LOCUS       NZ_LWLE01000037        11356 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold8, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000037
VERSION     NZ_LWLE01000037.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 443716)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443716)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 176516
            Orig. end                         :: 187872
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11356
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11356
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11356
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6356](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6356)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(93..785)
                     /locus_tag="A6A29_RS39745"
                     /old_locus_tag="A6A29_22100"
     CDS             complement(93..785)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157694.1"
                     /locus_tag="A6A29_RS39745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22100"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_073728430.1"
                     /transl_table=11
                     /translation="MPSRTPVPRSRRERPAKPALTYEGIVATAVHLMETEGLRRVTMRR
                     LAQELDTGPASLYVYVANTAELHAAILEELLGAVDLSPVDGEGDWRGRLTLLLESYTHI
                     LFQNPGLAHSALVARPSGPHYLALVEAVLVLLHQGQVPDEQAAWGVDVLLQVATATAAE
                     QSSRARSADAADEHNALVVALRDVSSDTHPRIAALGDDLVSGAPGQRLAWVFRAVINGT
                     RATPRDTP"
     gene            1137..1661
                     /locus_tag="A6A29_RS39750"
                     /old_locus_tag="A6A29_22105"
     CDS             1137..1661
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182380.1"
                     /locus_tag="A6A29_RS39750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22105"
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="WP_079183232.1"
                     /transl_table=11
                     /translation="MHPDRVTATPPPAPGEPTHSPGTELRDTPGKGAGVFATHAFRIGE
                     VVLAGAIKAELDRNDAHASQIGPDRFIRHAGLMPRVNHSCDPNCGVQVNDSGAHDLVAR
                     FPVHVGQEVTFDYAMRNHTVQHFPPRCQCGSRLCRGRITGWQDLPSDRRHAYRGYVAPY
                     LLHLPQGPQPR"
     gene            complement(1779..2603)
                     /locus_tag="A6A29_RS39755"
                     /old_locus_tag="A6A29_22110"
     CDS             complement(1779..2603)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145612.1"
                     /locus_tag="A6A29_RS39755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22110"
                     /product="aldo/keto reductase"
                     /protein_id="WP_073728432.1"
                     /transl_table=11
                     /translation="MQCLTLNNGISMPRVGLGVWPLTDDQAYTAVTHALGCGYRRIDTA
                     RVYDNEEGVGRAIRDAAIPRQEVFLTTKLWNADHGYDAALHAFDASIRRLGTDYVDLYL
                     IHWPIPRRNAYVESYRALERIYAEGRAKAIGVSNFTAATLGRLLAETGIVPALNQIELH
                     PYFPQRAMRTFAAERSMVTEAWSPLAQGQQLLAEPVLARIAERKGKSPAQIVLRWHLQL
                     GNTVVPRSVTPSRIRENLSVYDFALGPQDLRDIATLDNGVRIGPDPDTVVQR"
     gene            complement(2726..3928)
                     /locus_tag="A6A29_RS39760"
                     /old_locus_tag="A6A29_22115"
     CDS             complement(2726..3928)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="A6A29_RS39760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22115"
                     /product="cytochrome P450"
                     /protein_id="WP_073728434.1"
                     /sec_met_domain="p450 (E-value: 4.3e-21, bitscore: 69.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHADPEPPPHTVDFPFPHHDGLGPLPEFAHLRREAPCARIRLPS
                     GDPAWLVTRYADVRTVLADPRFSRRRATQGTGPRIARVPTLPDSILAADPPEHSRLRKL
                     VAPAFTVRRAEAMRRDVALLVDELLSTLAAHDRPADLVALLARPLPLAVICDLLGIPRQ
                     DGDRLDTWCDRLRNLTASSDTEVTAAVGEMTGYLGALVAAKRRHPGNDLLSTLISARDE
                     DDRLSQDELISFSLVLLAGGYGTTADRLAGMAHLLLDEPQRYERLRREPGTIPKAVEEL
                     LRYAQTNVQANLRVATEEVELGGNTIAEGDAVMAVNSSANHDETVFENPSLLDFDRDRN
                     PHMGFGHGVHHCVGAQIARVQLQEALAGLVRRFPGLRAAAPPEWKTGLKTRAPRSLLVT
                     W"
     gene            complement(4072..4902)
                     /locus_tag="A6A29_RS39765"
                     /old_locus_tag="A6A29_22120"
     CDS             complement(4072..4902)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="A6A29_RS39765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22120"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_079183233.1"
                     /transl_table=11
                     /translation="MPTFHDHPAPAFTAATGSAPDNAGDPGNAPAQPPAPSPGYADIHA
                     DDYDRWFGKPGITAATVDTLASLAGDGPVLELGIGTGRVALPLAARGLDVHGVEASAAM
                     AARLRSKPGGDRVHVTLGDFADLPVADTYSLVHVVGGTFFELAGRDAQQRCLTAVATHL
                     EPTGVFVLDAHLPEALAVAAASGVPEVVSETDEHLILCHRRIDPSTQTYQSHYLIHEAD
                     RTRHLRVHFRYASPGELDLMAERAGLRLRQRLGSWRGGPLAPDSTYHVSVYELR"
     gene            complement(5001..6356)
                     /locus_tag="A6A29_RS39770"
                     /old_locus_tag="A6A29_22125"
     CDS             complement(5001..6356)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="A6A29_RS39770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22125"
                     /product="YcaO-like family protein"
                     /protein_id="WP_073728436.1"
                     /sec_met_domain="YcaO (E-value: 2.7e-34, bitscore: 113.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVSPWEPQVFTPYAAWPEVVFARVAARSPQFDPVCAAGNRPIVV
                     GSAAGHRPPRVVASARGELLERVSNILAGRAAESDAGIVATHGELRRKGIPALDPAELM
                     SGGPAGTVDDFRHARRLWVRGRSLRTGADMHVPAGAVFLHHRPPAGCDPGPGAGSTGLA
                     AHPDPEAAVQHAAWEVLERDLIRRSWYGLSRAPQVIPAGELPGPLAKLLDGIGVTTTVL
                     DLPAPVGSRCVAVCLHAPDGTRQAFGARCGPDGQGIAPLIEKAAYEALMVRWSVHTPVA
                     EDTWTRRQGRDAPDSAVEHALWAYHRQDSLRLWTERAPLAPRETPPPSAGPDRVGPGPR
                     TGPDRSGTESHTVADPSGTVSHTVADPSGNGSHTVADPSGNGSHTVADPSGTAVLSSHT
                     GQDLILVDTTCEPARGLGSAVVRVVAPGAHALPTGPDDERHLPAAPGPHPFG"
     gene            complement(6356..7270)
                     /locus_tag="A6A29_RS45640"
                     /old_locus_tag="A6A29_22130"
     CDS             complement(6356..7270)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="A6A29_RS45640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22130"
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_073728438.1"
                     /transl_table=11
                     /translation="MTRSALERAGLDVSPEEAVHPAVVEAAHIAQSVLRKGPPGPEGIA
                     RLSGMLARARSGIADTPPSGLPWPVGLAAPQPHLERSVARALKAVPRTDGDIGDRSRSA
                     VVAWREGDTEVIRGTIALLADIWPGAYAELKEILRQTVLLDGPALDGFTDFAVHGAVFI
                     RRDRLGPDTGGLPGTVRLAEALVHEGTHTRCNAASVAAEPFLRPAADGGPLVTTPLRLD
                     PRPLTGLFQQMVVLARSVLLYRRLLGETAADTRTTAAVRARHDELARSATDAVHIMTGH
                     AEALTDHGEAVLRDAAEVVRVRA"
     gene            complement(7399..7548)
                     /locus_tag="A6A29_RS45645"
     CDS             complement(7399..7548)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6A29_RS45645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158074508.1"
                     /transl_table=11
                     /translation="MPCVPWRRRDSPSDVGGAGRRREIDMHEEIEQVPEEELPELALMI
                     DVPS"
     gene            8510..9667
                     /locus_tag="A6A29_RS45650"
                     /old_locus_tag="A6A29_22135"
     CDS             8510..9667
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /locus_tag="A6A29_RS45650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22135"
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_073728440.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.2e-19,
                     bitscore: 63.8, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRISYLHGGSIPSLFANGVHVMRMCDAFAAAGHDITLYSMPGPDP
                     AEDPYTYYGVSNRFPIRLVAAPDHTPNGYWQRADTIRRMVARGPRPDIVYGRDPYALSV
                     LSDLAPFVYEVHQLRKDLVPVGTERKLLGKPGLARVVAITHALAGDLRRKYEDLGTLPI
                     LVAPDCADPPGPTATTQAPQLPGRPDVPRVGYVGHLYDGRGIGLVLDLADRFPGLDFHL
                     VGGTAEDRARWEKACRASHVYFHGHRPPSALPAYYGLFDIVLAPYETKVYTWGRLGETG
                     RWASPMKVFEYMSHGLPMIASDLPVLREVLQDGVNCLLRPPEDPAAWAEALGELVADGA
                     LRRRLGDEARRQVLERYTWRRRADDVLADIGHPDRPGTGEAGLPR"
     misc_feature    complement(8550..8565)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 24.49"
                     /tool="antismash"
     gene            9664..11124
                     /locus_tag="A6A29_RS39785"
                     /old_locus_tag="A6A29_22140"
     CDS             9664..11124
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967467.1"
                     /locus_tag="A6A29_RS39785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_22140"
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="WP_338012917.1"
                     /transl_table=11
                     /translation="MTEPGAAQSRSDAAALPGAAACGEAPLPTGASVDGYPARPSVRAG
                     EILRFHVSTRAERFRADFYRCGARPRLMGSTAGPGRYAPPGRHDEDWHWPGHDFAVPGD
                     WPSGVYIAVFGPGSARPAAAPPAGAEALDARHSRMLFVVTAREPAHRTRILYKVPVFTY
                     HAYNRSGGASLYGALPHDEAYRTITLRRPGGGIGGPVKGQPDAHDPRTPRQTFAHWDAP
                     FISWLETSGHTVDYCTDLDLHESPRHASGYRLLLSAGHDEYWSARTRRHITAFRDRGGN
                     IAVFGANTCWWRVTPTADGTAIRCAKYPPGVPQGSDCDRMRGAPDHWWEGEPENSLIGV
                     SYRNGGGHWAGLREPLGFTLQHTGHPVFAGTGLSDGDVLGEEHALVGYECDGAAHRRDP
                     LGRAVATGTDGTPADFTILGVADLPTAPDSGWYTAARENTDPGRAATFGLYTRGGTVFN
                     AATTDWARLLTLDPLVDAITRNVISLLR"
     misc_feature    complement(11277..11291)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.26"
                     /tool="antismash"
ORIGIN
        1 caccgatgac ggcgatgggg tagtggggtg tggtgctcat ggtggtgctc tccttcggtc
       61 cgggggcgga aagccgcagg cggacagggg tgtcacggtg tgtcgcgcgg ggtggcacgg
      121 gtgccgttga tcaccgcgcg gaagacccag gcgaggcgct gtcccggggc tccggagacg
      181 agatcgtcgc cgagcgcggc gatgcgcggg tgggtgtcgg aggagacgtc gcgcagcgcg
      241 acgaccaggg cgttgtgctc gtccgccgcg tcggcggatc gagcacggct cgactgctcg
      301 gcggcggtgg cggtcgccac ctggaggagc acgtccacgc cccaggcggc ctgctcatcg
      361 ggcacctggc cttggtggag cagcacgaga acggcttcca ccagggccag gtagtgaggg
      421 cccgaaggcc gggcgaccag ggcggaatgg gccaggccgg ggttctggaa caggatgtgc
      481 gtataggact ccagcaacag cgtcagccgc ccgcgccagt ccccctcgcc gtccaccggg
      541 gagagatcga cggcgccgag gagttcctcc aggatcgccg cgtgcagctc ggccgtgttg
      601 gccacgtaca cgtacagcga ggcgggaccg gtgtccagtt cctgggcgag ccggcgcatc
      661 gtgacgcgcc gcagtccctc ggtctccatc aggtggacgg cggtggcgac gatgccctcg
      721 taggtgagcg cgggcttggc gggccgctca cgacggctgc ggggcacggg ggttctcgaa
      781 ggcatgcgaa cagcgtaacg aacacgttcg ttacgaacaa gttcgtggcc gatttccgga
      841 tgcgtgcggc cggacgatcc cggctcattc ccgccgtagg gcttccaccc cgacgtgagg
      901 cgatcgcgcc cttcggtgca ggtcaatgtc ccgacaacgg cggacaccgg cgttcccgca
      961 ccgaagctcc ggccgtccac atggccggtg aactccgctg tccggccggt atccgcctgc
     1021 ccatcggggt agcgggccgc ttgtatggaa cacgacagcg ttccggcgcc gtccagagca
     1081 gttcgatcct cagcgggccg cacaagagcc cgccactacc gaccgaaggg ctccgcatgc
     1141 accccgaccg agtgaccgcc accccgcccc ccgcaccggg cgagccgacg cacagcccgg
     1201 ggacggagct gcgggacaca ccgggcaagg gagcgggggt cttcgccacc catgcgttcc
     1261 ggatcgggga ggtggtgctg gccggtgcga tcaaggccga gctcgaccgc aacgatgccc
     1321 acgcctcaca gatcggtccg gaccggttca tccggcacgc cggcctgatg ccccgcgtca
     1381 atcactcgtg cgatccgaac tgcggagtcc aggtcaacga ctccggcgct cacgacctcg
     1441 tggcccgttt cccggtgcac gtcgggcagg aggtcacctt cgactacgcc atgcggaacc
     1501 acaccgtcca gcacttcccg ccccgctgcc agtgcggatc ccggctgtgc cgcggccgga
     1561 tcaccggctg gcaggacctc ccgtcggatc gcaggcacgc ctaccgcggc tacgtcgctc
     1621 cctatctcct ccacctgccg caaggacctc agccccgctg acagaccgaa actgaacgcg
     1681 gacgagcgga ctgagctgac tggacagcgg ggcagcacac agcgggacta cgaaacagcg
     1741 ggacaggtga cgccgttggt gccggctgta cagccgactc accgctggac aacggtgtcc
     1801 gggtcgggcc cgatccggac accgttgtcc agggtggcga tgtcccgcag gtcctgcggg
     1861 ccgagagcga agtcgtagac gctcaggttc tccctgatcc gggagggggt cacggatctg
     1921 gggaccaccg tgttcccgag ttggagatgc cagcggagga cgatctgggc cggtgacttc
     1981 cccttcctct ccgcgatccg ggcgagaacg ggttcggcga gcaactgctg cccctgggcg
     2041 agcgggctcc acgcctccgt gaccatggac cgctcggcgg cgaacgtacg catggcgcgc
     2101 tgcgggaagt aggggtgaag ctcgatctgg ttcagggccg gcacgatgcc ggtctccgcc
     2161 aggagtctgc ccagggtcgc ggcggtgaag ttggagacgc cgatcgcctt cgcacgcccc
     2221 tcggcgtaga tccgttccag cgctctgtac gactcgacgt acgcgttacg ccgagggatg
     2281 ggccagtgga tcaggtagag gtcgacgtag tccgtgccca ggcgccggat gctcgcgtcg
     2341 aacgcgtgga gcgcggcgtc gtatccgtgg tcggcgttcc agagtttggt ggtgaggaac
     2401 acctcctgac gcgggatcgc cgcatctctg atcgcgcggc cgacaccctc ctcgttgtcg
     2461 tagacccggg cggtgtcgat acggcggtag ccgcacccaa gggcgtgagt gacggcggtg
     2521 tacgcctggt catccgtcag cggccagacg ccgaggccga ctcgcggcat ggagatgccg
     2581 ttgttgaggg tgaggcactg catacgggag ccttccgatc cgtggtacgg gaacgggcgt
     2641 gggcacggca cgagcacgac acgggcacgg gcacgggtga cgggtgacgg gcacgggtga
     2701 cggtggggcg ctcgcggtga acggctcacc aggtcacaag gagcgaacga ggtgcccggg
     2761 tcttcaagcc ggtcttccac tcgggcggag ccgccgcgcg caggccgggg aaccggcgta
     2821 cgagtccggc cagggcctcc tggagctgga cgcgggcgat ttgggcaccg acgcagtggt
     2881 ggacgccgtg tccgaagccc atgtgcggat tgcggtcacg gtcgaagtcg agcagagagg
     2941 ggttctcgaa gacggtctcg tcgtgattgg cggacgagtt gaccgccatg accgcatcgc
     3001 cctcggcgat ggtgttcccg ccgagttcca cctcttcggt ggccacgcgc aggttcgcct
     3061 gaacattcgt ctgggcgtac cgcagcagtt cctccacggc tttcggaatg gtgccgggct
     3121 cgcgccggag ccgctcgtac cgctggggtt cgtccagcag gaggtgcgcc atgccggcca
     3181 gccggtccgc ggtggtcccg tagcctccgg cgagcagcac gaggctgaag gagatcagct
     3241 cgtcctggct gagccgatcg tcctcgtcgc gggcggagat cagggtgctc aggaggtcgt
     3301 tgccggggtg gcggcgtttg gcggcgacga gtgcgcccag gtaaccggtc atctctccga
     3361 cagcggcggt gacctcggta tcggagctgg ccgtgaggtt gcgcagcctg tcgcaccagg
     3421 tgtcgagccg gtcgccgtcc tgccgcggga tgcccagcag gtcgcagatg acggccagcg
     3481 gcaaggggcg ggcgagcagc gccaccagat cggcggggcg gtcgtgggcg gcgagggtgc
     3541 tgaggagttc gtcgaccagc agggccacgt cccgccgcat ggcctcggcg cggcggacgg
     3601 tgaaggccgg ggcgaccagc ttgcgcagcc ggctgtgctc cggtgggtcc gcggcgagta
     3661 tcgagtcggg cagggtgggc acgcgtgcga tccgcggccc tgttccctgc gtggcacgtc
     3721 tgcggctgaa acgcggatcg gcaagcacgg tgcgcacgtc ggcgtatcgg gtgaccagcc
     3781 aggccggatc gccgctgggc agccggatgc gggcgcacgg tgcctcgcgg cgcaggtggg
     3841 cgaattccgg cagcgggccg agaccgtcgt gatgggggaa ggggaagtcc acggtgtgag
     3901 ggggcggttc ggggtccgcg tgggtcacga gagctccaag gaaggtgttc ggccgctgag
     3961 ccacagcggt tcgcccgccg ccgggcgggc ggaggctgcg gcggctgacg ggcacgcagg
     4021 cgatcagcgg caggtcgatg gggacggggc gggtcggcgg gggcgggggc gtcagcggag
     4081 ttcgtagacg gagacgtggt aggtgctgtc cggggcgagc gggccgcctc gccaggaacc
     4141 cagacgctgt cgcagccgga gtccggcgcg ctcggccatg agatcgagtt cgcccggtga
     4201 ggcgtagcgg aagtggaccc tgaggtgacg ggtgcggtcc gcctcgtgga tgaggtagtg
     4261 cgactggtac gtctgggtgg agggatcgat ccggcggtgg cagaggatga ggtgctcgtc
     4321 ggtctcggac accacctcgg gtacgcccga tgccgcggcc acggcgaggg cttcgggcag
     4381 atgcgcgtcg aggacgaaga cgccggtggg ttcgaggtgg gtggccacgg ccgtcagaca
     4441 gcgctgttgg gcgtcgcgtc ccgcgagttc gaagaacgtg cctccgacga cgtggacgag
     4501 ggagtacgtg tcggcgaccg gcaggtcggc gaagtcgccc aacgtgacgt ggacgcggtc
     4561 gccgccgggc ttcgagcgga gccgtgccgc catcgctgcc gacgcctcga cgccgtggac
     4621 gtccagaccg cgcgctgcca ggggcaacgc gactctcccg gtgccgatgc cgagttcgag
     4681 gacggggccg tcaccggcga gcgaggcgag ggtgtccacg gtcgccgcgg tgatgcccgg
     4741 cttgccgaac cagcggtcgt agtcgtcggc atggatgtcg gcgtatcccg gagaaggggc
     4801 cgggggctgc gccggagcgt tgcccgggtc gcccgcgttg tcgggggcac tgccggtcgc
     4861 cgccgtgaac gcgggtgcgg ggtggtcgtg gaacgtgggc atggtcgggg tctcctcggc
     4921 ctgcggtcgg ttcggtcccc gtggtgcggg cggaccacgg gaatgggggt gtgcgcgggc
     4981 ctcgcgagcg ctcgggccgg tcagccgaag gggtgcgggc ccggagcggc cggaagatgg
     5041 cgttcgtcgt ccggaccggt gggcagggcg tgggctccgg gggccacgac gcgcaccacg
     5101 gcggagccga ggccccgcgc cggttcgcac gtcgtatcca ccaggatgag gtcctggccg
     5161 gtgtggctgg acagcacggc ggtgccggaa ggatcggcga cggtgtgcga tccgtttccg
     5221 gaagggtcag cgacggtgtg cgatccgttt ccggaaggat cggcgacggt gtgcgacacg
     5281 gttccggaag gatcggcgac ggtgtgtgac tcggttccgg aacggtccgg gccggtgcgc
     5341 ggtccgggtc cgacgcgatc cgggccggcc gatggcggtg gggtttcccg gggcgccagc
     5401 ggggctcgtt ccgtccacag gcgcaggctg tcctgccggt ggtaggccca gagcgcgtgc
     5461 tcgacggcgg agtccggggc gtcgcggccc tgccgacgcg tccaggtgtc ctcggccacc
     5521 ggggtgtgca cgctccagcg caccatgagt gcctcgtacg cggccttctc gatcagcggg
     5581 gcgatcccct ggccgtccgg gccgcaacgg gccccgaacg cctgccgggt gccgtccggt
     5641 gcgtgcaggc atacggcgac gcatcgtgat ccgacgggtg ccgggagatc gagcaccgtc
     5701 gtcgtcacgc cgattccgtc caggagtttc gccagtggtc ccgggagctc acctgcggga
     5761 atcacctggg gagcgcggga aagtccgtac cagcttcggc ggatgaggtc gcgttcgagg
     5821 acctcccacg cggcgtgctg cacggcggcc tccgggtcgg gatgtgccgc gagaccggtg
     5881 gacccggcgc cggggccggg gtcgcagccg gccggcggcc ggtggtgcag aaacacggca
     5941 ccggccggta catgcatgtc cgcaccggtg cggagggagc ggccgcgtac ccagagccgg
     6001 cgggcgtggc ggaagtcgtc cacggtgccg gccgggcctc cggacatcag ttcggcgggg
     6061 tccagggcgg gtatcccctt gcggcgcagt tcgccgtggg tcgccacgat cccggcgtcc
     6121 gactcggcgg cgcggcccgc gaggatgttg ctgacacgtt cgaggagttc accgcgggcg
     6181 ctcgcgacga cgcgcggtgg acggtgaccg gcggcactgc ccaccacgat gggccggttg
     6241 ccggcggcgc agacgggatc gaactgcggc gagcgtgccg cgacccgcgc gaaaacgacc
     6301 tccggccagg cggcgtaggg ggtgaacacc tgtggttccc acggggagac ggacatcagg
     6361 cacgcacccg gaccacctcg gcggcatccc tcaggaccgc ctcaccgtgg tcggtgagcg
     6421 cttcggcatg tcccgtcatg atgtgcacgg catcggtggc ggagcgggcg agctcgtcgt
     6481 ggcgtgcccg cacggccgcg gtcgtgcggg tgtccgccgc ggtctccccg agcaggcgcc
     6541 ggtacagcag gacgctccgg gccaggacca ccatctgctg gaacagcccg gtgagcggcc
     6601 gcgggtcgag gcgcagcggg gtggtgacca gcggcccgcc gtcggccgcg gggcgcagga
     6661 acggctccgc cgccacggag gcggcgttgc accgggtgtg ggtgccctcg tggacgagtg
     6721 cctcggcgag ccggacggtt ccggggagtc cgccggtgtc cggtcccagc cggtcgcgcc
     6781 ggatgaacac ggcgccatgg accgcgaaat ccgtgaagcc gtccagtgcg ggcccgtcca
     6841 gcaggaccgt ctggcgaagg atctccttca gttcggcata cgcgcccggc cagatgtccg
     6901 cgagcagggc gatcgtcccg cggatcacct ccgtatcccc ttcgcgccac gcgacgactg
     6961 ccgagcggct tcggtcgccg atgtcgccgt cggtgcgggg gacggccttc agcgcgcggg
     7021 cgacgctgcg ttcgaggtgg ggttggggcg cggcgagtcc gaccggccag ggcagaccgg
     7081 acggcggtgt gtccgcgatg cccgaccggg cacgggcgag catgccggag aggcgcgcga
     7141 tgccttccgg gcccggaggt ccctttcgca gaacgctttg agcgatgtgt gcggcctcga
     7201 cgaccgcggg gtgcacggcc tcttccgggg agacgtccag cccggcccgt tccagggccg
     7261 accgggtcat acgcgccagt acggaacgtt cttcagcgct ccggcgtgcg tcgggctcca
     7321 ctcgcaccgc gggtacctcc gtgtcgccgt cgggggacgg cgggccctga acggcccgcc
     7381 gcccgaggtg acgtaccgtc agctgggcac gtcgatcatc agtgccagct cgggcagttc
     7441 ctcttcgggg acctgctcga tttcctcgtg catatcgatc tcccttcggc gaccggctcc
     7501 accgacgtcc gacggggagt cccggcggcg ccaaggtacg cacggcaccg ccgtgatcac
     7561 aaggtgcggt cggatcgtca cactgaggac agggagggag ggcggccgac cccgacgaac
     7621 cccgcccggg acgctcccgc tccgtcgcgt caagcccggc aacggccgtc gcgcatcacc
     7681 tccgacctgc ggttcccgct ccgccgacga cggcccctcc acccacggga gtcggccgcc
     7741 caccgcgtcg gccaccacac gggaactggc ctgtccggca ggggaaccag cacagtccag
     7801 ccaatctctg tccagaagag gggaggcggc tccggctcgt ttcgctgcga agaggctcat
     7861 gcgaagaggc tcaaaggatg ccccttcgag gcgcggggta cgttcggcac gggtgcaccc
     7921 ggccgacgac acggggtcgg gggcgtgccg tcgcccgccg gagcccgttg gcaccgctcc
     7981 cgcgcgcacg gcgctgcggg ttcccgccgg gggtgtcccg tcgcgcgtcc ccacgagcga
     8041 atcggaccag ggaaaggctg aaatgccgct cttggaacac cgaccggcac ccgcgatgat
     8101 gacctcgccc gaccggcggc cgtggccacg gcgtttcctc gcacccgacc cgatcgggtg
     8161 gcgcggcccg gcgcgggcga ccgcgtgtcg aagagggagg catggaccga caacaccgtg
     8221 gaaggaccgg cagaggccct cactcactgt ccactcatcg cggcccacac gaggagatgc
     8281 cagtggatcc gacagaccgc tcacttcgcc gccgcctcgc cgcagaccgc gcgcctcgcc
     8341 gcgcccccgt gcgacatcac caccctgccg cagaccccgc gcaacgtcgt acccccgcca
     8401 cagaccccgc gcaacgtcgc agccccgccg cagaccgcgc acttcgccgc gcccccggca
     8461 gggaccgcca ccggtcgccc gcaccatcgg acccgcagga gtaggcacca tgcgcatcag
     8521 ctacctgcat ggcggcagca tcccctccct cttcgccaac ggcgtccacg tcatgcgcat
     8581 gtgcgacgct ttcgccgcag ccggacacga catcaccctc tactccatgc ccggcccgga
     8641 cccggccgag gacccgtaca cctactacgg cgtcagcaac cgcttcccga tccgcctcgt
     8701 cgccgccccc gaccacacac ccaacggata ctggcagcgc gcggacacga tccgccgcat
     8761 ggtggcccgc ggcccccgcc cggacatcgt ctacggacgc gacccctacg ccctgtccgt
     8821 cctctccgat ctggcacctt tcgtgtacga ggtccaccaa ctgcgcaagg acctggtccc
     8881 ggtggggacc gagaggaaac tgctcggcaa gccgggtctg gcccgcgtcg tcgccatcac
     8941 ccatgccctc gcgggcgatc tgcgcaggaa gtacgaagac ctgggcaccc tgccgatcct
     9001 cgtggcaccc gactgcgccg accctcccgg tcccaccgcc acgacgcagg caccgcagct
     9061 gccgggccgc ccggacgtac cgcgggtcgg ctatgtgggg cacctgtacg acggacgcgg
     9121 catcggcctc gtcctcgatc tcgcggaccg ctttcccggt ctcgacttcc acctggtcgg
     9181 aggcacggcc gaggaccggg cccgctggga gaaggcctgc cgggcctccc acgtgtactt
     9241 ccacgggcac cggccgccgt ccgcgctccc ggcctactac ggcctcttcg acatcgtcct
     9301 cgccccctac gagaccaagg tctacacctg gggccggctc ggcgagaccg ggcgctgggc
     9361 ctcccccatg aaggtgttcg agtacatgtc ccacggactc ccgatgatcg cctccgatct
     9421 cccggttctg cgcgaagtcc tccaggacgg ggtcaattgc ctgctccgcc cgcccgagga
     9481 tccggcggcg tgggcggaag ccctcggcga actcgtcgcc gacggcgccc tgcggcgcag
     9541 gctcggcgac gaagcccggc gtcaggtcct ggagcgctac acctggcgcc gccgggccga
     9601 tgacgtcctc gcggacatcg gccacccgga ccgccccggg accggggaag ccgggctccc
     9661 gaggtgacgg agccgggcgc cgcgcagagc cggagcgacg ccgccgcgct ccccggtgcc
     9721 gccgcgtgcg gcgaggcgcc gctgcccacc ggggcgtcgg tggacggata tcccgcacgg
     9781 ccgagcgttc gggccgggga gatcctgcgg ttccatgtct cgacccgggc ggagcggttc
     9841 cgggccgact tctaccgctg cggcgctcgc ccccgtctca tgggcagtac cgcaggaccg
     9901 ggccggtacg cgccccccgg ccgacatgac gaggactggc actggcccgg ccatgacttc
     9961 gcggttcccg gggactggcc ctcgggcgtg tacatcgcgg tgttcggccc cggtagcgcc
    10021 cggcccgccg ccgcgccgcc ggccggtgcc gaggcactcg acgcccggca ctcccggatg
    10081 ctcttcgtgg tgacggcacg cgaacccgca caccgtacgc gcatcctgta caaggtgccc
    10141 gtcttcacct accacgcgta caacaggagc ggcggcgcca gcctgtacgg ggcacttccc
    10201 cacgacgagg cgtaccgcac catcaccctg cggcgccccg ggggcggcat cggcggcccc
    10261 gtgaaagggc agcccgacgc ccacgatccg cggacgccgc ggcagacctt cgcccactgg
    10321 gacgctccct tcatctcctg gctggagacc tcggggcaca ccgtcgacta ctgcaccgac
    10381 ctcgacctcc acgaaagccc ccgacacgcg tccggatacc ggctgttgct ctccgccgga
    10441 catgacgagt actggagtgc gcgcacccgt cggcacatca ccgccttccg tgaccgggga
    10501 ggcaatatcg ccgtcttcgg ggccaacacc tgctggtggc gggtcacacc gaccgccgac
    10561 ggcacggcca tcagatgcgc caagtacccg cccggagtcc cccagggctc ggactgcgac
    10621 cggatgcggg gcgctcccga ccactggtgg gagggtgagc ccgagaactc cctcatcggt
    10681 gtcagctacc gcaacggcgg cggccactgg gccggtctcc gcgaacccct gggcttcacg
    10741 ctccagcaca ccggccaccc cgtcttcgcg ggcaccgggc tgagcgacgg cgatgtcctc
    10801 ggcgaggaac acgcgcttgt gggctacgaa tgcgacggag ccgcgcaccg gcgcgacccc
    10861 ctcggacggg cggtcgcgac cggcacggac ggcacacccg ccgacttcac catcctgggc
    10921 gtggccgacc tccccacggc cccggactcc ggctggtaca ccgccgcccg ggagaacacc
    10981 gacccgggca gggccgcgac cttcggcctc tacaccaggg gcggcacggt cttcaacgcg
    11041 gccaccaccg actgggcgcg gctgctgacc ctcgacccac tggtggacgc catcacccgc
    11101 aatgtgatca gcctgctccg ctgaggcggg gcgggatcgc accgaggagg gcgcactcac
    11161 tccttgaagt tcgcgagcgg gcctccgcgc cggcaactcg gcctgccgta cggctcgacg
    11221 gctcgacggc tccagtatcg acgttctccg aaccctcaga ccaggagtgg tgggcatgaa
    11281 gatggtgatc accgggggcg ccggattcat cggcagcaac ctcgcacacg cgttgacgcg
    11341 gcgacccgag gtgtcc
//
