LOCUS       NZ_LFBV01000002        10372 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces uncialis strain DCA2648 scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_LFBV01000002
VERSION     NZ_LFBV01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces uncialis
  ORGANISM  Streptomyces uncialis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1356271)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Cloning and characterization of the uncialamcin biosynthetic gene
            cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1356271)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33410, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-MAY-2015
            Assembly Method                   :: CLC Genomics Workbench v. 8.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905345.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,456
            CDSs (total)                      :: 7,371
            Genes (coding)                    :: 7,214
            CDSs (with protein)               :: 7,214
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 42 of 157
            Pseudo Genes (incomplete)         :: 143 of 157
            Pseudo Genes (internal stop)      :: 7 of 157
            Pseudo Genes (multiple problems)  :: 33 of 157
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 829442
            Orig. end                         :: 839814
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LFBV01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10372
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="6"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10372
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10372
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5372](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5372
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(1790..1805)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.75"
                     /tool="antismash"
     gene            complement(2179..3693)
                     /locus_tag="AB852_RS11885"
                     /old_locus_tag="AB852_11630"
     CDS             complement(2179..3693)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (24-403). E-value: 3.7e-45.
                     Score: 146.1. Matches aSDomain:
                     nrpspksdomains_AB852_RS11885_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="AB852_RS11885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_11630"
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="WP_073786815.1"
                     /sec_met_domain="AMP-binding (E-value: 2.5e-43, bitscore:
                     144.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHGESPRPAGGSSAAPTLTGCLRAGAERAPDAVAISTRGSAAWQ
                     DMTFAELHDAALTAAANATAWERPAGPVVVLVDNTPTCVATMLGLFLAGADTLLVEREN
                     SHLMEARSVIHRLPPAGLVGPSDISPVPGLAHRTFDELVSVPAPSGTVVRREDPATAVL
                     QLTSGSTGEPRITRQTLGNTLTGGALYRDIHRITAADTVLAMVPLAHSFGLVGALATAL
                     VSGAGLRTMTRFRPGVVLEALDAGSTVLLGTPLVYQLMTPVLPRTGPRGKVRVALSSGG
                     PLTSDSAKAAAARLGVPVHQVYGSTETGLIACRHDRDEPWPEDSAGVMAPGVDWRVRTG
                     CSRTAAHGRGHLLVRTSTMFEGYEDGGGPPSMEDGYLACGDLTEVDEDGHVFVVGRKDA
                     FVNVGGRKVSPRRVENVVAEHPGVSEVLVFGAPSRTGEEEVHAALVLRPDNDAADVLTF
                     CRARLMPYEVPHRVHVLTALPRTGMGKIDRHRLRETVTAGKGTATG"
     aSDomain        complement(2485..3621)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AB852_RS11885_AMP-binding.1"
                     /evalue="3.70E-45"
                     /label="AB852_RS11885_AMP-binding.1"
                     /locus_tag="AB852_RS11885"
                     /protein_end="403"
                     /protein_start="24"
                     /score="146.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="AGAERAPDAVAISTRGSAAWQDMTFAELHDAALTAAANATAWERP
                     AGPVVVLVDNTPTCVATMLGLFLAGADTLLVERENSHLMEARSVIHRLPPAGLVGPSDI
                     SPVPGLAHRTFDELVSVPAPSGTVVRREDPATAVLQLTSGSTGEPRITRQTLGNTLTGG
                     ALYRDIHRITAADTVLAMVPLAHSFGLVGALATALVSGAGLRTMTRFRPGVVLEALDAG
                     STVLLGTPLVYQLMTPVLPRTGPRGKVRVALSSGGPLTSDSAKAAAARLGVPVHQVYGS
                     TETGLIACRHDRDEPWPEDSAGVMAPGVDWRVRTGCSRTAAHGRGHLLVRTSTMFEGYE
                     DGGGPPSMEDGYLACGDLTEVDEDGHVFVVGRKDAFVNV"
     CDS_motif       complement(3169..3213)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AB852_RS11885_0001"
                     /evalue="5.00E-03"
                     /label="NRPS-A_a3"
                     /locus_tag="AB852_RS11885"
                     /protein_end="175"
                     /protein_start="160"
                     /score="10.1"
                     /tool="antismash"
                     /translation="AVLQLTSGSTGEPRI"
     gene            complement(3690..4793)
                     /locus_tag="AB852_RS11890"
     CDS             complement(3690..4793)
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PP-binding (283-325). E-value: 6.9e-10.
                     Score: 31.1. Matches aSDomain:
                     nrpspksdomains_AB852_RS11890_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /locus_tag="AB852_RS11890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="WP_073786818.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1e-11, bitscore:
                     41.5, seeds: 297, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.4e-09, bitscore:
                     32.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAIILTGGKGTRLRPYTENRPKGLLQLSRYSILEVIIRRLRGAG
                     FSHITLCVSHLADQIRAEFGDGATLGVRIDYSTDPRPLGTAAPLAQVADFNEPALVMNG
                     DVLTALDFAELYRVHKDSGGVLTVASFRRPMPIGFGVLDIRGDQVAGIWEKPTLHLDIS
                     AGIYVADPAVLEAITPGKVVDMPDLVGSMMESGRRVHAYSFSDRWHDIGTPERYEEAQR
                     CFAENPDLYLRNTRTPDRSTPEDRPEGARPPELRLYPSDTSREANGSVTNDDPATNDVP
                     SQIAQDMGTVLECRPLGPADDFFLLGGDSLRAVELIARLTERFEPDDAVAADRLHAELL
                     LAVFDDASPDALADVVRSHHAASRSGR"
     aSDomain        complement(3819..3944)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AB852_RS11890_PP-binding.1"
                     /evalue="6.90E-10"
                     /label="AB852_RS11890_PP-binding.1"
                     /locus_tag="AB852_RS11890"
                     /protein_end="325"
                     /protein_start="283"
                     /score="31.1"
                     /tool="antismash"
                     /translation="IAQDMGTVLECRPLGPADDFFLLGGDSLRAVELIARLTERFE"
     gene            5001..5372
                     /locus_tag="AB852_RS11895"
                     /old_locus_tag="AB852_11640"
     CDS             5001..5372
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321937.1"
                     /locus_tag="AB852_RS11895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_11640"
                     /product="ectoine synthase"
                     /protein_id="WP_245876756.1"
                     /sec_met_domain="ectoine_synt (E-value: 1e-25, bitscore:
                     86.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSVEEVVGSECDVDWGNGTSRRLLVQADNLGFSLTETYVQPGSE
                     SYLRYDNHQEVCYCVSGSGSVETEDGVFEIKPGTLYAPGVGEPHVLRSQHGMTLMCVFS
                     PALHGSETHLLTPGVHSSY"
     misc_feature    complement(5011..5026)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.84"
                     /tool="antismash"
     gene            5382..6821
                     /locus_tag="AB852_RS11900"
                     /old_locus_tag="AB852_11645"
     CDS             5382..6821
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (19-299). E-value:
                     6.8e-26. Score: 82.9. Matches aSDomain:
                     nrpspksdomains_AB852_RS11900_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="AB852_RS11900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_11645"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143201217.1"
                     /sec_met_domain="Condensation (E-value: 2.7e-20, bitscore:
                     68.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAETIQCEVAFTGGRFAAGPATCAQRHMWNLIQQRMPDSAFYNQI
                     HWAALPPGGTLSDVREVLGELVGRHESLRTVYQVGGDGGLRQRVIRSGSFTVGTLKAAD
                     ETDVWRVIDAWGEDTRRVAFDHAVDLPFRAAIVCRDGEPVQVALCVSHMAADLMGLRVF
                     EAEIARLSAARAEGRAADPPPEFRQPLEQAAFEGSERGLRLLARAGRYWDEQLAGMPAT
                     MFPGAATVPESALPPFHSAVLESRAVALALPVLAERYRAGSSAVLLAVVSVLLGRRGGL
                     RRCGLRLLAANRTEGVLRGTVSNLHQEVPVTVDLSGETVADVVRSARVAAMRAYANGLY
                     DPDQAERVVQGHERARGEHIDLSCCFNDARAVREDRPGASPATPAEIRAAMALSTIEDD
                     DLHEAERFFLVVHDDVPDRVRLVLGAQPQVLSPDDVRAFLHAVERLLVSLVADEMSLAD
                     CAAVAGAAVADGTWRSPRLHA"
     aSDomain        5439..6278
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AB852_RS11900_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.80E-26"
                     /label="AB852_RS11900_Condensation_LCL.1"
                     /locus_tag="AB852_RS11900"
                     /protein_end="299"
                     /protein_start="19"
                     /score="82.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: SHMAADL"
                     /tool="antismash"
                     /translation="PATCAQRHMWNLIQQRMPDSAFYNQIHWAALPPGGTLSDVREVLG
                     ELVGRHESLRTVYQVGGDGGLRQRVIRSGSFTVGTLKAADETDVWRVIDAWGEDTRRVA
                     FDHAVDLPFRAAIVCRDGEPVQVALCVSHMAADLMGLRVFEAEIARLSAARAEGRAADP
                     PPEFRQPLEQAAFEGSERGLRLLARAGRYWDEQLAGMPATMFPGAATVPESALPPFHSA
                     VLESRAVALALPVLAERYRAGSSAVLLAVVSVLLGRRGGLRRCGLRLLAANRTEGVLR"
     CDS_motif       5562..5612
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AB852_RS11900_0001"
                     /evalue="3.80E-05"
                     /label="C2_LCL_024-062"
                     /locus_tag="AB852_RS11900"
                     /protein_end="77"
                     /protein_start="60"
                     /score="16.1"
                     /tool="antismash"
                     /translation="EVLGELVGRHESLRTVY"
     gene            6925..8403
                     /locus_tag="AB852_RS11905"
                     /old_locus_tag="AB852_11650"
     CDS             6925..8403
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /locus_tag="AB852_RS11905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_11650"
                     /product="amidase family protein"
                     /protein_id="WP_245876757.1"
                     /transl_table=11
                     /translation="MDEELCWWPAVRAAKAIAAREVSAREYLAALLAAVERHNPGLGLV
                     VTLSEQAMSDAKEADAATARGDLLPPLHGVPMTVKDCFATAGLRTTGGAAALSGHVPVR
                     DADAVAALRRAGCVVFGKTNLPEFSGDVQAYNELFGTARNPWGTGFSTGGSSGGSAGAV
                     AAGFTPVELGSDVAGSIRVPASHCGVFGHKPSFRTVPTYGHVPPLPFAHTVPDLTVVGP
                     LARTADDLSAVFDAVAGPDSHDAPAWRLELPPARPVRRVAVWFDDPYCPVDAEVRRVLE
                     DVADRLADTGAVVERATPKGIRLDASDRVFRALLAPVAFGRYSVRDAEGIVRGEGVPGA
                     DLGAEHVGQTHRAWADADAARARMQLRWGRFFEEYDAILLPVAPTAAQPHDHRPFAERD
                     ILVNGGRRPYWDQIVWAGLTGVCHLPSTVVPAGRTGRGLPVGVAVAGPYLGDRTTLALA
                     RTLEQALPPLGRPPVLVPLSRSTSGKAEEHARDH"
     gene            8387..9733
                     /locus_tag="AB852_RS11910"
                     /old_locus_tag="AB852_11655"
     CDS             8387..9733
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AB852_RS11910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AB852_11655"
                     /product="hypothetical protein"
                     /protein_id="WP_073786824.1"
                     /transl_table=11
                     /translation="MPETIDLVIPNIVEFNLKRPLLDLDPGVERDLLEHGTNLADQCVF
                     WSDENRVLVLPAGYSPEWLADVHAGLGGSVPPVVSPAPTTGRLVHDLLEDDRALAALYE
                     ATSGARVVRFVSWGAAPELYALASVLRSQGREVLLDIAESRHYWSCEYLESKLSCTDLA
                     THIPGFRVPRGISVASWDQLKGAVTAIAGGGGRAVVKSMYGVGGYGSALARSEGDGMAR
                     FWETMRREPFFGTFPLTVQDYVEHAADCPAVDILVDDDEEPRLEYSMLGVDGLRYRSLT
                     LGPGVLEPALEKRLAELSTTVHAFVRSLGYRGWFSLDCLLGADGELYVTEINARRTGAM
                     HGIALAERWADDSPAVYSHDALPLRLTGIASYTEHIRPVFEELWAKGVRAYPTTVRALA
                     RHRPSLGIIVCAESAAEAERIGGEAHRAVNAAMAEAGTPFSRPTAGSRS"
     misc_feature    complement(10027..10041)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.71"
                     /tool="antismash"
ORIGIN
        1 acccgcttcg agatctcgtg cagattccag gcgggaatcc cgggatagag gtggtgctcg
       61 gcgtggtagt tcgtgttcca gttgaggaag ccgaaccact tgctccgcgt gtagaccgaa
      121 ttggtgttgc gccaggggtt gtcggaccgc tccgcgtagt agtgctcggg catgatggcg
      181 aaattgttca cgatccaggc gacggctgtc ggcatgatgt acagggtgag catcaccatg
      241 ggccacaaga ccgtcaggac ggtcatcgag agaatccaca ccaggagaat ccaggtgtca
      301 cgctggatgg cggagcgggc ccggtcgctg gtgacgaagt agggataccg gtgccacagc
      361 gaggccgccg acatccggag aaaccccagg aagtactcca ggttcagcat ggccatgacg
      421 tactggccca gagtggtgat ggtgccgtcg ggttccgggt cctcgacggt gtgggtattg
      481 cggtggtgat gcatatggaa cacccggtac aaggcactgt tcataccgag gaacgtgccg
      541 gcgacccgtc cggcgagctg attcccgcgg aatgtgttca tgaacgtgcc gtgcgaggcg
      601 cagtgcagca cattgtgcag cgacgcgacc acgaagccgg ccagcaccca gctgagcccg
      661 gtggcccacc aggtgtcgat gacgaaagca cctgtcacgg cgatcgcgta cagcaggacg
      721 tgcaccgacg cgcggcgcat ggccagcgcg ctggagcggc gggtcagttc gcgcagttcg
      781 gcggaggaga agacatcgtt ccggtcgggg tggacgttct cctgcgagat cgtcattcct
      841 gaacctccaa gagggtcgag gcagcgcatg gcggaggcca tggttcaggc cgtgtcctca
      901 gccaggagga cacgagcgtg gtgacccgga cggcgatacc ggccggggcg ggcccggtgc
      961 gtcatcgggg cgcccgggag agggatctgg cgcgactgtc gtccacgaag ccccttgccc
     1021 ggcgggagat cgacggcaaa ggcttcgtcc ataacgggtc acccgtcact cgttccagtc
     1081 acgaccaaaa ggggccttgc ggctgagacg tctcagcatc cgcgtatgcg tcgctttccc
     1141 gtcccgggcc gaccggacag atccggttcg cccgcactgg aatcgacgcc gcgtatcgcg
     1201 acacgatcgc ggaatccgct cgacggtccg cgggcctccc tcacgaaacg cccatcggcg
     1261 ctccgttgcg ccgtgcccac cgtcaaatcg tcctccgaac accgcgtccg cgtttcggcc
     1321 tcactcgcaa actacagatt tcgaaactga aacaacaccc cccatccgcc ttcggcccgc
     1381 ccgccgggcc cccggcgctc ggaccagcgc ctcttgccgg tgaattcctg gcgtcgcgct
     1441 ccaccagggt ccgcttcccg gacccgcttc ccgggtcgca cgatccgggg cgtacgggag
     1501 gctcgaaggc ggcgcgaaca ccgggcggct cgtggtgagg gccgccgggc tgggctgcct
     1561 tggcaaggca tcgaccagcg gaaacaagac agcccccgca agggggcgac tcctcccggg
     1621 cccgacgccc ggcgcttcgg ggtggtcctg aggtcgcgga catcgctccc cgggcacgct
     1681 cgcacgcggc gtcgtcggat gtcgcccgcg ggcgttgaca cacctcgccg ccatggcgca
     1741 gcggattcgg cacgcggaaa ttcgtcaacg aatcactctc gcgtcacccg tgaaaaagac
     1801 gtcgggtcgg taccgacgcc catgcttgct ccacgggccc gggattccga ttcctcggtg
     1861 acccatcggg aacacgcaga tctcgcccga actccttacg tgagctcccg tgcacgcggc
     1921 aagggtattg cccatggcaa agagatcggc gacaggcggc cggatgaatg cggttccgcc
     1981 ggtcatccgc atgtgggcgg ccaccgatga ttccaggcgc tcgatatccg ctctccccgg
     2041 cgcgcgcggt ttcgtatatg gcccgcgtcg tgaacaacgc ccgtgagcaa cgcccgtggg
     2101 cgggcggccc cggccgttgt cccgggtgtc ctccggcggg tcgtcggccg gaggagtgcc
     2161 ggccgacgac cgtcgtggtc agccggtggc cgtccccttg ccggccgtca cggtctcccg
     2221 gagccggtgc cggtcgatct tgcccatgcc ggtgcgtggc agcgcggtga ggacgtgcac
     2281 ccggtgcggc acctcgtagg gcatcaggcg ggcccggcag aaggtgagga catccgcggc
     2341 gtcgttgtcc ggacggagca ccagggccgc gtggacctcc tcctccccgg tccggctggg
     2401 tgctccgaac accagcacct cgctgacccc gggatgttcg gcgaccacgt tctcgacccg
     2461 ccgtggactg accttgcgcc cgccgacgtt cacgaacgcg tccttgcgtc cgacgacgaa
     2521 gacatgaccg tcctcgtcca cctcggtgag gtctccgcag gccaggtagc cgtcctccat
     2581 cgacggtggg ccgccgccgt cctcgtagcc ctcgaacatc gtcgaggtgc gcaccagcag
     2641 atggccgcgt ccgtgggcgg ccgtgcggga gcagccggtc cgcacccgcc agtcgacgcc
     2701 cggggccatg accccggcgc tgtcctccgg ccacggttcg tcgcggtcgt gccggcaggc
     2761 gatcaggccg gtctccgtgc tgccgtacac ctggtgcacg gggactccga gccgggcggc
     2821 ggcggccttc gccgagtcgc tcgtcaaggg gccgccggag gacagggcga cccgtacctt
     2881 cccgcgcggt ccggtccgtg gcagcacggg ggtcatcagc tgatacacca gcggtgtgcc
     2941 caggagtacc gtgctgcccg cgtcgagggc ctccagcacc acaccgggcc ggaaccgggt
     3001 catggtccgc agtccggcgc cggagaccag cgccgtcgcc aacgcgccca ccaggccgaa
     3061 ggaatgcgcc agcggcacca tggccagcac cgtgtcggcg gcggtgatcc ggtggatgtc
     3121 ccggtacagg gcaccgccgg tgagcgtgtt gccgagtgtc tgccgggtga tgcggggttc
     3181 tcccgtgctg ccggaggtga gctgaaggac cgcggtcgcg gggtcctccc gtcgcaccac
     3241 ggtgcccgac ggggcgggga cgctcaccag ctcgtcgaac gtgcggtgcg cgagaccggg
     3301 aacgggcgag atgtcggacg ggccgacgag acccgccggc ggcagccggt ggatgaccga
     3361 gcgggcctcc atcaggtgcg agttctcgcg ttccacgagc agggtgtcgg ccccggcgag
     3421 gaacagcccc agcatggtcg cgacgcaggt cggcgtgttg tccaccagga cgaccaccgg
     3481 tcccgccggg cgttcccagg cggtcgcgtt cgccgccgcg gtcagggcgg catcgtggag
     3541 ttccgcgaag gtcatgtcct gccaagcggc gctgccccgg gtgctgatgg ccaccgcgtc
     3601 cggggcgcgt tcggccccgg cgcgcagaca gccggtcagg gtgggggccg cggaggaacc
     3661 cccggccgga cggggtgact cgccgtgggt catcggcccg accggcttgc cgcgtgatgc
     3721 gaccggacga catcggccag ggcgtccggg ctggcgtcgt cgaagaccgc gagcagcagc
     3781 tcggcgtgga gccggtcggc cgccacggcg tcgtcgggtt cgaagcgctc ggtgagccgc
     3841 gcgatcagct cgacggcacg cagcgagtca ccgccgagga ggaagaagtc gtccgcgggg
     3901 cccaggggcc ggcactccag aaccgttccc atgtcctggg cgatctgcga aggcacgtcg
     3961 ttcgtcgcgg gatcgtcgtt cgtcacagac ccgttcgcct ctctcgatgt gtcgctgggg
     4021 tacaggcgga gctccggtgg cctcgcccct tcgggacggt cctcgggtgt gctccggtcc
     4081 ggggtcctgg tgttgcgcag atacaggtcc gggttctccg cgaagcagcg ttgcgcctct
     4141 tcgtaccttt cgggcgttcc gatgtcatgc catcggtcgc tgaaggaata ggcgtggacc
     4201 cggcggccgc tttccatcat cgatcccacc aggtcgggca tgtcgacgac tttccccggg
     4261 gtaatcgctt cgagtacggc cggatcggcc acatagattc ccgcggagat atcgagatgc
     4321 agcgtcggct tctcccatat cccggccacc tggtcgcccc ggatgtcgag gacgccgaaa
     4381 cctatcggca tggggcggcg gaacgacgcg acggtcagca ctcccccgct gtccttgtgc
     4441 acccggtaga gctcggcgaa gtcgagcgcg gtcaggacgt cgccgttcat gaccagcgcc
     4501 ggctcgttga agtccgcgac ctgggccagt ggcgccgcgg tccccagggg ccgcgggtcg
     4561 gtgctgtagt cgatgcggac gccgagcgtc gccccgtcgc cgaactcggc cctgatctgg
     4621 tccgccaagt gggagacaca caaggtgata tggctgaatc cggcgcctcg gagcctgcgg
     4681 atgatgactt cgaggatgga gtaccgactc agctggagca gacctttggg ccggttttcg
     4741 gtgtatggcc ggaggcgtgt ccccttccct ccggtcagga tgatcgctcg cacgagagcc
     4801 tccctgtgcc gccatcggca tcgcggcggg atcaagcctc agaggcggga cccggttggg
     4861 ggaaggctca aacggccgcc ccggcaggcc tagccaggtg gccatgcccg tagagtccgt
     4921 ggatttactc gtaccgcatt ccatagactc gacgccgacc ctcgcagaaa atccaaatcc
     4981 tgggcaggtg aaccttgctg gtgcgctcgg ttgaagaagt cgtgggaagc gaatgcgacg
     5041 tggactgggg taacgggacg agcagacgac ttctcgttca ggcggataat ttggggttca
     5101 gcctgaccga gacatatgta cagcccggca gcgagtcgta tctgcggtac gacaaccacc
     5161 aagaggtctg ctattgcgtg agcggatccg gttccgtgga gacggaggac ggcgttttcg
     5221 agataaagcc cggaacgctg tacgcccccg gtgtcggtga accgcatgtg ctgcgctcgc
     5281 agcacggtat gacgctgatg tgcgtcttct cgccggcctt gcacggatcg gagacacacc
     5341 tgctcacgcc cggtgtgcat tcgagttact aggggacggc cgtggctgaa acgatccagt
     5401 gtgaagtggc cttcaccggc gggcggttcg ccgccggtcc ggcgacatgc gcgcaacggc
     5461 atatgtggaa tctgatccag cagcggatgc cggactccgc gttctacaac cagatccact
     5521 gggccgctct gccgcccggc ggcacgctgt ccgatgtgcg cgaggtgctg ggcgaactgg
     5581 tcggccgcca tgagtcgttg cgtacggtct accaggtcgg cggggacggc gggctgcgcc
     5641 agcgggtgat ccggtcgggg tccttcacgg tcgggaccct gaaggccgcc gacgagacgg
     5701 atgtgtggcg tgtcatcgac gcgtggggcg aggacacccg tcgcgtcgcg ttcgaccacg
     5761 ccgtggatct gccgttccgg gccgcgatcg tgtgccggga cggcgaaccg gtccaggtcg
     5821 ccctctgtgt ttcccatatg gcggcggacc tgatggggct gcgggttttc gaagcggaga
     5881 tcgcacggct gtcggccgcc cgcgcggagg gccgcgccgc cgatccgccg cccgagttcc
     5941 ggcagcccct ggaacaggcg gcgttcgagg gctccgagcg gggcctgcgt ctgctggcac
     6001 gtgccggtcg ctactgggac gaacagctgg cggggatgcc cgcgacgatg ttccccggtg
     6061 cggccacggt gccggaatcc gctctgccgc ccttccacag cgctgtcctg gagtcacggg
     6121 cggtcgcgct ggcgctgccg gtgctcgccg agcgctaccg ggccggcagc tcggcggtgc
     6181 tgctcgccgt ggtgtcggtg ctcctggggc ggcgcggcgg tcttcggcgg tgcgggctgc
     6241 ggctcctcgc ggccaaccgc accgagggag tactgcgcgg cacggtgtcc aatctgcatc
     6301 aggaggtgcc cgtgaccgtc gacctgagcg gggagaccgt cgccgatgtg gttcgctcgg
     6361 cgcgtgtcgc ggcgatgcgg gcgtacgcca acggtctgta cgaccccgac caggccgagc
     6421 gtgtcgtcca ggggcacgag cgcgcacgcg gcgagcacat cgatctgtcg tgctgcttca
     6481 acgacgcacg ggccgtccgg gaggaccggc cgggggcgag tcccgcgaca ccggcggaga
     6541 tccgtgcggc catggccttg agcacgatcg aggacgacga cctccacgag gcggagcggt
     6601 tcttcctggt ggtccacgac gacgtgcccg accgcgtccg gctcgtcctc ggcgcgcagc
     6661 cccaggtgct gtccccggac gacgtacggg ccttcctcca tgccgtcgaa cggctgctcg
     6721 tgtccctcgt cgcggacgag atgtcgctcg cggactgcgc cgcggtcgcc ggggcggccg
     6781 tggcggacgg cacctggcgc tcacctcgtc tccatgcgta gcgggagccg gccggctccc
     6841 cctcgggagc cgtcgggggc ggaccggccg gtcccagggc ccgggggcgg ggccccgacg
     6901 acaagaggtg gtggcgcgtt gggcgtggac gaggagctgt gctggtggcc ggcggtcaga
     6961 gccgccaagg cgatcgccgc gcgggaggtc tcggcgcggg agtaccttgc ggcgctcctc
     7021 gccgcggtcg aacggcacaa ccccgggctg gggctggtcg tgaccctgag cgagcaggcg
     7081 atgtccgacg cgaaggaggc ggacgcggcg accgcgcgcg gtgatctgct gccgcccctg
     7141 cacggtgtcc ccatgacggt caaggactgc ttcgcgaccg ccggtctgcg taccaccggc
     7201 ggcgccgccg cgttgtccgg ccatgtgccc gtccgtgacg ccgacgcggt ggcggcgctg
     7261 cgccgggcgg gatgtgtcgt cttcggcaag acgaatctgc cagagttctc cggcgatgtg
     7321 caggcgtaca acgagctgtt cggcacggcg cgcaacccat gggggaccgg cttctccacg
     7381 ggaggttcct caggggggtc ggcgggggcg gtcgccgccg ggttcacccc ggtcgagctg
     7441 ggctccgacg tcgccggttc gatccgtgtt cccgcgtcgc actgcggtgt cttcgggcac
     7501 aaaccgagtt tccgtacggt gccgacgtac gggcatgtcc cgccgctgcc gttcgcccat
     7561 acggtccccg atctgaccgt cgtgggtccg ctcgcccgta ccgcggacga tctgagcgcc
     7621 gtgttcgacg ccgtcgcggg gcccgactcc catgacgcgc ccgcctggcg gctcgaactg
     7681 ccgccggccc gcccggtcag gagggtggcg gtctggttcg acgacccgta ctgcccggtg
     7741 gacgcggagg tccgccgggt gctcgaagac gtggcggacc ggttggccga cacgggtgcc
     7801 gtggtggagc gggccacccc gaagggcatc cggctggacg cgagcgaccg ggtcttccgc
     7861 gcgctgctcg cgccggtcgc gttcggccgt tactcggtgc gcgacgccga ggggatcgtc
     7921 cgcggcgaag gggtcccggg cgcggatctc ggtgccgagc acgtcgggca gacccatcgg
     7981 gcctgggcgg acgccgatgc cgcccgggcc aggatgcagc tccgctgggg gcggttcttc
     8041 gaggagtacg acgcgatcct gcttccggtg gccccgaccg cggcgcagcc gcacgaccac
     8101 cgtcccttcg ccgaacggga catcctggtg aacggcggtc gccgtcccta ctgggaccag
     8161 atcgtctggg ccgggctgac cggtgtgtgc catctgccct cgacggtggt ccccgccggg
     8221 cggaccggcc gcggactgcc ggtcggtgtg gcggtcgccg ggccgtatct cggtgaccgc
     8281 accacgctcg cgctcgccag gaccctggaa caggcgctgc cgcccctcgg ccggcccccg
     8341 gtcctcgtac ccctctcgcg cagtacgtct gggaaggcgg aagagcatgc ccgagaccat
     8401 tgacctggtc atcccgaaca tcgtcgagtt caatctgaaa cgcccgctcc tggatctcga
     8461 cccgggggtg gaacgcgacc tcctggagca cggtacgaac ctcgccgacc agtgcgtctt
     8521 ctggagcgac gagaaccgtg tgctcgtgct cccggccggc tactcgccgg agtggctcgc
     8581 cgatgtccac gccggtctcg gcggctccgt tcccccggtc gtctcaccgg cgccgaccac
     8641 cggacggctg gtccacgacc tcctggagga cgaccgggca ctcgccgcgc tgtacgaggc
     8701 gaccagcgga gcgcgcgtgg tgcggttcgt gtcgtggggg gcggcacccg agctgtacgc
     8761 gctggcctcg gtgctccgca gccaggggcg ggaagtcctg ctggacatcg cagaatcccg
     8821 gcactactgg tcgtgcgagt acctggaatc caagctgagc tgcaccgatc tcgccacgca
     8881 catcccgggg ttccgggtgc cgcggggtat ctcggtcgcc agttgggacc agctcaaggg
     8941 cgcggtcacg gcgatcgcgg gcggcggcgg gagggccgtg gtgaagagca tgtacggcgt
     9001 cggcggctac ggttccgccc tcgcgcggag cgagggcgac gggatggccc gcttctggga
     9061 gacgatgagg cgcgaaccct tcttcggtac cttcccgctg accgtgcagg actacgtcga
     9121 gcacgcggcc gactgtccgg cggtggacat cctcgtcgat gacgacgagg agccgcggct
     9181 ggagtacagc atgctcggcg tcgacggact gcggtaccgc agtctgaccc tcggccccgg
     9241 tgtcctcgaa cccgctctgg agaaacggct cgccgagctg agcaccaccg tccacgcgtt
     9301 cgtccggtcc ctcgggtacc ggggctggtt ctcgctcgac tgcctgctcg gtgccgacgg
     9361 agagctctac gtcaccgaga tcaacgcccg tcgtacgggt gccatgcacg ggatcgcgct
     9421 cgcggagcgc tgggccgacg acagcccggc cgtctacagc catgacgccc tgcccctgcg
     9481 gctcaccggt atcgcctcgt acacggaaca catccgcccg gtgttcgagg aactgtgggc
     9541 gaagggcgtc cgcgcctatc cgacgaccgt ccgcgcgctg gcccggcacc ggccttcgct
     9601 gggcatcatc gtgtgcgccg agagcgcggc ggaggcggag cgcatcggcg gcgaggcgca
     9661 ccgggccgtc aacgccgcca tggccgaggc cggaaccccg ttcagcaggc cgaccgcggg
     9721 cagccgctcg tagcggcggc gaccggggcg gcggcagcgg ctgggggggg cggctcgtgg
     9781 tcatcgcggc tcgtgggcta cggctctcgt gggctacggc tctcgcgtgg tcgaggggtc
     9841 ccggcctgac ggcggtgccc cagatcgtcg cgggacgggt tccctgaccc ggcggcgccg
     9901 gccggggtgt gggaggcgat cggccgggta cccgatcgtc tgtcacacgt tcggaacgac
     9961 gacgtaaagg gagcgatgat gtcgaagatc gaggaatccg tcgaggtcgc cgtcccggtg
    10021 agcacggcct acaaccagtg gacgcagttc gaggagttcc cgcggttcat ggacgcggtg
    10081 gaacggatcg agcagctcac gtccacgctg acgcactggg tcaccaaggt ggacggggtg
    10141 agccgggagt tcgacgcgga gatcaccgag cagcggcccg acgagcgggt cgcctggacc
    10201 acggtggcgg gtgagatccg tcaggccggg gtggtcacct tccaccggct cgacgacacc
    10261 cggaccaagg tcatgctcca gctcgaccac gatccgcagg gcatcaccga caccgtcggc
    10321 gacaagctcg gcttcgtccg ccgccaggcc aagggcgatc tgaagaactt ca
//
