LOCUS       NZ_MPOH02000043        10984 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces phaeoluteigriseus strain DSM 41896 Scaffold_43, whole
            genome shotgun sequence.
ACCESSION   NZ_MPOH02000043
VERSION     NZ_MPOH02000043.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 23298)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Draft genome sequence of Streptomyces phaeoluteigriseus type strain
            DSM41896
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23298)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2017) Strathclyde Institute of Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
REFERENCE   3  (bases 1 to 23298)
  AUTHORS   Schniete,J.K., Salih,T., Algora Gallardo,L., Martinez Fernandez,S.
            and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2016) Strathclyde Institute for Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2017
            Assembly Method                   :: SPAdes v. 3.9; MeDuSa v. 1.6
            Genome Coverage                   :: 65.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001896135.2-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,918
            CDSs (total)                      :: 7,837
            Genes (coding)                    :: 6,622
            CDSs (with protein)               :: 6,622
            Genes (RNA)                       :: 81
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1,215
            CDSs (without protein)            :: 1,215
            Pseudo Genes (ambiguous residues) :: 0 of 1,215
            Pseudo Genes (frameshifted)       :: 907 of 1,215
            Pseudo Genes (incomplete)         :: 407 of 1,215
            Pseudo Genes (internal stop)      :: 30 of 1,215
            Pseudo Genes (multiple problems)  :: 120 of 1,215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:00:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6981
            Orig. end                         :: 17965
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MPOH02000043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(526..541)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.26"
                     /tool="antismash"
     gene            complement(973..1557)
                     /locus_tag="BM536_RS37535"
                     /old_locus_tag="BM536_037450"
     CDS             complement(973..1557)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449485.1"
                     /locus_tag="BM536_RS37535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037450"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_073496368.1"
                     /transl_table=11
                     /translation="MSEERLRVALVIGSTRATRFGPTIAKWFAAEALQNDAIELDVVDL
                     ADYPLPVAVTDAPEPADAATAAALSARLEQAEAFVVLTPEYNHSFPAPLKVAIDWNYTQ
                     WQAKPIGFVSYGGLSGGLRAVEQLRQVYAEMHAVTLRDTVSFHGAAATFGADGNPKEPA
                     GCSAAAKTMLDQLVWWGTALREARARRPYRA"
     gene            complement(1794..2258)
                     /locus_tag="BM536_RS37540"
                     /old_locus_tag="BM536_037455"
     CDS             complement(1794..2258)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /locus_tag="BM536_RS37540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037455"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_073496370.1"
                     /sec_met_domain="PF07366 (E-value: 2.8e-08, bitscore: 24.2,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLTPERVGAAWASLGTGDREKITEFWDENVRFEVPGLHAYSGW
                     YEGLDAFLEFFGNMFRLSGGTMHGENVTVLVNPEAGYSTDVNRITAVRAGAAADSTSPY
                     DTFAVEALHLLRWSNGRIVEGRWVVMGAGAETTALWWSPRDANGARRQVV"
     gene            complement(2397..3197)
                     /locus_tag="BM536_RS37545"
                     /old_locus_tag="BM536_037460"
     CDS             complement(2397..3197)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640230.1"
                     /locus_tag="BM536_RS37545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037460"
                     /product="response regulator transcription factor"
                     /protein_id="WP_245873991.1"
                     /transl_table=11
                     /translation="MSQPGMVRHRTDSTHHRHRTSGTQRRVSREVPGFRSGVEVAGEAW
                     RVLVVESDVDAQRGITHSLKRQGCEVVGVDSGSKALEMYEHADLVLLDLELSDLHGLDV
                     CAAIRAAGDTPIIAFTCRRSEWDQVLVLEAGADDCLLKPYDLRELTARIGAVMRRSLRP
                     ARAVDVMEWGPLRIDMKLREVTLRGARVDCTRKEFDLLCLLASQPGEVVSRRQLMSQVW
                     GEHWSPPSRTIDTHVAGLRRKLGPDEWITTIRGVGFCFEPADFH"
     gene            3517..4083
                     /locus_tag="BM536_RS37550"
                     /old_locus_tag="BM536_037465"
     CDS             3517..4083
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018521705.1"
                     /locus_tag="BM536_RS37550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037465"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_073496374.1"
                     /transl_table=11
                     /translation="MRDKAARTRSELIRSAATVFAEHGYSRAKLSSISADAGVSRGALY
                     FHFPSKEALADAVEAAAAERFAGVTGHEGDNGARDLSRAVRAFADLYRQNVIFRAGVLL
                     SCEKDRKSTVNLIQQWEEYVSTAVAAAERGYPFARSGVAGHASSAIVAMTVGLAFLWHE
                     NEQWLAESTVTGVWNLLPPLVGRMG"
     gene            complement(4165..4833)
                     /locus_tag="BM536_RS44120"
                     /old_locus_tag="BM536_037470"
     CDS             complement(4165..4833)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523578.1"
                     /locus_tag="BM536_RS44120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037470"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073496376.1"
                     /transl_table=11
                     /translation="MATQERGLATRRMILEAAGAVFSERGYSAATVSDVLKRLGLTRGA
                     VYFHFASKEELARTVLASQADIPTVVRDIKLQELIDLGLVFACQLTRDPVLQGSVRLSL
                     ELDSVGLDRKLPFRSWVDRNQEILEQARAQGELRPHVDLKETAELLCSCFTGVQLFSQV
                     MTGWADLETRIRALLKHVLPNIAEPSVLVQLDLSADRGLKVGAELSALAEAREAEENDR
                     "
     gene            5001..5984
                     /locus_tag="BM536_RS37560"
                     /old_locus_tag="BM536_037475"
     CDS             5001..5984
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="BM536_RS37560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037475"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_079275117.1"
                     /sec_met_domain="AfsA (E-value: 5.9e-21, bitscore: 65.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQAIAVSEISAPISRRGETDISADVPLYPVVKELVHRSNPSDVLP
                     VGWRPLAEDRYLISVAWPQGHPFYARVNGDHAANLIGETIRQCGLLLAHAAYDVPLGHH
                     FVMWDMAYTREPARPASAVGTHRVEVEVVCSDLRLRGRRLGAMNCDMTLRADGRTVARG
                     GGRFDIVSPAVYRRLRGAQIEAATQPVQPDPVPPAAVGRTRVMDVLLAPAPEPAPSADA
                     PFGRWLLRADFGHPTLFDHRNDHFPGMLLVEAAFQAANATVAPALHHHTSAKVAFLGYV
                     EYGHPCWIETRLRPTGMPQLTAIEVTGRQNGRPVFSALLEGASAQD"
     gene            6492..7490
                     /locus_tag="BM536_RS37565"
                     /old_locus_tag="BM536_037480"
     CDS             6492..7490
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /locus_tag="BM536_RS37565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037480"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_079275119.1"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.5e-07,
                     bitscore: 20.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5e-11, bitscore:
                     32.7, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSREHRWDGGTPTVLVTGATGFIGHRAVHRLLAHEGRPRLRLLV
                     HDRDVPECCAAATAVVRGSLTDPASLRGVCRGVGTVLHLASLITQDPATAHAVNTAGTE
                     ALLAEAHRSGVRRFVRLGTAAVYGPGPHRGEPAPSGRPLSVTSASRLAGDRSVLAAGGT
                     VLRPYLVYGAGDVWFVPALVQMITRLPRWIEGGRARLSLISVDDLARVTAALVTRPAPL
                     PASTVLHAAHPRPVVVRDLVTALCRQLGLPLPRGDVSRADLEAHLGGPADADLVRRFAQ
                     FGTDHWFDSSELWSLAGLEPGPGFAEQFAACAPWYREHLRDPLPATAPAAG"
     gene            7560..8834
                     /locus_tag="BM536_RS37570"
                     /old_locus_tag="BM536_037485"
     CDS             7560..8834
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569592.1"
                     /locus_tag="BM536_RS37570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037485"
                     /product="cytochrome P450"
                     /protein_id="WP_073496379.1"
                     /sec_met_domain="p450 (E-value: 1.4e-19, bitscore: 60.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQPTRSTQPTDATQQPATCPFAIDPGGRDIQGEISRIRARGPVTR
                     VELPGGVPAWSVTSAELIKRLLLDPRVSKDAYRHWPAWIDGEVTADWPLSIWVSVQNMV
                     TAYGTEHTRLRRPVAGAFGVRRVNAMRPRVEKIVDELLDKLGELAQAAPDSVVDLREHY
                     AHPLPSSVVSELFGVPESARGELLRVIKGFFATTSSTQEAQSNVVRLYATMNDLVALKK
                     REPGDDLTTDLIGARDDDGAVMSDKELADNLILLLTAGYETTVNLLDNAITRLLAHPEQ
                     LELVRSGRASWEDVIDESLRLDAPGAHSVLRYAVEDLEIGGVTIARGEAITISYAGAGR
                     DPALHGEDADRFDITRATRGEHLSFGHGVHYCLGAQLARMEASIALSALFARFPGLRPA
                     VPAEELRPLESFISNGHQEVLALLG"
     gene            complement(8910..9692)
                     /locus_tag="BM536_RS37575"
                     /old_locus_tag="BM536_037490"
     CDS             complement(8910..9692)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989039.1"
                     /locus_tag="BM536_RS37575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037490"
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="WP_073496386.1"
                     /transl_table=11
                     /translation="MALAALLVAALSGCSGDGRTADPAPTARPRWQPGPGLAWQWQLDG
                     KVDPSADVPVYDIDGFENSAADVARLHRDGRKVICYVNVGAWEDFRPDKDAFPRSVLGE
                     PNGWAGERWLDIRRLSVLRPLMERRFDMCRDKGFDAVEPDLMEGYGNKTGFPLTARDQL
                     RYNRMIAGLAHERGLSVGLKNDLPQIPELVGDFDFAVNEECAQYDECDRLTPFVAAGKA
                     VFHVEYTEPAGEFCPQSRRLRLSSMLKKPELGVWRRPC"
     gene            complement(9713..10375)
                     /locus_tag="BM536_RS37580"
                     /old_locus_tag="BM536_037495"
     CDS             complement(9713..10375)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740221.1"
                     /locus_tag="BM536_RS37580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_037495"
                     /product="spherulation-specific family 4 protein"
                     /protein_id="WP_094104056.1"
                     /transl_table=11
                     /translation="MSLLIPLYVHPAEDPGAWHRLITSAAGTYGVVLNPANGPGEGPDP
                     AFAAAAGALRAAGARLLGYVDTDYGTRDGAEIADEVRRHQEWYAADGCFLDRVTATPEA
                     LSACRRLVRAVRRLGAGTVVLNPGVHPAAGYTRLADLTVTFEGHWSTYVSAFSRPAWTV
                     REAPERLCHLVYGVPEALVPLAVRTAHERGAAVCGPVTGELPNPWAELSPALTGVRP"
ORIGIN
        1 ccggtcgaca ctcagggcgg cccgggtcag cagcagtccg gccgcgtcga tctcgccggc
       61 cgcccgtgcg tgggcgatcc tcagcgcctc gctggggtcg gtgccgcgtg ccgccttgtc
      121 ggcgagtgcg cgtgccgaca gctccagcgc gccccgggcg gcgccgagaa ccggtgcggc
      181 gaacgtcagg ccgttgacgg cgaacagcgg ctgggagtgg cgcggttcgt ccgagaccgc
      241 gccccggccc gcgaacatgt cgtccatgag gaaggagcgg tgcccgggga cgaacacctc
      301 ctcgacgacg agcgtgtggc tgcccgtcgc ccgcatcccc accgtgtccc aggtctcctc
      361 gatccggtag gcgtcgcggg gtatcgcgaa gaaccgtgcc tggcgctgct cgtccgcccc
      421 cgccaccatg gccatcacca gggcccagtc ggagaactcg acaccgctgg cgtacgtcca
      481 ggtcccggag agccgccagc cgccgtcggc cgccagggcg gtcgcggtgg tggagaccag
      541 ggagctgacc agccggctgt ccggcccgtc cgcccatatc tccgcctgtc cctcggcggg
      601 cagatacgcg gcgaaacggg cggtgtaggc gagcagcgag ccgatccagg cggcggaggc
      661 gcacccctcg ccgaccgcgg cgaccgcctc ggtgagggtg acgaagtcgg tcggttcgcc
      721 gccgtgccgg gccgggacca gcgccctgct gaagccggcc tcgaccagtg ccgccaccac
      781 gtcgggggag agccggcgtt cgcggtcggc ccgggccgcg tggcggcccg ccggcacacc
      841 tgcggcccgg gcggcggaca cgaaggtgtc cgaagtggta cggggagcca ggagcatccc
      901 ggtcatggct gtctcatttc atcggcttct cggctgaggt gacgcgggcc cgggcgcggc
      961 ggcggggccg gctcaggcgc ggtaggggcg cctggcgcgc gcctcacgga gtgcggtgcc
     1021 ccaccacacg agctggtcga gcatggtctt ggccgcggcg gagcagcccg ccggctcctt
     1081 ggggttcccg tccgcgccga aggtggccgc ggcgccgtgg aagctcaccg tgtcgcgcag
     1141 ggtgacggcg tgcatctcgg cgtagacctg gcgcagttgc tcgacggcgc gcagaccgcc
     1201 cgagaggccg ccgtaggaga cgaagccgat cggcttggcc tgccactggg tgtagttcca
     1261 gtcgatcgcg accttgaggg gtgcggggaa gctgtggttg tactcgggcg tcaggacgac
     1321 gaacgcctcg gcctgctcca gccgggcgct gagggccgcg gccgtcgccg cgtcagcggg
     1381 ctcgggggcg tcggtcaccg ccaccggcag gggatagtcg gccaggtcga cgacgtccag
     1441 ctcgatggcg tcgttctgca gggcctctgc ggcgaaccac ttcgcgatgg tggggccgaa
     1501 ccgggtggcg cgggtgctcc cgatcaccag ggctactcgc agacgttcct cggacatggg
     1561 gatcctcacg gtcgaaggtg tgctgtgcgg ggacgtcacc cgggagtccg ggaacggcgg
     1621 atcgtcacgc cccccatggg ctgggctggg ctgcgttggg ctgtggagtg cggtgcggtg
     1681 cggcggggcc gtccccacgt caccgccgca ccgcaccggc acccgctgcc ggtactgctg
     1741 tggcctccgc taccgccacg gtcgtaccgg ggtccggtgg agccggccgg aggtcagacg
     1801 acctggcggc gggcgccgtt cgcgtcacgc ggcgaccacc acagcgcggt ggtctcggct
     1861 ccggccccca tgacgaccca acgcccctcg acgatccggc cgttgctcca gcgcagcagg
     1921 tgcagcgcct cgacggcgaa ggtgtcgtag ggggaggtgc tgtccgccgc ggccccggcg
     1981 cgtacggcgg tgatgcggtt gacgtcggtc gagtagccgg cctcggggtt caccaagacc
     2041 gtcacgttct cgccgtgcat cgtgccgccg gagagccgga acatgttccc gaagaactcc
     2101 aggaacgcgt cgaggccttc gtaccagccc gagtaggcgt gcagtccggg cacctcgaat
     2161 cgcacgttct cgtcccagaa ctccgttatt ttctcccggt ctccggtgcc gagggaggcc
     2221 caggcggcgc cgacgcgctc gggagtgaga tcagacatgg gtggtgattc ccttcgaaga
     2281 atgggtgcgg gcggctgtcg ggccacccgt ggcgccggcg gaggatccgc tgtggaccgc
     2341 tccgccgacg gctgagcgcg gggccgcgac gacgacccgg ccggtgcgaa gccgtgtcag
     2401 tggaagtcgg cgggttcgaa gcagaacccg acaccgcgga tcgtcgttat ccactcgtcc
     2461 ggtccgagct ttctgcgcag gccggccaca tgggtgtcga tcgtgcggct gggcggtgac
     2521 cagtgctccc cccagacctg gctcatgagc tggcgccgcg acacgacctc ccccggctgt
     2581 gaggccagca gacacagcaa atcgaattcc ttgcgggtgc aatcgacgcg cgcaccgcgc
     2641 agcgtgacct cacgcagctt catgtcgatg cgcagcggtc cccactccat gacgtccacg
     2701 gcgcgggccg ggcgcaggct gcggcgcatc accgctccga tccgcgctgt caactcccgc
     2761 aggtcgtagg gcttcagcag gcagtcgtcc gcccccgcct cgaggacgag cacctggtcc
     2821 cactcgctgc gccggcaggt gaacgcgatg atgggcgtgt ccccggccgc ccggatcgcg
     2881 gcgcatacgt cgaggccgtg caggtccgac aactccaggt ccagcaggac cagatccgcg
     2941 tgttcgtaca tttccagggc cttgctgccg ctgtcgacac cgacgacttc gcagccctgc
     3001 cgcttgagac tgtgagtgat cccccgttgg gcgtccacgt cgctctcgac gacgagtaca
     3061 cgccacgcct cgccagccac ctctaccccc gacctgaaac ccggcacttc ccgtgacacg
     3121 cgacgctgcg tgccggaggt gcggtgacgg tgatgtgtgc tgtccgttct gtgccgaacc
     3181 atgcccggct ggctcatccg atgtcccccg ttcctgcgtg gacacccacg ccgaaaaccg
     3241 atcgagcact cggagaatac aaaccgacac gcatgcatgt caacgcgcgt caggtggctt
     3301 ggatcacacg ttgcgcgtct caattgctgc cgctagacag tgcaattggg gagatagcgg
     3361 acagagactg gctgtcagca accggtccga cggccgataa agtccccttg cgaacggggt
     3421 aaagatacaa gcgtgaatgt tatattcgcg tacttgcggc gcgggtccct gaccccgtgt
     3481 caccgcgagc cacctgcacc gatgtgagga gtcggcatga gagacaaggc ggcccggacc
     3541 cgctccgaat tgatacgttc ggccgcgacg gtcttcgccg aacacggcta ctccagagcg
     3601 aagttgtcct ccatctccgc ggacgcggga gtgagcagag gagccctcta tttccacttc
     3661 cccagcaagg aggccctggc ggacgcggtg gaggcggcgg cggccgagag gttcgcgggg
     3721 gtgaccggcc acgagggcga caacggcgca cgggacctgt cgcgagcggt gcgcgcgttc
     3781 gccgacctgt accggcagaa tgtcatcttc cgcgccggcg tgctcctgag ctgcgagaag
     3841 gaccgcaagt ccacggtgaa cctgatccag cagtgggagg agtacgtcag taccgccgtc
     3901 gcggcggcgg agcgcgggta tcccttcgcc cgttccggcg tggcggggca cgcctccagc
     3961 gcgatcgtcg ccatgaccgt ggggctggcg ttcctgtggc acgagaacga gcagtggctc
     4021 gccgagtcaa ccgtcacagg cgtatggaat ctgctgccgc ccctggtcgg ccgcatgggc
     4081 tgagaccgcc gcggccgggg tccgcccgca ccgggcggac cccgcccgtc ggtgcttgtt
     4141 cgacaaaccg aacatgccgg aatgtcagcg gtcgttctcc tcggcctccc gggcctccgc
     4201 cagggcgctc agctccgccc cgaccttgag tccccggtcc gcgctgaggt cgagctgcac
     4261 caggaccgag ggttccgcga tgttgggcag cacatgcttc agcagtgccc ggatccgcgt
     4321 ctccaggtcc gcccagccgg tcatgacctg cgagaacagc tgaaccccgg tgaagcagga
     4381 gcacagcagc tcggcggtct ccttcagatc gacgtgaggt cgcagctcgc cctgcgcacg
     4441 cgcctgctcc aggatctcct ggttgcggtc gacccagctc ctgaagggga gcttccggtc
     4501 gagcccgacc gagtccagct ccagtgacag ccggacgctg ccctggagga cggggtcccg
     4561 ggtcagctgg cacgcgaaca ccaggccgag gtcgatcagt tcctggagct tgatgtcgcg
     4621 cacgacggtg gggatgtccg cctggctggc cagcaccgtc cgcgccagct cctccttgga
     4681 ggcgaagtgg aagtacaccg cgccccgggt gagccccagc cgcttcagca catcggagac
     4741 cgtcgcggcg ctgtagccgc gttcggagaa gacggcccct gcagcctcca ggatcatccg
     4801 gcgggtcgcg agacctcgtt cctgcgtcgc catgggttct acctccgggg gctgcgctgt
     4861 ctgtgctggt ggagccgacg taacgagtcg ttaatctcac tatgtgagac aacccattga
     4921 gctacatacc gactcacacg tatgttacgt cccgctcggg ctcacctcga gctcagcgtc
     4981 gcattcctgg ggggaaggca atgcaagcca tagctgtaag tgagatcagt gcgcccattt
     5041 ccagacgtgg ggaaacggac atttcggccg atgtgccgtt gtatccggtc gtcaaggagc
     5101 tggtgcaccg ctcgaacccg tcggatgtgc tgccggtcgg ctggcggcct ctggccgagg
     5161 accgctatct gatcagtgtc gcatggcctc aaggccaccc tttctacgca cgggtgaacg
     5221 gcgaccacgc ggccaacctg atcggcgaga ccatccggca gtgcggtctg ctgctggcgc
     5281 acgccgccta cgacgtgccg ctgggacacc acttcgtcat gtgggacatg gcgtacaccc
     5341 gggaaccggc ccgtccggcc tcggccgtcg ggacccaccg cgtcgaggtg gaggtcgtct
     5401 gttcggacct gcgtctgcgc ggtcgtcggc tgggggccat gaactgcgac atgacgctgc
     5461 gcgcggacgg caggacggtg gcgcgggggg gcggtcgctt cgacatcgtc agcccggccg
     5521 tctaccgcag gctgcgcggg gcgcagatcg aggccgccac acagccggtc cagcccgatc
     5581 ccgtgccgcc ggccgctgtc ggccgcaccc gcgtgatgga cgtcctgctc gccccggctc
     5641 ccgagccggc cccgtcggcc gacgcgccgt tcgggcgctg gctgctgcgc gcggacttcg
     5701 ggcacccgac gctgttcgac caccgcaacg accacttccc ggggatgctc ctcgtcgaag
     5761 cggcgttcca ggccgcgaac gcgaccgtcg caccggccct gcaccaccac acctcggcca
     5821 aggtggcctt cctcggctat gtcgagtacg gccacccgtg ctggatcgag acccgcctca
     5881 ggccgaccgg catgccgcag ctcaccgcga tcgaggtcac cggtcgccag aacggccgcc
     5941 cggtgttctc cgcgctcctc gaaggcgcgt cggcgcaaga ctgacagtcc cttcgtccac
     6001 ccgcccgtcg tcgagcgggg tccgcgtcac cgccgaccgg cccgcttgtc ccgcggttcg
     6061 tccccaccgc tgccggtgac cggcgttcca ggtcgccgtc cgccgtgtcc ggtacgccgc
     6121 cgacccgcac cgtccggtcc tcgtcaccgt catcgacgcc tggtggcgcc cctccgtgtg
     6181 cccggtgcac agggcacacg cacgggcgcc gccgggcagc tcacgcacag tcctgcccgg
     6241 acgagcgccg ggccacgggc cccgcggggc gacgacccgc ttcgacggct ccgctccagg
     6301 aaaggaaccc actcctgtga cgcacgactt cacggccgga aagtccggcc ggtcatctcc
     6361 gcgcatccgc aattcctctc cgcaccggcc gagttccacc gttgacagcg ctgtcatcgt
     6421 aacgcgcgtc acggcagccg tccccgcccg tgcgcacggc tggcttgtct ctttcgcaaa
     6481 gaggcgtgat cgtgagcagt cgtgaacacc ggtgggacgg cggtacgccg accgtcctcg
     6541 tcaccggcgc gaccgggttc atcggccacc gggccgtcca ccggctgctc gcccacgagg
     6601 ggcggccgcg actgcggttg ctcgtccacg acagggacgt accggagtgc tgcgccgccg
     6661 cgacggccgt cgtccgggga agcctcaccg accccgcgtc cctgcgcggg gtgtgccgcg
     6721 gcgtcggcac cgtcctgcac ctcgcctcgt tgatcacgca ggatccggcg accgcccacg
     6781 cggttaacac cgccggtacg gaagccctgt tggccgaggc gcaccggtcc ggtgtccggc
     6841 gcttcgtacg gctcggcacc gccgccgtgt acgggcccgg cccccaccgg ggcgagcccg
     6901 cgccgtccgg ccgaccgctg tcggtgacca gcgcctcgcg actcgcgggc gacaggagcg
     6961 tcctcgccgc cggcggcacc gtcctgcgcc cgtacctggt ctacggggcg ggggacgtgt
     7021 ggttcgtgcc cgccctcgtc cagatgatca cacggctgcc gcggtggatc gagggcgggc
     7081 gggccaggct gtcgctgatc tccgtcgacg acctggcccg ggtcaccgcg gccctggtga
     7141 cccggccggc tccgctgccc gcgtccaccg tcctgcacgc ggcccatccc cgcccggtcg
     7201 tcgtacgcga ccttgtcacg gcgctctgcc ggcagctggg tctgcccctg ccgcgggggg
     7261 acgtctcacg ggccgacctg gaagcgcacc tcggcggtcc ggccgacgcg gatctggtgc
     7321 gacggttcgc ccagttcggg accgaccact ggttcgacag ctccgagctg tggtccctgg
     7381 ccgggctgga acccggcccc gggttcgccg agcaattcgc cgcctgcgct ccctggtacc
     7441 gcgagcacct gagggacccg ctgcccgcca ccgcgccagc ggccggctga accggcctcc
     7501 ctgtcacgcc actcactcca ccgcacacca cctcactcca cccgaaagcg gaatcgacga
     7561 tgcagcccac ccggtccacc cagcccacgg acgccaccca gcagcccgcg acgtgtccct
     7621 tcgccatcga tcccggcggc cgcgacatcc agggcgagat cagccgtatc cgggcacgcg
     7681 gccccgtgac ccgggtggaa ctcccgggcg gcgtaccggc ctggtcggtc accagcgcgg
     7741 aactgatcaa gcggctcctg ctggacccgc gcgtctcgaa ggacgcctac cggcactggc
     7801 cggcctggat cgacggcgag gtcaccgccg actggcccct gtccatctgg gtctcggtac
     7861 agaacatggt caccgcgtac ggcaccgagc acacccggct gcgccgcccg gtcgccggcg
     7921 cgttcggcgt ccgccgggtc aacgcgatgc gcccccgcgt cgagaagatc gtcgacgaac
     7981 tgctagacaa actgggagag ttggcgcagg ccgcgcccga ctcggtcgtg gacctcagag
     8041 agcactacgc ccacccgctg cccagcagcg tcgtctccga actgttcgga gtccccgagt
     8101 cggcacgcgg tgagctgctc cgcgtgatca agggcttctt cgcgacgacg tcgtccacac
     8161 aggaggcaca gagcaatgtc gtccgcctct acgccaccat gaacgacctc gtcgccctga
     8221 agaagcgtga gcccggcgac gacctgacca cggacctgat cggcgcccgc gacgacgacg
     8281 gcgccgtgat gagcgacaag gaactggcgg acaacctcat cctgcttctc accgccggat
     8341 acgagacgac ggtgaacctc ctcgacaacg cgatcacccg cctcctggcc catcccgagc
     8401 agctcgaact cgtccgctcc gggcgggcgt cctgggagga cgtcatcgac gagtcgctgc
     8461 gcctggacgc gcccggcgcc cactccgtgc tgcgctacgc ggtggaggac ctggagatcg
     8521 gcggggtgac gatcgcgcgc ggcgaggcga tcacgatctc gtacgccggc gcgggccggg
     8581 accccgccct gcacggtgag gacgccgacc ggttcgacat cacccgcgcc acccggggcg
     8641 aacacctctc cttcgggcac ggagtgcact actgcctggg agcccagctg gcgcggatgg
     8701 aggcgtccat cgccctgtcc gcgctcttcg cccgcttccc gggcctgcgg cccgccgtcc
     8761 cggcggagga gctgcggccc ctggagtcct tcatatccaa cggccaccag gaggtcttgg
     8821 ccctgctggg ctgagtccgc caccgacccg gtgaccgccc tgcccggacc cggaggcatt
     8881 ccgggcatcg gcgcgtcccg cccgaggtct cagcagggcc tgcgccacac ccccagttcc
     8941 ggcttcttca gcatcgagga caggcgcagc ctgcgggact gggggcagaa ctccccggcg
     9001 ggttccgtgt actccacgtg gaagaccgcc ttgcccgccg cgacgaacgg ggtgaggcgg
     9061 tcgcactcgt cgtactgggc gcactcctcg ttgaccgcga agtcgaagtc gccgaccagc
     9121 tccgggatct ggggaaggtc gttcttcagt cccaccgaca ggccgcgctc atgggcgagt
     9181 ccggcgatca tccggttgta gcggagctgg tcgcgggcgg tgagcgggaa accggtcttg
     9241 ttgccgtatc cctccatcag gtcgggttcc accgcgtcga agcccttgtc gcggcacatg
     9301 tcgaagcgcc tttccatcag cggtcgcagg acggacaggc gtcggatgtc cagccaccgc
     9361 tcgcccgccc agccgttggg ctcgccgagc accgagcggg ggaaggcgtc cttgtcgggc
     9421 cggaagtcct cccacgcgcc gacgttgacg taacagatga ccttgcggcc gtcccggtgc
     9481 agccgggcca cgtcggccgc ggagttctcg aagccgtcga tgtcgtagac gggcacgtcg
     9541 gccgacgggt cgaccttccc gtcgagctgc cactgccagg ccaggccggg accaggctgc
     9601 caccgaggtc tcgccgtcgg ggccgggtcg gcggtccggc cgtccccgga acagccgctc
     9661 aacgccgcga ccagcagggc ggccagtgcc atgaaacccg ccgcggcccg cctcacggcc
     9721 ggactccggt cagtgccggg ctcaactcgg cccaggggtt cggcagttca ccggtgaccg
     9781 gaccgcagac cgccgcgccg cgctcgtgcg cggtgcgcac ggcgagcggc accagcgcct
     9841 ccggcacccc gtagaccagg tggcacagcc gctcgggcgc ctcccgaacc gtccaggccg
     9901 gtctgctgaa cgccgagacg tacgtcgacc agtgcccctc gaaggtgacg gtcaggtcgg
     9961 ccagccgcgt gtacccggcc gccggatgga ccccggggtt gagcacgacc gtccccgcgc
    10021 ccagccgccg caccgcccgg accagccggc ggcaggcgga cagagcctcg ggggtcgcgg
    10081 tcacccggtc caggaaacag ccgtcggccg cgtaccactc ctggtgccgg cgtacctcgt
    10141 cggcgatctc cgcgccgtcc ctcgtcccgt aatcggtgtc gacgtacccc agcagccgcg
    10201 cccccgccgc ccgcagcgcg ccggccgccg cggcgaacgc cggatcgggg ccctcgcccg
    10261 ggccgttcgc cgggttgagg acgaccccgt aggtgccggc ggccgaggtg atcagccggt
    10321 gccaggcccc cgggtcctcg gccggatgga cgtacagcgg gatcagcagg ctcacgacgt
    10381 accctcgcct ccccacagcg cggcgagggc gtcgtccagg gagtggaccg ggcgccagcc
    10441 gagggcacgc gccgcggcgc cgatgtccga gcactgccag gagacctccg cggaacgggc
    10501 cgagcccgcc gtacccgccg cgttctcctc gatccgcccc gcgaaggccg cgagccgcac
    10561 cagccgccgc accagctcgc gcaccgggac cgcccggcca ccaccgatgt tgaggaccgg
    10621 cggcagcggc ccgggcgccg tgaccgccag cgccacggca cccgccacat cgcgtacgtc
    10681 cacgaagtcg cggaacgccg acaggtcgcc cagccgcagc accgcctccg ggtccgtgcc
    10741 cgccgcccgc agcagcccgg cgatccggcc cggcagcccc gaggcgggcg cccccggccc
    10801 caccgggttg cccacccgca gcaccaccgc gtccagcccg gaggcggtca ccgcggccgt
    10861 ccccgcgagc ttggtggcgc cgtacgggcc ggccgggctg gtggccgccg actccgtcac
    10921 ccgggagtcc acggcgccgg ggccgtactc ggcggccgag ccgaggtgga ccagccgcgc
    10981 cgcc
//
