LOCUS       NZ_MOEO01000027         9758 bp    DNA     linear   CON 22-AUG-2024
DEFINITION  Bacillus sp. FMQ74 NODE_27_length_9758_cov_132.25, whole genome
            shotgun sequence.
ACCESSION   NZ_MOEO01000027
VERSION     NZ_MOEO01000027.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. FMQ74
  ORGANISM  Bacillus sp. FMQ74
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Okshevsky,M., Marshall,I., Schreiber,L. and Meyer,R.L.
  TITLE     Draft Genome Sequence of Bacillus sp. FMQ74, a Dairy-contaminating
            Strain Isolated from Raw Milk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Okshevsky,M., Marshall,I., Schreiber,L. and Meyer,R.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) Bioscience, Aarhus University, Ny Munkegade
            116, Aarhus 8000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2016
            Assembly Method                   :: SPAdes v. 3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001875785.1-RS_2024_08_22
            Annotation Date                   :: 08/22/2024 02:46:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,310
            Genes (coding)                    :: 4,147
            CDSs (with protein)               :: 4,147
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1 (5S, 23S)
            partial rRNAs                     :: 2, 12, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 105 of 163
            Pseudo Genes (incomplete)         :: 96 of 163
            Pseudo Genes (internal stop)      :: 20 of 163
            Pseudo Genes (multiple problems)  :: 53 of 163
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:13:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9758
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOEO01000027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9758
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>9754](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>9754)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..6110)
                     /locus_tag="BLL41_RS21925"
                     /old_locus_tag="BLL41_21640"
     CDS             complement(<1..6110)
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-30). E-value: 2e-18.
                     Score: 57.3. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21925_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-304). E-value:
                     3.5e-117. Score: 382.7. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21925_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (468-865). E-value:
                     2.8e-104. Score: 340.9. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21925_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (971-1040). E-value: 4e-23. Score:
                     73.4. Matches aSDomain: nrpspksdomains_BLL41_RS21925_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1054-1333). E-value:
                     6.6e-102. Score: 332.5. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21925_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1499-1901). E-value:
                     9e-114. Score: 372.2. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21925_AMP-binding.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="BLL41_RS21925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21640"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_141770224.1"
                     /sec_met_domain="PP-binding (E-value: 2.4e-16, bitscore:
                     47.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1e-87, bitscore:
                     281.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.9e-121, bitscore:
                     391.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFAIVGKV
                     NPQYFQNSINALVARHDIFRTIFISQNVSSPQQVVLRERNVIVQEKDITHLNEEKQSQF
                     IEQLKEKDRAQGFHLQKDLLMRITLIQTGEGQYTCIWTFHHIMMDGWCLSIVLKEFLHI
                     YASYVNESAISLEPVQPYGRYIKWLMEQDKKQAVSYWDHYLSGYEQQTMLPKQKKTKGR
                     SRQEHVTFSFSKKESSRLSELAAREEVTLSTIFHTIWGILLQKYNNHDDAVFGSVISGR
                     PAEIEGIEHMVGLFINTIPVRMQGAKTPFLQLIKDMQKDRLAAEAYSYHPLYEIQARSA
                     VKQGLIDHILVFENYPVQQEIQMLNKQEHASDLFQIHNFTVADETNYSFYLMVAPGEEI
                     HMKMCYDAEQHDRSFVLSVKEHLLNVVSQILHNPNLPPGEIEITTDTEKRQLIGEIADR
                     TPVYETIHAMFETQAEKTPDAYAVIDQACSLTYKELNKSANRLARHLRMKGVARQEPVA
                     IMIERSAAFITGVLGILKAGGAIVPIDPYYPADRIRYILNDCGCSHVVSQAHLSSSLEN
                     NYIITHAEDIESKEDGSNMKSVNNADDLLYIIYTSGTTGKPKGVQFEHRNMANLLKFEY
                     VHSGIDFEADVLQFATPAFDVCYQEIFSALLKGGTLHIVPEAIKRDVPQLFTFINKHQT
                     NIVFLPTAFIKMIFSERELANSFPYGVKHLIAAGEQLMISDLFQDVLRERGIHLHNHYG
                     PSETHVVSTYTIQPGDPIPKFPPIGRPIGCTDLYILNHQKQLQPYGVPGELYISGASVA
                     RGYVNYDKLTRDKFSSDPFRPGAIMYRTGDLARRLEDGNIEYIGRADNQVKIRGYRIEP
                     QEIEVTLMNHPDMKEAAILIRQDQNGEHELCAYYCSVQKLNAIDLRSYMASELPEYMIP
                     AKWVWVDSIPLTPNGKVDQSALPEPDASISGNPYTAPRNLLEAKLSQLFEDVLKNGSIG
                     IQDNFFDHGGHSLKATVLMSRIAKEFHVQVPLKDIFAYPTVEGLASIIRNAEENPYAAI
                     EPAEERAAYPVSSAQKRIYVLQQLDDGVAYNMPAVLELEGTLDVANLSAVCKELISRHE
                     PLRTSFVSGADGEPVQRIHTEVPFELSRETDIERFIRPFDLSRAPLFRAGLVKVSNEKH
                     VLLVDMHHIISDGVSVQLLIREFTDLYAKRQLKPLRIQYKDYAVWQQLFKKGDSYQKQE
                     TYWLQQFSGDLPVLELPTDKRRPAERKFSGGKVTFQMDKEITAKIKRLAHKNKSTLYMA
                     LLALYSAFLSRLSAQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRPAGNKTFEEFL
                     KEVRQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVLQNMERQKLKMNDVQLQ
                     WNDLEHPISKFDISLYVTEHDSELFCQFEYSTALFEKETIQRWASLFTTLVEHTAASPE
                     TELDDIPILTKKEERDFIESCHLFEETGYSMNQTLHYALEQQAAKTPDQAAVIFEDGAM
                     TYKELNEQANRIAWELIGRGVKSGTTIAILGRRSPEMLIGIYGILKAGGAYLPIDPDYP
                     EERISFLLKDSGTNILLLQSADLHVPAFAGEIFYLNQTNSGLKHRLSNPNTDVLPQSLA
                     YVIYTSGSTGMPKGVEVEHRSAVNFLNSLQSRYQLKQSDVIMHKTSYSFDASIWELFWW
                     PYAGASVYLLPQGGEKEPEVIAKAIEEHEITAMHFVPSMLHAFLDHIKNRSVPIKKTNG
                     LKRVFSGGEQLGAHLVSRFSELLPNVALTNSYGPTEATVEASFFDCPLHEKLERIPIGK
                     PVHHVRLYILNQKQRMLPAGCIGELYIAGAGVARGYLNRPSLTEERFLDDPFYPGERMY
                     KTGDVARWLPDGNIEFLGRTDDQVKIRGYRIEPGEIEAALRSIGGIREAAVTVRTDSGE
                     PELCAYIEGLPRNEVRAQLERRLPGYMIPAHMIEMGQWPVTPSGKLDRNALPAPGGAAD
                     AETYTAPRNVTEMKLAQLWEDVLKNGPVGIHDNFFDRGGHSLKAT"
     source          1..9758
                     /collection_date="2015"
                     /db_xref="taxon:1913579"
                     /geo_loc_name="Denmark"
                     /isolation_source="raw milk"
                     /lat_lon="56.00298848 N 9.85198974 E"
                     /mol_type="genomic DNA"
                     /organism="Bacillus sp. FMQ74"
                     /strain="FMQ74"
                     /submitter_seqid="NODE_27_length_9758_cov_132.25"
     region          1..9758
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9758
                     /SMILES="NC(C(C)C)C(=O)N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(378..443)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0021"
                     /evalue="1.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1911"
                     /protein_start="1889"
                     /score="39.6"
                     /tool="antismash"
                     /translation="FLGRTDDQVKIRGYRIEPGEIE"
     aSDomain        complement(408..1613)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21925_AMP-binding.2"
                     /evalue="9.00E-114"
                     /label="BLL41_RS21925_AMP-binding.2"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1901"
                     /protein_start="1499"
                     /score="372.2"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="LEQQAAKTPDQAAVIFEDGAMTYKELNEQANRIAWELIGRGVKSG
                     TTIAILGRRSPEMLIGIYGILKAGGAYLPIDPDYPEERISFLLKDSGTNILLLQSADLH
                     VPAFAGEIFYLNQTNSGLKHRLSNPNTDVLPQSLAYVIYTSGSTGMPKGVEVEHRSAVN
                     FLNSLQSRYQLKQSDVIMHKTSYSFDASIWELFWWPYAGASVYLLPQGGEKEPEVIAKA
                     IEEHEITAMHFVPSMLHAFLDHIKNRSVPIKKTNGLKRVFSGGEQLGAHLVSRFSELLP
                     NVALTNSYGPTEATVEASFFDCPLHEKLERIPIGKPVHHVRLYILNQKQRMLPAGCIGE
                     LYIAGAGVARGYLNRPSLTEERFLDDPFYPGERMYKTGDVARWLPDGNIEFLGRTDDQV
                     KIR"
     aSModule        complement(408..2948)
                     /domains="nrpspksdomains_BLL41_RS21925_Condensation_LCL.1"
                     /domains="nrpspksdomains_BLL41_RS21925_AMP-binding.2"
                     /incomplete
                     /locus_tags="BLL41_RS21925"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(525..614)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0020"
                     /evalue="1.10E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1862"
                     /protein_start="1832"
                     /score="56.0"
                     /tool="antismash"
                     /translation="PAGCIGELYIAGAGVARGYLNRPSLTEERF"
     CDS_motif       complement(723..752)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0019"
                     /evalue="4.40E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1796"
                     /protein_start="1786"
                     /score="6.9"
                     /tool="antismash"
                     /translation="SYGPTEATVE"
     CDS_motif       complement(1140..1199)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0018"
                     /evalue="9.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1657"
                     /protein_start="1637"
                     /score="30.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGMPKGVEVEH"
     CDS_motif       complement(1386..1424)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0017"
                     /evalue="8.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1575"
                     /protein_start="1562"
                     /score="15.8"
                     /tool="antismash"
                     /translation="GILKAGGAYLPID"
     CDS_motif       complement(1953..2072)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0016"
                     /evalue="6.70E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1386"
                     /protein_start="1346"
                     /score="66.7"
                     /tool="antismash"
                     /translation="RQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVL"
     aSDomain        complement(2112..2948)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21925_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.60E-102"
                     /label="BLL41_RS21925_Condensation_LCL.1"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1333"
                     /protein_start="1054"
                     /score="332.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="AAYPVSSAQKRIYVLQQLDDGVAYNMPAVLELEGTLDVANLSAVC
                     KELISRHEPLRTSFVSGADGEPVQRIHTEVPFELSRETDIERFIRPFDLSRAPLFRAGL
                     VKVSNEKHVLLVDMHHIISDGVSVQLLIREFTDLYAKRQLKPLRIQYKDYAVWQQLFKK
                     GDSYQKQETYWLQQFSGDLPVLELPTDKRRPAERKFSGGKVTFQMDKEITAKIKRLAHK
                     NKSTLYMALLALYSAFLSRLSAQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRP"
     CDS_motif       complement(2121..2210)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0015"
                     /evalue="2.70E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1330"
                     /protein_start="1300"
                     /score="51.8"
                     /tool="antismash"
                     /translation="DIVIGSPIAGRPHADLEAVLGMFVNTLALR"
     CDS_motif       complement(2472..2501)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0014"
                     /evalue="4.80E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1213"
                     /protein_start="1203"
                     /score="12.8"
                     /tool="antismash"
                     /translation="QYKDYAVWQQ"
     CDS_motif       complement(2532..2597)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0013"
                     /evalue="1.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1193"
                     /protein_start="1171"
                     /score="30.0"
                     /tool="antismash"
                     /translation="MHHIISDGVSVQLLIREFTDLY"
     CDS_motif       complement(2769..2882)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0012"
                     /evalue="5.60E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1114"
                     /protein_start="1076"
                     /score="47.7"
                     /tool="antismash"
                     /translation="AYNMPAVLELEGTLDVANLSAVCKELISRHEPLRTSFV"
     aSDomain        complement(2991..3197)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21925_PCP.1"
                     /evalue="4.00E-23"
                     /label="BLL41_RS21925_PCP.1"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="1040"
                     /protein_start="971"
                     /score="73.4"
                     /tool="antismash"
                     /translation="EAKLSQLFEDVLKNGSIGIQDNFFDHGGHSLKATVLMSRIAKEFH
                     VQVPLKDIFAYPTVEGLASIIRNA"
     aSModule        complement(2991..6089)
                     /complete
                     /domains="nrpspksdomains_BLL41_RS21925_Condensation_DCL.1"
                     /domains="nrpspksdomains_BLL41_RS21925_AMP-binding.1"
                     /domains="nrpspksdomains_BLL41_RS21925_PCP.1"
                     /locus_tags="BLL41_RS21925"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(3486..3551)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0011"
                     /evalue="6.70E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="875"
                     /protein_start="853"
                     /score="37.3"
                     /tool="antismash"
                     /translation="YIGRADNQVKIRGYRIEPQEIE"
     aSDomain        complement(3516..4706)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21925_AMP-binding.1"
                     /evalue="2.80E-104"
                     /label="BLL41_RS21925_AMP-binding.1"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="865"
                     /protein_start="468"
                     /score="340.9"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FETQAEKTPDAYAVIDQACSLTYKELNKSANRLARHLRMKGVARQ
                     EPVAIMIERSAAFITGVLGILKAGGAIVPIDPYYPADRIRYILNDCGCSHVVSQAHLSS
                     SLENNYIITHAEDIESKEDGSNMKSVNNADDLLYIIYTSGTTGKPKGVQFEHRNMANLL
                     KFEYVHSGIDFEADVLQFATPAFDVCYQEIFSALLKGGTLHIVPEAIKRDVPQLFTFIN
                     KHQTNIVFLPTAFIKMIFSERELANSFPYGVKHLIAAGEQLMISDLFQDVLRERGIHLH
                     NHYGPSETHVVSTYTIQPGDPIPKFPPIGRPIGCTDLYILNHQKQLQPYGVPGELYISG
                     ASVARGYVNYDKLTRDKFSSDPFRPGAIMYRTGDLARRLEDGNIEYIGRADNQVKIR"
     CDS_motif       complement(3633..3722)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0010"
                     /evalue="4.50E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="826"
                     /protein_start="796"
                     /score="38.2"
                     /tool="antismash"
                     /translation="PYGVPGELYISGASVARGYVNYDKLTRDKF"
     CDS_motif       complement(3831..3857)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0009"
                     /evalue="4.60E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="760"
                     /protein_start="751"
                     /score="4.1"
                     /tool="antismash"
                     /translation="YGPSETHVV"
     CDS_motif       complement(4239..4295)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0008"
                     /evalue="5.90E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="624"
                     /protein_start="605"
                     /score="28.0"
                     /tool="antismash"
                     /translation="YIIYTSGTTGKPKGVQFEH"
     CDS_motif       complement(4479..4520)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0007"
                     /evalue="3.00E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="544"
                     /protein_start="530"
                     /score="14.2"
                     /tool="antismash"
                     /translation="LGILKAGGAIVPID"
     CDS_motif       complement(5043..5159)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0006"
                     /evalue="1.20E-20"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="356"
                     /protein_start="317"
                     /score="66.0"
                     /tool="antismash"
                     /translation="QKDRLAAEAYSYHPLYEIQARSAVKQGLIDHILVFENYP"
     aSDomain        complement(5199..6089)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21925_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.50E-117"
                     /label="BLL41_RS21925_Condensation_DCL.1"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="304"
                     /protein_start="7"
                     /score="382.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFAIVGKVNPQYFQN
                     SINALVARHDIFRTIFISQNVSSPQQVVLRERNVIVQEKDITHLNEEKQSQFIEQLKEK
                     DRAQGFHLQKDLLMRITLIQTGEGQYTCIWTFHHIMMDGWCLSIVLKEFLHIYASYVNE
                     SAISLEPVQPYGRYIKWLMEQDKKQAVSYWDHYLSGYEQQTMLPKQKKTKGRSRQEHVT
                     FSFSKKESSRLSELAAREEVTLSTIFHTIWGILLQKYNNHDDAVFGSVISGRPAEIEGI
                     EHMVGLFINTIPVRMQ"
     CDS_motif       complement(5205..5300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0005"
                     /evalue="2.90E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="302"
                     /protein_start="270"
                     /score="64.7"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPAEIEGIEHMVGLFINTIPVR"
     CDS_motif       complement(5535..5573)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0004"
                     /evalue="2.00E-02"
                     /label="C4_DCL_171-183"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="192"
                     /protein_start="179"
                     /score="8.1"
                     /tool="antismash"
                     /translation="PYGRYIKWLMEQD"
     CDS_motif       complement(5619..5684)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0003"
                     /evalue="5.40E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="164"
                     /protein_start="142"
                     /score="34.9"
                     /tool="antismash"
                     /translation="FHHIMMDGWCLSIVLKEFLHIY"
     CDS_motif       complement(5904..6017)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0002"
                     /evalue="3.30E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="69"
                     /protein_start="31"
                     /score="45.1"
                     /tool="antismash"
                     /translation="AYVEQASFAIVGKVNPQYFQNSINALVARHDIFRTIFI"
     aSDomain        complement(6021..6110)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21925_NRPS-COM_Nterm.1"
                     /evalue="2.00E-18"
                     /label="BLL41_RS21925_NRPS-COM_Nterm.1"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="30"
                     /protein_start="0"
                     /score="57.3"
                     /tool="antismash"
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDS"
     CDS_motif       complement(6039..6080)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21925_0001"
                     /evalue="9.60E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="BLL41_RS21925"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.4"
                     /tool="antismash"
                     /translation="YPLSYMQEGMLFHS"
     CDS             complement(6136..>9754)
                     /NRPS_PKS="Domain: AMP-binding (161-554). E-value:
                     2.1e-114. Score: 374.2. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21930_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (659-727). E-value: 8.8e-25. Score:
                     78.7. Matches aSDomain: nrpspksdomains_BLL41_RS21930_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (737-1039). E-value:
                     1.3e-162. Score: 534.3. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21930_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1045-1199). E-value: 7.9e-52.
                     Score: 167.1. Matches aSDomain:
                     nrpspksdomains_BLL41_RS21930_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="BLL41_RS21930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21645"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_083428100.1"
                     /sec_met_domain="AMP-binding (E-value: 7.1e-123, bitscore:
                     397.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-16, bitscore:
                     48.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7.1e-78, bitscore:
                     248.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VQYLQEVRETALEAYEHQDYPFEELVDKLELTRDMSRNPLFDVMF
                     VLQNMDQESLDLDELCLKPAANNGHQTSKFDLTLYAQEQPRGLLTFQMEFSTDLYKKKT
                     IEKWLQYFINMLLSIIQDNKAVLGTINILNEDETHYLIHELNRTKMDYPRNETISRLFE
                     VQAEQTPNAVAIVSDTQVLTYEDLNSWANQIASVLQSKGVGPDSVVALLTGRTPELIAG
                     ILGILKAGGAYLPIASDLPVERIAYMLSDSRAALLLQSEKTEKQLLDIECEQIIIEDIQ
                     KQGEAKNVESSAGPHSLAYIIYTSGSTGKPKGVMIEQRSVIRLVKNSNYITFTPADRLL
                     MTSSIGFDVGSFEFFGPLLNGAALHLSDQQTFLDSQQLKRYIEHQGITTMWLTSSLFNH
                     LTEQLETTFSQLNHLIIGGEALSASHVNRIRNACPEVSIWNGYGPTENTTFSTCFHIQK
                     TYEVSIPIGRPVGNSTAYILNQWGMLQPVGAVGELCVGGDGVARGYLRRPDLTKKKFVQ
                     NPFVPGDRLYRTGDLARWLPDGTIEYVGRIDDQVKVRGYRVELGEIESALRQIDGVKEA
                     AVLARTGQSGSKELFGYISVKAGTNAEQVRSLLERSLPNYMIPAYFIEMENFPLTSNGK
                     LNRSALPEPDFASKQTYIPPRNELEEQLALIWQEVLGIKRIGIEDSFFELGGDSIKALQ
                     VSARLGRHGLSLQVSDLFRHPKIKDLSPFIRKTERVIEQGPVQGDVPWTPIQKWFLSQD
                     IEEQHHFNQSVMLFHSDRLSEDALRASLKKLAEHHDALRMVYRKDDGRWIQINQSIHES
                     QLYRLRTSDLSQSESGWETQIKLEVADLQQSINLQEGPLLHAAWFKTLTGDYLFLAIHH
                     LVVDGVAWRILLEDLSAGYQQASSGQAIQLPPKTDSYQEYAKRIQEYAQSSKLIREEAY
                     WRSAEEQQTAELPYEMPFHENTDCSKRDSVSFSLSEADTDVLLQNVNHAYGTDTQDILL
                     TAASLAICEWAGGSKLRIAMEGHGREHIIPELDISRTVGWFTSMYPALISFENQRDELG
                     TAVKTVKDTLGRIPNKGIGYGILKYLTHPENKSIAFSKTPEISFNYLGQFNDIERQGSF
                     RPSSLGSGKDITHTWKREQIIEMSAMAADKKLHFNLSYPPARFHRNTMDQLINRIECFL
                     LDIMKHCAGKQKSEKTLSDFSSQSLTAEDLDSISSLVEEL"
     gene            complement(6136..>9758)
                     /locus_tag="BLL41_RS21930"
                     /old_locus_tag="BLL41_21645"
     aSDomain        complement(6157..6618)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21930_TIGR01720.1"
                     /evalue="7.90E-52"
                     /label="BLL41_RS21930_TIGR01720.1"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="1199"
                     /protein_start="1045"
                     /score="167.1"
                     /tool="antismash"
                     /translation="ELGTAVKTVKDTLGRIPNKGIGYGILKYLTHPENKSIAFSKTPEI
                     SFNYLGQFNDIERQGSFRPSSLGSGKDITHTWKREQIIEMSAMAADKKLHFNLSYPPAR
                     FHRNTMDQLINRIECFLLDIMKHCAGKQKSEKTLSDFSSQSLTAEDLDSI"
     aSModule        complement(6157..9270)
                     /complete
                     /domains="nrpspksdomains_BLL41_RS21930_AMP-binding.1"
                     /domains="nrpspksdomains_BLL41_RS21930_PCP.1"
                     /domains="nrpspksdomains_BLL41_RS21930_Epimerization.1"
                     /domains="nrpspksdomains_BLL41_RS21930_TIGR01720.1"
                     /locus_tags="BLL41_RS21930"
                     /monomer_pairings="Val -> D-Val"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(6463..6492)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0010"
                     /evalue="3.80E-03"
                     /label="NRPS-E7"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="1097"
                     /protein_start="1087"
                     /score="9.8"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     CDS_motif       complement(6532..6594)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0009"
                     /evalue="3.70E-10"
                     /label="NRPS-E6"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="1074"
                     /protein_start="1053"
                     /score="31.9"
                     /tool="antismash"
                     /translation="VKDTLGRIPNKGIGYGILKYL"
     aSDomain        complement(6637..7542)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21930_Epimerization.1"
                     /evalue="1.30E-162"
                     /label="BLL41_RS21930_Epimerization.1"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="1039"
                     /protein_start="737"
                     /score="534.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GDVPWTPIQKWFLSQDIEEQHHFNQSVMLFHSDRLSEDALRASLK
                     KLAEHHDALRMVYRKDDGRWIQINQSIHESQLYRLRTSDLSQSESGWETQIKLEVADLQ
                     QSINLQEGPLLHAAWFKTLTGDYLFLAIHHLVVDGVAWRILLEDLSAGYQQASSGQAIQ
                     LPPKTDSYQEYAKRIQEYAQSSKLIREEAYWRSAEEQQTAELPYEMPFHENTDCSKRDS
                     VSFSLSEADTDVLLQNVNHAYGTDTQDILLTAASLAICEWAGGSKLRIAMEGHGREHII
                     PELDISRTVGWFTSMYPALIS"
     CDS_motif       complement(6643..6690)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0008"
                     /evalue="5.70E-09"
                     /label="NRPS-E5"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="1037"
                     /protein_start="1021"
                     /score="27.9"
                     /tool="antismash"
                     /translation="DISRTVGWFTSMYPAL"
     CDS_motif       complement(7096..7161)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0007"
                     /evalue="3.10E-11"
                     /label="NRPS-E2"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="886"
                     /protein_start="864"
                     /score="35.4"
                     /tool="antismash"
                     /translation="LFLAIHHLVVDGVAWRILLEDL"
     CDS_motif       complement(7366..7443)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0006"
                     /evalue="4.00E-05"
                     /label="C2_LCL_024-062"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="796"
                     /protein_start="770"
                     /score="16.0"
                     /tool="antismash"
                     /translation="RLSEDALRASLKKLAEHHDALRMVYR"
     aSDomain        complement(7573..7776)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21930_PCP.1"
                     /evalue="8.80E-25"
                     /label="BLL41_RS21930_PCP.1"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="727"
                     /protein_start="659"
                     /score="78.7"
                     /tool="antismash"
                     /translation="EEQLALIWQEVLGIKRIGIEDSFFELGGDSIKALQVSARLGRHGL
                     SLQVSDLFRHPKIKDLSPFIRKT"
     CDS_motif       complement(8062..8127)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0005"
                     /evalue="1.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="564"
                     /protein_start="542"
                     /score="39.1"
                     /tool="antismash"
                     /translation="YVGRIDDQVKVRGYRVELGEIE"
     aSDomain        complement(8092..9270)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLL41_RS21930_AMP-binding.1"
                     /evalue="2.10E-114"
                     /label="BLL41_RS21930_AMP-binding.1"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="554"
                     /protein_start="161"
                     /score="374.2"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEVQAEQTPNAVAIVSDTQVLTYEDLNSWANQIASVLQSKGVGPD
                     SVVALLTGRTPELIAGILGILKAGGAYLPIASDLPVERIAYMLSDSRAALLLQSEKTEK
                     QLLDIECEQIIIEDIQKQGEAKNVESSAGPHSLAYIIYTSGSTGKPKGVMIEQRSVIRL
                     VKNSNYITFTPADRLLMTSSIGFDVGSFEFFGPLLNGAALHLSDQQTFLDSQQLKRYIE
                     HQGITTMWLTSSLFNHLTEQLETTFSQLNHLIIGGEALSASHVNRIRNACPEVSIWNGY
                     GPTENTTFSTCFHIQKTYEVSIPIGRPVGNSTAYILNQWGMLQPVGAVGELCVGGDGVA
                     RGYLRRPDLTKKKFVQNPFVPGDRLYRTGDLARWLPDGTIEYVGRIDDQVKVR"
     CDS_motif       complement(8209..8298)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0004"
                     /evalue="2.70E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="515"
                     /protein_start="485"
                     /score="54.8"
                     /tool="antismash"
                     /translation="PVGAVGELCVGGDGVARGYLRRPDLTKKKF"
     CDS_motif       complement(8803..8859)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0003"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="317"
                     /protein_start="298"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGKPKGVMIE"
     CDS_motif       complement(9046..9084)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0002"
                     /evalue="1.60E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="236"
                     /protein_start="223"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPI"
     CDS_motif       complement(9613..9732)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLL41_RS21930_0001"
                     /evalue="2.90E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BLL41_RS21930"
                     /protein_end="47"
                     /protein_start="7"
                     /score="74.4"
                     /tool="antismash"
                     /translation="RETALEAYEHQDYPFEELVDKLELTRDMSRNPLFDVMFVL"
ORIGIN
        1 gttgctttta aggaatgtcc cccgcggtca aaaaagttgt cgtgaatccc gacagggccg
       61 tttttcagga cgtcctccca cagctgggca agcttcatct cggtcacatt tcttggcgct
      121 gtgtaggtct ccgcatctgc cgctccgcca ggagctggca gggcgtttcg gtccagcttt
      181 ccgcttggcg taaccggcca ctgccccatc tcgatcatat gggctgggat catgtagccc
      241 ggcagccgtc tctcaagctg cgcccgcact tcgtttctcg ggagcccttc tatatatgcg
      301 cataattccg gctcgccgct gtctgtccgt acggtaacgg cagcttccct tatgcctcct
      361 atgcttctaa gcgccgcttc aatttctccg ggctcaatcc ggtagccgcg gattttcacc
      421 tgatcatccg tgcggccgag gaattctata ttcccgtcag ggagccagcg ggctacatcc
      481 cctgttttgt acatgcgttc acccgggtaa aacggatcgt caagaaaacg ctcctccgtt
      541 aagctcggcc gatttaaata gcctctggcc acacccgctc cggcaatgta cagttcgccg
      601 atacatccgg caggcaacat ccgctgcttc tgattgagaa tatacaatct gacatgatga
      661 acaggtttgc caatcggaat tcgttcaagt ttttcatgca gcggacaatc aaagaatgaa
      721 gcttctacag ttgcctccgt tggtccgtag gaattggtta atgcaacatt tggaagcagt
      781 tcgctaaatc gggagacgag atgggctccc agctgctctc ccccggaaaa cacccgcttg
      841 agtccgtttg tcttcttaat cggtacagag cgatttttta tatggtctaa aaaggcatgg
      901 agcattgaag gcacaaaatg catagctgtg atctcgtgtt cttctatggc ctttgcgatc
      961 acttcaggtt ctttttcgcc tccctgagga agcagataaa cagaagcacc ggcataaggc
     1021 caccaaaaca gctcccatat tgatgcgtcg aatgaataag atgttttatg cataatcaca
     1081 tctgattgct tcagttgata acgggactga agggaattca aaaaattcac cgctgatcga
     1141 tgttcaactt caacaccctt aggcatcccg gtagagccgg atgtatagat gacataggct
     1201 aaagattgcg ggagaacgtc cgtattcgga ttggacaaac gatgtttcag cccgctattt
     1261 gtctgattga gataaaaaat ctctcctgca aatgccggaa catgaaggtc ggcagactga
     1321 agcaggagaa tgtttgtacc gctgtctttg agcagaaagc tgattcgttc ttccgggtaa
     1381 tccggatcaa tgggcagata agcgccgcca gctttaagaa tgccataaat gccaatcagc
     1441 atttcaggtg aacgcctgcc taaaatggca atagtcgttc cagattttac tccccgtccg
     1501 atcagctccc aggcgatacg gttagcctgt tcgttcaatt ctttataggt catcgctcca
     1561 tcttcaaaga taacggccgc ctgatcaggc gtttttgctg cctgctgttc gagtgcatag
     1621 tggagagttt gattcattga atagcccgtc tcctcaaaca gatggcatga ttcaatgaaa
     1681 tctctctctt ctttttttgt taagattgga atatcgtcta attctgtttc aggactggca
     1741 gctgtatgtt ccacaagcgt ggtaaaaagg ctggcccacc tttggatggt ttctttctca
     1801 aacaaagctg tactgtattc aaactgacag aaaagctcag aatcatgctc tgtaacgtat
     1861 agggaaatat caaattttga tattgggtgc tccaagtcgt tccactgaag ctgtacatca
     1921 ttcattttta gcttttgccg ttccatattc tgaaggacta gcgttgtgtc aaacaaagga
     1981 ttccggctca tttcccgctg cacaccgagt ttgtccacaa gctcttcaaa cgggtaatcc
     2041 tgatgctcat acgcttctag tgccgtctgt ctgacttctt tcagaaattc ttcaaatgtc
     2101 ttattaccgg ccgggcgtgt gcggagagcc aatgtattga cgaacatccc gagaacagct
     2161 tcaagatcgg catggggccg tccggcgata ggtgaaccaa ttacgatatc atcctgagcg
     2221 cttaaccgcg acaaaaaggc tgaatacaaa gcgagaagcg ccatgtaaag tgtgcttttg
     2281 tttttatggg cgagtcgctt tatcttggcc gtgatctctt tatccatttg aaaggttact
     2341 tttccgcctg agaatttccg ttctgccgga cggcgtttat ctgtcggaag ctctagtaca
     2401 gggaggtctc cgctaaactg ctgcagccag taagtctcct gcttttgata cgagtctccc
     2461 tttttaaaaa gctgctgcca gacggcgtaa tctttatact ggatccgcag cggcttaagc
     2521 tgccgttttg cataaagatc ggtgaattct cggattaata gctgaacgga tacgccatct
     2581 gaaataatat ggtgcatatc caccagcaat acatgcttct cgttcgacac ttttacaaga
     2641 cccgccctaa ataacggcgc ccggctgaga tcaaagggac gaataaatct ctcgatatcg
     2701 gtctctctgc ttaattcaaa cggtacctcg gtgtgaatac gctgaacagg ctcgccatca
     2761 gccccggata caaaggaagt tcgaagaggc tcatgccgac tgatcagttc tttacataca
     2821 gcagacaggt tagcgacatc caatgtccct tccagttcta agaccgcagg catattgtag
     2881 gcgacaccat catcaagctg ctgaagcaca tagatccgtt tttgagcgga agatacagga
     2941 tatgccgccc tttcttctgc cggttcaatt gctgcatatg gattttcttc tgcatttcga
     3001 atgattgaag ctaacccttc cactgtcgga taggcaaaaa tgtccttaag cggaacttgc
     3061 acatgaaatt cctttgcaat ccgtgacatc agcactgttg cttttagaga gtggccacca
     3121 tggtcaaaaa agttgtcttg gatgcctata gaaccattct tcagcacatc ttcaaatagt
     3181 tgtgaaagct ttgcctctaa cagattacga ggggcagtat atggatttcc gctgatcgaa
     3241 gcatctggct ctggaagtgc tgattgatct actttgccat ttggggtaag aggtatgctg
     3301 tcaacccata cccatttcgc cggaatcatg tattctggta gctcactagc catatagctg
     3361 cgtaaatcta ttgcattcag cttttgtaca ctgcaatagt aagcgcataa ttcatgttca
     3421 ccgttttgat cttgcctgat gagaatggca gcctctttca tgtccggatg attcattaac
     3481 gtgacttcga tttcttgcgg ctcgattcga tagcccctga ttttcacttg gttgtctgct
     3541 cgtccgatat attcgatgtt gccgtcctca agccgtctgg ctaaatcacc tgtacggtac
     3601 atgatcgcac caggcctgaa tgggtcggat gaaaacttgt cgcgtgtcag tttatcgtaa
     3661 ttgacatatc ctctggcaac acttgcgccg gaaatataga gttcacctgg tacaccgtat
     3721 ggctgtagct gtttttgatg attcaaaata tatagatcag tacagcctat cggtctaccg
     3781 attggcggaa acttcggaat agggtctccc ggttgaatgg tatacgtaga taccacatga
     3841 gtttctgaag gtccgtaatg attgtgcaga tgtattccgc gttcacgtaa cacgtcttgg
     3901 aaaaggtctg aaatcattaa ttgttccccg gctgcaatca agtgtttgac gccataagga
     3961 aacgagttcg caagttctcg ctcgctgaaa atcattttga taaaagcagt tgggagaaac
     4021 acaatattcg tctgatgctt gtttataaat gtaaacagct gaggcacatc tctttttatg
     4081 gcttctggca cgatgtggag tgtgccgcct ttcagaagcg cagaaaatat ttcttgatag
     4141 cagacgtcaa aggcaggtgt cgcaaattgc agaacatctg cttcgaagtc aatgccagag
     4201 tgtacatatt cgaatttcag caagttagcc atgtttcgat gctcaaattg aacacccttt
     4261 ggtttacctg ttgtacctga cgtataaatg atatacagca gatcgtcagc attattgact
     4321 gacttcatgt tactgccgtc ttctttgctt tcgatatctt ctgcatgggt gataatgtaa
     4381 ttgttttcta acgatgagga aagatgcgct tgtgaaacaa catgcgaaca gccgcagtca
     4441 tttagaatat aacggattct gtcagctgga taatacggat caatcggaac aatggcgccg
     4501 cctgccttta agattccaag aacacctgtg ataaacgccg ctgagcgttc tatcataatc
     4561 gcaacgggct cctgtctcgc aacacctttc attcgtaaat gccgcgccaa tctattagcc
     4621 gatttattca gttctttgta tgttaacgag caggcttgat caattacagc ataagcatcc
     4681 ggcgtctttt ccgcttgtgt ttcaaacatg gcatggatgg tttcgtaaac tggtgttcga
     4741 tctgcgattt ctccaatcag ctgccgcttt tcagtatctg tcgtaatctc tatttcacca
     4801 ggtggaagat tcggattgtg taagatttgc gatacgacat tcagcagatg ctccttgaca
     4861 gaaagcacaa aagaccgatc atgctgctca gcatcatagc acattttcat atggatttct
     4921 tctcctggtg ctaccataag ataaaagctg tagttggttt catctgccac agtgaagtta
     4981 tgaatctgaa aaagatctga agcatgttct tgtttgttga gcatttgaat ctcttgctgc
     5041 accggatagt tttcaaatac gagaatatgg tcaatcagcc cttgcttaac cgctgaacgg
     5101 gcttgaattt catataaagg gtggtagctg tatgcttcag cggcaagacg gtctttttgc
     5161 atatctttta tgagctgtag aaatggtgtt tttgccccct gcatccgaac gggtatcgtg
     5221 ttaatgaaaa ggcctaccat atgttcaatg ccttcgattt ctgcgggtct tccagaaatg
     5281 acagaaccga atacggcgtc atcgtgatta ttatattttt gaagcagaat gccccaaatg
     5341 gtatggaaaa tcgtactgag tgtcacttct tctcttgccg caagctccga aagcctgctg
     5401 ctttctttct tagagaatga aaacgtaacg tgttcctgcc tgcttctccc ttttgttttt
     5461 ttctgctttg ggagcatggt ttgctgttca tagccggaaa ggtaatgatc ccaatatgaa
     5521 actgcctgct ttttgtcttg ttccatcagc catttgatat accttccgta tggctgaacc
     5581 ggctctagtg atatagcgga ttcatttaca taagatgcat aaatatggag aaactccttt
     5641 aacacgatgc ttaggcacca gccgtccatc atgatgtgat gaaaggtcca aatacaagtg
     5701 tattggccct ctcctgtttg aatcagagtg atgcgcatga gcagatcttt ttgcaagtga
     5761 aacccttgag cgcggtcttt ttctttcaat tgttcgataa actgtgattg cttctcttcg
     5821 tttaaatgcg taatgtcttt ttcctgcacg atgacattcc gttctctcag gacaacctgc
     5881 tggggagagg aaacattttg actgataaaa attgtcctga aaatatcgtg tcttgctaca
     5941 agagcattga tgctgttttg aaaatactgc gggttgactt ttcctactat tgcaaaagaa
     6001 gcttgctcaa catatgcctg tgaatctttt tgcaggaggg aatggaaaag catcccttcc
     6061 tgcatgtaag acagcggata aatatcctga attgaattcg cttttgtcat gttcatgtct
     6121 ccctgtcacg attttctaca attcttccac caagctggat atactgtcca aatcctctgc
     6181 cgttaatgat tgactgctaa aatcgctcag tgtcttttct gatttttgtt taccggcgca
     6241 gtgtttcata atgtccagca aaaagcattc gatcctgttg ataagctggt ccattgtgtt
     6301 tctatgaaag cgggctggcg gatagctaag attgaaatga agctttttgt ctgctgccat
     6361 tgcactcatc tctatgattt gctcacgctt ccatgtatga gtgatatcct ttccgcttcc
     6421 aagacttgat gggcggaagc tgccctgtct ttcgatgtcg ttgaactggc ccaaatagtt
     6481 aaagctgatt tcaggtgttt ttgaaaacgc aatgcttttg ttttcagggt gtgtgaggta
     6541 tttcagtata ccgtaaccta tccctttgtt tggtatccgg cccagtgtat ctttaacggt
     6601 ttttactgcg gttcccagct catccctttg attttcaaag cttatgaggg ccgggtacat
     6661 ggatgtaaac cagccgaccg tccggctgat atccaactcc ggtataatgt gctctcttcc
     6721 gtgtccttcc atagcgattc ggagcttgct tccgcctgcc cattcacaaa tcgctaacga
     6781 tgccgccgtt aacagaatat cttgtgtatc tgtaccatag gcgtgattga cgttttgcag
     6841 caaaacgtca gtgtctgctt cagagagtga aaagcttacc gagtccctct tgctgcagtc
     6901 ggtgttctca tgaaacggca tttcatatgg cagttccgca gtttgctgtt cttctgcact
     6961 tcgccaatac gcctcttcac gaatcagctt gctgctttgg gcatattcct gaatccgttt
     7021 ggcgtattcc tggtaggaat ccgttttagg cggaagctga atcgcctggc ctgaagacgc
     7081 ttgttggtac cctgctgaca aatcctccag caaaatccgc catgccacac cgtcaacgac
     7141 aagatgatgg attgcaagga acaagtaatc tccggtcagt gttttaaacc acgcggcatg
     7201 taacaatggt ccttcctgca aattgatgct ttgctgaaga tccgcaactt ctagtttgat
     7261 ttgtgtttcc caaccgcttt cggactgtga aagatcagat gttcttaacc tgtatagctg
     7321 tgattcatgg atgctttgat tgatttgaat ccatcgtccg tcatctttac gataaaccat
     7381 ccgaagcgca tcatgatgct ccgccagctt ttttaaggat gctctaagag cgtcttcaga
     7441 caaacggtca gaatggaaaa gcatgacaga ctgattgaaa tggtgttgtt cttctatatc
     7501 ttgtgagaga aaccatttct gaataggcgt ccatggaaca tctccttgaa caggtccttg
     7561 ttctatgact ctctctgttt tacgaataaa tggtgataag tcttttatct ttggatgacg
     7621 gaataaatca cttacctgca agcttaaccc gtggcgcccg agacgtgcag aaacttgaag
     7681 cgccttgatt gaatcgccgc ccaattcaaa gaatgaatcc tctattccga tcctcttgat
     7741 gcctagaacc tcctgccaga tcaatgccag ctgctcttca agttcgttgc gtggcgggat
     7801 gtatgtttgt ttagatgcga aatctggttc aggcagtgca ctccggttca atttgccgtt
     7861 cgatgtgagc ggaaaattct ccatttcaat gaaatacgcc ggaatcatat aatttggcag
     7921 tgaacgctcg agaagagaac gcacttgttc agcattcgtg cccgctttta cgctgatgta
     7981 gccaaacagc tctttgctcc ctgactgtcc tgtacgtgcc aacaccgccg cttctttgac
     8041 tccgtctatt tggcgaagtg ctgattcgat ctcacccagc tctacccgat agcctctgac
     8101 ttttacttga tcatcaatac ggccaacgta ctcaattgtg ccatcgggaa gccagcgcgc
     8161 cagatcacct gtgcggtaca gtcgatcgcc gggtacaaat gggttttgga caaatttctt
     8221 tttcgttaaa tctggacggc gtaaatatcc tctggcaacg ccatctccgc cgacacaaag
     8281 ttcgccaaca gctccaactg gctgcagcat tccccattga tttaagatat aagcggtgga
     8341 attgccaacc ggccgcccga tcggaatgga gacttcatat gttttttgaa tgtggaagca
     8401 tgtcgaaaaa gttgtatttt cggttggccc atatccgttc caaatagata cttcagggca
     8461 ggcattcctg attcgattga cgtggcttgc cgacaaagct tctccgccta tgatcagatg
     8521 gttaagctgc gaaaatgttg tctcgagttg ctctgtcaaa tgattgaata gagacgaggt
     8581 cagccacatc gtagtgattc cttgatgttc tatgtatctc ttaagctgtt gggaatcgag
     8641 aaatgtttgt tgatcggaca gatgcaaagc ggcaccattt aacaggggac caaaaaattc
     8701 aaatgatcca acgtcaaaac cgattgatga tgtcatcaga aggcggtcag ctggtgtaaa
     8761 tgtaatataa ttgctgtttt tcacaagacg aatgacactt ctctgttcaa tcatgacacc
     8821 ctttggttta ccggttgatc cagaggtata aatgatataa gcaagtgaat ggggccctgc
     8881 tgaagattca acattcttcg cctctccttg cttttgaata tcttcgatta tgatttgttc
     8941 acattcaatg tctaatagct gtttttctgt tttttcagat tgaaggagta atgctgccct
     9001 gctgtcagac agcatataag ctattcgttc aacaggcagg tcagaagcga taggcagata
     9061 ggctcccccg gctttcagaa tgcccagtat acctgctatg agttctggcg ttctgcctgt
     9121 cagcaaggct acaacactat caggccctac gcccttactc tgtagaactg aagcaatttg
     9181 atttgcccag ctgttcaggt cctcgtaagt taacacctgg gtatcgctga caatcgctac
     9241 agcattaggt gtttgttcag cctgcacctc aaagagcctg ctgattgttt catttcttgg
     9301 ataatccatt tttgtacgat tgagctcatg gataaggtaa tgggtctcat cttcattcag
     9361 gatatttatt gttccgagaa cggctttgtt gtcttggatg atggagagta acatattgat
     9421 gaagtattga agccactttt ctatcgtttt tttcttatat aagtcagtac taaactccat
     9481 ttggaacgtc agcaaaccgc gcggctgttc ctgagcatat agggttaaat caaatttgga
     9541 cgtttgatgc ccattgtttg ctgcaggttt gagacacagt tcatccaggt ccaacgattc
     9601 ttgatccatg ttttgaagaa caaacattac gtcaaacagc gggtttctgc tcatatccct
     9661 tgtcaattca agcttatcga caagctcttc gaacggataa tcctgatgct cataagcttc
     9721 caatgcagtc tcgcgcactt cctgcaaata ctgtacaa
//
