LOCUS       NZ_MPOH02000017        10216 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces phaeoluteigriseus strain DSM 41896 Scaffold_17, whole
            genome shotgun sequence.
ACCESSION   NZ_MPOH02000017
VERSION     NZ_MPOH02000017.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 367483)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Draft genome sequence of Streptomyces phaeoluteigriseus type strain
            DSM41896
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 367483)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2017) Strathclyde Institute of Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
REFERENCE   3  (bases 1 to 367483)
  AUTHORS   Schniete,J.K., Salih,T., Algora Gallardo,L., Martinez Fernandez,S.
            and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2016) Strathclyde Institute for Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2017
            Assembly Method                   :: SPAdes v. 3.9; MeDuSa v. 1.6
            Genome Coverage                   :: 65.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001896135.2-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,918
            CDSs (total)                      :: 7,837
            Genes (coding)                    :: 6,622
            CDSs (with protein)               :: 6,622
            Genes (RNA)                       :: 81
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1,215
            CDSs (without protein)            :: 1,215
            Pseudo Genes (ambiguous residues) :: 0 of 1,215
            Pseudo Genes (frameshifted)       :: 907 of 1,215
            Pseudo Genes (incomplete)         :: 407 of 1,215
            Pseudo Genes (internal stop)      :: 30 of 1,215
            Pseudo Genes (multiple problems)  :: 120 of 1,215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:00:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 341103
            Orig. end                         :: 351319
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MPOH02000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            258..2405
                     /locus_tag="BM536_RS29040"
                     /old_locus_tag="BM536_028955"
     CDS             258..2405
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128986.1"
                     /locus_tag="BM536_RS29040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_028955"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_245873861.1"
                     /transl_table=11
                     /translation="MVAVSDEQLSGRAPGDPAGRSMLAPALTAMMDAVGAHSGAVYLLP
                     DDEPVLEMAVMAGLPRSFAAPWQRVGVGAPIPIARAVRERRLVWVGGEEEMARSYPRIA
                     VVLPYPFALAALPLATGSKVYGAVFVTWPGSHPPELSDRERHELQAACERLALRLERAA
                     AENRPVRGESDPLDTRPAAADTLGTVEAARLVARLPYGLCSLDLSGRVGFANAAAADLL
                     GVPVGELLGTHLWASVPWLADPMYEERYRAALISQHVTSFVALRPPSAWLSFRLYPSTT
                     GISVRVVRARAVSVTSPAGTEGDDAPPRLVSMSQALSLAGALTETVSVQDVVRLVAEEI
                     APAVGSGALALLGSRAGRLHVLGHHGYPDPHLVERFDGMPLASPTPGTHAVTHGVPAFF
                     DSRRQLEHLYPARHATPDGFAAWAYLPLIASGRPVGVCVLAYAEPHSFSTHERAVLTSL
                     AGLIAQALDRALLYDAKHRLAHGLQAALLPHSLPSLPGIEASARYLPATRGMEIGGDFY
                     DLVPSRPLPAAVIGDVQGHNVTAAGLMGQLRTAVRAYTTVGQGPAEVMRSTNRLMIDLG
                     TELFASCLYLRLDPAHGRAVMARAGHPPPLLRRPDGRVRVLDLAGGPLLGIDAEATYPT
                     TEITLSPGCVLALYTDGLIESPGVDIEDAVADLGRRLGEAGDLPLEALADELVGHYAAA
                     EERIDDVALLLLRASSARDRG"
     gene            complement(2551..3945)
                     /locus_tag="BM536_RS29045"
                     /old_locus_tag="BM536_028960"
     CDS             complement(2551..3945)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134775.1"
                     /locus_tag="BM536_RS29045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_028960"
                     /product="S1 family peptidase"
                     /protein_id="WP_094103692.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAAIAVGGVAALGAAALLLPHANASQDAGDTAAAAP
                     RTMKAADASDLASQLQNLLGDAFAGSYFDTGKNQLVVNVVDVSGDQNNVIVQAKKAGAK
                     VREVENSTAELKAAAQTLKADATIPGTSWAVDPRTNKILVTADSTVTGGKWDRLESTVQ
                     NLGAGTATIKKSAGTFKTFASGGDAIFAGGARCSLGFNVTAGDGSPAFLTAGHCGVAAE
                     AWSDSEAGEPIATVDQAVFPGEGDFALVKYDDPATVAPSEVDLGDRTVQISQAADATVG
                     TQVFRMGSTTGLADGQILGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGLALG
                     LTSGGSGDCTVGGETFFQPVTTALEAVGATLGDAGAGAGAGADEGVGAEDGVGGEEAGA
                     GAGEGADAGAGEGAGEGAAEEVGGQEVGGEAGAARRQVRRTPVRTQAEQPVPRTRTVTA
                     SRTTRG"
     misc_feature    complement(3935..3948)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.24"
                     /tool="antismash"
     misc_feature    complement(3942..3951)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     misc_feature    complement(4124..4144)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.43"
                     /tool="antismash"
     gene            4135..4548
                     /locus_tag="BM536_RS29050"
                     /old_locus_tag="BM536_028965"
     CDS             4135..4548
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676669.1"
                     /locus_tag="BM536_RS29050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_028965"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_073495706.1"
                     /transl_table=11
                     /translation="MTMTTTEADKILSDSFAPWVRALGLSVEALGDDRAILRLPWSTEL
                     AREGGGLSGQALMAAADTATVIAVSAARGAFVPMTTVQQSTSFQRAVTDSDVLIQAVLT
                     KLGRRMAFADVTMTAERSGELAARASTVYALLG"
     misc_feature    complement(4754..4769)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.45"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="BM536_RS29055"
                     /old_locus_tag="BM536_028970"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="BM536_RS29055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_028970"
                     /product="hypothetical protein"
                     /protein_id="WP_073495709.1"
                     /sec_met_domain="strepbact (E-value: 2.4e-36, bitscore:
                     118.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEIADADLDNVSGGLSVHALGTVTGLVDGIAPVSGL
                     VDTVVGTVEGATGLSTAPVTNLVAGL"
     gene            5365..6171
                     /locus_tag="BM536_RS29060"
                     /old_locus_tag="BM536_028975"
     CDS             5365..6171
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="BM536_RS29060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_028975"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_073495712.1"
                     /transl_table=11
                     /translation="MQFRQQALTKLQSPEELDLPVRFARPQGWLVLSVTVVVMAAASVW
                     AVTGSVASTVSAPAVLTHGEGSYILQSPAAGQVTAVLAKEGERLPAGSPVLKVRTADGE
                     TVVRTVAAGRITALAATIGQIIATGANVAAVEKVARADDPLYATVYVPAENAASVPVDA
                     AVDLTVQTVPSQRYGVLRGHVKSVDRTARSAQQISAFLGDSQLGEQFTEEGRPVAVLVR
                     LDRSSATESGYRWSSADGPPYALTSMTMATASIRLADQRPLDWLLP"
     gene            6270..8386
                     /locus_tag="BM536_RS29065"
                     /old_locus_tag="BM536_028980"
                     /pseudo=""
     CDS             6270..8386
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016325242.1"
                     /locus_tag="BM536_RS29065"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_028980"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 6.2e-36, bitscore:
                     117.5, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAVECGAASLAMVLGHHGRHVPLEELRIACGVSRDGSRASNLLK
                     AARGYGLTAKGMQMDLAALAEVRSPAILFWEFNHYVVYEGLGRRLGRRGVFVNDPAKGR
                     RFVPMEDFDGSFTGVVLVLEPGADFERGGRRPGVLGAMPSRLRGTAGTLPAAVLASLLL
                     VVVGAAVPALSRTYIDMFLIGGQTSLLEVLFASMGTCVLLTVALTWLQQANLHHGRIIS
                     STLSSARFLRHLLRLPVTFFSQRSPADLVQRLQSNDAVAETLARDLASAGVDAVVVVLY
                     AVLLYTYDPQLTFVGIGVALLNLVAMRVVIRMRATRTAKLRADNARLTNTAYTGLQLIE
                     TMKATGGEDGYFRKWAGQHATTLEEQQRLGVPGAWLGVVAPTLATVNSALILWIGGMRA
                     IEGAISVGLLVAFQALVARFTAPLTRLNGVAGRIQDFAADVARLKDVENFRADPLYDRR
                     SGGDATRRLHGHVELENIGFGYSPLDKPLLTGFDLSVGPGQQVALVGGSGSGKSTVSRL
                     ISGLYAPWEGVIRIDGQRLEDISRGALAASVSFVDQEVFLFEGTVRDNVALWDPSVPDE
                     AVVEALRDAPCTTSSCGGPAASTAGSSRTAATSPAVSANAWRSHGRWCAGPASWCWTR"
ORIGIN
        1 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nccccgccaa gccgcccccc cgcggtgggc gccaggtggg gtgctgtgct
      121 gccgggcgtc ggtccgccgc ccgcccggct gtccggtcgg gcggtcgccg caccggcacc
      181 ggccggaccc ccgccgtgcg cgcgcggccc gcagggggca gatttggttg cctgggatgg
      241 tgcggccggg cgaggaggtg gtcgccgtgt ccgacgagca gctgtccggc cgggcgcccg
      301 gggacccggc cggccggtcg atgctggccc cggcgttgac ggcgatgatg gacgccgtcg
      361 gggcgcactc cggcgcggtc tacctgctgc cggacgacga gccggtgctg gagatggcgg
      421 tgatggcggg actgccgcgg tcgttcgcgg cgccctggca gcgggtgggc gtcggcgcgc
      481 cgatcccgat cgcgcgcgcg gtgcgtgagc ggcggttggt gtgggtgggc ggcgaggagg
      541 agatggcccg ctcctatccg cggatcgccg tggtcctgcc gtacccgttc gcgctggccg
      601 cgctgccgct ggcgaccggc tcgaaggtgt acggcgcggt cttcgtgacc tggcccggct
      661 cgcacccgcc ggagctgtcc gaccgggaac ggcacgagct gcaggcggcc tgtgagcggc
      721 tggcgctgcg gctggagcgg gccgccgccg agaaccgtcc ggtgcgcggc gagagcgatc
      781 cgctggacac gcggccggcc gccgccgaca cgctgggcac ggtggaggcg gcgcggctgg
      841 tggcccggct gccgtacggg ctgtgttcgc tggacctgtc ggggcgggtg gggttcgcca
      901 acgccgccgc ggccgacctg ctcggggtcc cggtcggcga gctgctgggc acccacctgt
      961 gggcgtcggt gccctggctg gccgacccga tgtacgagga gcgctaccgg gccgcgctga
     1021 tcagccagca cgtcacgtcg ttcgtggcgc tgcgcccgcc ctcggcctgg ctgtccttcc
     1081 ggctgtaccc cagcaccacc gggatcagtg tgcgggtggt ccgggcccgg gcggtgtcgg
     1141 tgacgagccc ggccggtacg gagggcgacg acgccccgcc ccggctggtg tcgatgtcgc
     1201 aggcgctgag cctggccggg gcgctcaccg agacggtgag cgtgcaggac gtggtgcggc
     1261 tggtggccga ggagatcgcg ccggccgtgg gcagcggggc cctggcgctg ctcgggtcgc
     1321 gggcgggccg actgcacgtc ctgggacacc acggctaccc cgacccgcac ctggtggagc
     1381 ggttcgacgg gatgccgctg gcctccccca cccccggcac gcacgccgtc acccacggcg
     1441 tgcccgcgtt cttcgactcc cgccgccaac tggagcacct ctacccggcg cggcacgcca
     1501 cccccgacgg cttcgcggcc tgggcctacc tgccgctgat cgcctcgggc cgcccggtcg
     1561 gcgtctgcgt gctggcctat gccgagccgc acagcttctc cacgcacgaa cgcgccgtcc
     1621 tgaccagcct ggccgggctg atcgcgcagg ccctggaccg ggccctgctg tacgacgcca
     1681 agcaccggct cgcgcacggc ctccaggccg ccctgctccc ccactcgctg ccgtcgctgc
     1741 ccggcatcga ggcgagcgcc cgctatctgc cggccacccg gggcatggag atcggcggcg
     1801 acttctacga cctggtgccc tcgcgtccgc tgccggcggc ggtgatcggg gacgtacagg
     1861 ggcacaacgt gacggcggcc gggctgatgg ggcaactccg caccgccgtg agggcgtaca
     1921 cgaccgtggg gcagggaccg gccgaggtga tgcgcagcac caaccggctg atgatcgacc
     1981 tgggcacgga gctgttcgcc agttgcctgt atctgcggct ggacccggcg cacggtcgcg
     2041 cggtgatggc gcgggccggg catccgccgc ccctgctgcg gcggccggac ggacgggtgc
     2101 gggtcctcga cctggccggc ggcccgctgc tgggcatcga cgcggaggcg acgtatccga
     2161 ccacggagat cacgctgtcg cccggctgcg tactcgccct ctacaccgac gggttgatcg
     2221 agtcgccggg cgtggacatc gaggacgcgg tggcggacct ggggcggcgg ctgggcgagg
     2281 cgggcgatct gccgctggag gcgctggccg acgagctggt ggggcactac gcggctgcgg
     2341 aggagcggat cgacgacgtg gcgctgctgt tgctgcgggc ctcgtcggcg cgggaccggg
     2401 gctgacgcgc cgtcaacggc cgggctccgg ggaaacacgg acggtccggc cctcccgccg
     2461 gaggaccgga cgtggcaccg gccggacggg tgggtacgtc cggcggtggt acggggtgag
     2521 ctgtgcggac ggtgtgtcag tgggtctcgg tcagccccga gtcgtcctgg acgccgtcac
     2581 cgttctggtc ctcggcaccg gctgctccgc ctgcgtcctg accggtgtcc tcctgacctg
     2641 cctcctcgcc gcgccggcct cgccgccgac ctcctggccg ccgacctctt cagcggcacc
     2701 ttcaccggcg ccttcaccgg cgcccgcgtc ggcaccttca ccggcgcccg ctccggcctc
     2761 ctcgccgccg acgccgtcct cggcgccgac accctcgtcg gcacccgcgc cggctccggc
     2821 cccggcgtcg ccgagggtgg cgccgaccgc ttccagggcg gtggtgaccg gctggaagaa
     2881 ggtctcaccg ccgaccgtgc agtcgccgct gccgccggag gtcaggccga gcgcgaggcc
     2941 gtcctgggtg aacagcgagc cgccgctgtc gccgggctcg gcgcagacgt cggtctggat
     3001 gaggcccgtg acggtgccct cggggtagtt gacggtggcg tcgaggccga ggatctgccc
     3061 gtcggcgaga ccggtggtgc tgcccatccg gaagacctgg gtgccgacgg tcgcgtcggc
     3121 ggcctgggag atctgtacgg tccggtcgcc gaggtcgacc tcgctcgggg cgaccgtggc
     3181 cgggtcgtcg tacttcacga gtgcgaagtc gccctcgccc gggaagaccg cctggtcgac
     3241 ggtggcgatg ggctcgccgg cctcggagtc ggaccaggcc tcggccgcga cgccgcagtg
     3301 accggccgtc aggaaggccg gggagccgtc gcccgcggtg acgttgaagc cgagggagca
     3361 gcgcgctccg ccggcgaaga tggcgtcacc gccggaggcg aaggtcttga aggtgccggc
     3421 cgacttcttg atggtcgcgg tcccggcacc gaggttctgg acggtggact ccagccggtc
     3481 ccacttgccg ccggtcaccg tggagtcggc ggtgacgagg atcttgttgg tccgcgggtc
     3541 gaccgcccag gaggtgcccg ggatggtcgc gtcggccttg agggtctgcg cggcggcctt
     3601 gagttcggcc gtgctgttct cgacctcgcg taccttcgcg ccggccttct tggcctggac
     3661 gatgacgttg ttctggtcac cggagacgtc gacgacgttg acgacgagct ggttcttgcc
     3721 ggtgtcgaag taggagcccg cgaaggcgtc gccgagcagg ttctgcagct gcgaggcgag
     3781 gtccgaggcg tccgccgcct tcatggtcct cggcgccgcg gcggccgtgt cgccggcgtc
     3841 ctgggaggcg ttggcgtggg gcagcaggag tgccgccgct ccgagcgccg cgacaccgcc
     3901 caccgctatc gcggccttgc gcttcggaat tcgcttgtga ctcaaaaaac tcgacctcct
     3961 ggggggacgg ggtccggctg ccgggggcgg cagctccgta cagcggcgcc tggttggcca
     4021 tcagtacgca gggggtgcgg ggtgcgttca atcgcgtttg ccggcttcct ttgcgaaacc
     4081 gtgaatcggc catgaccgga gtccggggcc gtcggccggg gaacaatcgc ccatatgacg
     4141 atgacgacca ccgaagccga caagatcctt tccgactctt tcgcgccctg ggtgcgcgcg
     4201 ctgggcctgt ccgtggaggc gctgggggac gaccgggcga ttctgcgtct gccctggtcg
     4261 acggagttgg cgcgggaggg cggtgggctg tcggggcagg cgttgatggc ggccgcggac
     4321 accgcgacgg tgatcgccgt ctcggcggcc cggggggcct tcgtgccgat gacgacggtg
     4381 cagcagtcga cgtccttcca gcgcgcggtg accgattcgg atgtcctgat ccaggcggtg
     4441 ctgacgaagc tgggccgccg gatggcgttc gccgacgtca cgatgacggc cgagcgctcg
     4501 ggtgaactcg ccgcacgggc gagcaccgtc tacgccctcc tgggctgata ccgacggtga
     4561 ccggtcactg acggaccgtt cggatcggag agcggaatcc ctgcttcagg gaatctgcac
     4621 accgattgcc caagcgggtc acgggacacc gggcttctgc acaaccgcgc gtccccgccg
     4681 cgccattggg accggtgtgt cggattcgcc gcgagggcgt gcgccgctca tcggagcgat
     4741 ctcggcccga ggtgtgaaga agtcgccgac aaggcgtggc cgcgcctgca cgctttgact
     4801 ctcccgtgac gcaagtgccc tggtggggcc ggtgattgat tggaagcgtc ggcggcggac
     4861 ctgctttgat ccaacgcatc gcggggcccg ccggggcttt acggaaacgg gccttcggcg
     4921 cccgcggtcg cggccgtcat ctgtccccag agggggccgc tccccccttg tgagatatcc
     4981 atatgaaggg aagttccccc atgaactcca ccccccaggt cgagaccgtc gagatcgccg
     5041 acgccgacct cgacaacgtc tccggtggcc tgtccgtgca cgccctcggc accgtcaccg
     5101 gcctggtgga cggcatcgcc ccggtctccg gcctggtcga cacggtcgtc ggcaccgtcg
     5161 agggtgcgac cggcctgagc accgccccgg tcaccaacct ggtcgccggt ctctgatcgc
     5221 gcccttgtga ccctttgagt cccggagccg tcacccggtt ccgggactcc tcggtgccgc
     5281 gcgacccccg gcccggcccc gtgcaacccc ggctcgctcc cgtgaacccc cgagtcggtg
     5341 ccgtgaaccc ccgagggaag tcccgtgcag ttccgccaac aggccctcac caagctccag
     5401 tcacccgagg aactcgacct gccggtgcgc ttcgcccgcc cccagggctg gctggtgctc
     5461 tccgtgaccg tggtcgtcat ggccgccgcg tccgtgtggg cggtgaccgg ttcggtcgcc
     5521 tccacggtga gcgcgcccgc cgtcctcacc cacggcgagg gcagctacat cctccagagt
     5581 cccgccgccg gccaggtcac cgcggtcctc gcgaaggagg gtgagcggct gcccgccggc
     5641 tcccccgtgc tgaaggtgcg taccgccgac ggcgagaccg tggtgcgcac ggtcgccgcc
     5701 ggccggatca ccgcgctcgc cgccaccatc ggtcagatca tcgccaccgg cgcgaacgtc
     5761 gccgccgtgg agaaggtcgc gcgggccgac gacccgctct acgcgacggt gtacgtcccg
     5821 gcggagaacg cggcctccgt cccggtcgac gcggccgtcg acctgaccgt gcagaccgtg
     5881 ccgtcgcagc ggtacggcgt gctgcgcggc catgtgaagt cggtggaccg cacggcccgg
     5941 tcggcccagc agatctccgc gttcctcggc gacagccaac tgggcgaaca gttcaccgag
     6001 gagggccgcc cggtcgcggt cctggtccgc ctcgaccgct cctcggccac ggagagcggc
     6061 tacaggtggt cgtcggccga cggaccgccg tacgcgctca cctccatgac catggccacc
     6121 gcctcgatcc ggctggccga ccagcgtccc ctcgattggc tgcttccgtg accgcgaccg
     6181 agacccgcgg cagacggcgg gccgcgccgc ccaggcgtgc ggtccccaag agccgggtga
     6241 agaccgtccg cacgcccacc gtgctccaga tggaggccgt ggagtgcggg gccgcctccc
     6301 tcgccatggt cctcggccac cacggccggc acgtcccgct ggaagaactg cgcatcgcgt
     6361 gcggggtctc gcgggacggc tcgcgcgcga gcaacctcct gaaggcggcc cgcggttacg
     6421 gcctgaccgc caaggggatg cagatggacc tggcggccct cgccgaggtg aggtcgcccg
     6481 cgatcctgtt ctgggagttc aaccactacg tcgtctacga gggcctcggc cggcgcctcg
     6541 gccgccgggg tgtgttcgtc aacgaccccg ccaagggccg ccggttcgtg ccgatggagg
     6601 acttcgacgg cagcttcacc ggcgtggtgc tggtgctgga gcccggggcg gacttcgagc
     6661 gcggcgggcg caggccgggt gtgctgggcg cgatgccgtc ccggctgcgg ggcaccgcgg
     6721 gcaccctgcc ggccgccgtg ctggcgagcc tgctgctggt cgtggtgggc gcggccgtcc
     6781 cggcactgag ccggacctac atcgacatgt tcctgatcgg cggtcagacc tccttgctgg
     6841 aggtgctttt cgcgtcgatg ggcacctgcg tgctgctcac cgtcgcactg acctggctcc
     6901 agcaggccaa cctgcaccac ggccggatca tctcctcgac gctgtccagc gcccgcttcc
     6961 tgcgccatct gctgcggctg ccggtcacct tcttctccca gcgcagcccg gccgacctgg
     7021 tgcagcgcct ccagtcgaac gacgcggtgg ccgagaccct ggcccgcgac ctcgcgtcgg
     7081 cgggcgtgga cgcggtggtc gtcgtcctgt acgcggtcct cctctacacc tacgacccgc
     7141 agctgacgtt cgtcggcatc ggcgtggccc tgctgaacct ggtcgcgatg cgcgtggtga
     7201 tccggatgcg cgcgacccgc acggcgaagc tgcgcgcgga caacgcacgg ctgaccaaca
     7261 cggcgtacac cgggctccag ttgatcgaga cgatgaaggc gaccggcggt gaggacggct
     7321 acttccgcaa gtgggccggg cagcacgcca ccacgctgga ggagcagcag cggctcgggg
     7381 tgcccggcgc ctggctgggc gtggtcgcgc cgacgctggc caccgtcaac agcgcgctga
     7441 tcctgtggat cggcgggatg cgggcgatcg agggcgccat ctcggtgggt ctgctggtcg
     7501 ccttccaggc gctggtcgcc cgtttcaccg ccccgctgac ccggctgaac ggtgtggcgg
     7561 gccgcatcca ggacttcgcg gcggacgtgg cccggctgaa ggacgtggag aacttccggg
     7621 ccgacccgct ctacgaccgc cgctccggcg gcgacgccac ccgccggctg cacggccatg
     7681 tcgagctgga gaacatcggc ttcggttaca gcccactcga caaaccgctg ctgaccggct
     7741 tcgacctgag tgtcgggccg gggcagcagg tcgcgctggt gggcggctcg ggcagcggca
     7801 agtcgacggt gtcccggctg atctcgggcc tgtacgcgcc gtgggagggc gtgatccgca
     7861 tcgacggaca gcggctggag gacatctcgc gcggggccct cgccgcctcg gtctccttcg
     7921 tcgaccagga ggtgttcctc ttcgagggca cggtccgcga caacgtggcg ctgtgggacc
     7981 cctccgtccc ggacgaggcg gtggtggagg ccctgcggga cgcgccctgt acgacctcgt
     8041 cgtgcggcgg cccggcggca tccacagccg ggtcgagcag gacggccgca acttctccgg
     8101 cggtcagcgc caacgcctgg agatcgcacg ggcgttggtg cgccggccca gcatcctggt
     8161 gctggacgag gtgaccagcg cgctggacgc ggagaccgag caggtcgtca tggacaacct
     8221 gcgccgacgc ggctgcgcct gcgtggtcat cgcgcaccgg ctcagcacgg tccgcgacag
     8281 cgacgagatc gtcgtcctcg aacacggcac ggtcgtggag cgcggacggc acgaggacct
     8341 tctcgcacgc ggtggggcgt acgcggcgct ggtcagggag cggtgagatg acggccgtac
     8401 acgaaggcga cgtcgtcctc tcggcgttgg ggcggacggg cacccgggtc gactgcgccg
     8461 gggtcggccg gctggacctc gagggtccgc aggtgctgtg gctggtcgcc tccggcgcgc
     8521 tggacctgtt cgcggtggac gcggaacagc ggggccactg gcaccacctg ggccggctgg
     8581 aggcgggctc gctgctgctc ggcccggtcg ccgggccccg gcacaccctg gtggcccgcc
     8641 ccctgcgcga ctgcgtggtc caccgcgtcg gcctgcgcga gctgtaccag cccgcgagca
     8701 cccagacctg gtcctacgac gagtacggca accccctgta cgtgccgccc gcgtcgagcc
     8761 cgctggagta cgccctcgcc ctcggcgtcg gccgcggact gtccgtcctc ttccaggccc
     8821 cgatggccgg cgagcggccc gccgaggacg gggatgcccc ccgcgctcgt ccgttcgagc
     8881 gtgggggacg acgacgtgtt ctggatgcag gtcccgccgg gcagcgtcca gtacggctcg
     8941 ctgtacggcg cggaggccgc cgccgacctg ctgatggacc cggcggtgtg gcagtccatg
     9001 gtcgaccagc agtaccgcct gctgaccacc ctcgaccgct ggatcgagca gttggagcac
     9061 acccacgaga cgcgcacggc cgccggcatc aaggccggtg aggcggtgcg cgcccaggcc
     9121 gaccggacgc tgctggcgtc gatcggccgc aaggcggggc cgcgcgcgac ggccgcggac
     9181 gcggacgcca cgttcgccgc ctgcaagctg gtcgcggggg ccgccggcat cgccctcgtc
     9241 gagccgaccc ggacggcgcc ggcaacgacc gtctggaccc cgtcgagcag atcgcgctcg
     9301 cctcccgcgt gcgcacccgc gccgtacgac tggacggccg ctggtggcgc gacgacgtcg
     9361 gcccgctggt cgcacaccgg gcgctgtccg gggcgccggt cgcgctgctg tggcggcgcg
     9421 gcggctatgt cgccgtccat cccacgaccg gccgcgagac gccggtggag aaggccaacg
     9481 cggaggagtt cgagccgcgg gccgtcatgt tctaccgccc gctgcccgag cggccgctcg
     9541 gcccgctcgg gctgctgcgg ttcagcatgc ggggcaccgg ccgcgacctg acgaacctgc
     9601 tgctcggcgg gctcgtcacg gtggcgatcg gcgcgctggt gccggtcgcg accggcaagg
     9661 tgctcggcga gtacgtgccg cgggcccagc aggggctgat cgagcaggtg tgtctggccg
     9721 tcctggtcag cgccgtggtg gcggcggcct tcatgctcct ccagaacatg accatcctgc
     9781 ggctggaggg ccggatcgag gcgacactcc agccggccgt ctgggaccgg ctgctgaggc
     9841 tgccgacgaa gttcttcgcc gaacgctcca cgggtgagct ggcgagcgct gccatgggca
     9901 tcagcgccat ccgccgactg ctggcgggcc tcggcccgtc ggtcgctcag tccgtcacca
     9961 tcggcgccgt gaacctcggc ctgctgctct ggtacagcgt gccgatggcg ctggccgcga
    10021 tcgggatgct cgtcgtcgtc gccgccgtgt tcctggggct ggggctgtgg caggtgcgct
    10081 ggcagcgcag gctggtggtg ctctccaaca agctgaacaa ccaggcgttc cagaccctgc
    10141 ggggcctgcc caagctgcgc gtcgccgccg ccgagaacta cgcgtacgcc gcctgggccg
    10201 gccagttcgc ccgcag
//
