LOCUS       NZ_LWKZ01000023        11908 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000023
VERSION     NZ_LWKZ01000023.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1243511)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243511)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 776959
            Orig. end                         :: 788867
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11908
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11908
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11908
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6908](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6908)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(235..249)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.52"
                     /tool="antismash"
     gene            complement(359..976)
                     /locus_tag="A6A06_RS21545"
                     /old_locus_tag="A6A06_14755"
     CDS             complement(359..976)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662270.1"
                     /locus_tag="A6A06_RS21545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14755"
                     /product="hypothetical protein"
                     /protein_id="WP_079194199.1"
                     /transl_table=11
                     /translation="MPIPSSDRSTCIDALSGALSGLLVALPALVEIFTGETAATSFFLA
                     LSPAFALPLLAGLHRRQGPVTGSFGETAYVVNLIGLGLFGGAAFASNLVLFYFDDGTVE
                     KTLTGPTVPAFIGSAAVFAVGCALFGAAMIRARVFPRGASWSYATALPLFAFLTALPDS
                     ALTSTLHIVVGATLVRLASALRRPAGKTATGAGVLAGQAEPG"
     gene            1233..2384
                     /locus_tag="A6A06_RS21550"
                     /old_locus_tag="A6A06_14760"
     CDS             1233..2384
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="A6A06_RS21550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14760"
                     /product="sensor histidine kinase"
                     /protein_id="WP_073762895.1"
                     /transl_table=11
                     /translation="MAAYVPVPWVSPLLYAAVLVCGLYYAVADLTPGPGPPIWRTAGFA
                     AGLVALVALEVVERRGVVARACLPLLARGSLIAVVVVLDESGLAQALFVLLPFTAYFVF
                     GRCAALALAALCVAGLLIGYALTEPGWYSEAERVSDLLMLCVGLILALSMAAVAIGEQR
                     ARRVLEKYAAQVAELSATAERNRLARDIHDSLGHHLTAVSVQLEIASEFRALEPDSAQR
                     AVDEARLSVKRALGDVRQSVRALRGEETHKSLTVGLAGLACDDEARPHVTVQVDGSEDG
                     YGPAELTALYRAAQEGLTNARRHARATRVTVAVRLTEHAARLVVSDDGCGFVPNGSVTG
                     FGLLGMRERVHMVGGSVHIDSGPGAGTRLTVTVPRAHSEEGVR"
     gene            2381..3031
                     /locus_tag="A6A06_RS21555"
                     /old_locus_tag="A6A06_14765"
     CDS             2381..3031
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662268.1"
                     /locus_tag="A6A06_RS21555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14765"
                     /product="response regulator transcription factor"
                     /protein_id="WP_073762896.1"
                     /transl_table=11
                     /translation="MSEGATPVRVLVVDDQRLIRDGIASLLSIRPGITVVGTAVDGREA
                     VAKVLELRPDIVLMDVRMPGMDGVEAVAVLRGEAPECRTVMLTTFDDEEYVVQAIRAGA
                     CGYLLKDLPADELAHAVRLAYAGVTQLDASVTRHLAACLPCPGPGAEPAVALSPREIDI
                     LRLVAQGRTNREIAAQLYLSEGTVKNYVSRILGRFALRDRTQAALRARELGLL"
     gene            3132..3476
                     /locus_tag="A6A06_RS21560"
                     /old_locus_tag="A6A06_14770"
     CDS             3132..3476
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057451.1"
                     /locus_tag="A6A06_RS21560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14770"
                     /product="hypothetical protein"
                     /protein_id="WP_073762897.1"
                     /transl_table=11
                     /translation="MGGIDPHIHVDAKVVHGDAAARNLLASTFGLVGNVPSTVSTGCGL
                     RVPYAMASPRPDRVTCLACREHARREHLRLAEQVERLSRMLGSAISPAHGKAVADWHRD
                     LAQKFSDAES"
     gene            3522..4223
                     /locus_tag="A6A06_RS21565"
                     /old_locus_tag="A6A06_14775"
     CDS             3522..4223
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016325977.1"
                     /locus_tag="A6A06_RS21565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14775"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_073762898.1"
                     /transl_table=11
                     /translation="MGERAHGPAHRGAGSGPGAFTPDGNAVELYARLPAGAEPEIIARV
                     VPPGASILELGAGAGRVTHVLLERGYRVVAVDESEEMLSRVKGARTVHGSIEELDLGEQ
                     FDAVLLASHLLNTAEPPSCDAMLRSCRRHVAAGGRVLAERQGEDWFRPRRAPAGGERAG
                     ARMRIVSVDRPMPGVSDVRIEYEIDGTRWTHSFRARDVPDATLERMLTAAGLRLVRYLT
                     EDHSWVEAVPV"
     gene            complement(4237..5004)
                     /locus_tag="A6A06_RS21570"
                     /old_locus_tag="A6A06_14780"
     CDS             complement(4237..5004)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.3"
                     /locus_tag="A6A06_RS21570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14780"
                     /product="DUF5825 family protein"
                     /protein_id="WP_073762899.1"
                     /transl_table=11
                     /translation="MSPLATRPPAPVTLALWRERDPAARDIPGVYVGTLDLIGDQVSTV
                     ADLAAHRVQFARLPRTVDLTDRDGAAAVTALVAVRELTAFGIAVDWTLRLPEPEPGTGT
                     GTGTGMGLGTGTPVEWQPLSHLHPPTAVFRGGSGSSDGSGGSDGSGGSDGSGEDIAVRW
                     RDSFHAAMCGYRRGPGFIEVRDRRGGSFKRLVIRTYQHDQLIQSLLRGLPETGVPPSVT
                     ARYLPPGLIHRAGRFLWWTPYRIRRWPLSTTIP"
     gene            complement(5001..6908)
                     /locus_tag="A6A06_RS21575"
                     /old_locus_tag="A6A06_14785"
     CDS             complement(5001..6908)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374931.1"
                     /locus_tag="A6A06_RS21575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14785"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_073762900.1"
                     /sec_met_domain="TIGR03975 (E-value: 7.5e-179, bitscore:
                     591.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIVLVDMPWSAIDAPSLALGILKRRVLDVFPDARIDVVHANLDY
                     VDWLTARTGFTFEEYDFCASSYFSGHSEWIFSAALNEQPGWHPEFGGSLGSRVPEPLLA
                     KARELHRLAPLFVGETAARITAGGPDVVGFTTTFAQNAAALATARAVKVRAPRAVTVFG
                     GANCDGPQGAALHRNFPFVDFVVRGEGEAAFPQLLAALRDGGEFAGISGLCWRDTGGRC
                     VTNPMEAKPLPPAALVTPEYGDYFDRHARSTAGSWVEPRIVVEGSRGCWWGEKHHCTFC
                     GLNGSFMEFRSKNPQRFVDEILGLVERYRILDVWVTDNILDMAYLTSMAPRLQESGYDL
                     RLCYEIKSNLRREQLRTLSAAGIGYIQAGVENLSSRVLKLMDKGVTGCQNVRLLRDAET
                     VGMNLNWNYLYGFPGESEEDYDEVCDQVPALHHLTPPFNATRLKVERFSPYFDRPELGF
                     DNLRPASHYHHIYDLPDSELADLVYLFDSPKRGIPPSCLDRLKQAVSGWKDAYVHCRLT
                     HCDLGDHIVLVDTRPGFTWHLLDLTDPLETTVFRLLDMPHSPASLLRKAAARHADATEN
                     RIGELLARWRRLGLVFTDGGQYIHIAPAVANQDLMRLDGSHLHSAEPLAPALAAAGGIR
                     "
     gene            complement(6970..7797)
                     /locus_tag="A6A06_RS21580"
                     /old_locus_tag="A6A06_14790"
     CDS             complement(6970..7797)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6A06_RS21580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6A06_14790"
                     /product="hypothetical protein"
                     /protein_id="WP_199931044.1"
                     /transl_table=11
                     /translation="MRAPPGQQLNGQQLTDLLMRLLLDAPFRQRLAVEGAAAVAAGAGE
                     LECLETVDLAELDSAARQFRTAIWKPGRGGGISAAFPRSLRILAAAGVGDAELLTGFLR
                     SEEFGRFRLIPHTGRGLSVEEAFASYVLGFVAAYGERLTRAGAEAAEAVDAARAPVVLR
                     ETVTHELMTALFTALVREQPLSFDIAAEGIVTTGRGHAALRRYTPRSVASWADRPTAAA
                     RPERGEPVAYAYFATPAGVTRGAVSARITAAFETTPSAEGDAARHALSGRGLW"
     gene            complement(7794..8795)
                     /locus_tag="A6A06_RS21585"
                     /old_locus_tag="A6A06_14795"
     CDS             complement(7794..8795)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374907.1"
                     /locus_tag="A6A06_RS21585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14795"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_073762902.1"
                     /transl_table=11
                     /translation="MTTPSPTAARTTGSGAAHRLGLGVLVDMPWSAEGVGGRGAGFVDT
                     DDGGDIHPRLRAFFRRHGGDFGYAAHSFQPRSRALPRAVDYVRAYDRFGQALPAGLPRA
                     FHHTELNMGTPERYDRRPAAEFANELHARYGFAWVVEDLGIWSLRGCPLPYPLPPPLTG
                     PGLEIATRNAAEAARLLVPPLHVEFPGFTEGGSFALGGMDAFEYFAGLADGADVWVTLD
                     VGHLLSYQWLRGRTGPYMYDGLEALPLRRCRELHLSGCQIVNGRFRDLHHGVLLDEQIA
                     LTTCLLDQCPNLVGVTYEDPVFREDGRLVEKSRRNYDRLRELVAEWQGQGPL"
     gene            complement(8792..10810)
                     /locus_tag="A6A06_RS21590"
                     /old_locus_tag="A6A06_14800"
     CDS             complement(8792..10810)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="A6A06_RS21590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_14800"
                     /product="radical SAM protein"
                     /protein_id="WP_073762903.1"
                     /transl_table=11
                     /translation="MPRVPAVDGEPVDVLLLFPPQTEARFFPYLSLPYLTGHLRRRGRS
                     VHQADLNIALLHEILRDPRLLDAPARARGGDGMSDWYRREMARAVDRHIHEIRAHVMRK
                     TSPGEFGAARSVRLARQALELFVRDTFLTSAWHGLDELDSAVLRAADRPPAASGPAVEV
                     LHRLVNELLTRHPPRVVGLSVAFFSQLGPALLIAAWVRRLSPRIKICLGGQQIMLREAN
                     LSVLPGLRRTVDALCWTAGEEPLERWLDALDGTIPSTEVPGMTWSPRAGNAAPAGNTAP
                     PRRAPTLRFHEFGPPDHIGLPVRSYLNEGLEVAIVSCVGCYWGRCVFCAYGNRSLPPGR
                     YQQATVGQVADAVETVVRDTGTRFVAIVDENTNLRLVSKAMRAVRERGVRVRFGVRSRL
                     ESSLTDPAFCRALAEAGCAMIAVGYEGNSQRLLDLMDRGVRAADYQRIVDNVTGAGITL
                     RFSVMGHVLDERPEEFEESLRFLAANERHLGIDALELMVPEPGSRLAQDPERFGVSLDA
                     SGRLAGNPELSYLGGRVGYPLTVHGGPSRTEALARLERVFQVVRPGRVSAVHPPSRRAA
                     DRGGRRSADAARPHPWVRTVPARLDAVPDRIVVADLVWERFYALPCRDVEQHPDGLLTA
                     RTARGQRLLARLVDVSAAEPCRAVPAAAVAPHIRRPT"
     gene            complement(10947..11099)
                     /locus_tag="A6A06_RS41870"
     CDS             complement(10947..11099)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6A06_RS41870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_199931045.1"
                     /transl_table=11
                     /translation="MTALRPEVPVEEEFPELDELEVIDSKELKLGGSYHAYVYIAAEDV
                     FETAD"
     misc_feature    11135..11150
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.52"
                     /tool="antismash"
ORIGIN
        1 tactggccga tccagcggtc ggcgaggtcg tggatgggta cgggattgag gtagtgcagc
       61 ctttcccggc cgtgccggac cgccgtgacc aggcccgcgc cctccagcac cgccaggtgc
      121 ttggacaccg cctgcctgga catctccagc cccgcgcaca gctcgcgcag gctctggccg
      181 ttccgctcgt tcagccggtc cagcagccgc ctgcggttcg cgtccgcgag cgccttgaac
      241 acctcgtcca tgccatccaa ccccctcgtc atcagattgg caaccatttg gttgcgtgtc
      301 aagccgggca gaccccgccg accgggccgg accgcccacg ggaccggcct ccccgagatc
      361 agccgggctc ggcctgacct gcgaggacgc ccgccccagt ggctgtcttc ccggcgggcc
      421 gccgcagggc ggacgcgagc cggaccaagg tggcgccgac cacgatgtgc agcgtgctgg
      481 tcagcgcgga gtcgggcagg gcggtcagga acgcgaagag gggcagcgcc gtcgcgtacg
      541 accatgacgc gccccgcgga aacacccggg cacggatcat cgccgccccg aagagggcgc
      601 agcccacggc gaagacggcc gcggaaccga tgaacgcggg gaccgtgggg cccgtaaggg
      661 tcttctcgac ggtcccatcg tcgaagtaga acagcacgag gttcgaggcg aacgcggcgc
      721 cgccgaacag gccgagcccg atgaggttga cgacgtaggc cgtctccccg aagctgccgg
      781 tgaccgggcc ctggcgccgg tgcagcccgg ccagcagcgg cagcgcgaac gcgggggaca
      841 gggcgaggaa gaagctggtc gccgcggtct ccccggtgaa gatctcgacg agtgcgggca
      901 gggcgacgag gagtccggac agcgcgccgg acagcgcgtc gatgcaggtg gaccggtcgg
      961 acgagggtat gggcacgacg gcactccaat acgtcgaagg acggccccgg cccggaaccg
     1021 cgcgggccgg gaccgagaac gttagggagc cgccggccct tcgcgtcagg tgcagaacgg
     1081 cgagaccgac cgccggtggt caccaggctc accgctgccg tacggccatt cggcacgtat
     1141 cgcctgaccg catccgcctg tggccgcccc gtgccctcgc ctcccggccg ccgcccgcct
     1201 agcctgggcg gcacgcagga gggggtgggc gtgtggcagc gtacgtaccg gtgccgtggg
     1261 tatcgcccct gttgtacgcc gcggtgctgg tctgcggcct ctactacgcg gtggcggacc
     1321 tcacccccgg acccggcccg ccgatctggc gcacggcggg gttcgccgcg gggctcgtgg
     1381 cgctggtcgc cttggaggtc gtggagcggc gcggggtcgt cgcccgcgcg tgcttgccac
     1441 tgctcgcgcg gggcagcctg atcgcggtgg tggtcgtcct cgacgagtcg gggctggccc
     1501 aggccctttt cgtgctcctg ccgttcaccg cctacttcgt cttcggacgc tgcgccgcac
     1561 tcgcgctggc ggctctgtgc gtggccgggc tcctcatcgg atacgcgctg accgagcccg
     1621 gctggtacag cgaagcggag cgcgtgtccg acctgctgat gctctgcgtc ggcctcatcc
     1681 tcgcgctctc catggcggcc gtcgccatcg gggaacagcg agcacggcgc gtcctggaga
     1741 agtacgccgc gcaggtcgcg gaactgtccg ccacggccga gcgcaaccgc ctcgcgcggg
     1801 acatccacga cagcctcgga caccacctca ccgccgtctc cgtacagttg gagatcgcct
     1861 ccgagttccg ggccctggaa cccgactccg cccagcgagc ggtcgacgag gcgcggctgt
     1921 cggtgaagcg cgcgctgggc gacgtacggc agtccgtgcg ggccctgcgg ggcgaggaga
     1981 cgcacaagtc gctgacggtg gggctcgcgg gcctggcgtg cgacgacgag gcccggcccc
     2041 acgtcaccgt gcaggtggac ggatccgagg acggctacgg cccggcggag ctgaccgcgc
     2101 tctaccgggc cgcacaggaa ggcctgacca acgcgcgccg tcacgcgcgg gccacgcggg
     2161 tgacggtggc ggtccgcctg accgaacacg cggcacgcct cgtggtgtcc gacgacggct
     2221 gcggattcgt cccgaacggg tccgtcaccg ggttcgggct gctggggatg cgggagcggg
     2281 tgcacatggt gggcgggagc gtgcacatcg acagcggtcc gggcgcggga acccggctga
     2341 cggtgacggt gccgcgagcc cacagtgagg aaggcgtgcg gtgagcgagg gggcgacgcc
     2401 ggtacgcgta ctcgtcgtgg acgaccagcg gctcatccgg gacggcatcg cgtcgctgct
     2461 ctccatccgg ccgggcatca cggtcgtcgg cacggcggtg gacggccgcg aagccgtcgc
     2521 gaaggtgctc gaactccgcc cggacatcgt gctgatggat gtccggatgc cggggatgga
     2581 cggcgtcgag gcggtcgccg tgctgcgcgg cgaggcgccg gagtgccgga cggtgatgct
     2641 gacgacgttc gacgacgagg agtacgtcgt ccaggccatc cgcgcggggg cctgcggcta
     2701 cctgctcaag gacctgccgg ccgacgaact cgcccacgcg gtccggctcg cgtacgcggg
     2761 cgtcacccag ctcgacgcct ccgtgacccg ccacctcgcc gcctgcctgc cctgtccggg
     2821 ccccggagcg gagccggcgg tcgccctcag cccgcgcgag atcgacatcc tgcgcctcgt
     2881 ggcgcaaggc aggaccaacc gggagatcgc cgcgcagctg tacctcagcg agggcaccgt
     2941 gaagaactac gtctcccgca tcctcggccg cttcgccttg cgcgaccgca cccaggccgc
     3001 actgcgggct cgggaactgg gcctgctgtg agcgcgagcc cgacgaggag cggggccggt
     3061 accggggttt tcagggggtt tcgctccgtc gaagcgtggc agggagccgc cagcaagaaa
     3121 ggggagggac catgggcgga atcgatccgc acatccatgt cgacgcgaag gtggtccacg
     3181 gcgatgccgc tgcccgcaac ctgttggcat ccacgttcgg cctggtcggt aacgtgccga
     3241 gcacggtcag caccggatgc gggctgcggg tgccctacgc gatggcctct ccgcgtccgg
     3301 accgcgtcac ctgccttgcc tgccgtgagc acgcgcggcg cgagcacctg cgcctcgcgg
     3361 agcaggtcga acgcttgagc cgcatgctgg gatcggccat cagccctgcc catggcaagg
     3421 cggtcgcgga ctggcatcgc gacctcgcgc agaagttttc cgacgcggag agttgaaccg
     3481 gtaaccgggg caacggacgc gactcgacac gggagcgtcg gatgggcgag agggcacacg
     3541 gacccgcaca tcgcggagcg ggcagcggtc caggggcgtt cacgccggac gggaacgcgg
     3601 tggagctgta cgcccggctt cccgccggag ccgagccgga gatcattgcg cgggtcgtgc
     3661 cgccgggggc ctcgatactc gaactgggcg cgggagccgg gcgggtgacc cacgtactgc
     3721 tggagcgtgg ctatcgagtc gtcgccgtgg acgagtcgga ggagatgctc agccgggtca
     3781 agggggctcg tacggtgcat ggctccatcg aggaactgga cctcggcgag cagttcgatg
     3841 ccgtgctgct cgcctcccat ctgctcaaca ccgccgagcc gccctcgtgc gacgcgatgt
     3901 tgcgaagttg ccgccgtcac gtggccgccg ggggacgggt gttggcggag cggcagggcg
     3961 aggactggtt caggccccgc agggcaccgg ccggcgggga acgggcgggg gcccgcatgc
     4021 ggatcgtttc cgtggaccgc ccgatgcccg gtgtctccga cgtacgtatc gagtacgaga
     4081 tcgacggcac gcggtggacc cactccttcc gtgcccggga tgtcccggac gcgacgctgg
     4141 agcgcatgct gacggccgcc gggctccgac tggtccggta tctcaccgag gaccacagct
     4201 gggtggaagc ggttcccgtc tgaacgcccc gtccgcctac ggaatggtgg tgctcagcgg
     4261 ccagcggcgg atccggtacg gcgtccacca caggaagcgc cccgcccggt ggatcaggcc
     4321 gggtggcagg tacctcgcgg tgacggaagg cggcacgccg gtctccggca ggccgcgcag
     4381 cagggactgg atcagctggt cgtgctggta cgtgcggatg acgaggcgct tgaacgaacc
     4441 gccgcggcgg tcccggacct cgatgaagcc ggggccgcgg cggtatccgc acatcgcggc
     4501 gtggaacgag tcgcgccacc ggacggcgat gtcttcgccg gagccgtccg agccgccgga
     4561 gccgtccgag ccgccagaac cgtccgagct gccggagccg ccacggaaca cggcggtggg
     4621 agggtggagg tggctgagcg gctgccactc gaccggtgtg ccggttccga ggcccatgcc
     4681 ggtgccggtt ccggtgccgg ttccgggctc gggctcgggc agacgcagcg tccagtcgac
     4741 ggcgatgccg aaggcggtga gttcgcggac ggcgacgagg gccgtcaccg cggcggcgcc
     4801 gtcgcgatcc gtcagatcga cggtccgggg aaggcgggcg aactggacgc ggtgggcggc
     4861 gaggtccgcc accgtgctca cctggtcgcc gatcaggtcg agcgtgccga cgtacacccc
     4921 cgggatgtcc ctggccgccg ggtcgcgttc cctccacagt gccagggtca ccggggcggg
     4981 gggccgggtg gcgagcgggc tcaccggatt cctccggcgg cggcgagggc cggggcgagg
     5041 ggctcggcgc tgtgcagatg gctgccgtcc aggcgcatca ggtcctggtt ggcgaccgcc
     5101 ggggcgatgt ggatgtactg gccgccgtcg gtgaagacga ggccgaggcg gcgccagcgg
     5161 gcgaggagct caccaatccg gttctcggtt gcgtccgcgt ggcgggcggc ggccttgcgc
     5221 agcagggacg cgggggagtg cggcatgtcg agaaggcgga agacggtcgt ctccaggggg
     5281 tccgtcaggt ccaggaggtg ccaggtgaac ccggggcggg tgtcgacgag gacgatgtgg
     5341 tcgccgaggt cgcagtgggt cagacggcag tgcacatagg cgtccttcca gccgctcacg
     5401 gcctgcttca ggcgatccag gcaggacggg gggatgccgc gtttggggga gtcgaagagg
     5461 tagaccaggt cggcgagttc ggagtcgggc aagtcgtaga tgtggtggta gtgggaggcg
     5521 gggcggaggt tgtcgaagcc gagttctggc cggtcgaagt aggggctgaa gcgttcgacc
     5581 ttgaggcggg tggcgttgaa gggcggggtg aggtgatgca gggcgggaac ctggtcgcag
     5641 acctcgtcgt agtcctcctc cgactcgccg gggaagccgt agaggtagtt ccagttcagg
     5701 ttcatgccga cggtctccgc gtcgcgcagc aggcggacgt tctggcagcc ggtcacgccc
     5761 ttgtccatga gtttcagcac ccggctgctg aggttctcga caccggcctg gatgtagccg
     5821 atgcccgccg cgctgagggt ccggagctgc tcgcgccgca ggttcgactt gatctcgtag
     5881 cacagccgca ggtcgtagcc ggattcctgg agccggggcg ccatcgaggt gaggtaggcc
     5941 atgtcgagga tgttgtcggt gacccacacg tcgaggatgc ggtaccgctc gaccaggccg
     6001 aggatctcgt cgacgaaccg ctgcgggttc ttgctgcgga attccatgaa cgagccgttg
     6061 agcccgcaga acgtgcagtg gtgcttctcg ccccaccagc acccgcgtga gccctcgacg
     6121 acgatgcggg gctccaccca gctgcccgcg gtggaccgtg cgtggcggtc gaagtagtcg
     6181 ccgtactcgg gtgtgaccag cgccgccgga ggcaggggtt tggcctccat ggggttggtg
     6241 acgcagcggc cgccggtgtc ccgccagcac aggccggaga tccccgcgaa ctcgccgccg
     6301 tcccggagcg cggccagcag ctgggggaac gccgcctcgc cctcaccccg tacgacgaag
     6361 tcgacgaacg ggaagttccg gtgcagtgcc gcgccctgcg ggccgtcgca gttcgcgccg
     6421 ccgaaaacgg tcacggcgcg cggagcgcgg accttgaccg cgcgagcggt ggccagagct
     6481 gcggcgttct gtgcgaaggt cgtggtgaaa ccgaccacat cgggcccacc ggcggtgatc
     6541 cgggcggcgg tctcgcccac gaacagcggg gcgagtcggt gcagttcccg tgccttggcg
     6601 agcagcggct cgggtacccg ggaccccagt gacccgccga actccgggtg ccacccgggc
     6661 tgctcgttga gggcggccga gaagatccac tcgctgtgtc cggagaagta cgagctcgcg
     6721 cagaagtcgt actcctcgaa cgtgaagccg gtacgtgccg tcagccagtc gacgtagtcg
     6781 agattggcgt gcacgacgtc gatccgggca tccgggaaga cgtcgagaac ccggcgcttg
     6841 aggatgccga gggccagcga cggggcgtcg atcgccgacc acggcatgtc caccaacacg
     6901 atgcgcatga cagtccgttc cctttctcgc cgaggccgtc cggcgggcgc gccggtgtgg
     6961 gcggcggcct caccagagcc cgcgcccgga cagcgcgtgg cgggcggcgt cgccctcggc
     7021 cgatggggtg gtctcgaacg ccgcggtgat ccgcgcggag acggctccgc gcgtgacccc
     7081 ggcgggtgtg gcgaagtacg cgtacgccac cggttccccg cgctccggcc gggcggcggc
     7141 cgtcgggcgg tccgcccagg aggcgacgga gcggggcgtg taccggcgca gggccgcatg
     7201 gccccggccg gtcgtgacga tcccctcggc ggcgatgtcg aacgacagcg gctgttcccg
     7261 gaccagggcc gtgaacaggg ccgtcatcag ttcgtgcgtc acggtctcgc gcagcacgac
     7321 gggggctcgc gctgcgtcca ctgcttccgc cgcttccgcg cccgccctcg tgagccgctc
     7381 cccgtacgcg gcgacgaagc ccagcacgta cgaggcgaac gcttcctcca cggacagtcc
     7441 gcgcccggtg tggggaatca gccggaacct gccgaactct tcggagcgga ggaacccggt
     7501 gagcagttcc gcgtccccga cgcccgccgc cgcgaggatg cgcaggctgc gcgggaacgc
     7561 ggcggagatg ccgccgcccc tgcccggctt ccagatcgcg gtgcggaact ggcgagcggc
     7621 actgtcgagt tcggcgaggt cgaccgtttc caggcactcc agctcaccgg cccccgcggc
     7681 gaccgccgcg gcgccttcga cggccaggcg ctgcctgaaa ggggcgtcca gcagcagccg
     7741 catcagcagg tcggtgagct gctgaccgtt caactgctgt cctggaggcg ccctcatagg
     7801 ggcccctgtc cctgccactc ggcgaccagc tccctgagcc ggtcgtaatt gcgccgggac
     7861 ttctccacca gccggccgtc ctcacggaac accggatctt cgtaggtgac gccgacgagg
     7921 ttcggacatt ggtccagcag acaggtggtg agagcgatct gctcgtcgag gagcacgccg
     7981 tggtgcagat cgcggaacct cccgttgacg atctggcagc ccgacaggtg gagttcgcgg
     8041 cagcggcgca gcgggagcgc ctccagaccg tcgtacatgt acgggccggt gcgtccgcgc
     8101 agccactggt aactgagcag gtggccgacg tcgagcgtca cccacacgtc ggcgccgtcg
     8161 gccagcccgg cgaagtactc gaacgcgtcc atgccgccca gcgcgaacga gccgccctcg
     8221 gtgaaaccgg gaaactcgac atgcagcggc ggcaccagga gccgtgcggc ctcggccgcg
     8281 ttgcgggtgg cgatctccag gccggggccg gtcagcggcg gcggcagcgg atacggcagc
     8341 ggacacccgc gcaggctcca gatccccagg tcctccacca cccaggcgaa gccgtaccgg
     8401 gcgtggagct cgttggcgaa ctcggcggcc ggccggcggt cgtagcgctc gggcgtgccc
     8461 atgttcagtt cggtgtggtg gaaggcgcgg ggaaggccgg caggcagcgc ctgcccgaac
     8521 cggtcgtagg cgcggacgta gtcgacggcc cgggggaggg cacgcgagcg tggctggaag
     8581 gagtgggccg catagccgaa gtcaccgccg tgacgccgga agaaggccct cagccggggg
     8641 tggatgtcgc caccgtcgtc cgtgtccacg aacccggcgc cccgcccgcc gacgccctct
     8701 gcggaccacg gcatgtccac gaggacaccg agacccagcc ggtgcgcagc cccgctgccc
     8761 gtggtgcgcg cggcggtggg agagggcgtg gtcacgtcgg cctcctgatg tgcggggcga
     8821 ccgcggctgc gggcaccgcc cggcaaggct cggcggccga cacgtccacg aggcgcgcca
     8881 gcagccgctg tccacgggcg gtgcgggccg tgagcagacc gtcggggtgc tgttcgacgt
     8941 cacggcacgg caacgcgtag aaccgttccc acaccagatc ggccaccacg atgcggtcgg
     9001 gcaccgcgtc gaggcgggcg ggcacggtac gtacccacgg atgcggcctc gcggcgtcgg
     9061 ccgaacgccg gccgccccgg tccgccgcac ggcgcgacgg cgggtgcacg gcgctcaccc
     9121 ggccgggccg caccacctgg aagacccgct ccagccgggc gagggcctcg gtgcgcgacg
     9181 gaccgccgtg caccgtcagc gggtagccga cccggccgcc gaggtagctc agttcggggt
     9241 ttccggccag ccggcccgag gcgtcgagcg agaccccgaa acgctccggg tcctgtgcga
     9301 gcctgctgcc cggttcgggc accatgagtt cgagcgcgtc gatgccgagg tgccgctcgt
     9361 tggcggccag gaatcgcagg gactcctcga actcctccgg acgctcgtcc aggacatggc
     9421 ccatcaccga gaaccgcagg gtgattcccg ccccggtgac gttgtccacg atccgctggt
     9481 agtcggcggc gcgcacgccc cggtccatca ggtcgagcag gcgctgcgag ttcccctcgt
     9541 accctacggc gatcatggcg cagcccgctt ccgccagcgc ccggcagaac gccggatcgg
     9601 tcaggctgga ctccagccgg ctgcgcaccc cgaaccgcac ccgcaccccg cgctcgcgca
     9661 cggcgcgcat cgccttggag accaggcgca ggttggtgtt ctcgtccacg atcgccacga
     9721 accgtgtccc cgtgtcccgg accaccgtct cgaccgcgtc ggcgacctgc cccacggtgg
     9781 cctgctggta cctgccgggc ggcagcgacc ggttgccgta cgcgcagaac acgcaccgcc
     9841 cccagtagca gccgacgcag gagacgatcg ccacctccag gccctcgttg aggtacgagc
     9901 ggaccggcag gccgatgtgg tcgggagggc cgaattcgtg gaaacgcagg gtcggtgccc
     9961 ggcggggagg ggcggtgttc cccgcggggg cggcgttccc cgcgcgcggc gaccaggtca
    10021 tgccgggcac ctcggtcgag gggatcgttc cgtcgagggc gtcgagccag cgttcgagtg
    10081 gttcctcccc ggcggtccag cacagggcgt cgacggtacg gcggaggccg ggcaggaccg
    10141 agaggttcgc ttcgcggagc atgatctgct gtccgcccag gcagatcttg atgcgcgggc
    10201 tgagtctgcg cacccaggcg gcgatcagga gggcgggtcc gagctggctg aagaacgcca
    10261 ccgagagccc gaccacgcgc ggcggatgcc gggtgagcag ttcgttcacg agccggtgga
    10321 gcacctccac cgccggtccg gacgcggcgg gcggccggtc cgccgcccgc agcaccgcac
    10381 tgtccagctc gtcgaggccg tgccaggccg atgtcaggaa ggtgtcccgc acgaacagtt
    10441 cgagtgcctg ccgggcgagc cgcaccgagc gtgcggcgcc gaactcgccc ggggacgtct
    10501 tccgcatgac atgtgcgcgg atctcgtgga tatgccggtc gacggcacgg gccatttccc
    10561 ggcggtacca gtcgctcata ccgtcgccgc cacgggcacg cgcaggggcg tccagcaggc
    10621 gtggatcgcg cagtatttcg tgaagcaggg cgatgttgag gtcggcctgg tgaacgcttc
    10681 ggccccggcg ccgcaggtgg ccggtgagat agggcaggct cagatacgga aagaacctgg
    10741 cttcggtctg cggcgggaag aggaggagta cgtcgacggg ctcaccgtcc accgccggta
    10801 cgcgcggcac ggcccacctc cttgcccgcc ggacggccgt acaccacggc agcacggcgc
    10861 gctctgcgtg aatgcgtaac gcagcgcgga acccccggga acgacggagg cggaaccgca
    10921 cgcagcccgc ggaaccggta cgggactcag tcggcggtct cgaaaacgtc ctccgcggca
    10981 atatagacgt aagcgtggta ggagccgccg agcttcagct ccttggaatc aatcacttcc
    11041 agttcgtcga gttccgggaa ttcttcctcg acgggcactt cggggcgcag agcggtcatg
    11101 acccctccct gatgggaaca tggcgggttg tcgaatggaa gatcagtttc gcgctgattt
    11161 cttgattccg agccgcgccg cgcatgcgcg gccggagcgg ctcccggccc acggtacttc
    11221 cgggccaccg gaggtcacaa cgccggtgga gtcgcgaatt accttcctgt ggcacgcatt
    11281 cggctccgtg cggtgtggtg ctgtgcgatg cggtgtggcg cggttccgag gtgctgtcct
    11341 tgaccgtcag gaaagaacaa gaggaacatc tcgcaatggg aaagccacgg gacatgaagc
    11401 ccatgacggc acaggaatgg aacctgacct atcaattcgg cgacccggtg tgggacgagc
    11461 cgcccaccgc accccttgcc cgcctgctcg cccgtcacgc cccgcctccc gcagaggtga
    11521 tcgacgtcgg atgcgggctg ggtgccgcct cgcgctggct cgccgagcac ggctaccagg
    11581 tcaccgggtg cgatttctcg tcacgggcga tcgaactggc ccgtgcacgg acccccgcgg
    11641 cgctgccggt ccggttcacg atggccgacg tcatgcacgt accaccgcgg ctcggccggt
    11701 ttccggtcgt actggagcgg ggcgtcctgc atgccgtacc gcacacgccg gacaggcgtc
    11761 acttcgtcga ggcgatggca cgtgtctgcc tcccgggggg cctctggatc cacgtgggcg
    11821 cgagcgccgc caccgtggag gcagaacaca gcgtggcacg aggcccctcc cgcatggccg
    11881 aacagacctt tctcgccctg gtcgaccc
//
