LOCUS       NZ_LWKZ01000008        10984 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold16, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000008
VERSION     NZ_LWKZ01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 81883)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81883)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 59539
            Orig. end                         :: 70523
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    164..177
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.79"
                     /tool="antismash"
     gene            898..2544
                     /locus_tag="A6A06_RS10760"
                     /old_locus_tag="A6A06_37690"
     CDS             898..2544
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984546.1"
                     /locus_tag="A6A06_RS10760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37690"
                     /product="hypothetical protein"
                     /protein_id="WP_073760988.1"
                     /transl_table=11
                     /translation="MILSEEYLQDLFDKTFPQISSAAGCAVVLEGSIAEGFGNSSSDID
                     FLLIADSDADLPTMPSLLFVDGRRVEIRTRSVRQIEEQFSAVTSDRPGTAAEDLLNRCQ
                     RLLRSYPLRNPELVAKVKGLMSYDDFQTTARDWWAHAARQSIRYALTLRQLGQEQEAAA
                     WTEAGLLQAVKSWAAGLGETYLEPKWLPEQLKRIGDHPLAARYLTLASLDASGLDAAEY
                     VAAGARLTADLGVTGAEPGPGQITVAGAGAVTTWQTGERVHVVRGRQDVFVLGDRAARA
                     WRSLVFGRSLEQVLAAADAAGAPQAGPLIAQFMRFGLVRAAWQGAGPIIPSMPLAAPAG
                     DVTPPPSTARPVVVVGGAAVGGEEAVDLVPVPARRFSAAAMTLVWSNVLVENAREDLVG
                     ALDRAQWSVAQLSARRMVRFALRGVLSAHAVNPLPPDSEVVRRLSLLPAGADTDEIRAG
                     AAELEVLTIASDDEGAAALAALDGFVALVRRATGADSFPSSFDSADGWRQTLEIGYDWL
                     RLGAHLNADLPIDEASDLLSSGGAQPHAASA"
     misc_feature    complement(936..947)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.3"
                     /tool="antismash"
     gene            2615..2848
                     /locus_tag="A6A06_RS10765"
                     /old_locus_tag="A6A06_37695"
     CDS             2615..2848
                     /NRPS_PKS="Domain: PP-binding (4-67). E-value: 4.3e-09.
                     Score: 28.6. Matches aSDomain:
                     nrpspksdomains_A6A06_RS10765_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="A6A06_RS10765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37695"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_199930808.1"
                     /sec_met_domain="PP-binding (E-value: 6.2e-10, bitscore:
                     31.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYAIVGAMAPAPDAELADDTRLIADLAYDSLRLLELTLALEQGFG
                     LPEIAPEETATVTTLGDVVRMVTAAQQQEAVL"
     aSDomain        2627..2815
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A06_RS10765_PP-binding.1"
                     /evalue="4.30E-09"
                     /label="A6A06_RS10765_PP-binding.1"
                     /locus_tag="A6A06_RS10765"
                     /protein_end="67"
                     /protein_start="4"
                     /score="28.6"
                     /tool="antismash"
                     /translation="VGAMAPAPDAELADDTRLIADLAYDSLRLLELTLALEQGFGLPEI
                     APEETATVTTLGDVVRMV"
     misc_feature    2843..2845
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2845..3981
                     /locus_tag="A6A06_RS10770"
                     /old_locus_tag="A6A06_37700"
     CDS             2845..3981
                     /NRPS_PKS="Domain: NAD_binding_4 (8-232). E-value: 3.8e-48.
                     Score: 155.7. Matches aSDomain:
                     nrpspksdomains_A6A06_RS10770_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608202.1"
                     /locus_tag="A6A06_RS10770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37700"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_073760989.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2e-19, bitscore:
                     62.0, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 5e-48, bitscore:
                     155.7, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQRLKVLVTGAAGLVGAEVTARLVAAGHEVTAVVHRNSALVRND
                     GTPLDMSSVTCLPGDVTKARLGLSEAGYGELAAGRIDRIVHCAAITDFGRPEEVYQSVN
                     VDGTAHIVELARDAGADLVHVGTAYVCGERDGLVRETELDQGQRFANGYEASKLRAETL
                     VHKARADGVRAAVVRPSIVVGDEHTGVVRDYKNIYIVLKLTTEGKVRSIPGLYEARLDL
                     VPVGYVADVITQVTERFDEAAGRTFHAVGSPLSLRDFSDVMAEYPSFRVPRFVPPTSFD
                     AQRLEPQERRYYERVVSLYESYFRRRMTFDDSNTEALGVRRAPGDGQDFLRRLINHCLE
                     SGYLGTPLAGVTDVLNRLDITGPDTTGPAANDSNGAGR"
     aSDomain        2869..3540
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A06_RS10770_NAD_binding_4.1"
                     /evalue="3.80E-48"
                     /label="A6A06_RS10770_NAD_binding_4.1"
                     /locus_tag="A6A06_RS10770"
                     /protein_end="232"
                     /protein_start="8"
                     /score="155.7"
                     /tool="antismash"
                     /translation="VTGAAGLVGAEVTARLVAAGHEVTAVVHRNSALVRNDGTPLDMSS
                     VTCLPGDVTKARLGLSEAGYGELAAGRIDRIVHCAAITDFGRPEEVYQSVNVDGTAHIV
                     ELARDAGADLVHVGTAYVCGERDGLVRETELDQGQRFANGYEASKLRAETLVHKARADG
                     VRAAVVRPSIVVGDEHTGVVRDYKNIYIVLKLTTEGKVRSIPGLYEARLDLVPVGYVAD
                     VI"
     gene            3978..4988
                     /locus_tag="A6A06_RS10775"
                     /old_locus_tag="A6A06_37705"
     CDS             3978..4988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984548.1"
                     /locus_tag="A6A06_RS10775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37705"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_073760990.1"
                     /transl_table=11
                     /translation="MTRDSADARPFGDSVSPALSCEPRKRALFASDTYFLESLEQLGAL
                     TADVDGFLHRHAALLLKPDAVVSRQIPTTVAWLAENGFRIVAAERTRLTRTAVRSLWYY
                     QWNLATPQRRRLADMFMDSCDAVVLVVRPVADEADAAPASVVMTQRKGPTDPLARVPGQ
                     LRYAIGRYSYLLNLVHTPDEPADVARELGIHFDTALRERVYTSALAGDDQSARALELAE
                     QLHAEVPQRDLTFEPAAERLKQAVEEAEESLLGSPARDELREARLAARSPEGYRRLLEA
                     AWRAGLPLDTWDVVIVGTHVLPMKRKDLAPVLDGVGVQDWQLHMARLEADRSAAR"
     gene            5001..5984
                     /locus_tag="A6A06_RS10780"
                     /old_locus_tag="A6A06_37710"
     CDS             5001..5984
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984549.1"
                     /locus_tag="A6A06_RS10780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37710"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_073760991.1"
                     /sec_met_domain="AfsA (E-value: 7.9e-20, bitscore: 63.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTVEFSRTVPRELVHRSSVTEVLVTGALARDDDTLDVGVQIPRS
                     HAFYRDSQGCRHLYDPMVLVEAARQTLIMLGHDVFGMPFGTKFVLREIALHETDMAALT
                     VRGVPTEVVLRCHVLRRFGDHTAVRGARLRYSALIGDHVAATLECSMSWMPPEQWQAMR
                     DQGRIDLGLEPQLVPEAPAPLPRVHRTLVDRQDPSNVVLSSFQVAPGTVARGTAQLVVD
                     MGHPVLFDHYVDHVPGMLTLESFRQLGMATAVATGVLPSASALLTGVSARFTGIGELDL
                     PVTCESVLAQGDGSSAVLHCTMRQDGRSLAEGEVRLTRPGAEMGTN"
     gene            5981..6619
                     /locus_tag="A6A06_RS10785"
                     /old_locus_tag="A6A06_37715"
     CDS             5981..6619
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984551.1"
                     /locus_tag="A6A06_RS10785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37715"
                     /product="nitroreductase family protein"
                     /protein_id="WP_073760992.1"
                     /sec_met_domain="PF00881 (E-value: 8.7e-26, bitscore: 83.3,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSTPVTLDIDGLLSRTPTVRRRLDLSRPVPRPLLAECLQLALHA
                     PNGGNRQSWHFVVIDDPATKAGIARYYKSASDAYLATTSSAAMSEKAVAGVRYLADHLH
                     QVPALVLAGIRGRLPRDAPLAKQSSFFGSVYPALWSFMLAARSRELGAALTTVHLAHER
                     EVAELVGIPADEFTQVALVAVGYTTGGSGFRPTPRQPLDEVVSWNGWQS"
     gene            6607..7524
                     /locus_tag="A6A06_RS10790"
                     /old_locus_tag="A6A06_37720"
     CDS             6607..7524
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /locus_tag="A6A06_RS10790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37720"
                     /product="UbiA family prenyltransferase"
                     /protein_id="WP_199930809.1"
                     /transl_table=11
                     /translation="MAELNGTLKPSQVPARLGRFVLRMFRPQIYVTYGVLWTVTLEGTS
                     GEIAGPDTPWTPSWATAVRAASVVLALLFVRMVDEQKDLDYDRQHNPDRPLVTGAITVA
                     ELRGAMALIAVLVVVLNLLVSAWSVLLILLGLGYTLLLVALERWFPRVGEGLVTGLLIS
                     YPVQLLLSVYLYVSLLTSGKIEADLRAVPLLAMFACAFLQFEFARKTEWRQDPQARLYS
                     EVFGPRGSAAISLALAVGAVVLGLAVLGRAGLLPAAALLFPALGAWRFLLRKKSSGLML
                     PAMGFVLFFNLLCFYGSTALAIGR"
     gene            7530..8789
                     /locus_tag="A6A06_RS10795"
                     /old_locus_tag="A6A06_37725"
     CDS             7530..8789
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608207.1"
                     /locus_tag="A6A06_RS10795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37725"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_073760994.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.1e-24, bitscore:
                     79.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQPVIIDRVRIFDGESMTGPGSVEFADGVITRVVTDPAPRPPASA
                     EVIDGTGMTLLPGLIDAHTHVYGVESNLELALAFGVTTELDMFMGPPEATRALCELAAE
                     RADLADLRSAGLLASAPGGHPGVGMPDLPTVAGPEQADAFVAARQAEGAHFIKIMLDDG
                     VNHGMSLPSLAPATVTALADAARRRSLLSVAHVSAMWSARLALDSGVDILTHLPLEAPL
                     DEAFVRRAADGGRVVIPTLAILELSAPQSSPATGVGRSWADDPRIADHLPDHARAAIAE
                     GREGLCVEHPVPEHRFGHALSSVARLHRAGVPVLAGTDANHRPGRACPVVHGASLHAEL
                     ALLVEAGLTPAQALTAATSAPAAHFGLGDRGVIAPGRRADLVLVAGDPTEDITATRSIE
                     AVWRGGVRFDREAHRKRVAS"
     gene            8914..9777
                     /locus_tag="A6A06_RS10800"
                     /old_locus_tag="A6A06_37730"
     CDS             8914..9777
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983473.1"
                     /locus_tag="A6A06_RS10800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37730"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_073760995.1"
                     /transl_table=11
                     /translation="MTNAPELQAGVSSTALVVGMARAAESRRPDRLFEDHLADRFLEAA
                     GVEQQAVWNKGQGELFAEAMGDYFALRTRYFDDYFRQAAAAGCRQVVLLAAGLDTRAHR
                     LDWPDGTRLFELDQPDVLAFKESALGDEAPRCRREVIAADLREEWTTVLAERGFRPDQP
                     TAWLVEGVLVYLTEHDADGLLSRISALSAPGSHLAVEHVTRSMVNSDQAQQAAAASPEG
                     VVNLLSSLWKNEMTRPVGDWLAGHGWSSTQESLTDLAQRHGRPVPPAFDPALPGTGRVS
                     LLTAVR"
     misc_feature    complement(10051..10067)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.07"
                     /tool="antismash"
     gene            10172..10534
                     /locus_tag="A6A06_RS10805"
                     /old_locus_tag="A6A06_37735"
     CDS             10172..10534
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986855.1"
                     /locus_tag="A6A06_RS10805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_37735"
                     /product="hypothetical protein"
                     /protein_id="WP_073760996.1"
                     /transl_table=11
                     /translation="MTTTVTSRHPRAPRHAPLSVTIASWAVPFMVVAQYALIAGVPVAI
                     ALVGALRHVRDRAVRLAAVLVAVSYAVPLAVWLIRPDGAQSLSKDMHPGFAGLIVAASA
                     LLLATLYRARRLTPDT"
     misc_feature    10741..10757
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.32"
                     /tool="antismash"
ORIGIN
        1 ccggctcgcc gacctggacc tgtccgcgtg gcgcaacgcc atcgtcggcg ccgagcccgt
       61 ggacccccgg gtgctggagc ggttcgcccg gctcgccgcg cccgccggtt tctccccttc
      121 cgtctacctc ccgtcgtacg gcctcgccga ggcgacgctc gcggtgaccc tgcgtacccg
      181 cggcacgccc acgacgaccg tgcgggtgga cagccgcagt ctgcgtttcg gtgctccggt
      241 cgtcatcgag gcgcaccgcg agttcgcgga ggccgaccgg gccgacgacg gcgaggacgg
      301 ctggatgatc ggctgcgggc gccccggcga cggggtgggc gtgacgatcc tcgacgagga
      361 gggcgcgccc ctgccggacg ggcacctcgg cgagatcgag gtgcacggcc cctcggtcac
      421 gggcggctac cacgccgggc ggaccggctc cacgcggttc gtcgacggcg ctgtcaggac
      481 cgcggacgcc ggcttcttcc atcagggcga actgttcgtg ctcggccgga tgggcgacag
      541 cctcaaggcg cacgggcgca gcgtctatgt ggaggacctg gagtcgaagg tggtctccgc
      601 gacggggatc gcccgggaga agtgcgcggt cgtcggcgtc gagggcgtcg gcggagccac
      661 ggtgacgctg ttcgcggaga aggccgcggg cgcgtgggtg gaggacgcca ccaaggtgtt
      721 gcgcggcgcg ctcgcggacg aggtcgccat cgccgtcgtc gtcggcggca gtgggctgat
      781 cagcaggacg tccagcggta agccgaagcg ccgccacatg tgggaacagc tgcaagcggg
      841 ccgcctgaag aacgcgcgcg ttctggggcc ggccgacgcc acgaccggga ggcccgtgtg
      901 atcctcagcg aggaatacct gcaagacctg ttcgacaaaa cgtttccgca gatctcttcc
      961 gcggccggct gcgccgtggt gctggagggg tcgatcgccg agggcttcgg caactcgtca
     1021 tccgacatcg acttcctcct gatcgccgac agcgacgccg acctgccgac catgccgtcc
     1081 ctgctcttcg tggacggccg gcgcgtcgag atcaggaccc gttccgtacg acagatcgag
     1141 gagcagttct ccgcggtcac gtcggaccgt ccggggacgg cggcggagga tctgctgaac
     1201 cgctgtcagc ggctgctgcg cagctacccg ctgcgcaatc cggagctggt ggcgaaggtc
     1261 aaggggctga tgtcctacga cgacttccag accacggcac gcgactggtg ggcgcacgcc
     1321 gcacgccagt cgatccggta cgcgctcacc ctgcggcagc tcggccagga gcaggaggcc
     1381 gcggcatgga ccgaggccgg gctgctgcag gccgtgaaat cctgggcggc cgggctggga
     1441 gagacctacc tggagcccaa gtggctgccc gagcagctga agcgcatcgg tgaccacccc
     1501 ctggcggccc ggtacctgac cctcgcgtct ctggacgcct ccggtctcga tgccgccgag
     1561 tacgtcgccg cgggcgcccg gctcacggcg gacctgggcg tcacgggcgc cgagcccggt
     1621 cccgggcaga tcaccgtggc cggggccgga gcggtgacca cgtggcagac cggcgagcgc
     1681 gtgcacgtcg tacgcggccg gcaggacgtg ttcgtcctcg gggaccgggc ggcgcgggcc
     1741 tggcgctcac tcgtcttcgg ccggagcctg gagcaggtgc tggccgcggc ggacgcggcg
     1801 ggcgcaccgc aggcgggccc gctgatcgcc cagttcatgc ggttcggcct ggtgagggcg
     1861 gcgtggcagg gcgcgggccc gatcatcccc agcatgccgc tggccgcccc cgcgggtgac
     1921 gtcacaccgc cgccgagcac cgcgcggccc gtcgtcgtcg tgggcggagc ggcggtcggc
     1981 ggcgaggagg ccgtcgacct cgtgccggtg ccggcccgcc ggttcagtgc cgccgcgatg
     2041 accctggtgt ggtccaacgt gctggtggag aacgcccgcg aggacctcgt cggcgccctc
     2101 gaccgtgcgc agtggagcgt cgcccagctc agtgcccgga gaatggtgcg gttcgcgctg
     2161 cggggcgtac tcagcgccca cgccgtcaat ccgctgccgc cggactcgga agtcgtccgc
     2221 cggctgtcgc tccttccggc cggggcggac accgacgaga tccgggcggg cgccgccgag
     2281 ctggaagtcc tcacgatcgc ctccgacgac gagggcgccg ccgcgctggc ggcgctggac
     2341 ggcttcgtcg ccctggtccg ccgtgccacc ggcgcggaca gcttcccgtc ctccttcgac
     2401 tccgcggacg gatggcggca gaccttggaa atcggttacg actggctgcg cctcggcgcg
     2461 cacctcaacg cggacctgcc catcgacgag gcgagcgatc tgctgtccag cggaggggcc
     2521 cagccccacg ccgcttcggc ctgacgctcc tggaggaaca ttgacctcac gtacggatcc
     2581 cccctgcccg accgaggacg acacgcggag caaggtgtac gcgatcgtcg gcgccatggc
     2641 gcccgcgccg gacgccgaac tggccgacga cacccggctg atcgccgatc tggcctatga
     2701 ctcgctccgg ctcctcgaac tgacgctggc actggaacaa ggcttcgggc tgcccgagat
     2761 cgccccggag gaaacggcca cggtcaccac gctcggcgat gtcgtgcgca tggtgaccgc
     2821 ggcacagcaa caggaggcgg ttttatgaca cagcggctga aggttctggt caccggtgcg
     2881 gccgggctgg tcggcgcgga ggtcaccgcc cggctggtgg cggccgggca cgaggtgacc
     2941 gccgtggtcc accggaactc ggcgctggtc cgcaatgacg gcacccccct ggacatgtcc
     3001 agcgtcacgt gcctgcccgg cgacgtgacc aaggcccggc tcggcctgtc cgaggccggg
     3061 tacggggaac tggccgccgg ccggatcgac cggatcgtgc actgcgccgc gatcaccgac
     3121 ttcggccgtc cggaggaggt ctaccagtcg gtcaacgtcg acggcaccgc gcacatcgtg
     3181 gagctggccc gcgacgccgg cgccgacctc gtacacgtgg gcaccgccta tgtgtgcggt
     3241 gagcgcgacg ggctggtccg tgagacggag ctggaccagg gccagcggtt cgccaacggc
     3301 tacgaggcca gcaagctgcg ggccgagacg ctggtgcaca aggcacgggc cgacggggtg
     3361 cgggccgcgg tggtgcggcc cagcatcgtc gtcggcgacg agcacaccgg tgtggtgcgc
     3421 gattacaaga acatctacat cgtcctcaag ctgaccaccg agggcaaggt acgctccatc
     3481 cccggcctgt acgaggcacg tctggacctg gtgcccgtcg gctacgtggc ggacgtgatc
     3541 acgcaggtca ccgagcggtt cgacgaggcg gcgggccgca ccttccacgc ggtgggctcg
     3601 ccgctgtccc tgcgggactt ctccgacgtg atggcggagt acccctcctt ccgcgtgccg
     3661 cggttcgtcc ctccgacgag cttcgacgcg cagcggctgg aaccgcagga gcggcggtac
     3721 tacgagcgcg tcgtctcgct gtacgagagc tacttccggc gccggatgac gttcgacgac
     3781 tccaacaccg aggcactggg ggttcgccgg gcgcccgggg acgggcagga cttcctgcgc
     3841 cggctgatca accactgcct ggagtcgggc tacctgggca cgccgctggc cggcgtcacg
     3901 gacgtactga accggctgga catcacgggt ccggacacca ccggcccggc cgccaacgac
     3961 tcgaacggag ccggccgatg acacgcgaca gcgcggacgc ccgccccttc ggcgacagcg
     4021 tctcccccgc cctcagctgc gagccgcgaa aacgcgccct gttcgcctcg gacacctact
     4081 tcctggagag cctcgaacag ctcggcgccc tcaccgccga cgtggacggg tttctccacc
     4141 ggcacgccgc gctgctgctc aaacccgacg cggtggtcag caggcagata ccgacgacgg
     4201 tcgcctggct cgccgagaac ggtttccgga tcgtggcggc cgaacgcacc cggctgaccc
     4261 gtacggccgt gcggtcgctg tggtactacc agtggaacct ggccacaccg cagcggcgcc
     4321 gtctcgccga catgttcatg gactcgtgcg atgcggtggt gctcgtcgtg cgccccgtgg
     4381 cggacgaggc cgacgccgcg ccggcgtcgg tcgtcatgac ccagcgcaag ggcccgaccg
     4441 acccgctggc cagggtcccc ggccagctgc gttacgcgat cggccgctac agctacctgc
     4501 tcaacctcgt ccacaccccc gacgagcccg ccgatgtcgc gcgggagctg ggcatccact
     4561 tcgacacggc gctgcgggag cgggtctaca ccagcgcgct ggcgggcgac gaccagtcgg
     4621 cgcgggccct cgaactggcc gagcagctgc acgccgaggt accgcagcgg gacctcacct
     4681 tcgagcccgc ggcggaacgg ctcaagcagg ccgtggagga ggcggaggag tccctcctcg
     4741 gcagcccggc ccgggacgag ctgcgcgagg cccggctggc cgcccggtcc cccgagggct
     4801 accggcggct gctggaagcc gcctggcggg ccggacttcc gctcgacacc tgggacgtgg
     4861 tgatcgtcgg cacccatgtc ctgccgatga agcgcaagga cctggccccc gtactcgacg
     4921 gagtcggcgt gcaggactgg cagcttcaca tggcgcggct cgaagccgac cggtccgcgg
     4981 ccaggtaacg gaggagaagc atggacacgg tggaattcag ccgtacggtg ccccgggagc
     5041 tcgtccatcg gtcgagcgtc acggaggtcc tggtcaccgg cgcgctcgcc cgggacgacg
     5101 acaccctcga cgtcggggtg cagatacccc gctcgcacgc cttctaccgg gactcgcagg
     5161 ggtgccggca cctctacgac ccgatggtgc tggtggaagc ggcccggcag acgctcatca
     5221 tgctggggca cgatgtgttc ggcatgccgt tcggcacgaa gttcgtcctg cgggagatcg
     5281 ccctgcacga gacggacatg gccgctctca ccgtgcgcgg cgtacccacg gaggtggtgt
     5341 tgcgctgcca cgtcctgcgc cgcttcggtg atcacacggc cgtacgggga gcgcgcctgc
     5401 gctacagcgc gctgatcggc gaccacgtgg cggcgacgct ggaatgcagc atgtcctgga
     5461 tgccgcccga gcagtggcag gcgatgcgcg accagggtcg tatcgatctg ggcctggaac
     5521 cgcagctggt gcccgaggcc cccgcgccgc tgccgcgggt gcaccggacc ctggtcgacc
     5581 gtcaggaccc ctcgaacgtg gtcctctcct cgttccaggt cgcgccgggc accgtcgcgc
     5641 gagggaccgc gcagctcgtc gtcgacatgg gccaccccgt gctgttcgac cactacgtcg
     5701 accatgtccc cggcatgctg acccttgagt cgttccggca gctgggcatg gccaccgccg
     5761 tcgccaccgg ggtgctgccg tcggcgtcgg ccctgctcac cggggtgagc gcgcgcttca
     5821 ccgggatcgg tgagctggat ctgccggtca cctgcgagag cgtcctggcc cagggcgacg
     5881 ggagcagcgc cgtcctgcac tgcaccatgc ggcaggacgg gcgctccctc gccgagggcg
     5941 aagtgcgcct gacccggccc ggcgcggaga tgggaacgaa ttgaccagca cccccgtcac
     6001 cctcgacatc gacgggctgc tgtcccgtac ccccaccgtg cgccgcaggc tcgacctgtc
     6061 caggccggtc ccccggccgc tgctcgccga gtgcctccag ctcgccctgc acgcgcccaa
     6121 cggaggaaac cggcagagct ggcacttcgt ggtgatcgac gatccggcga cgaaggcggg
     6181 catcgcgcgg tactacaagt ccgcctcgga cgcctatctc gccaccacgt cctccgcggc
     6241 gatgagcgag aaggccgtgg ccggggtgcg ctacctcgcc gaccacctgc accaggtacc
     6301 cgcgctggtg ctggcgggaa tccgcgggcg gctgccccgc gacgcgccgc tcgccaagca
     6361 gtccagcttc ttcgggtccg tctatccggc gctgtggagc ttcatgctcg ccgcccgcag
     6421 ccgcgagctg ggcgccgcgc tgaccacggt gcacctcgcc cacgaacgcg aggtggcgga
     6481 gctggtcggc atccccgccg acgagttcac gcaggtcgcg ctggtggccg tcgggtacac
     6541 gacggggggc tccggcttcc gccccacccc gcggcagccg ctcgacgagg tcgtgtcctg
     6601 gaacgggtgg cagagttgaa cggcaccctg aagccctccc aggtcccggc acggctgggc
     6661 cgtttcgtac tgcggatgtt ccgcccgcag atctacgtca cctacggcgt gctgtggacc
     6721 gtgaccctgg agggcacctc cggggagatc gcgggtcccg acacgccgtg gacgccctcg
     6781 tgggcgaccg cggtgcgcgc ggcgagcgtg gtgctggcgc tgctgttcgt ccggatggtc
     6841 gacgagcaga aggatctgga ctacgaccgg cagcacaatc cggaccggcc gctggtgacg
     6901 ggcgccatca cggtcgcgga gctgcgcggc gccatggcgc tgatcgccgt cctcgtcgtc
     6961 gtactgaacc tgctggtctc ggcgtggtcg gtgctgctga tcctgctcgg tctgggatac
     7021 acgctgctcc tggtcgcgct ggaacgctgg ttcccgcgcg tcggcgaggg cctggtgacc
     7081 ggcctcctga tcagctatcc ggtgcagttg ctgctgtcgg tctacctcta cgtctcgctg
     7141 ctgaccagcg gcaagatcga ggccgatctg cgggccgtac ccctgctggc catgttcgcc
     7201 tgcgccttcc tccagttcga gttcgcgcgg aagaccgagt ggcggcagga tccgcaggcg
     7261 cggctctact cggaggtgtt cggcccccgc ggcagcgcgg cgatcagcct ggctctcgcc
     7321 gtgggcgccg tggtgctggg gctggccgtg ctggggcggg cgggtctgct gccggcggcc
     7381 gctctcctct tccccgcgct cggcgcgtgg cgcttcctcc tgcgcaagaa gtcgtccggg
     7441 ctgatgctcc cggcgatggg cttcgtgctg ttcttcaacc tgctgtgctt ctacggatcc
     7501 acagcgctcg cgatcggacg gtgagacagg tgcagccggt gatcatcgac agggtacgga
     7561 tcttcgacgg ggagtcgatg acgggtcccg gctcggtgga gttcgccgac ggcgtcatca
     7621 cgcgcgtcgt cacggacccc gcgccgcgac cgcccgcgtc ggccgaggtg atcgatggca
     7681 cgggcatgac gctgctgccg gggctgatcg acgcgcacac ccatgtgtac ggcgtcgaga
     7741 gcaacctcga actggccctg gccttcggtg tgacgaccga actggacatg ttcatgggcc
     7801 cgccggaggc caccagagcg ctgtgcgagc tggccgccga gcgtgccgac ctcgccgacc
     7861 tgcgcagtgc cgggctgctg gcgagcgcgc cgggagggca tcccggtgtc ggcatgccgg
     7921 atctgccgac cgtcgccggg cccgagcagg cggacgcctt cgtggccgcc aggcaggcgg
     7981 aaggcgcgca cttcatcaag atcatgctgg acgacggcgt aaaccacggg atgtcgctgc
     8041 cttccctcgc gccggcgacc gtgacggcgc tcgccgacgc cgcgcgccgc cggagcctgc
     8101 tcagcgtggc ccacgtgtcc gccatgtggt ccgcgcggct cgccctcgac agcggcgtgg
     8161 acatcctgac gcacctgccg ctggaagcgc ccctggacga agccttcgtc cgccgggccg
     8221 cggacggcgg gcgcgtcgtc atccccaccc tggcgatcct ggaactgtcg gcgccgcagt
     8281 cctcccccgc gaccggcgtc ggacgctcct gggcggatga cccgcggatc gccgaccacc
     8341 tgccggacca cgcccgcgcc gccatcgcgg aaggccgcga gggcctgtgt gtggagcacc
     8401 ccgtgcccga acaccgcttc ggtcacgcct tgtcgagtgt ggcccgactg caccgggcgg
     8461 gcgtgcccgt actggcgggg accgacgcca accaccggcc ggggcgcgcc tgcccggtcg
     8521 tccacggcgc cagtctgcac gccgagctgg ccctgctcgt cgaggccggc ctgactccgg
     8581 cgcaggcact cacggcggcg acctcggcac cggcggcgca cttcggcctc ggcgaccgcg
     8641 gggtgatcgc tccggggcgg cgcgcggacc tcgtcctcgt cgcgggcgac cccaccgagg
     8701 acatcaccgc cacccggtcg atcgaggcgg tctggcgggg cggcgtccgc ttcgaccgtg
     8761 aggcgcaccg caagcgcgtt gcttcatgaa cggcgggacg agcggcgata cgggcagcgg
     8821 taccggcagc aatacgagca gcgatacgaa cggcaatcac gcgaatcaca tgaaccacgt
     8881 gaacaacctg aaccatacga agcggagaac cccatgacca acgcacccga actccaggcc
     8941 ggtgtcagca gcaccgcgct ggtcgtgggc atggcccgcg cggccgagag ccggcggccg
     9001 gaccggctgt tcgaggacca tctggccgac cgtttcctgg aggcggcggg cgtcgagcag
     9061 caggcggtgt ggaacaaggg gcagggcgaa ctgttcgccg aagccatggg tgactacttc
     9121 gcgctgcgca cccgctactt cgacgactac ttccgccagg ccgccgcggc gggctgccgc
     9181 caggtggtcc tgctcgcggc cggtctggac acccgcgccc accggctgga ctggccggac
     9241 ggaacccgac tgttcgaact ggaccagccc gatgtgctcg ccttcaagga gagcgccctg
     9301 ggcgacgagg caccgcgctg ccgccgcgaa gtgatcgctg cggacctgcg cgaggagtgg
     9361 accacggtgc tggccgagcg cggcttccgc ccggaccagc cgacggcctg gctggtcgaa
     9421 ggggtgctgg tctatctgac cgagcacgac gccgacggtc tgctgagccg tatcagcgcg
     9481 ctgtcggcgc cgggcagcca cctcgccgtg gagcacgtca cccggtcgat ggtcaactcc
     9541 gaccaggccc agcaggccgc ggcggcctcg ccggaaggcg tcgtgaacct gctgtcctcg
     9601 ctgtggaaga acgagatgac gcgcccggtc ggcgactggc tcgccggcca cggctggagc
     9661 tccacccagg agtccctcac cgacctggca cagcgtcacg gccgccccgt tcccccggcc
     9721 ttcgacccgg cactgcccgg caccggccgc gtcagcctgc tcacggcggt ccgctgatcc
     9781 ctccccggcg tacggccgac cgatgaccgc cgccgccagg tgcccgaaca ggcgagagcc
     9841 atctggcacc ggcgcggaac acgtcaattc caccagcagc cgacgcccct catagtcacc
     9901 acccggcagc cgcccggggg caatccgccc cgggcggctt tcgcatgccc tgccccagcg
     9961 gcaccccgac agccggtccg ccttcgccgc gccgtatgcg tccgggccga cgtgagtgtc
    10021 actcatccat tgacccgccg cgcggcgatg gttataactt ataaccacag cactaccttc
    10081 tagctcgatc tcccgatctc agtgtggatc aggcggcaca tccatgcctc cgtacggtcc
    10141 atcgatcaat cgatccgccg aacgaggaac catgaccacc acagtcacgt cacgccaccc
    10201 ccgcgcaccc cggcacgctc cgctgtcggt gaccatcgcc tcctgggcgg tccctttcat
    10261 ggtcgtcgcc cagtacgccc tgatcgcggg cgtcccggtc gccatcgcac tggtcggcgc
    10321 cctgcgtcac gtacgcgatc gggcggtacg cctggcggcc gtcctggtcg ccgtttcgta
    10381 cgccgtcccg ctcgccgtat ggctgatacg gcccgacggg gcgcagagcc tgtccaagga
    10441 catgcacccc ggattcgccg gactcatcgt cgccgcatcc gccctgctcc tcgccaccct
    10501 ctaccgggcc cggcgtctga cacctgacac ctgacacctg acaaggaagc caccatgacc
    10561 agcacccgca tacgcgcctc cctgctcacc ctcccgctca ccgccgcggt cctcggcacc
    10621 gccgcagttc ccgccgcggc caccacccct catcacgcca agtccctcac ctgccgcggg
    10681 gcaggcgtcg atcccgacgc acgcgtccgc caccgggccg agaccgtgat ccacgcgccg
    10741 ctgcgtacca cctggaagct gcagaccgat gtggagcgct ggccgtcctg gcagtccccc
    10801 gtcacggcct cggaacgcct cgaccacggc cccctccgaa ggggttcggc cttccggtgg
    10861 acgaccccgg taccccccaa ccccacaccc gccaccagcc tggacatcac ctcgaccgta
    10921 gagcagctca aacgcaactc ctgcatccgc tggaccggcc ccgcactcgg cgaagggctg
    10981 cgca
//
