LOCUS       NZ_LWLE01000036        11125 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold7, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000036
VERSION     NZ_LWLE01000036.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 480808)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480808)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 279657
            Orig. end                         :: 290782
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11125
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11125
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11125
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6125](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6125)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(425..943)
                     /locus_tag="A6A29_RS38110"
                     /old_locus_tag="A6A29_20500"
     CDS             complement(425..943)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975090.1"
                     /locus_tag="A6A29_RS38110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20500"
                     /product="FHA domain-containing protein"
                     /protein_id="WP_073727797.1"
                     /transl_table=11
                     /translation="MSELTLTVMRLGFLAVLWLFVIVAVQVIRSDLFGTRVTQRGSRRT
                     AADARPQQARQQQQTAPPPQRQQPGRQRRGAPTKLVVSEGTLTGTTVALQGQTITLGRA
                     HDSTIVLDDDYASSRHARIYPDRDGQWIVEDLGSTNGTYLDRTRLTTPTPVPLGAPIRI
                     GKTVIELRK"
     gene            complement(954..1829)
                     /locus_tag="A6A29_RS38115"
                     /old_locus_tag="A6A29_20505"
     CDS             complement(954..1829)
                     /GO_function="GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975091.1"
                     /locus_tag="A6A29_RS38115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20505"
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="WP_073727799.1"
                     /transl_table=11
                     /translation="MGVMKRFEQRLEGLVNGTFAKVFKSEVQPVEIAGALQRECDNNAT
                     IWNRERTVVPNDFIVELSPPDYERLSPYSGQLGDELSGLVRDYAKQQRYTFMGPIKVHL
                     EKADDLDTGLYRVRSRTLASSSSQQDPQGQHGGPASAQPQRPGRPAAPQGTGGYGYPPS
                     AAPPMPAAPPPGTGRPPGTSTDRRPPTAPGPLPDAQVRRWIEINGTRHQISRPTLVMGR
                     STDADVRIDDPGVSRRHCEIRTGTPSTIQDLGSTNGIVVDGQHTTRATLRDGSRIVVGS
                     TTIVYRQAEG"
     misc_feature    complement(1783..1798)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.32"
                     /tool="antismash"
     tRNA            2145..2227
                     /anticodon=(pos:281835..281837,aa:Leu,seq:cag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="A6A29_RS38120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="A6A29_20510"
                     /product="tRNA-Leu"
     gene            2145..2227
                     /locus_tag="A6A29_RS38120"
                     /old_locus_tag="A6A29_20510"
     misc_feature    2379..2393
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.41"
                     /tool="antismash"
     gene            2441..3160
                     /locus_tag="A6A29_RS38125"
                     /old_locus_tag="A6A29_20515"
     CDS             2441..3160
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982992.1"
                     /locus_tag="A6A29_RS38125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20515"
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_079183190.1"
                     /transl_table=11
                     /translation="MYIDTAHSITAHSATGPGGTALGVTGFSGTAYPEAAGPPQKAGPD
                     YGVGVYRGITEWAAGTPEWLQKLGEVVTEGGILVLVALFVLQWWRARRGDDRTLALALL
                     GPVAVAVVYALSEIIKVWLHEERPCRDIPGVTAIAECPEPGDWSFPSNHSVIAAASAMA
                     LVVSWRVIGLLVVSLAVLVALSRVYVGVHYLHDVAAGFLLGAVFAPLLMVVLAVPGTRA
                     VSRFRRARGRRGTGIAA"
     gene            complement(3336..4013)
                     /locus_tag="A6A29_RS38130"
                     /old_locus_tag="A6A29_20520"
     CDS             complement(3336..4013)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961898.1"
                     /locus_tag="A6A29_RS38130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20520"
                     /product="response regulator transcription factor"
                     /protein_id="WP_274535856.1"
                     /transl_table=11
                     /translation="MTTVKLILADDETMIRAGLRAILAADPAIEVVAEAADGREAVALT
                     LEHRPDVVLMDIRMPVMDGLTATEEIKRAAPGTSVLVLTTFSEDEYVSRALGGGASGFM
                     LKSGDPRELLAGVHTAAAGASCLSAKVAQHVIAELNRGSARHRDVRLASLHERVTALTA
                     REQEVLALLGAGLSNAEIGRRIHVVEGTVKSYVSAVFTGLGVRNRVQAAIVAYESGLIG
                     RED"
     gene            complement(4027..5004)
                     /locus_tag="A6A29_RS38135"
                     /old_locus_tag="A6A29_20525"
     CDS             complement(4027..5004)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (64-171). E-value:
                     7.6e-08. Score: 23.7. Matches aSDomain:
                     nrpspksdomains_A6A29_RS38135_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="A6A29_RS38135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20525"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_073727803.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.1e-15, bitscore:
                     52.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGPGGILVAGGTGFIGSAVVRVLSRHLPAPPAPLRALARRHPDP
                     ARTGPVAPGVEYLIADLTDPASLHGTCDQVDTLLHCASYVGPDDSACSAVNDAGTRALL
                     DEAARAGVRRIIHISTTAVYGAGPHRGLSEGTRPPAPASEVSRTRLLAEQAVLGAAGTV
                     LRPPFVYGRGDAWVVPAIAELLHRVPALPDGGTALISLVAVEDLARLVTSLALSPAPPP
                     PGVHHAGHPEPVALRDLVTALARTAGLPLPTASLPTPEYLERLGRTPGAVSVRQARMMT
                     EDRWYRSPLWESTGCSSGPGLAARLSGHGEWYRNVLSRTAPVPR"
     aSDomain        complement(4492..4812)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A29_RS38135_NAD_binding_4.1"
                     /evalue="7.60E-08"
                     /label="A6A29_RS38135_NAD_binding_4.1"
                     /locus_tag="A6A29_RS38135"
                     /protein_end="171"
                     /protein_start="64"
                     /score="23.7"
                     /tool="antismash"
                     /translation="PASLHGTCDQVDTLLHCASYVGPDDSACSAVNDAGTRALLDEAAR
                     AGVRRIIHISTTAVYGAGPHRGLSEGTRPPAPASEVSRTRLLAEQAVLGAAGTVLRPPF
                     VYG"
     gene            complement(5001..6125)
                     /locus_tag="A6A29_RS38140"
                     /old_locus_tag="A6A29_20530"
     CDS             complement(5001..6125)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="A6A29_RS38140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20530"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_073727805.1"
                     /sec_met_domain="AfsA (E-value: 4.7e-21, bitscore: 69.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALAAPADQNIRTRTPLTGEPSVMSPVPPHLVHRSDEADVLLTH
                     FGRADADTYLFTVQWPTTHALFSSVLGQVNPMLIAETVRQAGTVLAHKVFEAPMDDQFL
                     LWNLRYESFHDLRRPGGPPTPTKAVIRSPEVTLRGRRLATIHIDMELFSGHLRIGTGAA
                     SANCLSAAAYRRLRPGLPDAPDTSDAHDAPVPAVAPRTAILPSLVGKENEQDVMLSPLD
                     STSPLDTAPAHTPAAPLDTASAAPGAPWSAARPAGAPRTAAWRLRADRDHPVIFDSQSD
                     HLPGRGIIEAMRQAAQLAAGLDHALVLSMNAEFRRYVELDRPCTVSTEIGAEQPDGRIP
                     IEVTMYQDNEVAALALLLVQDVKPAARHNLPVRP"
     misc_feature    complement(6175..6189)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     misc_feature    complement(6195..6213)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.58"
                     /tool="antismash"
     misc_feature    complement(6199..6212)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.79"
                     /tool="antismash"
     gene            6526..7254
                     /locus_tag="A6A29_RS38145"
                     /old_locus_tag="A6A29_20535"
     CDS             6526..7254
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006125953.1"
                     /locus_tag="A6A29_RS38145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20535"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_079183192.1"
                     /transl_table=11
                     /translation="MAKQARAVQTRRSIVEAAASVFDDYGYERAAISEILRRAKVTKGA
                     LYFHFASKEAIAQAIMDEQTATVDLVEDGSPLQSLVDGGQQFAFALRHDSMARAGTRLS
                     IEGVFLGGPHPWSDWIAATCRMLELGKERGEVLPQVDPLVSAEVIVASFTGIQLISEAN
                     SGRADLRERVAQMWRHILPSIAHPGVIVHIKPEGRVDLAAQALERERQEAEESAAEAAE
                     AAPVGAAVGEAVGASVGAAG"
     gene            complement(7458..8069)
                     /locus_tag="A6A29_RS38150"
                     /old_locus_tag="A6A29_20540"
     CDS             complement(7458..8069)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488998.1"
                     /locus_tag="A6A29_RS38150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20540"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_073727807.1"
                     /transl_table=11
                     /translation="MATLLHLDSAVFPEGSASRDITSAFVETWREQHPEGKVVYRDLAA
                     APLPHLDAAAVAAGADDPLRRELADELAAADAVLIGAPMYNFSIPSTLKTWLDQVIIVG
                     HNAGSDSPVAGVPVTVVASRGGSYAPGTPREDFEFVTNYLEKVLTGMFSVEVDFIVPEL
                     TLAHSKPEMAELIPLAVESRAKALEDAAQKAKALASRLAA"
     gene            8262..8609
                     /locus_tag="A6A29_RS38155"
                     /old_locus_tag="A6A29_20545"
     CDS             8262..8609
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155252.1"
                     /locus_tag="A6A29_RS38155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20545"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_073727809.1"
                     /transl_table=11
                     /translation="MADHSEQTCRRVDMGITRVFELFGKRWTGPIVSVLMRQSVHFADL
                     RRAIPGISERMLSDRLSELGAAGLVVREVDEGPPLRVSYRLTDAGAAMEPALKELGRWA
                     DVYLPGGDSSC"
     gene            complement(8720..9169)
                     /locus_tag="A6A29_RS38160"
                     /old_locus_tag="A6A29_20550"
     CDS             complement(8720..9169)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155253.1"
                     /locus_tag="A6A29_RS38160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20550"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_073727812.1"
                     /transl_table=11
                     /translation="MPGTPGEREKSLDIIQRELTAFARRARAAAARLHPELPLVSYTLL
                     AHIDDQHGCRATDLAAHYMLDKSTISRQIATLEKLGLVERHPDPTDHRIQVLHPTEAGT
                     QALASTQASRRAAYQERLADWTAEDLTRFADYLLRYNSTGGPAPQ"
     gene            9251..10501
                     /locus_tag="A6A29_RS38165"
                     /old_locus_tag="A6A29_20555"
     CDS             9251..10501
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570850.1"
                     /locus_tag="A6A29_RS38165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_20555"
                     /product="hypothetical protein"
                     /protein_id="WP_241838795.1"
                     /transl_table=11
                     /translation="MSAGPSTASGATAGALRHVLTHLVTPLLMCVGMGLAYLGAFHAPE
                     PHDLRVDVVGSGPSVQVLAQTLQDTGDGALDVRTVADRDAAVNHLETQASYGAYVPGRS
                     PELLVATASSDTSATVVQKVFTEVAAGQGAPLKVTNVAPTADGDPTGQGIFFLLVAISI
                     GSYASVAVIGGAGGALALRIRAALVAGASLVVSLIGAVFAGPLFGLVDHGLGGLWAMAW
                     LYSAGILLVGVGLHTFLKRWTTLGVMVLFVMLNFTSSGGIFRPELQPGFFGSLHAFWNG
                     AGFVEGVRSHVYFDGHALGGHVLVLVLWFVAGLAVMGVAGLAEARKRAAAQAESGVGAG
                     TGTPTGTGAGATTETGTGTSSGTRADAGPAESGSESVSESGSRKGSGSDSGSVAGVSRT
                     GEQREELEEELEEAVGV"
ORIGIN
        1 tgagcgtggt gcccatgccc tccagctggg ggtcctcctc gaccatcatg cgcagctggt
       61 cgttggcccg ctggaccgcc gtaccgagcg aggtgaggat gtcggagccg ggtacgtcgt
      121 catcgagctg gacgagggtg gagatcacct cggagctggc gacctcaccg gccgcctggc
      181 cgcccatgcc gtcggcgatg gcgagaaggc gggggccggc gtagccggag tcctcgttgc
      241 cttcccggat catgcccttg tgcgatccgg cggcgaagcg cagggacaga ctcatgcgca
      301 cctcgcccgt cggctcgggg tacagccggt ctcgagccac actgcccacc ctccggtcgg
      361 gagccgggca gcgtccgtcg tcaggaccgc cgcggctcgc tcgctccgct cgctcattgt
      421 cgtactactt ccgcagctcg atgacggtct tgccgatacg gatcggcgcg cccagcggaa
      481 caggtgtcgg ggtggtgagt cgggtccggt cgagatacgt gccgttggtg gacccgagat
      541 cctcgacgat ccactggccg tcacggtccg ggtagatcct ggcatgcctg ctggacgcgt
      601 agtcgtcatc cagcacgatc gtcgaatcgt gcgcgcggcc cagggtgatg gtctggccct
      661 ggagcgcgac cgtggtgccc gtgagggtgc cctcggagac gacgagcttg gtgggtgccc
      721 cgcggcgctg gcgcccgggt tgctggcgct gcggtggcgg cgccgtctgt tgttgttggc
      781 gtgcctgttg cgggcgcgcg tcggcggcag tgcggcgtga gccgcgctgc gtgacgcgcg
      841 ttccgaacag gtcgctgcgg atgacctgga cggccacgat cacgaacagc cacagaacag
      901 ccaggaaacc tagccgcatg accgtcaggg tcagctctga cattgccccc gcttcaccct
      961 tcggcttgcc ggtaaacgat ggtggtgcta cccacgacga tccgcgagcc gtcgcggagc
     1021 gtagcgcggg ttgtgtgctg cccgtctacc acgatgccgt tggtagaccc gagatcctgg
     1081 atcgtcgagg gcgttccggt ccggatctcg cagtgccgac gcgatacgcc gggatcgtcg
     1141 atccgcacgt cggcgtcggt gctgcgtccc atcaccagcg tcggacggga gatctgatgg
     1201 cgggtgccat tgatctcgat ccagcgccgc acctgggcgt ccggcagggg gccgggcgcg
     1261 gtcggcgggc gccggtcggt cgaggtgccc gggggccgtc cggtgccggg cggcggggcc
     1321 gcgggcatcg gcggagcggc gctcgggggg tagccgtagc cgccggtgcc ctgcggggcg
     1381 gcgggccgcc cgggtcgctg cggctgggcg gacgcggggc cgccgtgctg gccctgcggg
     1441 tcctgctgtg acgaactcga cgccagggtg cggctgcgta cccggtagag cccggtgtcg
     1501 aggtcgtcgg ccttctccag atggaccttg atcgggccca tgaaggtgta gcgctgctgc
     1561 ttggcgtagt cgcgtacgag gccggagagc tcgtcgccga gctggcccga gtacgggctg
     1621 agccgctcgt agtcgggcgg gctgagctcg acgatgaagt cgttcgggac gaccgtccgc
     1681 tcacggttcc agatcgtcgc gttgttgtcg cactcacgct ggagggcacc cgcgatctcg
     1741 accggctgca cctcggactt gaagactttg gcgaaggtgc cattgaccag accttcgaga
     1801 cgctgctcga aacgcttcat gactcccatg gggcacctcc tccggtgtcg tcgtccctgt
     1861 actgcttact gatcgtatcc acgcgtcggg aaatcggctg gttccccttg tctgcccagt
     1921 ggatgagtgt cacccctcac acggatcgta gaggtggctc cctgacagtg tcccgcacac
     1981 ggggtgctct ctgaaggagt gggtgcgccc gctccgcgtt cccgcttccc cacctgggat
     2041 cggcgaaaca acggatgtga atccaccctg tccagcgtgc taatcttccg gatgtcgcca
     2101 ggagctcgca ccacacggtg ggagagcccg gaaacaccac tcatgcgcga gtggcggaac
     2161 ggcagacgcg ctggcttcag gtgccagtgt ccttagggac gtgggggttc aaatcccccc
     2221 tcgcgcacaa agagagcccc cgcttcggag agatccggag cgggggcttc ttcgttgtac
     2281 ggctgtgatc ttgccgccgc cctgacgaaa gtcggggtgg tgccctgccg gaagaggggg
     2341 tgaccggttc cttcggtggg atgtgtccgc atccggccgg accttacgtt ccgaatgacc
     2401 ggagaacgtg ggaatcggcg tgcggagaac gacacccctt atgtacatcg acacagcgca
     2461 cagcatcacg gcgcacagcg ctacagggcc cggcggtacg gcgctcggcg tcaccgggtt
     2521 cagcggtacg gcctatccgg aagcggccgg tccgccgcag aaggcgggac ccgactacgg
     2581 agtcggggtg taccgcggga tcacggagtg ggcggcaggg actcccgagt ggctgcagaa
     2641 gctgggggag gtcgtcaccg agggcgggat actcgtcctc gtcgccctct tcgtcctcca
     2701 gtggtggagg gcccgcaggg gcgacgaccg gaccctggca ctggccctgc tcggtccggt
     2761 ggcggtggcg gtggtctacg cgctgagcga gatcatcaag gtctggctgc acgaggagcg
     2821 cccctgccgg gacattccgg gtgtcacggc gatcgcggag tgcccggagc cgggtgactg
     2881 gtcgttcccc agcaaccatt ccgtcatcgc ggccgcgtcg gcgatggccc tggtcgtctc
     2941 ctggcgcgtc atcggactgc tggtggtgtc tctggcggtg ctggtcgcgc tgtcccgcgt
     3001 gtacgtcggc gtccactacc tgcacgacgt ggcggccggc ttcctgctcg gtgcggtgtt
     3061 cgcgccgctg ctgatggtgg tcctggcggt gcccggaacc cgggcggtga gcaggttccg
     3121 cagggcccgc ggacggcggg gcacggggat cgcggcgtga gccgaaggac ggccgccgcc
     3181 ggggctccgg cggcggagtg cctgggcgcc ggggctgcgg cggcggtgcc tgggcggtga
     3241 ggtgccggtg tcagggttcc ggggtggcgg tgtgtcaggg ctctggggcg gcgggggcgc
     3301 cggtgtgtga ggggctccgg ggtggcgcgg ggcgttcagt cctcgcgtcc gatcagcccg
     3361 gactcgtacg cgacgatggc ggcctggacc cggttgcgta ccccgagacc ggtgaagacg
     3421 gcgctcacat agctcttcac ggtgccctcc acgacgtgga tccgccggcc gatctcggcg
     3481 ttggacagcc cggcgcccag cagggcgagc acttcctgtt cccgcgctgt cagcgcggtg
     3541 acccgctcgt gcagtgacgc caggcgtacg tcgcggtgcc gggccgagcc gcggttcagc
     3601 tccgcgatga cgtgctgtgc caccttcgcg gagaggcagg aggccccggc cgcggcggtg
     3661 tgcacgccgg ccagcagctc gcgcgggtca ccggacttga gcatgaagcc gctggcgccg
     3721 ccgccgagag cccggctcac gtactcgtcc tcggagaacg tcgtcagcac gagtacggag
     3781 gtgccgggcg cggcacgttt gatctcctcg gtggcggtga gaccgtccat gaccggcatg
     3841 cggatgtcca tcaggacgac gtccggccgg tgttccaggg tcagcgcgac ggcctcccgc
     3901 ccgtccgccg cctcggccac gacctcgatg gcggggtcgg cggcgaggat ggcccgcaga
     3961 ccggcccgga tcatcgtctc gtcgtcggcc aggatgagct tgacggtggt gatggatggc
     4021 tcctcttcag cggggcacgg gggccgtgcg ggacaggacg ttccggtacc actcgccgtg
     4081 tccgctcagc cgtgccgcga gtcccgggcc gctggagcag ccggtgctct cccacagagg
     4141 ggaccggtac cagcggtcct ccgtcatcat ccgggcctgg cggacgctga cggccccggg
     4201 ggtccgtccg agccgttcca ggtactcggg cgtcggcagg ctcgccgtgg gcagcggcag
     4261 tcccgcggta cgggcgagag ccgtgaccag gtcgcgcagg gcgaccggtt ccgggtggcc
     4321 cgcgtggtgg acgccgggtg gcggcggggc gggggacagc gccagcgagg tcaccagccg
     4381 ggccaggtcc tcgaccgcca cgagcgagat cagggcggtg ccgccgtcgg gcagcgcggg
     4441 gacccggtgc aggagctcgg cgatggccgg gaccacccag gcgtcgcccc gcccgtacac
     4501 gaacgggggc cgcaggaccg ttcctgcggc gcccagcacg gcctgttcgg ccagcagcct
     4561 ggtgcggctg acctcggacg cgggggcggg tggccgcgtg ccctcggaca ggccgcggtg
     4621 cgggccggct ccgtagacgg cggtggtgct gatgtggatg atccggcgga ccccggcgcg
     4681 ggccgcctcg tcgagcaggg cccgggtgcc ggcgtcgttg accgcgctgc aggcggagtc
     4741 gtccggaccc acgtaggacg cgcagtgcag gagcgtgtcc acctggtcac aggtgccgtg
     4801 cagcgaggcg gggtcggtga ggtccgcgat gaggtactcg acgccgggag ccaccgggcc
     4861 ggtgcgagcg gggtccggat ggcgtcgggc cagcgccctc aacggggcgg ggggtgcggg
     4921 caggtggcgg ctcaacaccc gcacgacagc ggagccgatg aatccggtgc ccccggccac
     4981 caggatcccg ccagggcccg tcatgggcgt acgggaaggt tgtggcgggc tgccggtttg
     5041 acgtcctgca cgagcaggag ggcgagggcg gcgacctcat tgtcctgata catggtcact
     5101 tctatcggta tccggccatc gggctgctcc gcaccgatct ccgtgctgac ggtgcagggg
     5161 cggtcgagtt cgacgtaccg ccggaactcc gcgttcatgg acagcacgag ggcgtggtcg
     5221 aggcccgcgg cgagctgggc tgcctggcgc atggcttcga tgatgccccg gccgggcagg
     5281 tggtcgctct ggctgtcgaa gatgacgggg tggtcccggt cggcgcgcag ccgccaggcg
     5341 gcggtacggg gggcgccggc gggccgtgcg gcgctccagg gggcgccagg ggcggcggac
     5401 gcggtgtcca ggggtgccgc gggggtgtgc gcgggcgcgg tgtccagggg ggacgtggag
     5461 tcgagggggg acagcatgac gtcctgttcg ttctccttgc ccacgaggga gggcaggatc
     5521 gcggtccgtg gtgcgacggc cggcacggga gcgtcatgtg cgtccgatgt gtccggtgcg
     5581 tccgggagtc cggggcgcag gcgtcgatag gcggcggcgg aaaggcagtt cgcggaggcc
     5641 gcgcccgtgc cgatccgcag atggccggag aacagctcca tgtctatgtg gatcgtggcc
     5701 agtcggcggc cgcgcagggt gacctccggg ctgcgtatga ccgctttcgt aggggtcggt
     5761 ggcccgccgg gccggcggag atcgtggaag gactcgtagc gcaggttcca gagcaggaac
     5821 tggtcgtcca tcggcgcctc gaagaccttg tgcgccagta cggtgcccgc ctgccggact
     5881 gtctccgcta tcagcatggg gttgacctgt cccagaactg agctgaacag cgcgtgtgtc
     5941 gttggccatt ggacggtgaa cagatacgtg tcagcatcgg ctcggccgaa gtgcgtcagc
     6001 aggacatcgg cctcgtcgga ccggtgcacg aggtgcggcg gcacgggact catgacggac
     6061 ggctcaccgg tgaggggtgt gcgggtgcgt atgttctggt ctgccggagc ggcgagtgcg
     6121 gtcacgtgat ctgtcccctt tcacgtcgta ggagccggat ggctcccacg gccctgcaaa
     6181 tcaaaaccat ctggccggtt tatttatgca atgagtctgc tcgcgagtgg tagttgatgc
     6241 tatgggagaa agcgctcaac ttgcgtgctg acaagtgcgc gaaccccttg accaaccgga
     6301 acaagagggt ttttcacacg tcagaagggg taggggggca gcgcgttggg cgacgcctcg
     6361 gcatactgcg acaacgtgcg ttggacgcct atggtccagt gcgtaaacag accgttcgtc
     6421 ggttagagtg aagtgcttgc atacgctgac tactgatgct ggattgacgg ttgctcggtt
     6481 gctcggcgcg acggccgagc acacagaacg tcccggaggc aagagatggc gaagcaggct
     6541 cgcgcggtgc agacgaggcg ttcgatcgtg gaggcagcgg cgagtgtctt cgacgactac
     6601 gggtacgagc gtgccgcgat ctcggagatc ctccgccgtg caaaggtcac caaaggggct
     6661 ttgtacttcc acttcgcgtc caaggaagcc atcgcccagg cgatcatgga tgagcagacg
     6721 gccacggtgg acctggtgga ggatggttct ccgctccagt cactggtgga cggcggtcag
     6781 cagttcgcct tcgcgctgcg ccacgactcg atggcgcgtg ccggtacccg tctctccatc
     6841 gagggtgtat tcctcggcgg tccgcatcca tggagcgact ggatcgcggc gacgtgccgg
     6901 atgctggaat tgggcaagga gcgcggagag gtgctccccc aggtggaccc actggtttcg
     6961 gccgaagtca tcgtcgcgtc gttcaccggt attcagctga tttcggaggc caattccgga
     7021 cgggccgacc tgcgtgagcg cgtggcccag atgtggcgcc atattctgcc gtccatcgcc
     7081 catcccggtg tcatcgtgca tatcaagccg gagggccggg tggacctggc cgcgcaggcg
     7141 cttgagagag agcggcagga ggccgaggag agcgcggcgg aggcggcgga ggcagcccct
     7201 gtgggtgccg ctgtcgggga agccgtcggg gcgtcggtcg gcgcggcagg ctgagcggtc
     7261 ggctgagcct tcttgagggc gccctgtctg agggcgcggt gcttcgtttc acgtgaaacg
     7321 ccccgaatcg cgagggtgca cagggttgta gacacacagg gctgcggggc tcattcgagc
     7381 cccgcagccc tgttctgtca gtcgcgcggg ccgctgggcg gcggcgcgct cgcgccgctg
     7441 ggcccgcggt cgcggtgtca ggcggccagt cggctcgcga gggcttttgc cttctgcgcg
     7501 gcatcttcga gagccttggc gcgggactcc acggcgagcg gtatcagctc ggccatctcg
     7561 ggcttggagt gcgccagcgt cagctccgga acgatgaagt cgacctcgac ggagaacatt
     7621 cctgtcagca ccttctccag gtagttcgtg acgaactcga agtcctcgcg cggggtgccg
     7681 ggggcgtacg aaccgccacg gctcgcgacg acggtgacag ggacccctgc gacagggctg
     7741 tcggatccgg cgttgtggcc cacgatgatc acttggtcca gccaggtctt cagtgtggac
     7801 gggatggaga agttgtacat gggcgcgccg atcaggacgg catccgcggc ggccagctca
     7861 tccgccagct cccgtcgcag cgggtcatcg gcaccggcgg ccactgccgc ggcgtccaga
     7921 tggggaagcg gtgcggcggc gaggtcccgg tagacgacct tgccctccgg gtgctgttcg
     7981 cgccaggtct ccacgaacgc cgaggtgatg tcgcgggagg cggaaccctc ggggaagacg
     8041 gcggagtcga ggtgcagaag tgtggccatg gaggtctcca caggtatgag ggatcgagag
     8101 gcgcgggccg ggttcgtcgt cggccatccg ccgcgagtgg cgcgggcggg tggtgcggtg
     8161 gcggacccgc cgaccggacg ctaatttttc gtgtgtagct aggaatacca tagtccctta
     8221 ctttttgtaa gtacctcacg agggcgcagt actctggggg tatggcagat cacagcgagc
     8281 agacctgtcg gcgggtcgac atgggcatca cccgcgtctt cgagctgttc ggcaagcgct
     8341 ggaccggtcc gatcgtttcg gtgctgatgc ggcagtcggt gcacttcgcg gatctgcggc
     8401 gcgcgattcc gggcatcagt gagcgcatgc tctcggaccg gctgtccgag ctgggggctg
     8461 ccggtctggt ggtgcgtgag gtcgacgaag ggccgccgct gcgcgtctcg taccgcctga
     8521 cggatgccgg ggccgcgatg gagccggccc tcaaggagct cgggcgctgg gcggacgtat
     8581 atctgccggg cggggattcc agctgctgac ggctccgctg cgggggcgcg ccgggagccc
     8641 gccgggggtg cctgctgcgg agcccgccgg gggtgcctgg gctgcctgct gcggagatgt
     8701 tcgggatgcc cgtgatgccc tactgcggag ctgggccgcc ggtcgagttg tagcgcagca
     8761 ggtagtccgc gaaccgggtg agatcttccg cggtccagtc ggccagccgc tcctgatagg
     8821 cggcccgacg gctcgcctgg gtggaggcga gggcctgtgt accggcttcg gtggggtgca
     8881 gcacctggat gcggtgatcg gtggggtccg ggtggcgttc gaccaggccg agcttctcca
     8941 gggtcgcgat ctgccggctg atggttgatt tgtcgagcat gtagtgagcg gccaggtcgg
     9001 tcgcgcggca gccgtgctgg tcgtcgatgt gtgcgagcaa ggtgtacgac acgagcggca
     9061 gctcggggtg gagccgtgcc gccgccgcgc gcgcacgccg cgcgaaggcg gtcagctcac
     9121 gctggatgat gtccagcgac ttctcgcgct ctccgggggt gcctggcacg gattcacctt
     9181 cctcgaaatt tcagttgtat agtacaacgg aactaggagt tgtaaaagcc aaccattgga
     9241 gatcgtccgg atgtccgcag gcccgagtac cgcttccggc gccaccgccg gtgcgctgcg
     9301 tcatgtgctg acccacctcg tcaccccgct gctgatgtgc gtcggcatgg ggctcgctta
     9361 tctgggggcc ttccacgccc cggaaccaca cgatctgcgc gtggacgtgg tcggctccgg
     9421 gccgagcgtc caggtgctgg cgcagaccct gcaggacacg ggggacggcg cactggacgt
     9481 gcgtaccgtc gccgatcgcg atgcggccgt gaaccacctg gagacacagg cgagttacgg
     9541 tgcgtacgtg ccggggagga gccccgaact gctggtggcc accgcctcgt cggacaccag
     9601 cgccacggtg gtgcagaagg tcttcaccga ggtcgcggcc ggccagggcg ccccgctgaa
     9661 ggtgacgaat gtggctccga cggcggacgg cgatccgacc gggcagggca tcttcttcct
     9721 gctggtcgcg atcagcatcg ggtcgtacgc gtcggtcgcg gtgatcggcg gcgcgggcgg
     9781 tgcgctggcg ctccggatca gggccgcgct ggtggcgggc gcctcgctcg tggtgagcct
     9841 catcggcgct gtgttcgcgg ggccgctgtt cggtctcgtg gaccacgggc taggaggtct
     9901 gtgggcgatg gcgtggctct actccgcggg aatcctgctc gttggtgtcg gactgcacac
     9961 cttcctcaag cgttggacga cgctcggggt gatggtgctg ttcgtcatgc tcaacttcac
    10021 cagctccggc ggtatcttcc ggcccgagtt gcagcccggc ttcttcggct ccctccacgc
    10081 cttctggaac ggcgcgggct tcgtcgaggg tgtgcggagc catgtctact tcgacggtca
    10141 tgcgctcggc gggcatgtgc tggtgctcgt gctgtggttc gtggcgggtc tggccgtgat
    10201 gggagtcgcg ggtctggccg aggcccggaa gcgggcagct gctcaggcgg agtcaggggt
    10261 gggtgcaggt acggggacgc ctacggggac gggggcgggg gcgactacgg agacggggac
    10321 ggggacgagt tcggggacgc gggcggacgc cgggcctgcg gagtccggtt ccgagtccgt
    10381 ctccgagtcc ggttccagga aaggttccgg gagtgattcc gggagcgtgg ccggggtgtc
    10441 ccgcacgggg gagcagcgcg aggagctgga ggaagagctg gaggaggcgg tcggcgtctg
    10501 acctggacag tgagttgtcc acaggccgag tcgcgggttg tccacagggg cggacgggtt
    10561 tcggggaacg gcggtaccgt cgtggtcgat tgatggtggg acgacagccg gacaagggtg
    10621 ggggaggcgg cgcgccatgg gcgaggtcga tgcgacaagg tcggcacagc gggctgtgat
    10681 ggcggacctg cgtattccgg aggtccccgg gttcgcccgg cgctcggcgc cgaacccgcc
    10741 gaaggccgcc ggtaaggcgt tgcgggaccg ggtgccgcgc tcctcgcacg atgcgctggt
    10801 ccttccgccg gggcggccgg acgcggtgcg ggcggtcgag gagtcgaacc tggggcgggt
    10861 gccacacctg acgccgatcc gggtggggcg gatggcggcc acgccgttcg cgtttctgcg
    10921 gggttccgcc gggctcatgg cccacgacct gatgggcacg ccggtcaccg gtgtgggtgc
    10981 ccagctctgc ggcgacgcgc atgccgccaa cttcggtctt tacggcgatg cccggggcag
    11041 cctggtcatc gatctcaacg atttcgacga gaccgtcttc ggcccctggg aatgggacct
    11101 caagcggctc gccacctctc tggtg
//
