LOCUS       NZ_MPOH02000016        10849 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces phaeoluteigriseus strain DSM 41896 Scaffold_16, whole
            genome shotgun sequence.
ACCESSION   NZ_MPOH02000016
VERSION     NZ_MPOH02000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 632076)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Draft genome sequence of Streptomyces phaeoluteigriseus type strain
            DSM41896
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 632076)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2017) Strathclyde Institute of Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
REFERENCE   3  (bases 1 to 632076)
  AUTHORS   Schniete,J.K., Salih,T., Algora Gallardo,L., Martinez Fernandez,S.
            and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2016) Strathclyde Institute for Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2017
            Assembly Method                   :: SPAdes v. 3.9; MeDuSa v. 1.6
            Genome Coverage                   :: 65.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001896135.2-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,918
            CDSs (total)                      :: 7,837
            Genes (coding)                    :: 6,622
            CDSs (with protein)               :: 6,622
            Genes (RNA)                       :: 81
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1,215
            CDSs (without protein)            :: 1,215
            Pseudo Genes (ambiguous residues) :: 0 of 1,215
            Pseudo Genes (frameshifted)       :: 907 of 1,215
            Pseudo Genes (incomplete)         :: 407 of 1,215
            Pseudo Genes (internal stop)      :: 30 of 1,215
            Pseudo Genes (multiple problems)  :: 120 of 1,215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:00:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 541527
            Orig. end                         :: 552376
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MPOH02000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10849
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10849
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10849
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5849](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5849
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    166..180
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.56"
                     /tool="antismash"
     misc_feature    complement(379..391)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.33"
                     /tool="antismash"
     misc_feature    complement(1141..1155)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.07"
                     /tool="antismash"
     gene            complement(<1350..>1517)
                     /gene="aspA"
                     /locus_tag="BM536_RS43220"
                     /pseudo=""
     CDS             complement(<1350..1517)
                     /codon_start=1
                     /gene="aspA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670919.1"
                     /locus_tag="BM536_RS43220"
                     /note="catalyzes the formation of fumarate from aspartate;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="aspartate ammonia-lyase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLPTGQDFSAYTVVIEEERSRLAEAVGLAPGLDLGATATGTGLHA
                     PDGHAESVRRH"
     gene            complement(1801..2655)
                     /locus_tag="BM536_RS27075"
                     /old_locus_tag="BM536_026945"
     CDS             complement(1801..2655)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381901.1"
                     /locus_tag="BM536_RS27075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026945"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_342748842.1"
                     /transl_table=11
                     /translation="MRPVQFHLIGPFRITTDEGETFAPKAPKVCQMLAVLALQPREPVA
                     AETLVRELWGENPPSGAPRTLQTHVYHARRMLHDAQVTDDKRKLLLTQAPGYFLDLARD
                     EVDACVFEELVRLAQQELLQEAPERAAAHLDRALGLWRGPMLSNVPVAGVLSGRMAHME
                     ELRIRALELRVETQFRLGRYREILPELRILVNDHPLHEWFHGRLISALHRAGRRAEALQ
                     AYQNLYSVLRRELGLGPSEELQRLQADILNASTDGPLLHRCRPKSLSAPAEFTQLWDSA
                     VAS"
     gene            complement(2805..3974)
                     /locus_tag="BM536_RS27080"
                     /old_locus_tag="BM536_026950"
     CDS             complement(2805..3974)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988487.1"
                     /locus_tag="BM536_RS27080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026950"
                     /product="transposase"
                     /protein_id="WP_245873797.1"
                     /transl_table=11
                     /translation="MRLSLLKPVCTHMDSSCESLLEELGAHLFGSLQRSGQRLKAERYV
                     RGLLTVPGRKTLRNIAAQFEGGAAQQSVHHFISESPWEWMPVRHALAQYVQRNLAPDAW
                     VVRSTVIPKVGLHSIGVDQHFLPHMGQTVTGQQAVGAWLVSGRSAVPIDWRLRLSGRWL
                     SSPRRQRAGIPAGVTADSLDDCVREAVAAIAGIPQVRHRPVVVDVDGIDAPALARHFTS
                     AGVPFVVRVDPQEQLRIDRSQLPTYGDRVRTAAELASSLVSLRQQVNTGERPVTAVEIP
                     VTAAEPAAAGGGGMTLVGEWRGADTRPGKLWLAGGGLQPLPLVLWLTALPHVVARDFHA
                     ISDKVGIRDFAGRSFPGWHRHVTLASAAHLAVALEDSAVLPALHPAARA"
     misc_feature    complement(3264..3266)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3992..4007)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.78"
                     /tool="antismash"
     misc_feature    4061..4075
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            complement(4144..4803)
                     /locus_tag="BM536_RS27085"
                     /old_locus_tag="BM536_026955"
     CDS             complement(4144..4803)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155347.1"
                     /locus_tag="BM536_RS27085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026955"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073493443.1"
                     /transl_table=11
                     /translation="MARQERAIRTRRAILEAAARMFNELGYEASTIGMLIERAQLTRGG
                     VYFHFTSKEQLARAVLDEAVTREGLVPQALKLQEWVDLALLLAYRVPREPVLSASIRLS
                     VDSKARSLFGTRWPDWIAVSTDLLTEAKERGELRFHVDPAEIARLLVGAWTGVQLVTET
                     LPDRDLSQEVSALFALVLPSVVPAEVLAKLDISHYRAEHLLAAARSDEKSVAVPPS"
     misc_feature    4849..4863
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.96"
                     /tool="antismash"
     misc_feature    4914..4927
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.51"
                     /tool="antismash"
     gene            5001..5849
                     /locus_tag="BM536_RS27090"
                     /old_locus_tag="BM536_026960"
     CDS             5001..5849
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="BM536_RS27090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026960"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_158086546.1"
                     /sec_met_domain="AfsA (E-value: 3.4e-17, bitscore: 57.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLGAYTHLRHAEAVLVQSWRRVSENAFTLCLRWPAAPHGQPYDP
                     RILTQTIRQSGLTVAHAEYEVPLEHHTMLNSLDLTVSPDFRVSDTGPSTLVVDITVSPG
                     RSGRRAAHAMHMEIRTSPGGDPVVRADTEFAWISPAAYRRVRGDHLTVDWGDWPLPAPV
                     DAALVGRSRAADVVLAPTAVPHRWQLRNDVSNVLLFDHPVDHVPGLVLWEAAHQAVQAL
                     LAPVVSLPVSVTTTFLRYVEFGEPCLIDARVIGPAGPGGSPSGSRAPRPTRRPSRRTSV
                     R"
     gene            complement(6007..6771)
                     /locus_tag="BM536_RS27095"
                     /old_locus_tag="BM536_026965"
     CDS             complement(6007..6771)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (9-169). E-value: 2.5e-14. Score:
                     45.6. Matches aSDomain:
                     nrpspksdomains_BM536_RS27095_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988472.1"
                     /locus_tag="BM536_RS27095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026965"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_073493439.1"
                     /sec_met_domain="adh_short (E-value: 2.7e-37, bitscore:
                     123.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 4.9e-34, bitscore:
                     113.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSDLLKGKAVLVTGASSGIGEAAARVFAEEGAVVVVTARREERL
                     AALVGELRDKGAEAAYVVGDVRVAQDVARAVDFVVARYGRLDAAFNNAGIGGDRTPLHQ
                     MSDDVYDSVMDTNVRGVFNCLRHEIAAMLPNGGGSIVNNSSVGGLVAIPTAAPYIASKH
                     AVVGLTRAAADEYAKQGVRVNAIAPGTTRSEITLDWFSRNPGLQDLVNSLTPQGRTAEP
                     EEVAQAAAWLLSDRCPFLTGTVMPVDGGFVNQ"
     aSDomain        complement(6265..6744)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM536_RS27095_PKS_KR.1"
                     /evalue="2.50E-14"
                     /label="BM536_RS27095_PKS_KR.1"
                     /locus_tag="BM536_RS27095"
                     /protein_end="169"
                     /protein_start="9"
                     /score="45.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="AVLVTGASSGIGEAAARVFAEEGAVVVVTARREERLAALVGELRD
                     KGAEAAYVVGDVRVAQDVARAVDFVVARYGRLDAAFNNAGIGGDRTPLHQMSDDVYDSV
                     MDTNVRGVFNCLRHEIAAMLPNGGGSIVNNSSVGGLVAIPTAAPYIASKHAVVGLT"
     CDS_motif       complement(6652..6741)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM536_RS27095_0001"
                     /evalue="1.40E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="BM536_RS27095"
                     /protein_end="40"
                     /protein_start="10"
                     /score="14.6"
                     /tool="antismash"
                     /translation="VLVTGASSGIGEAAARVFAEEGAVVVVTAR"
     gene            6937..7311
                     /locus_tag="BM536_RS27100"
                     /old_locus_tag="BM536_026970"
     CDS             6937..7311
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577996.1"
                     /locus_tag="BM536_RS27100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026970"
                     /product="GNAT family protein"
                     /protein_id="WP_073493437.1"
                     /transl_table=11
                     /translation="MLGACPGDGAEPRPTRPWQAAEFGPRIHGIHLDGECAGGTCFALF
                     AAQLTTDRAVRLRGMSRVRCRVAPDDHAGIAVAERLGMPFEGVGRGHLPHRGRRPNPEV
                     RSVLAEERTAGPQRPAHIGT"
     misc_feature    7410..7425
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.09"
                     /tool="antismash"
     misc_feature    7421..7436
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.9"
                     /tool="antismash"
     gene            7460..8722
                     /locus_tag="BM536_RS27105"
                     /old_locus_tag="BM536_026975"
     CDS             7460..8722
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062: glycosyltransferase, MGT family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /locus_tag="BM536_RS27105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026975"
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="WP_342748843.1"
                     /sec_met_domain="MGT (E-value: 2.8e-116, bitscore: 384.0,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLAWALQAAGHDVRVASRPALAGPTTAAGLAAVSLVDENDLPRS
                     VGAATPAPEESRQAWSRITEAMAIDPVDPVAPHIWRLQRDYVAPALNDLQPADAPPDRP
                     QPVLDALVRFCRSWQPDLVLWDPTMPASAVAARTVGAAHARLLWGLDHVGWSVDRYARR
                     KDELGEALTDVDPVVAAVRAMAERYEMAFDDELLHGQWSLDPMPPGMRLPTGRRTVPMS
                     WVPYTGSGVLPEWLHERTGRPRVALTLGASMRAFSKDSKPLIANLLEAVGELDIDVVAT
                     LNAAQLEGIDRVPDNVRTIDYMPLSHLLPTCSAVIHHGGYGTMCAAAAAAVPQIVAMDG
                     DAVMEGPISAHYLTEQGAGLRLEHAECSVDDIRKMVIRVLEEPSFQRASEGLRRDLLAS
                     PSPADVVPVLERLVAEHRATV"
     misc_feature    8742..8759
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.14"
                     /tool="antismash"
     gene            8828..9700
                     /locus_tag="BM536_RS27110"
                     /old_locus_tag="BM536_026980"
     CDS             8828..9700
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988471.1"
                     /locus_tag="BM536_RS27110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026980"
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="WP_073493433.1"
                     /transl_table=11
                     /translation="MQKQPVTVIGLGPMGHAMASTYIDHGHRVTVWNRTASRADDLVAK
                     GAVLASSPAEALSASGLVVLSLTDYDAMYAILEPATHALSGKVVVNLSSDTPEKAREAA
                     KWAARHGAEYLTGGVQASPTGIGDPESFSFYSGPQQVFESVKETLEVLTKTDYRGEDPG
                     LAALYYQLQLDVFWTAALAYLHALAVADAHGISAQDMLPYLSGTVGSMPDFFAFYTPRI
                     DAGEHPGDVDRLAMGVASVDHVVQTAKDAGVDAALPEAVLEIFRRGVDAGHAADSFTSL
                     IETFRKPTA"
     misc_feature    9011..9013
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9762..10388)
                     /locus_tag="BM536_RS27115"
                     /old_locus_tag="BM536_026985"
     CDS             complement(9762..10388)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941194.1"
                     /locus_tag="BM536_RS27115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026985"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_073493432.1"
                     /transl_table=11
                     /translation="MSDEKRSADAPEPVVATRERIARAASRLMQRQGYDGTGIKQISAE
                     AGATLGSVYHFFPGGKLELALEALNRGNREFVDELRAVFDEEEDPAAAMVLLTRRLADG
                     LRASDWMDGCPITTTALGASGRLPQIQAAASEAFVRWHALVFDRLTESGVAEDAARALA
                     HTVISILEGAELTAQVTRSSAPLEAAGRHLARLIDGDREAATGQE"
     misc_feature    10388..10402
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.83"
                     /tool="antismash"
ORIGIN
        1 tgcgccgggt cgcccgcatc gaggaggtct accgcctcct ggaggtgctc agctcgatcg
       61 gcgtacgggc acgctggatc aacgagggtg tcgacctgga gatcgtgccg ccggccgaac
      121 tggacatggc ggcgatcgac gccgaggccg ccgtacgcac ccgctcgatc atcatgttct
      181 tcggtccgct gctgcaccgc atggaccagt tcaagctgcc gtacgccggc ggctgcgacc
      241 tcgggacgcg gaccatcgag ccgcacatga tcgcgctgcg ccggttcggg ctggacatcg
      301 cggcgacgga ggggcagtac cacgccgtcg tcgaccgtgc cgtccgcccg gaccggccga
      361 tcgtgctgac cgagcgcggg gacaccgtca cggagaacgc gctgctggcc gccgcccggc
      421 acgacggggt gaccgtcatc cgcaacgcct cctccaacta catggtccag gacctctgtt
      481 tcttcctgga ggcgctcggc gtcaaggtcg agggcatcgg caccacgacg ctcaccgtgc
      541 acggtgtgcc ggacatcgac gtcgacgtgg actactcccc ctccgaggac ccggtcgagg
      601 cgatgagcct gctggccgcc gccgtcgtca cggagtcgga gctgacggtg cgacgggtgc
      661 cgatcgagtt cctggagatc gagctggccg tgctggagga gatggggctc gatcacgacc
      721 gtacgccgga gtacgtcgcc gacaacggcc gtacgcgcct ggtcgacctc accgtccggc
      781 cgtcgaagct cgaggcgccg atcgacaaga tccacccgat gccgttcccc ggcctgaaca
      841 tcgacaacgt gccgttcttc gcggccatcg cggccgtcgc ccagggcaag accctcatcc
      901 acgactgggt ctacgacaac cgcgcgatct atctgaccga cctcaaccgc ctcggcggcc
      961 ggctccagct cctcgacccg caccgggtcc tggtggaggg cccgacccgc tggcgcgccg
     1021 ccgagatgat gtgcccgccg gcgctgcggc ccgcggtggt cgtcctgctg gcgatgatgg
     1081 cggcggaggg gacgtccgtg ctgcgcaacg tctatgtcat caaccgcggt tacgaggacc
     1141 tggccgagcg cctcaactcg atcggggcac agatcgagat cttccgggac atctgagccc
     1201 tcgtcgccga cgggccgccg cacatggctc gacctgcacg gacgcgggtc cgtcaaggtg
     1261 cggcggcagc cgggggacgt tcccgggacc tcgggccggg gcctggccgt cggcagtcgt
     1321 ccggtgctgg acggccgggg accgcgtcca tgacggcgaa ccgactcggc gtgtccgtcg
     1381 ggggcatgga gtccggtgcc ggtcgcggtg gcacccaggt cgagcccggg agcgagcccc
     1441 acggcctcgg cgagccggga gcgctcctcc tcgatcacga ccgtgtacgc ggagaagtcc
     1501 tgaccggtcg gcaacggggc ggggcaacgc cgtcgcgtca gcggtcgcgc cggccgcggt
     1561 gcgcagtgac gacgaccggc ggacggcggc gggacggcgg ggttcgccag gcgcttcgct
     1621 gccgtatccg gtcatgcgtg cgcgggcctt acgaggacag gcaccgtgcc gggcctgacc
     1681 gccccccggc gtacaccgcc acttcaccgg cgcgccccgt cgccggcgcc cgctcgctcc
     1741 tgcgcgtcgg gcggtgatcg gcggggaggc gtggccggcg acctctccgg tggtgcctcg
     1801 tcagctggcg acggcggagt cccacagctg cgtgaactcg gccggtgcgg aaagggactt
     1861 ggggcggcat ctgtgcagga gaggtccgtc ggtgctcgcg ttgaggatgt cggcctggag
     1921 ccgctggagt tcctccgagg gacccaggcc cagttcgcgc ctgagtacgg agtagaggtt
     1981 ctggtaggcc tggagggcct cggcccggcg gccggcgcgg tgcagtgccg agatgagccg
     2041 gccgtggaac cactcgtgca gggggtggtc gttgaccaga atgcgcagct ccgggaggat
     2101 ctcccggtag cggcccagcc ggaactgggt ctccacacgg agttcgaggg cgcggatcct
     2161 gagttcctcc atgtgggcca tgcggccgga gagcaccccg gcgaccggca cgttgctcaa
     2221 catggggccg cgccacagac cgagcgcccg gtcgaggtgc gccgccgctc tttccggtgc
     2281 ctcctggagg agttcctgct gggcgagtcg cacgagttcc tcgaagacac atgcgtcgac
     2341 ttcgtctcgg gcgaggtcga ggaagtagcc gggagcctgg gtgagcagca acttcctctt
     2401 gtcatcggtg acttgggcgt cgtggagcat acgacgggcg tggtagacgt gcgtctggag
     2461 ggtgcggggt gcgccgctcg gcgggttctc cccccagagt tcacggacca gcgtttccgc
     2521 cgcgacgggt tcgcgtggct ggagtgccag gacggcgagc atctgacaga ccttcggagc
     2581 cttgggagcg aacgtctcgc cttcatcggt agtgatccgg aatgggccga tgaggtggaa
     2641 ctgcactggc cgcacactcc ttccgccgcc gtccggcgct cgtggccgtg tccggtcggt
     2701 cgatccggac agctgagacg gtaggcggcg gaagactgtg agtcttctgt gcgaggccga
     2761 gagtgtcaca ttaccggacg gtaggttggt tactgtgggg cccgtcacgc gcgtgccgcg
     2821 gggtgcaggg cgggcagcac ggcgctgtcc tccagggcta cggccaggtg ggcggcggag
     2881 gccagggtga cgtggcggtg ccagccggga aaggaccggc cggcgaagtc ccgtatcccc
     2941 accttgtcgg agatcgcgtg gaagtcgcgc gcgacgacgt ggggcagcgc ggtgagccac
     3001 aggaccagcg gcagcggttg gaggccacca ccggccagcc acagcttccc gggcctggtg
     3061 tccgcccccc gccactcgcc gaccagcgtc atgccaccgc ctcccgcggc ggccggctcg
     3121 gcggcggtga ccggtatctc cacggccgtc acagggcgtt caccggtgtt gacctgctgc
     3181 ctcaggctga cgagggagga ggccagctcc gccgcggtac gcaccctgtc gccgtacgtc
     3241 ggcagctgcg accggtcgat ccgtaactgc tcctgagggt cgacccgcac gacgaagggg
     3301 accccggccg aggtgaagtg ccgggccagg gccggcgcgt cgatgccgtc gacgtccacc
     3361 accacgggac ggtggcggac ctgggggatg cccgcgatgg cggcgacggc ctcgcgcacg
     3421 cagtcgtcca gggagtcggc cgtgactccg gcggggatgc cggcccgctg ccggcgagga
     3481 ctgctgagcc agcggcccga cagccgcagg cgccagtcga tcggcaccgc ggacctgccg
     3541 gacaccagcc acgcccccac cgcctgctga ccggtcacgg tctgacccat gtgcgggagg
     3601 aagtgctggt cgacaccgat cgagtgcaga ccgaccttgg ggatcaccgt ggacctgacg
     3661 acccaggcgt ccggcgccag gttcctctgc acgtactgcg ccagtgcgtg gcgcaccggc
     3721 atccactccc acggcgactc gctgatgaag tggtggacgc tctgctgcgc cgcgccgccc
     3781 tcgaactggg cggcgatgtt gcgcagcgtc ttgcgccccg gcacggtcag caggccgcgc
     3841 acgtaccgct cggccttgag tcgctggcca ctgcgctgca gggatccgaa gaggtgagca
     3901 ccgagttctt ccagcaggga ctcgcaggac gagtccatgt gtgtgcagac gggtttgagc
     3961 aggctcagac gcacggatcc cccagttgac gtcggtcatg cgttcgacga ggtggcgcgt
     4021 aacgcacgca cccgcccgtc cactcttaca taaaccgtca atcagtcctg tttatttgcg
     4081 cgaagatcga ccgcctccgg gtgcccgcgg ccatcgacgt ccgccggagc cgccgacacc
     4141 gcgtcaggac ggcggaaccg cgacggactt ctcgtccgac cgggcggctg ccagcaggtg
     4201 ttcggcccgg tagtgggaga tgtccagctt ggccaggacc tcggcaggca ccacgctggg
     4261 cagcacgagg gcgaacaggg ccgacacctc ctgggacagg tcgcggtcgg gcagcgtctc
     4321 ggtgaccagc tggacaccgg tccaggcccc gaccaggagc cgggcgatct ccgccggatc
     4381 gacgtggaac cggagttcac cccgctcctt ggcctccgtg agtaggtccg tcgacacggc
     4441 gatccagtcc ggccaacggg tgccgaacag ggaccgtgcc ttggagtcga cggacagtcg
     4501 tatcgaggca ctgagcacgg gttcacgggg gacgcggtac gccagcagaa gcgccagatc
     4561 cacccactcc tgcagcttca gcgcctgcgg cacgagcccc tccctggtca cggcctcgtc
     4621 gagcaccgcg cgtgccagct gctccttcga cgtgaagtgg aagtacaccc cgccccgggt
     4681 gagttgggca cgctcgatga gcatgccgat ggtgctggcc tcgtagccca gctcgttgaa
     4741 catccgagcg gcggcctcga ggatggccct gcgtgtacgg atggcgcgtt cctgtctggc
     4801 cactcactcc accatgacct ttcctttact ggtgcttgaa ctcacactta ctcagtctcc
     4861 gaaaccggac ggagggccgg tactttaaca acgcaactca cgcagctgcc ttgtaggccg
     4921 gatccaaggg ggagatcttg cccgaagtcc ctgtagcgcc acgctgttta cccagccctc
     4981 ggggggaatc gccctcccgg ttgctcctcg gcgcctacac ccacctcaga cacgccgaag
     5041 ccgttctcgt ccagagttgg cggcgcgtga gcgagaacgc gttcacgctg tgcctccgtt
     5101 ggcccgccgc cccgcacgga cagccgtacg atccgcgcat actcacccag acgatcagac
     5161 agagcggcct gaccgtcgcg cacgcagagt acgaggttcc gctcgagcac cacaccatgc
     5221 tgaactccct cgacctcacc gtctcaccgg acttccgggt gtcggacacc ggtccttcga
     5281 cgctggtcgt cgacatcacc gtctccccgg gcaggagcgg ccgtcgtgcc gctcacgcga
     5341 tgcacatgga gatccggacg tccccgggcg gcgatcccgt ggtgcgggcc gacaccgagt
     5401 tcgcgtggat ctccccggcc gcgtaccgac gcgtgcgcgg cgaccacctc accgtcgact
     5461 ggggcgactg gcccctcccc gcccccgtcg acgccgcact cgtcggccga agccgcgccg
     5521 cggacgtggt gctcgcgccc acggccgtgc cgcaccgctg gcagctgcgc aacgacgtga
     5581 gcaatgtgct gctcttcgac caccccgtcg accacgtccc cgggctggtc ctgtgggagg
     5641 cggctcatca ggctgtccag gcgctgctcg ccccggtggt ctccctgccc gtttcggtca
     5701 ccaccacctt tctgcgctat gtcgagttcg gggagccgtg tctgatcgac gctcgggtga
     5761 tcggcccggc cggtccgggc ggttcgccgt ccgggtcacg ggcgcccagg ccgacgcgcc
     5821 ggccttcacg gcggacatcc gtacggtgac gcggccgatg ccgtgagact cccggaccgg
     5881 ggagtgcgcc gcggccaccg cggccggacg cactccccgg tccgctgtca ctgatcgacc
     5941 ggaggccgcc gagaccccgc cgtcgcccgc ccacagcggc ccgtcggccg gctccccggt
     6001 ccgctgtcac tggttgacga agccgccgtc caccggcatc accgtgccgg tcaggaaggg
     6061 acagcggtcg ctgagcagcc atgcggcggc ctgtgcgacc tcctccggct cggccgtacg
     6121 cccctggggc gtcagggagt tgaccagatc ctgaagccct gggttgcggc tgaaccagtc
     6181 gagggtgatc tcgctgcgtg tggtcccggg ggcgatggcg ttcacccgga caccctgctt
     6241 ggcgtattcg tcggcggcgg cccgggtcag cccgacgacc gcgtgcttgg acgcgatgta
     6301 cggggcggcc gtggggatgg ccaccagacc accgacactg ctgttgttga cgatggagcc
     6361 gccgccgttc ggcagcatgg cggcgatctc gtggcgcagg cagttgaaga cgccccggac
     6421 attggtgtcc atgacactgt cgtagacgtc gtccgacatc tggtgcaagg gggtgcggtc
     6481 accaccgatc ccggcgttgt tgaacgccgc gtcgagccga ccgtaccgcg cgacgacgaa
     6541 atccaccgcc ctcgctacgt cctgcgcgac gcggacgtcg ccgaccacgt acgccgcctc
     6601 cgcccccttg tcgcgcagtt cccccacgag agcggccagc cgctcctccc gccgagcggt
     6661 gaccacgacc accgccccct cctccgcgaa cacccgtgcc gccgcctcgc ctatgccgct
     6721 gctcgcgccg gtgacgagta cggccttccc cttcagaaga tcactggtca tggcgggagt
     6781 ctgacaggcg ggggcggcgg gcgtgattac cgcgcgatga ctgcgacctc gaccgcccgt
     6841 catacgctgc gacggctcgg tcccggcacg tgaggacgtg gccctgcggc ccgtcgtcca
     6901 gctcgtcggg aaaccacggc tccctaggag attgccttgc tcggtgcatg ccctggcgac
     6961 ggcgccgaac cgcgtcccac gcgaccgtgg caggccgccg agttcgggcc ccggatccat
     7021 ggcatccatc tcgacggcga gtgtgccggc ggcacctgct tcgccctctt cgccgcgcag
     7081 ctgacgaccg accgggcggt ccgcttgcgc ggcatgtccc gggtgcggtg ccgggtggca
     7141 ccggacgacc acgcaggcat cgccgtggcc gagcggctcg gcatgccctt cgagggtgtc
     7201 ggccgcggcc accttcctca tcgtggccga cgcccgaacc cggaggtccg gtccgtcctg
     7261 gccgaggaac ggacggccgg tccgcagcgc cccgcacaca tcggcacctg aacccgcggc
     7321 cctcctccgc tttccccggc tcgccaccgg ggtgagagcg gatgaggcgc gccatgccgt
     7381 cagtgagaga agagaatcgg agattcctgt gcgcgtcttg ttcaccgtct ggcccgctgc
     7441 ggcacatctc tttcccagtg tgccgctcgc ctgggccctc caggcagcgg gacacgatgt
     7501 ccgtgtggcc tcccggcccg ccctcgccgg tccgaccacg gccgccggac tcgccgccgt
     7561 ctcgctcgtc gacgagaacg acctcccgcg ttccgtcggt gccgcgactc cggctccgga
     7621 ggagtcgcgg caggcgtggt cgcgcatcac cgaggcgatg gcgatcgacc ccgtcgaccc
     7681 cgtggctccg cacatctggc gcctccagcg cgactatgtc gccccggcgc tgaacgacct
     7741 gcagcccgcc gacgccccgc ccgaccgtcc tcagcccgtg ctggacgcgc tggtccggtt
     7801 ctgccggagc tggcagccgg atctggtgct ctgggacccc acgatgccgg cctccgccgt
     7861 ggcggccagg accgtgggag ccgcccacgc acgcctgctc tgggggctgg accacgtggg
     7921 gtggtcggtg gaccgttacg cccggcgcaa ggacgagctg ggggaggcgc tcaccgacgt
     7981 ggacccggtg gtggcggcgg tgcgcgcgat ggccgaacgg tacgagatgg cgttcgacga
     8041 cgaactgctg cacggtcagt ggtccctgga tcccatgccg cccgggatgc ggttgcccac
     8101 ggggcgtcga accgtgccca tgagctgggt cccgtacacc gggtccggcg tcctgcccga
     8161 gtggctgcac gagcgcaccg gccgaccgcg cgtggccctc acgctgggcg cgtcgatgcg
     8221 cgccttcagc aaggacagca agccactgat cgccaatctc ctcgaagccg tcggcgagtt
     8281 ggacatcgac gtcgtggcca cgctgaacgc cgcacaactc gaggggatcg accgcgtgcc
     8341 cgacaacgtg cggacgatcg actacatgcc gctctcccac ctgttgccga cctgctcggc
     8401 ggtgatccac cacggcgggt acggcaccat gtgcgctgcc gcggctgcgg cggtgccgca
     8461 gatcgtcgcg atggacggcg acgcggtcat ggaagggccg atcagcgcgc attacctgac
     8521 cgaacagggc gcgggcctcc ggctggagca cgcggagtgc tcggtggacg acatccgcaa
     8581 gatggtcatc agggtcctgg aggagccgtc cttccagcgg gcctcggagg ggctgcgccg
     8641 tgatctcctc gcctccccga gcccagccga cgtggtgccg gttctcgaac gcctggtggc
     8701 ggagcaccgc gcaaccgtct gaacacggcg ccggttaggg ctggttagag cgttcttact
     8761 agcgcccgtg ctagcctgag cacttgttag aacgatcgat tcaatgggca gcgcgtaagg
     8821 ggtcttcatg cagaagcagc cggtcaccgt catcgggctg ggcccgatgg gccatgcgat
     8881 ggccagcacg tacatcgacc acggccaccg ggtgaccgtg tggaaccgca ccgcgagcag
     8941 ggccgacgac ctcgtggcca agggggccgt gctggcgtcc agcccggcgg aggcgctgtc
     9001 ggccagcggg ttagtggtcc tcagcctgac ggactacgac gcgatgtacg ccattctgga
     9061 acccgccacg catgcgctgt cgggcaaggt cgtcgtcaac ctcagctcgg acacccctga
     9121 gaaggcacgg gaggcggcca agtgggcggc acgccatggc gccgagtacc tcacgggtgg
     9181 cgtgcaggct tctcccacgg gtatcggcga ccccgagtcc ttctccttct acagcggccc
     9241 ccagcaggtg ttcgagtcgg tcaaggagac cctcgaggta ctcaccaaga ccgactaccg
     9301 gggtgaggac cccggcctgg ccgcgctgta ctaccagctc cagctggacg tcttctggac
     9361 cgccgcgctc gcgtacctgc acgcgctcgc ggtggccgac gcccacggga tcagcgcaca
     9421 ggacatgctg ccgtacctct ccggcacggt gggctcgatg ccggacttct tcgcgttcta
     9481 cacgccccgc atcgacgccg gcgaacaccc gggcgacgtc gacaggctgg ccatgggcgt
     9541 ggccagcgtc gaccacgtgg tgcagaccgc caaggacgcg ggcgtcgacg ccgcactgcc
     9601 cgaggcggtc ctggagatct tccggcgggg cgtcgacgcg ggacacgcgg ccgacagctt
     9661 caccagcctg atcgaaacct tcaggaagcc cacggcctga accgcttcga gggcgcgcgt
     9721 gcacaccgaa cggcgtgcgc gcgcgccctc ggcatgtcga ctcactcctg tccggtggcc
     9781 gcctctcggt ccccgtcgat cagccgggcc aggtgcctgc ccgcggcctc caacggcgca
     9841 ctgctcctgg tcacctgagc cgtcagctcg gcgccctcca agatgctgat gacggtgtgc
     9901 gccagtgcgc gggcggcgtc ctccgccacc cccgactccg tcagcctgtc gaagaccagg
     9961 gcgtgccatc gcacgaacgc ctccgacgcc gccgcctgga tctggggaag acggccggaa
    10021 gcgcccagtg ccgtggtggt gatggggcag ccgtccatcc agtccgacgc gcgcaacccg
    10081 tcggcgagtc tgcgggtgag gagcaccatc gccgcggccg ggtcctcctc ttcgtcgaag
    10141 acggcgcgca gttcgtcgac gaactcccgg ttgccgcggt tgagcgcctc gagggccagt
    10201 tccagcttgc cgccggggaa gaagtgatag accgagccga gcgtcgcgcc tgcctcggcg
    10261 gagatctgct tgatccccgt gccgtcgtat ccctggcgtt gcatcagtcg tgaggccgcc
    10321 cgggcgatgc gttcacgggt ggcgacgacg ggctcggggg cgtccgccga ccgtttctcg
    10381 tcactcatgg gcttcgtctt cacagtgcga ggctaccagg tagagcgttc tttccagagg
    10441 tggcgaccct catgatcccg agccggaatt accggaatta ccggctcatt gacggtgccc
    10501 cgatacggtc cgtgcgaacc gagcggtacg caatttcccc cgccgcaagg accgttgcag
    10561 agaaagaagg ccgaatggtc gccacggaga aggatttcct gaaggtgctc gaagcgattc
    10621 gagagatcgc tccgaccctc cgaggcaacg gcagacgcgc cgaggaggag aactggattc
    10681 cgcggaccaa catcgaactg ctggaaagca ccggcctgtt cggcgccgcg gtacccgagc
    10741 gcttcggggg tctcgatctg cccgccgagc agcaggccgc tctcctcacg gagacggcac
    10801 gagcctgcgg ctcgacgggc tgggtctccg tggtctgggt gacgacggc
//
