LOCUS       NZ_LWLE01000012        10405 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold2, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000012
VERSION     NZ_LWLE01000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 710327)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710327)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 650426
            Orig. end                         :: 660831
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="5"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    292..306
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    2413..2426
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.65"
                     /tool="antismash"
     gene            2833..3795
                     /locus_tag="A6A29_RS20400"
                     /old_locus_tag="A6A29_08980"
     CDS             2833..3795
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528941.1"
                     /locus_tag="A6A29_RS20400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_08980"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_073721819.1"
                     /transl_table=11
                     /translation="MTPETPQLTAAGIDPFDESVYRAVLSRRTAAPAELTADLCCSAER
                     VARALDRLRDHGLVGRLSGSRRRYAAIEPGAAVEALVRARSNELERVRSAADELSRLFA
                     AARTGATYEDEVEITTGSEALGRWFVRLQQEAHEDVMTLDRPPYALTTSNPVEATALGR
                     GVRYRAVYAPEALEWPGVLDDIRELVRHGEQARVLPGLGIKLAIADRRLALMPLSLDLK
                     NVRAAVIRPSTLLDALTDYWEMCWERALPLNAPAEDPLGEEDRLVLTLLVSGLKDEAIA
                     RQLGWSVRTMRRRISRLHDLLGAANRFQAGVIAARRGWL"
     gene            complement(<3959..>4107)
                     /locus_tag="A6A29_RS46725"
                     /pseudo=""
     CDS             complement(<3959..4107)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104928.1"
                     /locus_tag="A6A29_RS46725"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="ThuA domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MMGLHPAACTEYHWPYHGELLGARFAAATPPSSRAPYWSRTGAIR
                     RCAT"
     misc_feature    4979..4994
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.24"
                     /tool="antismash"
     gene            5001..5405
                     /locus_tag="A6A29_RS20410"
                     /old_locus_tag="A6A29_08990"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966560.1"
                     /locus_tag="A6A29_RS20410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_08990"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_073721658.1"
                     /sec_met_domain="melC (E-value: 9.2e-41, bitscore: 134.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLTRRQTLGAVTGAAAGIALVGVATASADTAPVKGNDKAAAPG
                     GTGGPASFDEVFQGRRIQGLATEAHAHAGHSTGYRVLVDGRELHVMQHGKSGWSSAINH
                     YERFANPRDAARTAVTSLKGADVVPFDPTA"
     gene            5422..6246
                     /locus_tag="A6A29_RS20415"
                     /old_locus_tag="A6A29_08995"
     CDS             5422..6246
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379204.1"
                     /locus_tag="A6A29_RS20415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_08995"
                     /product="tyrosinase family protein"
                     /protein_id="WP_073721660.1"
                     /transl_table=11
                     /translation="MTVRKNQANLTGAEKKAFVDAVLEVKRKGVYDEFVAAHRLRFALD
                     MNTGIYVGHFGPSFLPWHRKFLIDFENELQKVDPSVSIPYWDWTADNTVGSSLWAPDFL
                     GGTGRALDDQVMDGPFAYSSGKWPITIQVDTRPFLARNLAYRVPTLPTRAEVDAALAIP
                     TYDSAPWRDGSSGFRSTLEGTAGYMSMHNRVHTWIWGQMETSVSPNDPVFWLHHSFVDK
                     LWADWHTLHPSSAEYLPAGGTPDVIDLDETMPPWTDTTPQSMLDHTPFYTYA"
     gene            complement(6455..6763)
                     /locus_tag="A6A29_RS20420"
                     /old_locus_tag="A6A29_09000"
     CDS             complement(6455..6763)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046143.1"
                     /locus_tag="A6A29_RS20420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_09000"
                     /product="chaplin family protein"
                     /protein_id="WP_073721662.1"
                     /transl_table=11
                     /translation="MGAEIHGTGGGGGRVGKRAAVLGAVAGVALVPAGAAHANVIGIGN
                     AVFGNTCVSQGSAQAAGTTVAGSGLGSGNQAGLPLSLPRNHCGNSGIVCTALFGSSV"
     gene            7482..8246
                     /locus_tag="A6A29_RS20425"
                     /old_locus_tag="A6A29_09005"
     CDS             7482..8246
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6A29_RS20425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6A29_09005"
                     /product="hypothetical protein"
                     /protein_id="WP_073721664.1"
                     /transl_table=11
                     /translation="MTSFPFAAFMADQYRVHQPGDRRTEEWYRGLLRPAATRDTDGGLA
                     LAAERQGDLIGFRSALWLDGSGDHWSYGGSFGDLLLKRDGEESARSTYPYDAFKVPAED
                     SAYELTQNLSKIDTSDRNWLRSTALTTTWSFRSRLEPDVYSRGVPIFFPAYDLPVDGVN
                     TLPARGGIKVGLSAEGHAGYTPGTITEASLSYSYDGGTTWTRAPTEQRAGTWTATLDHT
                     GATGKQVMLRATLTDSNSNSVTQTITRAYDVR"
     gene            complement(8463..9584)
                     /locus_tag="A6A29_RS20430"
                     /old_locus_tag="A6A29_09010"
     CDS             complement(8463..9584)
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449463.1"
                     /locus_tag="A6A29_RS20430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_09010"
                     /product="PP2C family protein-serine/threonine phosphatase"
                     /protein_id="WP_073721666.1"
                     /transl_table=11
                     /translation="MPRNHPKRFTHPFEAIEPPRNVEVVIGVVSITVLVMVLGALSGSL
                     VWLLGLLVFLPGTASALCTVRQTQFVAVWTTLVVTITVVVRHGDGASWLDRILMVLLTL
                     TLGAASVYACHRRIHREHEMLRIRSTAAAMQRHILRPLPLLTDDVLVDGVYEPVQEDRL
                     VGGDIYDVVVSPWGTRVLIGDVQGKGLPAVGAAFAVIGAFREGAHREPTLTALADALDG
                     SVVRHNSYATQTGDDERFVTALILNIDAHDEVQALNCGHIPPQLLHDATVSTPTLDSGV
                     PLGLAELAAEPATVSWFTFPPGATLLLTTDGLTETRAADGTFYPVTERLAERASLSPTE
                     LPDALYDDARAFAGEGGQHDDVAVLSVRRSPHH"
     misc_feature    9544..9559
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.65"
                     /tool="antismash"
ORIGIN
        1 cgcgtgtccg ggacgtcgag cgtgcgccag gtccgcccgt tgtcatcggt gcgcaggatg
       61 gcgccccggc ggctgccgtc gcgaacgctg ttcacgacgt accaccagcg gtcgggattc
      121 ttcgcgtcca ccacgagcgg gcccgtgccg tccgcgactg gcagccgtcc caactggcgc
      181 caggaggcgc cgttgtcggt ggtcagccag ggagccgcct tctggtactg ggtgaggacg
      241 gcctgcttcg gccggctcga cgagagggcg acggaaccgg cctcgctgtt ggggccgacc
      301 ggctcccagc ggtcccttcg gctgtcgacc ggggtgactt cgaaggcgcc ggcgccggtg
      361 agccgctgtc cggtaccggc ggtgccgcgt accgagaccc ggtaggcacc ggccgtgctg
      421 ccggtcacct cggcccggta gtagccgttg gcgggatcga gggtggaagt gaccttgacg
      481 gccttgccgc gcggcggggt gacggtgacg actggcggtg cggccagccg ggtcggcggg
      541 gcgatgtgca cggtcgagtg accgtcgctc ggatcgggcg cggcctggac ggccagatac
      601 cggacggcga gcaggtaggg cacctcgatc gccggacccc ggtcgggagt cgccgtgaga
      661 cggccgctga tctccgtgtc ggccaccggc cgggcgacgc gcagcgagac cgtgacggtc
      721 gcggtccttc cggccggcac ggtgacccgc ttcggcgaga ccgtggccgc accgtcggtc
      781 cgcagcgagg tggcgaccgg gtgcttgccc gagttccgca gtgtcacctt cgcgatgccg
      841 ccgacgtgac gccgggacag atcggcgagc ccgaacgaca ccgacgccgg tgaggcgctg
      901 accgtggccg aggcggccgc ggccacatcg agccggcccg aaccctgcgt cgtgggcccg
      961 ctgcccttca gcggctcggc ggtgccgatc agcgcggcct tgatctcggc gggggcccgg
     1021 tcagggtgca gctggcgcag cagggccgcg gcgccggcga cgtgcggggc ggccatcgac
     1081 gtacccgaca tgcggtactg gccgggcccg tacagcgcct tggggatggt cgagcggatc
     1141 tccacgcccg gcgcgaccag atcgggcttc aggccgaagg ccggtgtggg gccgcgggac
     1201 gagaacgagg cgatctggtc ggtggtgtcg gtgccgcgca gtgtcaccga caccttcccg
     1261 gcggcgagcc gggtgctcaa ctccgcgtac tgggtcgagt cgatgcccat caccaccagg
     1321 cggtccatgc gcagtgagtc gccggaggcg tcgatccgcg cggacgagga cggcacctgg
     1381 accactcccg gctcggggtt cacctccggc ggccggacct ctcccggcgc tccggcgacg
     1441 aacaccgggc cgtcgttgga gggcaggccg gccagtacgg cgatcgcgcc gcgcttctcc
     1501 gcctcctgcg cccactccac cgcgctcccc gtcaggtact gggcgctggc ggcgacgagc
     1561 atctggcccc gtacgatctt tccgcgtacg tccccggccc gcttccagtc ctcggcggtg
     1621 ccctcgccga cgtcgacgag cggcgccgtg acggggcgct ccggtggatt cgcggacagg
     1681 atgccgcggt aactctggat caactcgggt tttctgcccg ccagataggc gctgggcagg
     1741 cgcagattgc tcgtcgaagc gccgaccgag acgacaccgt cggccgttcc cggactgctg
     1801 accgtacccg ggcccggacc gtcgttgccg gccgcggcca ccacgaccac accggcccga
     1861 acggccgcgg tcgcggcgca gcccagcgga tcggtgccgt cgcccgggcc gccgaggctc
     1921 aggttgatga catcggcgcg gtgcgggttg gccggatcgg aggcagcctc gattcccgcg
     1981 atgatgtccg atgtgtagcc ggagccatcg gcgtccatca ccttgtaggc cagcagattc
     2041 gcgccgggcg ccacgccggt gacgccgccc ttctcggccg ccttgcccgc gatgatgccg
     2101 gcgacatggg tgccgtggcc gttgtcgtcc atcgggtcct cgtcgccgtt gacgaagtcg
     2161 tgcccgccga cgaccttgtg ccccttgccg agtccgccgc ccaggtcggg gtggctgtag
     2221 tccacgccgc tgtccaggac cgcgacggtg gttcccttgc cggtgacgcg acttccggcg
     2281 gagtccttgc gctgccagac gccgggtgct ccgatcaagg gcacgctcgc gtccgtcttc
     2341 gtcagcaccc gggtgtcggg gcggacggcg gtgacgcccg gcagcgacga cagccgcccg
     2401 acctcggacg cgggcaccgt cattgccacg gcattgacga gcagccccag tctgcgggtg
     2461 gccgaagcgt gcagcccggc gctcttcacc gagccgagga acgtctcctg ccgcgcgtcg
     2521 agcgcgcggc gggcggtacc gacgccgcgg acggcctcgg cggacagcag cgatccaccg
     2581 ggtgcggcgg tgaccgcggg actcccggag agttcgacga gcactcgttg cgacgggtcg
     2641 ggcgcggacg cgtcggcggc acccggtatc gccgccagcg tgccggcgag aacaacggcg
     2701 gcgagggcgg tatgacgcga tcttcgtgac ttcattgcca tgcctaaagg tcttagcggt
     2761 gaggacactt gccgcacaac cgatcttcgt ggcccattgc tgccgctggc acaagtgggc
     2821 caccatccgt ccatgactcc cgaaacaccg caactcacgg cggcaggcat cgatccgttc
     2881 gacgagagcg tctaccgcgc cgtcctgtcc cggcggacgg cggcgcccgc cgaactcacc
     2941 gcggatctgt gctgttcggc cgagcgcgtc gccagggcgc tggaccggct ccgtgaccac
     3001 gggctggtcg gccgcctctc cggttcccgc cgccgttacg ccgcgatcga gccgggcgcc
     3061 gctgtggagg cgctggtgcg ggccagaagc aacgagctgg agcgggtccg ttcggcggcc
     3121 gacgagctgt cccggctgtt cgccgccgcg cgcaccggtg cgacgtacga ggacgaggtg
     3181 gagatcacca ccggcagcga ggcgctcggc cgctggttcg tccgccttca acaggaggcc
     3241 cacgaggacg tgatgaccct ggaccggcca ccgtacgccc tgaccacctc caacccggtg
     3301 gaggcgacgg cgctcggccg cggggtacgg taccgcgcgg tctacgctcc cgaagcactg
     3361 gaatggccgg gcgtcctcga cgacatccgc gagctggtcc ggcacggcga acaggcccgc
     3421 gtcctgcccg gcctgggcat caaactcgcc atcgccgacc gcaggctcgc cctgatgccg
     3481 ctctccctcg acctcaagaa tgtccgcgcc gcggtgatcc gcccctccac tctgctcgat
     3541 gccctcaccg actactggga aatgtgctgg gagcgggcac tgcccctcaa cgcccccgcc
     3601 gaggaccccc tcggcgagga ggaccgcctc gtcctcaccc tcctggtcag cggtctcaag
     3661 gacgaggcga tagcccgcca gcttggctgg tccgtccgca ccatgcgccg ccgcatcagc
     3721 cggctgcacg acctcctcgg cgccgccaac cgcttccagg ccggagtgat cgcggcccgc
     3781 cgcggctggt tgtgaggcag cacgccgaca gcagatgggg cgtcactgag tgggttccgg
     3841 ctctcgcgtc ggtgcggacc ggcctggagc ttccggtggg agtgaggcgg ggaatccggt
     3901 ggccgtcccc gggcccgagg gatcagcccg ctgtccccgc cgcgcggcgc aggcatccca
     3961 ggtggcgcat cgccggatgg ctccggtcct cgaccagtac ggtgcccggc tggaaggcgg
     4021 ggtggccgcg gcgaagcgtg caccgagcaa ctccccgtgg tacggccagt ggtactcggt
     4081 gcacgcggcc ggatggagac ccatgaagga ggggcggcgt cgttcgggtc gcgggacgcg
     4141 agcatgtaca cgatcaccct gatgggtgtc gtcttccctc gtcacaccga cgacgtacag
     4201 gcggtggcgg cggccgggga gtacggcata ccgctggctg cgtgtcatct cgcggtgggg
     4261 cccgatccgg aaacgggccc gggtactcga cgatctctcc gaaccgccgg tgctccacct
     4321 gcccggtgtg acggcccagc acggccagtc atacatcgga gcgttcactc ccgcgtgccc
     4381 ccgggggttc ctggatgagg agatcctctg aaaggtcgaa cacgtagtcc tacggatcct
     4441 ccacggtgcg gtccatcctg gctccttcgt tcgacgtcag gcgcgaacat cccgtccggc
     4501 gtgcaccgtg gagtgcgtgc gatccaccca cccagcggcc ccggctgctc actggacaag
     4561 ggcgcgtccc cagggtggcc gaactcaccg gagcacggcc gcttgggcgg gtgagggatg
     4621 cgcgagggga gaaggagctg tcggactgcg accaggccgg gccgccgatc ccggccaata
     4681 gggcgtaacg ggcaaaaggg tgacccgatt tcaggccatg ctcagatcat ggccctgacg
     4741 cgggcggccg ggtgagcccc ggaggatctt cacgtgcggg gctcattcgc agcggaatct
     4801 tcactttgtg atccgtggag cgtcggcatg gaggggaatc gatggcggcg gcacccgtat
     4861 gacctgcgaa gacatgtcca tatgacagcc aaccaaaatt ttgctgcttc tgtgaaccgt
     4921 cactccccat gtagtcatgg tcgcggcggc cccaaacaac ctgggggccg cctcattcat
     4981 gctcatgggg ggtattcacc atgtccaaac tcacccgtcg tcagaccctc ggggccgtca
     5041 ccggcgctgc cgcgggcata gcgctcgtcg gcgtggccac cgcgtccgcg gacaccgcgc
     5101 cggtcaaggg caacgacaag gcggccgcac ccggtggcac cggagggccc gcgtccttcg
     5161 acgaggtctt ccagggtcgc cgcatccagg gcctggccac cgaggctcat gcccacgccg
     5221 gacacagcac cggctaccgc gtcctggtcg acggccgcga actccatgtg atgcagcacg
     5281 gaaagagcgg ctggagcagc gcgatcaacc actacgagag attcgccaac ccgcgcgacg
     5341 ccgcgcgcac cgcggtcacc tcactcaagg gcgccgacgt cgttcctttc gaccccacag
     5401 cctgagccgg gagcaactgc catgaccgta cgcaagaacc aggcgaacct cacaggtgcc
     5461 gagaagaagg catttgtcga cgctgtactg gaagtcaagc gcaaaggtgt gtacgacgag
     5521 ttcgtcgccg cccaccgcct ccggttcgcg ctggacatga acaccgggat atacgtgggt
     5581 cacttcggcc cgtcgttcct gccctggcac cgcaagttcc tcatcgactt cgagaacgaa
     5641 ctccagaagg tcgacccgtc ggtgtcgatc ccttactggg actggaccgc ggacaacacc
     5701 gtcggctcgt cgctctgggc gccggacttc ctcggcggca ccggccgagc cctggacgac
     5761 caggtgatgg acggcccctt cgcctactcc tccggcaagt ggcccatcac catccaggtc
     5821 gacacccgcc cgttcctcgc ccgcaatctc gcctaccgcg tccccactct cccgacccgg
     5881 gccgaggtcg acgccgccct cgccattccc acctacgact ccgcgccctg gcgtgacggc
     5941 tcatccggct tccgctccac gctcgaaggc acggcgggat acatgagcat gcacaaccgg
     6001 gtgcacacct ggatctgggg tcagatggag acgagtgttt cccccaatga tcccgtgttc
     6061 tggctgcacc actcgttcgt cgacaagctc tgggccgact ggcacacact ccacccgagc
     6121 agcgccgagt acctccccgc cggaggcacc cccgacgtca tcgatctgga cgagaccatg
     6181 cctccctgga ccgacacgac cccacagagc atgctggacc acacgccctt ctacacctac
     6241 gcctgagttc agggcgtgac gggtctgctg ccggaatcga cggcgtccgc cgtcgattcc
     6301 ggcttgcagg ttctcgccgt gtgcgctgac cgtccatgac ggacacgaca ccgggacagc
     6361 ggccgtactt ccactgttgt gcgagccgcc ttcccggatc gtcaccctcg aagtgcacgc
     6421 cgagcgcgta cgaagtgggg gaggcccggc cggttcacac gctggagccg aacagagctg
     6481 tgcagacgat gccgctgttg ccgcaatggt tacgcggcag actcaacggc aagccggcct
     6541 ggttgccgct gcccaacccg ctcccggcca ccgtggtgcc cgcagcctgt gcactgccct
     6601 ggctgacgca cgtgttgccg aacaccgcgt tcccgatccc gatgacgttc gcgtgagcgg
     6661 cacccgccgg aaccaacgcc accccggcca ccgcacccag caccgcggcc cgcttaccca
     6721 cacgtccgcc accgccgccc gtcccgtgaa tctccgcacc catcgcgatt ctccttcgtc
     6781 cgggaccacg tcccggtgcc tgttcggacc acgcacggcc ggacggtccc gggccgcact
     6841 cccgaaccaa cgagagaacc cggctcccga cacgcggact ccgccagccc cttccgacgc
     6901 gggtcgccga ggcgaagccg agtcgaccgt gccccctcga tcgctctgga tctacggcgc
     6961 gggccacctc tccgacgcag actccacggc cggctacgcg atgtcgccgc acatcgacct
     7021 gtcctcgctg gaccgtcctg gaccacacgc tcagccaggg ccgcagccgc acgtgggacg
     7081 acacttggca gctgaacgga tcactcacgg ccggcagcaa cgtgcagaag ttctccgcct
     7141 ctgtcgaagg gcgcgcccag aagggcttct tcgagttccg gctcccacct ggcgcgccac
     7201 cgggtcgcgg agcggctcat cctatgtcta caacctctcg ttcccgaccg ggggccgctg
     7261 cggtccgacc gggtctaccg tccgagtgat tccaagctgg cccgggtcac cgagaagtgg
     7321 aacgccctcg ggaaggaggc cgactacctc gacgggctgt tcatcctccc cgcctggaac
     7381 aggaacacca ctgtcccggt gagcctgctc gccccggtcg aggtgccggg cacccgggac
     7441 cgcccactgc acgaccggcg acgacacctg gtcacggcgc gatgaccagc ttcccgtttg
     7501 ccgcgttcat ggccgaccaa taccgcgtcc atcagcccgg tgaccggcgt acggaggagt
     7561 ggtaccgcgg cctcctgcga ccggccgcga cgcgcgacac cgacggcggc ctggcgttgg
     7621 ccgccgagcg acagggcgat ctgatcggct tccggagtgc gctctggctc gacggctccg
     7681 gcgatcactg gtcctacggc gggtccttcg gcgacctgtt gctgaagcgc gacggcgagg
     7741 agagcgccag aagcacctat ccgtacgatg cgttcaaggt gccggccgag gactccgcgt
     7801 acgaactcac ccagaacctg tcaaagatcg acacctcgga ccggaactgg ctgcgttcca
     7861 ccgctctcac cactacctgg agcttccgct cccgcctgga gcctgacgtc tactcccgcg
     7921 gcgtgccgat cttcttcccg gcgtacgact tgccggtgga cggcgtgaac acgctgcccg
     7981 cgcggggagg tatcaaggtc ggtctgtccg ccgaaggcca cgccgggtac acaccgggca
     8041 cgatcacaga ggcatcgctg tcctactcct acgacggcgg caccacctgg acccgggcgc
     8101 cgaccgagca gcgggccggc acgtggacgg ccacccttga ccacaccggt gccaccggca
     8161 aacaggtcat gctgagagcc accctcactg actccaacag caactcggtt acccagacca
     8221 tcacccgcgc ctacgacgtt cgatagccac acggtcgggc cggggggcct tccgcaacag
     8281 ggacaccgcc cggccgcctg ttcgccgcct tacctgtgat cagcccgact tgtgcgggga
     8341 gctctgtgac gggcacccac gttgtggtgc ggttcgcaac gcgccgacgc cctggcgggt
     8401 cggttcggac cctgatcgct gcccgagggc acgtacttgt gtgtacgtgc gtcgtgtcgc
     8461 gctcagtgat gcggggaccg tcggacggac aggactgcga catcgtcatg ctgcccgcct
     8521 tctccggcga aggcgcgcgc gtcgtcgtag agggcatccg gcagttcggt gggagagagc
     8581 gaggcgcgct cggcgaggcg ttcggtgacg gggtagaacg taccgtcggc ggcacgggtc
     8641 tcggtgagcc catcggtggt cagtagcagc gtcgcgccgg gcgggaaggt gaaccagctg
     8701 acggtcgcgg gttcggcggc gagttcggcg agaccgaggg gcacaccgga gtcgagggtc
     8761 ggtgtgctga ctgtggcgtc atgcagcagc tgcggcggaa tgtgcccgca gttgagggct
     8821 tgtacctcgt cgtgcgcgtc gatgttgagg atgagcgctg tgacgaacct ttcgtcgtcg
     8881 ccggtctgtg tggcatagga gttgtgccgt acgacggagc catcaagggc atcggcgagc
     8941 gcggtcaggg tgggctcccg gtgagccccc tcacggaacg cgccgatgac ggcgaacgcg
     9001 gcgccgaccg caggcaggcc tttgccctgg acatcgccga tcagaacccg ggttccccac
     9061 ggggagacga cgacgtcgta gatgtcgccg ccgacgagcc ggtcctcctg cacaggctcg
     9121 tacacaccgt cgaccaggac gtcgtcggtg agcagaggca gagggcgcag gatgtggcgc
     9181 tgcatcgctg cggcggtgga gcgaatccgc agcatctcgt gttcccggtg gatacgccgg
     9241 tggcaggcgt acacggaggc cgctcccagg gtgagggtga gcagcaccat gaggatcctg
     9301 tcgagccacg acgccccgtc accgtgccgg accaccacgg tgatggtgac cacaagcgtg
     9361 gtccatacag cgacaaactg cgtctgccgt acggtgcaca gcgccgacgc cgttcctggc
     9421 agaaacacaa ggagccccag gagccacacc agtgacccgg acagggcacc gaggaccatc
     9481 accagaaccg tgatcgacac cacgccgatc acgacttcga cgttgcgcgg cggctcgatg
     9541 gcttcgaagg gatgtgtgaa tcttttcggg tgattgcggg gcacgggacc tcctgacagg
     9601 taggtggtgt attcccgtac gctaggcagg cccgtcgcgc gagagccgtt gccccgccgg
     9661 acaatgttcg gcgcggctcc ggccgtgggg tgaggaaggg cagcagccat ctgggacggg
     9721 cccgcgatac ggacgtactc cgatcaacac cgacggcttc gagacgacct ccggaacccc
     9781 tcgggggagg acaggttcac cagcgagtgg cggtccgcgg taggggctgg ttccggattc
     9841 tggtgtggat ccatcggcga ggacatccgc ggcctcgccg gcgcgagcgt gggcggttgc
     9901 ctgatgccgg aggggctcga aaggggtatt ccccatcgag tgtctggatc aggagctgga
     9961 tgtttttccc gactgccgac tgccgactgc cgactgccga ctgccgactg ccgactgccg
    10021 actgccgact gccgactgcc gactgcgcgc tgcgtggcgg gcttcggcgc ggggccggca
    10081 ggagctcggt ggtctctcgg gaacggcggc cctcctgggc agccgtcggc gcccgggtgg
    10141 cgtagcgtgg ccggtggaga gagtccgtcg cagtctcgtc gagccgcccg ccgtgagcgc
    10201 gatcggccgt gtgcccgcct cccctcgtgg cccgatggcg agaggaggag cgatgtccgt
    10261 cgtggcaccg gatgcggtga agcaggaggc gttcgcgggt cgcatgatcc aggtggtgaa
    10321 cgacacctgc ctgggtctga tggcgggcct gggccatgag agcggtctgt tcgacacgat
    10381 ggccggcatg gcgccggcca cgagt
//
