LOCUS       NZ_LWLE01000003        10984 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold11, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000003
VERSION     NZ_LWLE01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 386182)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386182)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 64537
            Orig. end                         :: 75521
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(122..137)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.85"
                     /tool="antismash"
     gene            681..1343
                     /locus_tag="A6A29_RS08680"
                     /old_locus_tag="A6A29_27110"
     CDS             681..1343
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766165.1"
                     /locus_tag="A6A29_RS08680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27110"
                     /product="response regulator transcription factor"
                     /protein_id="WP_073719425.1"
                     /transl_table=11
                     /translation="MKDIRVLIVDDQMMVREGFSVLLNAMPGIAVAGEAVDGRQAVSQV
                     AALRPDVVLMDIRMPVMNGIEATREIVAQDADAKVLVLTTFDLDEYVYQALRAGASGFL
                     LKDASARQLADGVRVVASGEALLAPTVTRRLITEFAKVAETPRPPALARIGDLTERETE
                     VLVLIAQGLSNAEIASHLVVAESTIKTHVSRILVKLGLRDRTQAAVFAYEARLVTPG"
     gene            1586..2791
                     /locus_tag="A6A29_RS08685"
                     /old_locus_tag="A6A29_27115"
     CDS             1586..2791
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379872.1"
                     /locus_tag="A6A29_RS08685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27115"
                     /product="cytochrome P450"
                     /protein_id="WP_073719426.1"
                     /sec_met_domain="p450 (E-value: 2.7e-22, bitscore: 73.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHVSFDPWSPAFVADPYPAYAALRATGRAHYFEPTRQWLIPHYSD
                     VSGLLRDRRLGRTYLHRFTHEEFGRPAPPDAHEPFHTLNGQGILDLEAPDHTRIRRLVS
                     KAFTPRTVEGLAPTVRRLAAELVDALVAEGGGDLLAAVAEPLPVAVIAEMLGVPESDRA
                     LLRPWSAAICGMYELNPSEETARAAVRASVDFSAYLRELIAERRGKPGDDLISALIAAH
                     DEGERLSEQEMISTCVLLLNAGHEATVNTTVNGWRTLFAHPDRLAALRADHGLLPTAVE
                     ELMRYDTPLQMFERWVLDDIEIGGTVIPRGSEVALLFGSANRDPERFAEPDVLDLSRQD
                     NPHVTFGAGIHYCLGAPLARIELAASFGELLRKAPTMRVTAEPEWHPGYVIRGLKELRV
                     EL"
     gene            complement(2835..3164)
                     /locus_tag="A6A29_RS08690"
                     /old_locus_tag="A6A29_27120"
     CDS             complement(2835..3164)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018961801.1"
                     /locus_tag="A6A29_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27120"
                     /product="hypothetical protein"
                     /protein_id="WP_073719427.1"
                     /transl_table=11
                     /translation="MDTRREAVTGIAGCLVAAVGAVAGLAAWTPYGRDGLFGGFESASN
                     WTVVWLGLPIMTLGGTAAALGVFALVRGRWRPALGLMAAVTALAVLGYGFDVLAGPEPR
                     MCDPC"
     gene            complement(3326..4261)
                     /locus_tag="A6A29_RS08695"
                     /old_locus_tag="A6A29_27125"
     CDS             complement(3326..4261)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="A6A29_RS08695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27125"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_073719428.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.8e-13, bitscore:
                     44.6, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGARIVVTGGTGFIGAHVAKELLARDGVTVRFAGRRPVHADAPG
                     VEWVRAELTDSGSLHGICEDADVLVHLASKVSGTEESCRAVNEAGTAALMAEAVRAGTP
                     RVVHLSTTAVYGRGPHTGQDIADLTPAPVSAASATRLAGEQHALRAGAVVLRAGLVLGT
                     GDLWVVTALAELLKRVPGRWAGGAASLSVVDASDLGRLITAVALAPAPPGPAVFHAAHP
                     RPVRIGDLVAALAEHGIVPPPADEDWSWDRCLQEHADRPGIVSERQFHLLAQDHWYRSD
                     AVWQRTGVEPGPGPLDRLAASADWYRTLLT"
     gene            complement(4258..4887)
                     /locus_tag="A6A29_RS08700"
                     /old_locus_tag="A6A29_27130"
     CDS             complement(4258..4887)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742095.1"
                     /locus_tag="A6A29_RS08700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27130"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073719644.1"
                     /transl_table=11
                     /translation="MKSKPPQERAVRTRRALLRAAAECFDECGYYGAGTNKILARAGMT
                     QGAMYFHFKSKEELAHAVMLEQAADLELPAEPRGLQQLVNVTLMLAVEMQNNVLLRAGV
                     RLAVDPAGPARHDDSIYSWWAALFHKELVVARAAGELRDDVDEKSFSQTLVGAYTGTQL
                     MSEITTGRSDLTLRITNLWRFLLPGIATPEMLAALDLNGVPVMETG"
     misc_feature    4916..4936
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.78"
                     /tool="antismash"
     misc_feature    4932..4949
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            5001..5984
                     /locus_tag="A6A29_RS08705"
                     /old_locus_tag="A6A29_27135"
     CDS             5001..5984
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="A6A29_RS08705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27135"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_079182648.1"
                     /sec_met_domain="AfsA (E-value: 6.8e-18, bitscore: 58.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAHDAAGNCILDPVIDLVPQHYVHKSNAAEVYLKNWTRTGPDAF
                     RVGADWPATHAFYRVGTAFDPLLLCETIRQTFPLLCHAAYEVPIGHQLIWESFAFQVDS
                     AAYGADLGSEVELHVECFDLSYRGSRPAALSMRMVITRGGVEVATAHTRFTTHTPGVYR
                     RLRGAHADASAAMARSVPAPVPAAADDVRRSHVDDVVISPGTPGEDRQLRVLPPHPVHF
                     DHPVDHVPGMLLLESAHQLTYDLMRGSGRPVEITALDCTFTQYVELDAPCALRSEPLGP
                     DDAGRARLRVEAVQAGRVAFAATVTTTAATVAETGADGPAGKGREA"
     gene            5981..6949
                     /locus_tag="A6A29_RS08710"
                     /old_locus_tag="A6A29_27140"
     CDS             5981..6949
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="A6A29_RS08710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27140"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_073719430.1"
                     /transl_table=11
                     /translation="MTQQPEDTPRIPAAPVPTARITGVNLRHLHAFLAVADTLSFTHAA
                     ARMSSNQPALTRSIRRLEEHMGVRLFQRTTRQVSLTPAGAALRDELQVLLPRFEKALFP
                     EGAAPVLRLGFSWLLPDEWVHEAIRRFEEETGARVELRRRDDACAGVDQAAVDVAILRG
                     RPRRSGVKETLLGTERYVAAVPRRHPLAVREQLDWFELAEYPLVLNEVTGPVQQKDWGI
                     ARRPGTVLCCLNFDECIESVAAGKGISVVPDLVLRRNVHPSVAFVPLRGGPSIAISLIR
                     PVQGSHPLSDRFVRVVQQVLAPAARRGGTRESALAAPSAGD"
     gene            complement(7094..8323)
                     /gene="hemA"
                     /locus_tag="A6A29_RS08715"
                     /old_locus_tag="A6A29_27145"
     CDS             complement(7094..8323)
                     /EC_number="2.3.1.37"
                     /GO_function="GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (49-389). E-value:
                     2.8e-63. Score: 206.1. Matches aSDomain:
                     nrpspksdomains_A6A29_RS08715_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="hemA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /locus_tag="A6A29_RS08715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27145"
                     /product="5-aminolevulinate synthase"
                     /protein_id="WP_073719431.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.5e-62, bitscore:
                     206.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFHHDEFFLPPTDTLQKRGAYRTFLEIERRAGALPTTLKYGPGDG
                     RRISVWCGNDCLGMGQHPLVLAARKKAADEAGAGSGGSRDIAGENRHHVALEEELADLH
                     GMESALIFPSGYSANDAALTVLAGRPPGCVVFSDALNHASMTAGIRHSGAERHVFRHND
                     TDHLEELLAGTDPSAPKIIALESIYAMGSDPAPLERIAELAGRHGAFTYLDEVHAVGVY
                     GPRGAGLAAERGLGGHFDVVRGTLEMAFGTAGGYVAGSGAVIDAVRSFASAFLFTASLP
                     PAAAAGALAAVRHLKRSDGERAALHRKAALLQGLLRADGIPVASEAAHIVPVLVGDAVR
                     CRNVARRLLDQYGCYVQPVNAPSVPVGSERLRVTPAPHHSDDEIVGFAHALDSVWECEG
                     LPRTLRRVPV"
     aSDomain        complement(7157..8176)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A29_RS08715_Aminotran_1_2.1"
                     /evalue="2.80E-63"
                     /label="A6A29_RS08715_Aminotran_1_2.1"
                     /locus_tag="A6A29_RS08715"
                     /protein_end="389"
                     /protein_start="49"
                     /score="206.1"
                     /tool="antismash"
                     /translation="VWCGNDCLGMGQHPLVLAARKKAADEAGAGSGGSRDIAGENRHHV
                     ALEEELADLHGMESALIFPSGYSANDAALTVLAGRPPGCVVFSDALNHASMTAGIRHSG
                     AERHVFRHNDTDHLEELLAGTDPSAPKIIALESIYAMGSDPAPLERIAELAGRHGAFTY
                     LDEVHAVGVYGPRGAGLAAERGLGGHFDVVRGTLEMAFGTAGGYVAGSGAVIDAVRSFA
                     SAFLFTASLPPAAAAGALAAVRHLKRSDGERAALHRKAALLQGLLRADGIPVASEAAHI
                     VPVLVGDAVRCRNVARRLLDQYGCYVQPVNAPSVPVGSERLRVTPAPHHSDDEIVGFAH
                     "
     misc_feature    complement(8297..8299)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(8335..8349)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.09"
                     /tool="antismash"
     misc_feature    complement(8385..8398)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     gene            8532..9050
                     /locus_tag="A6A29_RS08720"
                     /old_locus_tag="A6A29_27150"
     CDS             8532..9050
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="A6A29_RS08720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_27150"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_073719432.1"
                     /transl_table=11
                     /translation="MSSQEKPQPRSADMGDVEELVRLRGYLLSSGSGAHVARTPEEDDL
                     WKRAYRAWLGRVLAGDEETVHVSVVGGAQALVACAVAVVDQRAPTVRCPSGRSGWVQTV
                     VVDPASRHRGLGAHVMDHALGWLRAAGAEAVVLQTTEAGSPLYRGLGFQPSGEELLSLS
                     LEASDSSRS"
     misc_feature    complement(9088..9099)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.88"
                     /tool="antismash"
     misc_feature    complement(9280..9294)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
ORIGIN
        1 ctgctggagg agcgcaccac catcgcccgt gagctgcacg acgtcgtcgc gcaccacatg
       61 tcggtcgtcg cgatccaggc ggaggccgcc ccctaccggg tggagaaccc gccgcccgag
      121 ctggagcagg cgttcgtcac catccgggag aacgcggtgg ccgccctcac cgagctgcgc
      181 cgggtcctcg gggtggtccg cgcggacgac tacgaggcgc cggacgcccc gcagcccacc
      241 ctcgacgggc tcgacgggct cctcgccaat gtgcgggagg cgggtctgga gtcggagaag
      301 acgatcaccg gtgcggtgcg cgagcttccg cagggcgtcg agctgtcggc gtaccggatc
      361 atccaggaag ccctcagcaa cacgctgcgg cacgcgccgg gcgccaccgc acaggtcgag
      421 ctcggctatg tgctcggcgg actggggctg cgcgtcgtca acgggccgcc caccggcctg
      481 gtgaagccct cgccgggggc cgggcacggg atcaccggaa tgcgggagcg ggtcgccatg
      541 ctgaacgggg agatgacggc gggggcgacc gaggacggcg gatacgagat cgccgccttc
      601 atccccgtac agaccacgta cgacacgcat gacacgcatg acacgcatga cagatacgac
      661 accgagcggg acggcaaggc atgaaagaca tccgggtcct gatcgtcgac gaccagatga
      721 tggtgcgcga gggcttctcc gtgctgctca acgcgatgcc ggggatcgcg gtcgccggtg
      781 aggcggtcga cgggcggcag gccgtctcgc aggtcgcggc cctgcgcccc gacgtggtgc
      841 tgatggacat ccggatgccg gtgatgaacg gcatcgaggc gacccgcgag atcgtggcgc
      901 aggacgcgga cgcgaaggtg ctggtactga cgacgttcga cctcgacgag tacgtgtacc
      961 aggcgctgcg ggccggggcc tccggcttcc tgctcaagga cgcctcggcg cggcaactcg
     1021 ccgatggtgt acgggtggtg gcgtccggcg aggcattgct cgcgccgacc gtgacccggc
     1081 ggctgatcac cgagttcgcc aaggtcgccg agacgccccg gccgcccgcc ctcgcccgga
     1141 tcggtgatct cacggaacgg gagacggagg tgctcgtgct catcgcgcag ggcctgtcga
     1201 acgcggagat cgcctcccat ctggtggtcg ccgaatccac catcaagacg catgtcagcc
     1261 gcatcctggt gaagctcggg ctgcgcgacc ggacccaggc ggcggtgttc gcgtacgagg
     1321 cccggctggt cacgcccggc tgagggccgg acgggcgggg cgccgggcag gggcccgtgg
     1381 cgccgggctc cgcgtgatgc cgggctccgc gtggcgccgg gccgcgcgtg atgccggcct
     1441 cgcgtgatgc cggcccccgt gtggcgccgg gcccccgtgt ggcgccggcc tcccgggacg
     1501 gcgtgaagcc tctgcgtacg gcgccggccg gccccgtacg caccggagcc cttgcggaag
     1561 cgcccggcgc cggtagcgtc gggtcatgca cgtgtccttc gacccctggt ccccggcgtt
     1621 cgtcgccgat ccgtaccccg cctacgccgc tctgcgtgcc accggccggg cgcactactt
     1681 cgagccgacc cggcagtggc tgatcccgca ctactccgat gtgtcgggcc tgctgcgtga
     1741 ccggcgcctg ggccgcacgt atctgcaccg cttcacccac gaggagttcg gccgcccggc
     1801 gccgcccgac gcgcacgagc cgttccacac cctcaacggg caggggatcc tcgatctgga
     1861 ggcccccgac cacacccgga tccggcggct cgtctccaag gcgttcaccc cgcgcacggt
     1921 cgaggggctg gcgccgaccg tgcggcggct ggcggccgaa ctggtggacg cgctcgtcgc
     1981 cgagggcggc ggcgatctgc tggccgcggt cgccgaaccc ctgccggtcg ccgtcatcgc
     2041 cgaaatgctc ggggtcccgg agtccgaccg ggcgctgctg cggccctggt cggcggccat
     2101 ctgcgggatg tacgagctga acccgtccga ggagaccgca cgggccgccg tccgcgcctc
     2161 ggtcgacttc tccgcgtatc tgcgcgaact gatcgccgag cgccgcggga agccggggga
     2221 cgacctgatc tccgcgctca tcgccgccca cgacgagggg gagcggctga gcgagcagga
     2281 gatgatctcc acctgtgtgc tgctgctgaa cgcggggcac gaggcgacgg tcaacaccac
     2341 cgtcaacggg tggcgcacgc tcttcgcgca cccggaccgg ctggcggcgc tgcgcgcgga
     2401 ccacgggctg ctgccgacgg ccgtggagga gctgatgcgg tacgacaccc cgttgcagat
     2461 gttcgagcgc tgggtgctgg acgacatcga gatcggcggc acggtcattc cgcgcgggtc
     2521 ggaggtggcg ctgctgttcg gctcggccaa ccgggacccg gaacggttcg cggagccgga
     2581 tgtgctcgat ctgtcccggc aggacaaccc gcacgtcacg ttcggggcgg gtatccacta
     2641 ctgtctgggc gcgccgctgg cccgtatcga actcgccgcc tcgttcggtg agttgctgcg
     2701 caaggcaccg acgatgcggg tgacggcgga gcccgagtgg catcccgggt atgtgatccg
     2761 ggggctgaag gagctgcgcg tggagctgtg agccgacgcg gcgggggcgg gagcgggggc
     2821 ggagtgcggc cggctcagca ggggtcgcac atccggggtt ccgggccggc caggacgtcg
     2881 aagccgtagc cgaggaccgc cagggccgtg acggctgcca tcaggcccag tgcggggcgc
     2941 caccggcccc ggacgagggc gaagacgccc agcgcggcgg cggttccgcc gagcgtcatg
     3001 atggggaggc cgagccagac cacggtccag ttggaggcgg attcgaagcc gccgaacagc
     3061 ccgtcccggc cgtacggggt ccaggccgcg agacccgcga cggcgccgac ggcggcgacc
     3121 aggcatccgg cgattccggt gacggcttct cgtcgagtgt ccatgtgcgg ggggacgcgg
     3181 cggggcggag tccggttccg gccgcgtcgg acgcacccgg agcgcgaacg cgcggcggtc
     3241 ccggacgcac ccggagtgcg aacttccggc ggttcgggtc gtcccggtcg gcccggagtg
     3301 cggtcgcccc ggagcgcggc gcgtttcagg tcagcagcgt gcggtaccag tcggccgagg
     3361 cggccagccg gtcgaggggg ccggggcccg gttccactcc ggtgcgctgc cagaccgcgt
     3421 cggaccggta ccagtggtcc tgcgcgagga gatggaactg cctctcgctc acgatccccg
     3481 gacggtcggc gtgctcctgg aggcaacggt cccaggacca gtcctcgtcg gcgggagggg
     3541 gcacgatccc gtgctcggcg agggccgcga ccaggtcgcc gatccgtacc ggccggggat
     3601 gcgccgcgtg gaacaccgcg ggccccggcg gtgcgggtgc cagggcgacg gcggtgatca
     3661 gccgccccag gtccgacgcg tccacgaccg acagcgaggc ggctccgccc gcccaccggc
     3721 cgggcacgcg cttcagcagc tcggccagcg cggtgacgac ccagaggtcg cccgttccca
     3781 ggaccagccc ggcgcggagc accaccgctc cggcccgcag cgcgtgctgt tcacccgcga
     3841 gccgcgtcgc gctggcggcc gacaccgggg cgggggtgag gtcggcgatg tcctggccgg
     3901 tgtgcgggcc ccggccgtag accgccgtgg tggacagatg cacgacgcgc ggtgtcccgg
     3961 cgcgtaccgc ctccgccatg agggccgcgg tgcccgcctc gttcacggcg cggcagctct
     4021 cctcggtccc ggagaccttg gacgccaggt ggaccagtac gtcggcgtcc tcgcagatcc
     4081 cgtgcagcga gccggagtcg gtgagttcgg cccgtaccca ctccacgccc ggtgcgtcgg
     4141 cgtggaccgg gcgccggccg gcgaagcgga ccgtcacccc gtcacgggcg agcagctcct
     4201 tggcgacatg cgcgccgatg aacccggtgc caccggtgac gacgatccgg gcgccggtca
     4261 ccccgtctcc atcacgggca cgccgttcag gtcgagtgcg gcgagcatct ccggtgtggc
     4321 gatgccgggc agcaggaagc gccacaggtt cgtgatgcgc agggtcaggt ccgagcggcc
     4381 cgtcgttatc tccgacatca gctgcgtccc ggtgtacgcg ccgacgagcg tctgcgaaaa
     4441 gctcttctcg tcgacgtcgt cccggagttc acccgctgcc ctggccacca ccagctcctt
     4501 gtggaagagc gccgcccacc aggagtagat cgagtcgtcg tgccgggccg gtcccgcggg
     4561 gtccacggcg agccgcactc cggcgcgcag cagaacgttg ttctgcatct ccacggccag
     4621 catgagggtg acgttcacca gttgttgcaa cccccgtggc tctgctggca gttccaggtc
     4681 ggccgcctgc tccagcatga ccgcgtgcgc cagttcctcc ttcgatttga agtggaagta
     4741 catggcgccc tgcgtcatgc ccgcgcgggc gaggattttg ttggtacccg ccccgtaata
     4801 tccgcattcg tcgaaacact cggccgcggc cctgaggagg gccctccggg tccggacggc
     4861 acgttcctgt ggtggtttgg acttcatcag gatgacccat gctttctcga caaacaaaga
     4921 gacctccctc tatgttaatg aaatgatgat cgccgctcaa ctcggcggcg agtgctctta
     4981 cacggagggg ggcggtcctc gtgcccgcac acgacgctgc cggaaactgc atcctcgacc
     5041 cagtcatcga cctagtgccg cagcactacg tgcacaagtc gaacgccgct gaggtctacc
     5101 tcaagaactg gaccaggaca gggcccgacg ccttccgcgt cggcgccgac tggcccgcca
     5161 cgcacgcctt ctaccgtgtg gggacggcgt tcgatcctct gctgctctgc gagacgatcc
     5221 gtcagacctt tccgctgctg tgccacgccg cgtacgaggt gccgatcggg catcagctca
     5281 tctgggagag cttcgccttc caggtcgaca gcgccgcgta cggtgcggac ctcggcagtg
     5341 aggtcgaact ccacgtcgag tgcttcgacc tgtcgtaccg gggcagccgt ccggccgccc
     5401 tttccatgcg catggtcatc acgcggggcg gcgtcgaagt ggcgaccgcc cacaccagat
     5461 tcaccacgca caccccgggg gtctaccggc gactgcgcgg ggcccacgcc gacgcgtcag
     5521 cggccatggc ccgttcggtg cccgcccccg tgccggccgc cgccgacgat gtccggcggt
     5581 cgcacgtgga cgatgtcgtg atcagccccg gcaccccggg cgaggacagg cagctcagag
     5641 tcctcccccc gcaccccgtc catttcgacc accccgtgga ccatgtgccg ggcatgctgc
     5701 tgctggagtc ggcgcaccaa ctgacgtacg acctgatgcg cggaagcggc cggccggtcg
     5761 agataaccgc cctggactgc acgttcaccc agtacgtcga gctggacgcg ccctgtgcgc
     5821 tccgctccga gcctctcggc cccgatgacg cgggccgtgc ccggctccgg gtcgaagccg
     5881 tgcaggcagg ccgtgtcgcc ttcgccgcca cggtcaccac gacggcggcg acggtggcgg
     5941 agaccggggc cgacggaccc gccgggaaag gcagagaggc atgacacaac aacccgagga
     6001 cactccccga atacccgccg cccccgtccc gaccgcacgc atcacggggg tcaacctccg
     6061 gcatctgcac gccttcctcg ccgttgccga caccctgagc ttcacccatg ccgccgccag
     6121 gatgtcgagc aaccagcccg ccctgacccg gtccatccgc cgtctcgaag agcacatggg
     6181 cgtacggctc ttccagcgca ccacccgcca ggtctccctg accccggcgg gcgcggccct
     6241 gcgcgacgaa ctccaagtgc tgctgccccg cttcgagaag gccctgttcc ccgagggcgc
     6301 cgcgcccgtt ctgcggctcg ggttctcctg gctgctcccc gacgagtggg tccacgaggc
     6361 catccggcgc ttcgaggagg agaccggcgc ccgcgtcgaa ctgcggcgcc gcgacgacgc
     6421 gtgcgccggg gtcgaccagg cggccgtcga cgtggcgatc ctgcggggcc gaccgcggcg
     6481 cagcggggtg aaggagacgc tgctcggcac cgagcggtac gtcgccgccg tcccgcgccg
     6541 gcatccgctg gccgtacggg agcagctgga ctggttcgaa ctggccgagt accccctcgt
     6601 actcaacgag gtcaccggcc ctgtccagca gaaggactgg ggcatcgccc gtcgccccgg
     6661 caccgtgctg tgctgcctca acttcgatga gtgcatcgag tcggtggccg cgggcaaggg
     6721 catcagcgtg gtgcccgacc tggtactgcg acgcaatgtc cacccctccg tggccttcgt
     6781 gccactgcgg ggagggccga gcatcgccat ctcgctgatc cggccggtcc agggcagtca
     6841 ccccctgtcg gatcggttcg tccgcgtggt ccagcaggtc ctggcaccgg ccgcgcggcg
     6901 cggcggcacc cgcgagtccg ccctcgccgc cccgtcggcg ggggactgac ggcgggccct
     6961 gacggcgggt cctggcggcg cggtcggtgt ccgggcccgg cggcgggccc gacggccggg
     7021 ggccgatggt gcgcatgggg tgtccgtccg gtacgacggt caccccatgc gcccacggca
     7081 atcggcggcg gggtcacacg ggtacccggc gcagcgtgcg gggcagtccc tcgcactccc
     7141 agaccgagtc cagggcatgg gcgaagccca cgatctcgtc atcgctgtgg tgcggtgcgg
     7201 gcgtgacgcg cagccgctcc gaacccaccg gcacactggg cgcgttgacg ggttggacat
     7261 agcagccgta ctggtccagg agccggcggg cgacgttgcg gcacctgacg gcgtcgccga
     7321 cgagcaccgg cacgatgtgc gcggcctcgg acgccacggg gatgccgtcg gcgcgcagca
     7381 ggccctggag gagcgccgcc ttccggtgca gcgcggcccg ctccccgtcc gaacgcttga
     7441 gatggcggac ggcggcgagc gctcctgccg cggccgccgg gggcagcgag gcggtgaaga
     7501 ggaacgcgga cgcgaagctc cggaccgcgt cgatcaccgc accggacccg gcgacgtagc
     7561 cgcccgccgt gccgaacgcc atctccagcg ttccccggac gacgtcgaag tgtccgccca
     7621 gcccccgttc ggcggccagg cccgcaccgc gcggcccgta cacgcccacc gcgtgcacct
     7681 cgtcgagata ggtgaacgcg ccgtgccgtc cagcgagttc ggcgatgcgc tccagtggcg
     7741 cggggtcgct ccccatggcg tagatgctct ccagggcgat gatcttcggc gccgacggat
     7801 ccgtaccggc cagcagctct tccagatggt cggtgtcgtt gtgccggaag acatgcctct
     7861 cggccccgct gtgccggata ccggcggtca tcgaggcgtg gttgagggcg tccgagaaca
     7921 cgacgcatcc gggcggtcgc cccgcgagca ccgtcagcgc ggcgtcgttc gccgagtatc
     7981 cggaagggaa gatgagcgcg gactccatgc cgtggagatc cgccaactcc tcctccagcg
     8041 ctacatggtg acggttttcg cccgcgatgt cccgggaacc tccgcttccg gcgcccgcct
     8101 cgtcggcggc cttcttcctc gcggccagga cgagcgggtg ctgccccatc ccgaggcagt
     8161 cgttgccgca ccacacgctg atccgcctgc cgtcgccggg gccgtacttc agcgtggtgg
     8221 ggagggcgcc ggccctgcgc tcgatctcca ggaacgtgcg gtaggcgccc cgcttctgga
     8281 gtgtgtcggt cgggggtaag aagaattcat cgtggtgaaa cactggccct ccgatgaaca
     8341 cggctttccg gacgtgacgc caggaatgta taggcacctg tcgggcaagg gaaatgccga
     8401 atccgtgggg tcatgcgaaa gctgcataaa tcgaggtcgg agcggctccg gaaccactca
     8461 ttccccacgt gcatagatga tgtacaggtc ccatttctca tgctgcgggt atcgccctaa
     8521 agtccttcgg tatgagttcc caggagaaac cgcagccgcg gtcggccgac atgggcgacg
     8581 tagaagagct cgtgcgcctg cgcggctatc tgctcagctc cgggagcggc gcccatgtgg
     8641 cccgtacacc ggaggaggac gacctctgga agcgcgccta ccgggcctgg ctcggccgtg
     8701 tcctcgccgg ggacgaggag accgtgcatg tctcggtggt cggcggtgcg caggctctgg
     8761 tcgcctgcgc cgtcgccgtc gtggaccagc gtgccccgac cgtgcgatgt cccagcggcc
     8821 ggtccggctg ggtgcagacc gtggtggtcg atccggcgtc gcggcatcgc gggctcgggg
     8881 cacatgtgat ggaccacgcc ctcggttggc tccgggcggc gggcgccgaa gcggtcgtcc
     8941 tgcagaccac cgaggccggc tccccgctct accgcgggct cggattccag ccgtcaggcg
     9001 aagagctgct ttccctgagc ctggaggcgt ccgactcctc aaggtcatga tcgtcgaaag
     9061 cgggtatgcc ggaatcgggt atgccgggga atcgctttcg cgccccttca ccggcccccg
     9121 ggtgccgagg gaattcatgt atgaggggcg aggggcgcac gcgcaaggac gtggacattc
     9181 cctgattccc gctacgagat cgaactggat gtctgacctc gcccccgccc cgcccccgcc
     9241 ccgcccccgc ccccgccgct gtcccggctc ccgtcgccga ccgacaggga tgatccgcga
     9301 acgggggcag gggcgagggg acgtggagtg tccgggccgg gagggtccgg ggcgacccgg
     9361 gaacctccgg ggcgacccgg gaactccggc tcgacccggg aactctgagg cgggcggtga
     9421 ggccccggtc gggtggtgag atccgggtca ggtcgcgatg tcgcggcgtc gcagcgccgc
     9481 caggcccgcc gccaccacga ccgccgcgac cgcggtcagc agcagcaccg gggtccaggc
     9541 catctccccg cccggcagct tcggcaggtg gctgaacggt gagaggttca tcgcggtctg
     9601 cggcaggtcc accgccgggc cgatccagcc gatcgccagg ctgagcccgg cgatgcccca
     9661 cgaggcggat gccagcttcg gtgcggcccc gtggagcagg accgcgatgc tgccgagcag
     9721 ccagaccgcg gggagctgga tcagggtcgc gcccaggatc gccggaaggt gatggccgta
     9781 tccgagggcc agaccgaggc cgccgacggc catgatgagc gcggcgccgc cgaaggcgat
     9841 gaccaggtgc ccggcggccc agccgatgcg gccgaccccg cccgcgagga cgggttcggc
     9901 gcgctggccg gtctcctcgc cgtgcagccg gagcaccgag gcgacgatgt acagcgcggc
     9961 caccatcccc agcatcccga ccatcgccgc caggaacgcg tcggtcagcc cggactgacc
    10021 gcccatccgc tcgaagatct ccttcgtctt ctcgttgtcg ccgaccagct ccgccgctcc
    10081 accggtcagt ccgccgaaga tcacgccgac ggcggcgaag gagagcgtcc agccgatcag
    10141 cgaaccgcgc tggagccgca gcgccagccc ggccgctgtg gagagccgcc ccgtggctgg
    10201 gcccggacgg gtcgccagga agctcatgcc gacatcgcgc cgcccggcca gggtgtaggc
    10261 gagcacggcc tggaccccga tcgcggcggc caggatcagc aggacccacc agcgctcgac
    10321 ggcgtaggcc cggacacgct cggcccagcc gatgggggag agccaggtca gtgcggagga
    10381 gccgtcggcc gttgcggagt cgcccgccgc cttcaggacg tacgcggccc cgatcaccgc
    10441 cgccgtcagc cccttcgcca gccgggcgct ctcggtgaac tgggcgacga tgcccgccgt
    10501 acaggcgaag agcacacccg taccggccac cgacagggcc agcgccaccg ctcccgcgcc
    10561 gcccgcaccg gcgcccgcca gcgcggcaac gatcaccagg gccagcgcgg cgttggcgat
    10621 cacggcggcc aggagggcgg cggtcagcgg agcgcgccgg cccaccatcg cggaggagag
    10681 catctcctga cgcccggtct cctcctcctc gcgggtgtgc cggatcacga tgatcaggct
    10741 catcacggcg gccagcgcgg ctccgaagcc gaccatgcgc caggtcacga gcccgccgag
    10801 cgagtcgtcg aagatcggcc cgtacagcgc gcggatcgag ctgttgccgt tcatcgaggc
    10861 ggccagttcg gcgcgctggg cggccgtctc gtacagcgag gagtaggact tggccacgga
    10921 ggagaaggaa cctccgagca ccaggaccca gatcggcatc atcagccggt cccggcgcag
    10981 ggcg
//
