LOCUS       NZ_MPOH02000015        10405 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces phaeoluteigriseus strain DSM 41896 Scaffold_15, whole
            genome shotgun sequence.
ACCESSION   NZ_MPOH02000015
VERSION     NZ_MPOH02000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 1501466)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Draft genome sequence of Streptomyces phaeoluteigriseus type strain
            DSM41896
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501466)
  AUTHORS   Salih,T.S., Algora Gallardo,L., Melo Santos,T., Filgueira
            Martinez,S. and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2017) Strathclyde Institute of Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
REFERENCE   3  (bases 1 to 1501466)
  AUTHORS   Schniete,J.K., Salih,T., Algora Gallardo,L., Martinez Fernandez,S.
            and Herron,P.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2016) Strathclyde Institute for Pharmacy and
            Biomedical Sciences, University of Strathclyde, 161 Cathedral
            Street, Glasgow G40RE, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2017
            Assembly Method                   :: SPAdes v. 3.9; MeDuSa v. 1.6
            Genome Coverage                   :: 65.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001896135.2-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,918
            CDSs (total)                      :: 7,837
            Genes (coding)                    :: 6,622
            CDSs (with protein)               :: 6,622
            Genes (RNA)                       :: 81
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1,215
            CDSs (without protein)            :: 1,215
            Pseudo Genes (ambiguous residues) :: 0 of 1,215
            Pseudo Genes (frameshifted)       :: 907 of 1,215
            Pseudo Genes (incomplete)         :: 407 of 1,215
            Pseudo Genes (internal stop)      :: 30 of 1,215
            Pseudo Genes (multiple problems)  :: 120 of 1,215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:00:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 787365
            Orig. end                         :: 797770
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MPOH02000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            1011..2111
                     /locus_tag="BM536_RS21505"
                     /old_locus_tag="BM536_021430"
     CDS             1011..2111
                     /NRPS_PKS="Domain: Aminotran_5 (74-337). E-value: 1.7e-26.
                     Score: 84.9. Matches aSDomain:
                     nrpspksdomains_BM536_RS21505_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="BM536_RS21505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021430"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_094103512.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.7e-21, bitscore:
                     70.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAARFASVEDRVARLLDTRQDVVIMQGEALLPLE
                     GAIRGTAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLAVPFHTAATADQIRAAFTEH
                     PEIGFVSLVHAEAATGNTNPVAEIGEVVKEHDALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPKAPRRSYLSLLDWKERWLDGGRKALPHAP
                     AQLEMLALEACVERIEADGLDTVMARHASAAAATRAGAVALGAGLEPYVHEAREAAPVA
                     TTLRAPAGVVASELVARALASDPALPLAAGGGALAKEMIRVNHYGADATPGAVRSSLTA
                     LGAALAERGLDVDLAGALRAGEQAWR"
     aSDomain        1233..2021
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM536_RS21505_Aminotran_5.1"
                     /evalue="1.70E-26"
                     /label="BM536_RS21505_Aminotran_5.1"
                     /locus_tag="BM536_RS21505"
                     /protein_end="337"
                     /protein_start="74"
                     /score="84.9"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAATADQIRAAFTEHPEIGFVSLVHAEAAT
                     GNTNPVAEIGEVVKEHDALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPKAPRRSYLSLLDWKERWLDGGRKALPHAPAQLEMLALEACVERI
                     EADGLDTVMARHASAAAATRAGAVALGAGLEPYVHEAREAAPVATTLRAPAGVVASELV
                     ARALASDPALPLAAGGGALAKEMIRVNHYGADATPGAVRSS"
     gene            2301..2813
                     /locus_tag="BM536_RS21510"
                     /old_locus_tag="BM536_021435"
     CDS             2301..2813
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140784.1"
                     /locus_tag="BM536_RS21510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021435"
                     /product="DinB family protein"
                     /protein_id="WP_073494139.1"
                     /transl_table=11
                     /translation="MTTLPDGRPVPLMTGDERAMLDSWLDFHRATLKLKCAGLDDTQVR
                     QASAEPSSLTLLGLVQHLAEVERNWFQRVFAGLDVPPVFENPTGYALSPERGLGEALTV
                     WQREIARGRELTAGRSLDDTGRIDGGPAAGAEVSLRWVLIHLVEEYARHNGHADILRER
                     IDGATGA"
     misc_feature    2918..2931
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.91"
                     /tool="antismash"
     misc_feature    complement(2941..2956)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     misc_feature    3048..3060
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    3051..3060
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.15"
                     /tool="antismash"
     gene            3085..3594
                     /gene="ectA"
                     /locus_tag="BM536_RS21515"
                     /old_locus_tag="BM536_021440"
     CDS             3085..3594
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381146.1"
                     /locus_tag="BM536_RS21515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021440"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_073494141.1"
                     /transl_table=11
                     /translation="MTATQADLRIDRPSAADGAALWRIAKESGTLDLNSSYSYLLWCRD
                     FAGTSAVARAESGEPVGFVTGYVRPQAPETLLVWQVAVDGAYRGRGLAATLLDALTARV
                     AAERPLTSVETTITPGNTASERLFTSYAARHGADVTREVLFDTGLFPDGPHDPEVLYRI
                     GPLTSV"
     gene            3670..4959
                     /gene="ectB"
                     /locus_tag="BM536_RS21520"
                     /old_locus_tag="BM536_021445"
     CDS             3670..4959
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.8e-73.
                     Score: 238.4. Matches aSDomain:
                     nrpspksdomains_BM536_RS21520_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="BM536_RS21520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021445"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_073494143.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3e-78, bitscore:
                     259.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPVVFDRAQGSRMYDEDGHQY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLMRDGVTHGLDMSTVAKRTFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRGRGEQIEQEL
                     ISITEENLADVKEYRGRGLVWGLEFHEKARAGRVARRAFELGLLIETSGPESEVVKLLP
                     ALTVTPEELDEGMSVLARAVRETVAVPETA"
     aSDomain        3754..4767
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM536_RS21520_Aminotran_3.1"
                     /evalue="3.80E-73"
                     /label="BM536_RS21520_Aminotran_3.1"
                     /locus_tag="BM536_RS21520"
                     /protein_end="366"
                     /protein_start="28"
                     /score="238.4"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHQYLDFFAGAGSLNYGHNNPVLKRALIDYLM
                     RDGVTHGLDMSTVAKRTFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRGRGEQIEQELISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="BM536_RS21525"
                     /old_locus_tag="BM536_021450"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="BM536_RS21525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021450"
                     /product="ectoine synthase"
                     /protein_id="WP_073494145.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.3e-54, bitscore:
                     178.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRSASGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDHETGLAHTITPGTMYLLDGHERHTLRVKEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5413..6315
                     /gene="thpD"
                     /locus_tag="BM536_RS21530"
                     /old_locus_tag="BM536_021455"
     CDS             5413..6315
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="BM536_RS21530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021455"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_073494147.1"
                     /sec_met_domain="PhyH (E-value: 6.8e-40, bitscore: 133.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTATGTHVTDLYPSRGATEVRVPRQDPVVWGAPDAPGPFEPLDL
                     QSFERDGFAAVDQLLTDDEVAVHKRELDRLVNDPAIRADDRSIVEPRTKEIRSVFEVHR
                     ISEVFARLVRDERVVGRARQLLGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGL
                     PRMRTVSVSIALTENHDTNGSLMIMPGSHKTFLGCAGATPKDNYKQSLQMQDAGTPSDE
                     ALTEMASAYGIKLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPF
                     AAPVRRPEFIGARDFTPVR"
     gene            complement(6421..7473)
                     /locus_tag="BM536_RS21535"
                     /old_locus_tag="BM536_021460"
     CDS             complement(6421..7473)
                     /NRPS_PKS="Domain: Aminotran_5 (17-344). E-value: 1.3e-23.
                     Score: 75.5. Matches aSDomain:
                     nrpspksdomains_BM536_RS21535_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="BM536_RS21535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021460"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_211362924.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.3e-18, bitscore:
                     63.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFETLVRAEFAPKNTYLNTASNGLLPARTVTALHEAALLRAEG
                     RPLTPLYEDVEASRAAYARLVGVPVTRVAAGASVAAHTGLIATSLPAGAEVLTAEDDFT
                     SVLNPFHVRGDLKVRAVPLERIAEAVRPGTALVAVSAAQSADGRIADLPALREAARTHG
                     ARTYVDFSQSAGWLPMDADAYDYSVSVSFKWLLGPHGAAFLVVPEDFGDLTPVLAGWVA
                     AEAPWDSCYGPVAELARSARRFDLSHALFTYAGLRRSLDLVEELGVAAVRAHDLALADR
                     FRAGLATLGHEPVPAPGSAIVSVPGLGVRQPELSRAGIEVSNRAGNLRASFHLFNTTAD
                     VDRLLDVLSG"
     aSDomain        complement(6442..7422)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM536_RS21535_Aminotran_5.1"
                     /evalue="1.30E-23"
                     /label="BM536_RS21535_Aminotran_5.1"
                     /locus_tag="BM536_RS21535"
                     /protein_end="344"
                     /protein_start="17"
                     /score="75.5"
                     /tool="antismash"
                     /translation="YLNTASNGLLPARTVTALHEAALLRAEGRPLTPLYEDVEASRAAY
                     ARLVGVPVTRVAAGASVAAHTGLIATSLPAGAEVLTAEDDFTSVLNPFHVRGDLKVRAV
                     PLERIAEAVRPGTALVAVSAAQSADGRIADLPALREAARTHGARTYVDFSQSAGWLPMD
                     ADAYDYSVSVSFKWLLGPHGAAFLVVPEDFGDLTPVLAGWVAAEAPWDSCYGPVAELAR
                     SARRFDLSHALFTYAGLRRSLDLVEELGVAAVRAHDLALADRFRAGLATLGHEPVPAPG
                     SAIVSVPGLGVRQPELSRAGIEVSNRAGNLRASFHLFNTTADVDRL"
     gene            complement(7587..8302)
                     /locus_tag="BM536_RS21540"
                     /old_locus_tag="BM536_021465"
                     /pseudo=""
     CDS             complement(7587..8302)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976951.1"
                     /locus_tag="BM536_RS21540"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021465"
                     /product="DsbA family oxidoreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALDAFPHRDQVEVVHRSFELDP
                     GRAKGDVQPVLAMLTKKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHFAKEQGRQDELIQLLYRANFAEERSLFGEGDERLVELAAEAGLDTDAARKVLATRPP
                     TPTRSAPTRPRPPGSARTASPSSSSTARTGSPAPSPPRSSPRHSPGPGASARPSSSSTR
                     ATPRPAAPTDAPYRGT"
     gene            complement(8463..9071)
                     /locus_tag="BM536_RS21545"
                     /old_locus_tag="BM536_021470"
     CDS             complement(8463..9071)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="BM536_RS21545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BM536_021470"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_073494151.1"
                     /transl_table=11
                     /translation="MDLELAELPFPLRTYGPDGHWSYEDGVLTGWGGPRQDRFVPPTGE
                     ALDPAADAPRLLGAPEGDFQFIARVTVGFNGAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFAVDGSSVWLRVSRTGRAFAFHASRDGEHWVFVRLFTLGDEKE
                     TGAALVGFMAQAPMGQGCVVTYDHLEFRPSWPDDLRDGS"
ORIGIN
        1 aggccgcggt cgcgtcgtac gggccgatcc actgctcgta gagcttcttg taggtgccgt
       61 cggccctggc gtcggcgatc gccttgttga tcgccgccac cagctgggtg ttgcccttct
      121 tcaccgtgaa gccgtactgc tcaccggtgt tgaggttgtc gacgacctcg aaggcgtcgg
      181 cgttcgcctt gtccttcagc cagccctgga cgaccgggta gtcgatgacg acggccttga
      241 cctggccggt gcgcaggccg ttgaggacgg cgtcggagga ctcgaaggag accgggtcga
      301 agccctgctt ggtcacgtag tcctcgccgg tggtctgcgc ctgcgccccg agcttgacgt
      361 tcccggactt gatctcggcg agggagtcca ccccggcgtc cttgttcacc aggacggcct
      421 gggtggcgtc gaagtagggg tcggagaagt cgacgttctt cttccgctcc tcggtgatgg
      481 tcatgccggc ggcggccagg tcgcactcgc cggagttcag gaaggcgccg gtcttgaagt
      541 tctcgaacgg cgtgtccagg atctcctgct tcacgcccag gtcgtcggcg accaggtcga
      601 tcacggagac gtcgaagccc tgcaccttgc cgtcgatctc cgactggaac ggcgggtagg
      661 gcaggtgggt gcaggtggtg agctgacccg ccttgaccag ctccacaccg cccgcggcgg
      721 tcttggagcc gctgccgccg tcgtcgctgg aggtacaggc ggccatgagc atgagcccgg
      781 ccgtagcggt ggtggcggcc aggacacggg tgcgacgccc gaggagcgtg ttcacggcgg
      841 gccttcctgt gggagaactg tagcttccga ttataaggag aagtttgagg ctctcaaatc
      901 aaacccttgg cagcggggtc cgtcgaccta agaagtcgcc ggtccggggt ggccggggtc
      961 acgccggtta ctctcgtcac ccttaccccg tgagtgaaga gagcaccgcc gtgacccacc
     1021 ccttcctgga tctcgctccg ctgagcgccg cccggttcgc ctccgtcgag gaccgggtgg
     1081 cacggctgct ggacacccgg caggacgtcg tgatcatgca gggcgaggcg ctgctgccgc
     1141 tggagggcgc gatccgtggg acggccgggc cgggcacgac cgcgctgaac gtgatcaccg
     1201 ggccgtacgg gcagaccttc ggcgactggc tgcgggactg cggcgccacg gtgatcgacc
     1261 tcgcggtgcc cttccacacg gcggccaccg ccgatcagat ccgcgcggcc ttcaccgagc
     1321 acccggagat cggcttcgtg tcgctggtgc acgccgaggc ggccaccggc aacaccaacc
     1381 cggtcgcgga gatcggcgag gtggtgaagg agcacgacgc gctgttctac ctggacgccg
     1441 tggcgtccgt gggcgccgag ccggtgctgc cggacgcgtg gggcgtggat ctgtgcgtga
     1501 tcggcgcgca gaaggcgatg ggcggtccgg caggcgtgtc ggcggtgtcg gtgagcgagc
     1561 gggcgtgggc ccggatggcg gcgaacccga aggcgccgcg ccgctcctac ctctcgctgc
     1621 tcgactggaa ggagcggtgg ctcgacggcg gccgcaaggc gctgccgcac gctccggctc
     1681 aactggagat gctggcgctg gaggcttgcg tggagcgcat cgaggcggac ggcctggaca
     1741 ccgtgatggc caggcacgcg tccgccgccg ccgcgacccg cgcgggtgcg gtggcgctgg
     1801 gggccgggct ggagccgtac gtgcacgagg cgcgggaggc cgcgcccgtc gccacgacgc
     1861 tgagggcgcc ggcgggtgtc gtcgcctcgg agctggtggc gcgggcgctg gcgagcgacc
     1921 cggcgctgcc gctggccgcg ggcgggggcg cgctggccaa ggagatgatc cgggtcaacc
     1981 actacggggc ggacgcgacg ccgggcgcgg tccgcagcag cctgacggca ctgggcgcgg
     2041 cgctcgcgga gcggggcctg gacgtggacc tcgccggcgc gctgcgagcg ggggagcagg
     2101 cctggcggtg aggagggcgc cgggtcggcg tgatggcggg cgggtcggcg gggtcgaggg
     2161 gcgggccggt cggcagggtc gcgctcgtgg ccgaggggca gggatggtcg gccgggtcgc
     2221 gctcgtggcc gaggggcagg gatggtcggc cgggtcgcac tcgtggccgg ggtgtcagtg
     2281 ggccctgcca tgctcgccgc atgaccactc tcccggacgg ccgtcccgtc cccctgatga
     2341 ccggtgacga gcgggccatg ctcgacagct ggctcgactt ccaccgcgcg acgttgaagc
     2401 tgaagtgcgc gggcctggac gacacccagg tccggcaggc ctcggccgag ccctcctccc
     2461 tcacgctgct cggtctggtc cagcatctcg ccgaggttga acggaactgg ttccagcggg
     2521 tgttcgcggg actcgacgta ccgcccgtgt tcgaaaaccc caccgggtac gcgctcagcc
     2581 ccgagcgggg actgggtgag gcgctgacgg tgtggcagcg ggagatcgcg cgggggcgcg
     2641 agctgaccgc ggggcgctcg ctggacgaca ccgggcggat cgacggcggg ccggcggccg
     2701 gggcggaggt cagtctgcgc tgggtgctga tccacctcgt cgaggagtac gcgcggcaca
     2761 acggccacgc ggacattctg cgggagcgta tcgacggggc caccggagcc tgaattcacg
     2821 gtaattccgc agttcctttc cccgcctttc tctcaccctt tcccaggatg ggtttcccga
     2881 gttctcctgc ccggatctcc cgggcccaaa cgcaaagatt tttcgagcac attacgagcg
     2941 cgtgacgcat tgcacagagg tagcgatttg ccccgtttta gccgatacaa aacgccacaa
     3001 accgacaccc gaccacgagg gcatcaccaa accatcgccg cctctccatg cattttttaa
     3061 tttccctcgg taagttcaat ccgcatgact gccacacaag cagacctgcg gatcgaccgc
     3121 ccctcggcgg cggacggggc cgctctctgg cgtatcgcca aggaatccgg gactctcgat
     3181 ctgaactcct cctacagcta cctgctgtgg tgccgggact tcgccggcac ctcggcggtg
     3241 gcgcgcgccg agagcggcga accggtcggg ttcgtcaccg ggtacgtccg tccccaggca
     3301 ccggagacgc tgctcgtctg gcaggtcgcg gtcgacggcg cgtaccgcgg ccgcggcctc
     3361 gccgccacgc tgctcgacgc gctgaccgcg cgggtcgccg ccgagcgccc cctcaccagc
     3421 gtcgagacca cgatcacgcc gggcaacacc gcctcggagc ggctgttcac ctcctacgcc
     3481 gcccgccacg gtgcggacgt cacgcgcgag gtcctgttcg acacgggact gttcccggac
     3541 ggcccgcacg atcccgaagt cctctaccgc atcggcccgt tgacctccgt ctgagccttc
     3601 cccggagacc cggcccccca ccgccgaccg ccgcacgact cccccaccgc atcgaggagc
     3661 gttttcgccg tgaccatcac ccagcccgac ctcagcgtgt tcgagaccct cgagtccgag
     3721 gtgcgcagtt actgccgcgg ctggcccgtc gtcttcgacc gggcgcaggg cagccggatg
     3781 tacgacgagg acggacacca gtacctcgac ttcttcgcgg gcgccggctc gctcaactac
     3841 gggcacaaca acccggttct gaaacgggcg ctgatcgact acctgatgcg cgacggcgtc
     3901 acccacgggc tcgacatgtc caccgtcgcc aagcggacct tcctccagac cttccaggat
     3961 ctggtgctgc ggccgcgcga cctgccgtac aaggtcatgt tcccgggccc gaccggtacc
     4021 aacgccgtgg agtccgcgct gaagctggcc cggaaggtga aggggcgcga ggcgatcgtg
     4081 tcgttcacca acgccttcca cgggatgtcg ctcggctcgc tcgccgtcac cgggaacgcg
     4141 ttcaagcggg ccggggcggg cgtcccgctg gtgcacggca cgccgatgcc gttcgacaac
     4201 tacttcgatg gccaggtccc ggacttcctg tggttcgagc ggctcctgga ggaccagggc
     4261 tccggactca acaagccggc cgccgtgatc gtcgagaccg tgcagggcga gggcggcatc
     4321 aacgtcgcgc ggccggagtg gctgcgggcg ctggccgagc tgtgcgagcg gcaggacatg
     4381 ctgctgatcg tcgacgacat ccagatgggg tgcgggcgca ccggtgcctt cttctcgttc
     4441 gaggaggcgg gcatcacccc cgacatcgtc accgtgtcca agtccatcag cggctacggc
     4501 ctgcccatgt cgctgtgcct gttcaagccc gagctggacg tgtgggagcc gggcgagcac
     4561 aacggcacct tccgcggcaa caacccggcg ttcgtcacgg ccaccgccgc gctggagacg
     4621 tactgggccg acgggtcggc gatggagaag cagacccgcg ggcgcggtga gcagatcgag
     4681 caggagctga tctcgatcac cgaggagaac ctggccgacg tcaaggagta ccggggccgt
     4741 ggcctggtgt ggggcctgga gttccacgaa aaggcgcgag ccgggcgggt ggcgcgccgc
     4801 gccttcgaac tcgggctgct gatcgagacg tccggcccgg agagcgaggt cgtcaagctg
     4861 ctgccggcgc tcaccgtgac gcccgaggag ctggacgagg gcatgagcgt cctcgcccgc
     4921 gccgtgcggg agaccgtcgc cgtgcccgag accgcctgac ctttccggca caccacctat
     4981 ccaggaggga tcgcaacacc gtgatcgtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtgag gtcggcgtcc ggcacctggg agagcaagcg catcgtcctc gccaaggaga
     5101 gggtcggctt ctccctgcac gagacgatcc tgtacgcggg cacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgcg tggagggcga ggccgaactc accgaccacg
     5221 agaccgggct ggcgcacacc atcacgcccg ggaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct ccgcgtcaag gaggacttcc gctgcatctg cgtgttcaac ccgcccgtca
     5341 cgggacggga ggaccacgac gagaacggcg tctatccgct gctcaccgag cccgaggagg
     5401 tgtgacatcc ccatgaccac tgccaccggc acccacgtca ccgatctcta ccccagccgc
     5461 ggcgccaccg aggtgcgtgt cccccgccag gacccggtcg tctggggcgc cccggacgcg
     5521 cccggcccgt tcgagccgct cgacctccag tcgttcgagc gggacggctt cgccgccgtc
     5581 gaccagctcc tcacggacga cgaggtcgcc gtccacaagc gggagttgga ccggctggtg
     5641 aacgacccgg cgatccgggc cgacgaccgt tccatcgtcg aaccgaggac gaaggagatc
     5701 cggtccgtct tcgaggtgca ccggatcagc gaggtcttcg cccggctggt gcgtgacgag
     5761 cgggtcgtcg ggcgggcccg gcagctcctc ggctcggacg tgtacgtcca ccagtcacgg
     5821 atcaacgtca agcccggctt cggcgcctcc ggcttctact ggcactccga cttcgagacc
     5881 tggcacgccg aggacggtct gccccggatg cgcacggtgt ccgtctcgat cgcgctgacc
     5941 gagaaccacg acaccaacgg cagcctgatg atcatgccgg gttcgcacaa gacgttcctg
     6001 ggctgcgcgg gggccacgcc gaaggacaac tacaagcagt cgctccagat gcaggacgcg
     6061 ggcacgccct ccgacgaggc gctgaccgag atggcgtccg cgtacggcat caagctgttc
     6121 acgggccggg ccgggtcggc gacctggttc gactgcaact gcatgcacgg gtccggcgac
     6181 aacatcacgc cgttcccgcg cagcaacgtc ttcatcgtgt tcaacagcgt ggagaacgcg
     6241 gcggtcgagc cgttcgcggc acccgtgcgg cggcccgagt tcatcggagc gagggacttc
     6301 acgccggtcc ggtgaacccg gccgcggtcc gggggggcgc ccccggacgt cacacggcat
     6361 cggcgcccgc gtcttcacgc ccggtgcacc gagcactccc gcccggcgca tccggcgtgc
     6421 tcagcccgac agcacgtcca gcagccggtc cacgtccgcc gtcgtgttga acaggtggaa
     6481 ggacgcgcgc aggttgcccg cgcggttgga gacctcgatg cccgcgcggc tcaactccgg
     6541 ctgccggacg ccgagtccgg gcaccgagac gatcgccgag cccggtgcgg gcacgggctc
     6601 gtggccgagt gtcgcgagcc cggcccggaa ccggtcggcg agggcgaggt cgtgggcccg
     6661 tacggcggcg acgccgagtt cctcgacgag atcgagggag cggcgcagcc cggcgtaggt
     6721 gaacagggcg tggctcaggt cgaaccgcct tgcggagcgg gcgagttcgg cgacgggtcc
     6781 gtagcagctg tcccagggcg cctcggcggc gacccatccc gccagtaccg gcgtgaggtc
     6841 gccgaagtcc tccggcacga cgaggaaggc ggccccgtgc gggccgagca gccacttgaa
     6901 ggagacggag acggagtagt cgtacgcgtc ggcgtccatc ggcagccagc cggcggactg
     6961 ggagaagtcg acgtaggtgc gcgcgccgtg ggtgcgggcc gcctcgcgca gggccggcag
     7021 gtccgcgatc cgtccgtcgg cggactgggc ggcgctcacc gcgacgagcg cggtgccggg
     7081 gcggacggcc tcggcgatcc gctccagcgg gaccgcgcgg accttgaggt cgccgcggac
     7141 gtggaaaggg ttcagcacgg acgtgaagtc gtcctccgcg gtgaggacct cggcgcccgc
     7201 cgggagcgag gtggcgatga ggccggtgtg cgcggcgacc gaggcgccgg ccgcgacccg
     7261 ggtgaccggg acgccgacga gccgggcgta cgccgcccgg ctggcctcca cgtcctcgta
     7321 cagcggcgtc agcggcctgc cctcggcccg tagcagcgcc gcctcgtgca gggcggtgac
     7381 ggtgcgggcg gggagcaggc cgttgctcgc ggtgttcagg taggtgttct tcggggcgaa
     7441 ctcggcgcgg acgagggtct cgaaggtctc catggcctcc actctgcggc ccacggaagt
     7501 cgaagtccat tgcgaattct tacgcggttt ccctaaggaa cgcttatgga tgggccctca
     7561 cctgcacgtt tcaggacccc ggcgggtcag tgccgcggta cggcgcatcc gtcggggccg
     7621 caggcctcgg cgtcgccctg gtcgacgagc tcgaggggcg agcgctcgcc ccaggcccgg
     7681 gtgagtgcct gggcgaagac ctcggcgggc tgggcgccgg agaccccgta cgtgcggtcg
     7741 aggacgaaga aggggacgcc gttcgcgccg agccgggcgg cctcggcctc gtcggcgcgg
     7801 acctcgtcgg cgtaggcggc cgggtcgcga ggaccttccg ggcggcgtcg gtgtccagac
     7861 cggcctcggc ggccagctcc acgagccgct cgtcgccctc gccgaacagg gaccgctcct
     7921 cggcgaagtt ggcccggtac aggagctgga tcagctcgtc ctggcggccc tgttccttgg
     7981 cgaagtgcag caggcggtgc atgtcgaagg tgttgccgtg gtcccggtcg cgggtgcggt
     8041 agtcgaggcc ctcggcggcg gcctgggctc cgaggttgtc ctcgccggcc tgcgcctggg
     8101 cctcgctcat gccgtacttc ttggtcagca tggccagcac gggctggacg tcgcccttgg
     8161 cgcggccggg gtccagttcg aaggagcggt gcacgacctc gacctggtca cggtgcggga
     8221 aggcgtcgag cgccttctcg aagcgggcct tgcccacgta gcaccacggg caggcgatgt
     8281 cgctccagat ctcgacgcgc atgtctcggc tctctcaggt atgcgggtac ggagactccc
     8341 tccgccgagt atgtgaacgt tcaaaggatc ggcctcattc cccgctcgcg gacaccgcgg
     8401 gaaaggtggt gatcgccgga taccacggcc cgcccgtcgc accggttccg gagcggcccc
     8461 ggtcagctgc cgtcgcggag gtcgtccggc cagctcgggc ggaactccag gtggtcgtac
     8521 gtgacgacgc agccctgccc catcggcgcc tgcgccatga agccgacgag ggccgcgccc
     8581 gtctccttct cgtcgcccag cgtgaacagc cggacgaaga cccagtgctc tccgtcgcgc
     8641 gaggcgtgga aggcgaaggc ccggccggtc cggctgaccc gcagccagac cgaactgccg
     8701 tccacggcga aggagttggc gtcgtcggag tggccgcggg tgaccaccgt gcagacggtg
     8761 ggcacgtccg gggagtactc caggcacagc ttggcccagg cccgttcgcc gacgtgcacg
     8821 tacaacaccc cggcgtcgaa ggcaccgttg aagccgacgg tcacccgggc gatgaactgg
     8881 aagtcgccct cgggtgcccc cagcagccgg ggggcgtcgg cggccgggtc cagcgcctcc
     8941 ccggtgggcg gcacgaaccg gtcctggcgg ggaccgcccc atccggtgag gaccccgtcc
     9001 tcgtagctcc agtgcccgtc gggcccgtag gtgcgcagtg ggaaggggag ttcggcgagt
     9061 tcgaggtcca tgctcagggt ttcccggatt ccttctcgtc gatcggtgcc ggtcagcgct
     9121 cgaggtggtt gttgaagcgc cggggcaggc cgagggggtt gtcgtcgcgc agctcgggcg
     9181 gcagcagggc ctcgggggtg ttctggtagg cgaccgggcg cagccagcgc tcgatggccg
     9241 taccgcccac ggaggtggag gtggaggtgg tcgccgggta ggggccgccg tggtgctgcg
     9301 cgggggccac ggccacgccg gtcggccagc cgttcaccag cacgcggccc gcgagcgggg
     9361 tgagttcggc gaggatctcg gcgccgcggc cctcgccggc ggcctcctgc gtcgacaggt
     9421 ggacggtggc ggtgaggttg ccggggagcc gggagagcac ggaccgcgcc tggtcgtcgt
     9481 cgtcgtagcg cgccaccacg gtcaccgggc cgaagcactc ctccaggagc aggtcgtacg
     9541 ccccctcgtc ggcgagcctc ccggccggga ccgtcaggaa gcccgcgctc acggtgtgct
     9601 cgccgcccgc tccgggcgtg accggcgtct cgacctcggg cagctgcgcc cgctcggcga
     9661 caccggcgag gaagttgtcg cgcatgcggt ggtcgagcag gacgcccgcg tccgtgtcgc
     9721 tgaccgcgtc ggtcagcgac ttgacgaggc cgtcaccggc cgcgccggac ggcacgagga
     9781 ccaggcccgg cttcacgcag aactgtccga cgcccagcgt catcgagccg gccaggccgg
     9841 tcccgatggc ctccgcgcgc tcggcggcgg cggcctcggt cacgacgacc gggttgagcg
     9901 agcccagctc gccgtggaag gggatcggca cgggccgcgc ggccgccgcg tcgaacagcg
     9961 cgcgaccgcc ccggatggac ccggtgaaac cggcggcggc gatcagcggg tgcttgatca
    10021 gctcgacgcc cgcctcgaag ccgtgcacca gaccgagcac ctcgacgggg atgccgtgct
    10081 cggcggcggc ccggcggacc accttggcga ccagctcgga gaggcccggg tggtcggggt
    10141 gggacttgac gacgacgggg ttgcccgcgg ccagcgcgct cgcggtgtcg ccgccgacga
    10201 cggagaacgc gaaggggaag ttggacgccg agtagacggc gacgacgccc agcggcacct
    10261 tgtagcggcg caggtccggc accggcggcg tcgcggtgtc gtcggggtgg ttgatgacga
    10321 cgtcgaggaa ggcgccctcc tcgacgatgt cggcgaaggc gcgcagctgg tagcaggtgc
    10381 gggcgagttc gccggtcagc cggac
//
