LOCUS       NZ_LWLE01000006        10399 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold14, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000006
VERSION     NZ_LWLE01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 225539)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225539)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 70238
            Orig. end                         :: 80637
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(196..1293)
                     /locus_tag="A6A29_RS13025"
                     /old_locus_tag="A6A29_31300"
     CDS             complement(196..1293)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="A6A29_RS13025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31300"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_079182726.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 7.3e-20, bitscore:
                     65.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEQRVLRVMGRVLVGPDEIRDELWAVGGRITYERPPGADDAV
                     TVTGWALPGLVDAHCHVGLDRHGPVDAATSEKQALTDREAGTLLIRDAGSPSDTRWIDD
                     REDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRDD
                     GDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEAALRDLVEAGIDCIEHATGLTEETIP
                     LFAERGVAIVPTLVNIATFPDLAAGGEAKFPRWSAHMRRLYERRYDTVRAAYDAGIPVY
                     VGTDAGGSLAHGLVAAEVAELVKAGIPPREALAATAWGARRWLGRPVLEEGAPADLVVY
                     DEDPRADVRVLAAPRHVVLNGRVIG"
     gene            1380..2486
                     /locus_tag="A6A29_RS13030"
                     /old_locus_tag="A6A29_31305"
     CDS             1380..2486
                     /NRPS_PKS="Domain: Aminotran_5 (74-338). E-value: 3.9e-27.
                     Score: 87.0. Matches aSDomain:
                     nrpspksdomains_A6A29_RS13030_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518886.1"
                     /locus_tag="A6A29_RS13030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31305"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_073720348.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.2e-22, bitscore:
                     71.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLTAAHFAAIERRVAGLLATQQDIVIMQGEALLPLE
                     GCIRGGARPGSTALNIVTGPYGQTFGNWLRDCGARVIDLSVPFHAAVTADQVAQALAAH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDVWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRKALPHAP
                     AQLEMLALEACAERIEAEGLDALMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRAPSGVDASSLVAQALAADPSLPLIAGGGALSKEMIRVNHYGVDATRGAVRSSLAA
                     LGAALADTGRQVDFEAARKAVSETWPSE"
     aSDomain        1602..2393
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A29_RS13030_Aminotran_5.1"
                     /evalue="3.90E-27"
                     /label="A6A29_RS13030_Aminotran_5.1"
                     /locus_tag="A6A29_RS13030"
                     /protein_end="338"
                     /protein_start="74"
                     /score="87.0"
                     /tool="antismash"
                     /translation="RDCGARVIDLSVPFHAAVTADQVAQALAAHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDVWGVDLCVIGAQKAMGGPAGVSA
                     VSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRKALPHAPAQLEMLALEACAERI
                     EAEGLDALMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVATTLRAPSGVDASSLV
                     AQALAADPSLPLIAGGGALSKEMIRVNHYGVDATRGAVRSSL"
     misc_feature    2656..2673
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    complement(2658..2673)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2668..2683
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2693..2707)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.08"
                     /tool="antismash"
     misc_feature    complement(2814..2828)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     gene            2839..3369
                     /gene="ectA"
                     /locus_tag="A6A29_RS13035"
                     /old_locus_tag="A6A29_31310"
     CDS             2839..3369
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607778.1"
                     /locus_tag="A6A29_RS13035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31310"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_198953059.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLRIDTPRVEDGAALWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARDENGEPIAFVTGYIRPDRPETLVVWQVAVDHGHRGKGLAATL
                     LDALTTRVAADQGLALVETTITPDNTASDRLFTSYAQRHDVSLEREVLFDSGLFPEETH
                     LPEVLYRIGPFHA"
     gene            3656..4918
                     /gene="ectB"
                     /locus_tag="A6A29_RS13040"
                     /old_locus_tag="A6A29_31315"
     CDS             3656..4918
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 2e-72.
                     Score: 236.0. Matches aSDomain:
                     nrpspksdomains_A6A29_RS13040_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="A6A29_RS13040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31315"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073720225.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.4e-79, bitscore:
                     259.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGMPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTQTRGEQVVQSLL
                     AICAEEPTAQFRGRGLVWGMEFADPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        3740..4744
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A29_RS13040_Aminotran_3.1"
                     /evalue="2.00E-72"
                     /label="A6A29_RS13040_Aminotran_3.1"
                     /locus_tag="A6A29_RS13040"
                     /protein_end="363"
                     /protein_start="28"
                     /score="236.0"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGMPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTQTRGEQVVQSLLAICAEEPTAQFRGRGLVWGMEFA"
     gene            5001..5399
                     /locus_tag="A6A29_RS13045"
                     /old_locus_tag="A6A29_31320"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="A6A29_RS13045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31320"
                     /product="ectoine synthase"
                     /protein_id="WP_073720226.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.3e-54, bitscore:
                     176.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWISPGTMYLLDGHEHHTLRPRTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            5403..6296
                     /gene="thpD"
                     /locus_tag="A6A29_RS13050"
                     /old_locus_tag="A6A29_31325"
     CDS             5403..6296
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="A6A29_RS13050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31325"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073720227.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-38, bitscore: 127.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTDVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAVKDLQGF
                     ERDGFLTVDQLITPDEVAVCHAELTRLIADPAVRADERSIIEPKSQDVRSVFEVHRLSE
                     VFARLVSDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RAVSVSIALTENFDTNGGLMIMPGSHKSFLGCAGETPEDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGRAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENAAEEPFAAP
                     VRRPEFIGARDFTPVK"
     gene            6404..7435
                     /locus_tag="A6A29_RS13055"
                     /old_locus_tag="A6A29_31330"
     CDS             6404..7435
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553309.1"
                     /locus_tag="A6A29_RS13055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31330"
                     /product="VOC family protein"
                     /protein_id="WP_073720228.1"
                     /transl_table=11
                     /translation="MTSHVRHLTIDCADAYKLAGFWSEVLGSPLSDDDVPGDPEALVEA
                     PGAALLFITVPEPKSTKNRLHVDIQPHDRSRDEEVERLLALGATMVGDHRKPNGRGWAT
                     LADPEGNEFCVECSAAERAALTGTRLPVTADDVTTAVRLSVDALGTAPADGWQVPAGTL
                     EWDCWETVEHLSDDLFAYAVQLGPRTPPLNGEVPYRWAAERAGGPANAVFADRAAGAAG
                     LLQTLEASGALLAAMVRTTPPEVRSYHSHGVSDPEGFAAMGIVETLVHTHDIAEGLGIS
                     WAPPAALCDRVLARLFPEAPSDEDRWTALLWSTGRTELPGRPRRTSWKWHGAPLGSDPA
                     AGA"
     gene            complement(7494..8564)
                     /locus_tag="A6A29_RS13060"
                     /old_locus_tag="A6A29_31335"
     CDS             complement(7494..8564)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="A6A29_RS13060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31335"
                     /product="alkene reductase"
                     /protein_id="WP_073720229.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLSGTQLANRIVMAPMTRSRAGAGGTATADTAEYYAQ
                     RASAGLIITEGIQPSVVGQGYSDTPGLHSAEQIASWRKVTDAVHARGGRIFAQLMHTGR
                     IGHPVLLPDGLVPVGASPVRAEGNAFTHEGPKEFVEPRELTRDEVRATVADFATAARNA
                     VEAGFDGVEVHGANGYLIHQFLAPNTNLREDEWGGSDEARIRFAVAVVEAVAAEIGAER
                     TGLRISPGNPYNGIDEPAPEAAYTALAAAIDPLGLAYLHVLEAVEIRELTLALRKRYTG
                     TLIINVLSDGPTGPDHHTVVDDGIADLISYGLLFLANPDLPARLRAGGPFNVPDPGTLY
                     GGGAEGYTDYPALDTV"
     gene            8710..9264
                     /locus_tag="A6A29_RS13065"
                     /old_locus_tag="A6A29_31340"
     CDS             8710..9264
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019765415.1"
                     /locus_tag="A6A29_RS13065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_31340"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_073720230.1"
                     /transl_table=11
                     /translation="MKTAHAGPADTTATPGTGVAADAADTAPCTDALPSAARGGPLSHA
                     VSRIARLHRITAGKLLKGLDLYPGQEFLMMHLWDAGAVRQSELIKALDLDPSTVTKMLQ
                     RLEQTGHVRRRPDPADRRAVLVEATDDSCALRTSVEAAWTNLEEHTLAGLGPEERAELA
                     RLLARVEANLCAEAEGCPGLA"
ORIGIN
        1 aggggtttta gcgtgcgtat tcgaatcttt cgtcagatgc gtggcacgtg cgttcctggg
       61 cgtcacccgt ttcaccgagc gcgagcggta cgggtgttga ccgcgccggg gtaccgcaga
      121 agtgactcaa cgagccggag gctcctgcgg acactgcctg tcaacacagc accccggccg
      181 accgcacccg gccgctcagc cgatcacgcg cccgttcagc acgacgtggc ggggcgcggc
      241 cagcacccgt acatccgcgc gcggatcctc gtcgtacacg accagatccg ccggggcccc
      301 ctcctccaga accggccgcc ccagccagcg ccgggcgccc caggccgtcg cggcgagggc
      361 ctcgcggggc ggaatgccgg ccttcaccag ttcggcgacc tccgccgcca ccagaccgtg
      421 ggccagcgag ccgcccgcgt cggttccgac atagaccggg atcccggcgt cgtacgcggc
      481 acgcacggtg tcgtagcggc gctcgtacag ccggcgcata tgggcggacc agcgagggaa
      541 cttggcctcg ccgcccgccg cgagatcggg aaaggtggcg atgttgacca gcgtcggaac
      601 gatcgccaca ccgcgttcgg cgaagagtgg aatcgtctcc tcggtcaggc cggtcgcatg
      661 ctcgatgcag tcgatcccgg cctccaccag atcgcgcagc gccgcctcgg cgaagcagtg
      721 cgcggtgacg cgggcgccca gccggtgcgc ctcggcgatg gccgcctcga cctcgccgcg
      781 cggccagcag gcggtcagat caccgtcgtc ccggtcgatc cagtcgccca ccagcttcac
      841 ccagccgtcc ccgcgccggg cctccctggc gacataggcg accaggtcgc ccggctcgat
      901 ctcatgggcg tagttacgga tgtaccggcg ggtcctggcg atgtgccggc cggccctgat
      961 gatcttcggc agatcctcgc ggtcgtcgat ccaccgggtg tcggacggcg atccggcgtc
     1021 ccggatcagc agcgtgccgg cctcccggtc cgtgagcgcc tgcttctcac tcgtcgccgc
     1081 gtcgacgggg ccgtgccggt cgaggcccac atggcagtgc gcgtcgacca gaccgggcag
     1141 cgcccacccg gtgacggtca ccgcgtcgtc cgcgcccggt ggccgctcgt acgtgatccg
     1201 cccgcccacg gcccacagct cgtcccggat ctcgtccgga ccgacgagca cccgccccat
     1261 cacccgcagc acccgctgct cctgatcact catacgcggc actgtacgag gccctcggta
     1321 ccctcgacgg agcactgttc gtgcgcagaa ccgctgaccg aaccgaagag agcaccaccg
     1381 tgacgcaccc cttcctcgac ctcgccccgc tcaccgccgc gcatttcgcg gccatcgagc
     1441 ggcgggtggc cggtcttctc gccacgcagc aggacatcgt gatcatgcag ggagaggcgc
     1501 tgctgcccct ggagggctgc atccggggcg gcgcccggcc cggctcgacc gcgctgaaca
     1561 tcgtcacggg cccgtacggg cagaccttcg gcaactggct gcgggactgc ggtgcccggg
     1621 tcatcgacct ctcggtgccg ttccacgcgg cggtcaccgc cgaccaggtc gcgcaggcgc
     1681 tcgccgccca cccggagatc gacttcgtct cgctggtgca cgcggaagcg gccaccggca
     1741 acaccaaccc ggtcgcggag atcggtgagg tggtccgggc ccacggcgcg ctgttcatgc
     1801 tggacgccgt cgcctcggtg ggcgccgaac cgctgctgcc ggacgtgtgg ggcgtcgacc
     1861 tgtgcgtgat cggcgcacag aaggcgatgg gcggaccggc cggggtgtcg gcggtgtcgg
     1921 tgagcgcgcg ggcctgggag cggatcgccg ccaaccccgc ggctccgcgc cgctcctacc
     1981 tctccctcct ggactggaag gagcgctgga tcgacggtgg ccgcaaggcg ctgccgcacg
     2041 ccccggcgca gctggagatg ctggcgctgg aggcgtgtgc ggagcggatc gaggccgagg
     2101 gcctggatgc gctgatggcc cgccacgcct cggccgcggc ggcgacccgg gcgggcgcgg
     2161 tggcgctcgg cggcggtctg gagccgtacg tccacgaggc gcgggacgcg gctccggtcg
     2221 ccacgacgct gcgcgccccg tcgggggtcg acgcctcctc gctggtcgcg caggcgctcg
     2281 cggcggaccc ctcgctgccg ctcatcgcgg gcggcggggc gttgtccaag gagatgatcc
     2341 gggtcaatca ctacggggtg gatgcgacgc ggggtgcggt gcgctcctcg ctcgcggccc
     2401 tgggggcggc gctggccgat acgggccggc aggtcgactt cgaagctgcc cgcaaggccg
     2461 tttcggaaac ctggccgagc gaatagattc cccaacaccc ccgccctgca cgcatcaccc
     2521 ggcggaccag aattaatgaa tgcggggagc tgctttatcg aaagcagctc cccgcattct
     2581 tctgcccgct ttccgtcacc ctaaacgcta ggattccgtg aacatggatc ctcgtaattc
     2641 atccgcatct cctgagagtt acaaatctgt gaccgactcc acatcgcgac tgaaatgccg
     2701 gaaaaattca ggtcacatgg tgagaaatcg cagccgattc gcgcgagagc acgcgcccgc
     2761 gtgatagcac aacagcaccg ttgacccaac ccctcgcctc gatgcgattt cgaaatttgc
     2821 tgggtaaatt caattcacat gaccgccgca ccagcagatt ttgcccgtgc ccgaagtgaa
     2881 ttcctcagaa tcgacacccc acgagtggag gacggagccg cgctctggcg cattgcccgc
     2941 gactccgagg tcctggacct caactcctcg tacagctacc tgctgtggtg ccgtgacttc
     3001 gccgcgacct ccgtggtcgc ccgcgacgag aacggcgagc ccatcgcctt cgtgacggga
     3061 tacatccggc cggaccggcc cgaaaccctc gtcgtctggc aggtggccgt cgaccacggc
     3121 caccgcggga agggcctggc cgccacgctg ctggacgcgc tgaccacgcg ggtcgccgca
     3181 gaccagggcc tggccctggt cgagacgacc atcaccccgg acaacaccgc gtccgaccgg
     3241 ctgttcacct cctacgcgca gcgccacgac gtgtccctgg aacgcgaggt gctcttcgac
     3301 agcggactgt tccccgagga aacgcacctg ccggaagtgc tgtaccgcat aggcccgttc
     3361 cacgcctgac gcgcgccgcg cccaccggac gcagcgcctg tcgcacgtcg cggccgccgg
     3421 acgcagcgcc cgacgcacgc cgcacccgac gcacacagcg ccacagcccg ccgccccgat
     3481 ccgcccagga accggagctg ccccaccgac ccgccgacgc ggcccgaccg acaccgcgtc
     3541 gacgccacga tcgtccgcgc cggaccgacc ccccggcgcg tcccggacca cgcttccacg
     3601 ccggaagcag gcttcacagc cacccgtcgc acccttctcg accaggagac ccgctgtgac
     3661 catcaccccg cccgccctga gtgtcttcga gaccctggag tcggaggtac ggagctactg
     3721 ccgcggctgg cccgcggtgt tcgaccgcgc gcagggcgcc cggctgaccg acgaggacgg
     3781 ccactcgtac ctcgacttct tcgccggtgc cggatcgctc aactacggcc acaacaaccc
     3841 ggtgctgaaa cgcgcgctga tcgactacat cgagcgcgac gggatcaccc acggcctgga
     3901 catggcgacc accgcgaagc gcgcgttcct ggagaccttc cagaacgtga tcctgcggcc
     3961 gcgcgacctc ccgtacaagg tgatgttccc cggcccgacc ggcaccaacg ccgtcgagtc
     4021 ggcgctcaag ctggcccgca aggtcaaggg ccgcgagtcc gtcgtctcgt tcaccaacgc
     4081 cttccacggc atgtcgctcg gctcgctcgc cgtgaccggc aacgccttca agcgggccgg
     4141 cgcgggcatc ccgctggtgc acggcacgcc gatgccgttc gacaactact tcgacggcac
     4201 ggtccccgac ttcctctggt tcgagcggct gctggaggac cagggctccg ggctcaacaa
     4261 gcccgccgcc gtgatcgtgg agaccgtcca gggcgagggg ggcatcaatg tcgcccgcgc
     4321 cgagtggctg cgcgcgctcc aggagctgtg ccaccgccag gacatgctgc tgatcgtcga
     4381 cgacatccag atgggctgcg gccgtaccgg tggcttcttc tccttcgagg aggccggcat
     4441 cgtgccggac atcgtcaccc tgtcgaagtc catcagcggc tacggcatgc ccatgtcgct
     4501 ctgcctgttc aagccggagc tggacatctg ggagccgggc gagcacaacg gcaccttccg
     4561 cggcaacaac ccggcgttcg tcaccgccgc cgccgcgctc gacgcctact gggccgacgg
     4621 ccagatggag aagcagacgc agacccgcgg cgagcaggtg gtgcagtcgc tgctggccat
     4681 ctgcgcggag gagccgaccg cgcagttccg cggccgcggc ctggtctggg gcatggagtt
     4741 cgccgacccg gcgcgcgcct cggccgtctg cgcccgcgcc ttcgagctgg ggctcctgct
     4801 ggagacctcg ggcccgcaga gcgaggtcgt caagctgctg ccgccactga ccatcacccc
     4861 cgaagagctg gacgagggcc tgcgcacgct ggcccgctgc gtccgcgaga cggcctgacc
     4921 ccattacccc tggggcaggc ggtacgaaaa gtaactccca ccgcacagag aagttgtaga
     4981 gagaaaggca ccgactcacc gtgatcgtcc gatcgttcag tgacatcgag aacaccgacc
     5041 ggcatgtgaa ggccgcatcc ggcacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtgggctt ctcgctccac gaaaccgtgc tctacgcggg cacggagacc tcgatgtggt
     5161 acgcgaacca catcgaggcg gtgctctgca ccgagggcga ggccgagctc accaacgacg
     5221 agaccggcga gaagcactgg atctcgcccg gcacgatgta tctgctggac gggcacgagc
     5281 accacacgct gcggcccagg accgacttcc gctgtgtgtg cgtcttcaat cctcccgtca
     5341 ccggacggga ggaccacgac gagaacggtg tctacccact gttgaccgag gagggctgaa
     5401 ccatgaccac cgatgtacgc gccgacctgt acccctcgcg cggcgccgcc gagatgacca
     5461 ctccccgcca ggacccggtc atctggtccg cgcccggcgc accggggccg gtcgccgtga
     5521 aggacctcca ggggttcgaa cgcgacggct tcctcaccgt cgaccagctg atcacgccgg
     5581 acgaggtcgc cgtctgccac gccgaactga cccggctgat cgccgatccg gcggtacgcg
     5641 ccgacgagcg ctcgatcatc gagccgaagt cgcaggacgt acggtccgtc ttcgaggtcc
     5701 acaggctcag tgaggtcttc gcccggctgg tgagcgacga gcgggtggtg ggccgggccc
     5761 gccagatcct cggctcggac gtgtacgtcc atcagtcgcg gatcaacgtc aagccgggct
     5821 tcggggcttc gggcttctac tggcactcgg acttcgagac ctggcacgcc gaggacggtc
     5881 tgccgaacat gcgggccgtg tccgtctcga tcgccctgac cgagaacttc gacaccaacg
     5941 gcgggctgat gatcatgccc ggctcgcaca agtcgttcct cggctgtgcg ggcgagacgc
     6001 cggaggacaa ctacaagaag tccttgcaga tgcaggacgc cggcaccccg tccgacgagg
     6061 cgctgacgaa gatggccgac cggcacggca tcaagctctt cacggggcgg gccggttcgg
     6121 cgacctggtt cgactgcaac tgcatgcacg gctcgggcga caacatcacc ccgtacccgc
     6181 gcagcaatgt cttcatcgtg ttcaacagcg tggagaacgc cgccgaggag cccttcgcgg
     6241 ctccggtccg ccgcccggag ttcatcgggg cgcgggactt caccccggtg aagtagcgcc
     6301 gggagcgggg ccggtggggg ggcggggtgg tgtcccgccc cgcccccccc acactgccat
     6361 aaccggctgg accggggccc ggtcaacggc gaaggtatgg cccatgacct cacacgtacg
     6421 tcacctcacc atcgactgcg ccgacgcgta caagctggcc ggtttctggt ccgaagtgct
     6481 cggctcgccc ctctccgacg acgacgtccc cggtgatccc gaggcgctcg tcgaggcccc
     6541 cggtgccgcg ctgctgttca tcacggtgcc cgagccgaag agcaccaaga accgtctgca
     6601 cgtcgacatc cagccgcacg accgctcccg tgacgaggag gtcgagcggc tgctggcgct
     6661 gggcgccacg atggtcggcg atcaccgcaa gccgaacggc cggggctggg cgacgctcgc
     6721 ggacccggag ggcaacgagt tctgtgtgga gtgcagcgcc gccgagcgag cggcactgac
     6781 cggaacccgg ctgccggtca ccgcggacga tgtgaccacg gcggtacggc tgtcggtgga
     6841 cgcgctgggc acggccccgg cggacggctg gcaggtgccc gcagggaccc tggagtggga
     6901 ctgctgggag acggtggagc atctgagcga cgatctcttc gcgtacgcgg tccagttggg
     6961 cccgcgcaca ccgccgctaa acggcgaggt cccctaccgg tgggccgccg agcgcgcggg
     7021 cggcccggcg aacgcggtct tcgcggaccg cgcggccggt gctgccgggc tgctgcagac
     7081 gctggaggcg agcggcgccc tgctggccgc gatggtgcgg acgaccccgc ccgaggtccg
     7141 ctcgtaccac tcgcacggcg tctccgaccc ggaggggttc gccgcgatgg gcatcgtgga
     7201 gaccctggtc cacacccatg acatcgccga gggactgggc atctcctggg cgccgcccgc
     7261 cgccctgtgc gaccgggtgc tggcccggct gttccccgag gctccgtcgg acgaggaccg
     7321 gtggacggcc ctgctgtggt ccacgggccg caccgagctg ccgggccggc cgcgccgcac
     7381 ctcctggaag tggcacggtg cgccgctggg ctcggacccg gcggccggcg cctgacggtg
     7441 ggagatccgg cgccggaccg ggccggcccc ggttcggcgt gcgcggccgg tcctcagacc
     7501 gtgtccagcg ccgggtagtc ggtgtacccc tcggccccgc cgccgtagag cgtgccgggg
     7561 tccgggacgt tgaacgggcc gcccgcgcgc agcctggccg gcaggtccgg gttggcgagg
     7621 aagagcagtc cgtaggagat gaggtccgcg atcccgtcgt cgaccaccgt gtggtggtcg
     7681 ggcccggtcg gcccgtccga caggacgttg atgatcagcg taccggtgta ccgcttgcgc
     7741 agcgcgagcg tcagctcacg gatctccacc gcttccagga cgtgcagata ggcgagtccc
     7801 agcgggtcga tcgccgcggc cagggcggtg taggcggctt ccggggcggg ctcgtcgatg
     7861 ccgttgtacg ggtttccggg tgagatgcgc agcccggtgc gctccgcgcc gatctccgcg
     7921 gcgaccgcct ccacgacggc gacggcgaag cggatgcggg cctcgtccga gccgccccac
     7981 tcgtcctcgc gcagattggt gttgggcgcc aggaactggt ggatgagata tccgttggca
     8041 ccgtgcacct ccaccccgtc gaagcccgcc tcgacggcgt tgcgggcggc ggtggcgaag
     8101 tccgcgacgg tcgcgcggac ctcgtcgcgg gtcagttcgc gcggctccac gaactccttc
     8161 ggcccctcgt gggtgaaggc gttgccctcc gccctgaccg gggaggcacc caccggcacc
     8221 agcccgtccg gcagcaggac cgggtggccg atccgtccgg tgtgcatgag ctgggcgaag
     8281 atccggccgc cccgggcgtg cacggcgtcg gtgaccttgc gccaggaggc gatctgctcg
     8341 gcgctgtgca gaccgggggt gtcggagtag ccctggccca ccaccgacgg ctggatgccc
     8401 tcggtgatga tcagcccggc cgaggcgcgc tgggcgtagt actcggcggt gtcggcggtg
     8461 gccgtgccgc ccgcgccggc ccggctgcgg gtcatcggtg ccatgacgat gcggttggcg
     8521 agctgcgtac cggagaggtc gatcgggtcg aacgcggtgg tcatgggagc tcccggagaa
     8581 tatatgtggt cggccaagca ttcgcgccgt tcgtcactgt agcgcattac ttggccgacc
     8641 aaggtaaagt tgggagacgc tgccggggca tcatcggact cgccgtagcc ccgccccgag
     8701 gagcccctga tgaagaccgc ccacgccggc ccggccgaca ccaccgcgac ccccggaacc
     8761 ggcgttgctg ccgatgccgc cgacaccgcc ccctgtacgg acgcgctgcc gagcgcggcg
     8821 cgcggcggcc ccctgagcca tgccgtctca cggatcgccc ggctgcaccg gatcacggcg
     8881 ggcaagctgc tcaaggggct ggacctctac cccggtcagg aattcctgat gatgcacctg
     8941 tgggacgcgg gtgcggtccg gcagtcggag ctgatcaagg cgctggacct cgacccctcc
     9001 acggtcacca agatgctcca gcgcctcgaa cagaccgggc atgtgcgccg ccgccccgac
     9061 ccggccgacc ggcgggcggt cctcgtcgag gccacggacg acagctgcgc cctgcgcacc
     9121 tcggtggagg cggcgtggac gaatctggag gagcacaccc tggcgggcct cggcccggag
     9181 gagcgtgcgg agctggcccg gctgctggcc agggtcgagg cgaatctctg cgcggaggcc
     9241 gagggctgcc ccggactcgc ctgagcccga agccccgtac ccgtacccgt actcacgctc
     9301 gcgccgcgcc cacccgagcc cgcccgagcc gccacgcact tcgccggccc ggaccaggac
     9361 gcccccgctc cgtcggcggg aaccacgacg tccccgttcc gccggctcgg gccaggaccg
     9421 tccgtactcc cctcctctcc cccgctcagg acagggcgtc cagcgcccgg tccacgtccg
     9481 cctcggtgtt gtagaggtgg aaggccgccc gcagattgcc cgcccgagcc gagaccatga
     9541 ctccggccct ggccagatcg ggttcacggg cgccgagccc gggcacggcg acgatggccg
     9601 attcaccggg aaccgccttg tggccgatcc ccgccaggcc ctcgcggaac cgggccgcga
     9661 gcccggtggc gtgggcgtac agggcatcgg tgccgacctc agccagcagg gccagggagt
     9721 gctcggcccc gtggtacgcg aggaaggcgg gcggttcgtc gaatcggcgg gcggaggggg
     9781 cgagccgctc gatcgggccg taggtgctgt tccaggggtc ctcggcggcc acccagccga
     9841 cgaacacggg cggcagggac gcctgcgcct cctcggtcac ggtgaggaac gacgcgcccc
     9901 gcgggcagag caggaacttg aaaccgccgg tgacggtgta gtcgtacgcc ccggcgtcca
     9961 acgggaacca gcccgccgac tgggtggcgt ccaccagtgt ccgggcaccg tacgaggcgg
    10021 cggccgcccg gacggcggcc aggtcggcga ccctgccgtc ggcggactgt acggaggaga
    10081 aggcgacgag cgcggtctcc ggtcgcaccg cgtcggcgag gtccgcgagc gggacgtacc
    10141 gcatccgcag gtcgccgcgg agcgcgaacg ggctgaccac cgagctgaac tcgccctcgg
    10201 ggacgagcac ttcggcgccc gggggcagcg aggctgcgat cagcccgaca tggacggtga
    10261 ccgagccgcc ggtggcgacc cggtcggggc cgacaccggc cagcccggcg aatgcgctcc
    10321 gggcggcctc caccgcttcg aagctgcccg cgcccgcccc tcggccggac gcgttgtggt
    10381 ccgcgagcgc cttgacggc
//
