LOCUS       NZ_LWLE01000002        11422 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold10, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000002
VERSION     NZ_LWLE01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 400740)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400740)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 86960
            Orig. end                         :: 98382
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11422
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11422
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11422
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6422](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6422)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            481..2041
                     /gene="hemG"
                     /locus_tag="A6A29_RS06995"
                     /old_locus_tag="A6A29_25490"
                     /pseudo=""
     CDS             481..2041
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956908.1"
                     /locus_tag="A6A29_RS06995"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25490"
                     /product="protoporphyrinogen oxidase"
                     /pseudo=""
                     /sec_met_domain="DAO (E-value: 6.4e-09, bitscore: 30.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRSHQRVNTRTGHVVVIGGGIAGLAAAHRLVLAGLRVTLLEATE
                     RLGGKLMTGEIAGTRVDLGAESMLARRPEAVGLARAVGLGDRLQPPATATASVWTRGAL
                     RPMPKGHVMGVPGDPAALSGVLSPEGLARIAQERDLTPTAVGDDVAVGSYVADRLGREV
                     VDRLVEPLLGGVYAGDAYRISMRAAVPQLFEVVKEGGSLLDGVRRIQERAAARQQTGPV
                     FQGIDGGVGTLPDAVAAAVRAEGGEILTATPVLGLTRGDEGWNVRTDTRVITADGIVLA
                     TPAWSASTLLAAESPAASAELAGVEYASMALVTLAFRRCDIGEAGALHGRSGFLVPPVD
                     GRTIKASTFSSNKWQWVADAAPGLFVLRTSVGRYGEEDHLHREDDELIDVSLRDLAEAT
                     GLAAKPLDTQVTRWIGGLPQYPVGHLDRVARIRDEVAKLPGLRVCGAVYDGVGIPACVA
                     SAHRAADELAHDLTGEGAGEGTAAVTGTVTDGSAGEDAGGDHGRDHRHVDPGSGHSERG
                     GTI"
     gene            2046..2774
                     /gene="hemQ"
                     /locus_tag="A6A29_RS07000"
                     /old_locus_tag="A6A29_25495"
     CDS             2046..2774
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="A6A29_RS07000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25495"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073719100.1"
                     /transl_table=11
                     /translation="MSAPETATSSKGPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLQAKDITVRGTYDVSGLRADADLMIWWHAETADELQEAYNLFRRTKL
                     GRAVEPVWSNMALHRPAEFNKSHVPAFLADETPRNYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWVLAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSIADLVAGLA"
     gene            complement(3039..3812)
                     /locus_tag="A6A29_RS07005"
                     /old_locus_tag="A6A29_25500"
     CDS             complement(3039..3812)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432059.1"
                     /locus_tag="A6A29_RS07005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25500"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073719101.1"
                     /transl_table=11
                     /translation="MLWVMFLLVAWGAAAISCVRLCLASAEAAHPLADVAREPARHELN
                     LYETAFLAGGPHRVADLALVSLHLRRRLLLAHTGWATVVDPEGVDEVERTVIRAIGPEG
                     QSRIAPVRAAAAAADAVRTLADRLVAAGLAVPPGTRTGIVSAVRAVRGSAVLVVVMGAA
                     AVLIPGREPGYGGPMPAWFGLPLVLTLGCLAIARIENHPYSPWASPTGQRWLDSLPAPA
                     RGADRELLAAVAVRGVRAVRDPALRAALTGRPMNA"
     misc_feature    3848..3866
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.76"
                     /tool="antismash"
     gene            complement(3903..4994)
                     /locus_tag="A6A29_RS07010"
                     /old_locus_tag="A6A29_25505"
     CDS             complement(3903..4994)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049031.1"
                     /locus_tag="A6A29_RS07010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25505"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_073719102.1"
                     /transl_table=11
                     /translation="MNVLALLLSLAVAVSSVLLLTRTGRDRGRRRPTDSDVFVHDLYEA
                     AFLNGGPARVVDTALTALYSDGRLIIGGPGIVAVQRPEARDPVERAVLSSLADAPSGAL
                     HTLRDAVMRHPAVQEVGDGLAARGLLVSPGESLPRRRWGLIQGLSCLVALPVSLMLTFV
                     QYAMHEGYADYPVPFIVTMLPAILIGGLIGLSVTLAARNRITKEGLRAMHGYRSAHAYA
                     MGPAHLVATMGLGALPDPVLQGQLMAAARFRTGNRRSASFRTASGAGAPSHSSGTSDVL
                     AATVWCAGASPSGASCGGSTGGGGHSGGGGGCSSGTSCSSGSGSSCSSGSGSSCSSGSS
                     CGGSSGGSSCGGSSGGSSCGSSS"
     gene            complement(5001..6422)
                     /locus_tag="A6A29_RS07015"
                     /old_locus_tag="A6A29_25510"
     CDS             complement(5001..6422)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072529.1"
                     /locus_tag="A6A29_RS07015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25510"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073719103.1"
                     /sec_met_domain="DUF692 (E-value: 6.2e-93, bitscore: 305.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGIGIGWRPEIADAVEALPGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVSLGLGGADRPDAGRLADLAARAELLGTPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALEVLCENVRIAQDSLPVPLALENIAALISWPGEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPVEAIAYVHVAGGIEKDGVWHDTHA
                     HPVTRPVLDVLTELRSRVDPPGVLLERDDDFPPATELADELTMIRATLKAAAATGTATP
                     RAASPRDTAAPKAADAPYTVAPRTVAPRTATAVDGPAAPKDPDAASASGPVRTGCAPAV
                     RERAALAQTALLSALVAGTPAPEGFDQRRLGVQSRALAAKRAGVVAKVAPELPEILGDD
                     YRDAYLAYAMTRPMSAGYRRDALDFAEQLLIAGRPADAAARRRLTHWWQDRAAPRPPRR
                     TTRLVRAARAVLVGK"
     misc_feature    complement(6402..6417)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.66"
                     /tool="antismash"
     gene            complement(6639..7379)
                     /locus_tag="A6A29_RS07020"
                     /old_locus_tag="A6A29_25515"
     CDS             complement(6639..7379)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="A6A29_RS07020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25515"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_073719104.1"
                     /transl_table=11
                     /translation="MSSNDTPAPDPATTAPDSPFRTEPTSRDEAPQFVLPLVLHLEKTD
                     PPARTDALRTAARAVLTILSDERSLGEGEWAQEMRDWQDARIRKVVRRARGAEWRKASA
                     LPGITVTGEGAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPPAAPDTTGPVLWLNPE
                     LDMSAGKTMAQAGHGAQLAWWELSDTERKAWREAGFPLAVATADAERWRRLTVSGLPVV
                     RDAGFTEIAPGSCTVVADHPALRG"
     gene            7473..8147
                     /locus_tag="A6A29_RS07025"
                     /old_locus_tag="A6A29_25520"
     CDS             7473..8147
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="A6A29_RS07025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25520"
                     /product="AIM24 family protein"
                     /protein_id="WP_073719341.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQQATAPGMTLQNAKSIKYAVNGEMHARQGSMVA
                     YRGNLQFERKGQGLGGMLKRAVTGEGLPLMAVRGQGEAWFAHEAANCFIVEMEQGDVLT
                     INGRNVLCFDASLTYEIKTVKGAGMTGGGLFNSVFSGYGKLGLMCEGHPIVIPVTGQQP
                     VYVDTDAVVGWSEQLSTSLHRSQSFGSMVRGGSGEAVQLMLQGEGFVIVRPSELKPEKT
                     AN"
     misc_feature    8426..8441
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
     gene            8644..8835
                     /locus_tag="A6A29_RS07035"
                     /old_locus_tag="A6A29_25530"
     CDS             8644..8835
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="A6A29_RS07035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25530"
                     /product="hypothetical protein"
                     /protein_id="WP_073719105.1"
                     /transl_table=11
                     /translation="MDDAYCETPAPALVRAPGPAAVGPPYAECVLCREPTEYPESTRGT
                     TLCPVCEWQEAGRTACSG"
     gene            complement(8947..9783)
                     /locus_tag="A6A29_RS07040"
                     /old_locus_tag="A6A29_25535"
     CDS             complement(8947..9783)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049037.1"
                     /locus_tag="A6A29_RS07040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25535"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_073719106.1"
                     /transl_table=11
                     /translation="MTALTLLGVVLAGCGAPTDSGTEANPSHAPSHASSVAPPPSPSKP
                     PTLAPGPAGRTPVFERGLPHGRGDKVVALTFDADMTADQGARAAAGEQFDNPPLIALLR
                     RLKVPSTVFMTGRWAEEYPEQARSIGTDALFEIANHSYSHHAFASPCYGLPVVDRDGML
                     GDVEQAFAAFRKAGARNVVPYFRFPGGCYDDASLRALAPAKVTAVQWDVISGDAFATDA
                     DAVAEQVLDGVRPGSLVVMHCTRSAAPVTEEAVRQVVPELLRRGYRFVKVSELMRD"
     gene            complement(9839..9961)
                     /locus_tag="A6A29_RS47475"
     CDS             complement(9839..9961)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6A29_RS47475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_274535790.1"
                     /transl_table=11
                     /translation="MDDSPQPRDTHPVDRRPPLEKLAVQVGSAERGIRSAVNIS"
     misc_feature    complement(10479..10493)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 gccgccgcga cggccttcgg gacgctcttc acctcgacgg acagggtggc cgtgcggatg
       61 acatgggtgg cggcggcccc cgccttcggt gccgcggagg ccctcccggc gggcttcgcg
      121 ccgtccgcgg acttcgcgcc gtccgccgcc ggaccggctc cggcctgttc cccgcccccc
      181 gccctgtcgt ccgcgtccgg cgcctgcagt cccgccgcgc tggtgtcgga ggagctttcg
      241 tcgctcgcgg agccgcagcc gccgacggcg agcagggcga cgagcgcccc ggtggcgagg
      301 accgcgcggg aacggcgcgt gcccggacga gaggtgtttc ttgcggtttt cataacgggt
      361 ccccccagga cgatggttga tggtgccggt tcgacgtacg gaccccctgc gcgggttgcc
      421 ggtcagcggt ttcgaagtgg tcacggtcat gactcggtac gggtttgaga gagtggaccc
      481 atgcagcgtt ctcatcagcg cgtgaacacg cgtacgggcc atgtcgtcgt catcggcggt
      541 ggcatcgccg gtctggccgc cgctcaccgg ctggtcctgg ccggactgcg ggtcacgctc
      601 ctggaggcga cggagcggct cggcggcaaa ctcatgaccg gggagatcgc gggcacccgc
      661 gtcgatctcg gcgcggagtc gatgctcgcg cggcgccccg aggcggtcgg cctggcccgt
      721 gccgtcggcc tcggtgaccg cctccagccg cccgccaccg ccaccgcctc ggtatggacg
      781 cggggcgccc tgcggccgat gcccaagggc catgtcatgg gcgtgccggg cgatccggcg
      841 gcgctcagcg gggtgctctc cccggagggc ctcgccagga tcgcccagga gcgcgacctc
      901 accccgaccg ccgtcggcga cgatgtcgcg gtcggcagct atgtcgccga ccggctcggc
      961 cgcgaggtcg tggaccggct cgtggaaccg ctgctcggcg gggtgtacgc gggtgacgcc
     1021 taccggatct cgatgcgggc cgccgtaccg cagctcttcg aggtggtgaa ggagggcggc
     1081 tccctgctcg acggggtgcg gcgcatccag gagcgggccg ccgcccggca gcagaccgga
     1141 cccgtcttcc agggcatcga cggcggcgtc ggtaccctgc cggatgccgt cgccgccgcc
     1201 gtacgggccg agggcggcga gatcctcacc gcgaccccgg tcctcgggct cacccgtggc
     1261 gacgagggct ggaacgtccg caccgacacc cgggtgatca ccgccgacgg catcgtcctg
     1321 gccacccccg cctggtccgc ctccacgctg ctggccgccg agtccccggc cgcctccgcc
     1381 gaactggccg gagtcgagta cgcgtcgatg gccctggtca ccctggcgtt ccggcgctgc
     1441 gacatcgggg aggccggtgc gctgcacggg cgctcgggct tcctcgtccc gccggtcgac
     1501 ggccgcacga tcaaggcgtc caccttctcc agcaacaagt ggcagtgggt ggccgacgcg
     1561 gcgcccggcc tcttcgtcct gcgtacctcc gtcggccgtt acggcgagga ggaccatctg
     1621 caccgcgagg acgacgaact gatcgatgtc tcgctgcgcg acctcgccga ggcgaccgga
     1681 ctggccgcga agcccctcga cacccaggtc acccggtgga tcggcggcct tccgcagtac
     1741 ccggtcggcc acctcgaccg ggtcgcccgc atccgcgacg aggtcgcgaa gctgccgggg
     1801 ctgcgggtct gcggggcggt gtacgacgga gtcggcatcc ccgcctgcgt cgcgagcgcg
     1861 caccgggccg cggacgagct cgcccacgac ctgacgggag aaggcgcggg agagggcaca
     1921 gcggcggtca cgggaacggt caccgatggg agcgcgggag aggacgccgg gggcgatcac
     1981 gggagagatc atcgccacgt cgaccctggt tcggggcact cggagcgagg cgggacaata
     2041 gccgtatgag tgctcctgag actgcgacat caagcaaggg tccgaacgcc ggcaagaagg
     2101 ccaaggacct caacgaggtc atccgctaca cgctgtggtc cgtcttcaag ctgcgcgatg
     2161 tgctgcccct ggaccgcgcg ggctacgccg acgaggtcca ggagctgttc gaccagctcc
     2221 aggccaagga catcaccgtg cggggcacgt acgacgtgtc cggtctgcgg gccgacgccg
     2281 acctcatgat ctggtggcac gccgagaccg ccgatgagct ccaggaggcg tacaacctct
     2341 tccggcgcac caagctgggc cgcgcggtgg agccggtctg gtcgaacatg gcgctgcacc
     2401 gccccgccga gttcaacaag tcgcacgttc cggcgttcct ggccgacgag acgccgcgca
     2461 actacatcag cgtctatcct ttcgtgcgct cctacgactg gtacctgctg ccggacgagg
     2521 accgtcgtcg gatgctcgcg gaccacggca agatggcccg cggctacccc gacgtccgcg
     2581 ccaacaccgt cgcctccttc tcgctcggcg actacgagtg ggtcctcgca ttcgaggccg
     2641 acgagctgta ccgcatcgtg gacctgatgc gtcacctgcg ggcctccgag gcgcggctgc
     2701 acgtccgcga agaggtgccg ttctacacgg ggcgcaggaa gtccatcgca gacctggtgg
     2761 ccgggctcgc atagccagcg tttcccgacc accccaaccc ggaagatcag acgacgggca
     2821 ccggcaccgc gtgtgccgcc cgccgttcca gcgacaccgg gttcggctcc gggaacgccg
     2881 caggccgcct gcctgcggcg ttcccgggac cggcgaggtg gccggacaac cgccgccgaa
     2941 caaccaccgt cgaacagcag ccgccgaaca accaccgtcg aacagcagcc gctgaacagc
     3001 agccgtcacg ggagtgccca cctcctcccg cccacggctc aggcgttcat cgggcggccc
     3061 gtcagagccg cccgcagtgc cgggtcccgc acggcgcgga cgccgcgtac cgccaccgcg
     3121 gccagcagtt cgcggtccgc gccccgcgcc ggggcgggca gcgagtccag ccaccgctgc
     3181 ccggtggggg aggcccacgg gctgtacggg tggttctcga tcctggcgat ggcaagacag
     3241 cccagggtca gtacgagcgg cagcccgaac caggccggca tcggaccgcc gtaccccggc
     3301 tcccggcccg ggatcagtac ggcggcggcg cccatcacca ccacgagcac ggccgaaccg
     3361 cgtacggccc gcaccgccga cacgatgccc gtgcgggttc cgggcggcac ggccagcccc
     3421 gcggcgacca gccggtcggc cagcgtgcgt acggcgtcgg cggcggccgc cgcggcccgt
     3481 accggcgcta tccgggactg cccctccggg ccgatggcac ggatcacggt ccgctcgacc
     3541 tcgtccacgc cctcgggatc gacgacggtc gcccagccgg tgtgggcgag cagcagccgc
     3601 cgccgcagat gcagggagac cagcgccaga tcggcgaccc ggtgcgggcc gccggccagg
     3661 aaagccgtct cgtacaggtt cagttcatga cgtgcgggtt cccgcgcgac gtcggccaaa
     3721 ggatgcgcgg cctcggcgga ggcgaggcag agccggacac aggagatcgc cgctgcgccc
     3781 cacgcgacca gcagaaacat gacccagagc atgccggatt tctatgcgag gcaggcggcc
     3841 gggcgccatg ccctgttcag tatgcggacg ggtgcctgtc cgacggcctg tccgacgggc
     3901 ggtcaggagc tgctgccgca gctggagccg cccgagctgc cgccacaact cgatccgccg
     3961 ctgctgccgc cgcagctgga cccggaactg caactggacc cggaccccga actgcaactg
     4021 gacccggaac cggaactgca gctcgtaccc gaactgcagc ccccgccacc gccgctgtgc
     4081 ccgccgcccc cggtcgagcc cccgcagctc gcaccgctgg ggctcgcacc ggcacaccac
     4141 accgtcgcgg cgaggacgtc cgacgtaccg gaggagtggg agggggcacc ggcaccggaa
     4201 gccgtacgga acgacgccga ccgccggttc ccggtacgga accgtgccgc agccatcaac
     4261 tgcccctgga gcacgggatc gggcagcgcg cccagtccca tcgtggccac cagatgggcc
     4321 gggcccatcg cataggcgtg ggcggaccgg tagccgtgca tggcccgtag cccctccttc
     4381 gtgatccggt tacgggcggc caacgtgacg gacaggccga tcaggccgcc gatcaggatc
     4441 gcgggcagca tcgtgacgat gaacgggacc ggatagtccg cgtagccctc gtgcatcgcg
     4501 tactggacga acgtcagcat cagggagacc ggaagggcca cgaggcagct cagcccctgg
     4561 atcagccccc agcggcggcg cggcaggctc tccccggggg agaccagcag cccacgggcg
     4621 gcgagtccgt ccccgacctc ctgcaccgcc ggatgccgca tcaccgcgtc ccgcagtgtg
     4681 tgcagcgccc cgctcggtgc gtcggccagc gacgagagca cggcccgctc gaccgggtca
     4741 cgggcctccg gccgctggac ggcgacgatg ccggggccgc cgatgatcag acgtccgtcg
     4801 gagtacaggg cggtcagcgc ggtgtccacc accctggccg ggccgccgtt caggaaggcc
     4861 gcctcgtaca ggtcgtgaac gaagacgtcc gagtccgtgg gccgtctccg gccgcggtca
     4921 cgccctgtcc tcgtcagcag cagcaccgag gagacggcca ccgcgaggga cagcagcaga
     4981 gccaatacgt tcatgacgga tcacttcccc acgaggacgg cacgggccgc ccggaccaga
     5041 cgggtggtac gacgcggcgg gcggggggcc gcccggtcct gccaccaatg ggtcagccgc
     5101 cgcctggccg ccgcgtccgc cggccggccc gcgatcagca gctgctccgc gaagtcgagc
     5161 gcgtcacgcc ggtatccggc ggacatgggc cgggtcatgg cgtacgcgag gtaggcgtcc
     5221 cggtagtcgt cgccgaggat ctccggcagc tccggcgcga ccttggcgac cacccccgcc
     5281 cgcttggcgg ccagcgcccg gctctgcacg cccaggcgcc gttggtcgaa gccctcgggt
     5341 gcgggagtgc ccgcgacgag cgccgagagc agcgcggtct gggcgagggc ggcgcgttcc
     5401 cggaccgccg gggcgcagcc ggtgcgcacc ggcccagagg ccgacgcggc gtccgggtcc
     5461 ttcggggcag ccggtccgtc cacggccgtc gccgtcctcg gcgccaccgt cctcggcgcc
     5521 accgtgtacg gcgcgtccgc tgccttcgga gcagctgtgt ccctcgggga cgccgccctc
     5581 ggagtcgccg tgcctgtcgc ggctgccgcc ttcagggtgg cgcggatcat ggtcagttcg
     5641 tccgccagct ccgtcgcggg cgggaagtcg tcgtcacgct ccagcagcac accgggcggg
     5701 tcgacccgtg agcgcagctc ggtcagcacg tccaggacgg gccgtgtcac ggggtgggcg
     5761 tgcgtgtcgt gccagacgcc gtccttctcg atgccgcccg ccacatggac gtacgcgatg
     5821 gcctccaccg gcagttcgtc gagcgccttc gcggggtcct cgccgcggtt gacgtggttg
     5881 gtgtgcagat tggccacatc gatcagcagc cgtaccccgg tgcgctcgac cagctccgcc
     5941 aggaactgcc cctccgtcag ttcctcgccc ggccaggaga tgagcgccgc gatgttctcc
     6001 agggccagcg gcacgggcag cgagtcctgg gcgatccgca cgttctcgca cagcacctcc
     6061 agcgcgtccc aggtccgcgg caccggcagc agatggcccg cctccagcct cggcgacgcg
     6121 gtgagcggcc ccccggcccg tacgaacgcg atgtgctccg tcaccagcgg cgtaccgagc
     6181 agttccgccc gcgcggccag gtccgcgagc cgccccgcgt ccgggcggtc ggcgccgccc
     6241 aggcccaggg agacgccgtg cggcacgacg gtgacgccac gctcgcggag ccgtaccagg
     6301 gagtcgggca gatggtcggc gcagaggttc tccgccaccg cctcgaccca gtcgatgccc
     6361 ggcagcgcct ccacggcgtc ggcgatctca ggccgccagc cgataccgat tcccagtctc
     6421 atggtgtccc ccttgtccat cccgtgcagg ggtaatggcc ccggcaggcg gtggtgaatc
     6481 cgaacacagg gacgttcaga ggtcgatttg aggttccccg tccccgggct ccggtgcggc
     6541 ctctcccgca ccgcccgcct cccgcagaga gggccgggag ggccgggagg gccgggagag
     6601 ccggaggccg gagggccggg agggaccgcc ccggcggatc agccgcgcag cgccgggtgg
     6661 tcggcgacca ccgtgcagga ccccggggcg atctcggtga acccggcgtc gcgcaccacc
     6721 ggcagtccgc tcacggtcag ccggcgccag cgctccgcgt cggcggtggc cacggcgagc
     6781 gggaaaccgg cctcgcgcca cgccttgcgc tccgtgtccg acagttccca ccaggcgagc
     6841 tgcgccccgt gcccggcctg cgccatggtc ttgcccgccg acatgtccag ctccgggttg
     6901 agccacagca ccggacccgt cgtgtcgggt gcggccgggg gctccgggtc gtccaggtcc
     6961 gtgcccgaaa cctggagctt ggccagctcc ttgggccagc cgtccagagg caccggcggg
     7021 aacacccgta cctcggcgcc ctcgcccgtc accgtgatcc cgggcagtgc ggacgccttg
     7081 cgccactccg cgccgcgcgc ccggcgcacc accttgcgga tccgggcgtc ctgccagtcc
     7141 cgcatctcct gcgcccactc gccctcgccc agggagcgct cgtcggacag gatcgtgagc
     7201 accgcgcggg cggccgtccg cagggcgtcg gtacgggccg gcgggtccgt cttctccaga
     7261 tgcagcacga gcggcagcac gaactgcggt gcctcgtcgc gggaggtcgg ctcggtccgg
     7321 aacgggctgt ccggggcggt cgtggcggga tcgggcgcgg gggtgtcgtt gctgctcacc
     7381 gtcccagtct gccaggccct cccatgcccg aattccggcg gaggaatcac gcttcttggc
     7441 ggaatgacat gctccgggtg aggatgctgc gcatgaagag cgatctcttt tccagcgagc
     7501 acatggccca gcaggccacc gcccccggta tgaccctgca gaacgccaaa tcgatcaagt
     7561 acgccgtcaa cggggagatg cacgcccgcc agggatcgat ggtcgcctac cgcggcaacc
     7621 tccagttcga gcgcaagggc cagggcctgg gcggcatgct caagcgcgcg gtcaccggcg
     7681 aggggctccc gctcatggcg gtccgcggcc agggcgaggc atggttcgcc cacgaggcgg
     7741 ccaactgctt catcgtggag atggagcagg gcgacgtcct gaccatcaac ggccgcaacg
     7801 tcctctgttt cgacgccagc ctgacgtacg agatcaagac cgtgaagggc gccggaatga
     7861 ccggcggcgg cctcttcaac agcgtcttca gcggctacgg caagctgggc ctgatgtgcg
     7921 agggccaccc catagtgatc cccgtcaccg ggcagcagcc ggtgtacgtg gacacggacg
     7981 cggtcgtggg atggagcgag cagctctcca cctcgctgca ccgctcgcag agcttcggct
     8041 ccatggtgcg cggcggctcc ggcgaggccg tgcagctgat gctccagggc gaagggttcg
     8101 tgatcgtacg gcccagcgag ctcaagcccg agaagacggc caactgagac cgaccggcac
     8161 atggggagcg gtctgaacct gaggggcgtc ggacgccgtt acggcctcgg cggaccgtgg
     8221 gtgctgcgcg gagtcgatct cgacctgccc gcgcggagac cgcaccaccg acatccccct
     8281 gccgtccgcc gcactcgcgg gactgctcgc cgtcctctgc tgcgccctgc tgggagcggc
     8341 tgtcggcgcg ctctgcaccc ggccgctgct gaaccgccgg ggctggtccg tcgcggccac
     8401 cgttctcggc gtcctgctcg ccctggtcac cagcggctca ccggcgaagt acgcggtcac
     8461 ctcgctggtc tccggatcgc gtaccggctc ggtctccgtg cccggagtgc cgctgctcgg
     8521 ggcggcggtt ctcgcggcgg cggtggctct cctcgtctgc cggtcggccg cgcggtgcgg
     8581 gtgacgacgg gcgcgtgccc cgcacatccc cggcgggcgc gggcggcccg tacgctcgta
     8641 cccatggacg atgcgtactg cgagactccc gccccggcct tggtgcgggc gcccggcccg
     8701 gccgccgtgg gccctccgta tgccgagtgc gtgctgtgcc gcgagccgac ggagtacccg
     8761 gagtcgaccc gcggcaccac cctgtgcccg gtctgcgagt ggcaggaagc gggccgcacg
     8821 gcctgttccg gctgaccgct gccccatccg ggcgctcttc tcggggcccg cctccgtccg
     8881 gccccgcccg gccccgtccg cctccgtccg gccccccggc cggccccgtc cggctcccgt
     8941 gcgggctcag tcgcgcatca gctcggagac cttcacgaag cggtagccgc gcctgagcag
     9001 ctccggcacg acctgccgta cggcctcctc ggtgaccggt gccgcgctgc gcgtgcagtg
     9061 catcacgacc agcgaccccg gccgcacccc gtccagcacc tgctcggcca ccgcgtccgc
     9121 gtccgtggcg aacgcgtcgc cgctgatgac gtcccactgc accgccgtca ccttcgccgg
     9181 tgccagggcg cgcagcgacg cgtcgtcgta acagccgccg gggaagcgga agtacggcac
     9241 cacattgcgt gccccggcct tccggaacgc ggcgaaggcc tgttccacat cgccgagcat
     9301 cccgtcccgg tccaccaccg gcaggccgta gcagggcgag gcgaaggcat ggtggctgta
     9361 cgagtggttg gcgatctcga acagcgcgtc cgtgccgatg gaccgcgcct gctccgggta
     9421 ctcctcggcc cagcgcccgg tcatgaagac ggtcgagggg accttcagcc ggcgcagcag
     9481 cgcgatcagc ggcgggttgt cgaactgctc gcccgccgct gcccgcgctc cctgatcggc
     9541 cgtcatgtcg gcgtcgaagg tgagtgccac gaccttgtca cctcgaccgt ggggcagtcc
     9601 ccgttcgaag accggtgtcc ggccggccgg acccggcgcg agagtgggcg gcttcgaggg
     9661 agacggcggg ggcgcgaccg aggaggcgtg cgagggggcg tgcgaggggt ttgcctcggt
     9721 accggagtca gtaggggccc cgcaaccggc gagtactacc cctagaaggg tcagagcggt
     9781 caaacgcgta actttccgtt caaatagggt cactgacgga aagtaactga tgtgatgatc
     9841 aggagatgtt cacggcgctc cggatgcctc gttcggccga ccccacctgc accgccagct
     9901 tctccagggg cgggcggcgg tcgacgggat gcgtgtcccg aggctgcggt gaatcgtcca
     9961 tgacatgccc tccgtgctgg tcccagcggt ggagagcatt gagccaggcg ttgttcgagg
    10021 gcgtgcggcc gttcgattac accgctgtta cgggggtggc gcgggcgatc cgcaccaccc
    10081 ccggtgaggg ctcagccggt cagcaccacg gcagcccgct cggtccccgg tccgctcagc
    10141 gccagggtcc ggtgaccgcg aacgtcgtgc ccggcgtgta gcagttgacg aacatcgtcc
    10201 cgccgtccgg ggagaaggtg acgcccgcga actcgcccca ctccggctcc tcgggcgtcc
    10261 cgatgttctg ccgcccgcgc gccatggcgt acacctcgcc gcggcgtgtc accccgagga
    10321 cgtgctgggc gccgcccccg tcctcacaca ccatcagccc gccgtcggag gccaggcaga
    10381 tgttgtccgg ggactcgccg ggcagttgga tgtcggtgtc gggaccgaag acgacgacca
    10441 gcgtgagccg gcgcttcttc ggctcgtacc gccacacctg gccgaagtgg tcggccgcgg
    10501 agccctccga gtggcgggcg aagctggaga cgaagtagac gcaggagccg ccccagtaac
    10561 agccctccag cttctgggcg tgggtgatgc ccttcggccc gaagtcctgg agccggatcg
    10621 gagtctcggc ggccagcgga tcgggtacgg gaacccactc gacccggtcg aagctcgcac
    10681 ccgtctcctg gacggcggag agatcggcca gacccggcac ccgcatggcc tccagcgcgc
    10741 caccggcccg cagcgagccg gtgccgccca gcggcttctc gggcaggaag cggtagaaga
    10801 gcccgaacgg cttgtcgaac gcgtcctccg tctcgtagac gaccccgttc ctcggatcga
    10861 tcgcgatggc ctcgtgctgg aaacggccca tcgcggtcag cggaacggcc ccggtgcggc
    10921 gcggatctgc gccgtccacc tcgaagatga agccgtggtc cttggtgtac ccgttggtcc
    10981 cggccttgtc ctcggtctcc tcgcaggtca gccaggtgtt ccagggggtg gggccgcccg
    11041 cgcagttcac cgccgtaccg gcgatggcga cgcgctcgcc gagcactctg ttgcggccgt
    11101 ccagttccag ggccgtgcag ccgcccttgc ccatggggtc gtaggtgagg ccctcgacgg
    11161 tcgggacgcc gatcctgccg gtgaccctgt tctcgtggtt gcggacgaga tggacgcggc
    11221 cgtgacggcc cgcgaaagcg gccataccgt cgtggttgct gggtacccgg ccctcgccgg
    11281 agcgcagcgg gtcgccctcc cgtgacagca cccggtagcg gaagcccttc ggcagatcga
    11341 ggaggccgtc cgggtccgtg acgaggggcc cgtagccgct gtgtccccgt gcggcggcgg
    11401 tccccgcgaa gagttcggag aa
//
