LOCUS       NZ_LWLE01000002        11368 bp    DNA     linear   CON 27-MAR-2024
DEFINITION  Streptomyces sp. TSRI0281 scaffold10, whole genome shotgun sequence.
ACCESSION   NZ_LWLE01000002
VERSION     NZ_LWLE01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. TSRI0281
  ORGANISM  Streptomyces sp. TSRI0281
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 400740)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400740)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905125.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:37:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,600
            CDSs (total)                      :: 8,518
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 273 of 345
            Pseudo Genes (internal stop)      :: 33 of 345
            Pseudo Genes (multiple problems)  :: 102 of 345
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:13:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4584
            Orig. end                         :: 15952
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWLE01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:6368](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6368)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    20..34
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(1670..3409)
                     /locus_tag="A6A29_RS06675"
                     /old_locus_tag="A6A29_25185"
     CDS             complement(1670..3409)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955801.1"
                     /locus_tag="A6A29_RS06675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25185"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_073719052.1"
                     /transl_table=11
                     /translation="MMLHPELLHAARGARRPLGGATALLAAVTATHLTQAVLLAVVLSR
                     IARGETAQLLPPLASVLAVVLVRAALGRAQRLIAVTAGAAVRVRLRDTLLERVGSLGPT
                     AITGTRAGAVRATLVDGVEGVDAYISRYLPQLLITCAIPPLLLTAVALVEPYAALVLGP
                     ALLLALFGPRWWNRLLDRRGKEHWDSYEALAADYLEALQGMPTLRAAGAVGRVRERLER
                     RSAAVHRTTVAKLRVSLLDTGLTDLAVQGGTVAAVLVACSSATTGRTAPTGTYVLLLLA
                     SECFRPIRDLSREWHAGYLGVSAADGIAALRTAEAAVPDHGAVRAAWQAAPEICFEDVR
                     FTHPGAGRPALDGVSFTAAAGRTTAVVGPSGAGKSTLLSLLLRQRDPDSGRVTVAGTDT
                     AAYTLASLRRGIAVVSQETYLFHATIAENLRIARPAATDEQLHAAARAAGIDQEIARMP
                     DGYATLVGERGATLSGGQRQRLALARALLADSPVLILDEATSAVDERGEARIVRELLRA
                     GRGRTCLVVAHRLDAVRHADHILVLDAGRVAAAGDHTSLLAGGGVYASLVAAGRTAREE
                     RAA"
     gene            complement(3406..4986)
                     /locus_tag="A6A29_RS06680"
                     /old_locus_tag="A6A29_25190"
     CDS             complement(3406..4986)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377866.1"
                     /locus_tag="A6A29_RS06680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25190"
                     /product="nitroreductase"
                     /protein_id="WP_241838360.1"
                     /transl_table=11
                     /translation="MPLCPAPPAPAISGAAPAARAFDPAPASGPVLPLASPRSGSGSGS
                     EQSAPDPLRHALADARSVRVPGLSPYVPELPLPWGGPALPLTGPGAAIDLDRTLRLSLA
                     GPAGTPGRLRPVPSAGALHPVRAHLLVGAGCSLPPGRYAYDPRTHRAHRRGPAPGDAPP
                     GALAVLTVAAARTVAHYGHRAWPLLLLDAGHAAAALALAGAPAGARVSLDADGALLSAA
                     AGLPPVAEWEDAWPGTEPELPLAAVRLAPARVPGARHDPGATALSAWAALPPAAAPLPQ
                     PGAESPPLRESAAARYLLDRLTAAPCRPGGTWHPVTRPEAVTDDTLRRRRSAAPDALTR
                     TPDREALARILATAQTARPDGPAWTLAVGGTAPALYTTAVSGTDLDIAAVGDARPTLAR
                     WAACQQWVGSAGAVLLAHGCPEDAAPALVRSSHLAAGYAVGAAHVHATALGLGSRPIGS
                     WQQADLGAALGDTPRHNWIVHGLALAAPPGGPKPAVIAMCRKPAERTAPAPPATPSAPT
                     ALPGEEERP"
     misc_feature    complement(4240..4242)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5001..6368)
                     /locus_tag="A6A29_RS06685"
                     /old_locus_tag="A6A29_25195"
     CDS             complement(5001..6368)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="A6A29_RS06685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25195"
                     /product="YcaO-like family protein"
                     /protein_id="WP_079182634.1"
                     /sec_met_domain="YcaO (E-value: 2.3e-44, bitscore: 146.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVLPIEALVDPVSGIIRDVAPVEHPTGAPPRYTAMTADVADAR
                     RLGAWPADRVSLGTTFGDPGGAWIATVAEAVERYCGNRLPPRGHPRAPRRATAAELAAE
                     GHRVYGPGALPTYAPWQYDRRGFPYAELTPDTPALWTEGTENGRSCWAPVALTHLNWRQ
                     GELRSLPRTHHLNYAGIATGQGFDDAVERGLLEVVERDALELWWHLDGPTRGIAPASVP
                     GLTEDLADCGLEVHIVEMPSEFAPSMAALVHDPARGIHAAGFACRYNPAEAARKAVLEA
                     VHTWVFTQGAVDADGWVYRAVEAGMLARGLYLDHRADRRYLDDCGTEFAAVRDLGAHIQ
                     VWLDERMTPLARRFTDPARGTVPITSIAPGSRTALDDALTAGGHRVITADLTTEDVAET
                     TLRVARVLVSGLVPNAPAAFGYFGCPRFADAALARGWRSQPPSEAKDFTLAPPPHM"
     gene            complement(6365..8017)
                     /locus_tag="A6A29_RS06690"
                     /old_locus_tag="A6A29_25200"
     CDS             complement(6365..8017)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377868.1"
                     /locus_tag="A6A29_RS06690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25200"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_073719054.1"
                     /transl_table=11
                     /translation="MNTRTAYVPAPDGTPLATDVCLPDTTGPHPAVMIRTPYGRTAHRA
                     ELRGWAAHGFAALAQDVRGRHGSPGEWHPYQGHEETDGLATATWVREQAWSSGEVVASG
                     ASYAAHCALVTALAATEDGRPDAVIAAVPALGLADTAREHAGPERLWARAGWWAAHGDR
                     PDSDPDALERILADDPHVLEHLPIARLAERLAGGLGRELPSWSRLWADRTRGQIVERGA
                     TARLPLLAVGGAHDPFAEDTVALWHGWGGPARLLLGPWGHRLTADPTTSGRRSVNLGAL
                     YVRWARAALSGGLEPNRRGAIALGDSGRWHSTPEPTGFGAAGGKAARLPVHEGCCGPCA
                     ETAHTPHPDAEPVPEPEPDGPRASAWAFGTATGLRLLHGAEFTADPARPVRSDDLTVPA
                     DGDPADRCLLLSPPLPRPLDLAGAAEARINVTADAPSADWAVRLTALDPAGRAEPLAFG
                     IVRRTDPPGSAVHVTVPLGTLGRRLPAGTRLRAEIAGHHFPAHARNPHTGEDPVTATRL
                     VPSRRTVTAQGSSVLLPVIARRRYVEPVPEICR"
     gene            complement(8014..8901)
                     /locus_tag="A6A29_RS06695"
                     /old_locus_tag="A6A29_25205"
     CDS             complement(8014..8901)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452727.1"
                     /locus_tag="A6A29_RS06695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25205"
                     /product="hypothetical protein"
                     /protein_id="WP_073719055.1"
                     /transl_table=11
                     /translation="MTRSRLAPGTGIVTVPGAGPVLRTSAGEFLRIDMGGVDGGVLVDR
                     LTAGETVPSEGTNSAALDRLVAAFEAEGHTLSTPRSAPLTGRRVHVLGDPVLTRPLARC
                     AAAEGAEVHHSTADRVAELARDPAGHDRLAVVWCLDSPVPEGLWDTADRLPGRRTGWLR
                     CHREGAHSWIEPLAAAPDDVTSRHVRLRRLAATAAHRELAAYWAGHRTPDTGPHPTEAS
                     AALIAALLTADLIAWASGETGPHREGGLPARRRMRRIDLRDLSVTEHPVLPVPDVAPLP
                     VPASAETGTAKARP"
     gene            complement(9009..9143)
                     /gene="amiA"
                     /locus_tag="A6A29_RS46270"
     CDS             complement(9009..9143)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="A6A29_RS46270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_015613003.1"
                     /transl_table=11
                     /translation="MEHNKVFSPIADQGQLAHLSATHSNALVENPFDDAVEADTADEK"
     misc_feature    9208..9221
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.45"
                     /tool="antismash"
     misc_feature    complement(9208..9223)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.88"
                     /tool="antismash"
     gene            complement(9209..10072)
                     /locus_tag="A6A29_RS06700"
                     /old_locus_tag="A6A29_25210"
     CDS             complement(9209..10072)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129276.1"
                     /locus_tag="A6A29_RS06700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25210"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_073719056.1"
                     /transl_table=11
                     /translation="MAGQSGTVRAEGLGPDVVRAEGLGFGYPGREVLRGVDLRIGAGEL
                     VALIGLNGCGKSTFLKLAAGLLTPSSGRVLLEGDDASRLPRRTAARRAALLHQSAPAVP
                     GLTVRQLVRQGRYATRGPLGMLREGDDAVTCRALADVGVTAWADRPVDALSGGERQRVR
                     LAMALAQDAHVLFLDEPTTYLDLRHQLDVLQTVVRLRAERRLTVVMVLHDLNHAARFAD
                     RIVALREGRIAADGPPGEVVTRRLLAEVLGVEGRVGIDSEGGWPICYPDHPVHPPQPLW
                     NENHIH"
     gene            complement(10168..11217)
                     /locus_tag="A6A29_RS06705"
                     /old_locus_tag="A6A29_25215"
     CDS             complement(10168..11217)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613001.1"
                     /locus_tag="A6A29_RS06705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A29_25215"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_073719057.1"
                     /transl_table=11
                     /translation="MTAPEDRPARRAPRVTGVRMGGLAVLVAAVACADLFAGRGVTPEG
                     VRAVFLGGGDPVTEHIVLQLRLPRLLVALAAGASLGVAGLVLQSALRNPLAGPEVTGVT
                     PGAVLGAVGATGLGLAGWDSPTAVVVAACLGGFAGAGLLWLLAGREKGDPEQTAIYGVL
                     VSAVLAGITAVVLLVAPGELGSVVQWLIGSTEGRVWQHWNLLWPWAAVWGGAAWLLAGP
                     LTLLRCGDEQAGAAGLDTGRGRGAALVCAVALTAGAVSAVGALGFVGLLVPHLALAVFG
                     ADLRITLPGAALLGAAVVCGADAAAQLLSRLLATALEADRLTLPVGALTTFVGAGLLLA
                     VVRRQAEPR"
ORIGIN
        1 cgggtggcgg agtcggctgc gagcgcctcg gccagttccg cgtcaccccg gtgatccaga
       61 tgggccgtcg tctcgtccag gaccagtacg cgcgggctcc ccagcagccc ccgggccagg
      121 gcgacccggg cgcgctgccc cccggagagg gtcgccccgc gctcgcccac ggcggtgtcc
      181 agtccgtggg gcagcgcgtc cgcgatgggg tcgatgccgc agaggcgggt caccgcgtcc
      241 agttcctcct gcgtcgcttc cgaagcgccc agacggaggt tctcggcgag ggtgccgtgg
      301 aagagcggcg cctcctgccc gaccacgcag acggcggccc gaaggtccac ctcggtaagg
      361 tctcgcaggt ccacggggtg cgcgggggac ggaggaagga gttccacggt tccctcccgg
      421 gggtcccagt agcgcgccaa gaggtgcgca caggtcgtct tgcccgcccc ggaggctccg
      481 accagggcca cggtctcgcc gggccggacg atcaggtcga ggccgtccag gacggtgtct
      541 ccgccgtagc cgaaccgtat gccgcgcagc cgaaggccca gcgggcccgc cggaacgggg
      601 cgcggatcgg cgggttcggg aactccggcc ggtgcgtgga cggcggcccg gacccgtgcc
      661 gcagcggccc tcaggctgcc cgcccgcccg agtgcggccg ccgattcggc gaccggcgcc
      721 agggcgccca gtgcgagggc catggcagcc ggggcccagg cccggtccag gtgtccggcg
      781 gacgcggcgt gggcagccgc cgcgacgacc gcgatgacgg cggcgacgac cagcaggtcg
      841 cgggccgcgc ccgcaccggt ctcccaggac tgttcggcgc gctgagcccg tgcgagtcgg
      901 cgtccggcgg tccggagccg ggcccgtctg cggttcaggg cgcccgccat caggagttcg
      961 cgcagtccgt cgacgcattc cacggtctcg gcggagacct cggcgagcgc ggtccgggtg
     1021 cgggctccgc gctcggcgcg cccgcgcgct tcgagcaggg gcgaccagat cagcagcaga
     1081 gcggcgggca ggacgcccag cagcagccac ggcccgagcg ccgccagcac agtggccgac
     1141 acggcgaaga ccactccgga ggcgagaagt tgggcgatgg cgtgggcgta gaagaactcc
     1201 agcgcctcaa cgtccccgag agcggtcgct gcgaggtcgc cgctgcgccg acccgcgatc
     1261 cgggcgggag cgctgcgggc gagtccgtcg aagacccgga cgcgcagttc ggcgagcacc
     1321 cggtaggcaa ggtcgtgcga gaggtccatc tcgcgccagg tggcgagggc acggagcagg
     1381 acgagcccga ccaggacggc gacggtccag ggcgcgggcg ggcggtgctc ggtgacggcc
     1441 gtgccgaccg tgtgcgcggc gagcgcggcc agggcgacca gggccgcttg atcgacgagc
     1501 gcggccagac aggtgcgcag cacggtcgtg cggtgcgcgc cgaggaccgg gaggagcgag
     1561 cgcagggcgc cggttccgcc atcgaagaca gctccggttg gtggggctgt gtcgggggct
     1621 tcggcggctc cggaggaccc gtccggggcg ggccggtgcg ggggcggtgt catgcggcgc
     1681 gctcctcccg tgcggtgcgc ccggccgcga cgagcgaggc atacacgccg ccgcccgcga
     1741 gcagggacgt gtggtcgccg gccgcggcga cgcggccggc gtcgagcacg aggatgtggt
     1801 cggcgtggcg tacggcgtcg agccggtggg ccacgacgag gcaggtacgc cctcgtccgg
     1861 ccctcagcag ttcccgtacg atgcgcgcct cgccgcgttc gtccaccgcg ctggtggctt
     1921 cgtccaggat caggaccggc gagtcggcga gcaacgccct tgccagggcg agacgttggc
     1981 gctgtccgcc ggatagcgtg gcgccgcgtt cgccgacgag ggtggcgtaa ccgtccggca
     2041 tccgggcgat ctcctggtcg atgccggccg cgcgggcggc ggcgtgcagt tgctcatcgg
     2101 tggcggcggg gcgggcaatg cgcaggtttt ccgcgatcgt ggcgtggaag aggtaggtct
     2161 cctgcgagac gacggcgatg ccccggcgca acgaggcgag ggtgtacgcg gcggtgtcgg
     2221 tcccggcgac ggtgacgcga ccactgtccg ggtcgcgctg tcgcagcagc aggctcagca
     2281 gggtcgactt cccggcgccg gagggtccga cgacggctgt ggtacgcccg gcggccgcgg
     2341 tgaaggatac gccgtcaagg gcgggccgtc cggccccggg gtgagtgaag cgcacgtcct
     2401 cgaaacagat ttcgggggcg gcctgccagg cggcccgcac ggccccatga tccgggacgg
     2461 cggcttcggc ggtgcgcagg gcggcgatgc cgtcggcggc ggacactccg aggtagcccg
     2521 cgtgccactc gcgggagaga tcgcggatcg ggcggaagca ctcggaggcc agcaggagca
     2581 ggacgtaggt gcccgtgggg gcggtgcggc cggtggtggc cgaggagcac gccacgagga
     2641 cggcggcgac cgtgccgccc tggacggcca ggtcggtgag gccggtatcg aggagcgaga
     2701 cccggagttt ggcgacggtg gtccggtgca cggcggccga acgccgctcc agccgctccc
     2761 gtacacgccc cacagcacct gccgcgcgca gggtcggcat tccctggagg gcctccaggt
     2821 agtccgcggc gagggcttca taggagtccc agtgctcctt gccgcgcctg tcgaggagac
     2881 ggttccacca gcgcggtccg aagagggcga gcagcagggc gggcccgagt acgagggccg
     2941 cgtacggttc gacgagggcg acggcggtga ggaggagcgg cggaatggcg caggtgatga
     3001 ggagttgggg caggtagcgc gagatgtacg cgtcgacgcc ctcgacgccg tccaccaggg
     3061 tggcgcgcac ggctccggcc cgtgtgccgg tgatcgcggt ggggccgagc gagccgacgc
     3121 gttccagcag ggtgtcgcgg agccggaccc gcacggcggc ccctgcggtg acggcgatca
     3181 ggcgctgggc gcggccgagg gcggcgcgta cgaggacgac ggcgaggacg gacgcgagcg
     3241 gcggtaggag ttgggccgtc tcgccgcggg cgatccggga gagcaccacg gccagcagga
     3301 cggcctgggt gagatgggtg gcggtcacgg ccgcgagcag cgcggtggca ccgccgagcg
     3361 ggcggcgggc gccacgggcg gcgtgcagga gttcggggtg gagcatcatg ggcgttcttc
     3421 ctcgccgggc agggccgttg gtgcggaggg tgtggccggt ggtgccggtg ccgttcgttc
     3481 tgccggcttt cggcacatgg cgatgacggc aggtttcggg ccgcccgggg gtgcggctag
     3541 ggccaggccg tgcacgatcc agttgtggcg gggggtgtcg ccgagcgccg cgccgaggtc
     3601 ggcctgttgc caggagccga tgggtcgtga gccgagtccc agcgcggtgg cgtggacgtg
     3661 ggcggcgccg accgcgtaac cggcggcgag gtgactgctg cggacgaggg cgggcgcggc
     3721 gtcctcgggg cagccgtgcg cgagcagcac cgcaccggcg ctgcccaccc actgctggca
     3781 cgcagcccac cgggccaggg tgggtcgcgc gtcgccgacg gcggcgatgt caaggtccgt
     3841 gccgcttacc gccgttgtgt acagggccgg ggcggtgccg ccgactgcga gggtccacgc
     3901 cggtccgtcc ggccgggctg tctgcgcggt cgccaggatg cgggcgagcg cctcccggtc
     3961 aggcgtccgg gtcagtgcat cgggtgcggc gctccggcga cggcgcaggg tgtcatcggt
     4021 cactgcctcg gggcgcgtga ccgggtgcca ggtcccaccg ggccggcagg gtgctgcggt
     4081 gaggcgatcc agcaggtagc gtgctgcggc ggattcgcgc agtggggggc tttccgctcc
     4141 gggctggggg agcggggcgg cggcgggggg cagagcagcc caggcgctca gcgccgttgc
     4201 gccgggatcg tggcgtgcgc cgggtacgcg cgctggggct aaccggactg ccgcgagcgg
     4261 aagttccggc tcggtgccgg gccaggcgtc ctcccactcg gcgaccgggg gaagtcctgc
     4321 ggcggcggac agcagggccc cgtccgcgtc gagggagacc cgggcgccgg ccggggcccc
     4381 ggccagcgcc agggcggcag cagcatggcc cgcgtccagg aggagcagcg gccaggcccg
     4441 gtgaccgtag tgcgcgacgg tgcgggcagc cgccacggta aggacggcga gggcgccggg
     4501 tggcgcgtcg ccgggggccg gtccccgccg gtgggcgcgg tgggtgcgcg ggtcgtaggc
     4561 gtaccgtccg ggtggcagcg agcagcccgc cccgaccagc aggtgcgcgc gcaccgggtg
     4621 cagggcgccg gcggagggca ccgggcgcag ccgccccggc gtaccggcgg gcccggccag
     4681 ggacagccgt agcgttcggt ccaggtcgat cgccgcgccg ggacctgtca ggggaagcgc
     4741 cgggccgccc cacgggagcg ggagctcggg gacgtacggg gagaggcccg gtacgcgcac
     4801 cgaccgggcg tctgcgagag cgtgacgcag ggggtccggt gctgactgct cggagccgga
     4861 gccggagccg gagcgcggcg aggcgagggg aagtacggga ccggaggcgg gggcgggatc
     4921 gaaggctcgg gcggcgggag ccgcgccgga gatcgccgga gcggggggcg cgggacagag
     4981 aggcatggcg gggtgctcct tcacatgtgc ggcggcggag cgagggtgaa gtcctttgct
     5041 tcggaaggcg gttgactccg ccagccccgg gcgagcgcgg catcggcgaa gcgtgggcag
     5101 ccgaagtagc cgaaggccgc cggtgcgttg ggaaccagtc cggagaccag cacgcgggca
     5161 acgcgtagcg tggtctcggc aacgtcctcc gtggtgaggt ccgcggtgat cacccggtgc
     5221 cccccggcgg tgagggcgtc atcgagcgcg gtgcggctgc cgggggcgat gctggtgatg
     5281 ggtacggtgc cccgcgcggg atcggtgaag cggcgggcga gcggcgtcat ccgctcgtcg
     5341 agccacacct gtatgtgtgc gccgaggtcg cgcacggcgg cgaactcggt tccgcagtcg
     5401 tcgagatacc gccggtcggc ccggtggtcg aggtacaggc cccgggcgag catcccggcc
     5461 tccaccgccc ggtacaccca gccgtcggcg tccaccgcgc cctgggtgaa cacccaggtg
     5521 tgcaccgcct ccagtacggc cttgcgggcc gcctccgccg ggttgtaccg gcaggcgaac
     5581 ccggccgcgt ggatgccgcg cgcggggtcg tgcacgaggg cggccatgga gggggcgaat
     5641 tcggagggca tctccacgat gtgcacctcc aggccgcagt ccgccagatc ctcggtgagg
     5701 ccggggacgc tcgccggggc gatgccccgg gtgggcccgt cgaggtgcca ccacaactcc
     5761 agggcgtcgc gctcgacgac ttcgaggagt ccgcgctcga cggcatcgtc gaaaccctgt
     5821 cccgtggcga tacctgcgta gttgaggtga tgggtgcggg gaagtgaacg cagttcgccc
     5881 tgacgccagt tgaggtgggt cagggcgacg ggggcccagc aggaccgccc gttctcggtc
     5941 ccctcggtcc acagtgccgg tgtgtcgggg gtgagttcgg catacgggaa gccgcggcgg
     6001 tcgtactgcc aaggggcgta ggtgggcagc gcgccgggtc catagacgcg gtggccctcg
     6061 gcggcgagtt cggcggcggt ggcgcgccgg ggcgcgcggg ggtggccgcg cggcgggagg
     6121 cggttgccgc agtagcgctc gacggcctcg gccacggtgg cgatccaggc gccgcccggg
     6181 tcgccgaagg tggtgcccag cgagacccgg tcggccggcc aggcgcctag gcgacgggcg
     6241 tcggccacgt cggcggtcat cgcggtgtac cggggcggcg caccggtggg gtgttcgacg
     6301 ggcgcgacgt cgcggatgat cccgctgacg gggtcgacga gggcttcgat gggcaggacg
     6361 ctggtcaacg gcatatctcc ggtacgggct cgacgtagcg gcgtcgggcg atgacaggca
     6421 ggaggacgga gctgccctgg gcggtcacgg tcctgcggga cgggacgagc cgggtggccg
     6481 tgaccgggtc ctcgccggtg tgtgggttgc gggcgtgggc ggggaagtgg tgcccggcga
     6541 tctcggcccg tagtcgggtc ccggcgggca gtcgacggcc cagcgtgccg agcggcacgg
     6601 tgacgtgcac ggcacttccg ggcgggtcgg tgcgccggac gatgccgaag gccagcggtt
     6661 ccgcgcggcc tgccgggtcg agtgcggtca ggcggacggc ccagtcggcg gacggcgcgt
     6721 cggccgtgac gttgatccgg gcctcggcgg ctccggccag gtcgagcggg cggggcaacg
     6781 gcggcgagag gagcaggcag cggtcggcgg gatctccgtc ggccgggacg gtgaggtcgt
     6841 cggagcgcac ggggcgggcc gggtcggcgg tgaactcggc tccgtggagc agtcgcagac
     6901 cggtggccgt accgaaggcc caggcggagg cccggggccc atccggctcc ggctccggca
     6961 ccggctccgc gtccgggtgt ggggtgtgag cggtttccgc gcacggaccg cagcagcctt
     7021 cgtgcacggg aagacgggct gctttcccgc cggcggcccc gaaccccgtc ggctccgggg
     7081 tactgtgcca gcgcccgctg tcgccgaggg cgatggcccc gcgccggttc ggttccagcc
     7141 cgcccgacag agcggcgcgg gcccaccgta cgtacagcgc accgaggttg acgctcctgc
     7201 ggcccgacgt ggtcgggtcg gcggtgaggc ggtgccccca gggccccagc agcaggcggg
     7261 ccgggcctcc ccagccgtgc cacagggcca cggtgtcctc ggcgaacggg tcgtgggcgc
     7321 ccccgacggc gagcagcggg agccgggcgg tggcgccccg ctcgacgatc tggccccttg
     7381 tgcggtcggc ccacagccgg gaccaggacg gcagttcgcg tcccagcccc ccggcgagac
     7441 gttccgccag gcgggcaatc gggaggtgtt ccagcacatg ggggtcgtcc gccaggatgc
     7501 gttccagcgc gtccgggtcg gagtcggggc ggtcgccgtg ggcggcccac cagccggcgc
     7561 gggcccacag ccgctcgggg ccggcgtgct cgcgtgcggt atcggcgagg ccgagggcgg
     7621 ggacggctgc gatgacggcg tcgggacgtc cgtcctcggt cgcggcgagg gcggtgacga
     7681 gggcgcaatg cgcggcgtag gacgcgcccg aggcgaccac ctcgccgctg ctccaggcct
     7741 gttcgcgcac ccaggtcgcg gtcgccaggc cgtctgtttc ctcgtgcccc tggtacggat
     7801 gccactcccc gggcgagccg tggcgaccgc gtacgtcctg ggcgagggcg gcgaatccgt
     7861 gcgccgccca gccgcgcagc tcggcgcggt gggcggtccg cccgtacggg gttctgatca
     7921 tgacggcggg atgggggccg gtcgtgtccg gaaggcatac gtcggtggcg aggggcgtgc
     7981 cgtccggtgc gggaacgtac gcggtgcggg tgttcacggg cgtgccttcg cggtcccggt
     8041 ttccgcggat gccgggacgg gtagcggggc cacgtccggg acggggagta cggggtgctc
     8101 ggtgacgctg aggtcgcgca ggtcgatccg ccgcatccga cggcgggcgg ggaggccgcc
     8161 ctcccggtgc ggcccggttt caccgctcgc ccaggcgatg aggtcggcgg tgagcagcgc
     8221 ggcgatcagt gcggcggagg cttcggtggg gtggggtccg gtgtcggggg tgcggtgtcc
     8281 ggcccagtac gcggcgagtt cgcggtgggc ggcggtggcc gcgagtctgc gcaggcgcac
     8341 atggcgggag gtgacgtcgt cgggggccgc ggcgaggggc tcgatccagc tgtgggcgcc
     8401 ctcgcggtgg cagcgcagcc agcccgtccg acggccgggc agtcggtccg ccgtgtccca
     8461 cagcccttcg gggacgggcg agtcgaggca ccagaccacc gccaggcggt cgtggccggc
     8521 ggggtcccgg gcgagttcgg ccacgcggtc ggcggtggag tggtgaacct cggccccttc
     8581 cgccgcagcg cagcgtgcca gtggtcgggt caggacgggg tcacccagga cgtggaccct
     8641 gcgtcccgtg agcggggcgc tgcgcggggt ggacaacgtg tgcccctcgg cctcgaacgc
     8701 ggccaccagc cggtcgagtg cggcggagtt cgtgccctcg gacgggaccg tctcccctgc
     8761 ggtcagccgg tccaccagga ccccgccgtc gacaccgccc atgtcgatgc gcaggaactc
     8821 cccggcggag gtgcggagca cgggcccggc accgggaacg gtgacgatcc cggtgccggg
     8881 agcgagtcga ctgcgggtca cgggtacctc cggtgcgatg gctgggtggc tgaacgggac
     8941 gggcccgccc gggaattgac cgggcgggcc cgccgcgtgg gtgcgggtac ccgcgtgagc
     9001 gggtgtccct acttctcgtc cgcggtgtcg gcctcgacgg cgtcgtcgaa ggggttctcg
     9061 acgagggcgt tcgagtgggt ggcggagaga tgggcgagct ggccctggtc ggcgatcggg
     9121 ctgaacacct tgttgtgctc catgagtgaa gcctccattc gtatcggatg ggtgttgggc
     9181 ggcgttggtg cgtgccgcag cacagacact aatgaatatg attttcattc cacaagggtt
     9241 ggggtggatg aacagggtga tctgggtaac agatgggcca gccgccctcg ctgtcgatcc
     9301 ccacccggcc ctccacaccg aggacttcgg ccagcagacg ccgggtgacg acctcgccgg
     9361 gaggcccgtc ggccgcgatc cggccctcgc gcagcgcgac gatccggtcg gcgaaccggg
     9421 ccgcgtggtt gaggtcgtgc agcaccatca ccaccgtcag tcgacgctcc gcgcgcagcc
     9481 gtacgaccgt ctgaagcacg tccagttggt gccgcagatc gaggtaggtg gtcggttcgt
     9541 ccaggaagag aacgtgggcg tcctgggcca gcgccatggc cagtcgcacc cgctgtctct
     9601 ctccgccgga cagcgcgtcc accggccggt ccgcccaggc ggtgacgccc acgtccgcga
     9661 gcgcccggca cgtcaccgca tcgtccccct cccgcagcat tccgagcggt cctctggtgg
     9721 cgtaacgccc ttggcgcacc aactgccgta cggtcaggcc cggtacggcc ggtgccgact
     9781 gatgcagcag cgcggcccgt cgggcggcgg tgcgacgggg cagccgcgaa gcgtcgtcgc
     9841 cttcgagcag cacgcgccca ctgctcgggg tgagcagtcc ggcggcgagc ttgaggaagg
     9901 tgctcttgcc gcagccgttc agcccgatca gggcgaccag ttcaccggca ccgatgcgca
     9961 ggtcgacccc gcgcagcacc tcgcgtcccg ggtagccgaa gccgagtccc tccgcccgga
    10021 cgacgtccgg gccgagccct tccgcccgga ccgtgcccga ctgtcctgcc atgcctctct
    10081 cctcatccat cggctcctgt ttcggcgtcg cactcacgcc tcgccatgtc cgcacccctg
    10141 tacggcgcat gtccgcccct ggccaggtca tcgcggctcc gcctgtcggc ggaccacggc
    10201 cagcagcaac cccgccccca cgaaagtggt cagtgcaccg accggcaggg tcagccggtc
    10261 cgcctcaagt gccgtggcca gcagtcggga cagcagttgg gcggcggcat ccgccccgca
    10321 caccaccgcc gcgccgagca gcgcggcgcc cgggagtgtg atgcgcaggt ccgcaccgaa
    10381 gacagccagc gccagatgcg gcacgagcag ccccacgaaa ccgagcgccc ccacggccga
    10441 gacggcgccc gccgtcagcg ccaccgcaca cacgagcgcc gcccctctgc cccggccggt
    10501 gtccagaccg gcggcgccgg cctgttcgtc gccgcagcgc agcagggtca gcggtccggc
    10561 aagcagccag gccgcaccgc cccagaccgc cgcccacggc cagagcaggt tccagtgctg
    10621 ccagacccgc ccttcggtgg aaccgatcaa ccactgcacg acgctgccga gttcacccgg
    10681 ggccaccagc aggaccacgg cggtgatccc ggcgagcacg gccgacacga ggaccccgta
    10741 gatggccgtc tgctccggat ctcccttctc gcgcccagcg agcagccaca gcagacccgc
    10801 tcccgcgaag cctccgaggc aggccgccac gaccacggcc gtgggcgaat cccagcccgc
    10861 cagaccaagg ccggtggcac cgaccgcgcc gagcaccgcg cccggggtga caccggtgac
    10921 ctcgggtccg gccagcgggt tgcgcagggc cgactggagg accagcccgg ccacgcccag
    10981 actggccccg gcggccagcg ccaccagcag gcggggcaac cgcagttgga gcacgatgtg
    11041 ctccgtgacc gggtcgccgc ccccgaggaa caccgcccgt acgccttcgg gggttacgcc
    11101 ccggccggcg aacagatcgg cgcaggcgac cgcggcgacg agcaccgcca ggccgcccat
    11161 ccggactccc gtcacgcgcg gggcccggcg ggccggccgg tcttctgggg cggtcatcgt
    11221 tccgccttcc gcagggtgag gacatccgac ggactccccg gaacggacac ggccacaggt
    11281 ttcgattgcg gggcgggccg cctcctcccg cgcttgagca gcaccacccc gacgggcaca
    11341 ccgatcaggg ctgtccacgc accaacgg
//
