LOCUS       NZ_LWKZ01000028        11290 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold8, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000028
VERSION     NZ_LWKZ01000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 308580)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308580)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 294148
            Orig. end                         :: 305438
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11290
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11290
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11290
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:6290](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6290
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(1078..1087)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.63"
                     /tool="antismash"
     misc_feature    complement(1080..1089)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     misc_feature    1084..1097
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.02"
                     /tool="antismash"
     gene            1155..2705
                     /locus_tag="A6A06_RS42435"
                     /old_locus_tag="A6A06_32165"
     CDS             1155..2705
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /locus_tag="A6A06_RS42435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32165"
                     /product="transposase"
                     /protein_id="WP_199931426.1"
                     /transl_table=11
                     /translation="MTTETAHPATRTPARPLSAPLSSSHPLAVRTAPDKSGVRDVSGAS
                     ADTHTAGSGRTARPGRSTAHNGGTGRTSAAGTTGPAGRTGTVRRPGRADAPAAPAPSAA
                     ASGDSASTAATVTSGPSETFDAFAEDLFGHLPRTDQRRWAHAYLLALLTTEGKKSVRRL
                     AAAVSDSPTASQSLHQFLNASPWDWNPVRREIVRRAEQPAPPRAWILAPAVLPKRGGHS
                     VGVHRRFDSAVGRTVSCQLAMGMFLPVGDAPTAAAVSASAAATSAVTAASAASSVAASV
                     AASAAGDCGGSAAVSVDWRLFLPPQWAEETELLRRNRIPASETSHPRPMWVHALDLVEA
                     QAARSTCPPAPVVADLSEYSDSVRLIEALARQGRDFVIAVPPATPLATPATPGMPGDPG
                     TRPRLLQSAPTPVRLPGSHHTYRAFAEPGPANARPPRVWLTNMAHRRMGQLVGLARLHQ
                     LPAATLRTLATDFGLHDFEGRSFPGWHHHMTLASAAHAYRALAAEPDEATLSTVRFVS"
     gene            complement(2725..4020)
                     /locus_tag="A6A06_RS39405"
                     /old_locus_tag="A6A06_32170"
     CDS             complement(2725..4020)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133276.1"
                     /locus_tag="A6A06_RS39405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32170"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_079194653.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.1e-06, bitscore:
                     22.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAASMADDVSGGVSATAPRRVVVTGATGFIGSAVMRELLRAATGP
                     TAPGDRPRPDPEPYGSTSHPEGSTSHPGVNQNPDSVSGTETGPRHLDPNPRPYPDAHSH
                     SLHLRATARKPPEAPQPAGAPVEWVAADLADPASLRGVCEGADVLLHLASDIGRDEERC
                     ELVNVRGTAALVAEAVRAGVGRIVMLSTAAVYGAGPHAGPGVDEVTPAPVSAASRTRLA
                     AERPVLEAGGTVLRPGLVLGAGDRWVVPALAELLGRVPARWDGGRGLLSAVAVDDLARL
                     FARLALAPDGIPGGVHHASHPVPVRNGDLMDTLIGHGVFPQPKGTLATPQNDMPGMPGT
                     PQNGTPQNATPPNGIAEALPDLPWDECLKRLGSTPGRVSARQFALLASDHWYRSDEVWT
                     AAGCDPGPGPLVRLAEAADWYRSHLQDRARAR"
     gene            complement(4170..4784)
                     /locus_tag="A6A06_RS39410"
                     /old_locus_tag="A6A06_32175"
     CDS             complement(4170..4784)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986960.1"
                     /locus_tag="A6A06_RS39410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32175"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073768901.1"
                     /transl_table=11
                     /translation="MQERAVRTREVILRAAAEVFDEFGFSGASISKIMKRAGVTQGGMY
                     FHFPSKEALAQAVMIEQGGGLELPPGEDGLQRLVDITLFLAEELQSNPTLRAGVRLAVE
                     QGEFGVQDDSAYRHWAEEFGGQLRAAREKGELLPEVNVDELSWVLVSAYTGTQLFSQIS
                     SGRADLHQRVVSLWRYLLPGVAAPPVRPQVTFAARRRRAAA"
     misc_feature    complement(4816..4829)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.42"
                     /tool="antismash"
     misc_feature    4817..4832
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.36"
                     /tool="antismash"
     misc_feature    complement(4819..4832)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.26"
                     /tool="antismash"
     misc_feature    complement(4823..4836)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.63"
                     /tool="antismash"
     misc_feature    complement(4824..4837)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.94"
                     /tool="antismash"
     misc_feature    complement(4825..4838)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.26"
                     /tool="antismash"
     misc_feature    4826..4835
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     misc_feature    4827..4840
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.14"
                     /tool="antismash"
     gene            5001..6290
                     /locus_tag="A6A06_RS39415"
                     /old_locus_tag="A6A06_32180"
     CDS             5001..6290
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979781.1"
                     /locus_tag="A6A06_RS39415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32180"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_073768839.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-21, bitscore: 70.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTGQLSTFSAAAAAGPSPRTLTTTVPKEYVHKTALSEVLLTSW
                     KETDPDIHTVTAEWPRTHTFYSSRNRLHDPLLLVETIRQTIPLLSHLAYNVPFGHQLIW
                     DHFTYSLDPGTLRTETAPTEIELRIVCSDIKRRKDTLSALTMDVEATRNGIRLGTAVAR
                     FTSHAPAIYRRLRGEYGDAREAMARAVPLPPPVAPRRVARALYESVMLSPTDNRNRWQL
                     RTDTTHPVIFDHPVDHAPGMLLLEAAHQAVHAALHPLPVIPVGLSTVFARYAELDAPCW
                     IEATVLGPPEAGAESDAYPDSDAHPDSAAAAGAGTGAGAGAGAGAGAGAGAGAGAGAGA
                     GAGAGAGCGEEVGAVEFPEEFPPGAALWTDASGLVHTRVTAHQNGLELFTATVTARPLG
                     TDSDAGPGGESGTETGAEPSADSPGNPRTD"
     misc_feature    complement(6497..6514)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    complement(6837..6852)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.15"
                     /tool="antismash"
     gene            7101..7262
                     /locus_tag="A6A06_RS42440"
     CDS             7101..7262
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956719.1"
                     /locus_tag="A6A06_RS42440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_199931427.1"
                     /transl_table=11
                     /translation="MEQLIKKMAQDGVWREWVALNSPRGAGKDGCISATKSGCISATTA
                     APKSGCIS"
     gene            7589..8971
                     /locus_tag="A6A06_RS39420"
                     /old_locus_tag="A6A06_32185"
     CDS             7589..8971
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535674.1"
                     /locus_tag="A6A06_RS39420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32185"
                     /product="hypothetical protein"
                     /protein_id="WP_242441186.1"
                     /transl_table=11
                     /translation="MDGQPSRPGQPTADIVNRIRDIPIYRSSIERGHFPILDKPEIARG
                     FPDNWMTPVLADALAAGAAEYVLSTGTNHARMQIIRPPYFLLNSYYRLWSEHPDIAGTW
                     HEGCQRVSLTTVLATEHVARVNAKKHGRSAPAPVPALEDRRLDDRTFYVNLTLDPADWD
                     RDDVVRMLAEIRTAQQAHPHGRYHLDCSGYHLAHLVRKVTDWGLWDDFPRPASIVHAYE
                     YTPVNVRRYLHEHFDCPVIDLFGSTELGYLYYSDRHGRYHPYLDKMSVELIPVEPGSGI
                     FSMIVSSVRNPYMPLIRYRSGDCARTLDGTPDPEKISRFCGREKELLTSAHGRGPVAQG
                     DLDDCVSEASPRVFVHQLRVPGDRSGAELRYTTFDGTPLDASEAAALSAGVERLTGLPC
                     RVGHQARIPIGKSGKYAWLEREPERAAEPEQVRERERDPERAAEPEQVREREQERGPAG
                     EP"
     gene            9075..9623
                     /locus_tag="A6A06_RS39425"
                     /old_locus_tag="A6A06_32190"
     CDS             9075..9623
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979784.1"
                     /locus_tag="A6A06_RS39425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32190"
                     /product="hypothetical protein"
                     /protein_id="WP_073768840.1"
                     /transl_table=11
                     /translation="MTTQPQEPLQDAAAPEPHLTPDDLKALLGERLHAEVVQHFVAAGG
                     AEPAYVERQVTECLRYLYLVSRHREQLSGLFLPVEQDIDEIWHYLILQTREYREWCEQR
                     LPGRFFIEHRSIGYDDYQQEPGREQVLEEALRWIPLYVREFGPFDEGALPHWTIVRFLH
                     DQLSMSLRDIAALQPAGAP"
     gene            9856..11136
                     /locus_tag="A6A06_RS39430"
                     /old_locus_tag="A6A06_32195"
     CDS             9856..11136
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979785.1"
                     /locus_tag="A6A06_RS39430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_32195"
                     /product="MFS transporter"
                     /protein_id="WP_073768841.1"
                     /transl_table=11
                     /translation="MLALSQTLSGAGLAAGITVGALLAEEMLGSTGLSGLPAALFTGGA
                     ALGAVGIGRVCQRWGRRPGLTAGYATAGLGSLGVVIAAALGSVWLLFLSLLVYGAGTAT
                     TLMARYAGADLAAPARRGRAVSLVLFATTLGAVIGPNLVGVTGDVADGWGVPRLAGPFL
                     LAVVAYAAAAVVLGVFLRPDPLRLARARAAAAEAEAEAEKEAEAKAEAEAREGAAEAPP
                     ASPASPAVRADTRGIVTGTAVMTLTQLVMIAIMTMTPVHMRAHGHPTEAAGLVIALHVA
                     AMYLPSPLTGMLVDRIGRRRVAAASGLVLLAAGVLAALAPAHSVPALAAALVLLGIGWN
                     FGLVSGTAIVTDAVPLATRASTQGLVDVGIAVAGATGGMASGLVVATGGYSTLAMSGGI
                     VALGLVPLLVLRGRAGRGVRTEDGARP"
ORIGIN
        1 ccctcgtccc cgcccatcag gagcggcggc cgggcgccca gcaccaggcc ccacacgcgg
       61 tccacgtgct cccacgggga cagcccggcc gtggccccgc ggggcacgcc gatgcgggcc
      121 gcgaactccg cgccggtcac cacggagacc gccgtggcgg tgaccgcctc cgcggtcgtc
      181 cagccgacga agtccgcgcg ggcccgatcg acgagttcgg tgagcgacgg cagccacatc
      241 gcgtaccagg tgttgccgtc gtccgggaag tcccacgcga ggcgggcggc ggcccgtacg
      301 tcgggctcct cttcgagcag ccgcgcgagg gtgcgggtga aagtgacgac gcgtcgtaac
      361 tcgccccggg ccgccgtctt ggggcaccgg ctcgccgcca tgagggactc cagcgcgctc
      421 cgggtcagcg cgcagccgga gtccctgacc gcttcggcga gcgccgcctt ggacgcgaag
      481 tggaacgtca ggccgccgat cgtgacgtcc gccgcggtgc tgatacgggc catcgaggcg
      541 cccgcgtaac ccttctcggc gatctcgtcg gcggcggccc gcagcagggc ccggcgcgtc
      601 cgctcagctc ggtcctgtcg cgccattgcc ccgcctcggt gatcgccctc ggttgtcagc
      661 gccgtaccgg tgacatccaa ccgtccatgc tagagccggg tttgaccgcc cgcacggctc
      721 cggcggcagt tggcggagac ttgatcagta tgggaaccgg tatccgtccg gggtccgggg
      781 cccgtcccac gggggcgctc gttgacgcct gaaccgttcc ccggagggcg tcggccgggc
      841 ggccgccgaa gcggaatccc gccctgcgcc gggggcgcgg ctccggcggc ccgcgtcccc
      901 gcgtccggcc cgacgcgccg tcaggtacgc ggcccggccc cggcccggta tccgtacccc
      961 ctccggggcg cggccacccc gattcctccc ccgccgagcg cccgcggttc ggccggcgtt
     1021 caactgcccg cgaccggctg cgggtacgac gaaaaaagcc ccggacctcg tgggctcgca
     1081 aaaaaaacga tcgttcttta ttctttgctg gtcggcgccc gattcacggg atgaccacgg
     1141 gagggaacga acgcatgacg acagaaaccg cgcatccggc tacccgaacc cccgcacgcc
     1201 ccctgtccgc tcccctctct tccagccacc ccctcgcagt ccgcaccgcg cccgacaaat
     1261 ccggcgtacg ggatgtctcc ggcgcctccg ccgacaccca caccgccggt tccggaagga
     1321 ccgcccgccc cggccggtcc accgcccaca acggaggcac cggcagaacc tccgccgccg
     1381 gaaccaccgg ccccgcgggc cgaaccggaa ccgtccgccg gccgggccgg gccgacgccc
     1441 ccgccgcgcc cgcgccgtcc gccgccgcct ccggcgactc cgcttccacc gccgcgaccg
     1501 tgacatccgg cccgtccgag acgttcgacg ccttcgccga ggaccttttc ggccatctcc
     1561 cgcgtaccga ccagcgccgc tgggcccacg cgtatctgct ggcactgctg accaccgaag
     1621 gcaagaagtc cgtacgccgg ctcgccgcgg cggtctccga ctcgcccacc gcctcgcagt
     1681 cgctccacca gttcctgaac gccagcccct gggactggaa cccggtgcgc cgcgaaatcg
     1741 tccgccgcgc ggagcagccc gccccgcccc gggcgtggat cctcgccccg gcggtactgc
     1801 ccaagcgcgg cggccactcg gtgggcgtcc accgccgctt cgactccgcc gtcgggcgga
     1861 ccgtgagctg ccagctcgcc atgggcatgt tcctgccggt gggtgacgcc cccacggccg
     1921 ccgccgtctc cgcctccgcc gccgcgacct ctgccgtcac cgcagcctcg gccgcctcct
     1981 ccgtcgccgc ctctgtcgcc gcctccgccg ccggtgactg cggcggcagc gcggccgtgt
     2041 ccgtcgactg gcggctgttc ctgccgccgc agtgggccga ggaaaccgag ctgctgcgcc
     2101 gcaaccgcat ccccgcctcg gagacgagcc acccccgccc gatgtgggtg cacgccctcg
     2161 acctcgtgga agcccaggcc gcccgttcga cctgcccgcc ggcgccggtc gtggcggacc
     2221 tgagcgagta ctccgactcc gtacggctca tcgaggcgct cgcccggcag ggacgcgact
     2281 tcgtcatcgc cgtaccgccc gccacccccc tggccacccc ggccacgccc ggcatgcccg
     2341 gcgaccccgg cacccgcccg cgcctgctcc agtccgcgcc cacgcccgta cgtctgcccg
     2401 gctcccacca cacctaccgc gccttcgcgg aacccggccc ggccaacgcc cggccgcccc
     2461 gggtctggct caccaacatg gcccaccgcc gcatgggcca gctcgtcggc ctcgcccggc
     2521 tgcaccagct gcccgcggcg accctgcgca ccctcgcgac ggacttcgga ctgcacgact
     2581 tcgagggccg ctccttcccg ggctggcacc accacatgac gctggcctcc gccgcgcacg
     2641 cctaccgtgc cctggcggcg gagccggacg aggcgacgct gtccacggtg cggttcgtgt
     2701 cctgacgccg cgcaccgcgc cgccctaacg ggcgcgggcg cggtcctgaa ggtgcgaccg
     2761 gtaccagtcg gccgcctccg cgagccgtac gagggggcct gggccggggt cgcagcccgc
     2821 cgccgtccac acctcgtcgc tgcggtacca gtggtcgctg gcgagcaggg cgaactgacg
     2881 tgcgctcacc cggccggggg tggagccgag gcgcttcagg cactcgtccc aggggaggtc
     2941 ggggagggct tcggcgatgc cgttcggggg cgtggcgttc tgaggcgtgc cgttctgggg
     3001 cgttcccggc atgcctggca tgtcgttctg gggcgtggcg agcgtgccct tcggctgtgg
     3061 gaagacgccg tggccgatca gagtgtccat gaggtccccg ttgcgtacgg ggacggggtg
     3121 actcgcgtgg tgcacgccgc cggggatgcc gtccggcgcg agagccagcc gcgcgaagag
     3181 gcgggccagg tcgtcgaccg ccacggcgga gagcaggccg cgcccgccgt cccagcgtgc
     3241 cggtacgcgg cccagcagtt cggccagcgc cggtacgacc caccggtcgc ccgcgccgag
     3301 caccaggccc ggccgcagca ccgtgccgcc cgcctccagc accgggcgtt cggcggcgag
     3361 gcgggtgcgg ctggcggcgg agaccggtgc gggcgtgacc tcgtccacgc ccggacccgc
     3421 gtgcggaccg gccccgtaca cggccgcggt ggagagcatg acgatacggc cgacacccgc
     3481 gcgtacggct tcggcgacga gggcggcggt gccgcggacg ttgaccagct cgcagcgctc
     3541 ctcgtcccgg ccgatgtcgg aggcgaggtg gagcaggacg tcggcgcctt cacagacacc
     3601 gcggagcgag gcggggtcgg caaggtcggc ggcgacccat tcgacggggg cgccggccgg
     3661 ctgcggggct tccggcggct tgcgggcggt ggcgcggagg tggagggagt gggagtgggc
     3721 gtcggggtac gggcgggggt tcgggtcgag gtggcgcggc cccgtttccg tacccgaaac
     3781 cgaatcagga ttctggttga cgcccggatg tgaggtgctg ccctccggat gtgaggtgct
     3841 gccgtacggt tccgggtccg gccgcggccg gtcgccgggc gccgtcggcc ccgtggcggc
     3901 acgcagcagt tcccgcatca ccgcggagcc gatgaacccg gtcgccccgg tcaccaccac
     3961 ccgccggggc gccgtcgccg acacgcctcc tgacacgtca tccgccatgc tcgccgccat
     4021 gtcctctccc gctgcccacg tcacccgtgt cacccgtctt gatcgtcgcc accttcgcag
     4081 acggcggtcc gggttccgtc accggctcgg catacgtgca cgcatacatg tacgggcgtg
     4141 gcggcggata cgtgcgctcg ggcccgcgct cacgccgccg cccgccgccg ccgcgccgcg
     4201 aaggtgacct gcggcctgac gggcggcgcg gcgacgcccg ggagcaggta gcgccagagg
     4261 gagacgacgc gctggtgcag gtcggcgcgg ccggaggaga tctgcgagaa gagctgcgta
     4321 ccggtataag cgctgaccag gacccaggac agttcgtcca cgttcacctc ggggagcagt
     4381 tcgcccttct cgcgcgcggc gcgcagctgg ccgccgaact cctcggccca gtggcggtac
     4441 gcggagtcgt cctggacccc gaattcgccc tgctcgacag cgagcctgac gccggcccgc
     4501 agggtggggt tggactgcag ttcctccgcc aggaacaggg tgatgtcgac caggcgctgg
     4561 aggccgtcct ctccgggcgg cagttccaga ccgccgccct gctcgatcat cactgcctgg
     4621 gccagggctt ccttggacgg gaagtggaag tacatcccgc cctgggtcac accggcgcgc
     4681 ttcatgatct tgctgatact ggcgccgctg aacccgaact cgtcgaacac ctcggcggcc
     4741 gcccggagga tgacctcacg tgtcctgacc gcacgctcct gcatggacag caccagctcc
     4801 ctcgacaaac aaaacgaata ctatttattt tatgaaagcc gaggctctca cgcggtgacg
     4861 gctgaaccga ccacgaagcg aaccacgttg gccgccaaac ggcccgcgca ggaaagcgac
     4921 ggcctgcccc gggagccccg gcggaccaca acgggccgca acggaccgga cctgcagaca
     4981 ctcctcaggg ggatacccgc atgagcacca ccggccagct ttcgacgttc tccgcagctg
     5041 ctgccgccgg cccgtccccg cgcacgctga cgaccaccgt gcccaaggag tatgtacaca
     5101 agacagcact gtccgaagtc ctgctcacca gctggaagga gacggacccg gacatccaca
     5161 ccgtgaccgc ggaatggccg cgaacccata cgttctactc ctccagaaac cggctgcacg
     5221 acccccttct gctcgtagag acgatccggc agaccattcc gctgctctca cacctcgctt
     5281 acaacgtgcc gttcggacac caactgatct gggaccactt cacttattca ctcgaccccg
     5341 gcacgctgcg taccgagact gcccccaccg agatcgaact acgcatcgtc tgctccgaca
     5401 tcaaacgccg taaggacaca ctgagtgccc tgaccatgga cgtcgaggca acacggaacg
     5461 gcatccggct cggcaccgcg gtcgcgcgat tcaccagcca cgcaccggcc atctatcgcc
     5521 gtctgcgcgg tgaatacggc gatgcacggg aagcgatggc gcgcgcggtg ccgctgccgc
     5581 cgccggtcgc gccgcgacgg gtcgcccgcg ccctgtacga aagcgtgatg ctttctccca
     5641 cggacaaccg caaccgctgg cagctccgta cggacaccac ccacccggtc atcttcgacc
     5701 acccggtcga ccatgccccc ggcatgctcc tgctggaggc cgcacatcag gccgtccacg
     5761 cggcgctcca cccgctgccg gtcatacccg tcggcctgag caccgtcttc gcgcggtacg
     5821 cggaactgga cgcgccctgc tggatagagg ccacggtgct cggtccgccg gaggccgggg
     5881 cagagtcgga cgcatacccg gactcggacg cgcacccgga ctcggcggca gctgccggag
     5941 cggggacggg agctggagcg ggagcaggag cgggagcagg agcgggagca ggagcgggag
     6001 caggagcggg agcaggagcg ggagcaggag cgggagcagg ctgcggagag gaagtcgggg
     6061 ccgtggaatt cccggaggaa ttcccgcccg gggccgcgct gtggaccgac gcctccgggc
     6121 ttgtccatac gcgcgtcacc gcgcaccaga acggcctgga gctgttcacc gcgacggtga
     6181 cggcccgccc gctggggacc gattcggacg cggggcccgg cggggaatcc ggcacggaga
     6241 cgggcgcaga gccgagcgcg gattcacccg ggaacccgcg tacggattga cgcggggatt
     6301 gacgcggggg cgccgggcgg attgacgtac ggattgacgc gcgggcctgc acaggtccgc
     6361 acatccggat tcgcgggcgg agccacggac gaaatcgcgg ccggaaccgc ggagggaacc
     6421 ccggagggaa ccgcccggga ttcactcgcg gatccacgca aggagtgccc aaggagcgac
     6481 caagcagccg ctcaaggagc cgctcaaagg gacgctcaag gagccgcgta agaacccacg
     6541 cacggattcc cgtacggatt cccatacggg ttcaccggcg gatttcgcgc aggtccccgc
     6601 ccggaccgcg gcggccaccg cattccagca ggtgaagggg cgcggggccg ggccatccgg
     6661 cccgtcccgc ccgggtgtcc gggggcgctc gccgcgggaa cgcacattgg gtgccatccc
     6721 acaacgactt cgggagatcc accgggtgtt caccctgcgg ccaccggggc gcaaacgggt
     6781 cggcccacgg cgttccggcc accgttttta ttgtcatgca cacggcatac caatgctgtg
     6841 gtccagacca gagccgcctc acacgaatct cggccgcgag gggctacaac cagccacgac
     6901 acccctccga gctgtggcca tgacgcgcaa gatcaagcca aagtgtccgt tcgtgtcggt
     6961 gtttgagcca tacctgcatt gcaggccttt gacgatcact tggtctttct tttacgttct
     7021 agggagcggc acccgcgcga ctcgcgtggc tgatcatcgc cgttttccac catcccctgc
     7081 cctgcggagg ctcgacggac atggaacagc tgatcaagaa gatggcccag gacggcgtct
     7141 ggcgtgagtg ggtggccctg aactccccgc gcggagcggg caaggacggc tgcatcagcg
     7201 cgacgaagtc cggctgcatc agcgcgacca cggccgcacc caagtcggga tgtatttcct
     7261 gaccgggcgc tgagccttcc ctgcggggag tcggccggcg gaccgcacac ccccggccga
     7321 ctccccgcca cttgcattcc gcgtactccg tacgttcgac ggaatccgcg tgttgcgcgc
     7381 gttgcgcgcg tgaggcgcgt tccgcccgat ctgctctatc cgctcgatcc gtgcgttcct
     7441 cctgcttcac gtgttttcac atgctccaca cgttccccgt acaaggcaga cacaacgcag
     7501 gcataacgcc ggtacgacgc gcaggttctc gtacgccgcg aaaccgatca ctccccgtcc
     7561 gccagacaag acagacctga gggtgaccat ggatggccag ccgagccggc cgggacaacc
     7621 gaccgccgac atcgtgaacc ggataaggga cataccgatc taccgctcat cgatagagcg
     7681 ggggcacttc cccatactgg acaagcccga gatcgcccgc ggatttccgg acaactggat
     7741 gacgccggta ctggccgacg cactcgccgc cggcgcggcg gagtacgtgc tctccacagg
     7801 caccaaccac gcgcggatgc agatcatccg gcccccgtac ttcctgctca actcctacta
     7861 ccggctgtgg agcgagcacc cggacatcgc cggaacctgg cacgaaggct gccaacgcgt
     7921 ttcgctgacc acggtgttgg cgacggagca cgtggcgcgc gtcaacgcga agaagcacgg
     7981 ccggtccgcg cccgcgcccg taccggccct ggaggaccgg cggctcgacg accgcacctt
     8041 ctacgtcaac ctcacgctgg acccggcgga ttgggaccgc gacgacgtcg tacggatgct
     8101 cgccgagatc cgtacggccc agcaggcaca cccgcacggc cgctaccacc tcgactgctc
     8161 cggctaccac ctggcacacc tcgtgcggaa ggtgaccgac tggggcctgt gggacgactt
     8221 cccgcgcccg gcgagcatcg tgcacgccta cgagtacacg ccggtgaacg tacggcgcta
     8281 cctgcacgaa cacttcgact gcccggtcat cgacctgttc ggcagcaccg agctgggcta
     8341 cctctactac agcgaccggc acggccggta ccacccctac ctcgacaaga tgagcgtcga
     8401 gctgatcccg gtggagccgg gcagcggcat cttcagcatg atcgtgtcga gcgtccggaa
     8461 cccgtacatg ccgctgatcc gttaccgctc cggggactgc gcgcgcacgc tggacggcac
     8521 gcccgacccg gagaagatct cccggttctg cggccgcgag aaggaactcc tcacgtcggc
     8581 gcacggccgg ggtccggtcg cgcagggcga tctcgacgac tgcgtcagcg aggcttcgcc
     8641 ccgcgtgttc gtccaccagc tgcgcgtgcc cggcgaccgg agcggcgccg agctgcggta
     8701 cacgacgttc gacggcaccc ccctggacgc gtcggaagcg gcggccctga gcgcgggggt
     8761 ggagcggctg acgggccttc cgtgccgggt cgggcaccag gcgcggatcc cgatcgggaa
     8821 gtcggggaag tacgcgtggc tggagcggga accggagcgc gcggcggagc cggagcaggt
     8881 acgggagcgg gaacgggatc cggagcgcgc ggcggagccg gagcaggtac gggaacgcga
     8941 gcaggaacgg ggcccggcgg gcgagccgtg agtcgctgag cctgcgcgga agctcacagc
     9001 cccctcggca gacacccgaa ggcgcccgaa gacgcccgaa gacgcccgaa gcccctgaag
     9061 acggcaggag aaccatgacc acgcagccgc aggaaccgct ccaggacgcc gccgccccgg
     9121 agccgcacct caccccggac gacctcaagg ctctgctcgg cgagcgcctg cacgccgaag
     9181 tggtgcagca cttcgtggcc gcgggcggcg ccgagcccgc gtacgtggag cgccaggtca
     9241 ccgaatgcct gcgctacctc tacctcgtct cccgccaccg cgagcagctc agcggcctct
     9301 tcctgcccgt cgagcaggac atcgacgaga tctggcacta cctcatcctc cagacccgcg
     9361 agtaccggga gtggtgcgaa cagcggctgc cgggacgatt cttcatcgag caccggagca
     9421 tcggctacga cgactaccag caggaacccg gccgggagca ggtgcttgag gaagccctgc
     9481 gctggatacc gctgtacgtc cgggagttcg gccccttcga cgaaggcgcc ctgccgcact
     9541 ggacgatcgt acgcttcctg cacgatcagc tgagcatgtc gctgcgggac atcgcggcac
     9601 tccagcctgc cggcgcgccc tgagcgcgct ttcggggcag cccgagaacg aggaaacgta
     9661 cccatgagca ctcggcccgg cgaaccggct ccccatgccg acccccatgg cccatacggc
     9721 gattcacccg cgggcccgcc cggcgattca cccgcgccgc aagctccttc ggcacgccct
     9781 ggcgactcgc ccaagcccgt cgggcccccg gctgagccct cgggtgagcc cccggagcag
     9841 aagcggatcc tggccgtgct cgcgctgtcg cagacgctca gcggagcggg gctggccgcg
     9901 ggcatcacgg tgggcgcgct gctcgcggag gagatgctcg gttcgacggg gctgtccggc
     9961 ctgcccgcgg ccctgttcac cggcggggcc gcgctcggcg ccgtcgggat cgggcgggtc
    10021 tgccagcgct ggggccgacg gcccggcctc accgcggggt acgcgaccgc cggtctgggc
    10081 agcctcggcg tcgtcatcgc cgccgccctc ggctccgtat ggctgctgtt cctctccctc
    10141 ctcgtctacg gggcgggtac ggcgacgacc ctgatggccc ggtacgcggg cgccgacctg
    10201 gcagcgcccg cgcggcgggg gcgcgcggtg agcctcgtcc tgttcgccac cacgctcgga
    10261 gcggtgatcg gtccgaacct ggtcggggtc acgggcgatg tggcggacgg ctggggcgtt
    10321 ccgcgcctgg cgggaccgtt cctgctggcg gtggtcgcgt acgcggcggc ggccgtggtg
    10381 ctgggggtgt tcctgcgtcc ggacccgctg cggctggcgc gggcgcgggc cgcggcggcg
    10441 gaagcggaag cagaagcgga aaaggaagca gaagcgaaag cggaagcgga agcgcgggag
    10501 ggtgccgcgg aagctcctcc cgcctccccc gcctcccccg ccgtccgggc cgatacccgc
    10561 gggatcgtca ccggtaccgc ggtcatgacc ctcacccagc tcgtcatgat cgcgatcatg
    10621 acgatgacgc ccgtccacat gcgggcgcac ggccacccga ccgaggccgc cgggctggtc
    10681 atcgccctgc acgtggcggc gatgtatctg ccgtcgccgc tcaccgggat gctggtggac
    10741 cggatcgggc ggcggcgggt cgcggcggcc tccggcctcg tcctgctcgc cgccggtgtc
    10801 ctcgccgcgc tcgcgccggc gcactccgta ccggcgctgg ccgccgcact cgtcctcctg
    10861 ggcatcggct ggaacttcgg cctcgtcagc ggcaccgcca tcgtcacgga cgccgtgccg
    10921 ctggccacgc gcgcctcgac gcagggcctg gtcgacgtgg gtatcgccgt cgcgggtgcc
    10981 accggcggca tggcgtcggg cctggtcgtg gccaccggtg gttactccac gctggccatg
    11041 tccgggggga tcgtggcgct cggcctcgta ccgctgctgg tgctgcgcgg gcgtgcggga
    11101 cgtggcgtcc gcacggagga cggtgcccgc ccgtgaagat cttcgggaaa ctcaggaact
    11161 ccaggacctt cgcgggcctc acgcgcccgg acacggccgg ccacccggcc cactacacgg
    11221 catccaaccg accgcacagc ccgccgggcg tggagccggg ccggtccgtg ccccacaagg
    11281 caccgcgtac
//
