LOCUS       NZ_LFBV01000007        10972 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces uncialis strain DCA2648 scaffold7, whole genome shotgun
            sequence.
ACCESSION   NZ_LFBV01000007
VERSION     NZ_LFBV01000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces uncialis
  ORGANISM  Streptomyces uncialis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 445914)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Cloning and characterization of the uncialamcin biosynthetic gene
            cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 445914)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33410, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-MAY-2015
            Assembly Method                   :: CLC Genomics Workbench v. 8.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905345.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,456
            CDSs (total)                      :: 7,371
            Genes (coding)                    :: 7,214
            CDSs (with protein)               :: 7,214
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 42 of 157
            Pseudo Genes (incomplete)         :: 143 of 157
            Pseudo Genes (internal stop)      :: 7 of 157
            Pseudo Genes (multiple problems)  :: 33 of 157
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 267420
            Orig. end                         :: 278392
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LFBV01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5972
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(339..352)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.64"
                     /tool="antismash"
     misc_feature    complement(517..532)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            818..2047
                     /locus_tag="AB852_RS25745"
     CDS             818..2047
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /locus_tag="AB852_RS25745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_073792578.1"
                     /transl_table=11
                     /translation="MVEPESRQDTRRVALFDTLLDGRAADDPAAAAAASAALGVPLQDR
                     YTVVVVAQDPAAPPNPGPVLEARGMWSFRRPRAGHYAGIVRLPREGSGPLLDLLRHDMG
                     ATAGLSPEFDRLDRAGRALRLAEQTLRTLPAGRGEAAAFDDRLTGILLVGRPDTAERIV
                     AVHLGPVPATGAEREVPLGTLRVWLDNGCSAARAAEALYCHRSTVLNRIARICELTGVP
                     PESAAARPGWPLAPGPWPLAPGPWPLAPGPARIAPRQGRRNLRGGLRERLTSAAPAGFP
                     GPNSLLVQTSCAAICGAVTFLPGHRGSSGADAPDVTSDGTSYRSYHKGVAFMLKRLGVL
                     AGATVATAGLLMASAPAASADPYFRPHSVYPTSAACHTAGQAGYPLWGLIYKCESFQGR
                     NDAWLLSVRI"
     gene            complement(2167..3063)
                     /locus_tag="AB852_RS25750"
                     /old_locus_tag="AB852_25355"
     CDS             complement(2167..3063)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573984.1"
                     /locus_tag="AB852_RS25750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25355"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_073792579.1"
                     /transl_table=11
                     /translation="MTDALTAVERTSLLTAATRGREHARPDRLYSDPYAARLAGDAGLA
                     LLTEAARIIRPDGRVRAMVSTPDFGVIRTRLFDDFLRAGTTSGRASQIVLAASGMDTRV
                     YRLPWPPGTRLFEIDRPPVLAHKAERLREVAPRADHRTVPADLVTDEWEPALLAAGYDP
                     ARPSLWVLEGLLHYLPPAEVHRLLDRVAAMTAPGSGIAADVAGARALTCESMRPLWELF
                     ARWGSPFRFGDDHPVELFARHGFSVGATEPGAGDTAAYGRWPDPVPDPSRTDVARGFYV
                     RGTRNGLRDDSRDGLPG"
     gene            complement(3060..4643)
                     /locus_tag="AB852_RS25755"
                     /old_locus_tag="AB852_25360"
     CDS             complement(3060..4643)
                     /NRPS_PKS="Domain: AMP-binding (15-413). E-value: 6.2e-83.
                     Score: 270.6. Matches aSDomain:
                     nrpspksdomains_AB852_RS25755_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607979.1"
                     /locus_tag="AB852_RS25755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25360"
                     /product="AMP-binding protein"
                     /protein_id="WP_073792580.1"
                     /sec_met_domain="AMP-binding (E-value: 9.7e-87, bitscore:
                     285.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDQHRFVENTLAVLAARPDAEAVVQGPRRITAGEFRALVLRTAR
                     ALRRRGLRRGDGVALLGRNSPEVIAVRYAAHLLGCRVGHLSTGLTAKALAAGVEDIGAR
                     VLIADPASAGLVAAAPDLAAVPDVLALGPGGHGDDLLALAARESADPVESPTAPDDPCA
                     IHHTGGTTGPPKGICRTYGQIGGIIAAPLPPTLAEVPDGERLLVCTTLTMFGGFLADRV
                     LARGGTVVLQDGFDAAAVLDAVERERVTRLFLPPPLLYALIDHPAVGDHDLSGLRVLLY
                     GASPASPARLADALRTLGPVLVQHYGQNEAGSISLLAAADHDPARPELLRSVGRPLPGV
                     SVEIRDPAGRVLPTGRVGEIWVRSPGLMSGYWKRPDLTAEALRDGWLRTGDLGRSGPTG
                     LLTLTDRVKDVIVVDTVNVYTTEIEHTLDAHPEVLHSAVYGVPDADGVEEVHATVVRAP
                     GAAVDAEALRAWARREQSSVYRLARVAFTDRLPLTEGGKPDKRLLRRLTGTPAGSLPGT
                     PATSLPGDRT"
     aSDomain        complement(3405..4598)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AB852_RS25755_AMP-binding.1"
                     /evalue="6.20E-83"
                     /label="AB852_RS25755_AMP-binding.1"
                     /locus_tag="AB852_RS25755"
                     /protein_end="413"
                     /protein_start="15"
                     /score="270.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="AARPDAEAVVQGPRRITAGEFRALVLRTARALRRRGLRRGDGVAL
                     LGRNSPEVIAVRYAAHLLGCRVGHLSTGLTAKALAAGVEDIGARVLIADPASAGLVAAA
                     PDLAAVPDVLALGPGGHGDDLLALAARESADPVESPTAPDDPCAIHHTGGTTGPPKGIC
                     RTYGQIGGIIAAPLPPTLAEVPDGERLLVCTTLTMFGGFLADRVLARGGTVVLQDGFDA
                     AAVLDAVERERVTRLFLPPPLLYALIDHPAVGDHDLSGLRVLLYGASPASPARLADALR
                     TLGPVLVQHYGQNEAGSISLLAAADHDPARPELLRSVGRPLPGVSVEIRDPAGRVLPTG
                     RVGEIWVRSPGLMSGYWKRPDLTAEALRDGWLRTGDLGRSGPTGLLTLTDRVKDVIVV"
     CDS_motif       complement(3501..3587)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AB852_RS25755_0001"
                     /evalue="1.60E-11"
                     /label="NRPS-A_a6"
                     /locus_tag="AB852_RS25755"
                     /protein_end="381"
                     /protein_start="352"
                     /score="36.4"
                     /tool="antismash"
                     /translation="PTGRVGEIWVRSPGLMSGYWKRPDLTAEA"
     misc_feature    complement(4976..4989)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.47"
                     /tool="antismash"
     gene            5001..5972
                     /locus_tag="AB852_RS25760"
                     /old_locus_tag="AB852_25365"
     CDS             5001..5972
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="AB852_RS25760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25365"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_073792581.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-11, bitscore: 39.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSCDQTSLLGAPAPGHPCRYDTVPPRLVDRERPENVLFASAAF
                     DGERITAVPRPTAGHPYFGDRPEGQDGVDLRTILEMGRAAAFLAFEAVGDRRAGMCVAI
                     ARARLIRPAPSVVPAPRPGEPGGPGGYVMRCARLRDTFWSFAATHSTGSPGAPSAGSCE
                     GRGTILDSRRLAALRAEERRLRIAALRAERPDDGPAGSATPGEVGRRRPENVFVAGVRH
                     TPDGTRALLRVPATHPVVHDPAYDHLPALALADAALQLVHARHGGRVEVREIDLRPTRF
                     CETDLPVRLSLGSTGTRPGELRFEAVQGGMTVARAQLVTVGV"
     gene            6127..7224
                     /locus_tag="AB852_RS25765"
                     /old_locus_tag="AB852_25370"
     CDS             6127..7224
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005476127.1"
                     /locus_tag="AB852_RS25765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25370"
                     /product="saccharopine dehydrogenase"
                     /protein_id="WP_073792582.1"
                     /transl_table=11
                     /translation="MNHAPHDQLTHDPDGPVLLVGGYGTVGAELTRMAAPHWPLLLAGR
                     TVARGADLARETGATTVRWDLADPEPFASAVRAVVSVVNDPDDRVLRAALDGGVPYVDL
                     TRWTARVQRAVTVAALRRPTAPVLLSSAWMGGVISLAVAALAHELGGADTVDVAIRWDL
                     NDRAGTDSVEFMDRLGLDYEIVEDGRRRSVAPLGDARRVRIGAFPTRVARIDTPEQFTL
                     PLTLGTTTAVTRIGFDSAASTTALLAAKRIGFFRWGRGDRFTSARRSLLYAPGDGGTAL
                     VRVDVTHGGRTRTATLTDPAGQAHLTAVGGLLGLRRVLGADGAPAPTGVVLPEQTPLPD
                     GVAAALAAQGVTLDTARDRTAERAA"
     gene            7221..7844
                     /locus_tag="AB852_RS25770"
                     /old_locus_tag="AB852_25375"
     CDS             7221..7844
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151816.1"
                     /locus_tag="AB852_RS25770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25375"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_073792583.1"
                     /transl_table=11
                     /translation="MTGPPPARPPAGPAGTGKGERKRGELLDAAETVLLASGYPELSMR
                     AVATAAGVRLGHLQYYFPTRPDLVAALLDRVLRRSLERLDPLLAAAPETLDATGLVRAL
                     LADQDDPALVRLFTELWALAALDETVATAVRAFYHDYRERLTEHIRRHRPALPDADRRA
                     RAEVFIMLIEGASLFRSGITSRRSPESDARLLATAVALLTAPAD"
     misc_feature    complement(8029..8043)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     misc_feature    complement(8034..8048)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.62"
                     /tool="antismash"
     gene            8039..8638
                     /locus_tag="AB852_RS25775"
                     /old_locus_tag="AB852_25380"
     CDS             8039..8638
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /locus_tag="AB852_RS25775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25380"
                     /product="L,D-transpeptidase"
                     /protein_id="WP_073792584.1"
                     /transl_table=11
                     /translation="MTIKSSTKRNTVAAVAALGVAFSVTAAGQAGAQPAAAPAEQQSAA
                     APSYYLKFDKGTVTNSRLSLMRSVSGPDRVIKSYKAGSGTSTNTCAVGKGRLPNGKYRI
                     EFRRTDFDGLINGYVIKVTDKRCHNGTKRTELFIHSEMKPNGGQGRIESEKWTDTNPND
                     YYSNGCIKLNPKNIKALFAKADSLGWSRVKGLVVTS"
     gene            8813..10819
                     /locus_tag="AB852_RS25780"
                     /old_locus_tag="AB852_25385"
     CDS             8813..10819
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487181.1"
                     /locus_tag="AB852_RS25780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_25385"
                     /product="hypothetical protein"
                     /protein_id="WP_073792585.1"
                     /transl_table=11
                     /translation="MSELPLPLPAEHIPPGTRDWRGTDTARWAAAAPARWTHPLWAVAA
                     LSAAAVWSIAAAPDDICTVAQPCDADWQYVALSLVAVLTLYWTWRQPRLALAGLAVTAG
                     GALLDAGPTGLVTGPQDVAMLGALVFALLTLAHRVAARRRQRSLAEAAAGPERHRLPAE
                     VRGFRRGRVSFVMASLLLAVSLFALWRGQSVVTGYEDRAERGTRTTAQVTEVDGDDDGA
                     TMKVTLPDDSTRRLTVLFPEDHPVGSTVVLVVDGGWTRLVAEPYDIVGWQLLLLGTAVP
                     GLAFLANGLTGQRRQRRLDTTPLPVLKVLVREGEDDGRTWVYAADDLTALRPVLSFHSL
                     YAVDDPDDPIDAYPEDMAGGERPGPAADHMEAVRWEQEIAEAVAACQDTEPPLREAVLY
                     GSPRAGGEIAFLAMADDGDELEAERSVTPVKPVGVGLFPRTTWRASTRPGDPGDPGDPG
                     DPGDLGDPGGAGGPEPTRGPRRTRSIAETARGMTRTTDPLVWSADRGSRIVGLLLLLVQ
                     GGGTWAILEDGFSWQSLLLVIGLPWLVHSVSTALNWRVTADRDGVWIAGAWRVRRLRWD
                     EIAGVHQGSDSIRFSLTGGRDVELSPTGAAVIQQMRGGTAAATRAVDTLRVLRHHPELR
                     PSRDADPAEQGMPLGPLVVTAAVLWGVAVLMLN"
ORIGIN
        1 ccgccgtccg cgccctcacc cacgccatgg accaggcgcg gagcgcgggg tccgaccact
       61 acaccaccga acacctgctg cgcggcctcc tcgccgacgg caccagccgc gccgcccgga
      121 tgctcaccct ctgcggcacc gacgccaccg aggtgctccg acgcctcgac gagggcgact
      181 tcgagcccgc cgacgacgga ctgcccccgc agctgtggcc cacccgcgac accctcctcg
      241 ggcgccgctc accgaccgcc acctccctgt ggcgaaggct gctcctgcgc ctcgccaagg
      301 gcgccaacct cgccgcttcg cccgtcagct ggctcggttt cgacgcggtc cagcaggcgg
      361 gcaccctgaa gcagccactc ggcaccgagc acctgctgct gtccgtgctc gccacccacg
      421 aggtcgccgt cagatacccg cacctcgcca tggcgggcgc gtccgccgac ggtgacggcg
      481 ccgacgtggc cgaccggttc gcgggcggcg cggcgctgca cgccctcggc gtcgactacc
      541 tcaccgtgcg ccgcgccgtg gagcgggacg gccatcagct gggcgaggac gaccgggacc
      601 ccgacacgta tctggaggcc ttcgagggcg gggacggtac cggcccgctc acccggtcgc
      661 tcctccacgg cgagaaccgg gcccggcgtc tgctgatcac cctgggcttc cccgtctgaa
      721 ccgcggggcc gggcccagcg gcacggcgtg gtcagcgatg ggacgtaccg cgtccgggcg
      781 ccaacgtacc gcggggctga gcacaggtcg ggcgcgggtg gtggagccgg aaagccgtca
      841 ggacacccgg cgggtcgccc tgttcgacac gctcctcgac ggtcgcgcgg cggacgaccc
      901 ggccgcggcg gccgccgcgt cggccgcgct cggcgtgccg ctccaggacc ggtacacggt
      961 ggtcgtcgtc gcccaggacc cggcggcccc gccgaatccg ggaccggtcc tcgaagcccg
     1021 gggcatgtgg tcgttccggc ggccccgggc cggccactac gcgggcatcg tccggctgcc
     1081 gcgcgaggga agcggcccgc tgctggacct gctgcgccac gatatgggcg cgacggccgg
     1141 actctcgccg gagttcgacc ggctcgaccg ggcgggccgt gcactgcggc tcgcggaaca
     1201 gaccctgcgg accctgcccg ccggacgcgg tgaggccgcc gcgttcgacg accggctcac
     1261 cgggatcctg ctcgtcggcc gtccggacac cgccgaacgg atcgtcgccg tgcatctggg
     1321 cccggtgccg gccaccgggg cggaacgcga ggtgccgctc ggcaccctgc gggtgtggct
     1381 cgacaacggc tgctcggcgg cccgggccgc cgaggcgctc tactgccacc gcagcaccgt
     1441 cctcaaccgc atcgcccgga tctgcgaact cacgggcgtc ccaccggagt ccgccgccgc
     1501 tcgtcccggc tggcccctgg cccctggccc ctggcccctg gcccctggcc cctggcccct
     1561 ggcccctggc cctgcgcgca ttgccccccg tcaaggccgc cgaaacctcc ggggcgggct
     1621 tcgcgaacga ctgacatccg ccgccccggc cggctttccg ggaccgaact ccctgctggt
     1681 ccagacctct tgtgcggcca tctgcggtgc cgttacgttt ctgcctggtc acagggggtc
     1741 gtcaggggcg gacgcgcccg atgtgacttc tgatggcacg tcatatcgct cgtaccacaa
     1801 gggagttgcg ttcatgctga aacgtctcgg tgtcctcgcc ggagccactg tcgccaccgc
     1861 cgggctcctc atggcatccg cgcccgccgc cagtgcggac ccgtacttcc gcccccacag
     1921 cgtctacccg acctcggccg cgtgccacac ggcgggccag gcgggctacc ccctgtgggg
     1981 cctgatctac aagtgtgaat cgttccaggg caggaacgac gcctggctgc tgtcggtccg
     2041 catctgaccg tccgccgtcc ggcccggccg ccgctgtcgg acgcgccggg ccggacggcc
     2101 cccggcctcc gggccggacg gaaccatgag cggacggctc gccgggcacg ggggccggcg
     2161 agccgctcag cccggcagac cgtcgcggga gtcgtcccgc agcccgttcc gtgtccctcg
     2221 gacgtagaag ccgcgcgcca cgtcggtgcg cgaggggtcg gggaccgggt cgggccagcg
     2281 gccgtacgcc gcggtgtcac cggcgcccgg ctcggtggcg ccgacgctga agccgtgccg
     2341 ggcgaacagt tccacggggt ggtcgtcccc gaagcggaac gggctgcccc agcgggcgaa
     2401 cagctcccac aagggacgca tcgactcaca ggtgagggcg cgggccccgg cgacatccgc
     2461 ggcgataccg ctgccgggcg ccgtcatcgc ggccacccgg tcgagcagtc ggtgcacctc
     2521 ggccgggggc agatagtgca gcagcccttc gaggacccac agcgagggcc gcgccggatc
     2581 gtagcccgcg gccagcagcg cgggctccca ctcgtcggtg accaaatcgg cggggaccgt
     2641 ccggtgatcg gcgcgcggcg cgacctcgcg cagccgctcc gccttgtggg cgagcaccgg
     2701 gggccggtcg atctcgaaga gccgcgtacc cggcggccag ggcagccggt acacccgggt
     2761 gtccatcccg gaggcggcga gcacgatctg cgaggcgcgg ccggacgtgg tgcccgcacg
     2821 caggaagtcg tcgaacagcc gggtgcggat gaccccgaag tcgggggtgc tgaccatcgc
     2881 ccggacccgt ccgtccggcc ggatgatccg ggccgcctcg gtgagcagcg cgaggcccgc
     2941 gtcgcccgcg agccgggccg cgtaggggtc gctgtagagc cggtcgggcc gtgcgtgctc
     3001 acggccccgg gtggccgcgg tgagcagcga ggtccgctcg accgcggtca gtgcgtcggt
     3061 catgtccggt ctccaggaag agaggtggcc ggggtgcccg gaagggaacc ggccggggtg
     3121 cccgtcagcc gtcgcagcag tcgcttgtcg ggtttgccgc cctcggtgag cggcagccgg
     3181 tcggtgaagg cgaccctcgc gagccggtac acggaactct gttcccgccg tgcccaggcg
     3241 cgcagcgcct ccgcgtcgac ggccgcaccg ggcgccctga cgaccgtcgc gtggacctcc
     3301 tcgaccccgt cggcgtccgg caccccgtac accgcgctgt gcagtacctc ggggtgggcg
     3361 tcgagcgtgt gctcgatctc ggtggtgtac acgttcacgg tgtccaccac gatgacgtcc
     3421 ttgacccggt cggtgagcgt cagcaggccc gtggggccgg accgtccgag gtccccggtg
     3481 cgcagccagc cgtcgcgcag ggcctcggcc gtgaggtcgg gccgcttcca gtagccgctc
     3541 atcaggccgg gggagcgcac ccagatctcc ccgacccggc cggtcggcag gacgcggccc
     3601 gccggatcgc ggatctccac cgacacaccg ggcagtggtc tgccgaccga gcgcagcagc
     3661 tcgggccggg ccgggtcgtg gtcggcggcg gccagcagac tgatgctgcc cgcctcgttc
     3721 tgcccgtagt gctggacgag gaccggaccg agcgtgcgca gcgcgtcggc gagacgggcg
     3781 ggggaggcgg gggaggctcc gtacagcagg acgcgcagac cggagaggtc gtggtcgccg
     3841 accgcgggat ggtcgatcag cgcgtagagc agcggcgggg gcaggaacag ccgggtgacg
     3901 cgttccctct cgacggcgtc cagtacggcg gcggcgtcga agccgtcctg gaggacgacc
     3961 gtgccgcccc gtgccaggac ccggtccgcg aggaacccgc cgaacatcgt cagcgtggtg
     4021 cacaccaaca gccgctcccc gtccggcact tccgccagcg tgggcggcag cggcgccgcg
     4081 atgatcccgc cgatctgccc gtatgtacgg cagatgccct tgggcgggcc cgtggtgccg
     4141 cccgtgtgat ggatcgcgca ggggtcgtcc ggcgcggtgg gggactccac cgggtcggcg
     4201 gactcccggg ccgcgagcgc gagcaggtcg tcaccgtgcc ccccgggccc gagtgccagg
     4261 acatcgggga cggcggccag gtccggtgcg gccgcgacga ggccggccga cgcggggtcg
     4321 gcgatcagga cccgggcgcc gatgtcctcg acccccgcgg ccagcgcctt cgccgtcagt
     4381 ccggtggaca gatgcccgac ccggcagccg agcagatggg ccgcgtaccg gacggcgatc
     4441 acctcgggcg agttccggcc gagcagcgcc accccgtccc cccggcgcag cccgcggcgc
     4501 cgcagcgccc gcgccgtccg caggacgagg gcacggaact cgcccgcggt gatccggcgc
     4561 ggcccctgca ccacggcctc ggcgtccgga cgtgcggcga gtacggcgag agtgttctcc
     4621 acgaagcggt gctggtcggg catgggtccc ttccgggggc gggccgggga ccggccgaca
     4681 gggcggggca cccggggcgc ggtgtcgcgc gggcatgggg gagagcgggt ccgggcggcg
     4741 gaggaacccg cctcaaggcg ggcacacccc accgggcgga cacaacgtag tcagccggcg
     4801 acattcagtc aaaaggcggg gtcgcgccgc gcacggggtg cggggtcagc cgaacggatg
     4861 acgcgcgggc cccggccggg tcccccgcgc ccggcctcgc cgggaatccc cgcgcgccgt
     4921 gcggcggcgc cgtgttgacg cgacggaagt gttccccgaa caggcgctac gtgaaggaag
     4981 gtttgctcgt ttgcccacac atgaccacgt cttgtgacca gacctccctg ctcggcgccc
     5041 ccgcgccggg tcacccctgc cggtacgaca cggtgccccc gcgtctcgtc gaccgggagc
     5101 ggccggagaa cgtcctgttc gcctccgcgg ccttcgacgg agagcggatc accgccgtac
     5161 cgcgtcccac cgccggtcac ccctacttcg gtgaccggcc ggagggtcag gacggagtgg
     5221 atctgcgcac gatcctggaa atggggcgcg cggcggcgtt cctggccttc gaagcggtgg
     5281 gcgaccggcg cgccgggatg tgtgtcgcca tcgcgcgggc ccggctgatc cgtccggcgc
     5341 cgtccgtcgt cccggcgccc cgccccgggg agcccggtgg cccgggggga tatgtgatga
     5401 ggtgcgcacg gctccgggac accttctggt ccttcgcggc gacgcactcc acggggtccc
     5461 ccggcgcccc ttcggccggg agctgcgaag ggcggggcac catcctcgac agccgtcggc
     5521 tcgccgcgct gcgtgccgag gagcggcgac tgcggatcgc ggcgctgcgt gctgaacggc
     5581 cggacgacgg cccggccggg agcgcgaccc cgggggaggt cggacggcgg cggccggaga
     5641 acgtgttcgt ggccggggtg cggcacacgc ccgacgggac gcgtgcgctg ctgcgggtcc
     5701 ccgcgaccca cccggtggtc cacgaccccg cttacgacca cctccccgcg ctcgcgctgg
     5761 cggatgcggc gctccaactc gtccacgcgc gccacggggg gcgcgtcgag gtgcgggaga
     5821 tcgatctccg gccgacccgg ttctgcgaga ccgacctgcc ggtacggctg tcgctcgggt
     5881 ccaccgggac gcgcccgggc gagctgcgtt tcgaggccgt ccagggcggg atgaccgtgg
     5941 cccgtgccca gttggtgact gtgggcgtgt gaagcggtga cccgcgccgg ggcgggcagg
     6001 ccgggaggca cccgcccccg gcgcctcccg tcgacgcacc cccgctgcgg ctcccgagcc
     6061 gtctccctca catcgattag gtcatgcgac ctaaagttat aggtcgcatg acctatagtg
     6121 gattccatga accacgcacc acacgaccag ctcacccatg accccgacgg ccccgtcctg
     6181 ctcgtcggcg gatacggcac cgtcggggcg gaactcaccc ggatggccgc accgcactgg
     6241 cccctcctgc tcgccggacg gaccgtggcg cgcggcgccg acctggcccg cgagaccggc
     6301 gcgaccaccg tccgctggga cctcgcggac cccgaaccct tcgcgtcggc cgtccgcgcg
     6361 gtcgtcagcg tcgtcaacga ccccgacgac cgggtactgc gcgcggcgct cgacggcggg
     6421 gtgccctatg tggacctcac ccgctggacc gcccgcgtgc agcgcgcggt caccgtcgcg
     6481 gccctgcgcc ggccgaccgc cccggtcctg ctctcgtccg cctggatggg cggcgtgatc
     6541 agcctcgcgg tggccgccct cgcccatgaa ctcggcggcg ccgacaccgt ggacgtggcg
     6601 atccgctggg acctgaacga ccgggcgggc accgactcgg tcgagttcat ggaccggctc
     6661 ggcctggact acgagatcgt cgaggacggc cggcggcgca gcgtcgcacc gctcggggac
     6721 gcccgccgcg tccggatcgg cgccttcccc acccgggtcg cccggatcga cacccccgag
     6781 cagttcaccc tcccgctgac gctcggcacc accaccgccg tcaccaggat cggcttcgac
     6841 tccgccgcct ccaccaccgc gctgctcgcc gcgaaacgga tcggcttctt ccgctggggc
     6901 cgcggtgacc gcttcacctc cgcccgccgc tccctgctct acgcgcccgg cgacggcggc
     6961 accgcgctcg tacgtgtcga cgtcacccat ggcggccgca cccgcaccgc gaccctcacc
     7021 gacccggccg gccaggcgca cctcaccgcc gtcggcggcc tcctcgggct gcgccgggtc
     7081 ctcggagcgg acggagcccc cgcccccacc ggtgtcgtcc tgcccgaaca gaccccgctc
     7141 ccggacggcg tcgccgccgc tctcgccgcc cagggtgtca ccctcgacac cgcgcgggac
     7201 cggaccgcgg agcgcgcggc atgacgggcc cgccgcccgc ccggcccccg gcgggccccg
     7261 ccggaaccgg caagggcgaa cgcaagcgtg gtgagctcct ggacgccgcc gagacggtcc
     7321 tgctcgcctc cggatacccg gaactctcca tgcgggccgt cgccacggcc gccggggtac
     7381 ggctcggcca cctccagtac tacttcccga cccgccccga cctcgtcgcc gcgctcctcg
     7441 accgggtgct gcgccgctcg ctggagcggc tggaccccct gctcgcggcg gcccccgaga
     7501 cccttgacgc caccggactg gtccgcgccc tgctggccga ccaggacgac ccggccctcg
     7561 tccggctgtt caccgaactg tgggcgctcg ccgcgctcga cgagacggtc gccaccgccg
     7621 tccgggcctt ctaccacgac taccgggagc ggctcaccga gcacatccgg cggcaccgcc
     7681 ccgccctgcc cgacgcggac cgccgggccc gcgccgaggt gttcatcatg ctgatcgagg
     7741 gggcgtccct gttccgctcg ggcatcacct cccggcgctc gccggagagc gacgcccggc
     7801 tcctcgccac cgccgtcgcc ctgctcaccg caccggccga ctgacaccgc acgcccgccg
     7861 tacgcactcc cgcccgccgt acgcactccc gcccggcggc ccgcccggac ccgtccaagg
     7921 tttccccaag gtttcctccc agtccgcccc ggcatggtcg acgtgtcgcc ccggagcagc
     7981 accaccgggg ggcatccggc tccgcaacgg accaccgaat ccagggggat cgaggaacat
     8041 gacgatcaag tcgagcacca agaggaacac cgtcgccgcc gtcgcggcac tcggagtggc
     8101 gttctccgtc accgccgccg gtcaggcggg cgcccagccc gccgccgcgc cggccgaaca
     8161 gcagtccgcc gcggcaccga gctactacct caagttcgac aagggcacgg tcaccaactc
     8221 ccggctgtcg ctgatgcgga gcgtcagcgg tccggaccgg gtcatcaagt cctacaaggc
     8281 gggctccggc acctcgacca acacgtgcgc ggtcggcaag ggccggctgc ccaacggcaa
     8341 gtaccgcatc gagttccgcc ggaccgactt cgacggcctc atcaacggct atgtcatcaa
     8401 ggtcaccgac aagcgctgcc acaacggcac caagcgcacg gagctgttca tccacagcga
     8461 gatgaagccc aacggcggcc agggccggat cgagtccgag aagtggaccg acaccaaccc
     8521 gaacgactac tactccaacg gctgcatcaa gctcaacccg aagaacatca aggcactgtt
     8581 cgccaaggcg gactccctcg gctggtcccg ggtcaagggc ctggtcgtca ccagctagcc
     8641 acccgggtgg cgtgacaccg cggcggtttc cgacagcggc ccgccggagc cctcacccgg
     8701 ccccggcggg ccgcgccacg ccgcacaccg cccctgagcc ctcgcgacca tgatcgcgct
     8761 cgcaggccgg acccgcggac agcccgcgag ccggtcgcac gataatacgg acgtgagcga
     8821 acttcccctt ccgctgccgg ccgagcacat accacccggc acccgtgact ggcgcggcac
     8881 cgacaccgcc cgctgggcgg ccgccgcccc cgcccggtgg acccatccgc tgtgggcggt
     8941 ggccgcactc tccgccgccg ccgtgtggtc gatcgccgcc gccccggacg acatctgcac
     9001 cgtggcccag ccgtgcgacg ccgactggca gtacgtggcc ctgtcgctcg tcgccgtact
     9061 gaccctgtac tggacatggc ggcaaccgcg tctcgccctg gccggtctcg ccgtgaccgc
     9121 gggcggcgcg ctgctcgacg ccggacccac cggtctggtc acgggccccc aggacgtcgc
     9181 catgctcggc gccctcgtct tcgcgctgct caccctcgcc caccgcgtcg ccgcccggcg
     9241 ccgccagcgc tccctcgccg aggccgccgc cggacccgag cgccaccggc tgcccgccga
     9301 ggtacgcggc ttccggcgcg gccgggtctc gttcgtcatg gcctccctgc tgctggcggt
     9361 ctccctcttc gccctctggc ggggacagtc ggtcgtcacc gggtacgagg accgcgccga
     9421 gcggggcacc cgcaccaccg cccaggtcac cgaggtggac ggcgacgacg acggcgccac
     9481 gatgaaggtg accctgcccg acgacagcac ccgccgtctc accgtcctct tccccgagga
     9541 ccaccccgtc ggcagcaccg tcgtcctcgt cgtggacggc ggctggacac gcctggtcgc
     9601 cgagccgtac gacatcgtcg gctggcaact gctgttgctc ggcaccgccg tgcccggcct
     9661 cgccttcctc gccaacggac tcaccggaca acgccgtcag cgccgcctcg acacgacccc
     9721 gctcccggtc ctgaaggtcc tcgtacgcga gggcgaggac gacggacgca cctgggtcta
     9781 cgccgccgac gacctcaccg ccctgcgccc cgtcctcagc ttccactcgc tgtacgccgt
     9841 cgacgacccc gatgacccca tcgacgcgta ccccgaggac atggcgggcg gcgaacggcc
     9901 cggcccggcg gccgaccaca tggaggccgt gcgctgggaa caggagatcg ccgaagccgt
     9961 cgccgcctgc caggacaccg aacccccgct gcgcgaggcc gtcctgtacg gctccccccg
    10021 cgccggcggc gagatcgcct tcctcgccat ggcggacgac ggagacgaac tggaggcgga
    10081 gcgcagcgtc acccccgtga agcccgtggg cgtgggactc ttcccccgga ccacctggcg
    10141 ggccagcacc cgcccgggag atccgggaga tccgggagat ccgggagatc cgggcgacct
    10201 gggcgacccg ggtggcgccg gcggccccga gcccacccgg ggcccccggc gcacccgctc
    10261 gatcgcggag acggcccggg gcatgacccg caccaccgac cccctcgtct ggtccgccga
    10321 ccggggctcc cggatcgtcg gactgctgct gctcctcgtg cagggcggcg gcacctgggc
    10381 catcctcgaa gacggcttct cctggcagtc gttgctgctg gtgatcgggc tgccctggct
    10441 cgtccactcg gtgtccaccg ccctcaactg gcgggtcacc gccgaccgcg acggggtctg
    10501 gatcgccggg gcatggcgcg tccgccgctt gcgctgggac gagatcgccg gtgtccacca
    10561 aggctcggac agcatcaggt tcagcctgac cggcggacgg gacgttgaac tgtccccgac
    10621 cggcgccgcg gtcatccagc agatgcgcgg cggcacggcc gcggcgaccc gcgccgtgga
    10681 caccctgcgg gtgctgcgcc accaccccga actccggccc tcccgcgacg ccgacccggc
    10741 ggaacagggc atgccgctgg gcccgctggt cgtcacggcg gcggtgctct ggggcgtcgc
    10801 cgtcctcatg ctgaactgac cgctgaccgc tgaccgctga ccgctgaccg tggctggtgg
    10861 ctggtgggac gtgggacgtg ggaccttggc ccgggtccgg tggcttcagg ccgtcggtac
    10921 cgggcccggg aacccggcgg gcgcctccag caccgtcacc acctcgtggt gc
//
