LOCUS       NZ_LFBV01000002        11491 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces uncialis strain DCA2648 scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_LFBV01000002
VERSION     NZ_LFBV01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces uncialis
  ORGANISM  Streptomyces uncialis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1356271)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Cloning and characterization of the uncialamcin biosynthetic gene
            cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1356271)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33410, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-MAY-2015
            Assembly Method                   :: CLC Genomics Workbench v. 8.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905345.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,456
            CDSs (total)                      :: 7,371
            Genes (coding)                    :: 7,214
            CDSs (with protein)               :: 7,214
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 42 of 157
            Pseudo Genes (incomplete)         :: 143 of 157
            Pseudo Genes (internal stop)      :: 7 of 157
            Pseudo Genes (multiple problems)  :: 33 of 157
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 975229
            Orig. end                         :: 986720
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LFBV01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11491
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11491
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11491
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6491](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6491
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    128..142
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            complement(170..1726)
                     /locus_tag="AB852_RS12450"
                     /old_locus_tag="AB852_12190"
     CDS             complement(170..1726)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192952.1"
                     /locus_tag="AB852_RS12450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12190"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_073787010.1"
                     /sec_met_domain="DAO (E-value: 1.5e-07, bitscore: 27.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYDYVVVGAGSAGCVLAARLSEDPGTRVALIEAGGADTAQDIH
                     VPAAFSRLFKSDLDWDLDSEPEPGIGGRRAYLPRGKVLGGCSSINAMIYIRGNRADYDG
                     WAAAGAEGWSYDDVLPYFRRSEDNTRGADAFHGTGGPLTVSDSRSNHPLVTSFVEAAVK
                     AGHPRNDDFNGGSQLGVGPYQLTQRDGLRCSTSVAFLHPALERPNLTVVTGARADRVVI
                     ENGRATGVEVTRDSGTEVITAGREVILSAGAYGSPKLLMLSGIGPAAALTALGIEVVAD
                     LPVGEGLQDHYMTMLNFRTDTESLMTAASPDNAALLQGEGRGPLTSNVGEAGGFFHSRD
                     GLTAPDLQFHMAPVLFHQEGLGAVTEHGFAFGPCVLAPTSRGRVTLRSDRPDAAPRITH
                     NYLTTDEDRAALVAGIRIALDIAAQAPAAEVITGPYDVPDSDSDADLLAWAQRSGMTLY
                     HPTSTCAIGPVVDPRLRVHGVEGLRVVDASVFPSVPRANTNAPTVMAAEQAADLIKGAA
                     R"
     gene            1922..3130
                     /locus_tag="AB852_RS12455"
                     /old_locus_tag="AB852_12195"
     CDS             1922..3130
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /locus_tag="AB852_RS12455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12195"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_073787012.1"
                     /transl_table=11
                     /translation="MTTALGADAHITELVGRLLASVDPLTDELTAEILSGEHQYVESTL
                     LSHEQLRDAVHDNLRTLLTALQGRPTSLDAPRAAGRLKAERGIPLAALLHAFRLAGRFI
                     WDRLLVMAVAEDSTTELLHMASGIWVVIDECSGAAAEAYRATVEEQSRRDAAARSVMLT
                     TLLDGSAGNGAGAWETVRVLGLDRQGPFLVVCAEVNDGTEPLPAVDARLRSAGIGSEWI
                     QQIGSRVGLLALPNEHAATTAAEQLARSAVSRVGVSRPFASPAQAPSAWREAQMAAQCL
                     PPGTDGAHVYGSSPIALLAAASPDIAAEVTRTVFGRLRDLPEPEQTLLLDTLETWFTAG
                     GSTTRAAERLHCHRNTVLYRLNQIAALTGRRTTDADSSAELYIALRAVRLAGGTGPRAA
                     GRA"
     misc_feature    complement(3182..3196)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    complement(3596..3612)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 18.89"
                     /tool="antismash"
     gene            3970..5004
                     /locus_tag="AB852_RS12460"
                     /old_locus_tag="AB852_12200"
     CDS             3970..5004
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="AB852_RS12460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12200"
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_143201220.1"
                     /transl_table=11
                     /translation="MRIEPDAGSARSDEDRLDELVAGCLTAAGCAAGTWTPTDLRYPGL
                     HATAHRTQRARRTATPALEQSRTGAPAAAGAEGGAASGALEQTEERVRAMVLRARRSQA
                     RARSVEKRTGWAVDLAPSGEDHLAQSVRRALRQAPAPADGSRGGRTAEVTDWSAEQHEV
                     FEEGCRILQAAWPQMLAELRVTLRQVALLGGWGIDGFTDFTVHGAVFVNSRRLGDQGTE
                     GLAGRFRLVEALVHEGTHNRCDAAAASAAFLRASGAQELASTPLRADPRPLAGLFQQVA
                     VLARSVLLYRRLPDGPAVRDRHAVLLEQGGRGAATLRGRAGALTDAGRELLDQAEAVFA
                     TGAP"
     gene            5001..6491
                     /locus_tag="AB852_RS12465"
                     /old_locus_tag="AB852_12205"
     CDS             5001..6491
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="AB852_RS12465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12205"
                     /product="YcaO-like family protein"
                     /protein_id="WP_073787016.1"
                     /sec_met_domain="YcaO (E-value: 5.2e-28, bitscore: 94.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAVEPLPRSAGSGGSARTAEPGPSPVWPVQVVSPFPDAPRFVLG
                     RTAARSSAFTPNDAAGGRTVLIGAATGTDRADVARRARGELMERMHNVLSGHRAREGAV
                     VASYASLRRRGTAALDPCSWAELRSLTHLREAAMPWVTGTSLTGGGQVLVPACAVYLAH
                     RPPAGCPAPLRPGSTGLAAHDTAARAREHAVLEILERDLFWHAWYADAPRRSPPGPAPL
                     DEELRRVLVVLGLRERCLVLPGPGGISCVVACVHTSDGTRQSFGARATSGALGPAAVSA
                     VREALMVRWSMGTASARTVWARMREQGPDRQPSGPLEHALHTFHRQDSLTALLRGATGE
                     PWTTTARGPVGEPRSTARASAAEPRATAHDSSEDPWMTEPDSAGDPRVPARGSAGDLRV
                     TARGPSGNPSAAGHDPVRDPGVTAPGPAEAAPRLLAERTGEDVVEVDTSVSAADGGRAV
                     VVRVIAPGARRMPVDEPDRAALALPAGNARRHPPHPLG"
     gene            6545..7327
                     /locus_tag="AB852_RS12470"
                     /old_locus_tag="AB852_12210"
     CDS             6545..7327
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /locus_tag="AB852_RS12470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12210"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_073787018.1"
                     /transl_table=11
                     /translation="MSAPVSLQAPGPAEPAGAASYARRFADRYDTWFGRKGLTNDTVGL
                     LARLAGSGTALELGVGTGRIALPLARRGVRVEGVDASPEMAARLAARPGGEHIPVTIGD
                     FTRLPSDGRTVDVVFLAGGTFFELPTQEDQSRCFRHAAALIGDDGAFVLDAIVPEVLIG
                     AEWSRGHVVPETGDDLVVLYRRFDSAAQRYHSHYAITPADGSGNVHIHVAFRYAGAGEL
                     DLMARAAGLELRERYGSWGGAPFRDGCPYHVSVYRPAR"
     gene            7344..7757
                     /locus_tag="AB852_RS12475"
                     /old_locus_tag="AB852_12215"
     CDS             7344..7757
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AB852_RS12475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AB852_12215"
                     /product="hypothetical protein"
                     /protein_id="WP_143201221.1"
                     /transl_table=11
                     /translation="MPDWTVPAWAAPVLTAAGVVLLAAFVFWGARISPNRPFSWAMYSG
                     SSKGFLWSDDGSVTVLPLHRLRIAPGGHFLTVPELRALLADGDFELPLRGLIIGSDGGW
                     EVRYEPAEHRLRLTRLPAGAELRLLADALRPAP"
     gene            7754..8602
                     /locus_tag="AB852_RS12480"
                     /old_locus_tag="AB852_12220"
     CDS             7754..8602
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AB852_RS12480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AB852_12220"
                     /product="hypothetical protein"
                     /protein_id="WP_073787022.1"
                     /transl_table=11
                     /translation="MSIPVLTAALMLALAVVWHAAVVLADARAVTRHRPDGRVYRPGRG
                     WTSAAGLAARRRLSYLMVVAGAVLAGLCLVAGLAPGRGTAMVLGGWAAVVLALLLADRR
                     HGLNRYTAVCLALLAGCLVLAGGAWALGGRTWTAAAGPPASFFAAQLYLVAGVRKLRSR
                     HFMDGGVLIDNLAYNAAQAAAGSHDFVPFPRTAALSTLLVHPAPRSVCRAVAVCAAVGE
                     VLLGLAVLGLLPRPATLTLALVLHSGFLLLSPLRIVPFSAASMGLCVLATSQPLLPGPL
                     G"
     gene            8909..9379
                     /locus_tag="AB852_RS12485"
                     /old_locus_tag="AB852_12225"
     CDS             8909..9379
                     /GO_function="GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015609530.1"
                     /locus_tag="AB852_RS12485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12225"
                     /product="hemerythrin domain-containing protein"
                     /protein_id="WP_073787024.1"
                     /transl_table=11
                     /translation="MSERETTRLVAWSKELRQVHHRLRQALEVTRASLTDGTTGEAATR
                     ELLLYCRGFCAALDSHHKGEDRTLFPAIAAAHPELRPVLRSLEQDHSMIAHLLGGLQAA
                     VERAAPPEELDLHLEGISALMENHFRYEERQLLTVLETLELTAAPGEVLGPL"
     gene            complement(9639..10277)
                     /locus_tag="AB852_RS12490"
                     /old_locus_tag="AB852_12230"
     CDS             complement(9639..10277)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541844.1"
                     /locus_tag="AB852_RS12490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_12230"
                     /product="LysE family translocator"
                     /protein_id="WP_073787026.1"
                     /transl_table=11
                     /translation="MLTAFASFALFALLVTVVPGPDTLLVLRNCVRGGRLPGIATALGA
                     AVGSLVWAVAAAVGLAAALHRWDAAFAVVRMAGAAYLVLLGAQALWAHRRSASNGADTT
                     AAPPEGLAPTTFLAFRQGLLSCLLNPKVGLFFVAVAPQFLPEGASVLSVTLIFGVIDAV
                     IAAGWLILVAVFAARLLSWLRRPRVNTALERATGGVLVVLGLGTAAETL"
ORIGIN
        1 gcggcgcgca cccaggccgc cgcgtgctca ccggcggcgg tgtggacgcg ttccagcagt
       61 tcgcggcggc gggcgagtcc gagggcggcc caggcggact cgccccgtac gaggtcggcg
      121 acggcgcggt cggcctcggc cagccggtcg gcggcgggtg cggaggtggt catcgcgcgg
      181 ctcccttgat gaggtcggcg gcctgctcgg cggccatgac ggtgggcgcg ttggtgttgg
      241 ccctggggac cgaggggaag acggaggcgt cgacgacccg cagcccctcc actccgtgca
      301 cccgcagccg ggggtcgacg acggggccga tggcgcaggt cgatgtcggg tggtagagcg
      361 tcatgcccga acgctgggcc cacgcgagca gatcggcgtc cgagtcggag tccgggacgt
      421 cgtaggggcc ggtgatgacc tcggcggccg gggcctgggc cgcgatgtcc agggcgatcc
      481 ggattccggc gacgagggcg gcgcggtcct cgtcggtggt caggtagttg tgggtgatcc
      541 ggggcgcggc gtccgggcgg tcggagcgca gggtcacccg gccgcggctg gtcggtgcga
      601 ggacgcaggg cccgaaggcg aagccgtgct cggtgacggc gccgagtcct tcctggtgga
      661 agaggacggg ggccatatgg aattgcaggt ccggggcggt gaggccgtcg cggctgtgga
      721 agaagccgcc cgcctcgccg acgttcgagg tgagggggcc gcgtccctcg ccctggagga
      781 gggcggcgtt gtccgggctg gccgccgtca tcagggactc cgtgtcggtg cggaagttca
      841 gcatcgtcat gtagtggtcc tggagtcctt cgcccaccgg cagatcggcc accacctcga
      901 taccgagggc ggtgagcgcg gcggcgggtc cgatcccgga cagcatcagc agtttcgggg
      961 acccgtacgc cccggccgac aggatcacct cgcgtccggc ggtgatcacc tcggtcccgc
     1021 tgtcccgggt cacctccacc ccggtggccc ggccgttctc gatcaccacc cggtccgcgc
     1081 gggccccggt caccaccgtg aggttcggcc gttccagggc cgggtggagg aaggcgacgg
     1141 aggtgctgca ccgcagtccg tcgcgctggg tgagctgata ggggccgacg ccgagctggc
     1201 tgccgccgtt gaagtcgtcg ttgcgagggt gtcccgcctt gacggccgcc tcgacgaacg
     1261 aggtgaccag ggggtggttc gaccggctgt cggagacggt cagcggaccg cccgtgccgt
     1321 ggaaggcgtc cgcgccgcgc gtgttgtcct cggagcggcg gaagtagggc aggacgtcgt
     1381 cgtacgacca gccctcggca ccggccgccg cccagccgtc gtagtccgcg cgattgcccc
     1441 tgatgtagat catcgcgttg atcgagctgc atccgccgag caccttgccg cgcggcagat
     1501 aggcgcggcg cccgccgatg cccggttcgg gctcggagtc gaggtcccag tcgaggtcgc
     1561 tcttgaacag cctgctgaag gccgcgggca catggatgtc ctgggccgtg tccgcaccgc
     1621 ccgcctcgat cagcgccacc cgcgtcccgg ggtcctcgga cagccgggcg gccaggacac
     1681 agcccgctga tcctgccccg acgacgacgt agtcgtaggt gttcatccga tctccttcgg
     1741 ccggctctga tccgtcccct gacctccggg cgactgtgcg ggcgccacat ggagggatcg
     1801 aggggaacgt cgatgaccgg aatgtttgcc gtgataccgg tgcaccgcca taggcggtgg
     1861 gcgggagtaa ccggccggtc tttgtgcccg cgcacaatcg ccgagggtct accctcgtcc
     1921 catgacgaca gcgctgggcg ccgacgcgca catcacggag ctggtcggcc ggctgctcgc
     1981 gtcggtggac ccgctgacgg acgagctgac cgcggagatc ctcagcggtg agcatcagta
     2041 cgtggagagc accctgctca gccatgagca gttgcgggac gccgtgcacg acaatctgcg
     2101 gacgctgctc accgcgctcc aggggcggcc gacctcgctg gacgcgcccc gcgcggcggg
     2161 ccggctcaaa gccgagcggg gcattccgct ggccgccctg ctgcacgcct tccggctggc
     2221 cgggcggttc atctgggacc ggctgctggt gatggccgtg gcggaggaca gcaccaccga
     2281 gctgctgcac atggcgtccg gtatctgggt ggtgatcgac gagtgctccg gcgcggcggc
     2341 cgaggcgtac cgggcgaccg tggaggagca gtcgcgccgg gacgccgccg cccgcagcgt
     2401 catgctcacc accctcctcg acggcagcgc cgggaacggc gcgggggcct gggagaccgt
     2461 acgggtgctg gggctggacc ggcagggccc cttcctggtg gtgtgcgcgg aggtcaacga
     2521 cggcaccgaa ccgctgcccg ccgtggacgc gcggctgcgg tccgccggga tcggctcgga
     2581 gtggatccag cagatcggca gccgggtcgg gctgctcgcg ctgccgaacg agcacgcggc
     2641 gacgacggcc gccgagcagc tggcccggtc ggccgtgtcc cgggtcgggg tgagccgccc
     2701 cttcgcgtcc ccggcgcagg cgcccagcgc ctggcgggag gcccagatgg cggcccagtg
     2761 cctgccgccc ggcaccgatg gcgcccatgt ctacggcagc tcaccgatcg ccctgctggc
     2821 ggcggcctca ccggacatcg cggcggaggt gacccgtacg gtgttcgggc ggctgcgcga
     2881 tctgccggag ccggagcaga ccctgctgct ggacaccctg gagacctggt tcacggccgg
     2941 cggttcgacc acacgcgccg ccgagcggct gcactgccac cgcaacaccg tgctctaccg
     3001 gctgaaccag atcgccgccc tgaccggccg ccgcaccacg gacgcggact cctccgccga
     3061 gctgtacatc gcgctgcggg cggtacggct ggcgggcggc accgggcccc gggcggcggg
     3121 gagggcctga cgggggcgtg acgaccggat gctcgcggga ggcgggtgcg cggtccggtg
     3181 gggggccggt cggcgcgggt cggcgcgggt gcgggaagcg ggtccggtcg gcgcgggtgc
     3241 gggtccggtg cgggcggttc ccgggggtcg gggacggccg gttcccgtcg ggccctcccc
     3301 ggttcccggg ggtccttgac cgggcaacca actgccgcgc gcggcggccc gcctcaccgg
     3361 tcggccctgc cgttcgacgt gagccctctc cgctaccggc cgcggaaccg tcggcacagc
     3421 gggggcggcc ccccgccggg tgggccggag ccggacgggt accggccatg ggcggatgac
     3481 cgaagacttg ggtttgggcg ggggctgcgc gggccaccgg atgcgcgggc cgcgcggcgg
     3541 cgggtcggcg gggcactcct gtggccgatg agacgggtgg caggccgggg aaatgtgccc
     3601 ggttcacatg cgaacgacgg cgactgccgc gagcggtcga gaacctgccg gggccggcgc
     3661 cggtcgcatt gtccggcggg cgcgttgctc agctcccgtc cggactgtca ccgtcggtgg
     3721 gcgccttcgc cacacgcggt gagggccgta cctcctccga ggacaaggag tactccgtgg
     3781 aaatggaaga ggcacgtgag ctggacatcg aggcggtgga cggggaggaa ctccccgagt
     3841 tcgcgctgat ggtcgacgtc ccggactgat caccgggtga acggcggccg ggccgtgctc
     3901 gtcggcccgg cctgccggtc ggggcggatc acgcggatcg accactggga cggcttgtca
     3961 ggagggtgtg tgcggatcga gccggacgcc gggtcggcca ggagcgacga ggaccggctg
     4021 gacgagctgg tggcaggctg tctcaccgcc gccggttgcg ccgccggcac ctggacacct
     4081 accgatctgc gctatcccgg gctgcacgcc acggcacacc ggacgcagcg ggcgcggcgg
     4141 acggccacac cggccctgga gcagtcacgc accggggcgc ctgccgccgc gggagcggaa
     4201 ggcggggccg cctccggcgc tctggagcag accgaggagc gggtgcgggc gatggtgctc
     4261 cgcgcccggc gcagccaggc ccgtgcccgg tccgtggaga agcgcaccgg atgggccgtg
     4321 gatctggcgc cgtccggcga ggaccatctg gcacagagcg tccggcgcgc gctgcggcag
     4381 gctcccgccc cggccgacgg gtcccggggc gggcgtacgg ctgaggtgac cgactggagc
     4441 gcggaacagc acgaggtctt cgaggagggg tgccgtatcc tccaggccgc ctggccgcag
     4501 atgctcgcgg aacttcgggt gacgttgcgt caggtggcgt tgctgggcgg ctgggggatc
     4561 gacgggttca ccgatttcac cgtgcacggc gctgtcttcg tcaacagccg tcgactgggc
     4621 gaccagggca ccgaagggct cgcgggccgg ttccggctcg tggaggcgct ggtgcacgag
     4681 ggcacccaca accgttgcga cgcggccgcc gcctccgccg cgttcctccg ggcctccggc
     4741 gcgcaggagt tggcgagcac accgctgcgc gccgatccac gcccactggc cggactgttc
     4801 cagcaggtcg cggtgctggc acgcagtgtg ctgctgtacc ggcggctgcc ggacggaccc
     4861 gccgtccgtg accggcacgc cgtcctgctg gagcagggcg gccggggcgc ggccacgctg
     4921 cgcggccggg ccggcgccct gaccgacgcc ggacgcgagc tgttggacca ggccgaagcg
     4981 gtcttcgcga cgggagcgcc atgagcgcgg tcgaacccct cccccggtcc gcggggtccg
     5041 gcgggtccgc ccgtaccgcc gagccggggc cgtccccggt ctggccggtg caggtggtgt
     5101 cgccgttccc cgacgcgccg cggttcgtgc tcggccggac cgccgcgcgc agcagcgcct
     5161 tcacgccgaa cgacgcggca ggcgggcgta cggtcctgat cggggcggcc accggcaccg
     5221 atcgcgcgga tgtggcgcgg cgggcccggg gcgagttgat ggagcgtatg cacaacgtcc
     5281 tgtccgggca ccgggcgcgt gagggggccg tcgtcgcgtc gtacgcgtcg ctgcggcgcc
     5341 ggggcacggc cgcgctggac ccctgttcct gggcggagtt gcgctcgctc actcatctgc
     5401 gggaggcggc gatgccgtgg gtcacgggca cctcgctgac cggcggcggt caggtgctcg
     5461 tccccgcgtg tgcggtgtac ctggcgcatc gcccgcccgc cggctgtccg gcgccgctgc
     5521 ggcccggttc caccgggctc gccgcgcatg acaccgccgc gcgggcccgg gagcacgccg
     5581 tgctggagat actcgaacgg gacctgttct ggcacgcctg gtacgccgac gcgccccggc
     5641 gttcgccgcc aggccccgcg ccgctcgacg aggagcttcg gcgggtgctc gtcgtgctgg
     5701 ggctgcggga gcggtgcctg gtactgcccg gtcccggagg tatctcgtgc gtggtggcct
     5761 gtgtgcacac gagtgacggc acccggcaga gtttcggggc gcgtgccacc tccggggcgc
     5821 tcggcccggc cgcggtgtcc gccgtacgtg aggcgctgat ggtccgctgg agcatgggaa
     5881 ccgcgagcgc ccgtaccgtg tgggcccgga tgcgggagca agggccggac cggcagccga
     5941 gcggaccgct ggagcacgcc ctgcacacct tccaccgcca ggacagtctc accgcactgc
     6001 tgcgcggcgc caccggggag ccctggacga cgacggcgcg tggtcccgtc ggggaacccc
     6061 ggtcgacagc acgcgcttcc gccgccgaac cccgggctac ggcgcacgat tcctccgagg
     6121 acccctggat gacggagccg gactccgccg gggacccccg ggtgccggca cgtggttccg
     6181 ccggggacct ccgggtgacg gcacgtggtc cctccgggaa cccttcggct gcggggcacg
     6241 atcctgtccg ggaccccggg gtgacagcac ccggccccgc cgaggccgcc ccgcgtctgc
     6301 tcgccgagcg gaccggcgag gacgtcgtcg aggtcgacac ctccgtctcc gcggccgacg
     6361 gcggccgggc ggtggtggtg cgggtgatcg cccccggcgc ccgccggatg cccgtcgacg
     6421 aaccggaccg ggcggccctc gccctccccg ccgggaacgc ccggcggcat cctccgcacc
     6481 ccctcggctg accgcggtcc gcacgctcgt tcccgtccga cctccctgga gactggagat
     6541 ccccatgtcc gcgcccgtgt cccttcaggc ccccggcccg gcggaacccg ccggggccgc
     6601 ctcgtacgcg cggcgattcg ccgaccgcta cgacacctgg ttcggcagga agggcctgac
     6661 gaacgacacc gtcggcctcc tggcgcggct cgccgggtcc ggtaccgcgc tggaactggg
     6721 cgtgggcacc ggccggatcg cgctgccgct ggcgcggcgc ggtgtccgcg tggagggcgt
     6781 cgacgcctca ccggagatgg cggcccggct ggcggcgcgg cccggcggcg agcacatccc
     6841 ggtgaccatc ggtgacttca cccggctgcc gtcggacgga cgcacggtcg atgtggtctt
     6901 cctcgccggg gggacgttct tcgaactccc cacccaggag gaccagtcgc ggtgcttccg
     6961 ccatgcggcg gcgctgatcg gcgacgacgg cgcgttcgtg ctggacgcga tcgtccccga
     7021 ggtgctgatc ggcgcggaat ggtcgcgcgg ccatgtggtg cccgagacgg gcgacgacct
     7081 ggtcgtgctc taccgccggt tcgacagtgc cgcgcagcgc taccactcgc actacgcgat
     7141 cacacccgcc gacggaagcg gcaatgtgca catccatgtc gccttccgct acgcgggcgc
     7201 cggtgaactg gacctgatgg cgagggcggc cgggctggaa ctgcgcgagc ggtacgggag
     7261 ttggggcggg gcacccttcc gggacggctg cccgtaccac gtgtcggtct accggcccgc
     7321 gcgctgaccg ggcgcccccg accgtgcctg actggaccgt gcccgcctgg gccgcccccg
     7381 tgctgaccgc cgccggcgtg gtactgctcg ccgctttcgt cttctgggga gcacggatct
     7441 ccccgaaccg cccgttctcc tgggccatgt actccggctc gtccaagggc ttcctgtggt
     7501 cggacgacgg cagcgtcacc gtcctgcctt tgcaccggct gcggatcgcg ccgggcggcc
     7561 acttcctcac cgtcccggaa ctgcgcgccc tcctcgccga cggtgacttc gaactgccgt
     7621 tgagaggact gatcatcggc tccgacggcg gctgggaggt gcggtacgaa cctgcggagc
     7681 accggctgcg gctgacccgt ctgcccgccg gagccgaact gcgcctgctg gccgacgcct
     7741 tgcgcccggc cccgtgagca tccccgtact caccgccgcg ctgatgctcg ccctcgccgt
     7801 ggtctggcac gccgccgtcg tcctggccga cgcccgcgcc gtcacccggc accgtcccga
     7861 cggccgggtc taccgtcccg gccgcggttg gacctccgcc gccgggctcg ccgcccgccg
     7921 ccgtctctcg tacctcatgg tcgtggcggg cgcggtcctg gccggtctct gcctcgtcgc
     7981 ggggctcgcc ccggggcgcg gcacggccat ggtcctgggc gggtgggccg ccgtcgtcct
     8041 ggcgctgctg ctcgccgacc gccgccatgg cctcaaccgg tacaccgccg tgtgcctggc
     8101 gctgctcgcg ggctgtctcg tgctggcggg cggcgcctgg gccctcggcg gccggacatg
     8161 gacggcggcg gccggtccgc ccgcttcgtt cttcgccgcg cagttgtacc tggtcgccgg
     8221 ggtacgcaag ctgcgctcac ggcatttcat ggacggcggc gtcctcatcg acaacctcgc
     8281 ctacaacgct gcccaggccg ccgcgggcag ccatgacttc gtccccttcc cgcgtacggc
     8341 cgcgctgagc accctgctgg tccaccccgc gccgcgctcc gtctgccgtg ccgtcgccgt
     8401 ctgcgcggcc gtcggcgaag tcctcctggg actcgccgtc ctcggcctgc tcccccggcc
     8461 cgccaccctc accctggccc tcgtcctgca ctcgggattc ctgctgctga gccccctgcg
     8521 catcgtgccg ttctcggcgg cgtcgatggg cctgtgcgtc ctcgccacgt cccagccgct
     8581 gctcccgggg cccttgggct gacggccacg ggtcgggcgg aagtgccagg gacagctgtc
     8641 gccgccggag cggcgggaag ggtgcgatgc ggctcccacg gcgcaccggc ccacgaggcc
     8701 gttcgtcgga cgcgggacag gacgcggcgt cccttgtcag cggggcccgg agcacacccg
     8761 gtgggcggaa cggcggcggc cgtgccgccg gggcagcggt cggggtattc tgcgggaagc
     8821 gtcgcgtgcc ggtggcagac cgtgtcaggc atggaggcgt ccgaccgaag gaggctggcg
     8881 ttgaacacgt cgcacccttc gttctcgcgt gtctgagcgc gagacgacca ggctcgtggc
     8941 ctggagcaaa gaactccgtc aggtgcacca ccggctgcgg caagcgctgg aagtgacccg
     9001 cgcgtccctc actgacggca ccaccggtga ggccgccacc cgtgaactgc tgttgtactg
     9061 ccgcggtttc tgcgcggctc tcgacagcca tcacaagggc gaggaccgca ccctgttccc
     9121 ggccatcgcg gccgcccatc cggagctgcg cccggtactg cgttcgctgg agcaggacca
     9181 ttcgatgatc gcccacctgc tgggcggcct ccaggcggcg gtcgaacggg ccgcgcctcc
     9241 cgaggaactc gatctgcacc tcgaaggcat ctccgcgctc atggagaacc acttccgtta
     9301 cgaggaacgg cagttgctca ccgtgctcga aacccttgag ctgacagccg cgcccgggga
     9361 ggtgctgggc ccgctgtgat cccggaccgg accggtgggg ggcggtggcc gtggcggtca
     9421 ccgcccccac cggtcggtcg gcgccgtcgc gctccgccaa aggccgatca ctcgctcccc
     9481 ggccgcttcc ccggacggga gaggacgacg gcccttggta cgttcccgct ccccatggcc
     9541 cggtcgtccg ggccgtccga cccgacgccc cgtcagcaca ggttcccccg tccggcgacg
     9601 gctgggcatg tgcggcacct gcggcccacg cggcacgctc acagcgtttc cgcggcggtc
     9661 cccagtccga ggacgaccag gacgccgccg gtcgccctct ccagcgccgt gttcacccgg
     9721 ggccggcgca gccagctcag cagacgggcc gcgaagaccg ccaccaggat cagccagccg
     9781 gccgcgatca cggcgtcgat gacgccgaag atcagggtca cggagagcac ggaggcgccc
     9841 tcggggagga actgcggtgc cacggcgacg aagaagaggc cgaccttggg gttgaggagg
     9901 cagctgagga gcccctgccg gaacgccagg aaggtggtgg gtgcgagtcc ctcgggcgga
     9961 gcggcggtgg tgtccgcgcc gttcgacgcg ctccggcggt gcgcccacag ggcctgcgcg
    10021 cccagcagga cgagataggc cgcgcccgcc atccgtacga cggcgaacgc ggcgtcccag
    10081 cggtggagcg cggcggccag ccccacggcg gcggcgaccg cccagacgag ggaaccgacc
    10141 gcagcgccca gcgcggtggc gatccccggg agtctgccgc cacgcacgca gttgcgcagc
    10201 accagcaggg tgtccggtcc gggcaccacc gtcaccagca gggcgaagag agcgaagctc
    10261 gcgaaggctg tgagcacgga ggcttcctgt tccgacgtcg gctgccgagc gtgctgtcga
    10321 acgcccggcg tcgaggctat cggcctggtg aggccccgcg ccgcggagat ccgcgaagcc
    10381 cccgggaacc tcctcgactc ccgccccggc ggtcacccgc catccgtgac gcgggtacgg
    10441 gatcggcaca tgccccgttg cttccccttc gcccggggcc gggcccggag cgcgaaccgg
    10501 acttcgggcg cagcggcgga cgcggcggtg ccgaccacta cgggccctgc ggaccaaccg
    10561 ggctacctat cagcgctgtt ggccgccaca ggtgccctcg accgtacgtc cggtacggaa
    10621 gaaggtgatc gccttgtcgg cgcagggcga cgagggcagg gtcgcgtgcc cgtcgtccag
    10681 gagggtgagc agggcgcctc cggtggcgtc cctggcccgt tcggcccaga cgtgaggagt
    10741 gacgttctca tagaggtggc cggagagctg aagcgcgctc ttgccgtacg cgggcttggc
    10801 ggcgggcacc ttgtacggcc acttcgcgca ccaggggctg aagtgcgcac ctcccgtcaa
    10861 gggatcggcc tcacgacggg cctcgcgggc cgcccacagt tcgccgaaac cgcggctcgc
    10921 ggcggcggtg ttgcagagga tcgcgttgta ctggagtgtg ttgaagcggc tgcgcggatt
    10981 gctcagcccg agaggacgta ccggcgccgg tgcggcggtt tccgcccggg gcgccggtgg
    11041 cgtggggggc gtgccgccgt cgcgcaccgc gaccaggctc ttcgccgcgt cggcccactc
    11101 ctcctggggg cggtagacct gctcgatcac ccagttcccg tccaggcgta ccccgccacc
    11161 ggtgcgcggc ttccgctcca gctcgctgcg cagatcaccc agccgactgc ggacggtgga
    11221 ctcgtcggcg cccaggtggt gctcggcgtc gcgctgtgcc agccaggcga ggaacggcgc
    11281 ggcgctccgg ttgccggccg cctccgtctc gccgtccatc gtgggccagt ggaccgtcgg
    11341 gggcaacacc gagtccagcc acatgcgttt cgtgtgacgg tcgaacaggg aacggtaggc
    11401 cattccgatg gcggtaccga aggaggcgcc gtagaagttg atcttctccg cgccgagtgc
    11461 ggcccggagc cggtcgatgt cccgggcggc g
//
