LOCUS       NZ_LWKZ01000024        11350 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold4, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000024
VERSION     NZ_LWKZ01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1153214)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153214)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 895021
            Orig. end                         :: 906371
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11350
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11350
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11350
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6350](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6350
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(111..788)
                     /locus_tag="A6A06_RS27125"
                     /old_locus_tag="A6A06_20195"
     CDS             complement(111..788)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="A6A06_RS27125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20195"
                     /product="AIM24 family protein"
                     /protein_id="WP_073764164.1"
                     /transl_table=11
                     /translation="MHSDLFASENVVTPASVPGMTLQNAKSIKYAVDGECLARQGSMVA
                     YRGDLQFEKQGQGVGKFLKRAVTGEGLALMAVRGRGEVWFAHEAANCFIVDLAPGDGIT
                     INGRNVLCFDPSLSYEISTVKGAGMTGGGLFNSVFSGQGRLGVVCEGNPLVIPVSPQAP
                     VFVDTDAVVGWTASLKTSLHRSQSLGSMLRGGSGEAVQLRLDGEGFVIVRPSELRPERA
                     TGN"
     gene            875..1648
                     /locus_tag="A6A06_RS27130"
                     /old_locus_tag="A6A06_20200"
     CDS             875..1648
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984734.1"
                     /locus_tag="A6A06_RS27130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20200"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_073764700.1"
                     /transl_table=11
                     /translation="MTSTDLAGTGDPTDAAAAAAAATPSAETGRDAAPQYVLPLVVRIE
                     RAEPPARTDALETAARAVLTLLADERSAGDGEWAAAVRDWQDARIRKVVRRARGAEWRR
                     AEALPGITVHGGSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPHEPPAPAAGTPVLWL
                     NPELDMSAGKSMAQAGHGAHLAWWDLSEAERTAWREAGFPLSVRNAPARRWTGLTHGGL
                     PVVRDAGFTEIAPNSCTVVSDHPALRARLGAGADA"
     gene            complement(1757..2122)
                     /locus_tag="A6A06_RS27135"
                     /old_locus_tag="A6A06_20205"
     CDS             complement(1757..2122)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982169.1"
                     /locus_tag="A6A06_RS27135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20205"
                     /product="darcynin family protein"
                     /protein_id="WP_073764166.1"
                     /transl_table=11
                     /translation="MSAEETAPPVTAFMLIKTTPEWLSMTPHERVSAMDLEVMSVVKAK
                     TTGIRSRFYDTEFYSTRVTDVWVWEADDHNAYQLLIDALRETPFWDRYFEIVDILVGAE
                     NGYARTYNVDAVTTVST"
     gene            2271..3215
                     /locus_tag="A6A06_RS27140"
                     /old_locus_tag="A6A06_20210"
     CDS             2271..3215
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1125: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548163.1"
                     /locus_tag="A6A06_RS27140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20210"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_199931175.1"
                     /transl_table=11
                     /translation="MNSPEPVIDAHLAVALDALLAEQSVTRAAARLHTSPAAMSRTLGR
                     LRRVLQDPLLVRAGQTMVPTPRAQALRDEAATVVRRLGALLSPGAGVDPAALHSTFTLQ
                     AADLVGAALAPGLLQLAGQEAPGVSLRFRAEDLEAGPALRDGRIDLEVGSIDHVDPETR
                     VEELLALRMVAAVRPDHPLTGRTLTPARLAAAEHVVVSRRGRFTGPLDTALAELDLHRR
                     VTVVLPSHLAAMALAARSDVVCLIPAALPGAAPSPLTDAATTFGLHLLDIPLPLPPMTL
                     GMAWHPRHAADGAHHWLRDAVRRTLRAAGSPAT"
     gene            complement(3294..4004)
                     /locus_tag="A6A06_RS27145"
                     /old_locus_tag="A6A06_20215"
     CDS             complement(3294..4004)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /locus_tag="A6A06_RS27145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20215"
                     /product="nuclease-related domain-containing protein"
                     /protein_id="WP_073764170.1"
                     /transl_table=11
                     /translation="MSDIRPEPGAEEGKQETRYASDPDRLFLPPDDDLAPNRPGEALRV
                     RLAEQPRSRAALWCARLLGRRPPEDAWHRQLLGEQAVGTALDQLTAGGWHVIHSVPVPP
                     AAAIGHLLIGPGGVLCVATHHVRGARVRVAEEAVAISRGHQSRPYVREIRQDARRASYV
                     LTRGCRFRVEVRPALAFVGAVDVELDAALRDDVRVIRDEREVPLLGRLGGVLAPDRVER
                     VYTVARNRRTWLRA"
     gene            4131..4838
                     /locus_tag="A6A06_RS27150"
                     /old_locus_tag="A6A06_20220"
     CDS             4131..4838
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="A6A06_RS27150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20220"
                     /product="LysE family translocator"
                     /protein_id="WP_073764172.1"
                     /transl_table=11
                     /translation="MLSSLLGFTAVAVLLTVSPGPDFAVVLRTSLGSGRRSALCSALGI
                     AAGCVVWGLAGAAGLTAMLSASQTAYDALRVAGALYLMWLGFQALRSARRSSKAARDAA
                     ASATAAAGAPLPDPAAGHAPVAGSGPAVDQQSPLQAFRTGLLTNVLNPKVGVVYMSLLP
                     QFIPHGAPVVTTTLLLVAVHVALGFVWLGGIALAVHRARAFFQRPRVRRRMDQATGGVL
                     LALGAAVGVETAR"
     gene            5001..6350
                     /locus_tag="A6A06_RS27155"
                     /old_locus_tag="A6A06_20225"
     CDS             5001..6350
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175675.1"
                     /locus_tag="A6A06_RS27155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20225"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_073764702.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-95, bitscore: 315.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEHLGTGIGWRPEIADETAELPGVDWVEVVAENICPGHLPPALQA
                     LRERGTTVVPHGVSLGLGGAERPDPGRLAALAERAEALRAPLVTEHIAFVRAGGPLTAS
                     PAMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALVNWPGEEMTEGQFLA
                     QLVERTGVRLLIDVANLHTNHVNRNEDPATALDELPVEAIAYVHVAGGIERDGVWHDTH
                     AHPVTRPVLDVLAALCARVTPPGVLLERDEDFPASGELAAELDAIRTVLKEAPPVPAPR
                     RTGGGPAATTTTISTATAATATATTATVTPTTAARQRLAVGQTALLSALVAGTPAPEGF
                     DRRRLLVQSRALAAKRASVVAKIAPELPDILGEDYRPGFLAYARHRPLHNGYRQDAMDF
                     AVHLLADGRPTDPDARRRLTQWWRERAGSKPPGGPVNRLARAVRHALHRR"
     gene            6356..7399
                     /locus_tag="A6A06_RS27160"
                     /old_locus_tag="A6A06_20230"
     CDS             6356..7399
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="A6A06_RS27160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20230"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_199931176.1"
                     /transl_table=11
                     /translation="MPALFVTLAVAASSLLLVVRTVHVVLATPKLKIFGPPDPELWEVA
                     FLSGGPARVADAAIAGMHQDGRLAVGGPGVVSVRQAVAHDPVETAVLDSLARTPDGSLG
                     TLRRHVMRAPAVQAIGDRLAKRGQMRHPRTGRPWRRLAGWQAGICAAGFLLVLLFTLIG
                     AIDSRGSGVPFVVRILPAAFVGIVVGGVCRRVLGRRVTRAGVSAVRKFRTANAARLVSD
                     PTQSAALIVALGSTALLVDDVLRDHLVEAQGSAAGASAGAYSGTSSTASSSSSNASCGW
                     NVPDDGWHTSWCGTSCGGGSGSSCGSSSCGGGSSSCSGGSSSCGSSSGSSCSSSSSSCG
                     GGGCGGS"
     misc_feature    7555..7575
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.7"
                     /tool="antismash"
     gene            7804..9165
                     /locus_tag="A6A06_RS27165"
                     /old_locus_tag="A6A06_20235"
     CDS             7804..9165
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6A06_RS27165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6A06_20235"
                     /product="hypothetical protein"
                     /protein_id="WP_073764176.1"
                     /transl_table=11
                     /translation="MPVEEFPIQQTGGSLVPHSVAVDPQGWLSVTTDNSALVQISRRDP
                     TAQTVHRDALPEFAAGILKDPTGRKVVWLASPTGPQDSDPIHEFDVEARRVINKHGLQD
                     GTRAQAITGVRVKTGSGPSDASKGYVVFAEPTHTSVGYVEVGKDGANHHPLPTGTDEFD
                     TWLWSVAATLDTTRTKCTYWATGQKHDEPERKTNGLYTYTPADDDTWQRIPLPNGAEQV
                     PIHVIAGVLREDERRDPYLWISARNPHQILRYDIRNKSWASSPTLTGEPRQLAFGPDGN
                     IWVAGTDQIHCFEKDAALRSLPVPALPSGSEAHGICVDPSDQVLWYTNRKTNTIGKYPL
                     PDGSRATTGKTQVLAQPQKETCPGAVAEVPMVAEFVALGEPTPEIPLTCRIISEEATFT
                     DGSRERVIATDPVGQVAFPAVLAGKVEEDVFLKMSWGDNEPSTTVILRVRNDEE"
     gene            9457..10257
                     /locus_tag="A6A06_RS27170"
                     /old_locus_tag="A6A06_20240"
     CDS             9457..10257
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643868.1"
                     /locus_tag="A6A06_RS27170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_20240"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_073764178.1"
                     /transl_table=11
                     /translation="MRSVTGTGLVVAALAATLAALLFPIWSFADRPGAGGDVNTQTVAT
                     SFGPLSALDRDFVNRVRLAGLWELPAGQQAQQRATVPAVREAGDHLVDGHASLDRHVRE
                     VAAQLGLALPDRPNAQQQQWLSELSAARGAAYDRKFANILRLAHGKVFTVVAQVRATTR
                     NSLVRGLADDANRTVLDHISMLERTGFVDFDAIAQDAATASPAPSPSAPPPSLDAAPGT
                     PPVPVTPSPTYSLPPPATPPATATGGATKAPESTEPPGGRRQHP"
     misc_feature    10539..10552
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 21.17"
                     /tool="antismash"
     misc_feature    complement(10540..10553)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.67"
                     /tool="antismash"
ORIGIN
        1 cgtagcgccg cccgaccccg tccagtctca tcggctcccc gccctggtcg tccccgttgt
       61 tcctgctggc cctgctgtcc ctactgcccc tgctgtcgcc ccggtagccg tcagttcccg
      121 gtcgcccgct cgggacgcag ctcgctgggc cgtacgatca cgaagccctc gccgtccagc
      181 cgcagttgca cggcctcgcc ggagccgccg cgcagcatcg agccgaggct ctgcgaacgg
      241 tgcagcgagg tcttcagcga agcggtccac ccgaccacgg cgtcggtgtc gacgaagacc
      301 ggggcctgcg gcgacacggg gatgacgagg gggttgccct cgcacaccac gcccagccgc
      361 ccctggccgc tgaagacgga gttgaacagg ccgccgccgg tcatccccgc gcccttgacg
      421 gtggagatct cgtaggacag ggacgggtcg aagcacagca cgttgcggcc gttgatcgtg
      481 atgccgtcgc cgggggcgag gtcgacgatg aagcagttgg cggcctcgtg cgcgaaccag
      541 acctcgccgc ggccgcgcac ggccatcagg gccaggccct caccggtgac ggcccgcttg
      601 aggaacttgc cgacgccctg cccctgcttc tcgaactgca ggtcgccgcg gtaggcgacc
      661 atcgagccct ggcgggcgag gcactcgccg tccacggcgt acttgatcga cttggcgttc
      721 tgcagggtca tgccgggcac ggaggccggc gtgaccacgt tctcggaggc gaagaggtcg
      781 ctgtgcatag ggaggataat cacccggaat ggttcgctcc gccaaggtgg tggccagggc
      841 agcggcgttc cggacggctg gcacactgga ccgcgtgacc agcaccgacc tcgccgggac
      901 cggcgacccg accgacgccg cggcggccgc ggccgccgcg acaccttccg cggagaccgg
      961 ccgcgacgcc gccccgcagt acgtcctccc cctcgtcgta cggatcgagc gggccgaacc
     1021 gcccgcccgc acggacgcgc tggagaccgc ggcgcgggcc gtgctgaccc tgctcgcgga
     1081 cgagcggtcg gcgggcgacg gcgagtgggc ggcggccgta cgcgactggc aggacgcccg
     1141 tatccgcaag gtcgtgcgcc gggcccgcgg cgcggagtgg cggcgcgccg aggcgctgcc
     1201 ggggatcacg gtgcacggcg gcagcgcgga ggtccgcgtc ttcccgccgg tcccgctgga
     1261 cggctggccc aaggacctgg cccgcctcca ggtctccggc accgacctcg acgatccgca
     1321 cgagcccccc gcccccgccg cgggcacccc ggtgctgtgg ctcaatcccg aactggacat
     1381 gtcggcgggc aagtcgatgg cccaggccgg gcacggcgcg cacctggcgt ggtgggacct
     1441 gtccgaggcc gagcgcacgg cctggcgcga ggccggcttc cccctgtcgg tacggaacgc
     1501 gccggcccgc cggtggaccg gactgacgca cggcggtctg cccgtggtgc gggacgccgg
     1561 gttcacggag atcgctccga attcgtgcac ggtcgtctcc gaccacccgg cgctgcgcgc
     1621 ccggctcgga gcgggtgcgg acgcctgagg cgggggcacc tgaggcgggg gcgcctgagg
     1681 cgggggcgcg caggccgaag ccctccggcc gtgtggggcg ggagggcagc ggggttcacg
     1741 tgggccgggc ggtgtatcag gtgctgacgg tggtgacggc gtcgacgttg taggtgcggg
     1801 cgtagccgtt ctcggcgccg acgaggatgt cgacgatctc gaagtagcgg tcccagaacg
     1861 gggtttcgcg cagcgcgtcg atgaggagct ggtaggcgtt gtggtcgtcg gcttcccaga
     1921 cccagacgtc ggtgacgcgc gtggagtaga actccgtgtc gtagaaacgc gaccggatgc
     1981 cggtggtctt ggccttgacc accgacatga cctccaggtc catggcgctc acccgctcgt
     2041 ggggagtcat ggagagccac tcgggcgtgg tcttgatgag catgaaggcg gtgacgggcg
     2101 gcgcggtttc ctcagcggac atgatgagtg cctccgaagt ggcaggtgaa gaggatcgtg
     2161 aagacccgtg ggacgtaccg gaccggtacg gctccatcct ggggagaagc acggtgttgc
     2221 cacaatggga acttcagcaa cggatcattg cctgaaatgg aatcacgctg gtgaacagtc
     2281 cagagccggt catcgacgcc catctcgccg tcgcgttgga cgctctgctg gcagagcaga
     2341 gcgtcacccg cgccgccgcc cgcctgcaca cctcccccgc cgccatgagc cgcacgctcg
     2401 gccgcctccg ccgcgtcctc caggaccccc ttctggtccg ggccggacag accatggtcc
     2461 ccaccccgcg cgcccaggcc ctgcgcgacg aagccgccac cgtggtgcgc cgcctcggag
     2521 cgctgctctc tcccggcgcc ggtgtcgacc ccgccgccct gcacagcacc ttcacccttc
     2581 aggccgccga cctggtcggc gcggcgctgg cgcccggact gctgcaactg gccgggcagg
     2641 aggcgcccgg ggtttcgctg cggttccggg ccgaagacct ggaggccgga ccggccctcc
     2701 gggacggccg gatcgacctg gaggtcggat ccatcgacca cgtcgacccc gaaacccggg
     2761 tcgaggaact gctcgccctc cggatggtgg cggccgtccg gcccgaccac cccctcaccg
     2821 gcagaacgct gacgccggcc cggctcgccg ccgccgaaca cgtcgtggtc agccgccgcg
     2881 gccggttcac cggcccgctg gacaccgccc tggccgagct ggacctccac cggcgggtca
     2941 ccgtcgtcct tcccagccac ctggccgcga tggccctcgc cgcgcgcagc gacgtcgtct
     3001 gcctcatccc cgccgcgctc cccggcgccg ccccctcacc cctcaccgat gccgccacca
     3061 ccttcggcct gcacctcctg gacattcccc tgccgctgcc gccgatgacc ctcggcatgg
     3121 cctggcaccc acgccacgct gctgacggag cccaccactg gctgcgcgac gccgtccgcc
     3181 gcaccttgcg ggctgccggg agcccggcca cctgacgcgg cgcgcgccga gggcagcgtg
     3241 cgccgggagc agcgcgcgcc gaggactgcg ggctccctcc ctggcccgcc ccctcaggcc
     3301 cgcagccagg tacggcggtt gcgggcgacc gtgtagacgc gttcgacacg gtcgggggcg
     3361 aggaccccgc cgagccgtcc gagcaggggc acctcgcgtt cgtcccggat cacccgtacg
     3421 tcgtcccgca gtgccgcgtc cagctccacg tccaccgcgc cgacgaacgc cagcgccggc
     3481 cgcacctcga cgcggaaccg gcagccgcgg gtcaggacat aggacgcgcg ccgggcgtcc
     3541 tgcctgatct cgcggacgta cggccgtgac tggtgcccgc ggctgatggc cacggcctcc
     3601 tccgcgaccc gcacccgggc cccgcggacg tgatgggtgg cgacgcacag cacgccgccg
     3661 ggcccgatga gcagatggcc gatggccgct gcgggcggca cggggacgga gtggatcacg
     3721 tgccagccgc ccgccgtcag ctggtccagg gccgtgccga cggcctgctc gccgaggagc
     3781 tgccggtgcc aggcgtcctc gggcgggcgc cggccgagca ggcgggcgca ccacaaggcc
     3841 gctctggagc gcggctgctc ggcgaggcgg acccgcaggg cctccccggg acggttcggg
     3901 gcgaggtcgt cgtccggcgg caggaacagc cggtccgggt cactcgcgta ccgcgtctcc
     3961 tgcttgccct cctccgctcc gggctcagga cggatgtcgc tcatacccat caccccccga
     4021 tggccatggg agcaggcgcc cggcccctgg gcaagaggta cgcagcggtg cgcgggcata
     4081 ccgggggcac cgggaagggt tggccggggg acgacgtacg gttacgtcct gtgctctcct
     4141 ctctcctggg tttcaccgcg gtggccgtcc tgctgaccgt ttcgccgggc cccgacttcg
     4201 ccgtcgtgct gcggacgtcc ctcggttcgg ggcggcggtc ggcgctgtgc tccgcgctcg
     4261 gcatagccgc gggctgcgtc gtgtggggac tggccggagc ggccgggctg accgccatgc
     4321 tgtcggcgtc gcagacggcg tacgacgcgc tgcgcgtggc cggtgcgctg tatctgatgt
     4381 ggctgggctt ccaggcgctg cgcagcgccc gccgctcctc gaaggccgcg cgcgacgccg
     4441 cggcgtctgc cactgccgcg gcgggcgccc cgctccctga ccccgccgcc ggccacgccc
     4501 ctgtggccgg ctccggcccc gctgtcgacc agcagtcgcc gcttcaggcg ttccgtaccg
     4561 gtctgctgac gaatgtcctg aaccccaagg tcggggtcgt gtacatgtcc ctgctgcccc
     4621 agttcatccc gcacggcgcg ccggtcgtga ccacgacact gctgctggtc gccgtgcacg
     4681 tcgcgctggg cttcgtctgg ctcggcggga tcgccctggc ggtgcaccgg gcgcgcgcct
     4741 tcttccagcg cccccgcgtc cgccggcgca tggaccaggc caccggcggc gtcctgctcg
     4801 cgctgggcgc cgcggtcggt gtcgagaccg cgcgctgaac cgcccgccgg gccgcccgcg
     4861 cccggcctcc gggcgcaacc gtccggcacg gcacgtcgca agctcaaatc atgctctgaa
     4921 tccgggcccg ccggggttgg cccgggaccg tcgtggcgat gagtaccggc accacggggg
     4981 tgcacgaacg gggggaaaac atggaacatc tcggtacggg catcggctgg cggcccgaga
     5041 tcgccgacga gacagcggaa ctgcccggcg tggactgggt ggaggtcgtc gcggagaaca
     5101 tctgccccgg tcatctccca cccgcgctcc aggccctgcg cgaacggggc acgaccgtgg
     5161 tgccccacgg cgtctcgctc ggactcggcg gcgccgaacg ccccgacccg ggcaggctgg
     5221 ccgcgctcgc ggaacgcgcc gaggcactgc gcgcgccact ggtcaccgag cacatcgcgt
     5281 tcgtacgggc cgggggcccg ctgaccgcct cccccgcgat ggaggcgggg catctgctgc
     5341 ccgtgccgcg cacccgcgac gcgctggacg tgctctgcga gaacgtccgt atcgcgcagg
     5401 acgcgctgcc cgtgccgctc gcggtggaga acatcgcggc gctggtcaac tggcccggcg
     5461 aggagatgac cgaggggcag ttcctcgccc aactcgtcga gcgcaccggc gtacggctgc
     5521 tcatcgacgt cgccaacctc cacaccaatc atgtgaaccg gaacgaggac ccggccacgg
     5581 cgctggacga actgccggtc gaggccatcg cgtacgtgca cgtggcgggc gggatcgagc
     5641 gggacggggt ctggcacgac acccacgccc acccggtgac ccggcccgtc ctggacgtac
     5701 tggccgcact ctgcgcccgc gtcaccccgc ccggcgtcct gctggagcgc gacgaggact
     5761 tccccgcgtc cggcgaactc gccgccgaac tcgacgcgat ccgcaccgtg ctgaaggagg
     5821 ccccgccggt cccggctccg cgccgaacgg gaggcggccc cgccgccacc acaacgacga
     5881 tctccaccgc aacagccgcc accgcaacag ccaccaccgc cacggtcact cccaccaccg
     5941 ccgcccgtca gcggctggcg gtcgggcaga ccgcgctgct ctccgcgctg gtcgccggaa
     6001 cacctgcgcc ggagggcttc gaccggcggc ggctgctcgt gcagagccgg gccctggccg
     6061 ccaagcgggc ctccgtcgtc gccaagatcg cgccggagct gccggacatc ctcggcgagg
     6121 actaccggcc cggcttcctc gcctacgcgc gccaccggcc gctgcacaac ggctaccgcc
     6181 aggacgccat ggacttcgcc gtccacctgc tggccgacgg ccggcccacc gacccggacg
     6241 cccgccgccg cctgacgcag tggtggcggg agcgcgcggg gtccaaaccg ccgggcggcc
     6301 cggtgaaccg cctggcccgc gccgtacgac acgccctgca ccggaggtga ccgagatgcc
     6361 cgctcttttc gtgaccctgg cagtcgccgc ctcttcgctg ctgctcgtcg tccgtaccgt
     6421 ccacgtcgtc ctggcgacgc ccaagctcaa gatcttcggc ccgcccgacc ccgaactctg
     6481 ggaggtcgcc ttcctctccg gcggcccagc gcgggtggcg gacgcggcga tagccggtat
     6541 gcaccaggac ggccggctcg ccgtgggcgg gcccggcgtg gtgtccgtac ggcaggcggt
     6601 ggcgcacgac ccggtcgaga cggccgtact ggactcgttg gcgcggaccc cggacggctc
     6661 gctgggcacc ctgcgccgcc atgtcatgcg ggctcccgcg gtccaggcca tcggcgaccg
     6721 gctcgcgaag cgcggccaga tgcggcaccc gcgcaccggc cgcccctggc gccgactggc
     6781 cggctggcag gccggcatct gtgcggcggg cttcctgctc gtcctcctct tcaccctcat
     6841 cggagccatc gacagcaggg gttcgggcgt ccccttcgtc gtccggatcc tgcccgcggc
     6901 cttcgtcggc atcgtggtcg gcggggtgtg ccgcagggtg ctggggcggc gggtcacccg
     6961 ggccggtgtt tcggcggtca ggaagttccg tacggcgaac gcggctcggc tggtgagcga
     7021 cccgacccag tccgccgctc tgatcgtggc cctcggcagc accgcgctgc tggtggacga
     7081 cgtgctgcgc gaccacctcg tcgaggcgca gggctcggcg gcgggagcct ccgccggtgc
     7141 gtattccggt acttcctcca ccgcgtcgag cagttcgtcg aacgcgtcct gcggttggaa
     7201 cgtgccggac gacggctggc acacctcttg gtgcgggacc tcgtgcggtg gcgggtcggg
     7261 gtcgagctgc ggcagttcgt cctgcggcgg cgggagttcg tcgtgcagcg gcgggagttc
     7321 gtcgtgcggc agcagctccg ggtcgagctg cagttcctcc tcttcgtcct gcggcggtgg
     7381 cggctgcggc ggcagctgac ccgaggaaca ccggcggcga ccgtccccgc gaaggggcgt
     7441 gcagctctgt gcaatgacat gggtccggtg tacgggcgcg ggtgggccgg gcgcgggcgg
     7501 accggccgcg cccggttcca cgtcctcgtg gccgacgccc ggcgctgaac ggaatcagcg
     7561 gccgggtgtc gataaatgaa caggtgccct aaaactgttc gcaacaattc cggcctgccg
     7621 cggcgaatca tctcgaaaga gtgaatcagt tccgcttttg cggcctgttg gcggtccgta
     7681 cggagccgct agcatcgatt tcgcacgccc cgaaccggcg cctgccccga cgttgttcat
     7741 tcccctacgg cggcgctgtg agatgcgaac gaccacacat cgaacggatt tggagaatag
     7801 cgtatgcccg ttgaggaatt cccgatccaa cagaccggcg ggtccctggt gccccattcc
     7861 gtcgcggtcg acccgcaggg ctggctgtcg gtgaccaccg acaacagcgc cctggtgcag
     7921 atcagcagga gggatccgac cgcccagacc gtgcacaggg acgccctccc ggagttcgcc
     7981 gccggaattc tcaaggatcc gacgggccgg aaggtcgtct ggctcgcctc gccgacggga
     8041 ccgcaggatt ccgacccgat ccatgagttc gatgtcgaag cgagaagggt catcaacaag
     8101 cacggcctcc aggacggcac ccgggcgcag gccatcaccg gcgtgcgcgt gaagaccggc
     8161 tccggcccgt cggatgcgtc caagggctac gtggtgttcg ccgagccgac gcacacgagt
     8221 gtgggctacg tcgaggtcgg caaagacggc gcgaaccacc atccgctgcc caccggcacc
     8281 gacgaattcg acacctggct ctggtcggtg gcggccaccc tcgatacgac ccggacgaag
     8341 tgcacctact gggccaccgg ccagaagcac gacgagccgg agcgaaagac caacggcctg
     8401 tacacgtaca cgccggccga cgacgacacg tggcagcgga ttccgctccc gaacggcgcc
     8461 gagcaagtgc ccatccacgt catcgccggt gtcctccggg aggacgagcg gcgcgatccg
     8521 tacctgtgga tcagcgcgcg caacccccac cagatcctgc gctacgacat caggaacaag
     8581 tcatgggcgt cgagccccac cctcaccggt gaaccgcggc agctggcctt cggccccgac
     8641 ggcaacatct gggtggccgg caccgaccag atccactgct tcgagaagga cgccgcgctg
     8701 cgctcccttc ccgtccccgc actgccctcg ggcagcgagg cccacggaat ctgcgtcgac
     8761 ccgtcggacc aggtcctctg gtacaccaac cgcaagacca acacgatcgg caaataccca
     8821 ctcccggacg ggtccagggc cacgaccggc aagacccagg tgctggcaca gccgcagaag
     8881 gaaacctgcc cgggtgccgt ggcggaagtg cccatggtgg ccgaattcgt ggcgctcggc
     8941 gaaccgaccc cggaaatccc cctcacctgc cggatcatct ccgaagaagc gacgttcacc
     9001 gacgggagcc gtgagcgcgt catcgccacg gacccggtcg gccaggtcgc cttccccgcc
     9061 gttctggcag gcaaggtgga agaggatgtc ttcctgaaga tgagctgggg cgacaacgaa
     9121 ccgtccacca ccgtcatcct ccgcgtgcgc aacgacgagg agtaataccc ccgcacccct
     9181 tcaaaaccct ccttcagcca gccgggccgt ctcggtgcgc ggcgtgtggg ttggtgtggt
     9241 tgacgagcag gtgccgcacc cggcgcggca ccgctccgga tctccagccg gtcgccataa
     9301 cttcggaagg gccgtgccac ggcctccgat acgcctgaca cccctgggca accggtcaac
     9361 gtatcagcgg gccatgggtg ttgacggggc ttccccgaac gggcgacagg atcagcgcag
     9421 ggcgcactag cgtgcggtgc cgcaccagga ggcaccatgc gatccgtaac cggcaccggc
     9481 ctcgtcgtcg ccgcgctcgc cgcgacactt gccgcgctgc tcttccctat ctggtcgttc
     9541 gccgaccggc ccggcgcggg cggggacgtc aacacccaga ccgtcgccac ctccttcggc
     9601 ccgctgtccg cgctcgaccg ggacttcgtg aaccgcgtac ggctggcggg cctgtgggag
     9661 ctgcccgcgg gccagcaggc ccagcagcgc gcgaccgtcc cggcggtacg ggaggcgggc
     9721 gaccatctcg tcgacggcca cgcctcgctc gaccggcacg tacgcgaggt ggccgcgcag
     9781 ctcggcctcg cgctgcccga ccggcccaac gcccagcagc agcagtggct gtcggagctg
     9841 agcgccgccc gcggggccgc gtacgaccgc aagttcgcga acatcctgcg gctggcgcac
     9901 ggcaaggtct tcaccgtcgt cgcgcaggtc cgcgcgacca cccgcaactc gctcgtccgc
     9961 ggtctggccg acgacgccaa ccgcacggtc ctggaccaca tctccatgct ggagcgcacc
    10021 ggtttcgtcg acttcgacgc catcgcccag gacgcggcca ccgcctcacc ggcgccgagc
    10081 ccctcggccc cgccgccgtc cctcgacgcg gcgcccggca caccgccggt gcccgtcact
    10141 ccgtcaccga cgtattcgct gcctccgccc gcgacgccac ccgccaccgc caccggcggc
    10201 gccaccaagg ccccggaatc caccgagccc cccggcggcc ggcgtcaaca cccctgagag
    10261 aacgggaaga agtgatcgcc catgcccgac catccgcact ccggcagtcc caaaccgccg
    10321 cagcctcccc gcccttcgcc gccaccgcgg ccgtcgccac cgcccccgca gcgcccgcgg
    10381 caccggcgcc gcctcggcac caagcacctc gtgctgatcg ctctgctcgc cgtgctcggc
    10441 tcggccggca ccttcgcctc cgtgaccgga aacgcctcgg ctcacaacgc cactcccccg
    10501 tccggctccc cgcccgtcga tccgtcatct tcaccgtcgt cacccagcgt gacacagccc
    10561 actgacaacg cctcggcaac caccccggca accgccccca ccacgggttc cgccgagcgt
    10621 cctggcaccc ccgccgacac ccccgccgac acccccgacg gccccgcggg ccccaccgga
    10681 tcccgcggcg ccggggattc ctccggcacc ggccaaagcg tcccggcagc cgccgacttc
    10741 accgacatcc gctccgtacg ccccggcccg cagcccccgc ggggcggccg cgacgcgtcc
    10801 accggcacct tcaccagcag ctgcggccgc aacgaggacc ggcacaacaa ccccgacaac
    10861 ttcatcgtca ctccgggtgt cgccaacggc gcccaccacc tccacgacta cgtcggcaac
    10921 cgctccacgg acgccttctc caccgacgac tcgctgcgcg cggccggcac cacctgcgcc
    10981 cgcggcgaca agtcggcgta cttctggccc gtactgcgcc gcctcagcgg ccgggacggc
    11041 ccgagcagcg gcaacggcca ggaccccgcg gacgggaacg tcggacaggt gctgacgccc
    11101 cgctcggtct cgctgacctt ccgcggcagc cctgcgggca aggtcgccgc catgcccgag
    11161 ggcctgcgcg ccatcaccgg cgacgcgaag gcgttcacca acggcaccgc caatgcgcgt
    11221 gcgcagtgga cctgcaccgg attcgaggac cggcggctgg ccgacaagta cccactgtgc
    11281 cccgggggca gccaggccgt acggctcctg gacttcccca gctgctggga cgggacgaac
    11341 aaggacagcg
//
