LOCUS       NZ_LWKZ01000024        11275 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold4, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000024
VERSION     NZ_LWKZ01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1153214)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153214)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 448220
            Orig. end                         :: 459495
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11275
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="4"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..11275
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11275
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6275](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6275
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            514..1473
                     /locus_tag="A6A06_RS25265"
                     /old_locus_tag="A6A06_18375"
     CDS             514..1473
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356079.1"
                     /locus_tag="A6A06_RS25265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_18375"
                     /product="MoxR family ATPase"
                     /protein_id="WP_073763610.1"
                     /transl_table=11
                     /translation="MTENDAWRLFRGDRTPRRAKLPPAPPWRQFTAPGAADGGGRQERP
                     YLIGRAQADVVSAALHLRRPLLVTGHPGTGKSSLAHAIAYELSLGPVLQWAVNSRSVLK
                     EALYDYDAIGRLRETHLRQEHTGPEPDIGTYIRLGPLGTALVPRDVPRVLLVDELDKGD
                     VDLPNDLLMVFEEGEFEIPELSRLPEDRSEVEVLLHGTDSGRVRVTRGLVRCREFPVVV
                     ITSNGERDFPPAFLRRCVRLALPEPDEDQLRRIVAAHMGEPALRGMDDLLHTFVNERAH
                     GELATDQLLNAVFLRKGGVDLDAQGLLRAVLHRLGGTV"
     gene            1470..4883
                     /locus_tag="A6A06_RS25270"
                     /old_locus_tag="A6A06_18380"
     CDS             1470..4883
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041121.1"
                     /locus_tag="A6A06_RS25270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_18380"
                     /product="SAV_2336 N-terminal domain-related protein"
                     /protein_id="WP_079194325.1"
                     /transl_table=11
                     /translation="MTVPGGGAGFEQVGAVTGYDLSTLELLDVLWLAGRLPRDGSVPLA
                     ALPAQPAGPHSGEGRVPPPGESGRLPDGTAAGASGRARAPAHAGPARRRASPSQLAGAL
                     HAAASAGGAVEFSGTAARAGHGDAGALPVRIPENKALGHEELRLSRALRPLKQRRPRRD
                     RQELDDAATVAAMAETGLPDVVTRPARERWLDLTLLIDDGVSMLLWRRLAAELRALFEQ
                     LGAFRDVRVHGLDSRSPDGPRLRARPFDPDPALLSPASVADPSGRSLILVVSDGVGSCW
                     RDGTLHTALERWARRGPTAVVHALPAHMWSGSGLRAEPWRVTTRQRGAANDTWDVANAL
                     LPAGFADDFPGVPVPVLEPRRAAVAAWARLVASPGASAELPLLSPPTVPGVRPEAGTRT
                     PDAADAVLRFRDAASAPAYRLAAHLAAVSPITVPVMRLVQAAVPWPAETSHLAEVFLGG
                     LMRRAGPADAAQPQHLSFEFAEGTQEILLDTASPIELLRTTRAVTERLARLVGRSPDFP
                     AWLAHPSGTGELLPGTRPFAWLEDRLLTHLGARPMAQIQALPPETYEEARLPDTSGTAP
                     SWLPLRLQDLHSLGPYTLRLRGESSGVPNAYIGENETGAQVLLRVSQSVKWPMARELLG
                     VEWRALHRMGGVYAPEVVASDMEGPVPWIALRLDRLSTELPAPTLRALLRAAGALYDSP
                     LFPWLGWHLARAVSRCHRNNIVHGALTPGTVLVTEETVRILEWTSARIDGESSASPLAM
                     PTRSPHRAPEVTSWGESRIAAGDVYALGTILVDAATGRSWRWYRRDVLLTQEEFLQLDG
                     GLRDILLRCIDDEPEARPTAAEVAEVFAGYLPESVTRAGADDRPSGPPPSLSPELPEPD
                     RSDTDAERDQLLATVRAPVSGPLHIALLALKDRVGCTTTTAVLGALLAEQRREPVLAVD
                     ADRHAGSHIRIARRVYRDTDARLTDLARSAGHVRGFGDFRLFVSQHRSGLLVLANDSVH
                     HGSGTPAQFTDRDYATVTALAEPYCPVTLADTDRFPQAVLDRTHRVVIVTRADPAGLTH
                     AERSMDRLLTLGLPHLAADAVVVLNHSRPEAGWTLNPATVRGLSSRCRGVVMVPRDGHL
                     ATGSVVELSWLAPETYRAYLRLAALLLDRW"
     gene            5001..6275
                     /locus_tag="A6A06_RS25275"
                     /old_locus_tag="A6A06_18385"
     CDS             5001..6275
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /locus_tag="A6A06_RS25275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_18385"
                     /product="tyrosinase family protein"
                     /protein_id="WP_073763611.1"
                     /sec_met_domain="melC (E-value: 2.2e-21, bitscore: 72.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRRDVFAGATAAGAAALLPTAAGPRGSRPYTERYLGRTIRIDAP
                     HGTARVTIDGRALDLMRMGADAYLSPLCHYGFDRTPLAAARRAVRRLRGARLLPPPPLR
                     SWPRPVVPPGPAPPARPYVRKNHLALTAQERHRFVDAVLEAKRRGVYDALVAVHIRLNS
                     HDYIHRDHGRRTAHLTPSLLPWHRQFLLYFEQELRAIDPAVTLPYWDWTSDRGRESPLW
                     ADDFMGGDGRPGDRQVTAGPFARRNGWRLGVDVRPTGHDVAWLNGHYTQDDRHFLTRAL
                     RRPGVLSTARQLEAALALPVYDCPPWNHLSGQSRSQRSFRNHLEGYAKFPWERDWGKLH
                     GSAHAFVGGHMDFIGSPNDPVFFLHHCFIDKIWAEWQRRHPGVPHYLPLEPAPDVPGLH
                     TPLAPWHTMSPADLVDHTRFYRYDA"
     gene            6340..6834
                     /locus_tag="A6A06_RS25280"
                     /old_locus_tag="A6A06_18390"
     CDS             6340..6834
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981960.1"
                     /locus_tag="A6A06_RS25280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_18390"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_073763612.1"
                     /transl_table=11
                     /translation="MTTDYATYIAGLPRVLAGAGVIFRDADGRVLYVEPNYRDTWNLPG
                     GTIESDEGESPRQAARRETAEEIGLDLEPGALLAVDWVRGTARPPLVSYLYDGGVLDAE
                     QLAAVRLQESELLSWSLMTWEEAEPRLSPSMALRMRAALDALARGRGPVELEDGVAPAA
                     P"
     gene            6868..7803
                     /locus_tag="A6A06_RS25285"
                     /old_locus_tag="A6A06_18395"
     CDS             6868..7803
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /locus_tag="A6A06_RS25285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_18395"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_073763613.1"
                     /transl_table=11
                     /translation="MTLLPDSAVRPGRLAVGVVLPSLTVQREQKLPLTEAARHAELAGL
                     DLVAHRGGPVSGTPSLDAAVAVAAAAAATERITVVGEACAPALLPPVRAAEQVASLQYV
                     ADGRLVLAVGTDAGHERWAAAGVPYDERERRTDAVLRLLPRLLAGERVALPYEPGYAAA
                     ESAPAVPMPPVWIGGTSRTAVRCAARLGDGWFPSLASAAEVAEGTVQLAELAAASGRPA
                     PAVVVGTTGALGGEPGLPGRADIAADLAAAHGGRPGRFADVPVTGGCREAAEHLHRYCG
                     AGVTRVLVDIAGGDWRNQVDLLADVRDLLR"
     gene            complement(7861..9936)
                     /locus_tag="A6A06_RS25290"
                     /old_locus_tag="A6A06_18400"
     CDS             complement(7861..9936)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742451.1"
                     /locus_tag="A6A06_RS25290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_18400"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_073763614.1"
                     /sec_met_domain="PIG-L (E-value: 3.7e-17, bitscore: 59.1,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEHIGTGGPLRPTRRAVALGLAALTVAGCGDRAERPRPGTAPAG
                     KRGKVSAAADGSSGARVLQVVAHPDDDLYFLNPDLQQSIDANDQLVTVYLISGEADGRN
                     KAPGSTTAPKADRAAYAGARRQGLRQAYSLMATGRTDARWELKADKLPDGTLIETDTLA
                     GHPGLQLVFLGLLQHSSHGGGRSRGLVDLWADPAMVTSTHVTTGSPVRDPHRVTRASLT
                     DALVHLLEQYRPTLVRTMDPDPDMQVHDKKHRLHHDAHGYSDHPDHTAAALFTHAALAR
                     YRSRGAGRSYLVTAYRGYYNERWPDNLPRQVVRGKADVLNAYGGNPGSCGFAAGCGDYD
                     VGRDRSFGTGWLQRTSPRHPAAGPRTRPAPDGRLTAFAVLGGQAAMWQETGRGSGTWTA
                     PRLLGGDGLLPGLTAELTEDGRWQLFAERIAALGRTRPDNRREIVTAGQTRKGGPFGAW
                     SSLGNPETDPDHGRRVGGPVTVRTADGTTCLFVRTWAKGVAVRRHTDAGWDGWTDLGGA
                     EVQEGLSAVADREGRVHLFGSAHDTVHHWAQQRPGGDFTLVPTGLPAPADPPTALARPD
                     GTILLAFREAGSARPLVHTLPAGGGTWRPERVGLDARGYGMLTLHPARGGGVLLAARNN
                     NGSTSLATIGTRKAPRWSTVAGRAVGNVCLTGAAGARPVLVRLGPDATLHSETVAG"
ORIGIN
        1 agaccgcccg gcacgacctg agccggcgct gggaggcccg gcggcgcacc gggccgctga
       61 tcgccgacga gcaggtcacc agcgaccggg agctgatggg gatgctgaag accacccacc
      121 gggaccaggg cagcgtcgtg gtgcacggac cgcccaagca ccgggcactg ttgctgaacg
      181 tgtgcctgtt catgggggtg ccgatcgtgc tgtgggaccg ggaggcggtc gaccaccggc
      241 aggcggcgcg cctggaaccg tcgcgcccgt ccggtccgtt ggaagaactg ccggagcggg
      301 tacggcattt ccgggtacgg gcccacatgg acccgccgca ggcggcccgg cccgcgctcg
      361 tctgggagga cgccgagctg ccgctgccgg acgagctgct gctggccgat ccgggcgcgg
      421 ggcccgaggg gtgaggggag gggcgtacgg accgcggtac gggggcgcgg gccgcggcgg
      481 aaaccgacgg aggacggcgg gagcggatcg gcgatgacgg agaacgacgc gtggcggctc
      541 ttccggggcg accgtacccc ccggcgggcc aagctgcccc cggcgccgcc ctggcggcag
      601 ttcacggcgc ccggcgccgc ggacggcggc gggcggcagg agcgcccgta cctgatcggc
      661 cgggcccagg cggacgtggt gagcgcggcg ctgcatctgc ggcgcccgct gctggtgacc
      721 ggccacccgg gcaccggcaa gtcgtcgctg gcgcacgcca tcgcgtacga gctgagcctg
      781 gggccggtgc tccagtgggc ggtcaacagc cgttcggtgc tcaaggaggc gctgtacgac
      841 tacgacgcca tcggccggct gcgggagacg catctgcgcc aggagcacac cggccccgag
      901 ccggacatcg gcacgtacat ccggctgggg ccgctgggca ccgcgctggt gccgcgtgac
      961 gtcccccgag tgctgctcgt cgacgagctg gacaaggggg acgtggacct gcccaacgac
     1021 ctgctgatgg tcttcgagga gggcgagttc gagattccgg agctgtcccg gctgccggag
     1081 gaccgcagtg aggtcgaggt gctgctgcac ggcacggaca gcgggcgggt gcgggtgacg
     1141 cgcggtctgg tgcgctgccg ggagttcccg gtggtggtga tcacgagcaa cggtgagcgg
     1201 gacttccccc cggcgttcct gcgccgctgt gtacggctcg cgctgcccga acccgacgag
     1261 gaccagctgc gcaggatcgt cgccgcgcac atgggcgagc cggccctgcg gggaatggac
     1321 gacctcctgc acaccttcgt caacgagcgg gcgcacggcg aattggcgac ggaccagctg
     1381 ctcaacgcgg tcttcctgcg caagggcggc gtggacctgg acgcccaggg gctgctgcgg
     1441 gcggtcctgc accggctcgg cggaacggtg tgaccgtgcc gggcggcggc gccggtttcg
     1501 agcaggtcgg tgcggtcacc ggctacgacc tctccaccct ggagctgctg gacgtcctgt
     1561 ggctggccgg gcggctgccc cgcgacgggt ccgtgccgct ggccgcgctg ccggcgcagc
     1621 cggccggtcc gcactccggc gagggccggg tgccgccgcc cggggagtct ggccggctcc
     1681 cggacggtac ggccgcgggc gcgtccggcc gggcgcgagc cccggcccat gccggaccgg
     1741 cccggcgccg ggcgtcgcct tcccagctcg ccggtgccct gcatgccgcg gcctcggcgg
     1801 gcggcgccgt cgagttctcc ggcaccgccg cgcgggccgg gcacggggac gccggggcgc
     1861 tgcccgtacg cataccggag aacaaggcgc tggggcacga ggaactccgg ctcagccgtg
     1921 ccctgcgccc gctcaagcag cggcggccgc gccgcgaccg ccaggagctg gacgacgcgg
     1981 cgaccgtggc ggcgatggcg gagaccgggc tgccggacgt ggtgacgcgg cccgcccgcg
     2041 agcggtggct ggatctgacg ctgctgatcg acgacggcgt gtccatgctg ctctggcgcc
     2101 gcctggccgc cgaactgcgg gctctgttcg agcagttggg cgccttccgg gacgtacggg
     2161 tacacggcct ggacagccgg tcgccggacg gcccccggct gcgggcccgc cccttcgacc
     2221 ccgatccggc gctgctgtcg cccgcgtcgg tggccgatcc gtccggccgc agcctcatcc
     2281 tggtcgtcag cgacggcgtc ggctcctgct ggcgcgacgg tacgctgcac accgccctgg
     2341 agcgctgggc gcgccgcggc cccaccgccg tcgtccacgc gctgcccgcc cacatgtgga
     2401 gcggttcggg gctgcgggcc gagccgtggc gggtgacgac ccggcagcgg ggcgcggcca
     2461 acgacacctg ggacgtggcc aacgcgctgc tgcccgcggg cttcgccgac gacttccccg
     2521 gcgtcccggt ccccgtcctg gagccgcgcc gcgcggccgt cgccgcctgg gcccggctgg
     2581 tggcctcgcc cggggccagc gccgagctgc cgctgctgtc cccgcccacc gttcccggag
     2641 tgcgcccgga ggcgggcacc cgcaccccgg acgcggcgga tgccgtactg cgcttccgcg
     2701 acgccgcctc cgccccggcc taccggctgg ccgcgcacct ggccgcggtg tcgccgatca
     2761 cggtgccggt gatgcggctc gtgcaggccg cggtgccgtg gcccgccgag acgtcgcatc
     2821 tggcggaggt gttcctcggc gggctgatgc gccgcgccgg gcccgccgac gccgcgcagc
     2881 cgcagcacct cagcttcgag ttcgccgaag gcacccagga gattctgctg gacaccgcct
     2941 ccccgatcga gctgctgcgc accacgcgtg cggtcaccga gcggctggcg cgcctggtgg
     3001 gccgttcgcc ggacttcccg gcgtggctgg cgcacccgtc cggcaccggt gaactcctgc
     3061 ccggcacccg gccgttcgcg tggctggagg accggctgct gacgcacctc ggtgcccggc
     3121 cgatggcgca gatccaggcg ctgccgccgg agacgtacga ggaggcccgg ctgccggaca
     3181 cgtcgggtac ggcgccgtcc tggctgccgc tgcggcttca ggacctgcac tccctcggcc
     3241 cgtacaccct gcggctgcgc ggcgagtcca gtggtgtgcc caatgcgtac atcggcgaga
     3301 acgagacggg cgcgcaagtg ctgctgcgtg tctcgcagtc cgtcaaatgg ccgatggcca
     3361 gggaacttct cggcgtcgag tggcgtgcgc tgcaccgcat gggcggggtc tacgcccctg
     3421 aggtggtcgc cagtgacatg gagggtccgg tgccgtggat cgcgctgcgg ctggaccggc
     3481 tcagtacgga gctgcccgcg ccgacgctgc gcgcgctgct gcgcgcggcg ggcgccctgt
     3541 acgactcgcc gctgttcccg tggctcggat ggcacttggc gcgcgcggtg agccgctgcc
     3601 accgcaacaa catcgtgcac ggcgcgctga cacccgggac cgtgctggtc accgaggaga
     3661 cggtacggat tctggagtgg acctcggccc gtatcgacgg cgagtccagc gcgtcgccgc
     3721 tggccatgcc cacccgttcg ccgcaccggg cgccggaggt cacctcctgg ggcgagtcgc
     3781 ggatcgccgc gggcgacgtg tacgcgctcg gtacgatcct cgtcgacgcc gcgacgggcc
     3841 ggtcctggcg gtggtaccgg cgcgacgtcc tgctcaccca ggaggagttc ctccagctgg
     3901 acggcggcct gcgggacatc ctgctgcggt gcatcgacga cgagcccgag gcgcggccga
     3961 cggccgccga ggtggccgag gtgttcgccg gctacctgcc ggagtcggtc acccgcgccg
     4021 gtgcggacga ccgcccctcc ggcccgcccc ccagcctgag cccggagctc cccgagccgg
     4081 accgcagcga caccgacgcc gaacgcgacc agctgctcgc cacggtccgg gccccggtat
     4141 cgggccccct gcacatcgcg ctgctcgccc tcaaggaccg ggtgggctgc accacgacga
     4201 cggccgtgct cggcgcgctg ctggccgagc agcgccggga gccggtcctg gccgtcgacg
     4261 cggaccggca cgccggcagc cacatccgca tcgcgcggcg cgtctaccgc gacaccgacg
     4321 cccggctgac ggacctggcg cgttccgccg gacatgtgcg cggcttcggc gacttccggc
     4381 tgttcgtgtc ccagcaccgg tcggggctgc tggtgctggc caacgactcc gtgcaccacg
     4441 ggtcgggcac gcccgcccag ttcaccgacc gcgactacgc gacggtgacc gcgctggccg
     4501 agccgtactg ccccgtcacc cttgccgaca ccgaccggtt cccgcaggcc gtgctggacc
     4561 gcacccatcg cgtcgtgatc gtgacccggg ccgacccggc cgggctgacc cacgcggaac
     4621 ggtccatgga ccggctgctc acgctcggtc tgccgcacct ggcggcggac gcggtggtgg
     4681 tgctcaacca cagccgcccg gaggccggct ggacgctgaa cccggcgacc gtgcgcgggc
     4741 tcagttcgcg gtgccgcggt gtggtcatgg tgccacgcga cggccatctg gccaccggca
     4801 gcgtggtgga gctgtcctgg ctcgccccgg agacctaccg ggcgtatctg cggctggccg
     4861 cgctgctcct ggaccgctgg tgagacgggg cctccgcgtc acctgttcgg gtgcattcgg
     4921 ccggggaagg cgtccgggcc cccggccggg gtgaagctcc ggacaagggc gaaggtccgg
     4981 acagaggcga agggggcccg gtgcgacggc gcgacgtttt cgcgggggcc acggcggccg
     5041 gtgccgcggc cctgctgccc acggcagccg gcccgcgcgg ctcgcgcccg tacaccgagc
     5101 ggtatctggg ccggacgatc cgcatcgacg ccccgcacgg cacggcccgg gtgacgatcg
     5161 acggccgcgc actggacctg atgcggatgg gagcggacgc ctacctgtcc ccgctgtgcc
     5221 actacggatt cgaccggacc ccgctggccg ccgcgcgccg cgccgtgcgg cggctgcggg
     5281 gcgctcggct gctgccgcct ccgccgctcc ggtcgtggcc gcggcccgtg gtgccgcccg
     5341 gccccgcgcc ccctgcccgc ccgtacgtcc gcaagaacca cctggcgctg accgcgcagg
     5401 agcggcaccg tttcgtggac gccgtcctgg aggccaagcg gcgcggcgtg tacgacgctc
     5461 tggtcgccgt ccacatccgg ctgaactcgc acgactacat ccaccgcgac cacgggcggc
     5521 gcaccgcgca cctgacgcct tccctgctgc cctggcaccg gcagttcctg ctgtacttcg
     5581 aacaggaact gcgcgcgatc gaccccgctg tcaccctgcc gtactgggac tggacgagcg
     5641 accgcggccg ggaatcgccg ctgtgggccg acgacttcat gggcggcgac gggcggcccg
     5701 gcgaccggca ggtgaccgcg ggccccttcg cccggcgcaa cggctggcgg ctgggggtcg
     5761 acgtccggcc caccggccac gacgtggcgt ggctcaacgg ccactacacc caggacgacc
     5821 ggcacttcct cacccgcgcc ctgcgccgtc ccggcgtcct ctcgacagcc cggcagctgg
     5881 aagcggccct cgccctgccg gtctacgact gtccgccgtg gaaccacctg agcggccagt
     5941 cccgttccca gcgcagcttc cgcaaccacc tggagggcta tgcgaagttc ccgtgggaac
     6001 gggactgggg caagctgcac ggctccgccc acgccttcgt cggcggccac atggacttca
     6061 tcggctcgcc caacgacccg gtgttcttcc tccaccactg cttcatcgac aagatctggg
     6121 ccgagtggca gcggcggcat cccggtgtcc cccactacct cccgctggaa cccgcacctg
     6181 acgtgcccgg cctccacacc ccgctggccc cctggcacac gatgtccccg gccgacctcg
     6241 tcgaccacac ccggttctac cgctacgacg cctgagcggc agcaccggac cggtgagacg
     6301 gcccgcaggc cccgccgccg accccactac gctcgacgta tgactacgga ctacgccact
     6361 tatatcgccg gtcttcctcg tgtactcgcg ggggccggtg tcatcttccg cgacgccgac
     6421 ggccgggtgc tgtacgtcga gccgaactac cgcgacacct ggaacctgcc cggcggcacc
     6481 atcgagtccg acgagggcga gtcgccccgc caggccgcgc ggcgggagac cgccgaggag
     6541 atcggcctgg atctggagcc gggcgcgctg ctggccgtcg actgggtgcg cggcaccgca
     6601 cggccgccgc tggtgtcgta cctgtacgac ggaggcgtgc tggacgcgga gcaactggcg
     6661 gccgtccgcc tccaggagag cgagctgctg tcctggtccc tgatgacgtg ggaggaggcc
     6721 gaaccccggc tgtccccgtc gatggcgctg cggatgcggg ccgcactgga cgcgctggcg
     6781 cgcggccgcg gtccggtgga gctggaggac ggtgtcgcgc cggccgcccc ttgacctcaa
     6841 gcccccttga agttgcatcg tcaccccatg actcttctcc cggattccgc ggtccggccc
     6901 ggccgcctgg cagtgggtgt ggtgctgccg tccctgaccg tgcagcggga gcagaagctc
     6961 cccctcaccg aggcggcccg gcacgcggaa ctggccgggc tcgatctcgt cgcgcaccgg
     7021 ggcgggccgg tttccggcac accgtcgctc gacgccgcgg tggcggtggc ggcggcggcc
     7081 gccgcgaccg agcggatcac cgtcgtcggc gaggcgtgcg caccggcgct cctgccgccg
     7141 gtgcgggccg ccgagcaggt ggcgagcctt cagtacgtcg cggacgggcg gctggtgctg
     7201 gccgtgggga cggacgccgg gcacgagcgg tgggcggctg ccggtgttcc gtacgacgag
     7261 cgggagcgcc ggaccgacgc ggtgctgcgg ctgctgcccc gtctgctcgc gggcgagcgg
     7321 gtggcactgc cgtacgaacc ggggtacgcg gcggcggagt cggcgcccgc cgtgccgatg
     7381 ccgccggtct ggatcggcgg cacctcgcgg accgcggtgc ggtgcgccgc gcgcctgggt
     7441 gacggctggt tcccttccct ggcgtcggcc gccgaagtgg ccgagggcac cgtgcagctg
     7501 gcggaactcg ccgccgcctc gggccgcccg gccccggccg tcgtcgtcgg caccaccggc
     7561 gcgctcggcg gggaaccggg cctgcccggc cgcgcggaca tcgcggcgga tctcgccgcg
     7621 gcgcacggcg gccggccggg gcgtttcgcc gacgtcccgg tgaccggcgg ttgccgggag
     7681 gccgccgagc acctgcaccg ctactgcggc gcgggggtca cccgcgtcct ggtggacatc
     7741 gcgggcggcg actggcgcaa ccaggtggac ctgctggcgg acgtacggga tttgttgcgc
     7801 tgacgggcgg tcggcggacc gcagctcgtc acggtgggcc gcagcccgcc acgctgggcc
     7861 tcagcccgcc accgtctcgc tgtgcagcgt cgcgtccggt cccagccgga ccaggaccgg
     7921 gcgggcaccg gcggcaccgg tcaggcagac gttgccgacg gcccggcctg cgacggtgga
     7981 ccagcgcgga gccttacggg tgccgatggt cgccaggctg gtgctgccgt tgttgttacg
     8041 ggctgccagc aggacgccgc cgccgcgggc gggatggagg gtgagcatgc cgtacccccg
     8101 ggcatccagg cccacgcgct cgggccgcca ggtgccgccc ccggcgggca gggtgtggac
     8161 gagcggacga gcgctgcccg cttcccggaa ggcgagcagg atcgtgccgt cggggcgggc
     8221 cagcgccgtc ggcgggtcgg cgggcgcggg cagtccggtg ggcacgagcg tgaagtcgcc
     8281 gccgggccgc tgctgcgccc agtggtgaac ggtgtcgtgc gccgaaccga acaggtgcac
     8341 ccgcccctcg cggtcggcga cggcgctcag cccttcctgc acctcggcgc cgcccaggtc
     8401 cgtccagccg tcccatcccg cgtcggtgtg ccggcggacc gcgacgccct tggcccaggt
     8461 acggacgaag aggcaggtgg ttccgtcggc ggtccggacc gtgacggggc cgccgacgcg
     8521 ccggccgtgg tccgggtcgg tctcggggtt gccgagcgag gaccaggcgc cgaacggccc
     8581 gcccttgcgg gtctgtccgg ccgtgacgat ctcgcggcgg ttgtccggtc gcgtgcggcc
     8641 gagtgcggcg atccgctcgg cgaagagctg ccagcggccg tcctcggtca gttccgcggt
     8701 cagaccgggc agcagtccgt cgccgccgag cagccggggc gcggtccatg tgccgctgcc
     8761 gcggccggtc tcctgccaca tggcggcctg gccgcccagg acggcgaacg cggtcagccg
     8821 cccgtcgggc gccgggcgcg tgcgcggacc ggcggcgggg tggcgcggcg aggtgcgctg
     8881 cagccagccg gtgccgaagg aacggtcccg gccgacgtcg tagtccccgc agccggcggc
     8941 gaatccgcag gagccggggt tgcccccgta cgcgttgagc acgtccgcct tgccccggac
     9001 gacctgtcgg ggcaggttgt ccggccagcg ttcgttgtag tagccgcgat aggcggtcac
     9061 caggtacgag cggccggcgc cgcggctccg gtagcgggcc agggcggcgt gggtgaacag
     9121 ggcggcggcg gtgtggtccg ggtggtccga gtagccgtgc gcatcgtggt ggagccggtg
     9181 ttttttgtcg tggacctgca tgtcggggtc cgggtccatg gtccgcacga gcgtggggcg
     9241 gtactgctcc agcaggtgga ccagtgcgtc ggtcaggctc gcccgggtga cccggtgcgg
     9301 gtcgcgcacg ggtgagccgg tggtcacgtg cgtgctggtg accatggccg ggtcggccca
     9361 caggtccacc aggccgcggc tgcggccacc gccgtgcgaa gagtgctgga gcaggccgag
     9421 gaagacgagc tggagaccgg ggtggcccgc gagcgtgtcg gtctcgatga gcgtgccgtc
     9481 cggcagcttg tcggccttca gctcccagcg cgcgtcggtc cggccggtcg ccatgagcga
     9541 gtaggcctgg cggaggccct gccgccgggc ccccgcgtag gcggcgcggt cggccttcgg
     9601 cgcggtcgtg ctgcccgggg ccttgttgcg gccgtcggcc tctccggaga tcaggtagac
     9661 ggtgacgagc tggtcgttgg cgtcgatgga ctgctggagg tccgggttca ggaagtacag
     9721 gtcgtcgtcc gggtgggcga cgacctgtag gacgcgcgcg ccactgctgc cgtcggcggc
     9781 ggcggacacc ttgccgcgct tgccggccgg cgcggtgccg ggccgcgggc gctcggcacg
     9841 gtcgccgcac ccggccacgg tcagggcggc gaggcccagg gcgacggcgc ggcgggtggg
     9901 gcggagcggg ccgccggtcc cgatgtgctc aggcatgcat ttcgtatcgg ctgggtaccg
     9961 ggaaatacgc ttcgaggaag gggtgcagca ggccggtgcg ctgttcgaag tcgtcctcgg
    10021 tcgacagggt gtcgttgagg cagaagacgc tcttgtcgcg gtgcagcagg agcctgtcga
    10081 gccggttcgc cgcgttgggc cgcgagacgt cgatgtagac gtacggcagg tcgctgggca
    10141 gcgcgcgccg ggtgtggaac gcgtagtagt ggtacagcga ggacgggatg gagatgtcct
    10201 cgtcactgcg gaaccgctgg gccgcggtcc tccggtgctg ctcggcgaac tccccctcga
    10261 tctcggtgag gacgtgccgg ttcagcgcgt gcggtacgtg gcgcatcttg cgcaccggcg
    10321 tggtgccgaa acgttcctgg atcagcgcgc ggttgttctt gcccgcgacg gagaccggta
    10381 cgtcctcggc ggagggcggg ccgaacggca ccagcgcggg cgaggggaag cacttggtca
    10441 ggccgttgcc gaggaagaag tccctgggcc gcatctcggc gcccaggaac acgtcgtcgt
    10501 tgaagtacag gaagtgctcc gagaggccgg ctatgtggtg cagctggctc tcgatggcgt
    10561 gcgagttgaa cgtcggcagc acgccggggt cgctgaagat ctcgcggtgg ctgacgacct
    10621 cgatcccggg ctgccgggtg tcgagccagg cgggcgtctg gtcgtcggtg acgaggtaga
    10681 tgttgcgtac ccacggggcg tacatcgcga gcgagcgcag ggagtagcgc agctcgtcgt
    10741 ggttggcgta gcgcgccgcg ttggccgctt cctcgtgata cgcgtcgccc gagtagcggg
    10801 cccggcgctg ctgccaggcc gggtcggcgc cgtcgaccca ggtgtagacg acgtcgacgg
    10861 ggaaggtgac gtcgtgcggc aggacggtgg tgaactcctc acgggtgcgc accatgaggg
    10921 gcgatacgtc ccgggtcccg ccgggggcgg tgaacatcgc ggccgcgacc tggacggcgg
    10981 gcgcgtcggc ggccacggac tcggtgacgt cgttgcgccg cggagcggtc agccggccgt
    11041 cctcgggctt ccagaactcg atgtcgcagc cgttttcggg gcccagcacc aggcggcggg
    11101 tgcgctccgt gcggtaccag aacagccgca gcgacgtggc gcgggcatgg cggcgccagc
    11161 ccgcggcggg gtaggcgggg gcgacccgtc cgtcaccgtc gtgctgcggg ccgaagtagg
    11221 cgggcagggt gcggcagagc ttctgcaggg cggccagggt gcggtcccgg tcggc
//
