacc gctgccgctg ctgacgaagg gcagggcggt tcccgcgtcc ggggcggtgt
     6961 cctgcggggt gaccgggtac cagtgctcgc tgccaccgag ggccaccgcg ccgtgcgcgc
     7021 cgggccgtcc ggtcggggcg agggcggccc gggcccaccg gacgtagagc cttcccaggt
     7081 cggtccggtg gcccggccgg gcctcgggcg cgggttcggc ggtgagcccg tggccccagg
     7141 ggccgaggac gagtcgtgcg ggaccgcccc agttccgcca gagggcgagc gtgtcggcgg
     7201 cgaacgggtc acgggtgccg ccgacggcga ggagcgggac ccgtccgtcg gcccaggggg
     7261 tccggtccgg ggcggccggg gtccagacgt ccgcccatga cggcaggggg cgtcccagcc
     7321 gctcgggcag cgcgctgacg gggaggtgtt cgaggaggcg ggggtcgtcg gcgagcgcct
     7381 tgccgagggc ggcggggtcg gacccggcac ggtcgccgtg ggccgcccac catccggcgc
     7441 gccccagcag cctctccacc ccggacggtt cacgggcgac ggcggcctgg cccacggcgg
     7501 gaaccgcggc tatgaccccg tcaggggccg cggcggaccg gggcacggaa gcggcggggg
     7561 ccggggcgcc ggtggtcgcg ggcggggccg gttccgcgcc gggcacggcg gtgaccagcg
     7621 cgcagtacgc ggcgtaggag gcaccgacgg cgatcacccg tccgtcggac cagggctggg
     7681 cacgaatcca gcgcagggtg tcggcgccgt cgccggcctc gtgccggtac gggtgccagt
     7741 cgccgccgga cgcgtggcgc ccccggacgt cctggacgac ggcggcgaaa ccgtgggccg
     7801 cccaggcgcg ggcctcaccg cgatgaccgc ggcggtcgta aggggtccgg agcacgacgg
     7861 cggggaaggg gccggcccct gcgggcaacc ggacatcggt cgccagcgcg gccccgggga
     7921 cgggaacctg tacggacatg gggccgggtg cagggccggg tgccggaccg ggtgccggac
     7981 cggtcgtacc gtcggtcggc acggtggtcg acgggccgga cggcggggcg gtcatcgggg
     8041 ggcgcggccg gcggtccggg gcgcggcgac ggccgcccgg gaacgcggtg ggccggtcac
     8101 ggtggacgcg ggtgcgcggg tggtggacgc gggttcgggt gcgggcacgg cgggcaccgg
     8161 tttgtcggac tcaggcgcga tcgacgccgg tgcgggccca tcggactccg acgcggccga
     8221 cgcgggtgcg ggcgtaccgg actcccgggc aggcaacacg gccgtaccgg actccggcgg
     8281 gaccgacctt ggcgtaccgg actccggcgc ggtcggcatg gcgggcgcgg aagcgttgcg
     8341 cggcggttcg gcggatgccg ggtcggtggg cacgggtagg gcggaggcgg gggccgaggt
     8401 cgggtacggg gccacctcgg ggaccgggag gaccgggtgt cgcgagacgg tgagcctccg
     8461 tacccgtacc cgccgcagtc gccgccgtgc gctccggtcg ccggacgccc aggcgacaag
     8521 gtcctccatc agcagcccgg cggtgagcgc cgccgaggcg gtggagtgcc cggagccggg
     8581 gccttcggcg gacaaggacg cccagtacgc gccgagttca tggtgggcgg cggtcgcggc
     8641 gagcctgcgc agccgtacat gggcggaggt gacgtcgtcc gtggtcgcgg cgaggggctc
     8701 cagccaggcc gtgtcgccct cgcggtggca ccgcagccag gcgacggagt gcccgggcag
     8761 ccggtcggcc tcgtcccaga gtcccggcgg caccggtccg tcgaggcacc agacgaccac
     8821 ggtacgacct gagccgccgg accgccgtgc cgtgcgcggg agccggtcag gtgtgatcgt
     8881 cccgacctcg gcgccctcgt cccgcgcaca gcgcatgagg gggtcgacga gcacggggtc
     8941 gcccagcagc aacacctggc accccgtcaa catgcctgtg ccgagaggct cacgggtcgc
     9001 gtaccccgcg ccctcgaagg cggcgaccag ggcgtcgagc gccgggtccg taccgccgga
     9061 cgggccgtcg ccgtcggcgg aggccgcggc gggtccgtca ggagtcccgt ccggggcgtg
     9121 cagccgtcgg cgcaggtccg tctcgcgcac ctctccggtc gccaccttca ggaactctcc
     9181 gccaggggtg cggacggcga gccctcggcc ggggacggtg accaaggcga cgccgggggc
     9241 gagtcgcacg ctgttcacgg gggcactcct cgtttcctgt gctggaactg ctcggactgc
     9301 tcggactgct gtgaacgccc ggccacttgg accgcttggg ccactcggac gggcgggctg
     9361 ctcgtgttga aggcaagggg ggccgggccg ggcccgcccg gaaccgtggt ccggacgggc
     9421 ccgtccgcgc gtgtcgccac cgacgtcgcg gggcaccggt cgcgcgaggc gccggtcgcg
     9481 cgaggcgcct gccgcgcggg cggcggcgca actgcccggt ggtggtgccg gtactacttc
     9541 tcggtgtcgg cgtcgccgtc caccggggcg tcgtcgaagg ggttctcgac gagcgcgttg
     9601 gagtgggtgg cggacaggtg ggcgagctgg ctctggtcgg cgatcgggct gaacacgctc
     9661 tgctggtcca tgagtcggga ctccaatcgt ggaaggaaag cccccctccg gtgtcgtccg
     9721 gggggaacat cgatgactct agtgaatatg attttcattt tacaagagga gtctcgtcct
     9781 tcaccgggtg atccgggtaa cacacgggcc agcccccctc cggatcacgc cccacccgcc
     9841 ccaccacacc gaggacatcg gcgagcagcg cgggcgtgac gacctcccgg ggcgggccgt
     9901 cggcggccac ggttcccgag cgcagggcga cgatccggtc cgcgaatcgc gccgcgtgcc
     9961 ccaggtcgtg gagcaccatc accacggtca ggccccgttc gtcccgcagc cgggccacca
    10021 gacccatcac ctccagctga tgacgcaggt ccagataggt ggtgggttcg tcgagcagca
    10081 gcacccgggt gccctgcgcc agcgccatcg cgagccgtac gcgctggcgt tcacctccgg
    10141 agagcgagtc gaccgggcgg tccgcccagc cggtcacgcc cacgtcggcc atggcgcggg
    10201 cgcacacggg atcgtcgctg tcgcggagca tcccgagcgg gccccggtcc gcgtacctcc
    10261 cctgccgtac gagctgacgt accgtcatgc cgggtacggc gggtgccgac tggtggagca
    10321 gcgcgacacg gcgtgcggcg gcgcggcggg agagccgggt gaggtcgtcg ccgccgagga
    10381 gcacccggcc ggccgacggg cgcagcagtc cggcgcagag ccgcagcagg gtgctcttgc
    10441 cgcagccgtt gagtccgacc agggcaacgg actcgccctc ggtgacgtgc agcgtgaccc
    10501 cgcgcaggac ctcgtggccg ggatagccga accgcacctc ttccaactgg atcatgtccc
    10561 gctcctcgcc gtccgtcccg gttcgcccgg ggtgctcacc gctcatgtcc cctcctccct
    10621 cgaccgccgc gccaccagga gcagcagcac cgcgccgacg ccggtcgtca gcgcgccggt
    10681 gggcacgctg agccggtcgg tgtccgtcat gtcggccagc agccgggaca gccactgggc
    10741 cgcggcgtcc gccccgcaca ccacgcccgc gccgagcagc gcggcgccgg gcagcgcgac
    10801 gcgcaatccg gcgccgaaca gcgcgaaggc cagatggggc accaggagcc ccacgaagcc
    10861 gagcgccccg acggccgcca ccgcgcccgc ggtcagcgcg accgcgcaga cgagggccgc
    10921 cacgcgggcc gggccggtgc gcagtcccag ggccgcggcc tgttcgtcgc cgcagcgcag
    10981 cagggtcagc ggggcggcga gggtccaggc ggccagggcc cagaggaggg cccagggcca
    11041 cagcagggtc cagtggtccc atacgcggcc ctccgtggag ccgaccagcc actggaccac
    11101 gctgcccagt tcgcccggtg cgacgagcag caccatcgcg gtgagcccgg acagcacggc
    11161 cgacacgagc acgccgtaca cggcggtgcc tacggggtcg tcggcctccc gtccggcgag
    11221 cagccacagc aggcccgccc cggcgaagcc gccgatcatg gcggcggcga ccacggcggc
    11281 cggtgactcc cagcccgcga ggccgagtcc ggtcgcggcg accgcgccga gcaccgcgcc
    11341 cggggtgacc ccggtgacct cgggtccggc g
//
