LOCUS       NZ_LWLE01000002        13153 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold10, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000002
VERSION     NZ_LWLE01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 400740)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400740)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 217601
            Orig. end                         :: 230754
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13153
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="3"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13153
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13153
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8153](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8153
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(314..324)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            complement(1661..4864)
                     /locus_tag="A6A29_RS07560"
                     /old_locus_tag="A6A29_26045"
     CDS             complement(1661..4864)
                     /GO_function="GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128529.1"
                     /locus_tag="A6A29_RS07560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26045"
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="WP_073719192.1"
                     /transl_table=11
                     /translation="MHDDRTLVEGRLERALHQFIRPAQYAARTPLSLSVWHAPGEPVPV
                     TEALDRTYEPFSAGTEWGRPWSTSWFRLEGSVPGEWAGRRVEVVVDPGFSGEGPGFQAE
                     GMLYDLAGVPLKGIHPRNRHLTVAPSAAGGEPVALLLEAASNPSVLEHGFAPNPLGDVL
                     TAGDRPLYRFASADLAVLDEEVWHLVLDIEVLSELMRELPADRSRRHEILRALERMLDA
                     LDLHDVAGTAAAGRAELAEALSRPASASAHRVSAAGHAHIDSAWLWPLRETVRKASRTF
                     ANVTALAQDYPELVFACSQAQQYAWVKEHQPLIWERIKQAVADGQWSPVGSMWVESDAN
                     MPGGEALARQLVHGKRFFEEELGIETEEIWLPDSFGYTAAFPQLARLAGVRWFLTQKLS
                     WNQHNKMPHHTFWWEGIDGTRVFTHFPPVDTYNAQFHASELAHAERNFADKGRATRSLV
                     PFGWGDGGGGPTREMLEKARRLRDLEGSPRVSIEKPSVFFAAAEEEYGAQAPVWSGELY
                     LELHRATYTTQAKTKQGNRRSEHLLREAELWATAAALRTPGYRYPYETLDRVWKTVLLH
                     QFHDILPGSSIAWVHREARDTYEEVRSELADLVAEAVTSLGAAEGLVALNSSPYERCQV
                     IELDAEAAGVLPSGAHVQDLGEGRAAVLSRTPGLGAGLLDGSAVPDHPVTAVTAVTAVT
                     SGSIDAGITTDTTITADTGITTDTTADDGIVLDNGLLRITVDRDGLVASVRDLVAGREV
                     LAPGLRANLLQLHPDHPNQWDAWDIDRHYRHSRTDLTGADSVSLIDEGPLRAAVRVVRS
                     FGKSTIVQELRLTAGSRQLDIETEVDWQESEKVLKAAFPLDVHADRSTAEIQFGHVHRA
                     THDNTGWDAARFEICAHRWLRVAEPGYGIALLNDSTYGHDVTRTPHESGLGTTVRLTLL
                     RAPHSPDPATDLGTHRFRYALAPGAEVTDAVREGLALNLPLRAAVAPAVPSLVDTEHPA
                     VTVESVKLAEDRSGDVIVRLYESAGGRAETRLRVGFPVVGARITDLLERPLHPADTDED
                     GLVLALRPFQILTVRLTPA"
     misc_feature    complement(4815..4830)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.83"
                     /tool="antismash"
     misc_feature    4990..5007
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.51"
                     /tool="antismash"
     gene            5001..6185
                     /locus_tag="A6A29_RS07565"
                     /old_locus_tag="A6A29_26050"
     CDS             5001..6185
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237882.1"
                     /locus_tag="A6A29_RS07565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26050"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_073719193.1"
                     /sec_met_domain="DAO (E-value: 8.2e-71, bitscore: 233.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTRQPLDVIIVGAGIVGSACAYYAARSGLSVAVVDRGPVAGGTT
                     GAGEGNLLVSDKVPGAELDLALLSAALWRELADSLPPEIEYESKGGLVVAAGEESLAQL
                     TGTARGQRAAGVDARPVTAGEMAELEPHLASGMAGGFHYPQDAQVQPALAAAHLIGAAR
                     AEGARLYTGEEVTAVLTGPGGAVRGVRTRRRELHAPAVVNAAGTWGGELAALAGVRLPV
                     LPRRGFVLVTEPLPQLIRHKVYAAEYVSDVSSGSAALQTSAVVEGTPSGPVLIGASRER
                     VGFDRSLSVPVISRLAAQAAALFPFLADVRVLRAYHGFRPYLPDHLPAIGHDSRAPGLY
                     HACGHEGAGIGLAPATGKLVVAAIRGEEPALDPSPFSPDRFGAPEPSPSRSEPS"
     misc_feature    5238..5240
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6182..6526
                     /locus_tag="A6A29_RS07570"
                     /old_locus_tag="A6A29_26055"
     CDS             6182..6526
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100593.1"
                     /locus_tag="A6A29_RS07570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26055"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_073719194.1"
                     /sec_met_domain="Fer2_4 (E-value: 3.6e-23, bitscore: 75.9,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRLRRRRPAARTPAELAGAVPDPEFTIVIDSRPIPALPGQTVAA
                     ALWGAGILSWRTSRVNGLPRGAFCGIGSCFECLATVNGRPNQRACLLPAAPGDEITTQE
                     GDGHGTGLTR"
     gene            6621..8153
                     /locus_tag="A6A29_RS07575"
                     /old_locus_tag="A6A29_26060"
     CDS             6621..8153
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938968.1"
                     /locus_tag="A6A29_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26060"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_073719355.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2e-22, bitscore:
                     74.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.6e-11, bitscore:
                     37.8, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAAVTAADHGIPVTVLDAAARPGGQFYRAPAPGLGATRPEALHH
                     GWDAFTALAARFDRHVRSGRISHLARHQVWAVERATERSGEQSVERDSGRDSGAGGERT
                     GGCWRLHAVTGTEEDIGTVVDAQNLLIATGSHERQLPLPGWTTPGVVSAAGAQAMLKSG
                     LVLPGRRIVVAGSGPLLQAVAVSLARSGAEVPLLVEAGGYEFYARAPTTLATNPAKLAE
                     GARNAAQLLRHKVRMRTHSAVTAVHGRHRVEGVTVSRLDRDWRPVPGTGRRHDCDAVAL
                     GHGLEPRTGLATSLGCATRERADGTHGVVVDAQLRTTVPGIWAAGETGGIGGVQLALVE
                     GELAALSVVRLTRAEATRHSDGGLPRRAVALHRSRTRMRAFAEVMGAAHRPRPGWRQWL
                     SGDTEVCRCEEVTADRILTSVSELGAGDARTVKLLTRAGMGWCQGQTCGFAVRELIGPA
                     ASGAGPGRQALACPVLVSVLAGHPPATGAAGDSDAAETAGGAGATGGSGGTG"
     misc_feature    complement(8222..8232)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    8223..8233
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            8252..9169
                     /locus_tag="A6A29_RS07580"
                     /old_locus_tag="A6A29_26065"
     CDS             8252..9169
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612031.1"
                     /locus_tag="A6A29_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26065"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_073719195.1"
                     /transl_table=11
                     /translation="MQTNDTRTSPRTRPWRGVMVATPLPLKEDRSVDFDAFAEHVGRLV
                     EAGCDGVVPNGSLGEYQTLTDDERDRLVRVAVAAAGDGARVMPGVSAYGSDESRRWTEQ
                     AADAGAGSVLLLPPNGYRCDDTAIRAHYAAVAEVGLPVVAYNNPYDTKVDLTPRLLARL
                     HQEGSIVAVKEFSGDVRRAYEIAEEAPGLDVLIGADDVLLELALAGAVGWIAGCPNMLP
                     ASCVTLYRAAVAGDLGTALPLYRQLHPLLRWDSKPEFVQAIKASMDLVGWHGGPSRPPR
                     FPLPEASAAAVRAATEKALAEGLN"
     gene            9234..10235
                     /locus_tag="A6A29_RS07585"
                     /old_locus_tag="A6A29_26070"
     CDS             9234..10235
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="A6A29_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26070"
                     /product="proline racemase family protein"
                     /protein_id="WP_073719196.1"
                     /transl_table=11
                     /translation="MRTRRIFHAVDSHTEGMPTRVITGGVGTIPGATMAERRRYFLEHQ
                     DAVRTLLMYEPRGHAAMSGAILQPPTRPDADFGVLYIEVSGALPMCGHGTIGVATVLVE
                     TGMVAVTEPVTTVRLDTPAGLVSVDVQVEDGAATAVTFTNVPAFSAGLDRKVDVPGHGI
                     VHYDLAFGGNFYAILPLADLGLPFDRARKDDILAAGLAVMDAINATDPPVHPADPDIRG
                     LKHVQLLAPGSDAAHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGQLPLNTDFVNES
                     FIGTSFRGRLVEETSVAGTPAVVPTVTGRAWITGTAQFFLDPTDPFPEGFLL"
     misc_feature    complement(10302..10312)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            10347..11081
                     /locus_tag="A6A29_RS07590"
                     /old_locus_tag="A6A29_26075"
     CDS             10347..11081
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100589.1"
                     /locus_tag="A6A29_RS07590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26075"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_073719197.1"
                     /transl_table=11
                     /translation="MTRLSSLNTISAQEHLRDRVAHALRAALIAGDLRPGTIYSAPALA
                     SEFGVSATPVREAMLDLVREGLVEAVRNKGFRITELTDQDLDDFTELRAMIEVPTVGRI
                     AGLGKSRQLEALRPLALEIVHAARRHDILAYLEADRRFHLELLGLAGNRRLVEEVSNLR
                     KRSRLFGLNRLAESGQLIASAEEHIELLDLIVAGDAKRAEACMLAHMSHVRSLWADGGE
                     VGEGEGVERITHRRVGRGTAGA"
     gene            complement(11151..11531)
                     /locus_tag="A6A29_RS07595"
                     /old_locus_tag="A6A29_26080"
     CDS             complement(11151..11531)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574976.1"
                     /locus_tag="A6A29_RS07595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_26080"
                     /product="VOC family protein"
                     /protein_id="WP_073719198.1"
                     /transl_table=11
                     /translation="MACRISELVLGCHDPEALARFWCEVLDFVVLDREDGEMVEIGPRE
                     GFGGPQPTIILSRRDEPEKGKSRLHIDVNATDRDQDAELERLLKLGARPADIGQTGEEQ
                     WHVLTDPEGNEFCLLKARLNPI"
     misc_feature    12638..12648
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(12779..12799)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 23.14"
                     /tool="antismash"
ORIGIN
        1 gccggtgcgg aagcgcagat caccggcagc ctggccgcct gcgcgggatc gtgcggcccc
       61 ggcaatctgc acctcatcaa cgggctctac gacgcccacc gctccatggc cccggtgctc
      121 gccctggcct cgcacatccc ctccagcgag atcgggctcg gctacttcca ggagacccat
      181 ccggagctgc tcttccagga gtgcagccac tacaacgaga tgatctccaa cccgcagcag
      241 atgccgcgcc ttctccagac ggccatccag cacgcgatcg gccgcggcgg ggtcagcgtc
      301 gtctcgatgc cgggtgacat tgcctcgcag cccgcccccg agaagtcgat cgaacacgcc
      361 ctggtcacct cacgcccgtc ggtacggccc ggggacgagg agatcgagaa gctctgccgg
      421 atggtcgacg aggccaaacg ggtgaccctg ttctgcggca gcggaaccgc tggcgcgcac
      481 gccgaggtga tggagttcgc ggagcgggtg aagtccccgg tcggccacgc actgcgtggc
      541 aaggagtgga tccagtacga caacccgtac gacgtcggca tgagcgggct gctcggctac
      601 ggtgccgcct acgaggcgac caacgagtgc gacctgctca tcctgctcgg caccgacttc
      661 ccgtacaacg ccttcctccc ggacgatgtg aagatcgtcc aggtcgatgt gcgtcccgaa
      721 cacctgggcc ggcgttccaa gctcgatctg gcggtctggg gagatgtgcg cgagacactg
      781 cgctgtctga cgccgagggt gaaggccaag accgaccgca agttcctcga ccggatgctg
      841 aagaagcacg ccgacgcgct ggagggcgtg gtcaaggcgt acacccgcaa ggtcgacaag
      901 catgtgccga tccacccgga gtacgtcgct tcggtactgg acgaactcgc ggacgacgat
      961 gcggtgttca ccgtcgatac cgggatgtgc aatgtatggg cggcccgcta tctctcgccc
     1021 aacggcaagc ggcgggtgat cggttcgttc agtcacggtt cgatggccaa cgcgctgccg
     1081 caggcgatcg gcgcccagtt cgtcgaccgg aaccggcagg tcgtgtcgat gtccggcgac
     1141 ggcggattca ccatgctcat gggcgacttc ctcaccctgg tccagtacga cctgccggtg
     1201 aagatcgtcc tcttcaacaa ctcctctctg ggcatggtgg agttggagat gctggtggcg
     1261 ggtctgccgt cgttcggtac gacgaacaag aatccggact tcgccgccgt cgcccgcgcc
     1321 gccggggcgt acggggtgcg ggtggagaag cccaaacagc ttgaaggcgc cctcaaggac
     1381 gccttcaagc acaagggccc cgcactggtc gacgtggtca ccgacccgaa cgccctgtcc
     1441 atcccgccga agatcagcgc ggacatggtg accggcttcg cgctctccgc cagcaagatc
     1501 gtgctggacg ggggagtggg ccggatgctc cagttggccc gctccaatct gcgcaacgtc
     1561 ccccgcccct gacctcgcgg tgaggggccg gggcgggggc gaggcaggag cgggagccgg
     1621 gcgacgcacg ggccgggcgc ggccgtgatg aggggcgcgc ctacgccggg gtcagccgca
     1681 ccgtcaggat ctggaacggc cgcagcgcca ggacgagacc gtcctcgtcc gtgtccgccg
     1741 ggtgcagtgg ccgctccagc agatcggtga tccgggcccc tacgacgggg aatccgaccc
     1801 tgagacgagt ctcggcccgg ccgccggccg actcgtagag ccgcacgatc acgtccccgc
     1861 tgcggtcctc ggccagcttc acggactcca cggtcaccgc cgggtgctcg gtgtccacga
     1921 gcgacggaac ggccggtgcc acggccgcgc gaagcggcag attgagcgcg aggccctccc
     1981 gtaccgcatc ggtgacctcg gcgccggggg ccagcgcgta ccggaaccgg tgcgtgccga
     2041 ggtctgttgc cgggtccgga ctgtgcggag cgcgcagcag tgtcagccgt acggtcgtgc
     2101 cgagcccgga ctcgtggggc gtcctcgtca cgtcgtggcc gtaggtcgag tcgttgagca
     2161 gcgcgatgcc gtaacccggc tcggcgaccc ggagccagcg gtgggcgcag atctcgaaac
     2221 gggccgcgtc ccagcccgtg ttgtcatgcg tcgcgcgatg gacatggccg aactggatct
     2281 cggccgtgga acggtcggcg tggacatcca acgggaaggc cgccttgagc accttctcgg
     2341 actcctgcca gtccacctcg gtctcgatgt cgagctgccg gcttcccgcg gtcagccgca
     2401 gctcctgaac gatcgtggac ttcccgaacg aacgcaccac gcggaccgcg gcccgcaacg
     2461 gcccctcatc gatcagggag acggagtcgg cgccggtcag atcggtacgg ctgtgccggt
     2521 agtgccggtc gatgtcccag gcgtcccact ggttggggtg gtccgggtgc agctggagga
     2581 ggttggcgcg caggccgggg gccaggacct cacgccccgc caccagatcg cgcaccgagg
     2641 cgaccagccc gtcccggtcg acggtgatcc gcagcagccc gttgtccagc acgatcccgt
     2701 cgtccgccgt cgtgtcggtc gtgattcccg tgtcggccgt gatcgtcgtg tcggtcgtga
     2761 ttcccgcgtc gatcgagccg ctcgtgactg ccgtgaccgc tgtgaccgcc gtgaccgggt
     2821 gatccgggac ggccgagccg tccagcagcc ccgcacccag ccccggcgtc cgggacagca
     2881 ccgccgcgcg cccttcgccc aggtcctgta catgggcacc ggacggcagc accccggccg
     2941 cctccgcgtc cagctcgatc acctgacacc gttcgtacgg agaggagttg agcgccacca
     3001 gaccctcggc cgcaccgagc gaggtcacgg cctcggccac caggtccgcc agctcggagc
     3061 ggacctcctc ataggtgtca cgggcctcgc ggtgcaccca ggcgatcgac gagccgggca
     3121 ggatgtcatg gaactgatgc agcagcacgg tcttccacac ccggtccagc gtctcgtacg
     3181 ggtagcggta gccgggcgtg cgcagggccg ccgccgtggc ccacaactcg gcctcgcgca
     3241 ggaggtgttc gctccgccgg ttgccctgct tggtcttggc ctgggtggtg tacgtggccc
     3301 ggtgcaactc caggtacagc tcgcccgacc agaccggcgc ctgtgccccg tactcctcct
     3361 cggccgccgc gaagaagacc gaaggcttct cgatcgacac ccggggtgag ccctccaggt
     3421 cccggagcct gcgagccttc tccagcatct cgcgggtcgg accgccgcct ccgtcgcccc
     3481 acccgaacgg caccagggag cgggtggcgc gccccttgtc ggcgaagttc cgctcggcgt
     3541 gcgcgagttc actcgcatgg aactgggcgt tgtaggtgtc gaccggtggg aagtgggtga
     3601 acacacgtgt gccgtcgatg ccctcccacc agaaggtgtg gtgcggcatc ttgttgtgct
     3661 ggttccacga cagcttctgc gtgaggaacc accgcacccc ggccagccgg gccagctgag
     3721 ggaaggccgc cgtgtagccg aaggagtccg gcagccagat ctcctccgtc tcgatgccca
     3781 gttcctcctc gaagaacctt tttccgtgca cgagttggcg ggccagtgcc tcgccgccgg
     3841 gcatgttggc gtcggactcc acccacatcg agccgaccgg cgaccactgg ccgtccgcca
     3901 cggcctgctt gatccgctcc cagatgagcg gctggtgctc cttcacccag gcgtactgct
     3961 gcgcctgcga acaggcgaag acgagctccg ggtagtcctg ggcgagtgcc gtgacattgg
     4021 cgaaggtgcg ggacgccttg cgtaccgtct cgcgcagcgg ccacagccac gccgaatcga
     4081 tgtgcgcgtg cccggcggcc gagacacggt gcgcgctcgc cgacgcgggc cgggacagcg
     4141 cctcggccag ttcggcacgg cccgccgccg ccgtacccgc cacatcgtgc agatcgaggg
     4201 cgtcgagcat ccgctccagc gcccgcagga tctcgtgtcg cctggagcgg tcggcgggca
     4261 gctcccgcat cagctcggac aggacctcga tgtccaggac gagatgccag acctcctcgt
     4321 cgagtacggc gaggtccgcc gaggcgaacc ggtacagggg ccggtccccg gccgtcagta
     4381 cgtcaccgag cggattcggt gcgaagccgt gctccagcac ggacggattg gacgccgcct
     4441 ccaggagcag cgccaccggt tcaccgcccg ccgccgacgg ggccaccgtg aggtggcgat
     4501 tgcgcgggtg gatgcccttg agagggacac ccgcaaggtc gtacagcatg ccctccgcct
     4561 ggaacccggg accctcgccg gagaacccgg ggtcgacaac cacctcgacc cggcgccccg
     4621 cccactcgcc gggcaccgac ccttcgagcc ggaaccagct ggtggaccag ggcctgcccc
     4681 actccgtacc ggcgctgaaa ggttcgtacg tccggtcgag ggcctcggtc acgggcacgg
     4741 gttcgcccgg ggcgtgccag acggacaggg agagcggggt acgggccgcg tactgggcgg
     4801 ggcggatgaa ctggtggagc gcgcgttcga ggcggccctc caccagcgtg cggtcgtcgt
     4861 gcatcggatg cctccggatc tgtgcgcgga acgtcacgcg ttcacatgcc cggtgaggac
     4921 ttccgatagc ccgcgcatcc cgaaatctcc ctgctgcacg ctctcttggc accctctgcg
     4981 caatgtgaca tattactgcc gtgttcacga gacagcccct ggacgtcatc atcgtcggcg
     5041 ccggcatagt cggttccgcc tgtgcttact acgcggcccg atccggtctt tccgtcgctg
     5101 tcgtcgaccg ggggccggtc gccggaggaa ccaccggagc cggtgaggga aacctcctgg
     5161 tctccgacaa ggtgcccggc gcggagctgg atctcgcact cctgtccgcc gcgctctggc
     5221 gagaactggc cgactcctta ccgccggaga tcgagtacga gagcaagggc ggtctggtgg
     5281 tggcggccgg tgaggaatcc ttggcgcagc tgaccggaac ggcgcgcggc cagcgagcgg
     5341 ccggcgtcga cgcgcgcccg gtcaccgcgg gggagatggc cgagctcgaa ccccacctgg
     5401 cctccgggat ggccggcgga ttccactacc cgcaggacgc acaggtccaa cccgccctgg
     5461 ccgcagctca tttgatcggc gctgctcgcg ccgaaggggc gcggctgtac accggtgagg
     5521 aggtgaccgc cgtactcacc ggaccgggcg gcgcggtacg cggggtacgg accagaaggc
     5581 gcgagctgca cgcccccgcg gtcgtcaacg ccgccgggac ctggggcggc gaactggccg
     5641 ccctggccgg ggtccggctg ccggtcctgc cacgacgcgg gttcgtcctg gtcaccgagc
     5701 cgctgccgca gctgatccgg cacaaggtgt acgcggcgga gtacgtgtcc gacgtgtcca
     5761 gcgggtcggc agcactccag acctccgccg tggtcgaggg aaccccctcc gggccggtac
     5821 tcatcggcgc cagcagggaa cgcgtgggtt tcgaccggtc gttgtccgtc ccggtgatca
     5881 gccggctggc cgctcaggcc gccgccctct tccccttcct ggccgatgtc cgggtactgc
     5941 gcgcctacca cggcttccgg ccctatctgc ccgaccacct gcccgccatc ggccacgact
     6001 cccgcgcacc cggcctgtac cacgcctgcg gccacgaggg cgcggggatc ggtctcgccc
     6061 ctgccaccgg aaagctggtc gtcgcggcga tccgtggcga ggaacccgcc ctcgacccga
     6121 gcccgttcag ccctgaccgg ttcggcgccc ctgagccatc tcccagtcga tcggagccct
     6181 cgtgaagcgc ctgcgccgcc gacgcccggc cgcccgcacc cccgccgaac tggccggtgc
     6241 ggtgcccgac ccggagttca cgatcgtcat cgactcccgc ccgattcccg cactgcccgg
     6301 ccagaccgtc gccgccgccc tgtggggggc gggcatcctg agctggcgga catcccgcgt
     6361 gaacggcctt ccgcgcggcg ccttctgcgg catcgggtcg tgcttcgaat gcctggccac
     6421 cgtcaacggc cgccccaatc agcgggcttg cctgctgccc gccgcgcccg gcgacgagat
     6481 caccacacag gaaggggacg gccatgggac gggactcacg aggtgaacag cgcatacccg
     6541 caccggaggt attcccggct ccgcccgtga cgtcccgccc ggaggcaggg ctcgcggtgg
     6601 tgggcgcggg gcccgccgga atggcagcgg cggtgaccgc ggccgatcac ggcatcccgg
     6661 tcaccgtgct ggacgcggcg gcccggccgg gcggacagtt ctaccgcgca ccggcgcccg
     6721 gcctcggcgc cacccggccg gaggccctgc accacggctg ggacgcgttc accgcgctcg
     6781 ccgccaggtt cgaccggcat gtgcgttccg gacggatcag ccacctcgca cggcaccagg
     6841 tgtgggcggt cgaacgggcg actgaacggt ccggtgaaca gtccgttgaa cgggactccg
     6901 gccgggactc cggagcgggc ggtgaacgga ccggtggatg ctggcggttg cacgcggtca
     6961 ccggtaccga agaggacatc gggacggtgg tcgacgcaca gaacctgctc atcgccaccg
     7021 gatcacacga gcggcagctg ccgttgccgg gctggaccac accgggcgtc gtgagcgccg
     7081 ccggggcgca ggccatgctc aaatccggcc tggtcctgcc gggccggcgc atcgtcgtcg
     7141 cgggcagcgg cccgctgctc caggcggtcg ccgtctccct ggccaggtcc ggggccgagg
     7201 tgccgctgct cgtcgaggcc ggagggtacg agttctacgc gcgagcgccc accacgctgg
     7261 ccaccaaccc ggccaagctg gcggaaggag cccgcaacgc ggcccagttg ctgcggcaca
     7321 aagtacgcat gcggacgcac agtgcggtga ccgccgtgca cggccgtcac cgggtggaag
     7381 gcgtcacggt cagccgtctg gaccgtgact ggcgtccggt gccgggcacc ggtcgccgcc
     7441 acgactgcga cgccgtcgcc ctcggccacg ggctggagcc acgcaccggg ctggccacgt
     7501 ccctgggctg cgccacccgc gagcgtgccg acggcacaca tggggtggtc gtggacgcgc
     7561 agttgcggac caccgtgccc ggcatctggg ccgcagggga gaccggtggt atcggaggtg
     7621 tccaactggc cctggtggaa ggtgaactgg ccgcgctgtc agtggtccgc ctgacccgtg
     7681 cggaggcgac gcgccacagc gacggcgggt tgccccggag ggcggtcgcg ctgcatcgct
     7741 cccggactcg gatgcgtgcc ttcgccgagg tcatgggtgc cgcgcaccgg ccgcgtcccg
     7801 gctggcgaca gtggctgtcc ggtgacaccg aggtctgccg ctgcgaagag gtcaccgccg
     7861 accggatcct gacctcggtc tcggaactgg gtgccgggga tgcccgtacg gtcaagctcc
     7921 tcacacgggc cggtatgggc tggtgccagg ggcagacctg cgggttcgcg gtccgtgagc
     7981 tcatcggccc ggcggcgtcc ggggcgggcc ccggaaggca ggccctcgcg tgcccggtcc
     8041 tcgtgtccgt actcgccggt catccgcccg ccaccggcgc ggcgggcgac tccgacgcgg
     8101 ccgaaaccgc aggtggggcg ggcgcaacag gcggttccgg cgggacgggc tgaggtccac
     8161 gggcccggcc gacacctata cgtacaccct tgtgctcggt gaccgcgcac cgcggccatc
     8221 agtgacatgt cacatctccc aaggagtttc gatgcagacg aacgacaccc ggacgtcccc
     8281 ccgaacccgt ccgtggcgcg gtgtcatggt cgccacgccg ctgccgctga aggaggaccg
     8341 ctccgtcgac ttcgacgcgt tcgccgaaca cgtcggccgc ctcgtcgagg cagggtgcga
     8401 cggggtggtc cccaacggtt cgctggggga gtaccagacg ctcaccgacg acgaacgcga
     8461 ccggctggtc cgcgtggccg tggcggcggc gggcgacggt gcccgggtca tgcccggagt
     8521 ctccgcctac ggcagcgacg agtcacggcg ctggaccgag caggccgccg acgcgggcgc
     8581 gggatccgtt ctgctcctgc ctcccaacgg ataccgctgc gacgacaccg cgatccgtgc
     8641 ccactacgcg gcggtcgccg aggtcgggct gccggtcgtc gcctacaaca acccgtacga
     8701 caccaaggtg gatctcaccc cccggctgct ggcccgtctc catcaggagg gcagcatcgt
     8761 ggcggtcaag gagttcagcg gtgacgtccg tcgcgcgtac gagatcgccg aggaggcccc
     8821 cggcctcgat gtcctcatcg gtgcggacga cgtcctgctc gaactcgcgc tggccggagc
     8881 cgtcggctgg atcgcgggct gtcccaacat gctgcccgcg agctgtgtca cgttgtaccg
     8941 cgccgccgtg gccggagacc tgggcaccgc actgccgctc taccggcagc tgcacccgct
     9001 gctgcgctgg gactccaagc cggagttcgt ccaggcgatc aaggcgtcga tggatctggt
     9061 ggggtggcac ggcggcccct ccaggccgcc gcggttcccg ctgcccgaag cctccgccgc
     9121 cgccgtgcgg gcagccaccg agaaggcgct cgccgagggc ctgaactgac gccctcccgt
     9181 ctgcctcgtg tccgtcatct ccccgcaccg gcggggccgt tcaagggggg cccttgcgta
     9241 cacgtcgtat cttccacgcc gtcgactcgc acaccgaggg aatgcccacg cgggtgatca
     9301 ccggtggggt cggcaccatt cccggtgcga ccatggccga gcggcgccgg tacttcctgg
     9361 agcaccagga cgcggtgcgc acgctgctga tgtacgaacc gcgcggacac gccgccatga
     9421 gcggcgccat actccagccg cccacccggc cggacgcgga cttcggcgtc ctgtacatcg
     9481 aggtgtccgg agcgctgccg atgtgcgggc acggcaccat cggtgtggcc accgtgctgg
     9541 tggagaccgg catggtggcc gtcaccgaac cggtcaccac cgtccggctg gacacccccg
     9601 ccggactggt cagcgtcgac gtccaggtcg aggacggggc ggcgacggcg gtcaccttca
     9661 ccaacgtgcc cgccttcagc gccggtctcg accgcaaggt cgacgtcccc ggccacggaa
     9721 tcgtgcatta cgacctggcc ttcggcggca acttctacgc catactcccg ctggccgacc
     9781 tcgggcttcc cttcgaccgc gcacgcaagg acgacattct ggcggccgga ctcgcggtga
     9841 tggacgcgat caacgccacc gaccccccgg tccacccggc ggaccccgat atccgtggcc
     9901 tcaagcatgt tcagctcctc gccccgggct cggacgccgc gcactcccgt catgccatgg
     9961 ccatccaccc cggctggttc gaccgctcac cctgcgggac gggtacctcc gcacgcatgg
    10021 cgcagctgca cgcccgcggc caactgccgc tgaacaccga cttcgtcaac gaatcgttca
    10081 tcggtacctc gttcaggggg aggcttgtcg aggagaccag tgtcgccggc accccggccg
    10141 tcgttcccac ggtgacgggc cgggcctgga tcaccggcac cgcgcagttc ttcctggatc
    10201 cgaccgatcc cttccccgaa ggcttcctgc tgtgagagtg ccgggggacg ggccgtacgg
    10261 tccggtggcg agcgcttgtc ggcggggggc gaatgagcaa cgtgacattg tattctgagg
    10321 ccccgctcgg tgctggagga atacatatga cccgcctctc atccctcaac accatctcgg
    10381 cccaggagca tctgagggac cgggtggccc acgcgctgag ggcggcactg atcgccggtg
    10441 acctgcgtcc gggcacgatc tactccgcac ccgcactggc ctcggagttc ggagtctccg
    10501 cgaccccggt acgtgaggcc atgctcgacc tggtccgcga gggcctggtc gaggccgtac
    10561 gcaacaaggg tttccggatc accgagttga ccgaccagga cctcgacgac ttcaccgaac
    10621 tccgggcgat gatcgaggtt cccactgtcg gcaggatcgc cgggctcggc aagtcccggc
    10681 agttggaggc cctgcggccc ctggcgctgg agatcgtcca cgcggcacgc cggcacgaca
    10741 tcctggccta cctggaggcc gaccggcgct tccaccttga gctgctcggc ctggccggga
    10801 accggcggct ggtcgaagag gtcagcaatc tgcgcaagcg ctcacggctc ttcggtctca
    10861 acaggcttgc ggaatcaggc cagttgatcg catcggccga ggagcacatc gaactgctcg
    10921 acctgatcgt cgcgggagac gcgaaacgcg ccgaggcgtg catgctcgct catatgtcac
    10981 atgtccggtc gctctgggcc gatggtggag aagtgggcga gggcgagggt gtcgagcgca
    11041 tcactcatcg acgggtgggc cgggggacgg cgggggcctg agcgagtgtg cctccgccct
    11101 ttggggtggg ggcacccgag agcgctcacc cggcccaccg ccgacgagtg tcagatcggg
    11161 ttgaggcggg ccttgagcag acagaactca ttgccttcgg ggtcggtcag gacgtgccac
    11221 tgctcctccc ccgtctggcc gatgtcggcc gggcgcgcac cgagcttcag caggcgttcg
    11281 agctcggcgt cctgatcgcg gtcagtggcg ttgacgtcga tgtgcagccg ggacttcccc
    11341 ttctccggct cgtcccggcg actgaggatg atcgtcggct gcggaccacc gaacccttcg
    11401 cgcggcccga tctccaccat ttcgccgtcc tcgcgatcga gcacgacgaa gtccagaacc
    11461 tcgcaccaga accgtgccag cgcctcaggg tcgtggcaac cgagcacgag ctcactgata
    11521 cgacatgcca tggatgaaac ctgctctcag cctgggaatc gccggggaag ccacacgcga
    11581 aacccatgga ctcccggcaa ggaaaacaat cccgcgaccg taccggacgg ggtgagcggg
    11641 ggcgaagtga tttcgaggcc gcccctgtcc gggcagccgg gccgggccgc cggccatgcg
    11701 ggaggggtgc gggctgtgag ggacggccca cggggcagcc cctagagatg tgactcggcg
    11761 gaccagtcgc ctcagggcgc gttctgtcgg atctcgccct gccccattgg gtggccgtgc
    11821 gtcgcgttgt cggcctccgg agccgggccc gcgcgggagt cggagcgcgg accccgtgcg
    11881 ccccgccgcg tcggcgtacc tgctcgccgt tcctgtcgtg cgggcctcgt gcccgacggg
    11941 agcgatcatc ggcgacagcc gctgccgggg gctcacgaat cgcctgctgg gcaaccgagt
    12001 tggcgcgcag atgaatgtgg gcacggcatc ttccaaagaa catgtgacat gtgccattgt
    12061 actgatcgca gggtgtgagg tcgtgctcat gtcacacgga gccaccgtca actccgttac
    12121 tgccagcccc tgttaaaccc tccacagccg cgctccccgg cgcggcgacc actccgctgg
    12181 gaggcggcac gtgaagaacc gatcggttcg accaagattt accggcacat ccaccggcgt
    12241 atccacccgg acatcggcat cagcccgcat atccaccggg atatccaccg tgatgctggc
    12301 ggcctgcctg ggagtagggc tgctcgcggc cccggcccag tccgcgccga cgcggtccac
    12361 caccgccacc gccgccccgt ccgacgcggc aacccccgtc gtcccggacc cgaacggtcc
    12421 tgcggcggcg cccgcgaccc agcccgccga ggaccccgag cacctcggcg ccacgccgct
    12481 caagccggcg gaccgggccc cgcagtccgc gtccaaggac gtgctcaagc gcgactacga
    12541 cgattcccgg gccgccgaac ccagccgccg cgcaccctcg atgtcggcca aggccaggct
    12601 tgccgccaag gcgcgcggac tcgccgctcc cgcggcgtgc aatgtcagtg acttcaccag
    12661 ccgtaccggc gacgcactgg tccagcaggt gacctcgacc accaccgagt gcgtcaacac
    12721 gctgttcggc gtcaagggcc aggacggcta tctcgccttc cgcgaggcgc agatggtcac
    12781 catcgccgat gccatgcgtg cgcgcacagc ggcctatccc ggcgacagca gcaccgggat
    12841 gccgcagttg gttctcttcc tccgggccgg ctactacgtg cagtactacg acccgtcgac
    12901 ggtcggcagc tacggtccgg cgctccgcac cgcgattcag ggcgccctgg acggcttctt
    12961 cgcctcctcg cactcctcgg acgtcaccga cgcgaacggg gagacccttt ccgaagcggt
    13021 catcctgatc gacagtgccg agcagaacga ccgctatctg catgtcgtca agcgcctgct
    13081 cgcgagctac aactcctcgt acaacagctc ctggtacatg ctcaatgccg tcaacaatgt
    13141 gtacacggtg aca
//
