LOCUS       NZ_LFBV01000001        10288 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces uncialis strain DCA2648 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_LFBV01000001
VERSION     NZ_LFBV01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces uncialis
  ORGANISM  Streptomyces uncialis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2018837)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Cloning and characterization of the uncialamcin biosynthetic gene
            cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2018837)
  AUTHORS   Yan,X., Huang,T., Ge,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33410, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-MAY-2015
            Assembly Method                   :: CLC Genomics Workbench v. 8.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905345.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,456
            CDSs (total)                      :: 7,371
            Genes (coding)                    :: 7,214
            CDSs (with protein)               :: 7,214
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 42 of 157
            Pseudo Genes (incomplete)         :: 143 of 157
            Pseudo Genes (internal stop)      :: 7 of 157
            Pseudo Genes (multiple problems)  :: 33 of 157
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 393472
            Orig. end                         :: 403760
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LFBV01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5288
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            556..2487
                     /locus_tag="AB852_RS01690"
                     /old_locus_tag="AB852_01675"
     CDS             556..2487
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577855.1"
                     /locus_tag="AB852_RS01690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_01675"
                     /product="VWA domain-containing protein"
                     /protein_id="WP_342748444.1"
                     /transl_table=11
                     /translation="MPAQGAEPDAASAPAQAAEQESAPVPAQATGPDPVPAAASTGTSG
                     SGGSEGATEAAGSERSEGDASGGTAEAGDTATAPAAGTGDAPAVKVPAQSSVADELVSA
                     AFDNVTVPRPAEPVRVPPTGVDLPAQSTGTDSPASATSPGTGADTPAAVPDAPAPAPAP
                     AVDTPDTPDTPDSPAPVAEKSPEPVAEVTPEPVAVAEPEPVAVAEPEVVAAAEPVAAEP
                     EAVTEPEPVVVAGPVAVEAGVTEPEPEPVVVIEPVTVGDEPVAVVAEPQPDPGTVAVSP
                     ETVAVPVPEPEAVTADPEPVAVVAEAEAVTVPEPVAVQVEAEPEPEPVAVEPEPVAVAV
                     EPDPEPVAVAAEPAAEESAAVPAEPEPEAIPAAVVPAPVTTDARTEADAATDTEAEADT
                     GSETDTDADTDTDAKPAYSLARVRSRAPGLVAGYKAAGAALRKHGLAGARLRVYLVLDR
                     SGSMRGYYKDGSAQSLGEQVLALAAHVDEEATVPLVFFSTEIDATGELGLDSYEGKVDE
                     LHTAAGRMGRTSYHAAVEAVVAHHEKSGATEPALVVFQTDGPPDVKGTATQALAEAARL
                     PLFFQFVAFGEHEAKGFDYLRKLKAGNAAFFHAGPVPRELTDKDLYEGLLAAFPGWAAA
                     RDGAADQS"
     gene            2663..4375
                     /gene="metG"
                     /locus_tag="AB852_RS01695"
                     /old_locus_tag="AB852_01680"
     CDS             2663..4375
                     /EC_number="6.1.1.10"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="metG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007383415.1"
                     /locus_tag="AB852_RS01695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_01680"
                     /product="methionine--tRNA ligase"
                     /protein_id="WP_073782852.1"
                     /transl_table=11
                     /translation="MARHLITSALPYINGIKHLGNMVGSMLPADVYARYLRQRGHDVLY
                     ICATDEHGTPAELAAKERGLPVAEFCAQAHDAQKAVYDGFELAFDYFGRSSSAQNAEIT
                     QHFARKLNENGFIEERAIRQVYSPADGRFLPDRYVEGTCPHCGYDKARGDQCENCTRVL
                     DPTDLINPRSAISGSTDLEVRETKHLFLLQSKLQPEVEAWIDQVGGEWPQLASSIARKW
                     LTEGLHDRAITRDLDWGVPVPADTWPELAADGKVFYVWFDAPVEYIAATKEWADAAPAG
                     EVRDWKSWWYEADDTVRYTEFMAKDNVPFHTVMFPATELGVREPWKKVDYVKAFNWLTY
                     YGGKFSTSQKRGVFTDAALDVLPADYWRYFLIANAPESDDSSFTWEHFAATVNKDLADT
                     LGNFVNRVLSFSKKRFGDEVPAGGEPGEAETRLGAETARLLAEYEEQMEALQFRKAAAA
                     LRALWSAGNSYLEEKSPWLEIKTDKDAAALTLRTAMNLIHLYSVVSEPFIPATARAMRG
                     AFTLVDDTAAWVAPQEAAALTAVSAGVPFTVPPVLFAKITDEDLEGFRVRFGS"
     gene            5001..5288
                     /locus_tag="AB852_RS01700"
                     /old_locus_tag="AB852_01685"
     CDS             5001..5288
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="AB852_RS01700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_01685"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_073782854.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.9e-14,
                     bitscore: 49.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKFARLGVVAVAAIIAGATPALAAVTYPGGGEWHHGKTGSLVY
                     SDYYHSTACHGATSIGAWTERSPDTSAGNWAWTDVPRAFTSVDEAYWRKC"
     gene            5409..7583
                     /locus_tag="AB852_RS01705"
                     /old_locus_tag="AB852_01690"
     CDS             5409..7583
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AB852_RS01705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AB852_01690"
                     /product="hypothetical protein"
                     /protein_id="WP_143201162.1"
                     /transl_table=11
                     /translation="MVHRGIKFVHAAVLAVATVLAFLVVSELDERTVLGHRAMLSVDRA
                     DGSTSGAGVVRALTEFADRHDTVIARSLPDLEDSEGRRHLYLAVGDRDSAAASWQRDGY
                     PDFGQRVDTEVHPMTALGNRDPRGVYYLIGPGSEAAAVAEFGALGLSVTVAHPLAPSEL
                     WSVYTDGPLTDVFLTTALALVTLTGAGVLLSARSYGVLRLHGLSLTGAFLRDLRQLRRS
                     WLISAGLLTVLVAVALGFLNGWARTGLYAAVAVGIAAVLGLTVLAAHAAALALVFRTAL
                     PGSLKGEVPVRAVTLTAYAVRIPALLLVVGLVGSVVTAGGDLLERREVWKAYARTGDAT
                     GVRITGSVSFDDLPGMERAMGRRLHQADREGKVVMAGRLPLGNLAGADVPGELLVVNEA
                     FLAEQPLHAPDGHRHQPDTHKDATDGKSGESGGGKVRLLVPEALGGSLPRLLERTPSIL
                     RPADPGRVPRSDIETRLLRDGQRVFTYTPGDRARDGSGEDRSFVRDPVVLVVPAASGHL
                     TEVNYYAFATRNQLFFPDPADIHDILREDESMRTYIAALLPVKQKAAVATAEAARTFRL
                     QLFELLAGTAVLLITAVGVCAVHTRSHARTVLVRHLVGWPFPATHRTLLAVEAAVLLLL
                     AGWLPARIAWENQELSGYTARGIPPPRPLAEITGTDVVTTAGLAVTSLGVLFCALVLFH
                     RRIVREGIGASRVPVLPRPLRKPSGARSRT"
     gene            7580..8218
                     /locus_tag="AB852_RS01710"
                     /old_locus_tag="AB852_01695"
     CDS             7580..8218
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374867.1"
                     /locus_tag="AB852_RS01710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_01695"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_073782858.1"
                     /transl_table=11
                     /translation="MIGIEKLSKSYGARTLWSGVDLTVHGGRMLALTGPSGSGKSTLLN
                     CLGLLDTATSGTVRHEGQDITRFGPRRARLFRRDVLGYLFQDYALIETATVAANLEVVA
                     GPGRRARAAARAAVPEALERVGLAGRAGERVARLSGGEQQRVALARLMVKRPSLVLADE
                     PTGALDQENTTTVVKILRELADEGRAVVVATHDDHVRDACDTVFRLGPR"
     gene            complement(8257..8844)
                     /locus_tag="AB852_RS01715"
                     /old_locus_tag="AB852_01700"
     CDS             complement(8257..8844)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958931.1"
                     /locus_tag="AB852_RS01715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AB852_01700"
                     /product="Uma2 family endonuclease"
                     /protein_id="WP_073782860.1"
                     /transl_table=11
                     /translation="MGVTEERPSVLSQEQFEELAHLGNRVDESLRLEFINGRIGEKAVP
                     DGVHGRIIVWLTQFCVQALPGMWLFGEQGIRVETYRKGNARPDGLLAPIDHFVEQGEWA
                     SPEGVLMVVEVTSTDSDTTSRDRVDKPRAYAESGIPVYLLIDLGTHEVKVHSLPDGVRY
                     ESMLTVPFGKTVPLPDPVGFDLDTEPLKSWVH"
     misc_feature    complement(8950..8963)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.56"
                     /tool="antismash"
     misc_feature    complement(9174..9189)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.17"
                     /tool="antismash"
ORIGIN
        1 ggcggcgcct tgcgggacgg tggccgcccg cgcctcggac gcgcccgtgc cgaggaccgt
       61 cccgcccgcg gcggcggcga ccgtcaccac ggccgccgtg cgcatcaccg cgcgacggct
      121 cagggctccg gcgatcacat cgcccgcgta ctcgttgccg ctggtgttcg ggacctcgcc
      181 gaagcaggcg tcgccgcagc ggtatctaca ggtcagggcg gagcgaccgc ccgcgtgcgt
      241 ggcgatcatc ggcagaagtt cgcgcacttc tcgctcccct tggtgtgcgt ccggtgtatg
      301 tgctttcgcc gtgaaactag gcgggcccga tcgcgggaaa agggacgaaa cgtgaacgcg
      361 gggtgaatcc ggcgcgcagg gcaggggctg ccgccgggcc ccgggggtgg gtcggccggt
      421 gatcatccgg gcgtttggct aaccttacgt ggccgtcctg gccagggatc ggcggaacaa
      481 ctcatctgaa gggttgcgca catgggcatt cttagtcgac tgcgggatgt gttcgggcgg
      541 tcgcggaagg ccgaggtgcc tgcgcagggt gccgagccgg acgccgcatc cgcgccggca
      601 caggccgctg agcaggagtc cgcgcccgtc cccgcgcagg ccacggggcc cgatccggtg
      661 cccgcggcgg cgtccaccgg gacctccggg tccggcggga gcgagggcgc cacggaggcg
      721 gccggatccg agcggtccga gggggacgcg tccggcggga ccgccgaggc cggggacacg
      781 gctaccgctc cggcggccgg taccggcgac gccccggcgg tgaaggtccc cgcgcagtcg
      841 tccgtcgccg acgagttggt ctccgccgcg ttcgacaacg tcacggtgcc gcgtccggcg
      901 gagccggtac gggtgccgcc gacgggggtg gacctgccgg cgcagtcgac gggtacggat
      961 tccccggcgt cggccacatc gccgggtacg ggcgccgaca ccccggccgc cgttcccgat
     1021 gccccggccc cggccccggc cccggccgtg gacaccccgg acaccccgga cacccccgac
     1081 tccccggccc cggttgccga gaagtccccg gagccggtgg ccgaggtgac ccccgaaccg
     1141 gtggccgtgg ctgagccgga gccggtggct gtggctgagc cggaggttgt cgccgctgcc
     1201 gagccggtgg ccgccgagcc ggaagccgtg acggagcccg agccggtcgt ggtcgccggg
     1261 ccggtcgccg tcgaggccgg ggtgacggag ccggagcccg agccggtcgt ggtcatcgag
     1321 ccggtcaccg tcggggatga gccggtcgcc gtggtcgcgg agccgcagcc cgaccccggg
     1381 acggtcgcgg tgtcccctga gacggtcgcc gtgccggtac cggagccgga agccgttacg
     1441 gcggaccccg agccggtggc cgtcgtggcc gaagcggagg ccgtcacggt tcccgagccg
     1501 gtcgccgtac aggtcgaagc cgagcccgag cccgagccgg tcgccgttga gccggagccg
     1561 gtcgccgtag cggtggagcc cgaccccgag cccgtcgccg tcgcggccga acccgccgcc
     1621 gaagagtcgg cggccgtacc cgccgagccc gagcccgagg cgatcccggc ggccgtggtc
     1681 cccgcccccg tcaccacgga cgcgcgcacc gaagcggacg cggcaacgga caccgaagcc
     1741 gaagcggaca caggttccga gacggacacg gacgcggaca cggacacgga cgcgaagccc
     1801 gcgtacagcc tcgcccgggt ccggtcccgc gctcccgggc tggtcgccgg gtacaaggcc
     1861 gcgggtgccg cgctccgcaa gcacgggctg gccggtgcca ggctgcgggt ctacctcgtg
     1921 ctggaccggt ccgggtcgat gcgcgggtac tacaaggacg gctccgcgca gagcctgggc
     1981 gagcaggtgc tcgcgctcgc cgcgcatgtg gacgaggagg cgaccgtccc cctggtgttc
     2041 ttctccaccg agatcgacgc gaccggtgaa ctcggcctcg actcgtacga gggaaaggtc
     2101 gacgagctgc acacggcggc cgggcggatg ggccgcacca gctaccacgc ggccgtcgag
     2161 gcggtcgtcg cgcaccacga gaagtcgggt gccacggagc ccgcgctggt cgtgttccag
     2221 accgacggtc cgccggacgt caagggcacg gccacccagg ccctcgccga ggcggcgcgg
     2281 ctgccgctgt tcttccagtt cgtcgcgttc ggtgagcacg aggcgaaggg cttcgactac
     2341 ctccgcaagc tgaaggcggg caacgccgcg ttcttccacg cgggcccggt cccgcgtgag
     2401 ctgaccgaca aggatctgta cgaaggtctc ctcgccgcgt tccccgggtg ggcggcggcg
     2461 cgcgacggag ccgccgacca gagctgaccc agggccggcc atcggctgac caccgcgttc
     2521 gcacaggtcc cacgggggcg tcccgccgga aattgccggg cggggcgccc ccgcctgtcg
     2581 gatacgattt gtggattccg cgcccggcag caccacacag gcgcgatccg caccacccgc
     2641 agcgacctgg gagcagcccg cgatggctcg acacctcatc accagcgccc ttccgtacat
     2701 caacgggatc aagcacctgg gcaacatggt ggggtccatg ctcccggctg atgtctacgc
     2761 gcggtacctg cgccagcgcg gccatgacgt cctgtacatc tgcgcgaccg acgagcacgg
     2821 caccccggcc gagctggctg ccaaggagcg cgggctgccg gtggccgagt tctgcgcgca
     2881 ggcgcacgac gcgcagaagg cggtgtacga cggcttcgag ctggccttcg actacttcgg
     2941 ccgcagctcc tcggcgcaga acgccgagat cacccagcac ttcgcccgca agctgaacga
     3001 gaacgggttc atcgaggagc gggcgatccg ccaggtgtac tcgccggccg acgggcgttt
     3061 cctgccggac cggtacgtcg agggcacgtg tccgcactgc gggtacgaca aggcgcgcgg
     3121 cgaccagtgc gagaactgca cccgggtcct ggaccccacg gacctgatca acccgcgttc
     3181 ggcgatcagc ggctccacgg acctggaggt ccgcgagacc aagcatctgt tcctgctcca
     3241 gtcgaagctc cagcccgagg tcgaggcgtg gatcgaccag gtcggcggcg agtggccgca
     3301 gctcgcgtcg tccatcgccc gcaagtggct gaccgagggc ctgcacgacc gtgcgatcac
     3361 ccgtgacctg gactggggcg tgcccgtccc ggccgacacc tggccggagc tggcggccga
     3421 cggcaaggtc ttctacgtct ggttcgacgc gccggtcgag tacatcgccg cgacgaagga
     3481 gtgggccgac gccgcgcccg ccggtgaggt ccgcgactgg aagtcgtggt ggtacgaggc
     3541 cgacgacacg gtccggtaca cagagttcat ggccaaggac aacgtcccct tccacaccgt
     3601 gatgttcccg gcgacggagc tgggtgtgcg ggagccgtgg aagaaggtcg actacgtcaa
     3661 ggcgttcaac tggctgacgt actacggcgg caagttctcc acgtcgcaga agcggggcgt
     3721 cttcacggac gcggcgctcg atgtgctgcc cgccgactac tggcgctact tcctgatcgc
     3781 caacgccccc gagtccgacg actcgtcgtt cacctgggag cacttcgccg ccacggtcaa
     3841 caaggacctc gcggacaccc tcggcaactt cgtcaaccgg gtgctgtcgt tctcgaagaa
     3901 gcggttcggg gacgaggtcc cggcgggcgg cgagcccggc gaggccgaga cgcggctcgg
     3961 cgcggagacc gcgcggctcc tcgcggagta cgaggagcag atggaggccc tccagttccg
     4021 caaggccgcg gcggcgctgc gcgcgctgtg gtcggcgggc aactcctacc tggaggagaa
     4081 gtccccctgg ctggagatca agacggacaa ggacgcggcg gcgctgacgc tgcggaccgc
     4141 gatgaacctg atccatctgt actcggtggt gtccgagccg ttcatccccg cgacggcgcg
     4201 ggcgatgcgg ggcgcgttca cgctggtgga cgacacggcc gcgtgggtcg cgccgcagga
     4261 ggcggcagcg ctgaccgccg tgtccgccgg ggtgccgttc acggtgccgc cggtgctctt
     4321 cgcgaagatc acggacgagg acctggaagg gttccgtgtg cggttcggat cgtagccaca
     4381 ggagcgtggg agggggcccg gacctggcgg tccgggcccc ttcccgtgtg tctgagggga
     4441 ccgacccggc ccgcgcccgg ggattcccgc acctggccgt gtcccttccc cgcgtggtca
     4501 cccggagtca ggggctgggt ggcgtgatcc cgccttgcac gggcgggtgc gggtggcgtg
     4561 cttgtggggg gtgtgagtcg gagggcgggg ggtgtccgac ccccgccaag cagggggttc
     4621 cgttcgggag ccgggattcc cgggccggtt cgagcggctc tcgatcgccc cctcttcgca
     4681 ttcgtgaagg gtcgagccag tggtgaacgc gccaacttga gtgcgggtat ccggtgacgg
     4741 cgtgtggtgc gtaccgtgcg gtctcgtctc tcatgcgaac ttcccgctgc cgtgttcaga
     4801 gggccggacc ggcgcggcgc gcgccccgat ccgtaccagc cccgccgtca ctccccgcgt
     4861 acgaacgggt gcggagaggg ggtgaaacca tccgaaggtg ggcgtcccgg aggctgaccc
     4921 gttagcgggt gtcgggcggt cgtcggccgt tcctatggtg agcgggctcc gaagacatcg
     4981 gggttctggg agggatcacc atgaccaaga agttcgccag gctcggcgtg gtcgcggtcg
     5041 ccgcgatcat cgccggcgcg acacccgcgc tcgccgccgt cacctacccg gggggcggtg
     5101 agtggcacca cgggaagacc ggctccctcg tctactcgga ctactaccac tccaccgcgt
     5161 gccacggcgc gacctccatc ggcgcctgga cggagcgcag ccccgacacc tcggccggga
     5221 actgggcgtg gaccgacgtc cccagggcgt tcacctctgt cgacgaggcg tactggcgca
     5281 agtgctgacc cgctggccgg tgcgggtccg gccgcggcac tgaatccttc cggccggtgg
     5341 ggagcggcag ccctccaccg gcctctgtgc cccgattcgc cacttcgacg agggaacaga
     5401 cgggtcagat ggttcaccga ggaatcaagt tcgtgcatgc cgccgtgctg gccgtcgcga
     5461 cggtgctggc ctttctcgtc gtctccgaac tcgacgagcg gacggtgctg ggccaccggg
     5521 ccatgctgtc cgtcgaccgg gcggacggct ccacgagcgg ggcgggcgtg gtccgggcgc
     5581 tgacggagtt cgcggaccgg cacgacacgg tgatcgcgcg gtcgctgccc gacctggagg
     5641 actcggaagg gcgccgccat ctgtacctcg cggtggggga ccgggactcg gccgccgcgt
     5701 cctggcagcg ggacggctac ccggacttcg ggcagcgggt cgacaccgag gtccacccga
     5761 tgaccgccct cggcaaccgt gatccccggg gtgtctacta cctcatcgga cccggttccg
     5821 aggcggcggc agtcgcggag ttcggcgccc tcggactgtc ggtgaccgtc gcccaccccc
     5881 tggcaccgtc cgagctgtgg tccgtgtaca cggacggccc gctcaccgat gtcttcctga
     5941 ccacggccct cgccctggtc acgctcaccg gcgcgggcgt gctgctgagc gcgcggagct
     6001 acggagtcct ccggctccac gggctgtccc tgaccggggc gttcctccgt gacctgcggc
     6061 aactgcgccg atcctggctg atctccgcgg gcctgctgac cgtactggtg gcggtggcgc
     6121 tcgggttcct caacggctgg gcccggacgg gtctctacgc ggcggtcgcc gtgggcatcg
     6181 cggcggtact gggcctgacc gtgctcgcgg cccacgccgc cgcgctcgcg ctggtcttcc
     6241 ggaccgctct gccggggtcg ctcaaggggg aggtgcccgt ccgggccgtc accctgaccg
     6301 cctacgcggt gcgtattccc gcgctgctgc tcgtcgtcgg gctcgtcggg tcggtcgtca
     6361 ccgcgggagg ggatctcctg gagcgccgcg aggtgtggaa ggcgtacgcg cggacggggg
     6421 acgcgaccgg tgtccggatc accgggagcg tgtccttcga cgatctgccc gggatggaac
     6481 gggcgatggg ccgcaggctt caccaggccg accgggaggg gaaggtcgtc atggccggac
     6541 ggctcccgct ggggaatctg gccggcgcgg atgtgccggg tgaactgctc gtcgtgaacg
     6601 aggcgttcct ggccgaacag cccctccacg ccccggacgg ccaccgccac cagccggaca
     6661 cccacaagga cgcgacggac gggaagtccg gcgagtcggg cggcggcaag gtcaggctgc
     6721 tcgtccccga ggcactgggc gggtcgctgc cccggctgct ggagcggacg ccctcgatcc
     6781 tgcggcccgc cgaccccgga cgcgtccccc ggagcgacat cgagacccgt ctcctgcgcg
     6841 acgggcagcg ggtcttcacc tacacccccg gggaccgggc ccgggacggg agcggcgagg
     6901 accgctcctt cgtacgcgat ccggtcgtcc tcgtcgtccc ggccgcgtcc ggccatctca
     6961 ccgaggtcaa ctactacgcc ttcgccaccc gcaaccagct gttctttccc gacccggcgg
     7021 acatccacga catcttgcgg gaggacgagt cgatgcgtac gtacatcgcg gcgctcctcc
     7081 cggtgaagca gaaggcggcc gtcgcgacgg ctgaggcggc ccggacgttc cggctccagc
     7141 tgttcgaact gctggcgggg acggccgtgc tgctcatcac cgccgtgggg gtgtgcgcgg
     7201 tgcacaccag gtcccacgcg cggacggtcc tcgtccggca cctcgtcgga tggccgttcc
     7261 cggcgaccca ccggaccctg ctggcggtgg aggcggccgt cctgctgctt ctcgccgggt
     7321 ggctcccggc gcggatcgcc tgggagaacc aggagctcag cggatacacg gccaggggca
     7381 tcccgccccc gcgtccgctc gcggagatca ccgggacgga cgtggtgacg acggccggcc
     7441 tcgccgtcac ctcgctcggt gtcctgttct gcgcgctcgt cctcttccac cggcgcatcg
     7501 tccgggaggg catcggggcg tcccgtgtcc cggtcctccc ccgccccctc cgcaagccgt
     7561 caggagccag gagccgcacg tgatcggtat cgagaagctg tccaagtcct atggcgcgcg
     7621 caccctgtgg tcgggcgtcg acctcaccgt ccacggcgga cggatgctcg ccctcaccgg
     7681 cccgagcggc tccggcaagt cgacattgct caactgtctc ggactgctcg acacggccac
     7741 gtccgggacg gtccggcacg agggccagga catcacccgc ttcgggccgc gccgggcccg
     7801 gctcttccgc cgcgatgtcc tcggctatct cttccaggac tacgccctga tcgagaccgc
     7861 gaccgtcgcg gccaatctgg aggtcgtcgc cgggccgggc cggcgggcgc gggcggcggc
     7921 gcgggcggcc gtccccgagg cactggagcg ggtggggctg gccgggcgcg ccggggagcg
     7981 ggtcgccagg ctcagcgggg gcgagcagca gcgtgtggcg ctggcgcggc tcatggtcaa
     8041 acggccgtca ctggtactcg ccgacgaacc caccggagcc ctcgaccagg agaacaccac
     8101 gacggtcgtg aagatcctcc gcgaactggc cgacgagggc cgcgcggtcg tcgtcgccac
     8161 ccacgacgac cacgtacgcg acgcgtgcga cacggtgttc cgcctgggcc cccgctgaac
     8221 gggacccggc tccggggccg ggtggcggac gtgcggtcag tggacccagg acttcagcgg
     8281 ctcggtgtcc agatcgaagc cgaccgggtc ggggaggggg accgtcttgc cgaagggaac
     8341 ggtgagcatt gactcgtagc gcacgccgtc cggcagggag tggaccttca cctcgtgtgt
     8401 ccccaggtcg atcagcagat agaccgggat accgctctcg gcgtacgcgc gcggcttgtc
     8461 gacgcgatcc cgactcgtcg tgtccgagtc ggtggaggtg acctccacga ccatgaggac
     8521 gccctccggg ctcgcccact cgccttgttc gacgaagtgg tcgatgggcg cgagcagccc
     8581 gtcggggcgc gcgttgccct tccggtaggt ctcgacgcgg atcccctgct ccccgaagag
     8641 ccacatccca ggcagagcct gcacacagaa ctgggtcagc cagacgatga tccgcccgtg
     8701 tacaccatcc ggcactgcct tctccccgat ccttccgttg atgaactcca agcgcaggga
     8761 ttcgtccacg cggttgccca ggtgcgcgag ttcctcgaac tgctcctggc tcaggacgct
     8821 cggacgctcc tcggtgacac ccacgatggt ctcctcctgg atcggtggtg attcccaggg
     8881 taagggcccg ggagtcgccc gggccggatt cgttgcgagc ttcgccatgg gcgggagcct
     8941 agggaagcgg cacggagtgt gacgtaccgt ccagggaatc gtcacaccat gtgatgaagt
     9001 gcgggtgggg atcttgtcgc gggggacggg gtgtgaacct ctgggggccg gtgggagtgg
     9061 ggaggtcggg ggacgccgga gggcccgtga ccgaccccgg ggaactccgg gtggatcacg
     9121 ggccctccgg tcgtcggcgg ttcctcgccc cgctgacttc tacttctggc gatgtcaaaa
     9181 aaacctgaca tcgccagaag tagaagtcag cgcacgggcc cgccgggggc ggactacttc
     9241 ggctggggct tgcggatcgt gacgtgcagc tcgcgcagcc gcgtctcgtc cagctcggtc
     9301 ggcgcgccca tcatcaggtc ctgggcgttg ccgttcaggg ggaaggcgat ggtctcgcgg
     9361 atgttgggct cgtcggcgag cagcatgacg atccggtcga cgcccggggc gatcccgccg
     9421 tgcggcgggg cgccgaagcg gaacgcgcgg agcatgcccg cgaactcctg ctccacggtc
     9481 tcgttgtcgt agcccgcgat ctcgaacgcc ttcagcatga cctcgggctc gtggttgcgg
     9541 atcgcgccgg aggacagctc cacgccgttg cacacgatgt cgtactgcca ggccaggatg
     9601 tcgagggggt ccttctcctc caggtccttc atcccgccct gcggcatgga gaacgggttg
     9661 tgggagaagt cgatccgccc ggtctcctcg tccttctcgt acatcgggaa gtccacgatc
     9721 cagcagaacc ggaacacgcc ctcctcgaag tgcccggcgc gcttcgcggc ctcgacgcgg
     9781 accgcgccca tgatcttcga gacctcgtcg tactcgcccg cgccgaagaa cacggcgtgc
     9841 ccggcggcca gcgacaggcg cttggtcagc tcggcgacgt cgtcctcggt gaggaacttc
     9901 gcgatcggac cggtcagcga accgtcctcg gcgacccgga cccaggccag gcccttcgcg
     9961 ccctgctcga cggcgtactc accgagctgg tcgaagaact tgcggggctg ccccgacacg
    10021 tccggcaccg gcagcgcgcg tacatgcttg cccgcgaacg ccttgaagcc gctgtccgcg
    10081 aagacgtccg tgaggtcgac cagttcgagc ttggcccgca ggtcgggctt gtcggagccg
    10141 tacttcgcca tcgactcgcg gaacgggatg cgcgggaacg gcgaggtgac gggacggccg
    10201 ccgccgaact cctcgaacag ctccgtcatc agcttctcga tcggacggaa cacgtcctcc
    10261 tgctcgacga aggacatctc cacgtcga
//
