LOCUS       NZ_LFBV01000001        10438 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces uncialis strain DCA2648 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_LFBV01000001
VERSION     NZ_LFBV01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces uncialis
  ORGANISM  Streptomyces uncialis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2018837)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Cloning and characterization of the uncialamcin biosynthetic gene
            cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2018837)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33410, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-MAY-2015
            Assembly Method                   :: CLC Genomics Workbench v. 8.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905345.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,456
            CDSs (total)                      :: 7,371
            Genes (coding)                    :: 7,214
            CDSs (with protein)               :: 7,214
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 42 of 157
            Pseudo Genes (incomplete)         :: 143 of 157
            Pseudo Genes (internal stop)      :: 7 of 157
            Pseudo Genes (multiple problems)  :: 33 of 157
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 641560
            Orig. end                         :: 651998
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LFBV01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10438
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="4"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10438
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10438
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5438](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5438
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(933..946)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.51"
                     /tool="antismash"
     gene            complement(1093..2325)
                     /locus_tag="AB852_RS02745"
                     /old_locus_tag="AB852_02695"
     CDS             complement(1093..2325)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980464.1"
                     /locus_tag="AB852_RS02745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02695"
                     /product="DUF3344 domain-containing protein"
                     /protein_id="WP_178390929.1"
                     /transl_table=11
                     /translation="MRNSLAPVLRRLLLPPRTTVCVLSLCAVAAGGFAQAPPVHGADPD
                     DGRPGGVVAPALPAGVPAARPAAPPRIAPYASEAASVPFAQRFHAVQHGGITRAANTSI
                     TCRGPAPTPSAVSCADARDGRAAANDDFDMFYTDVDSDPNTYNSSRGELRAPAGAKVSY
                     ARLYWGGNLLVGEQKPARDNGRVLVAEPGGSYKEVLADSVVGHRIAGGADAFQASADVT
                     GLVRSGGTGLWTVAQVNVAMGHSQAGAWGGWTLVVAYERASEPLRQLSLWDGFQYLGES
                     PNDAALRLDGAAAPAGARGQAGFVVYNGDTGTSGDALTLSVGRRAAATLSGPGNPAGDL
                     FNSTVTEPGTSQPRRDPAHTGTLGYDSDVLELGEAVADGGGRLDLRLTAERDAAWAGVL
                     FLAVETDTDAE"
     misc_feature    complement(2348..2363)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.44"
                     /tool="antismash"
     misc_feature    2653..2669
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.94"
                     /tool="antismash"
     gene            2669..2908
                     /locus_tag="AB852_RS02750"
                     /old_locus_tag="AB852_02700"
     CDS             2669..2908
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="AB852_RS02750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02700"
                     /product="chaplin"
                     /protein_id="WP_073783191.1"
                     /transl_table=11
                     /translation="MSRTAKALVLSTVAAAAVAGAAGVAAADAGAQGAAVGSPGVLSGN
                     VVQVPVHIPVNVCGNTIDVIGILNPAFGNVCVND"
     misc_feature    complement(3097..3110)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.12"
                     /tool="antismash"
     misc_feature    complement(3125..3138)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.65"
                     /tool="antismash"
     misc_feature    3127..3140
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.63"
                     /tool="antismash"
     misc_feature    3127..3140
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.36"
                     /tool="antismash"
     misc_feature    3128..3141
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.1"
                     /tool="antismash"
     misc_feature    3128..3142
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.3"
                     /tool="antismash"
     misc_feature    3129..3142
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.56"
                     /tool="antismash"
     misc_feature    complement(3130..3143)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.29"
                     /tool="antismash"
     misc_feature    complement(3131..3144)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.4"
                     /tool="antismash"
     gene            3314..3595
                     /locus_tag="AB852_RS02755"
                     /old_locus_tag="AB852_02705"
     CDS             3314..3595
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529518.1"
                     /locus_tag="AB852_RS02755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02705"
                     /product="hypothetical protein"
                     /protein_id="WP_073783193.1"
                     /transl_table=11
                     /translation="MKPLKAAALVAGSFALAGAAAPAFASDLTPTSLNGALDTLTTQQT
                     LDAQPLHSNAIDTERKGSLLYGLNETTDQLNAGGAQTGPLLGGLPVGK"
     gene            3764..4267
                     /locus_tag="AB852_RS02760"
                     /old_locus_tag="AB852_02710"
     CDS             3764..4267
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746418.1"
                     /locus_tag="AB852_RS02760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02710"
                     /product="DUF5949 family protein"
                     /protein_id="WP_073783195.1"
                     /transl_table=11
                     /translation="MTSTPTDKRPLHPAELGTLAVMAWSGEHPDERGDLAFLLAYSLGD
                     GPGGAEGTSAAVRRLLENTGLPPGGPVIDARTRPSFPLTLLVEAGQAVVNMPHLNAQCV
                     VPREWLAAVEERGHAYFLFATVPWPEGTPGVEMTERTLSAFAGDEGVLATAAHCLLPAR
                     SLRS"
     misc_feature    complement(4962..4978)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.23"
                     /tool="antismash"
     gene            5001..5438
                     /locus_tag="AB852_RS02765"
                     /old_locus_tag="AB852_02715"
     CDS             5001..5438
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059086.1"
                     /locus_tag="AB852_RS02765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02715"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_245876586.1"
                     /sec_met_domain="melC (E-value: 1.5e-46, bitscore: 154.7,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGTITAHRTRRTLLRTLFTAAVATTTATALAPLLAATREPHRPR
                     APRAPAELLFEETYRGRRIEGRGPGTPTAARTRPVGHHGSGGPRVTIDGRPLPLMRRAD
                     GSYLSVVDHYVSYPTPLAAARAAVDELGPARLAPGTGARNV"
     gene            5456..6322
                     /locus_tag="AB852_RS02770"
                     /old_locus_tag="AB852_02720"
     CDS             5456..6322
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="AB852_RS02770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02720"
                     /product="tyrosinase family protein"
                     /protein_id="WP_073783199.1"
                     /transl_table=11
                     /translation="MAHTRKNVTALTRAERKRFVDAVLAMKRRGRYDEFVRLHIEHYVA
                     DGEGDRRVAHMAPSFLPWHRRFLLEFERALQREVPGVTVPYWDWTKDRSPRALPWTDDL
                     LGGTGHGPDRRVESGPFARANGWVVRENTTDDDHLARDLGRPSRPFPLPSKEELAYAME
                     QSAYDVAPWNSTVTSGFRNTLEGWTSARTGDDRWRNHNRVHRWVGGTMLGGASVNDPVF
                     WLHHSFVDLLWSRWRASRPDGAYQPAEPPGIGSVQRGRVVARHERMTPWEETPAAMEDH
                     SRIYRYR"
     gene            complement(6404..6625)
                     /locus_tag="AB852_RS02775"
                     /old_locus_tag="AB852_02725"
     CDS             complement(6404..6625)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449724.1"
                     /locus_tag="AB852_RS02775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02725"
                     /product="chaplin"
                     /protein_id="WP_281257706.1"
                     /transl_table=11
                     /translation="MLGGAGMAAASSGAEAAAIGSPGVLSGNIVQVPVHIPVNVCGNTI
                     DVVGILNPAFGNACVNGSAPQAQSGYGA"
     gene            complement(6825..8813)
                     /locus_tag="AB852_RS02780"
                     /old_locus_tag="AB852_02730"
     CDS             complement(6825..8813)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1214:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010071480.1"
                     /locus_tag="AB852_RS02780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02730"
                     /product="ABC transporter permease"
                     /protein_id="WP_073783201.1"
                     /transl_table=11
                     /translation="MKTRPARAPGTTPPARPPLALALPAALAVAFLMLPLLGILARTPW
                     GDLAEHLTAPGAVTALRLSLVVSGWALALALLLGVPLAWLLARVPFRGKAFVRTLVLLP
                     MVLPPTVGGVALLLGFGRRGLLGPWLEDWFGITLPFHTAGAVVAAAFVSMPFLVISLEG
                     TLAALSTRYEEAAASLGASPFRVFRTVTLPMVAPGLAAGAALTWARALGEFGATITFAG
                     NLPGTTQTLPLHVYLLLQDDPEAATSVSLLLLAIAMAVLIALRGRWTTTPATAAPRADR
                     DPAPEPRTAPEPGPSAPDPAQDPAPVPDSVPADRAPRATALDARLTGFTELALTAEGGT
                     TLAVVGPNGAGKTTLLRALLGLPTRSHAALRLGATDLTDAPPHRRSTAWVPQSPALFPH
                     LTALANTAYGPRAQGVPRAEARAAAQHWLDRLEVGHLAGRRPGQLSGGQAGRVALARAL
                     AARPRLLLLDEPLAALDQTTRARVRTTLRRHLDDFDGVCLIVTHDPVEAVSLADRVLVL
                     EDGRTVQDAPPAEVSRHPRSPWVARMLGHNAWAGTATADGLRTPDGTLVIAAEPLPPGA
                     GALAVIAPEAVSLHRDRPTGSPRNVWSGTVGELTAVGSRLRVLVTSATAPDLVAEITPG
                     AAADLGLREGSEVFTSVKATEVVLVLV"
     gene            complement(8872..9654)
                     /gene="modA"
                     /locus_tag="AB852_RS02785"
                     /old_locus_tag="AB852_02735"
     CDS             complement(8872..9654)
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0030973 - molybdate ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="modA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523563.1"
                     /locus_tag="AB852_RS02785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02735"
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_073783203.1"
                     /transl_table=11
                     /translation="MSRSPARTAATALAATALLIPLLAACGDDEEKKDSGAQDSAKLTV
                     LAAASLTDVFGTVKSAYEDAHPGTTLTFSFAGSQELAAQVRQGAPADAIVTADTRTMDG
                     LKADTGTPSVIARNRLVIATREGNPDKVDGLKDLADPKLKVVLAAPEVPVGRYSRQVLD
                     AQKIDVEPVSEEPNVRAVLSKVELGEADAGIVYKTDAETATDKVDAITVPDDQNAIAQY
                     PAAVIKGGKNVAAAQTFVEWLSTPAAQKILQDAGFQKP"
     gene            complement(9706..10098)
                     /locus_tag="AB852_RS02790"
                     /old_locus_tag="AB852_02740"
     CDS             complement(9706..10098)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551052.1"
                     /locus_tag="AB852_RS02790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_02740"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_073783205.1"
                     /transl_table=11
                     /translation="MPSYTIGQAARLLGVSADTARRWADAGRIATHRDEGGRRLVDGVD
                     LAAFSVELAQGEGENDVSYTSARNAFPGIVTAVKLGDVAAQVEIQAGPHRLVSLLTREA
                     VEELGLEVGVQATARVKSTNVHIDRS"
     misc_feature    complement(10328..10341)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
ORIGIN
        1 gcgcgtgcca gcgcggcggg atccccggga ggcacgagac agtacgcgcg atggccgggc
       61 gcgaggctct cgcgcgcccc ggcgacatcg gtcagcagca cgggccgccc gcacgccatc
      121 gcctccagcg gggccagcgc catcccctcc caccgggacg ggaggacgac gaggtcggcg
      181 gcccggtacc agggggcggg gtcctcggcg gccccggcga agatcacccc ttcgggtgag
      241 ccggcgcgca gccgttcccc gtccggcccg tcacccacga gggccagccg cgccccgggg
      301 acccgtcgta cgacctcggg ccaggcccgc agcagcacgt cctgcccctt ctggcggcag
      361 agccgggcga cgcatacgac gaggggggcg cgcgggtcgg tcccggcgag cgcgggcggc
      421 gcgtcatggg gggcgcggcg cgcgcggggc agtacgtcgt gcgcgggggg ctccggccgg
      481 aaccggtcgg tgtccacgcc gttggggacg accgtccagt ggccgcgtac cccggcggcc
      541 tcgcccgtgg cgcgttccgc ctcgctgacg cacacgatcc gcgaggccca gcgtgccgcg
      601 ccgacctccc agcggcgggc gagccgggcg gcggtcccct cgacggcctc gaacgaccag
      661 gcgtgcggct ggaacacggt cggtacccgg ccccgcagcg cgagccgggc ggcgagtccg
      721 gccttggcgc tgtgcgcgtg gacgagtccg ggacgggtgc gggcgatcag ccgggccagt
      781 gcgcggatct cggagggcag cgcggtgccg ggggagcggg tggcgtccca gcgcagccag
      841 tcggcgcccg cggcggtcac ccatccggcg agcggcccct gttcggggca ggcgacgagg
      901 aggcgcggcg cgtcgggggc cgtgaggtgc tgctggatca ggtccagcac cacccggggc
      961 accccgcccg tcacgggctg cacgacgtgc agcacccggg gcggcgcgtc ggccggggcg
     1021 cggccgggcc gtggcggccg cgcggcccgc gccgcttcgg cgcgggtgtc cggccggccc
     1081 gtcggggacg ggttactcgg catcggtgtc ggtctcgacc gcgaggaaca ggacccccgc
     1141 ccaggcggcg tcccgttcgg cggtcagccg cagatcgagc cgcccgccgc cgtcggcgac
     1201 cgcctcgccg agctccagca cgtcggagtc gtacccgagg gtgccggtgt gggcggggtc
     1261 gcggcgcggc tgcgaggtgc cgggctcggt gacggtggag ttgaagaggt caccggccgg
     1321 gttgccgggg ccggacaggg tggccgcggc gcgcctgccg acggagaggg tcagcgcgtc
     1381 gccgctggtg ccggtgtcgc cgttgtagac gacgaacccg gcctggccgc gggcccccgc
     1441 cggggcggcg gcgccgtcga gccgcagcgc cgcgtcgttc gggctctcgc ccaggtactg
     1501 gaagccgtcc cacaggctga gctgccgcag cggctcgctc gcccgctcgt acgccacgac
     1561 cagcgtccag ccgccccacg cgcccgcctg cgagtgcccc atggccacat tgacctgggc
     1621 gaccgtccac agtcccgtgc cgccggagcg caccaggccg gtgacgtccg cggaggcctg
     1681 gaaggcgtcc gcgccgcccg cgatccggtg cccgacgaca ctgtccgcga ggacctcctt
     1741 gtacgagccg cccggctcgg cgacgagcac ccgcccgttg tcccgggccg gcttctgctc
     1801 acccaccagc agattgccgc cccagtacag ccgcgcgtac gagaccttcg ccccggcggg
     1861 cgcgcgcagt tcgccgcggc tggagttgta ggtgttcggg tcggagtcga cgtcggtgta
     1921 gaacatgtcg aagtcgtcgt tggccgcggc acgtccgtcc cgcgcgtcgg cgcaggacac
     1981 ggcggacggg gtgggcgcgg gtccgcggca ggtgatcgac gtgttggcgg cccgggtgat
     2041 gccgccgtgc tgcacggcgt ggaaccgctg ggcgaacggc acgctcgccg cctccgaggc
     2101 gtacggcgcg atgcgcgggg gagcggcggg ccgcgcggcc ggtaccccgg cggggagcgc
     2161 gggggcgacg acgccgcccg gcctgccgtc gtcggggtcc gccccgtgga cgggcggggc
     2221 ctgggcgaac ccgcccgccg ccaccgcgca gagcgaaagg acacacaccg tggtacgcgg
     2281 cggcagcagc agccgtctga ggaccggggc gagggaattg cgcatgaccg gcattgccct
     2341 ttcggggatg taaagatcct gaacccgtcg gaaggcagta tgaccatcac acatcgacga
     2401 agcaggactc cccaccgccg attcggccgt ccgtggagag ggaagccact ctaaccgccg
     2461 agcttatttg ctgctgatac cgcggaagtc tgtgtgtcgg attaatgggt atccgcgatt
     2521 tcgttcggca ctctcgcggg tgttgttgag cgggagcgcc gagagatcac cctttcggat
     2581 gcggaacccg ccggagggtc ggacgttggt ccatgggccg ggcatcctcg cctggtgcgt
     2641 cacccaacga tctaggagca ttacttccat gtcgcgtacc gcgaaggctc tcgtcctgtc
     2701 caccgtcgcc gccgccgctg tcgcgggcgc cgcgggcgtc gccgccgccg acgccggggc
     2761 ccagggcgcg gccgtgggct cccccggtgt gctctcgggc aacgtcgtcc aggtcccggt
     2821 ccacatcccc gtgaacgtct gcggcaacac catcgacgtc atcggcatcc tcaaccccgc
     2881 gttcggcaac gtctgcgtga acgactgagg cccgaccttt ccgcgagccg cccgccccct
     2941 tccggggccg ggcggctcgt tcgtttgtac ggatgttccg ccgacgcaat tgcagggctc
     3001 cggaggttgt ccggcggtgg ccggtgcggg cgatttccgg gctcttcgac gggcttcctg
     3061 gtggaattcc gtacgggtga tcggattgcg ctcatgtatt cgtgagaaaa ggtccttctc
     3121 tcgctctatt tttcatataa aaccatcgga acgggtcctg ccctgcctcg ggtgaatggt
     3181 ggggaaacac cttcgggtgg gggttgtgtg caattcgctc tccggcggtt tccggcacgg
     3241 ccggaggtcg ttagtccacg cggtaggacg cattcccact ggcggccccc gggccgccga
     3301 agaaaggaac acgatgaagc ccctcaaggc cgccgctctc gttgccgggt ccttcgccct
     3361 ggccggtgcc gccgcaccgg cgttcgcctc cgacctcacg cccaccagcc tgaacggcgc
     3421 gctggacacc ctcaccacgc agcagacgct cgacgcgcag ccgctgcact ccaacgcgat
     3481 cgacacggag cggaagggct ccctcctgta cggcctcaac gagaccaccg accagctgaa
     3541 cgcgggcggc gcccagaccg gtccgctgct cggcgggctc cccgtcggca agtagccacc
     3601 gcggtccgcg cgcggcggtg cgtgagggga ccggcgcgcg gaccccaccc gcgctcgtcg
     3661 cacgccacgc gcgcgacccc caacgtgtga tccccgtacg cgtgtccctc ggacgagtga
     3721 cacgaatatc cgggtcatcc tcatatgtcg ctacggtgcc ggggtgacct caacccccac
     3781 tgacaagcgc cccctccacc ccgctgagct gggcaccctc gccgtcatgg cctggagcgg
     3841 cgagcacccc gacgaacggg gcgacctcgc cttcctcctc gcctactccc tcggtgacgg
     3901 gcccggcggc gcggagggca cctcggccgc ggtgcgccgg ctgctggaga acaccgggct
     3961 gccccccggc gggcccgtca tcgacgcgcg gacccggccc agttttccgc tgaccctgct
     4021 ggtcgaggcg gggcaggccg tcgtcaacat gcctcatctg aacgcgcagt gcgtggtgcc
     4081 gcgggagtgg ctcgcggcgg tggaggagcg ggggcacgcg tacttcctct tcgcgacggt
     4141 gccttggccc gaggggactc ccggggtcga gatgacggag cggacgctgt ccgctttcgc
     4201 gggggacgag ggggtgttgg cgactgccgc gcactgtctg ctgccggcgc ggagcctgcg
     4261 gagctgaagc tccgttcggg tgcgcggttc ttccctgcgg gtcgccttcg cttgcgcttc
     4321 ccaggtctgt ccgggtcggc gcactcgttc ctgtgccgtc gctcggtggg tgcgcagttc
     4381 cccgcgcccc tggatgctgc cccctcgctg tcgttcgtcg ggtgcggggc cgccctcgtc
     4441 ttttgcgcag ttccccgcgc ccctatcggg gcgccatctg cttgttcttc gggtcggggc
     4501 cggtcgggat tctccgtcct cgctccaacg cgctcggtcg gacatccccc ttgccctgct
     4561 gaagagcatc ggagtctgcg ggcagagatt cccgcccacc ccctcccgca gtcatgcgac
     4621 tgcacgagga ggatggtcca accccgtccc cgcagacaga gaacagcctc aggagggcgc
     4681 cccaaagggg cgcggggaac tgcgcgaaga cgaggaccgg cccgcacccg acgaacaacg
     4741 gaccggggca gcatccaggg gcgcggggaa ctgcgcagcc acggacgtac ccgtacgggg
     4801 acaagtgcgt tcgggtgggg aacctcgggg gcgcgagcgg aggcgacccg cacagggaca
     4861 agcgccaggt acaggcaacc cgggggcgcg ggcggaggcg gggtttgggc tggatggggg
     4921 agtgggtggg ggagggtgac ctctgggggg aacggacgtt gaggtaggga aagtacgacc
     4981 gtatggaagg gaggtgcccg gtgcccggga cgatcaccgc acaccgcacc cgcaggaccc
     5041 tgctgcgcac cctgttcacc gcggccgtgg cgaccaccac cgccaccgcc ctcgcccccc
     5101 tcctcgccgc gacccgcgaa ccccaccgcc cccgcgcgcc ccgcgccccc gccgagctcc
     5161 tgttcgagga gacctaccgg ggccgccgga tcgaggggcg gggccccggg acaccgaccg
     5221 ccgcccggac ccgcccggtg gggcaccacg ggtccggcgg gccgcgggtc accatcgacg
     5281 ggcggcccct gccgctgatg cgccgggccg acggcagtta tctgagcgtg gtcgaccact
     5341 acgtctcgta cccgaccccc ctcgccgcgg cccgcgccgc cgtggacgag ctgggccccg
     5401 cccgtctcgc gcccggcacc ggcgcgcgga acgtctgagc cgggggaggg gacccatggc
     5461 gcacacccgc aagaacgtca ccgcgctcac ccgcgcggag cgcaaacgtt tcgtggacgc
     5521 cgtcctcgcg atgaaacgcc ggggccgtta cgacgagttc gtccggctgc acatcgagca
     5581 ctacgtcgcc gacggcgagg gcgaccgccg ggtcgcgcac atggcgccgt cgttcctgcc
     5641 gtggcaccgc cggttcctgc tggagttcga gcgcgcgctc cagcgggagg tgcccggggt
     5701 caccgtcccg tactgggact ggacgaagga ccgttcgccc cgcgcgctcc cctggaccga
     5761 cgacctgctc ggcgggaccg ggcacgggcc cgaccggcgg gtggagagcg gcccgttcgc
     5821 gcgggccaac ggctgggtgg tgcgggagaa caccacggac gacgaccatc tcgcccggga
     5881 cctgggccgc ccctcgcggc cgttcccgct gccgtcgaag gaggagctcg cgtacgccat
     5941 ggagcagtcg gcgtacgacg tggcgccgtg gaactccacc gtcacgtccg ggttccgcaa
     6001 cacgctggag ggctggacct cggcgcgtac cggggacgac cgctggcgca accacaaccg
     6061 ggtgcaccgc tgggtcggcg ggacgatgct cggcggggcg tcggtgaacg acccggtgtt
     6121 ctggctgcac cactcgttcg tggatctgct gtggtcccgg tggcgggcgt cccggccgga
     6181 cggggcgtac cagcccgcgg agccgccggg gatcgggtcg gtccagcggg gccgggtggt
     6241 cgcccggcat gagcggatga cgccgtggga ggagacaccc gcggccatgg aggaccacag
     6301 ccggatctac cggtaccggt gagccggtac gcgaaacccc cgtcagcagg tgaaggccgc
     6361 tgacgggggc gctcgtacga ccggggtgcc gtacgcgggg gaatcaggcg ccgtagccgg
     6421 actgggcctg gggggcgctg ccgttgacgc aggcgttgcc gaacgcgggg ttcaggatgc
     6481 cgacgacgtc gatggtgttg ccgcagacgt tgaccgggat gtgcaccggg acctggacga
     6541 tgttgccgga caggacgccc ggcgagccga tggccgcggc ctccgcgccc gagctggcgg
     6601 cggccatgcc ggcgccgccg agcatgacgg cgcccgtgcc gacggccagg acggctgcct
     6661 tcgcgatgcg agacatcacg tactccttca gttggttgtg ctgtggttcg tgtgcggcgc
     6721 cgagggacgg cgtccgcact cccccttcaa cgtcgggagg cggcccgggt cacggtttgt
     6781 gggcccttgg gcaggggaag agcgccgagc gggtggcggg gccgtcagac gaggacgagg
     6841 acgacctcgg tggccttgac gctggtgaag acctccgagc cctcgcgcag cccgagatcg
     6901 gcggcggccc cgggggtgat ctccgcgacc aggtcggggg cggtggcgga ggtgacgagg
     6961 acccgcagtc tgcttcccac ggcggtgagt tcgccgaccg tgccggacca gacgttgcgg
     7021 gggctgccgg tggggcggtc ccggtgcagg gacaccgcct ccggggcgat caccgcgagg
     7081 gccccggcgc cggggggcag gggttcggcg gcgatgacga gggtgccgtc cggggtgcgc
     7141 agcccgtcgg cggtggccgt gccggcccag gcgttgtgcc cgagcatccg ggccacccac
     7201 ggggagcgcg gatggcggct cacctcggcg ggcggggcgt cctgcacggt acggccgtcc
     7261 tccaggacga ggacccggtc cgcgagcgac acggcctcca ccgggtcgtg ggtgacgatc
     7321 agacagacgc cgtcgaagtc gtccagatgg cggcgcaggg tggtgcggac ccgggcgcgg
     7381 gtcgtctggt cgagggcggc gaggggttcg tcgagcagca gcagccgggg gcgggcggcg
     7441 agcgcgcggg ccagcgccac ccgcccggcc tggccgccgg agagctggcc ggggcggcgt
     7501 ccggcgagat ggcccacctc gagccggtcg agccagtgct gggccgcggc ccgcgcctcg
     7561 gcgcgcggga cgccctgggc gcgcggcccg tacgcggtgt tggcgagggc ggtgagatgc
     7621 gggaacaggg ccggtgactg ggggacccag gcggtggagc gccggtgcgg cggcgcgtcg
     7681 gtgaggtcgg tggcgccgag ccggagcgcg gcgtgggaac gggtgggcag cccgagcagg
     7741 gcgcgcagca gggtcgtctt cccggcgccg ttgggaccga cgaccgcgag ggtggtgccg
     7801 ccctcggcgg tgagggcgag ctcggtgaat ccggtgagcc gggcgtcgag ggcggtggcc
     7861 cggggcgcgc ggtcggcggg tacggagtcg ggtacgggtg cggggtcctg cgccgggtcc
     7921 ggtgcggagg ggccgggttc gggtgccgtt cgtggttcgg gggccgggtc acggtcggcg
     7981 cggggtgcgg cggtggccgg ggtcgtcgtc cagcggccgc ggagggcgat caggacggcc
     8041 atggcgatgg cgagcagcag cagggagacc gaggtcgccg cctccgggtc gtcctggagc
     8101 agcaggtaca catgcagggg gagggtctgg gtggtgccgg ggaggttgcc cgcgaaggtg
     8161 atcgtcgcgc cgaactcacc cagcgcgcgg gcccaggtca gggcggcgcc cgcggcgagt
     8221 ccgggggcga ccatcggcag cgtcaccgta cggaacaccc ggaacgggct cgcgccgagg
     8281 gacgccgcgg cctcctcgta gcgggtgctg agcgcggcga gcgtgccttc caggctgatg
     8341 accaggaacg gcatcgagac gaaggccgcc gcgacgacgg cgcccgcggt gtggaagggg
     8401 agggtgatcc cgaaccagtc ctccagccag gggccgagca gtccgcgccg tccgaagccg
     8461 aggagcagcg cgaccccgcc gacggtcggc ggcaggacca tcggcagcag gaccagggtc
     8521 cgcacgaacg ccttgccgcg gaacgggacg cgggccagca gccaggccag cggcaccccg
     8581 agcagcaggg cgagggcgag ggcccagccg gagacgacca gcgacagacg cagcgcggtg
     8641 accgccccgg gcgcggtcag atgctccgcg aggtcgcccc acggggtgcg ggcgaggatg
     8701 ccgagcaggg gcagcatcag gaacgcgacg gcgagcgccg cgggcagtgc gagggccagc
     8761 ggggggcggg ccggcggggt ggtgccgggt gcgcgggccg ggcgggtctt catggcgtga
     8821 tcctcgggga cggcgcggcg ggggcggcgg tgcccccgcc ggtgcggcgg gtcacggctt
     8881 ctggaagccc gcgtcctgga ggatcttctg ggcggcgggc gtggacagcc actccacgaa
     8941 cgtctgggcc gccgccacgt tcttcccgcc cttgatgacg gccgccgggt actgggcgat
     9001 ggcgttctgg tcgtcgggga cggtgatcgc gtcgaccttg tccgtcgcgg tttcggcgtc
     9061 ggtcttgtag acgattccgg cgtccgcctc gccgagttcg accttgctga ggacggcgcg
     9121 gacgttgggc tcctcggaca ccggctcgac atcgatcttc tgcgcgtcga gcacctggcg
     9181 gctgtagcgg ccgacgggga cctcgggtgc ggcgagcacg accttcagct tcgggtcggc
     9241 gaggtccttc agcccgtcga ccttgtcggg gttgccctcg cgggtggcga tgacgagccg
     9301 gttcctggcg atgaccgacg gggtgcccgt gtcggccttc agcccgtcca tggtcctggt
     9361 gtcggcggtg acgatcgcgt cggcgggggc gccctggcgg acctgggcgg ccagttcctg
     9421 ggagcccgcg aaggagaacg tgagggtggt gcccgggtgg gcgtcctcgt acgcgctctt
     9481 caccgtgccg aagacatcgg tgagggacgc ggcggcgagg accgtgagct tcgcggagtc
     9541 ctgggcgccg gagtccttct tctcctcgtc gtcgccgcag gcggcgagca gcggtatgag
     9601 gagcgcggtg gcggcgaggg cggtggcggc tgtgcgggcc ggggagcggg acatcgggga
     9661 ctccttggag gacttcgagg gtgcgggacg gacgggggag ccccgtcagc tccggtcgat
     9721 gtgcacattg gtggacttga cccgggcggt ggcctgcaca cccacctcca ggccgagttc
     9781 ctccacggcc tcccgggtga gcagggagac cagccggtgc ggtccggcct ggatctccac
     9841 ctgcgcggcc acgtcgccga gcttcacggc ggtgacgatg ccggggaacg cgttgcgggc
     9901 ggaggtgtag gagacgtcgt tctcgccctc gccctgggcg agttcgacgg agaacgcggc
     9961 gaggtccact ccgtcgacga gtcggcgccc gccctcgtcg cggtgcgtgg cgatccgccc
    10021 ggcgtcggcc cagcggcgcg cggtgtccgc gctgacgccg aggagacggg cggcctgtcc
    10081 aatggtgtac gacggcatgt gcgccacgat atggatgagg gggccgcatg tgcaagctaa
    10141 tggaggaatg tgcgtcgcat ttgcgggcct cttcgctctg tgccgcaggt tccgcggggc
    10201 gggggcgccg ctacgctacg tcctcatgac ccgacccgtg ccgtactcgc gcccgacgcc
    10261 cgatcggccc gctccggtgt gggtcatcag cgcggcatcg ctcgcggtct tcctggtcac
    10321 gtggacgctg agctgggtca aggcgtatct ggtgaacgac gatctgccca gcttctgctc
    10381 cgacgtccgg cgggggagtt ttccgcccga ggtgtcgtgc gtggccgggg acgggtcg
//
