LOCUS       NZ_MPOH02000016        11331 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces phaeoluteigriseus strain DSM 41896 Scaffold_16, whole
            genome shotgun sequence.
ACCESSION   NZ_MPOH02000016
VERSION     NZ_MPOH02000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 632076)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Draft genome sequence of Streptomyces phaeoluteigriseus type strain
            DSM41896
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 632076)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2017) Strathclyde Institute of Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
REFERENCE   3  (bases 1 to 632076)
  AUTHORS   Schniete,J.K., Salih,T., Algora Gallardo,L., Martinez Fernandez,S.
            and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2016) Strathclyde Institute for Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2017
            Assembly Method                   :: SPAdes v. 3.9; MeDuSa v. 1.6
            Genome Coverage                   :: 65.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001896135.2-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,918
            CDSs (total)                      :: 7,837
            Genes (coding)                    :: 6,622
            CDSs (with protein)               :: 6,622
            Genes (RNA)                       :: 81
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1,215
            CDSs (without protein)            :: 1,215
            Pseudo Genes (ambiguous residues) :: 0 of 1,215
            Pseudo Genes (frameshifted)       :: 907 of 1,215
            Pseudo Genes (incomplete)         :: 407 of 1,215
            Pseudo Genes (internal stop)      :: 30 of 1,215
            Pseudo Genes (multiple problems)  :: 120 of 1,215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:00:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 482846
            Orig. end                         :: 494177
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MPOH02000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11331
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11331
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11331
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6331](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6331
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            303..491
                     /locus_tag="BM536_RS26840"
                     /old_locus_tag="BM536_026710"
     CDS             303..491
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="BM536_RS26840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026710"
                     /product="hypothetical protein"
                     /protein_id="WP_073493343.1"
                     /transl_table=11
                     /translation="MSDDNDDAYCLIDATRPPRAEGPPYAECVLCRKPTEYPESYKGIT
                     MCPVCEWQEAQRTACSG"
     gene            582..1283
                     /locus_tag="BM536_RS26845"
                     /old_locus_tag="BM536_026715"
     CDS             582..1283
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125241.1"
                     /locus_tag="BM536_RS26845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026715"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_245873781.1"
                     /transl_table=11
                     /translation="MSESPFQTEPHPRDEAPQFVLPLVVRLEKADPPGRTDALETAARA
                     VLVLLSDERALGDGEWAQTVRDWQDARIRKVVRRARGAEWRRAGELPGITVTGKAAEVR
                     VFAPVPLDGWPKELAKLQVSGTDLDDPEEPPAADPGVPVLWMSPEVEMSAGKAMAQAGH
                     GAQLAWWELSDEERSAWREAGFPLAVRTARPERWRELTTSGLPLVRDAGFTEIAPGSCT
                     VVAEHPALRSR"
     gene            complement(1329..2521)
                     /locus_tag="BM536_RS26850"
                     /old_locus_tag="BM536_026720"
                     /pseudo=""
     CDS             complement(1329..2521)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131257.1"
                     /locus_tag="BM536_RS26850"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026720"
                     /product="N(5)-(carboxyethyl)ornithine synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSLLSLGVLASSRKENEFRLPLHPAHLHRIAPDVRERIFLEQGYG
                     ERFGVADDALLPLVAGLRSREQLVAECDVMLLPKPMHEDVAELREGQVLWGWPHCVQDE
                     KMTQLAIDRRLTLIAWEAMNHWTSTGAFSVHVFHKNNELAGYCSVLHALQLGGVTGSYG
                     RRLRAVVISFGATARGAVTGLGAMGVSDVTVLTQRAAAAVASPMPSVVMGHFEEEEGDP
                     SRLRAFTAAGSVPLAEYLAGFDIIVNCVLQDTDAPLTFVTEEELALFRPGTFFVDVACD
                     EGMGFEWARRRPSATRCSPSGRTATTTRWITARPTCGTRPPGRSARHSCPTCARS"
     gene            complement(2578..2883)
                     /locus_tag="BM536_RS26855"
                     /old_locus_tag="BM536_026725"
     CDS             complement(2578..2883)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM536_RS26855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BM536_026725"
                     /product="hypothetical protein"
                     /protein_id="WP_073493341.1"
                     /transl_table=11
                     /translation="MYSQESISGHRRGRPEPTAETLSGLACLICATDYRDASAPEAVVV
                     SHRDDRQLLACRGTCARMAGGSVDGLDETPLPLDERVRRHQGPAPHDGDPHDGDPS"
     misc_feature    complement(3410..3423)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            3648..4763
                     /locus_tag="BM536_RS26860"
                     /old_locus_tag="BM536_026730"
     CDS             3648..4763
                     /EC_number="1.14.19.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381819.1"
                     /locus_tag="BM536_RS26860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026730"
                     /product="acyl-CoA desaturase"
                     /protein_id="WP_245873783.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 4.2e-20,
                     bitscore: 67.0, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIATAATDVDTDISHPGGPAGHPEMTKPPPVGRGSEYASLSREV
                     KQRGLLNRRPGYYSVKVGANLLLLAVGWTAFALIGQSWWQLVTAAFLAVMFTQTGFIGH
                     DAGHHQIATSKRVNTLLGHLHADLLIGLSYGWWIAKHNRHHAHPNHVDRDPDIADGAIA
                     FTQDQARVRHGAYAWLARRQAWLFFPMLLLEGLALHVAGVRAVFERGGTATSRRTRLVG
                     AALLTAHVGGYLAALFLVLSPVQAVCFLLVHQGLFGLYMGCSFAPNHKGMPIFGKDDRI
                     DFLRRQVLTSRNIRGGRVTDFALGGLNYQIEHHLFPSMPRPSLRHAQELVRTFCARHGV
                     AYHETGLVDSYAQVLRHLHAVGGPLRPELEY"
     gene            5001..6331
                     /locus_tag="BM536_RS26865"
                     /old_locus_tag="BM536_026735"
                     /pseudo=""
     CDS             5001..6331
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125240.1"
                     /locus_tag="BM536_RS26865"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026735"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 1.2e-97, bitscore: 321.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGTGIGWRPEIADAVERMPGIDWVEAVAENICPGHLPESLVR
                     LRERGVTVVPHGVSLGLGGADRPDEERLTALAERAEALGSPLVTEHIAFVRAGGPLTAT
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQESLPVPLAVENIAALLAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGLERDGVWHDSH
                     AHPVPRPVLDILTDLTSRVAPPGVLLERDENFPEPAELERELDAIDRRWRRGRGSGTRS
                     SGGRPPGRRRARVPTGGASRRPSPVTPRGSGSGSPRPPCCPRWSPARPSRRASTGCGSA
                     CRPARWPRSGLTWWPNWPRSCR"
     misc_feature    complement(6574..6590)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.2"
                     /tool="antismash"
     gene            6638..7420
                     /locus_tag="BM536_RS26870"
                     /old_locus_tag="BM536_026740"
     CDS             6638..7420
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073954.1"
                     /locus_tag="BM536_RS26870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026740"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_073493339.1"
                     /transl_table=11
                     /translation="MRPRPPIQGRGIFNGTGLIIAGLAVTLVALIVPVWSYEDRSATGV
                     DVLNAETVATLYGPLSGPDRDFVTKVRLAGLWELPAGRQAQAKGTTEAVRTAGRHLVEG
                     HAFLDERVRTVAAQLGLALPNEPTDQQQDWLKTLDAAQGEDFDREFANILRLAHGRVFS
                     LVAQVRAGTQNVLVRDLADDANTTVLDHIKVLEATGYVDFADLARDLAAGSTPPATRSP
                     APPGPVADPGAVVPLTPSPDPGYPLPPAATGPPTADSP"
     gene            7527..8290
                     /locus_tag="BM536_RS26875"
                     /old_locus_tag="BM536_026745"
                     /pseudo=""
     CDS             7527..8290
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983660.1"
                     /locus_tag="BM536_RS26875"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026745"
                     /product="TIGR04222 domain-containing membrane protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MFWVLLLLPAWAVAGVACTRLCLAAVRSASVDADAGQCHDLTLYE
                     AAFLSGGPGRVAELTLVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEGQSR
                     IAPVRAAAATADAVRGLADNLVGAGLAVPDGARTTVAAGVRQVRGAALTVFVLGATALL
                     MPTTSDLPRVLMAAWFGLPLALTLSCLALARIERTRTPAGPPRRDNACWPPWPGRRPRV
                     TTVPT"
     gene            8948..10545
                     /locus_tag="BM536_RS26880"
                     /old_locus_tag="BM536_026750"
                     /pseudo=""
     CDS             8948..10545
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (104-323). E-value:
                     1.8e-22. Score: 72.1. Matches aSDomain:
                     nrpspksdomains_BM536_RS26880_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133766.1"
                     /locus_tag="BM536_RS26880"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026750"
                     /product="alpha/beta hydrolase"
                     /pseudo=""
                     /sec_met_domain="PF00561 (E-value: 7.4e-27, bitscore: 89.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVLYSATGALLLTTLSAVPAGGTPNAVPGVVIAAERAAAEGV
                     DFGACTDVPDLPGSMECGKVGVPLDYARPNGRQIELAVSRVRATHRDPHNSKRRVPRQG
                     ALVFNPGGPGASGLYFPLIGMIPEWKRIGAAYDLVGYDPRGVGRSAPLSCEDPKTFFKG
                     PAPSPTHPSETYKRERVARAKAYARGCAQRAGDALRHYHSLNNARDLDVLRAALGEERL
                     TFMGASYGTYFGALYATLFPSHVRRMVFDAAVNPDPAQIWYRNNLDQSAAFESRWEDFR
                     EWVARHDRVYRLGGTSQAVLRSYERARAKLAAEPAGGKVGPAQLQGTFLQAGYYDDYWP
                     SRAEALSAYLEGDPKLLIALAAPPREAAAEAENANAVYTAVECNDGPWPTDFRVWDRDN
                     TRLARVAPFETWDNVWANLPCAYWRAPRQQPLDVRTGPGELRRR"
     aSDomain        9260..9916
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM536_RS26880_Abhydrolase_1.1"
                     /evalue="1.80E-22"
                     /label="BM536_RS26880_Abhydrolase_1.1"
                     /locus_tag="BM536_RS26880"
                     /protein_end="323"
                     /protein_start="104"
                     /score="72.1"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASGLYFPLIGMIPEWKRIGAAYDLVGYDPRGVGRS
                     APLSCEDPKTFFKGPAPSPTHPSETYKRERVARAKAYARGCAQRAGDALRHYHSLNNAR
                     DLDVLRAALGEERLTFMGASYGTYFGALYATLFPSHVRRMVFDAAVNPDPAQIWYRNNL
                     DQSAAFESRWEDFREWVARHDRVYRLGGTSQAVLRSYERARAKLAAEPAGGKVGPA"
     gene            complement(10546..11277)
                     /gene="hemQ"
                     /locus_tag="BM536_RS26885"
                     /old_locus_tag="BM536_026755"
     CDS             complement(10546..11277)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="BM536_RS26885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_026755"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_073493335.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNEVIRYTLWSVFKLKDTLPEDR
                     AGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTG
                     LGRALEPVWSNMALHRPAEFNRSHVPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKDIAELVAGLA"
     misc_feature    11269..11284
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.33"
                     /tool="antismash"
     misc_feature    11275..11290
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.49"
                     /tool="antismash"
ORIGIN
        1 gggcaggcgc tgcgcgcggt gcgcggcagc ggtgtcacgg cggtgcagtg ggacgtggtg
       61 agcggcgacg ccttcgccac cgacgcggac gcggtggccc ggcaggtgct ggagggcgtg
      121 cggccggggt cggtggtggt catgcactgc acgctcagcg ccgccccggt caccgagcgg
      181 gcggtgcgcg tgatcgtgcc cgagctgcgc cgccggggct accggttcgt caaggtgtcc
      241 gagctgatcg gcgccgtgga aggccgccgc tgaggcccgc gccgctctcg tacgctggag
      301 tcatgagcga cgacaacgac gacgcctact gtctgatcga cgcgaccagg ccgcccaggg
      361 ccgaggggcc gccgtacgcg gagtgcgtgc tgtgccggaa gccgacggag tacccggagt
      421 cgtacaaggg gatcacgatg tgcccggtgt gcgagtggca ggaggcccag cggacggcct
      481 gctcgggctg atcgccggga ggtgggcgca ccggccgccg ggggccgcgg ggccccggcc
      541 ggaccggccg gtccgttcgg cggtccagta ccctgcgccg agtgagtgag agcccgttcc
      601 agaccgagcc gcatccgcgt gatgaggcac cgcagttcgt ccttcccctc gtcgtccggt
      661 tggagaaggc cgatcctccc ggccgtaccg acgcgctcga gaccgccgct cgggccgtgc
      721 tggtgctgct gagcgacgag cgggccctcg gggacggcga gtgggcgcag accgtgcgcg
      781 actggcagga cgcccggatc cgcaaggtcg tacggcgggc ccgcggggcc gagtggcggc
      841 gggccgggga gctgcccggg atcaccgtga ccgggaaggc ggcggaggtg cgggtgttcg
      901 cgccggtgcc gttggacggg tggccgaagg agctggcgaa gctccaggtg tcggggaccg
      961 atctcgacga ccccgaggag ccgccggcgg ccgatccggg tgtaccggtg ctgtggatga
     1021 gccccgaggt ggagatgtcg gcgggcaagg ccatggcgca ggccgggcac ggcgcgcagc
     1081 tcgcctggtg ggagctgtcc gacgaggagc ggtcggcgtg gcgggaggcg ggcttcccgc
     1141 tggccgtgcg caccgcgcgg cccgagcgct ggcgcgagct gaccacgagc gggctgccgc
     1201 tggtgcggga cgcggggttc accgagatcg cgccgggcag ctgcacggtg gtcgcggagc
     1261 acccggcgct gcgctctcgg tgaggcctcg gggaggtgac cgggtctccg gtcacctccg
     1321 caggatcgtc aggccggccg gacggaggcc ttcgtcgccg gggtggcgag cggggccggg
     1381 gtctcggcgg cgtgcgggta ggcggccgcc cggttctgga aggacaggat cttcgggttc
     1441 atgacgacgc cgtcgcggat ctcgatggcc ttgccgaccg tggcgtcggc ctcccacgcc
     1501 gcggggccgc tcatgacctt gcgcaggtag ggcaggagtg cctcgctgat ctcccaggtg
     1561 gccgagttcc acaggtggga cgggctgtga tccaccgcgt agtagtggca gtcctgcccg
     1621 acggtgagca tcgggtcgcc gaaggtcgtc gccgggccca ttcgaagccc atgccctcgt
     1681 cgcaggcgac gtcgacgaag aaggtccccg gccggaacag ggccagttcc tcctcggtga
     1741 cgaaggtcag cggcgcgtcg gtgtcctgga ggacacagtt gacgatgatg tcgaacccgg
     1801 ccaggtactc cgccagcggc acggaaccgg ccgcggtgaa cgcccgcagg cgcgacggat
     1861 cgccttcctc ctcctcgaag tggcccatca cgaccgacgg catcggcgac gccaccgccg
     1921 ccgcggcccg ctgggtgagc accgtgacgt ccgagacgcc catggcgccc aggcccgtga
     1981 ccgctccgcg cgccgtcgcg ccgaagctga tgaccacggc ccgcaggcgg cgcccgtagc
     2041 tgccggtcac cccgccgagc tggagggcgt gcagcaccga gcagtagccg gccagctcgt
     2101 tgttcttgtg gaacacatgg acgctgaagg cgccggtgga ggtccagtgg ttcatggcct
     2161 cccaggcgat cagggtcagc cgtcggtcga tggcgagctg ggtcatcttc tcgtcctgca
     2221 cgcagtgcgg ccatccccac agcacctggc cctcgcgcag ctccgcgacg tcctcgtgca
     2281 tgggtttggg cagcagcatc acgtcgcact cggcgacgag ttgctcgcgg gagcgcagtc
     2341 cggccacgag cggcagcagg gcgtcgtcgg cgacgccgaa ccgttcgccg tacccctgtt
     2401 cgaggaagat cctctcgcgt acgtcggggg cgatccggtg gaggtgagcg gggtgcaggg
     2461 gcagccggaa ctcgttctcc ttgcgggagg aggcgagtac tccgaggctc aacaggctca
     2521 tgctcttcct catcatgtgg ttcttgtcgt gcggttcacg gctgccgggt ctccgggtca
     2581 ggaagggtca ccgtcgtgcg ggtcaccgtc gtgcggggcg gggccctggt gcctgcgtac
     2641 ccgctcgtcc agcgggaggg gcgtctcgtc gaggccgtcg accgacccgc ccgccatgcg
     2701 tgcgcacgtc ccccggcagg cgagcagctg cctgtcgtcg cggtgggaca ccaccaccgc
     2761 ctccggggcc gacgcgtcgc ggtagtcggt ggcgcagatc aggcacgcca gtccggagag
     2821 cgtctcggcg gtcggttcgg gacggccgcg tcggtgaccg gagatcgatt cctgtgaata
     2881 catgctccct gcatcctttt gatcggttcg gaacgtgcag gaggcgcggc gccggaatgc
     2941 ctctcgaacg ccccggaaac tgaggaaatc cgttaccgcg gggcgcggga acggcatggg
     3001 gagaacagcg cgtcagaaca ggccaggcag catcgtgcgg ctctttccgg ccggacgcgg
     3061 gggctccggc cttgccgttc cccgagggcg acgccgccgt ggatgaacga cacgcgaagt
     3121 gcgctcaatg ctgcaaccgt actccctccg ggcctccggc cgccctgtgc gaccgcccgc
     3181 cgggagccgc cgggaacgtt cccggcacac cggacgttga agtgagtggt tgtggttgaa
     3241 gtttgcgacg tggcagcgcc tgcggagttc tgacagcggg cgcttcgccg tttcgggggt
     3301 tttcttcgac ggctggcgac cggcagcccg ggaccgcgcg agtgcgcgtc ctcccaccta
     3361 ctcgccatct cgaaggagac ctctcgtggc tcgacgaacc gtgcggtggt tcatcccgga
     3421 caagggttcc ggcttcctcg ccgcctccgg ccaaggcccc gagcggctgc gggccgacca
     3481 ggtcagcgcc ttctgaggtt ccccgttccg agacaggccc catgccgagg gccggcctcg
     3541 cggcgcctgc gagcggcgcc gttcccggcg ccccacccgc acgccggaga cagcgctcgt
     3601 gcacgccgaa aggaaactct gtgaactccg cgaactccgt tacctccgtg acaatcgcga
     3661 ccgctgcgac cgatgtggac accgacatca gccatccggg cggtccggcg ggtcaccccg
     3721 agatgaccaa acccccgccc gtcggccggg gcagcgaata cgcctcgctg tcccgcgagg
     3781 tgaaacagag ggggctgctg aaccggcggc ccgggtacta ctcggtcaag gtgggggcga
     3841 acctgctcct gctggccgtc ggctggaccg cgttcgccct gatcgggcag tcgtggtggc
     3901 agctggtgac cgccgccttc ctcgcggtga tgttcaccca gaccgggttc atcgggcatg
     3961 acgccggaca ccaccagatc gccacctcca agcgcgtcaa caccctgctc ggccacctgc
     4021 acgccgacct cctgatcggc ctcagctacg gctggtggat cgccaagcac aaccggcacc
     4081 acgcccaccc caaccacgtc gaccgtgacc ccgacatcgc cgacggcgcg atcgccttca
     4141 cccaggacca ggcccgcgtc cgccacggag cgtatgcctg gctggcccgt cggcaggcgt
     4201 ggctgttctt cccgatgctg ctcctcgaag gactcgccct gcacgtggcc ggcgtacggg
     4261 cggtgttcga gcgcggcggc accgcgacct cgcggcggac caggctggtc ggggcggcac
     4321 tgctgacggc gcatgtcggc ggctatctcg ccgccctgtt cctggtcctg tcccccgtcc
     4381 aggccgtgtg cttcctcctg gtgcaccagg gactgttcgg gctgtacatg ggatgctcgt
     4441 tcgcccccaa ccacaagggc atgcccatct tcggcaagga cgacaggatc gacttcctgc
     4501 gtcggcaggt actgacctca cgcaacatcc gcggcggccg ggtcaccgac ttcgcgctcg
     4561 gcgggctgaa ctaccagatc gaacaccacc tgttcccctc catgccgcgc ccgagcctgc
     4621 gccatgccca ggaactcgtg cgcacgttct gcgcccggca cggcgtcgcc taccacgaga
     4681 ccgggctcgt cgactcgtac gcccaggtcc tgcgacacct ccacgcggtc ggcggcccgc
     4741 tgcgccccga gctggagtac tgatcgccgg tccggcccct tcggcggccc ccgtttcctc
     4801 tgtttccttc cctttctgtg ccgtttcttt gaaccgggcg ggccggccgc gcgtacctcc
     4861 aggcgccggg cgggccgtgc ggtgtgcgcg gccggaacct caaatgttgc tctgaaggag
     4921 caccgtcggc ggttcgggcc cggtcggcgg ggtcatatcc ccgtcaccac gcaggggggg
     4981 cggcgatgaa ggaggggacc atgacgcggc tgggtacggg aatcggctgg cgtccggaga
     5041 tcgcggacgc cgtggaacgc atgccgggca tcgactgggt cgaggccgtg gccgagaaca
     5101 tctgccccgg tcacctcccc gagtccctgg tgcggctgcg cgaacgcggg gtcaccgtcg
     5161 tcccgcacgg cgtctcgctc ggcctcggcg gcgccgaccg gcccgacgag gagcggctga
     5221 ccgcgctcgc cgagcgggcc gaggcactgg gctcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgggc gggcggcccg ctgaccgcca ccccacggct ggaggccggt cacctgctgc
     5341 ccgtgccgcg cacccgggac gccctggacg tgctgtgcga gaacgtgcgc atcgcgcagg
     5401 agtcgctgcc cgtcccgctc gccgtggaga acatcgcggc gctcctcgcc tggcccggtg
     5461 aggagatgac ggaggggcag ttcctgtacg agctggccga ccgcacgggc gtccggctgc
     5521 tcatcgacgt ggccaacctg cacaccaacc acgtcaaccg cggcgaggat cccgccaagg
     5581 cgctcgccga gctgcccctg gaggccatcg cgtacgtcca tgtcgcgggc ggcctcgaac
     5641 gggacggtgt ctggcacgac agccacgcgc acccggtgcc gcgccccgtc ctcgacatcc
     5701 tgaccgacct cacctcccgg gtcgccccgc ccggcgtgct gctggagcgc gacgagaact
     5761 tcccggaacc ggccgagctg gagcgggagt tggacgcgat cgacaggcgg tggaggcggg
     5821 ggcgcggcag cgggacacgg agcagcgggg ggcgcccgcc cgggagacgg cgggcgcggg
     5881 tgccgacggg gggagcgagc cgtcggccgt cgccggtgac gccacgcggc agcggctcgg
     5941 gctcgcccag gccgccctgc tgtcctcgct ggtcgccggc acgcccgtcc cggagggctt
     6001 cgaccgggtg cggctcggcg tgcaggcccg cgcgctggcc gcgaagcggg ctgacgtggt
     6061 ggccaaactg gccccggagc tgccgctgat cctcggcacg ggctaccggg ccgcgttcgt
     6121 cgcgtacgcc cagggccacc cgatgcgcgg cggttaccgg caggacgccc tggacttcgc
     6181 cgagcggctg ctgctcggcg ggcagttggc ggaggggcgg gcccggcgcg agctgcggcg
     6241 ctggtggctg gaccgctcgg gcccggcgcc gaggccgctg tcccggctgg gacgcgtggg
     6301 acaggtgggc cgggcgctga tcggtcgctg atcggtcgct gagcgggcac tgatcgggca
     6361 ctgatcgggg cgtgcccgcg gtgaactgcg cggtgagcca tggagtcgtc ccggcgttcg
     6421 agtgtgccgg ggttaacacc ggggtcggcc acacctcctc gccatccgtt ttccggtggt
     6481 ttccgctcat atggcatccg ctccggccca cccattcaac cctgtatgcc tgagatgcaa
     6541 cgcttcgccc gcgtacggca gtcggccccg ccgccgcagt aatatgccaa ctccgcaccc
     6601 gaagcacgag cgtgcggtgc cgtgagcagg aggcaccatg cgaccccgac ccccgatcca
     6661 gggcagaggc atcttcaatg gcaccgggct catcatcgcc ggcctggcgg tgacgctcgt
     6721 cgcgctgatc gtcccggtct ggtcgtacga ggaccggtcg gccaccggcg tcgacgtact
     6781 gaacgcggag accgtggcga ccctgtacgg gccgctgtcc gggcccgacc gcgacttcgt
     6841 cacgaaggtg cggctggccg gactctggga acttccggcc ggccggcagg cgcaggccaa
     6901 gggcacgacc gaggccgtac ggacggcggg gcggcacctc gtcgaggggc acgccttcct
     6961 ggacgagcgg gtgcgcacgg tcgccgcgca gctcgggctc gcgctgccga acgagcccac
     7021 cgaccagcag caggactggc tgaagacgct ggacgcggcg cagggtgagg acttcgaccg
     7081 tgagttcgcc aacatcctgc ggctggcgca cggccgcgtg ttctcccttg tcgcccaggt
     7141 gcgggccggc acgcagaacg tgctggtccg cgatctcgcc gacgacgcca acaccaccgt
     7201 cctggaccac atcaaggtcc ttgaggcaac cggttacgtc gacttcgccg acctggcccg
     7261 ggacctggcg gccggcagca ccccgccggc cacccgctcc cccgccccgc cgggccccgt
     7321 cgccgacccc ggcgcggtcg taccgctcac gccgtcaccg gacccgggtt acccgctccc
     7381 gccggccgcg accggtccgc ccacggccga cagcccctga cgcccagccg gacgaaggcg
     7441 aacggaacgc cgcataccca tgacgccggg tccggatagt gaacaaggca tggcgttctc
     7501 ctgcgtctcc ggcgtagaaa cgcggcatgt tctgggtcct tctcctgctg ccggcctggg
     7561 ccgtcgccgg tgtcgcgtgc acccggctgt gcctggccgc cgtccgttcg gcgtccgtcg
     7621 acgcggacgc gggtcagtgc cacgacctga ccctgtacga ggcggcgttc ctgtccggcg
     7681 ggcccggccg ggtcgccgaa ctgaccctgg tgtccatggc ccggcagcgg cggctgttgc
     7741 tcgcccacac cggctgggcg acggtcgtgg acccgcgcgg gcgcgacgag atggaacggt
     7801 ccgtcatagg agccatcggt cccgagggac agtccaggat cgctccggtg cgggccgccg
     7861 cggcgaccgc cgacgcggtc cgcggactcg ccgacaacct ggtcggcgcc ggcctcgcgg
     7921 tgcccgacgg cgcccgtacg accgtggcgg cgggcgtccg acaggtgcgc ggagcggccc
     7981 tgaccgtctt cgtgctgggg gcgacggcgc tgctgatgcc gaccacctcg gacctgccgc
     8041 gcgtcctcat ggccgcctgg ttcgggctgc ccctcgccct caccctgagc tgcctggccc
     8101 tcgcccgcat cgagcgcacc cgtactcccg ctgggcctcc ccggcgggac aacgcctgct
     8161 ggccgccgtg gcccggcagg cgcccgcggg tgacgaccgt acctacctga cctccatcgc
     8221 cgtccgcggc atccacgcgg cggccgaacc ggaactacgc gcggcgttcg cccaccggga
     8281 caggacatag gcgcggcacg gcgggccgtc gtcgccacga cgcgcggtga ggctcgtacg
     8341 acgcaggctg acgcaccgtc ggcggacggc gggccccacc cgacgggacg actgacgcac
     8401 ggcgagcccc ggccggcacc ggctgcccca cgaccgaccg cggccgcggc gggaacggca
     8461 ccggcgagca ccgcgccgaa acaggccgac gcccccacca cctcggcgga ccctcgaccg
     8521 aagggcagct gaacctcact gcgaaccctc gaccgaaggg cagctgaccc tcacggtggg
     8581 ccctcaccgg caccggcgcc ggtaccggta ccggtaccgc gaccgacccg cggccgatcc
     8641 cggccaccgt tcgaccgccg cctgccggcc cggaccggcg cgaccggtgc gccacggtcc
     8701 acggccggtc caacccccga aagcgaccga gccgacgcac ggccagggcg ccgtaagccc
     8761 acgatcggcc gcgcccggcc gacccacccc cacgcacgcc gaaccccgcc cgccgccggc
     8821 ggcgcgtccg ggtatcccgg gggcgcacag ggggcattgg cgccgacacc cctcggtggt
     8881 gcttgccttc atcaacaggc ggacgaacca tcccttctgt cgctgccgcg cttcgaaggg
     8941 acacgcgatg agagctgccg tcctctactc ggccacggga gccctgctcc tgaccaccct
     9001 ctccgccgtt cccgcgggcg ggaccccgaa cgccgtgccc ggtgtcgtga tcgccgccga
     9061 acgggcagcg gcggaggggg tcgacttcgg ggcctgcacg gacgtaccgg acctgccggg
     9121 cagtatggaa tgcggcaagg tcggtgtgcc gctggactac gcgcggccca acggcaggca
     9181 gatcgagctg gccgtcagcc gggtgcgggc cacccaccgc gacccgcaca acagcaagcg
     9241 ccgggtgccc cggcagggcg cgctggtctt caacccgggc gggccgggtg cctcaggcct
     9301 gtacttcccg ctgatcggca tgatcccgga gtggaagcgc atcggcgccg cctacgacct
     9361 ggtgggctac gacccgcgcg gggtcggccg ctcggccccg ctgtcctgcg aggatccgaa
     9421 gaccttcttc aaggggcccg ccccgtcccc gacgcacccc tcggagacgt acaagcggga
     9481 gcgcgtcgcg cgggcgaagg cgtacgcgcg tggctgcgcg cagcgggcgg gcgacgcgct
     9541 gcggcactac cactcgctga acaacgcccg cgacctggac gtcctgcggg ccgcgctcgg
     9601 cgaggagcgg ctgacgttca tgggggcgtc gtacggcacc tacttcgggg cgctgtacgc
     9661 gacgctgttc ccctcgcacg tgcggcggat ggtgttcgac gcggcggtga acccggaccc
     9721 ggcgcagatc tggtaccgca acaacctcga ccagtcggcg gcgttcgaga gccgctggga
     9781 ggacttccgg gagtgggtcg cgaggcacga ccgggtgtac cggctgggcg ggacctcgca
     9841 ggcggtgctg cgcagctacg agcgggcgcg ggcgaagctg gccgcggagc cggccggtgg
     9901 gaaggtgggg cccgcgcagt tgcagggcac gttcctccag gccgggtact acgacgacta
     9961 ctggccgtcc cgcgccgagg cgctgtcggc gtatctcgag ggcgatccca agctgctgat
    10021 cgcgctggcc gcgccgcccc gggaggccgc ggcggaggcg gagaacgcga acgccgtcta
    10081 cacggccgtg gagtgcaacg acgggccgtg gccgacggac ttccgggtgt gggaccggga
    10141 caacacgcgg ctggcgcgcg tggccccgtt cgagacctgg gacaacgtgt gggcgaacct
    10201 gccgtgcgcc tactggcggg cgccccggca gcagcccctg gacgtgcgga ccggtccggg
    10261 tgagctgcgc cgacgctgat cctggcggcc gaacgggacg ccgccgcacc ctacgacggc
    10321 gccctggaga tgcaccggcg gctgacgggc tcggtgctgg tgacggagcg ggacgccggc
    10381 acccacggca tcgcgggcgg gccgaaccgt tgcgtcaacg gccacctgga ggcgtatctg
    10441 ctggaagggc ggctgccggc gcggcgcgcg gcctgcgcgc cgcacgcgga gccccggccg
    10501 gccgaccgcg ccggcaaggc ggcggccgtc cggccgacgc gctgatcagg cgaggcccgc
    10561 gaccagttcg gcgatgtcct tgcggcggcc cgtgtagaac gggacctcct cgcggacgtg
    10621 catacgcgcc tcggaggcgc gcaggtggcg catgaggtcg acgatgcggt acagctcgtc
    10681 ggcctcgaag gcgaggatcc actcgtagtc gccgagggag aacgaggcga cggtgttggc
    10741 gcggacgtcc gggtagccgc gggccatctt gccgtggtcg gcgagcatgc ggcgacggtc
    10801 ctcgtcgggc agcaggtacc agtcgtagct gcgcacgaag gggtagacgc tgatgtagtc
    10861 gcgcggcgtc tcgtcggcga ggaacgccgg gacgtgcgag cggttgaact cggcggggcg
    10921 gtgcagcgcc atgttcgacc agaccggctc cagcgcgcgg cccagcccgg tgcggcggaa
    10981 gaggttgtag gcctcctgga gctggtcgct ggtctcggcg tgccaccaga tcatgaggtc
    11041 ggcgtcggcg cgcagaccgg acacgtcgta ggtgccgcgg accgtcacgt ccttggcggc
    11101 gagctggtcg aacagctcct ggacctcgtc ggcgtagccg gcgcggtcct cggggagggt
    11161 gtccttcagc ttgaagacgg accacagcgt gtagcggatg acctcgttga ggtccttggc
    11221 cagcttgccc ttgttcggga tcctgccgga ctcggtggtg ggggcgtcgt cactcatggg
    11281 tctcattctc ccgccccgcc gtgcagactt tgcacggggt gggccgtcag c
//
