LOCUS       NZ_LWKZ01000002        10423 bp    DNA     linear   CON 28-MAR-2024
DEFINITION  Streptomyces sp. CB02923 scaffold10, whole genome shotgun sequence.
ACCESSION   NZ_LWKZ01000002
VERSION     NZ_LWKZ01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CB02923
  ORGANISM  Streptomyces sp. CB02923
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 212071)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra, Yang,D., Teng,Q., Crnovcic,I.,
            Li,X., Rudolf,J.D., Lohman,J.R., Gansemans,Y., Zhu,X., Huang,Y.,
            Zhao,L.X., Jiang,Y., Van Nieuwerburgh,F., Rader,C., Duan,Y. and
            Shen,B.
  TITLE     Strain Prioritization and Genome Mining for Enediyne Natural
            Products
  JOURNAL   mBio 7 (6), e02104-16 (2016)
   PUBMED   27999165
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212071)
  AUTHORS   Yan,X., Ge,H., Huang,T., Hindra,H. and Shen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2016) Department of Chemistry, The Scripps
            Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: CLC NGS Cell v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001905005.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 20:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,128
            CDSs (total)                      :: 8,049
            Genes (coding)                    :: 7,904
            CDSs (with protein)               :: 7,904
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 32 of 145
            Pseudo Genes (incomplete)         :: 128 of 145
            Pseudo Genes (internal stop)      :: 11 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 74486
            Orig. end                         :: 84909
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWKZ01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5423
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    881..895
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 22.26"
                     /tool="antismash"
     gene            complement(1461..2552)
                     /locus_tag="A6A06_RS06670"
                     /old_locus_tag="A6A06_33705"
     CDS             complement(1461..2552)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="A6A06_RS06670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33705"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_073760023.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 7.4e-21, bitscore:
                     68.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSESVVLHIKGRVLTGPDDVRDELWVVDGRVTYERPTGAREVTTV
                     QGWALPGLVDAHCHVGLDAHGPVDEATSEKQALTEREAGTLLLRDAGSPSDTRWIDDRE
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPADLVAYVAREARRGDGWVKLVGDWIDRETGD
                     LSACWPRGAVEAAIAEAHRLGARVTAHCFAGETLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPTLAAGGEKKFPAWASHMRSLHERRYDTVRAAYDAGVPIFAG
                     TDAGGSLAHGLIAQEVAELTKAGIPAVDALAATTWGARRWLGRPGLEEGAPADLVVYEG
                     DPRSDVRVLAAPKRVVLRGRVVG"
     misc_feature    2845..2860
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     misc_feature    2854..2871
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.23"
                     /tool="antismash"
     misc_feature    complement(2856..2871)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2866..2881)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(3013..3028)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    complement(3076..3090)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.66"
                     /tool="antismash"
     gene            3089..3604
                     /gene="ectA"
                     /locus_tag="A6A06_RS06675"
                     /old_locus_tag="A6A06_33710"
     CDS             3089..3604
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986839.1"
                     /locus_tag="A6A06_RS06675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33710"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_073760026.1"
                     /transl_table=11
                     /translation="MPEGFKLDTPRVEDGAAIWRIARDSRTLDLNSSYSYLLWCRDFAA
                     TSVVARDGEGEPAAFVTGYLRPESPETLFIWQVAVDEAHRGHGLAAALLDGLVARAAVE
                     LGVCRIETTITEDNAASNRLFTSFAERHGASLTREVLLDAGHFPEQGHHAEVLHRIGPL
                     GPPAGHRP"
     gene            3670..4938
                     /gene="ectB"
                     /locus_tag="A6A06_RS06680"
                     /old_locus_tag="A6A06_33715"
     CDS             3670..4938
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 3.3e-74.
                     Score: 241.8. Matches aSDomain:
                     nrpspksdomains_A6A06_RS06680_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="A6A06_RS06680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33715"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073760028.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.7e-79, bitscore:
                     259.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTIFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLETFQNTILRPRD
                     LPYKVMFPGPTGANAVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDSGSGLNTPAAVIVETVQGEGGINVARA
                     EWLRALSELCKRRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIIESHLR
                     AIVEEHPASIAEYRGRGMVWGLECSDKPLANKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        3757..4764
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A06_RS06680_Aminotran_3.1"
                     /evalue="3.30E-74"
                     /label="A6A06_RS06680_Aminotran_3.1"
                     /locus_tag="A6A06_RS06680"
                     /protein_end="365"
                     /protein_start="29"
                     /score="241.8"
                     /tool="antismash"
                     /translation="DRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIER
                     DGVTHGLDMSTTAKRAFLETFQNTILRPRDLPYKVMFPGPTGANAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGALAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFERL
                     LEDSGSGLNTPAAVIVETVQGEGGINVARAEWLRALSELCKRRDMLLIVDDIQMGCGRT
                     GAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     AAAALDTYWADGQMEKQTLARGEIIESHLRAIVEEHPASIAEYRGRGMVWGLECS"
     gene            5001..5423
                     /locus_tag="A6A06_RS06685"
                     /old_locus_tag="A6A06_33720"
     CDS             5001..5423
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986837.1"
                     /locus_tag="A6A06_RS06685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33720"
                     /product="ectoine synthase"
                     /protein_id="WP_073760030.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-54, bitscore:
                     176.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHTITPGTMYLLDGHEKHTMRIKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEPEPSAQHA"
     gene            5511..6410
                     /gene="thpD"
                     /locus_tag="A6A06_RS06690"
                     /old_locus_tag="A6A06_33725"
     CDS             5511..6410
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="A6A06_RS06690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33725"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073760032.1"
                     /sec_met_domain="PhyH (E-value: 2.1e-39, bitscore: 129.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVITPRQDPVVWSQPGTEGPFQPAELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVNDPAMRADARSIVEPQSQEIRSVFEVHKI
                     SEVFAKLVADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLS
                     NMRAVSVSIALTENFDTNGGLMIMPGSHRHFLGCSGETPRDNYKKSLQMQDAGTPSDEA
                     LTKMADAHSIKLFTGKAGSATWFDCNCMHGSGDNITPYPRSNVFVVFNSVENTAVDPFA
                     APVPRPDYIGARDFTPVR"
     gene            complement(6453..7712)
                     /locus_tag="A6A06_RS06695"
                     /old_locus_tag="A6A06_33730"
     CDS             complement(6453..7712)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436209.1"
                     /locus_tag="A6A06_RS06695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33730"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_079193847.1"
                     /transl_table=11
                     /translation="MAAGGRNGRSGTGGAGGSGGPEDPGSPTGPDGLGEPGGPSAAHEE
                     VSALLAAWALGAGMPGDDARVHGHLHDCAPCAAEARRLRETVRLLDEPAPGGTGRGAGR
                     KANAGMARGRLMAAARAARPGQVPLAAHAAPYAGAVACLDALLRELDRAPARWGTPVVH
                     DWDVQGTVAHLVAADEVLAERLGLAPVTEAGTGLGPVPGARPEGGFGPEGPEAGTGPGE
                     LPTGRAAGAGAPWEARWAARTNEVVAYEHTRPPAETREAWRTQAHGLLALPEAGDEQLA
                     AMATTLMGLRLPVADHYVIRAFETWVHTRDIGRALGRTVPPPLPTHLQRFLGLAVRILD
                     LALGPDARPVLLSVEGEAGGDWVLGSDAEPIAAELVLEATDFLLLVGGRQSPDEIARGQ
                     AGDAAASQRLLETATSLAWL"
     gene            complement(7802..8380)
                     /locus_tag="A6A06_RS06700"
                     /old_locus_tag="A6A06_33735"
     CDS             complement(7802..8380)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985689.1"
                     /locus_tag="A6A06_RS06700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33735"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_242440603.1"
                     /transl_table=11
                     /translation="MDGTTRYPPRDRELHRRLVYGDESALGEAYDAYGALVRAVARRVT
                     GSAAVADEVTQRVFIELWTCPYAFEPGHGSLRAWLSIRGHHGAVIRAREAEAERAAGGT
                     EPEAAPESVEEARAALHTALTELPLPEREALHLACFAGRTYRQVAVELGIAEAAATARL
                     RNALCGLADRLTEPPAGPGSPADEARDEC"
     misc_feature    complement(8393..8408)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
     gene            8536..9846
                     /locus_tag="A6A06_RS06705"
                     /old_locus_tag="A6A06_33740"
     CDS             8536..9846
                     /NRPS_PKS="Domain: Abhydrolase_1 (47-249). E-value:
                     2.8e-20. Score: 64.9. Matches aSDomain:
                     nrpspksdomains_A6A06_RS06705_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985688.1"
                     /locus_tag="A6A06_RS06705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6A06_33740"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_073760037.1"
                     /sec_met_domain="PF00561 (E-value: 3.7e-21, bitscore: 69.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVDRLPGVVLTDHFLDVPLDHAAPDGERLTVYGREAVAPGREDE
                     ALPWLLFLQGGPGCASPRPLGRDSWLVRALDHYRVLLLDQRGTGRSAPANRQTLALRGG
                     AHEQAAYLAHFRADAIVRDAEMFRRELAGPGERWSVLGQSFGGFCAMTYLSYAPEGLRE
                     VMITGGLPGLRASAEDVYRAAYPRVARKNAAHYARYPQDIERARRIAAHLREHTVRLPG
                     GGRLTTEAFQALGKMLGTGTGSYVLHYLLEDAWVTGPTGPELAESFLQAVQSHLSHTAV
                     PLYAVLHEAIYAQRSVDPKSTGWAAQRVRTEFPAFDVDTALDGGRPVHFTGEMVYPWLF
                     DTDPSLRPLKETAEALAERTDWPDLYDADRLAANDVPAAAAVYADDMYVDVAHSLETAR
                     ETRGLRTWVTNEWEHDGLRVSDGAVLDRLRRMVRGEI"
     aSDomain        8677..9282
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A6A06_RS06705_Abhydrolase_1.1"
                     /evalue="2.80E-20"
                     /label="A6A06_RS06705_Abhydrolase_1.1"
                     /locus_tag="A6A06_RS06705"
                     /protein_end="249"
                     /protein_start="47"
                     /score="64.9"
                     /tool="antismash"
                     /translation="PWLLFLQGGPGCASPRPLGRDSWLVRALDHYRVLLLDQRGTGRSA
                     PANRQTLALRGGAHEQAAYLAHFRADAIVRDAEMFRRELAGPGERWSVLGQSFGGFCAM
                     TYLSYAPEGLREVMITGGLPGLRASAEDVYRAAYPRVARKNAAHYARYPQDIERARRIA
                     AHLREHTVRLPGGGRLTTEAFQALGKMLGTGTGSYVLHY"
ORIGIN
        1 ccggtgacct gcccgcctcc gaccgggcgg cgcggcgggc cgtcaccgtc ttccccctgc
       61 tggtcatagc ggccggcgcg gccgggctgc tgtggcccgc cccgttcacg ggctgggcgc
      121 ccgcggtgcc gtggctgctc ggcgtggtga tgttctcaat ggggctgacg ctgacgccgc
      181 cggacttcac ggcggtcgcc aagcggccct gggcggtggg cctgggcctg gtcgcccact
      241 acgtgatcat gccgggactc ggctggctga tcgcgaccgt cctggacctg ccgccgcagc
      301 tcgcggccgg cgtgatcctg gtcggctgcg cgcccagcgg caccgcctcc aacgtcgtga
      361 cctacctggc ccgcggcgac gtcgccctgt ccgtctcggt ggcgacggtc tccaccgtcc
      421 tcgcgccgct gatcacaccg ccgctgacgc tgctgctcgc cgggcagttc ctggacgtgg
      481 acgccggggt catggtcacc gacatcctca agaccgtgct gctgccggtg ctcggcgggc
      541 tgctggtccg gttcgtcgcg ggccgctggg tcgaccgggc gctgcgcgca ctgccgtggc
      601 tgtccgccgt gacgatcgcg gcgatcgtgc tcgtcgtcgt gtccggcagc gccgccctca
      661 tcaaggacgc ggccgcgctg gtcatggtcg cggtcgtcct gcacaacggc ctgggcctgg
      721 ccctcggtta cgcggcgggc cggctcgccc ggctgggcaa accggccgga cgggccatgg
      781 ccttcgaggt cggcatgcag aactccggcc tcgcggcatc cctggccacc gcccacttca
      841 gcccggcagc ggcactcccc gcagccgtct tctccgtatg gcacaacatc tcgggcgcgg
      901 tggtggcggc atggatggcc cgcagcgaac gcgaacgcta gccgccacac cacacggacc
      961 cggccctccg gactccgtcc tgcggcccgc cccctcccgc cgtacagcga ccaacagccg
     1021 gtgggggcag gcgtccccca cccccgtacg tgtcagccga acacgcacgg gagggtccgg
     1081 aaccgtagga cgaagtccgt aggggccgga cccgcaccac tcccgacccg cccgcagtcc
     1141 aacggacttt cggagcccga cggcgggccg aactccgtgg ccgactggcc cccacgtgcc
     1201 gggcgacgcc cgccggagac cggacacggg aacggccctc cggactccgt cctacggccc
     1261 gccccctccc gccgtacggc gatcaacggc cggtgggggc aggcgtcccc ccacccacgc
     1321 cgcgaaagcc gaacacgtac gggagggtcc gggaccggag gacgaagtcc gcagggcccg
     1381 gacccgcgcc acggcgggtg accgccacac ccggccggcc cccggccacc gcacaccgga
     1441 aaaaccgacc cgcaagcggg tcatcccacc acccgccccc gcaacaccac ccgcttcggc
     1501 gcagccagaa cccgtacgtc cgagcgcgga tccccctcgt agacgacgag gtccgccggg
     1561 gcaccttcct ccaggcccgg ccgccccagc caccgccgcg cgccccacgt cgtcgccgcg
     1621 agcgcgtcca ccgcggggat tccggccttg gtcagttcgg cgacctcctg cgcgatcagg
     1681 ccgtgtgcga gcgatccgcc cgcatcggtg ccggcgaaga tgggcacccc ggcgtcgtac
     1741 gccgcgcgta cggtgtcgta gcggcgctcg tgcaggctcc tcatgtggct cgcccaggcg
     1801 gggaacttct tttcaccacc ggccgccagg gtggggaagg tggcgatgtt cacgagcgtg
     1861 gggacgatcg cgacgccgcg ttcggcgaac agcgggatgg tctcctcggt gaggccggtg
     1921 gcgtgttcga tgcagtcgat gcccgcttcg accagcgggg cgagggtttc cccggcgaag
     1981 cagtgggccg tcacccgggc cccgaggcgg tgcgcctcgg cgatggccgc ctcgacggcg
     2041 ccgcgcggcc agcaggccga caggtcgccg gtctcgcggt cgatccagtc gccgaccagc
     2101 ttgacccagc cgtcgccgcg ccgggcctcc cgcgcgacgt aggcgaccag gtccgcgggc
     2161 tcgatctcgt gggcgtagtt gcggatgtag cggcgggtac gggcgatgtg ccggccggcc
     2221 ctgatgatcc gcggcaggtc ctcgcggtcg tcgatccagc gggtgtcgga cggcgaaccg
     2281 gcgtccctga gcagcagcgt tcccgcctcc cgctcggtca gcgcctgctt ctcgctcgtc
     2341 gcctcgtcga ccggcccgtg ggcgtcgagc ccgacgtggc agtgggcgtc gaccaggccg
     2401 ggcagcgccc agccctgaac ggtcgtcacc tcccgcgcgc ccgtcggccg ttcgtacgtc
     2461 acccggccgt cgacgaccca gagttcgtcg cgcacgtcgt ccggcccggt caggaccctg
     2521 cccttgatgt gcagcaccac gctctcactc atggccgcac tgtatgacgc gcggtacggc
     2581 gggcggcacg gggaccggtg cacgggccac gggtcccgga cggggccacc ggcggaagcg
     2641 gaacgcaccc gtaccgcgcc cctgacgcct caccccgcac tccgcttcac agaaacaccg
     2701 tggacgcccg gccttttctt gcggtacacc ttttaccggc gaatttccaa ttctccagtg
     2761 cgcagcttcc cgtattcttc tgcccgcttt ttcgcagctc aaacgccaag atttcgctac
     2821 cgccggagta ggtaattccg agcggtcacc aggacgttac ggggttgtga ccgaccacac
     2881 aagcagtccg cttcgccccg tattttccgg gcaaaccggc acataatgcg gtcgattcgc
     2941 gcgctcgtgc gcccccgcgc ggtaacacaa gaaccccacg ccacgtaacc cctccccgcg
     3001 atgcattttc ctttttccct gggtaaattg aattcgcatg accgccgcac aagcagacca
     3061 tgcaggtgcc cgaagcgata tcagagaaat gccggaggga ttcaagctcg acacaccgcg
     3121 cgtggaggac ggagccgcga tctggcgcat cgcccgcgac tccaggaccc tggacctgaa
     3181 ctcctcctac agctacctgc tgtggtgtcg cgacttcgcc gccacctccg tcgtcgcccg
     3241 cgacggcgag ggcgagccgg ccgccttcgt caccggctac ctccgccccg agtccccgga
     3301 gacgctcttc atctggcagg tcgccgtcga cgaggcgcac cgcggccacg gactggccgc
     3361 cgccctcctg gacgggctgg tcgcccgagc cgccgtggaa ctgggcgtgt gtcgcatcga
     3421 gaccacgatc accgaggaca acgcggcgtc caaccgtctc ttcacctcgt tcgccgagcg
     3481 ccacggagcg tccctgaccc gcgaggtcct cctcgacgcc gggcacttcc cggagcaggg
     3541 gcaccacgcc gaggtgctcc accgcatcgg cccgctcggt ccccctgccg ggcaccggcc
     3601 ctgaccgacc accccgcagc ccccgcagac ttctccccgc tcacgccact catctcccag
     3661 gagcatgttg tgaccatcac ccagcccgac ctgagcgtct tcgagaccgt ggagtccgag
     3721 gtccgcagct actgccgcgg ttggcccacg atcttcgacc gcgcgcaggg cagccacatg
     3781 tacgacgagg acggccacac gtacctcgac ttcttcgccg gcgccgggtc gctcaactac
     3841 gggcacaaca accccgtact caaacgcgcc ctgatcgact acatcgagcg ggacggcgtc
     3901 acgcacggcc tggacatgtc caccacggcc aagcgcgcgt tcctggagac cttccagaac
     3961 accatcctgc gcccgcgcga cctgccgtac aaggtcatgt tcccgggccc gacgggcgcc
     4021 aacgccgtgg aggccgcgct caagctggcc cgcaaggtca agggccgcga gtcgatcgtc
     4081 tccttcacca acgccttcca cggcatgtcg ctgggcgccc tcgcggtcac cggcaacgcc
     4141 ttcaagcgcg ccggtgccgg catcccgctc gtgcacggca cgcccatgcc gttcgacaac
     4201 tacctcgacg gcacctaccc ggacttcctc tggttcgagc gcctgctgga ggacagcggc
     4261 tccggcctga acacgccggc cgccgtgatc gtcgagacgg tgcagggcga gggcggcatc
     4321 aacgtggccc gcgccgagtg gctgcgtgcc ctgtcggagc tgtgcaagcg ccgcgacatg
     4381 ctgctgatcg tcgacgacat ccagatgggc tgcggccgta ccggcgcgtt cttctccttc
     4441 gaggaggcgg gcatcgtgcc ggacatcgtc accgtctcga agtccatcag cggctacggc
     4501 ctgccgctcg cgctcaccct gttcaagccg gagctggacg tgtgggagcc gggcgagcac
     4561 aacggcacct tccgcggcaa caacccggcc ttcgtcaccg ccgcggcggc cctggacacg
     4621 tactgggccg acggccagat ggagaagcag acgctggccc gcggcgagat catcgagtcg
     4681 catctgcgcg ccatcgtcga ggagcacccc gcctccatcg ccgagtaccg cggccgcggc
     4741 atggtctggg gcctggagtg ctccgacaag ccgctcgcca acaagatcgc caagcgcgcc
     4801 ttcgagctgg gtctgctcat cgagacctcc ggcccggaga gcgaggtcgt caagctgctg
     4861 ccggcgctga cgaccacccc cgaggagctg gacgagggtc tgcgcatcct cgcccgcgcg
     4921 gtccgcgact gcgcctgagc gcaccgcgca gaccgaccgc accattcctg gtccgcacca
     4981 cagaaaggta cgagaacacc gtgatcgtcc gatccttcaa ggacatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcg ggcacgtggg agagcaagcg catcgtgctc gccaaggagc
     5101 gcgtcggctt ctcgctgcac gagaccaccc tgtacgcggg taccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtgctgtgca cggagggcga ggccgagctc accaacgacg
     5221 agaccggtga gaagcacacc atcaccccgg gcaccatgta cctgctcgac gggcacgaga
     5281 agcacaccat gcgcatcaag gaggacttcc gctgcgtgtg tgtcttcaac ccgccggtga
     5341 caggccgcga ggaccacgac gagaacggtg tgtacccgct gctcaccgag cccgagccgg
     5401 agccgtcggc gcagcacgcc tgacgcgctc ggcgcgaacc cctgccccgt ccggcagctg
     5461 aggaaggcgc gagaaggaaa gcacgtacga gaggagagga aggcaacacc atgaccaccg
     5521 cacccgagcg caccgccgac ctgtatccga cccggggagc caccgaggtg atcaccccgc
     5581 gacaggaccc ggtggtgtgg tcgcagcccg gcacggaggg acccttccag ccggccgagc
     5641 tgagcgactt cgaacgcgac ggtttcttcg ccatcgggga gctgctgacg gcggaagagg
     5701 tcgcggtgta ccgcgccgaa ctggaccggc tggtcaacga cccggcgatg cgggcggacg
     5761 cccgctccat cgtcgagccg cagtcccagg agatccggtc cgtcttcgag gtgcacaaga
     5821 tcagcgaggt gttcgccaag ctggtcgccg acccgcgcgt ggtgggtcgt gcccgtcaga
     5881 tcctcggttc ggacgtctac gtccaccagt ccaggatcaa cgtcaagccg ggcttcggtg
     5941 cctccgggtt ctactggcac tccgacttcg agacctggca cgccgaggac gggttgtcga
     6001 acatgcgggc cgtgtcggtc tcgatcgcgc tgacggagaa cttcgacacc aacggcggcc
     6061 tgatgatcat gccggggtcg caccggcact tcctcgggtg ctccggcgag acgccgcggg
     6121 acaactacaa gaagtcgctg cagatgcagg acgcgggcac gccctcggac gaggcgctga
     6181 ccaagatggc cgacgcgcac agcatcaagc tgttcaccgg caaggccggt tcggccacct
     6241 ggttcgactg caactgcatg cacggttccg gcgacaacat cacgccgtac ccgcgcagca
     6301 acgtcttcgt cgtgttcaac agcgtggaga acaccgcggt ggaccccttc gcggcgccgg
     6361 tcccgcgccc ggactacatc ggcgcgcgtg acttcacgcc cgtgaggtag cgcccacgcc
     6421 gtacggtgcc ggcggggcac cacggcacgg cgtcacagcc aggccagcga cgtggccgtc
     6481 tccagcagcc gctgcgaggc ggccgcgtcg cctgcctggc cgcgcgcgat ctcgtccggg
     6541 ctctgccgcc cgccgacgag cagcaggaag tcggtggcct ccaggaccag ttcggcggcg
     6601 atcggctcgg cgtccgagcc gagcacccag tcgccgcccg cctcgccctc caccgacagc
     6661 agcaccggcc gcgcgtccgg gcccagcgcc aggtccagga tgcgcaccgc cagccccagg
     6721 aaccgctgga ggtgcgtcgg cagcggcggc ggtacggtac ggcccagcgc ccgcccgatg
     6781 tcccgggtgt gcacccaggt ctcgaacgcg cggatcacgt agtggtcggc gaccggcagc
     6841 cgcaggccca tcagggtcgt ggccatggcg gccagctgct cgtcgcccgc ctcgggcagc
     6901 gcgagcagcc cgtgtgcctg ggtacgccac gcctcccggg tctcggcggg cggccgggtg
     6961 tgctcgtagg ccaccacctc gttcgtacgg gccgcccagc gcgcctccca gggagcaccc
     7021 gcaccggccg cccggcccgt gggcagctcg cccggcccgg taccggcctc cgggccctcc
     7081 ggtccgaagc ccccttccgg ccgtgccccg ggcaccggcc ccaggcccgt gcccgcctcc
     7141 gtcaccggcg ccagccccag ccgctcggcc agcacttcgt cggccgcgac caggtgcgcg
     7201 acggtgccct ggacgtccca gtcgtgcacg acgggggtgc cccagcgggc gggcgcgcgg
     7261 tccagctccc gcagcagggc gtccaggcag gcgaccgccc cggcgtacgg ggcggcgtgc
     7321 gcggccaggg gtacctggcc cggcctggcg gcacgggcgg cggccatcag ccggccgcgc
     7381 gccatccccg cgttcgcctt gcgtccggct ccgcggcccg tcccaccggg cgccggttcg
     7441 tccagcagcc gtacggtctc ccgcagccgc cgcgcctcgg ccgcgcaggg ggcgcagtcg
     7501 tgcaggtggc cgtggacccg cgcgtcgtcg cccggcatgc cggcgccgag cgcccatgcc
     7561 gcgagcagcg cgctgacctc ttcgtgtgcc gcggacgggc cgccgggttc gccgaggccg
     7621 tccggaccgg tcgggcttcc cgggtcctcc ggccctccgc tcccgcccgc accgcccgta
     7681 ccgctccggc cgttcctgcc tccagcagcc atcgcacgcc ccttttccct gttccgccgc
     7741 ccggccggtc cgcgcgcgca ccctgtcgct tccctagttg cccgcgggcg ccgcccggca
     7801 ttcagcactc gtcccgtgct tcgtcggccg ggctgcccgg accggcgggc ggctcggtga
     7861 gccggtcggc caggccgcac agggcgttcc gcagccgggc ggtggccgcc gcctccgcga
     7921 tgcccagctc caccgcgacc tgccggtacg tgcgtccggc gaagcacgcc aggtgcagtg
     7981 cttcgcgctc cggcaggggc agttcggtga gcgcggtgtg cagggccgcc cgcgcctcct
     8041 cgacggattc cggcgccgcc tccggttccg taccgcccgc cgcgcgctcc gcctccgcct
     8101 ccctcgcccg gatcaccgcg ccgtggtgcc cgcggatgct cagccaggcg cgcagcgagc
     8161 cgtggccggg ctcgaaggcg tacgggcacg tccacagttc gatgaagacg cgctgcgtca
     8221 cctcgtcggc gaccgccgca ctgccggtga cccgccgggc caccgccctc accagcgctc
     8281 cgtaggcgtc gtacgcctcg cccagcgcgg attcgtcgcc gtacaccagc cgccggtgca
     8341 gctcccggtc gcgcggcggg tacctggttg tcccgtccac cggcaccacc tcgtgagtgt
     8401 gtggtgacat tcctagcctt tcgccctcct tcgcggtagc cgtccgtccg gccgaattgc
     8461 gcggcgcccg gcgggtgccg cacgatcccc agggcgtccg agggacgcgc ggttcgaaaa
     8521 gaccaaggag cgtacatgag cgtcgaccgg ctgcccggtg tcgtactgac cgaccacttc
     8581 ctggacgtgc cgctggacca cgccgccccg gacggcgaac ggctgacggt gtacggacgc
     8641 gaggccgtcg cccccggccg ggaggacgag gcgctgccct ggctgctctt cctccagggc
     8701 ggccccggct gcgcgtcgcc gcgcccgctg ggccgggact cctggctggt gcgcgcactg
     8761 gaccactacc gggtgctgct gctggaccag cggggcaccg gccgctccgc gccggccaac
     8821 cggcagacgc tggcgctgcg cggcggcgcg cacgagcagg ccgcgtatct ggcgcatttc
     8881 cgcgccgacg cgatcgtgcg ggacgccgag atgttccgcc gcgagctggc cggtcccggt
     8941 gagcggtgga gcgtcctggg gcagagcttc ggcggcttct gcgccatgac ctacctctcg
     9001 tacgcgcccg agggcctgcg cgaagtcatg atcacgggtg gcctgccggg gctgcgggcg
     9061 agcgcggagg acgtctaccg ggccgcgtac ccgcgggtgg cccgcaagaa cgccgcgcac
     9121 tacgcgcgct acccgcagga catcgagcgc gcccgccgca tcgccgcgca cctgcgcgaa
     9181 cacacggtcc ggctgcccgg cggcggccgg ctgacgacgg aggcgttcca ggcgctgggc
     9241 aagatgctgg gaacgggcac cggctcgtac gtcctgcact acctgctgga ggacgcgtgg
     9301 gtgaccggcc ccacgggccc cgaactggcg gaatccttcc ttcaggccgt acagagccac
     9361 ctctcccaca ccgccgtccc gctgtacgcc gtcctccacg aagcgatcta cgcccagcgg
     9421 tcggtggacc ccaagagcac cggctgggcg gcccagcgcg tccgcaccga gttccccgcg
     9481 ttcgacgtgg acactgcgct ggacggcggg cggccggtcc acttcaccgg tgagatggtc
     9541 tacccgtggc tgttcgacac cgacccgtcg ctgcgccccc tcaaggagac cgcggaggcc
     9601 ctggccgagc gcaccgactg gcccgatctg tacgacgccg accgcctggc ggccaacgac
     9661 gtcccggccg cggccgccgt ctacgccgat gacatgtacg tcgacgtcgc ccactccctg
     9721 gagaccgcgc gggagacccg cgggctgcgg acctgggtga ccaacgagtg ggagcacgac
     9781 gggctgcggg tcagcgacgg cgcggtgctg gaccgcctgc ggcggatggt ccgcggggag
     9841 atctgaggcg ggggagatct gaggcgggga agatccgcga ccggggagat ccgcggccgg
     9901 agagatccgc gaccggggag atctgagtcg ggcagagtgg ggcgggccag tggggcgggc
     9961 tctccccgcc acctccccgc taccgcccct cccccgccac ctccagcaac cggtccacat
    10021 cgtccgacga gttgtagaga tggaaggcgg cgcggaggct gcccgcgcgc acggagaccc
    10081 gtacccgcgc cgcctccagc cggtccgccg cgtccgcgag ccccggcacg gacacgatcg
    10141 ccgagccggg tgcgggcagc ggggtgtggc cgagcgccct gacccccgcg cggtaccggt
    10201 cggcgagcgc ggtgttgtgc gcgtgcacgg tctcgacacc gatctcgttc agcagggcga
    10261 gggactgcgc ggcgccgacg tacgagtagt tggcgtgcgg ctcgtcgtaa cggcgcgcgc
    10321 cgggcgcgag ttccttgatc ggcccgtagt tggactccgc cgggtccgca ccggccaccc
    10381 acccggcgtg cagcgcttcc ggcccgtcgt ccccgcacac cag
//
