LOCUS       NZ_LWKZ01000027        10510 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold7, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000027
VERSION     NZ_LWKZ01000027.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 721891)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721891)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 215082
            Orig. end                         :: 225592
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10510
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10510
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10510
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5510](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5510
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            469..861
                     /locus_tag="A6A06_RS35800"
                     /old_locus_tag="A6A06_28620"
     CDS             469..861
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381428.1"
                     /locus_tag="A6A06_RS35800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28620"
                     /product="VOC family protein"
                     /protein_id="WP_073767592.1"
                     /transl_table=11
                     /translation="MVHVLSSRVLVRPSDPERSREFYGRALGLAIYREFGTGPERGTVY
                     FLGGGFLEVSGRSPEPPTATLQLWLQVDDAAAAHEELAARGVEILRPPVREPWGLVEMW
                     IADPDGHRIVLTEVPADHPLRYRPGI"
     gene            1221..1811
                     /locus_tag="A6A06_RS35805"
                     /old_locus_tag="A6A06_28625"
     CDS             1221..1811
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985732.1"
                     /locus_tag="A6A06_RS35805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28625"
                     /product="DUF5134 domain-containing protein"
                     /protein_id="WP_073767594.1"
                     /transl_table=11
                     /translation="MHGPPWVGWLLVLLCAGAGAYCLSSVRRGTAAQRREARGEALMGL
                     GMAAMAVPASAVAQPSWTPWAFAAVFGAAGLWALLLRHPHHAVGAAAMVYMALAMVSVP
                     GGGPEGGGHAAHTESMERAGAMGPGGLPAFTALLLAYYAVYVVVAGVRLAPAATPDAAD
                     VGTAVPVRAAWPEVVGACRVAMGIGMFAMLLAL"
     misc_feature    1822..1839
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            1970..2908
                     /locus_tag="A6A06_RS35810"
                     /old_locus_tag="A6A06_28630"
     CDS             1970..2908
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576210.1"
                     /locus_tag="A6A06_RS35810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28630"
                     /product="M56 family metallopeptidase"
                     /protein_id="WP_073767596.1"
                     /transl_table=11
                     /translation="MMVPLALMMLGALAAAMAPRLMARADWPDREPVLALWVWQCVVAA
                     VLLCCVLAMSLCGAAAWEAVRSHLFGHAPRVVVEAYALKAYGPWSGVLAAVLACGGAWT
                     AVMLTREVRGAHARRRQRRADLLLRSPLLPGEQPTADRLVVLEGDRPEAWWLHGSAAQL
                     VITTSALRRLKGRQLDALIAHEQGHARARHDVLLHCASALATGFPQVPVFAAFRDQVHR
                     LVELAADDVASRRFGRLTIALALVELNEDRGVFGPCPAQCAQVPHRVHRLLAPAPRFTP
                     GRRLRMTAVAALVPAVPLLVTFMPGLSALTG"
     gene            3140..3853
                     /locus_tag="A6A06_RS35815"
                     /old_locus_tag="A6A06_28635"
     CDS             3140..3853
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044451.1"
                     /locus_tag="A6A06_RS35815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28635"
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_242441124.1"
                     /transl_table=11
                     /translation="MPPMPPKPPKPPAPSLSPPRPHLFRHPLPSFLPCLLLSLLFAVLL
                     ALVSARWSPLLALDRDIATALHHTAVTAPGLTQANKVFSDWIWDPWTLRALLAVAFIAL
                     LRRGQWLLGCWVVVTAAVGTALQQVLKALVGRHRPVWPDPVDSAHYAAFPSGHAMTAAV
                     VGGLALWLLRLYGARRRWRWAAAALVAGSVAGVGFTRVYLGVHWATDVLGGWLLGAALV
                     AAASTAYARRYGTHR"
     gene            3885..4580
                     /locus_tag="A6A06_RS35820"
                     /old_locus_tag="A6A06_28640"
     CDS             3885..4580
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842944.1"
                     /locus_tag="A6A06_RS35820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28640"
                     /product="HAD family hydrolase"
                     /protein_id="WP_073767598.1"
                     /sec_met_domain="HAD_2 (E-value: 1.7e-14, bitscore: 49.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIKGVLFDFSGTLLRIESPESWLRAVLAETGTDVAEEDTVRLAA
                     ELSRAGALPGGSSPVRLPPGLAALWEDRDRSAGHHRELYTGLARQVALPDDALYDALYE
                     RHMTPAAWAPYPDAAAVLGELRRRGIRTGVVSNIGWDLRPVLRAHGLDRFVDSYVLSYE
                     HGIQKPDPRLFEIACEELGLAPTDVLMVGDDRTADGGATALGCGYLPVDHLPVTDRPDG
                     LRPALALAG"
     misc_feature    complement(4782..4796)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.26"
                     /tool="antismash"
     gene            5001..5510
                     /locus_tag="A6A06_RS35825"
     CDS             5001..5510
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950154.1"
                     /locus_tag="A6A06_RS35825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_073767600.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.8e-08,
                     bitscore: 29.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRISGRTLALGFASAALTVCSPATPAAADSPQATTVAADGTVITA
                     DDSEAGHVTTHKRGDGTEPPAELGNPSEWGVVKIQMTASAHSGRPDQEACVNASGGKWC
                     YGWYTDFNPGKYCYSNYLHETKWHRSSVKLGSGSRSSGSVKPGSASNAHLSAGLAYTCY
                     TYYSID"
     misc_feature    complement(5016..5029)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.01"
                     /tool="antismash"
     misc_feature    complement(5020..5035)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     misc_feature    5022..5024
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5720..6301
                     /locus_tag="A6A06_RS35830"
                     /old_locus_tag="A6A06_28645"
     CDS             5720..6301
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986788.1"
                     /locus_tag="A6A06_RS35830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28645"
                     /product="hypothetical protein"
                     /protein_id="WP_242441125.1"
                     /transl_table=11
                     /translation="MAGPVAGKWDNPFCAALSLVFSGGWSWACYAFLVGYFRRSKIEST
                     LLPSLGLAIGVVAYYLFKDMSPAVPAGLESGTSGEKLFSRILTWGIAAFALGAPVGLLG
                     NLARVPGVGGLPFRLIIPLVAFFETSMRLTAEADGQGPLVGMMWNVIRFAACAVALALV
                     GHTIWSWWHARRTRPSEADEHRARGGITSV"
     gene            complement(6485..7843)
                     /locus_tag="A6A06_RS35835"
                     /old_locus_tag="A6A06_28650"
     CDS             complement(6485..7843)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485563.1"
                     /locus_tag="A6A06_RS35835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28650"
                     /product="peptidase C39 family protein"
                     /protein_id="WP_073767602.1"
                     /transl_table=11
                     /translation="MSRPTPRRTVLAAALAVAAGTATPLAHAASPSAPSAAPKAKRLVD
                     YRAWHTAADWNQGVANGTRTVDGRRPGVVIDRPAGSADYRDPHTGATATWEYAVWTSPA
                     RTLKVPSTEAVVSWNAETPAGTWIAVELRGTYGDGVKTPWYVMGRWAAGDGDIRRTSVD
                     GQKDGRSSISTDTFAIDAPASGLRLTSYELRVTLYRKPGTTLTPTVWRLGLMGSDIPDR
                     FDVPAAKPGVATGRELVVPRYSQETHKGQYPEYDNGGEAWCSPTSSQMIIEYWGRKPTA
                     ADLAWVNPAYADPQVCHAARYTFDYQYEGCGNWPFNTAYAATYRDMEGVVTRLRTLSDA
                     ETLIAAGIPVITSQSFLAPELTGAGYGTAGHLMTVIGFTRTGDVVANDPNSPTNAAVRR
                     VYQRRQFEKIWLRTKRKNAEGKVVSGTGGICYVYFPTEVTAAQRHALSTVGIC"
     gene            8103..8729
                     /locus_tag="A6A06_RS35840"
                     /old_locus_tag="A6A06_28655"
     CDS             8103..8729
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985738.1"
                     /locus_tag="A6A06_RS35840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_28655"
                     /product="hypothetical protein"
                     /protein_id="WP_073767604.1"
                     /transl_table=11
                     /translation="MSQEPFRPFPPGPEHLGALAGRLRALPPSCGPVRLIAVDGHAGSG
                     KSTFARRLGAALGGAPVVRLDDIATHDELFDWTERFRAEVLEPLSRGESARYRVYDWER
                     REFAARRELAPAHAVIVEGVGAGRRAVRPYLACLLWMELTAGHSWERGRRRDGPELAAF
                     WDDWIPAERAHFAADPSRPHAGLLVRACAEGYEVLPGPGEVPGTP"
     misc_feature    8826..8839
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.07"
                     /tool="antismash"
     misc_feature    8836..8850
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    9505..9521
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
     misc_feature    complement(9541..9555)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    complement(9903..9914)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 cattccgaag tacacgtggt cgccgcggtg ttcgagctgg cagcaggcga cgatctgccc
       61 ggcgttctcg gcgagcagca gccggctctt cgcgtcgcgc accacggccg cgacacccgc
      121 ggggtcggtg cgctgcccct ccagcaggtc cgcctcggtg gtccagccgg cccggctagc
      181 gtcgccacgg taggcggact cgatcagctc gacgaccgcc gggacgtcgg cctcggtggc
      241 ggatctgtag gtcagctcga cggtcgtcat gggcggaggt cctctcggtg gcgtacggcg
      301 cggcggtacg ggcgccgcgc cggagcgccg cgcacggtac ggccgtaccg gaggctaaca
      361 agagcgaccg gacggccacc cgggccgtcc ggtgttccgc cacctcggcg gacggcttcc
      421 gaaaacgccc ccgtgatccg cggcgcccgg aggcgtaggg tccgcagcat ggtgcatgta
      481 ctgagcagca gggtgctggt gcggccgtcc gacccggagc ggtcgcggga gttctacggc
      541 cgcgcgctgg gcctggcgat ctaccgcgaa ttcggtacgg gcccggaacg cggaacggtc
      601 tacttcctcg gcggcggctt cctggaggtc tccggccgct cccccgagcc gcctacggcc
      661 acgctccaac tgtggcttca ggtggacgac gcggccgccg cgcacgagga gctggcggcc
      721 cggggagtgg agatcctgcg cccgccggtg cgggagccgt ggggcctggt cgagatgtgg
      781 atcgcggacc cggacggcca ccggatcgtc ctgaccgagg tcccggccga ccacccgctg
      841 cggtaccggc ccggcatctg accgccccgg cgtacggcct ccgcctcccg ggggcccgtg
      901 tccccggagt ccgtgtcctg ggagtccgcc tccccagggc ccgcctcccc ggccccgcgc
      961 cctgacgccc cgtactgccg gtctgccgtc ctgccgtccc ggcgcccttc tgtcccgccg
     1021 tcccgccgtc ccgccgtccc gccgcgcatg ctccccgtct gcgcccgtgc gctcccgatt
     1081 cagggtttcc cgcgggcgtc cgtgggccgt ccgcggggca cgatgccgct gtacggggct
     1141 gcacgacacc gggccgcgcg acaccgcgcc ggccacgccg cccgcgtgcg accgccgtac
     1201 gggctcgcgg ggaggacgcc gtgcacggac cgccttgggt cggatggctg ctggtgctgc
     1261 tgtgcgccgg agcaggggcg tactgcctgt cgagcgtgcg gcgcggtacg gccgcgcagc
     1321 ggcgggaagc gcgcggtgag gcgctcatgg ggctgggcat ggcggcgatg gcggtgcccg
     1381 cctccgccgt ggcgcagccg tcctggacgc catgggcgtt cgcggccgtc ttcggtgccg
     1441 cgggcctgtg ggcgctgctg ctgaggcatc cgcaccacgc ggtgggcgcg gcggcgatgg
     1501 tctacatggc cctcgccatg gtgtccgtcc ccggcggcgg gccggagggc ggcgggcacg
     1561 cggcgcacac ggaatccatg gagcgggcag gggcgatggg gccgggtggc ctgcccgcgt
     1621 tcacggcgct gctgctggcg tactacgccg tgtacgtcgt cgtcgccggt gtgcggctgg
     1681 caccggcagc caccccggat gcggcggacg tcggcacggc ggtcccggtg cgggccgcgt
     1741 ggccggaggt ggtgggggcg tgccgggtgg cgatgggcat cggcatgttc gcgatgctgc
     1801 tggccctgtg agcggatgcc gaagccctgt gagcggatgc ccaaggaggg cggatgccga
     1861 aggaggacgg cctctcctga gcgccgcccg cctcggatgc cggtggtata cgtcacttcc
     1921 ggcgcgagat cgtacctcgc ggtagccgcg cgctcatagg ctgacgccca tgatggtccc
     1981 tctcgcgctg atgatgctcg gcgcgctggc tgcggcgatg gcgccgcgcc tgatggcccg
     2041 cgccgactgg cccgaccgcg aaccggtgct cgccctgtgg gtgtggcagt gcgtggtcgc
     2101 cgccgtcctg ctgtgctgcg tcctcgcgat gtcgctctgc ggcgccgccg cgtgggaagc
     2161 cgtgcgcagc cacctcttcg gccacgcgcc gcgcgtcgtc gtcgaggcgt acgcgctcaa
     2221 ggcgtacggc ccctggtccg gagtgctggc cgcggtcctg gcgtgcggcg gcgcgtggac
     2281 ggccgtcatg ctcacccgcg aggtgcgcgg cgcccacgcc cggcgccggc agcggcgcgc
     2341 cgatctgctg ctgcgctccc cgctgctgcc cggcgagcag ccgacggccg accggctcgt
     2401 ggtcctggag ggcgatcgcc ccgaggcgtg gtggctgcac gggtccgccg cccaactggt
     2461 catcaccact tcggcgttgc gccgcctgaa gggccgtcag ctcgacgcgc tgatcgccca
     2521 cgagcagggg cacgcgcggg cccgccacga cgtcctgctg cactgcgcgt cggcgctcgc
     2581 gaccggcttc ccgcaggtgc cggtcttcgc cgcgttccgc gaccaggtgc accggctggt
     2641 cgaactggcc gccgacgacg tggcctcgcg ccgtttcgga cggctgacga tcgccctggc
     2701 cctggtcgaa ctcaacgagg accgcggggt gttcggaccg tgccccgccc agtgcgccca
     2761 ggtcccgcac cgggtgcacc ggctgctggc ccccgcgccg cgcttcactc ccggccgccg
     2821 gctgcggatg acggcggtcg cggcactggt cccggccgtc ccgctgctgg tgacgttcat
     2881 gccggggttg agcgcgctga cgggctgacg tgccggggcg tgctggggcg tgatggggcg
     2941 tactagggcg acgtccgagg cgctgaaccg cggtgctgac gcggcgcggt gccgacgcga
     3001 cgcgatgccg tcacggcgcg gcgctgaccc gcggagccac cggcgtacgc ggctccggcg
     3061 ggagcgcgcg gacgtgcgaa cgccccctcg tacggggact tcctggccct cccccacccc
     3121 cctccgcaaa gattccccca tgccccccat gccccccaag ccccccaagc cccccgcccc
     3181 atccctctca ccaccccgcc cccacctctt ccgccacccc ctcccctcct tcctcccctg
     3241 cctcctcctc tccctcctct tcgccgtact cctcgccctc gtgtccgccc gctggagccc
     3301 gctgctcgcg ctcgaccggg acatcgccac ggccctgcac cacacggccg tcacggcgcc
     3361 cggcctgaca caggccaaca aggtcttctc cgactggatc tgggacccgt ggaccttgcg
     3421 cgccctcctg gccgtcgcgt tcatcgcgct cctgcggcgc ggtcagtggc tgctcggctg
     3481 ctgggtggtg gtcacggcag cggtgggcac ggcactccag caggtgctga aagccctggt
     3541 cggccgtcat cgcccggtgt ggccggaccc cgtcgactcc gcgcactacg cggcttttcc
     3601 ttccggccac gcgatgacag cggcggtcgt gggcggcctg gcgctgtggc tgctacggct
     3661 gtacggagca cgtcggcggt ggcggtgggc cgccgcggcg ctggtcgccg ggtccgtggc
     3721 gggtgtcgga ttcacccgcg tctacctggg ggtccactgg gcgacggacg tgctcggcgg
     3781 ctggctgctg ggcgctgccc tggtggcggc cgcgtcgacc gcgtacgccc gccggtacgg
     3841 gacgcaccgg tgacggcggg ccgtcggcac gacaggatgg ccctatgacg atcaagggtg
     3901 tgctcttcga cttctccggg acgctgctgc gcatcgaatc gccggagagc tggctgcggg
     3961 cggtgctggc cgagaccggg acggacgtcg cggaggagga caccgtccgc ctcgcggcgg
     4021 agctgagccg cgccggcgcg ctgcccggcg gttcctcgcc ggtgcgcctg ccgcccggtc
     4081 tggccgctct ctgggaggac cgggaccgca gcgccggtca tcaccgcgag ctgtacaccg
     4141 ggctggcccg gcaggtggcc ctgcccgacg acgcgttgta cgacgcgctg tacgagcgcc
     4201 acatgacgcc cgccgcgtgg gcgccgtacc cggacgccgc cgctgtcctg ggcgaactgc
     4261 ggcggcgcgg catccgtacc ggcgtggtca gcaacatcgg ctgggacctg cgcccggtgc
     4321 tccgcgcgca cggtctcgac cgcttcgtcg acagctacgt cctgtcgtac gagcacggca
     4381 tccagaagcc ggacccgcgg ctgttcgaga tcgcgtgcga ggagctgggg ctggcgccca
     4441 cggacgtcct gatggtcggc gacgaccgga cggcggacgg cggcgcgacg gcactcggct
     4501 gcggctacct gcccgtcgac cacctcccgg tgaccgaccg cccggacggc ctccgccccg
     4561 ccctcgcact ggccggctga ccacccacac gaccaccggc cctacggccg caacacctcc
     4621 gtgtgaacgg ccagcttgaa ctgggcgtgc gcccggccgg cgtccccgga tgcacgaaca
     4681 tctgccacgg gtacgtcttg tactggccgc ttgccttctt gcatacctgg gctctgcagg
     4741 cgcgaccgca aagaaaagat cgagccgatc ggttcagcac ctattggcag aagatggcgg
     4801 catcgagatc gctgtagtcg ggcggcgaaa taggacagca cgcatcaaga tgcacgacgg
     4861 aacgcgaact tcccattcgg cccgcaagtc gggcgagatg cttttcgggc agccccttgt
     4921 cgagactgtt gactcctacc atttcctcgt tgaaaatctc cgcggaaccc ccacaggagg
     4981 ggttgaggag ggggaatgtc gtgaggatat ccggcagaac gttagctctc ggctttgcga
     5041 gcgcggctct cactgtatgc agccctgcta cgccggccgc cgcagacagc cctcaggcta
     5101 caaccgttgc cgcggacggc actgtcatca ctgcggatga ttccgaggcc ggtcacgtca
     5161 ccacccataa gcgtggcgac ggcacggaac cgcccgccga gctgggcaat ccctccgaat
     5221 ggggcgtggt gaagatccag atgacggctt cagctcactc cggcaggccg gaccaggagg
     5281 cctgcgtcaa cgccagcggt ggaaagtggt gttatggctg gtacaccgat ttcaaccccg
     5341 gcaagtactg ctactcgaac tatttgcatg agacgaagtg gcacagatcc tcggtgaagc
     5401 tcggcagcgg ctcacgctcc agcggttcgg tgaagcccgg cagcgcctcc aatgcccacc
     5461 tctcagcggg tttggcctac acctgctaca cctactacag catcgattag gctctgatcc
     5521 ccgcatcaag cggtgccccc tcggcagaca gggcaggttg gcgcaccgct cgggggccgt
     5581 tcggtcgctc gtgcggtcct ggagtggcgc cgcctccggc tctggctcaa tcacggaaca
     5641 ggcaagggaa atcatacatt taccacgcac tcgcgtcgtg ccgcttgcag tgtctctagg
     5701 ggcgggtttg ctactcggct tggcgggccc cgtcgccgga aagtgggaca atcccttttg
     5761 cgccgccctg agtcttgttt tttccggcgg gtggtcgtgg gcttgctatg cgttcttggt
     5821 gggctatttc cgccggtcca aaatcgagtc aacgctgctg ccgtctttgg ggttggctat
     5881 cggggtggtc gcgtactacc tgttcaagga catgagcccc gccgtcccgg ccgggctgga
     5941 atccggcacc tccggcgaga agctgttctc caggattctc acgtggggaa ttgcagcttt
     6001 cgccctcggg gcacccgtgg ggctcctggg caacctggcg cgagtgccgg gtgtcggcgg
     6061 tctccccttc cggctgatca ttccgctcgt agcctttttc gagacctcca tgcggctgac
     6121 cgcagaagcg gacgggcagg gcccgctggt cggaatgatg tggaatgtga ttcggttcgc
     6181 cgcctgcgct gttgcgttgg ccctggtggg tcacaccatc tggagctggt ggcacgctcg
     6241 gcgcactcgc ccctcggagg ctgacgagca ccgcgccaga ggcggcatca cgtctgtgtg
     6301 accttctgga gtccggcccc ccgccatcgc gcgaccctgc gtccgtgaac ccctcgcccc
     6361 ggcggccccg ccggttccat tgcgccacag gttccggtcg gccggtagac ggcgggcgga
     6421 atttggggcg gtgctcggga accgggcggg gggagcgagg agggagtgag gggggagcgg
     6481 cggttcagca gatgcctact gtggacaggg cgtggcgttg tgcggcggtg acctcggtgg
     6541 ggaagtagac gtagcagatg ccgccggtgc cgctgacgac tttgccttcg gcgttcttgc
     6601 gtttggtgcg gagccagatc ttctcgaact ggcggcgctg gtagacgcgg cggaccgcgg
     6661 cgttggtcgg cgagttgggg tcgttggcga cgacgtcgcc ggtgcgggtg aagccgatga
     6721 cggtcatcag gtggccggcc gtgccgtagc ccgcgccggt cagttcgggg gccaggaagg
     6781 actgcgaggt gatcaccggg atgcccgccg cgatcagggt ctcggcgtcg gagagggtgc
     6841 gcagccgggt gacgacgccc tccatgtccc ggtacgtggc cgcgtaggcg gtgttgaagg
     6901 gccagttgcc gcagccctcg tactggtagt cgaaggtgta gcgggcggcg tggcagacct
     6961 gggggtcggc gtaggcgggg ttgacccagg ccaggtcggc ggcggtgggt ttgcggcccc
     7021 agtactcgat gatcatctgg gaggaggtgg ggctgcacca cgcctcgccc ccgttgtcgt
     7081 actccgggta ctggcccttg tgggtctcct gcgagtaccg cggcacgacc agttcgcggc
     7141 cggtcgcgac gcccggcttg gcggccggca cgtcgaagcg gtcggggatg tcggagccca
     7201 tcaggccgag gcgccacacc gtgggcgtca gggtggtgcc gggcttccgg tagagcgtga
     7261 cgcgcagctc gtacgaggtc agccgcaggc cgctcgccgg ggcgtcgatg gcgaaggtgt
     7321 cggtggagat gctgctcctg ccgtccttct ggccgtcgac ggaggtacgg cggatgtcgc
     7381 cgtccccggc ggcccagcgg cccatcacgt accacggggt cttcaccccg tcgccgtacg
     7441 tgccgcgcag ctcgacggcg atccaggtgc cggccggcgt ctcggcgttc caggagacga
     7501 cggcttccgt ggacgggacc ttgagggtgc gggccgggga ggtccagacg gcgtactccc
     7561 aggtggcggt cgcaccggtg tgtgggtcgc ggtagtcggc ggagccggcc gggcggtcga
     7621 tcaccacgcc ggggcggcgc ccgtcgacgg tgcgggtgcc gttcgcgacg ccctggttcc
     7681 agtcggctgc ggtgtgccag gctcggtagt ccacgagacg cttcgccttc ggtgcggcgg
     7741 acggtgcgga aggggaggcg gcgtgcgcca ggggggtggc cgtgcccgcc gcgacggcga
     7801 gggcggcggc caggacggta cgccggggcg tgggtctgct catggtggag tcccccagtc
     7861 ggatgcgatg gtttgagcca ggagaacggt ggtgctcaac tattgccctt gcttgcgccc
     7921 ttcggccagc cattcgtcgc gcgtcgcggc agggatcacc ggggtgcggc ggccggggcc
     7981 cgtacgcggg cgcgggcgtt cccgggaacc ggcagcccgc aggccccgcg gacccgctgg
     8041 gccgcacgtc ccgtaccggt aatcttgcgg cgtcccaccg gtcacaccag acaggcagca
     8101 gcgtgagtca agagccgttc cggccgttcc cgcccggccc ggagcacctg ggcgcgctcg
     8161 ccggccggct gcgcgcgctg cccccgtcct gcggccctgt ccggctgatc gcggtggacg
     8221 gccacgccgg atccggcaag agcaccttcg cgcggcgcct gggcgcggcg ctcggcggcg
     8281 cgccggtcgt ccgcctggac gacatcgcga cgcacgatga actgttcgac tggacggagc
     8341 ggttccgcgc cgaggtcctg gaaccgctgt cccgcgggga gagcgcccgg taccgggtgt
     8401 acgactggga gcgcagggag ttcgccgcgc gccgggagct ggcaccggcg cacgccgtca
     8461 tcgtggaggg cgtcggtgcg gggcgccgcg ccgtgcgccc gtacctggcg tgcctgttgt
     8521 ggatggaact gacggcaggt cactcctggg aacgggggcg gcggcgggac ggaccggagc
     8581 tcgccgcgtt ctgggatgac tggatcccgg cggagcgggc ccatttcgcg gcggacccct
     8641 cgcgtccgca cgccggtctg ctggtacggg cgtgcgccga gggctacgag gtgctcccgg
     8701 ggcccggtga ggtgcccggt accccttgaa ccgtcaccga acgtgacgaa ccacgggcgg
     8761 tacgcagatc gtcgccctac gcgtaccgcc gactgcctca actcggcttg acctgggggg
     8821 cgtacaggac ttacgttctc aatgtgcggt tctgacggac cgcccgcaga cgcgaagccc
     8881 ccggttgttc ccccgtgatc gggggcttcg ttctgtgccg ggcgctcttc gcgcgccgcg
     8941 caccgtccgc ctcctcaccc tatgtcaccg cccccttcgc gccgcggagc ctcgtcttcc
     9001 cccgtaccgc cctcttcggc ctgcaccgat accgcaggta cgatgcgagt ctgtgccacc
     9061 gaaggacgca aacgaagggc ccggggggac cggcaactgc cgccccgggt aaggcggttc
     9121 gatcgaacac gctgcggcct ggcactcgcc cggcggcaca ccacggggga cgttttgtgg
     9181 gggacgtgat ggatttcggc acgcagggca cacacgcccc ggccgaactc gcctggcttc
     9241 gcgcggtgga cgcctacaca gtaggcgctt acccgcaggc cgaggaggag ttccgggccg
     9301 cggtgcggat ggatccgacg atggccgacg gctggctcgg cctgcacgcg ctgcgtgcgg
     9361 acacctcggc cgcgctgctg cacatgcacc ggcaccgcga ccgtttcggg gagcagcggg
     9421 cccggcaccg acgcacactc aattcctggt actggctcgg ctggtgggtg cagccggtac
     9481 tggagaccgg ccgcgacctg ctgctggcgc acgcctcgca ctggctcgac ggccgccatg
     9541 tggccgagct ggaccaggcg ttggcgggct gcccgccggt ggacaccgac ccgcaggtgc
     9601 gcttcctgca cgcctgccgc tcctatctcg tcaaggactg ggaccagctc gtacggcaca
     9661 ccgaacccct gctcgacgac ccgctgctcg gcatcgaggc cgggctgttc ggcggcatgg
     9721 cccgggtgcg ccaggagatg tacgggcagg ccgagccgct gctgtcggcc gccctgatgc
     9781 gctgccgcag cgagcagccg cagcgcaagg agctgcggta ctggctcgcc cgcgcccacg
     9841 agggcaccgg ccgcagcgcg gccgcgctgc cgctgtaccg ctcggtgcac cgggtcgacc
     9901 cggcgttcat ggacaccgcg gcgcggctcg cggccatagc cgagggggac ggcctggagg
     9961 agggcaccga cctcgcggcc gtctccgccg gtttcggcca ggaggcgggc ggggatctcg
    10021 acccgctctc cccgctcgat ccggtggacg ggcgggagct gctggccacg accgagccgg
    10081 agggcccgga cggtgacctg gcgggcggtt cgccggggga cgccggcccg gccgtacggg
    10141 agaaggccgc gtccggcggc ccgcgcgggt ccggtcagct gcccgccggt ccgtccgatc
    10201 cggtgctgct ggagaaggcg ctcgccgagc tggagcggat ggtcgggctg gagccggtga
    10261 agcgtcaggt cagagcgctg tcggcgcagt tgcgcatggc acggttgcgg gccggccagg
    10321 gccttcctgt gcagccgccg aaacgacact tcgtcttctc cgggccctcc ggcaccggca
    10381 agaccaccgt ggcccggata ctcggccggg tgttctacgc gctcggactc ctcggcggtg
    10441 accacctcgt cgaggcgcaa cgggccgacc tggtcggcga gttcctgggg cagaccgccg
    10501 tgaaggcgaa
//
