LOCUS       NZ_MOEO01000026        11057 bp    DNA     linear   CON 22-AUG-2024
DEFINITION  Bacillus sp. FMQ74 NODE_26_length_11479_cov_54.1592, whole genome
            shotgun sequence.
ACCESSION   NZ_MOEO01000026
VERSION     NZ_MOEO01000026.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. FMQ74
  ORGANISM  Bacillus sp. FMQ74
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Okshevsky,M., Marshall,I., Schreiber,L. and Meyer,R.L.
  TITLE     Draft Genome Sequence of Bacillus sp. FMQ74, a Dairy-contaminating
            Strain Isolated from Raw Milk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Okshevsky,M., Marshall,I., Schreiber,L. and Meyer,R.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) Bioscience, Aarhus University, Ny Munkegade
            116, Aarhus 8000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2016
            Assembly Method                   :: SPAdes v. 3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001875785.1-RS_2024_08_22
            Annotation Date                   :: 08/22/2024 02:46:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,310
            Genes (coding)                    :: 4,147
            CDSs (with protein)               :: 4,147
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1 (5S, 23S)
            partial rRNAs                     :: 2, 12, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 105 of 163
            Pseudo Genes (incomplete)         :: 96 of 163
            Pseudo Genes (internal stop)      :: 20 of 163
            Pseudo Genes (multiple problems)  :: 53 of 163
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:13:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 422
            Orig. end                         :: 11479
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOEO01000026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11057
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="glycocin"
                     /region_number="1"
                     /rules="(glycocin or sublancin)"
                     /tool="antismash"
     cand_cluster    1..11057
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(glycocin or sublancin)"
                     /kind="single"
                     /product="glycocin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11057
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:10171](+)"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10171
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
     misc_feature    214..228
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 19.86"
                     /tool="antismash"
     gene            complement(654..1544)
                     /gene="yokF"
                     /gene_synonym="nukF"
                     /locus_tag="BLL41_RS21850"
                     /old_locus_tag="BLL41_21565"
     CDS             complement(654..1544)
                     /codon_start=1
                     /gene="yokF"
                     /gene_synonym="nukF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390044.1"
                     /locus_tag="BLL41_RS21850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21565"
                     /product="endonuclease YokF"
                     /protein_id="WP_071579403.1"
                     /transl_table=11
                     /translation="MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKT
                     EQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDTIKVSYNGNVDTVRYLLIDTPETKK
                     PNSCVQPYGEDASKRNKELVNSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETLLKE
                     GLARVAYVYEPNTKYIDRFKKDEQEAKSEKLSIWSKNGYVTDRGFDGCVKEKTTAVKKA
                     TTSKPAAKQPTTPKASSETSTTTEKEASSETTGETETFKNCTELRKKYPNGVPSSHPAY
                     QSKMDRDHDNYACER"
     misc_feature    1561..1576
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.41"
                     /tool="antismash"
     misc_feature    complement(1562..1575)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.43"
                     /tool="antismash"
     gene            1726..1980
                     /locus_tag="BLL41_RS21855"
                     /old_locus_tag="BLL41_21570"
     CDS             1726..1980
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019712867.1"
                     /locus_tag="BLL41_RS21855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21570"
                     /product="hypothetical protein"
                     /protein_id="WP_071579404.1"
                     /transl_table=11
                     /translation="MLGIKVEKSNENLIINWQLSKIEIPIKEINDVFLDPNYGGEEKNA
                     VRIGFPYGSTDRVVIKTANNTYILFTTNAGSIMSKIVNS"
     gene            2046..2600
                     /locus_tag="BLL41_RS21860"
                     /old_locus_tag="BLL41_21575"
     CDS             2046..2600
                     /GO_process="GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389782.3"
                     /locus_tag="BLL41_RS21860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21575"
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_071579405.1"
                     /transl_table=11
                     /translation="MTNFVHKTVNGLKSLLDEKGAIKLFCSEGNIMEFLVTFSEDKATL
                     NDIQSFEAKHQLTLPKDYQKFMTLHNGAKIFQILSDGENIGGGLYLFSLEEIEEELKYE
                     DLFEGINGIPIGHLLEGCHLMIDKDKLDKGDPNYLFILQLGEYTPLNLNFEIFLDRYIE
                     SNGATFWEWRYYTAENYYRTR"
     gene            complement(2669..3061)
                     /locus_tag="BLL41_RS21865"
                     /old_locus_tag="BLL41_21580"
     CDS             complement(2669..3061)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLL41_RS21865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BLL41_21580"
                     /product="hypothetical protein"
                     /protein_id="WP_071579406.1"
                     /transl_table=11
                     /translation="MKLNLPQETINEILDLLKKRNAAEYKTEALSKYYFTYREIEKGVN
                     ILDQKLKVLEIESQFTKELKIQGKSMGFKLGAIMRDKDTAPSIEQGKLLYIYFFTNHKY
                     DLSIPEDLINEEHHHEVELRGFVIPD"
     gene            3292..5043
                     /locus_tag="BLL41_RS21870"
                     /old_locus_tag="BLL41_21585"
     CDS             3292..5043
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017696905.1"
                     /locus_tag="BLL41_RS21870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21585"
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="WP_071579407.1"
                     /transl_table=11
                     /translation="MKVFEADSLLSEADKRAKEYKELRSQMVKLKKTFKAVADLDDSEF
                     SGKGADNIKAFYHDHVGVTDQWIDLIDMKIAFLSSISAKLEDAKMSDAYIEESFLEHEL
                     ANAYTKSKSIMSEQKKAMKDILNDINDILPLEIFSTEDFKDKLSSADDKREKTIDKLNK
                     LDEDLKTEYAETEPNEQFIQQDFKKLQESTGKGKNATPIHYNAKAYRESDIHKKKGDIE
                     KHSEAYLSVKKEEEKEREIKELKKKLNDDVSDPDEYLEIAKKVGYENLEPAQAQLAVQI
                     EQAKQLEGAGEITWDIVKGVGVGLYDVGKDTVTGIWDFITDPGETLSALGNAVIHPVKT
                     YDAISAAIEESYQKDMVNGDAYSRSRWVTYAVGSVAVAVFGTKGAGAINKADAAGKVIN
                     KASQAGKKIKDVKIPDLLPYNPKYDLAMAGGVPYNVVNGESLKNRLLQLAASANPRSVL
                     PRTRGKWIGKPGDGQWYSDIPEVNAATKGEPITFKDGRPDFTPWTVANIKFKPGELNGT
                     KKDFRLVYKKIKEIKGFKSQAQAKKWLSQQGLTPHHLDSTTIQLVPSVINNKIPHIGGA
                     SDLRGGY"
     gene            5045..5641
                     /locus_tag="BLL41_RS21875"
                     /old_locus_tag="BLL41_21590"
     CDS             5045..5641
                     /GO_process="GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407917.1"
                     /locus_tag="BLL41_RS21875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21590"
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_071579408.1"
                     /transl_table=11
                     /translation="MSFTKIEQKLKEFTVDTETDPCINTNEKLKEIEMNIGNQLPSDYK
                     DFLKEYGGCYLESKKTTDEIEYDVCYKPIEKDPWMGKDDDTQLLEGFYGLANDHNSLLK
                     AIDTYSDRFPRNIIPIASSAGGNEICMDIDNEKILFWDHELSQPDKDFFLIANSFEEFI
                     LSLVDEPIETDDNVSDVEIEILDNDFGKLLFGKDN"
     gene            complement(5976..6092)
                     /gene="bsrG"
                     /locus_tag="BLL41_RS22765"
                     /old_locus_tag="BLL41_21595"
     CDS             complement(5976..6092)
                     /codon_start=1
                     /gene="bsrG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009513970.1"
                     /locus_tag="BLL41_RS22765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21595"
                     /product="type I toxin-antitoxin system toxin BsrG"
                     /protein_id="WP_009967548.1"
                     /transl_table=11
                     /translation="MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK"
     gene            6325..6792
                     /locus_tag="BLL41_RS21885"
                     /old_locus_tag="BLL41_21600"
     CDS             6325..6792
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390037.1"
                     /locus_tag="BLL41_RS21885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21600"
                     /product="DUF4879 domain-containing protein"
                     /protein_id="WP_071579409.1"
                     /transl_table=11
                     /translation="MKKRITYVLLALLAVIVFAFTDSSKAKAAEATPLYYLQITGITSD
                     GNDFAWDYINFNQTKAPSVLKGNKLHVRARFMGYTKLTVITGKDGKNLLYDGTARMYKS
                     DAILGQNKVVIGWDKYFEIPMDALQDNSIQIKALSSGTTFVYSQKIDFERE"
     gene            6798..7157
                     /locus_tag="BLL41_RS21890"
                     /old_locus_tag="BLL41_21605"
     CDS             6798..7157
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390036.1"
                     /locus_tag="BLL41_RS21890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21605"
                     /product="hypothetical protein"
                     /protein_id="WP_071579410.1"
                     /transl_table=11
                     /translation="MISTSNYDVTKDPKFLAFCSLATEGYGGNNSAPSRSIGFSEFCEK
                     LEKNEVVKPENKKSSLELNKSLDYWKSRFNPGGNLSALDSQVLDIIKKAYNLGMVNKNN
                     MLLRNEAINAYKNSI"
     gene            complement(7199..7534)
                     /locus_tag="BLL41_RS21895"
                     /old_locus_tag="BLL41_21610"
     CDS             complement(7199..7534)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389777.1"
                     /locus_tag="BLL41_RS21895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21610"
                     /product="hypothetical protein"
                     /protein_id="WP_071579411.1"
                     /transl_table=11
                     /translation="MKKRLIGFLILVPVLIISGITLIESNKKAPEEVLESAWDEFGLFS
                     FQIGITDPAITIGMDKTKSETKLREYLEHNLSREAKEKYKIYIFKDDIEKLEKEHREYL
                     KANNSNK"
     misc_feature    complement(7631..7644)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 23.81"
                     /tool="antismash"
     misc_feature    complement(7661..7674)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 23.31"
                     /tool="antismash"
     gene            7708..8040
                     /locus_tag="BLL41_RS21900"
                     /old_locus_tag="BLL41_21615"
     CDS             7708..8040
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390034.1"
                     /locus_tag="BLL41_RS21900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21615"
                     /product="YolD-like family protein"
                     /protein_id="WP_071579412.1"
                     /transl_table=11
                     /translation="MLRDRGTIKWTSMMLPEHLTQLKQDLIDVSKIEKPSLDDQQIEEM
                     DLLVSEALEFNKELKFKLFNNGFVENVTGRVHYINFEQHRLHVKDQNDNTIYINMNNII
                     GVTYND"
     gene            8033..9283
                     /locus_tag="BLL41_RS21905"
                     /old_locus_tag="BLL41_21620"
     CDS             8033..9283
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390033.2"
                     /locus_tag="BLL41_RS21905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21620"
                     /product="UV damage repair protein UvrX"
                     /protein_id="WP_071579413.1"
                     /transl_table=11
                     /translation="MIDYSQFPRKNILCVDMKSFYASVSAVTMGLNPLTCYLAVVGNTE
                     RQGSVVLAASPALKKDFGIKTGSRLFEIPEDPRIHIVNPQMKLFIRVSTEITKLFYRFV
                     PEKCVHTYSIDESFLDAGKENPEEMAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLE
                     SKKTKCGIARWRYEDVPNKLWKVHPLSKMWGIGGRMERNLNRMGISTVGQLAKYPLELL
                     EKKLGIMGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILLRDYTRREEIKAVLLEICE
                     EVARRARTHNKVGRTISLGIGYSKDELGGGFHRSKTIDLPTNITMDIYKYCLMLFNKFY
                     SGKTVRSISVTLSNIEDDVNQQLSLFEVDNEKRRKLGFVMDGIRSKFGSKAILRAVSYT
                     PAGTALHRAGLTGGHKS"
     gene            9385..9702
                     /gene="sunI"
                     /locus_tag="BLL41_RS21910"
                     /old_locus_tag="BLL41_21625"
     CDS             9385..9702
                     /codon_start=1
                     /gene="sunI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390032.1"
                     /locus_tag="BLL41_RS21910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21625"
                     /product="sublancin immunity protein SunI"
                     /protein_id="WP_060398793.1"
                     /transl_table=11
                     /translation="MEYVVMIIILLALFFIFTVFLNTRYSFDEKCLVLKFGLSKTEIPI
                     NQIVSIKESDKYGVADNIDYKIGMPYAQPDRIVIETTNKRFLVFLNGAHQFIQKYKKVS
                     V"
     misc_feature    complement(9899..9913)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.5"
                     /tool="antismash"
     gene            10001..10171
                     /gene="sunA"
                     /locus_tag="BLL41_RS21915"
                     /old_locus_tag="BLL41_21630"
     CDS             10001..10171
                     /codon_start=1
                     /gene="sunA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     glycocin: sublancin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390031.1"
                     /locus_tag="BLL41_RS21915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21630"
                     /product="bacteriocin sublancin-168"
                     /protein_id="WP_009967544.1"
                     /sec_met_domain="sublancin (E-value: 6.5e-46, bitscore:
                     144.6, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGA
                     VACQNYRQFCR"
     gene            10229..>11057
                     /gene="sunT"
                     /locus_tag="BLL41_RS21920"
                     /old_locus_tag="BLL41_21635"
                     /pseudo=""
     CDS             10229..>11057
                     /codon_start=1
                     /gene="sunT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230919.1"
                     /locus_tag="BLL41_RS21920"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BLL41_21635"
                     /product="sublancin transporter SunT"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 3.6e-36, bitscore:
                     114.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKKKQKYVHTKQFNSHDCGLACISSVLKFHNLNYGIDFLLDLI
                     GDKEGYSLRDLIIIFKKMGIKTRPLELQENKTFEALKQIKLPCIALLDGEEYGHYVTIY
                     EIRNNYLIVSDPDKDKITKIKKEDFESKFTNFILEIDNDSIPDKEKDQKKHSYFFKDIL
                     FRNKLIVFVILLTSLFVVALAVAGSFYIKFLVDLIIPRSLRESLITITLIFISMVLIRC
                     IFDFVRSYLIIKLSYKVDKEMSNVYFNKVTKLPINFFENREDGEVISRFNDGIY"
ORIGIN
        1 gaattcttaa ttagacaaga actcacacat gaatataata cgactgaaga aattgttaaa
       61 agagcttttt taaatgaaga atatagtgac aagaaagagc atttacttgt aaataggatt
      121 agaaaatcag atgcattcat tcctgaactt tcattggtcg cattaaatca agataaagat
      181 atcgtaggcc atgtcctttt atctaaaata aaaatcatag atggcgataa cactgtcgat
      241 tctttagcac ttgctccagt ttctgttgcg cctgattatc agaaaaaagg aattgggagt
      301 aaattaattc gtactgcgtt aaaaaacgca atagagcttg gttatcgttc agtaattgtt
      361 ttaggacata aagattatta tccaaggttt ggttttaagc cagctagctt atggaatata
      421 aaagccccat ttgaggtgcc tgatgaagtg tttatggctc tagaattagc agataattct
      481 cttgaagatg tgcaaggtat cgttaattat tcaaaagctt tttttgagta aaaattagat
      541 gattgattag atattgttaa aaagaggttg tcccaaaatt ttttaatctg tggatagcct
      601 ctttttgtgt aaagagccct aaaaataaaa agaaagccac aaagggcttt caattagcgt
      661 tcacatgcat agttgtcatg atccctgtcc attttagatt ggtaagcagg gtgggaacta
      721 ggcactccat tcggatattt ctttcttaac tctgtgcaat ttttaaatgt ttctgtttct
      781 ccagttgtct ctgaagaagc ttctttttca gttgtagtcg aagtttcact tgatgccttt
      841 ggagtagtag gctgtttagc tgccggttta gatgttgttg cttttttaac agcagtcgtt
      901 ttctccttca cacagccatc aaatcctcgg tcagtcacat acccattctt actccaaatt
      961 gaaagcttct ccgattttgc ttcctgctcg tctttcttaa atcggtctat gtatttcgta
     1021 tttggctcat atacatatgc tactctggcc aatccttctt ttagcaatgt ttcctgaaca
     1081 gatttgccat ccacataaac ataagctaag agtcttccgt atttatctct gcgatcgcct
     1141 ttgtcaaatt ccagctgcag cttaccactg ttaaccaatt ctttatttcg cttagaagca
     1201 tcttcaccgt acggttgaac acaagaattt ggtttcttcg tctcaggtgt gtctatgagc
     1261 aagtagcgca ctgtgtctac attgccgttg taactaactt taattgtgtc accatcaaca
     1321 gctctatcta gagtaacatc gaccaattcc tttttgtctt tttccttaga tttatcagca
     1381 gttttttctt tagtagattc cttttgttct gttttctttt ctacatctgt agacgcttgt
     1441 ggtgtttctt tttctgtgct tactttttca gaatcatttg agctgcaggc tgccaaagat
     1501 aagctcaaag tgaatgctgc aaaacctaat aatactttct tcaacttcat ttcccctttt
     1561 tagaatgttt gttctctatt tatatcggta aaccatcaat aattataatc ctatatccaa
     1621 ttattgtgaa tttaattttt ccttaatatc atcgcatttc tctttaaaat ttccaggcgc
     1681 atgttagaat ttcactgaat tcatagtcaa aaggggaatg tttttatgct tggaatcaaa
     1741 gttgaaaaat caaatgaaaa cttaattatc aattggcaac tatcaaaaat tgaaattcca
     1801 atcaaagaaa ttaacgatgt attccttgat ccaaactatg ggggagagga aaaaaatgca
     1861 gtaaggattg ggtttcctta tggctctacg gatagggtag tgattaaaac agcaaataac
     1921 acttatattt tgttcactac taatgctggt tcaattatga gtaaaattgt taattcttaa
     1981 acattaaaga tcatttgcca tgtgtttcgt gataataaaa agcaattata tatggggtga
     2041 tttatttgac taattttgtt cataagacag tcaacggttt gaaaagtctc ttagatgaaa
     2101 aaggggccat taaattattt tgttctgaag gaaatatcat ggagttcctt gttacatttt
     2161 cagaggacaa agcaaccctg aatgacatcc aaagctttga ggctaaacac cagctcaccc
     2221 ttccaaagga ttatcagaag ttcatgacac tacataatgg tgcgaaaatt tttcagattc
     2281 tttctgatgg ggagaatata ggtgggggac tttatttatt tagccttgaa gagattgaag
     2341 aggaactaaa atatgaggat ttatttgaag gtatcaatgg catcccaatt ggtcatttat
     2401 tagaaggttg tcatttgatg atcgacaaag ataaattgga taagggggat cccaactatc
     2461 tttttatatt acaattagga gagtatacac cattaaattt aaactttgag atatttctag
     2521 atcggtatat agaatccaat ggggcaactt tttgggaatg gcgatactat acagcagaaa
     2581 attattatag aacacgatag cacgatgtat aatatggtta tcttatttct tacaaagtct
     2641 ataggggctt aacaaagccc cttacttatt agtctggaat gacaaatcct ctaagttcta
     2701 cttcatggtg gtgttcttca ttgattaaat cttcaggaat gctcaaatcg tatttatggt
     2761 ttgtgaaaaa gtaaatgtat aaaagttttc cttgttcaat tgatggagct gtatccttat
     2821 cacgcataat agcacccaat ttaaagccca tgctctttcc ctgtattttt aattcctttg
     2881 taaattggct ttcgatttcg agaaccttta atttctgatc gagaatatta acaccttttt
     2941 caatttccct gtatgtgaaa tagtatttag acaatgcttc tgttttatat tcagcagcat
     3001 ttcttttttt caataaatct aggatttcat taatcgtttc ttgcggcaaa ttaagcttca
     3061 tattcttatc tccttgagtt tattttatga acatacatat atatcataca atatttgtgc
     3121 tattcaattg gagaaaatta ttaattatcg agaaatttgg ttttgttata aataacttaa
     3181 atgaaaggtg tatataatct tttgttaatt tagtcttaaa gtctcggttc ttgttggaag
     3241 ttctaaattt gtttatggta agatttagtt aaaaaaggaa aggagaatga aatgaaggta
     3301 tttgaagctg attcactgct ctctgaagcg gataaaagag ccaaagagta caaggaacta
     3361 aggtcgcaaa tggtgaagct caaaaaaacg tttaaagctg tagctgacct ggatgacagc
     3421 gagttttccg gtaagggtgc agacaacatc aaagcatttt atcatgatca tgtcggcgta
     3481 acagaccaat ggattgattt aattgacatg aaaattgctt tcttaagcag tatttctgcg
     3541 aaacttgaag atgctaagat gtctgatgct tacatagaag agtctttttt agaacatgag
     3601 ttggctaacg cctatacaaa atcaaaatcc attatgtctg aacaaaagaa agcgatgaaa
     3661 gacattctga acgacatcaa tgacattctt cctcttgaaa tattctcaac agaagatttc
     3721 aaagataaac tttcttctgc agatgacaaa cgtgaaaaaa caattgataa gctaaacaag
     3781 cttgatgagg atttaaaaac agaatacgct gaaacagaac caaacgaaca atttattcag
     3841 caagacttta aaaagctgca ggaatcaaca ggcaaaggga agaacgccac tcctattcac
     3901 tataacgcta aagcgtacag agaaagtgat atacataaga aaaaaggcga tattgagaag
     3961 cattctgaag cttacttaag cgtgaagaaa gaagaagaaa aagaacgaga aattaaagag
     4021 ttgaaaaaga aactcaatga cgatgtttcc gatcctgatg aatatttaga gattgctaag
     4081 aaagtaggat acgagaatct ggagccagct caagcacagc ttgcagtgca aattgaacaa
     4141 gcgaagcagc tagaaggagc aggagaaatt acctgggata tcgtaaaagg tgtaggagta
     4201 ggcttatatg atgtaggaaa agacactgta accggcatat gggacttcat caccgatcca
     4261 ggagaaacac tctcagcact ggggaatgca gttattcatc cagtgaaaac ctatgacgct
     4321 atttcagcag caatagaaga gtcatatcaa aaagatatgg tgaatgggga tgcctattct
     4381 agatcaaggt gggtaaccta tgctgttggt tcagtggctg tagcggtttt tggaacaaaa
     4441 ggtgcaggag ccattaacaa ggcagatgca gcgggcaaag taataaacaa agcaagtcag
     4501 gcaggaaaga aaattaagga cgtcaaaatt ccagatttgc tgccttataa tcctaagtac
     4561 gaccttgcta tggcaggggg cgtgccatac aatgtagtaa acggagaaag tttaaaaaat
     4621 aggctattgc aattagctgc ttcagcaaac ccacgttcag tattgccaag gactaggggg
     4681 aaatggatcg gtaaaccagg tgatgggcaa tggtattccg atatacctga agtaaacgcc
     4741 gcgacaaaag gcgaaccgat tacttttaaa gatgggcgtc ctgattttac tccatggact
     4801 gttgccaaca ttaaatttaa acctggagag ttaaacggaa ctaaaaagga ttttaggctt
     4861 gtatataaga aaattaaaga gatcaaaggt tttaagtctc aagcacaggc aaaaaaatgg
     4921 ctctcgcaac aggggcttac cccacaccat ttggatagta ccacaattca actggtacct
     4981 agtgtaatta ataacaaaat acctcatatt ggaggagcat ctgacctaag aggagggtat
     5041 taaaatgagc ttcactaaga tcgaacaaaa gttgaaagag tttacggtag atactgaaac
     5101 agatccttgc attaatacaa atgaaaaact caaagagatt gaaatgaata taggtaatca
     5161 attgccttcg gattataagg attttttgaa ggaatatgga ggatgttatt tagaaagtaa
     5221 aaaaacaaca gatgagattg agtatgacgt ttgttataaa cctatagaga aagacccttg
     5281 gatggggaag gacgatgata cacaattgtt agagggtttt tatggtttag cgaacgatca
     5341 caatagtctt ctgaaagcga tcgatacata ttctgatcgc tttcctagaa atattattcc
     5401 tattgcaagc tctgcaggtg ggaatgagat atgcatggat atcgataatg aaaagatact
     5461 gttttgggat catgaattaa gtcaaccaga taaagacttt ttcctgattg ccaattcgtt
     5521 tgaggagttt atcttaagtt tagttgatga accaattgaa actgatgata atgttagcga
     5581 tgttgaaatt gagattttgg acaatgattt tggaaagttg ctctttggga aggacaacta
     5641 gtctttattt ttttaaaacg ctcctctagg caaaagtatt gcaattgtat gagatggtgc
     5701 agaaacaagc atcctcaatc taaataaaat aatggtttta tccataaagc aatagtattg
     5761 caaataccat ttttatatgg ttataattag ggtaatgata caattatata attatttgat
     5821 gcatttgttt aattagagtg cataaaataa aaaaggccaa ggtgctacca acaccccggc
     5881 tatgtacaaa aagctgccca tcaaagggct tgctctgatg cagaaataaa cttagataga
     5941 cctaaccctt taaggtttcc agtctcaagg gaggtctatt ttttcttttg acttgacgtt
     6001 acaatcatca ttatattgaa gatcaacaag acggtattta atatcaaccc gccaaaattg
     6061 atcattatca ttaatgattc gtaaacagtc attttcccac cccctttcat aggggatgag
     6121 caagactccc ttgagtgagc aaactttatg tacaggaaat attatactat acataaatgt
     6181 ccttgtcgtt aaaaattcac aataaattga caatatgaaa agaacttgat aaatgcaggt
     6241 tctttttttt atttcaaata ttaaacaatg ccctttaaat gacgatattt aacatgtaaa
     6301 cttgtaccaa ggaggattta ggaaatgaag aagagaatta cgtatgtgct gcttgctctt
     6361 ctagcagtaa tagttttcgc tttcactgat tcatcgaaag ccaaagcagc agaagcaaca
     6421 ccactttatt atctgcaaat tacagggata acatcagatg gaaacgactt tgcttgggat
     6481 tacattaact ttaaccaaac caaagctcca agtgttttaa aaggtaataa acttcacgtt
     6541 agggctcgtt ttatggggta tacaaagtta acagttatca cgggtaaaga tgggaaaaat
     6601 ttgttatatg atggtacagc taggatgtac aaatccgatg ccattcttgg tcagaataag
     6661 gtggttatag gctgggataa atattttgag attccaatgg acgcacttca agataactct
     6721 atacagatta aagcattaag ctcaggcacc acttttgttt acagccaaaa aattgatttt
     6781 gaaagagagt gaaccaggtg attagcactt ctaattatga tgtaactaaa gatccaaagt
     6841 tcttagcatt ttgcagctta gctacagaag gatacggagg aaataactca gctcctagta
     6901 gatccattgg ttttagtgaa ttttgtgaaa aacttgagaa aaatgaagta gttaaacccg
     6961 agaacaaaaa aagctcactt gaactaaata agtctttaga ttattggaaa tcaagattta
     7021 atccgggagg taatctctct gcattagatt cacaggtatt agatattatc aaaaaagctt
     7081 acaacttagg catggttaac aaaaataata tgttgttgcg aaacgaagca attaatgctt
     7141 ataaaaattc aatttgaatt tgagatatag cccagcttag taaaagtgct gggcgttttt
     7201 atttattaga gttatttgct ttcagatact ctcgatgttc tttctccaac ttttcaatat
     7261 catctttaaa tatatagatc ttatattttt cctttgcttc tcttgacaga ttatgttcta
     7321 aatattcacg gagctttgtt tcacttttgg ttttatccat gccaattgta attgccggat
     7381 ctgttattcc aatttgaaaa ctaaataaac caaattcatc ccaagcactt tctaaaactt
     7441 cttcaggtgc ttttttgttt gattcgatta aagtaatacc cgatataatc aaaacaggaa
     7501 ctaaaatcaa aaatccaatt aacctcttct tcattcacca tctcactctc cctcgtcgat
     7561 aagaatctct cctttattaa tactacgtat atcattattg acctttatat tttattacca
     7621 acatttacat cgaacttttg ttcttgttta tgttgaataa agaacatttg ttctgttaat
     7681 attagatcaa taaaggagtg agtcgatatg cttagggatc gaggaacaat caagtggaca
     7741 tcaatgatgc ttccagaaca cttaacacaa cttaaacaag atttgattga tgtatcaaaa
     7801 attgaaaaac catccttaga tgaccaacaa attgaagaaa tggatcttct cgtctctgag
     7861 gcacttgaat ttaataaaga gttgaaattc aaactcttta acaatggatt cgttgaaaat
     7921 gtcactggca gagtccatta cattaatttt gaacaacaca ggcttcatgt aaaagaccag
     7981 aatgacaata caatttatat caacatgaat aacatcatag gagttacata caatgattga
     8041 ttactcacaa tttccacgta agaatatact ttgtgtcgat atgaaatcct tttatgcttc
     8101 tgtatcggct gtaacaatgg gacttaatcc tttaacatgt tatcttgctg ttgtagggaa
     8161 tacggagaga cagggaagtg tagtgttagc tgcatctcct gcacttaaaa aagattttgg
     8221 aatcaaaaca ggatcgagac tttttgagat acctgaagat ccaagaatac acattgtaaa
     8281 tccacaaatg aagcttttca tcagagtttc aactgagatt acaaagctgt tttacagatt
     8341 tgttcctgaa aaatgtgtgc atacgtattc aattgatgaa tcttttttag atgcaggaaa
     8401 agaaaatcct gaagaaatgg ccaaagcaat ccaaagcagc atgtggagag aatttggttt
     8461 gatgtgcaca gttggaattg gtgacaatat gttgcttagt aagcttgcac ttgacctgga
     8521 gagtaagaaa acaaagtgtg gtattgcacg ttggagatat gaagatgtgc caaataaact
     8581 ctggaaggtt cacccgttgt ctaaaatgtg gggaatagga gggaggatgg aaagaaacct
     8641 gaaccgaatg gggatatcga ctgtaggcca gttagccaag tatcccttag aattgcttga
     8701 aaagaagtta ggaataatgg gaaaccagtt gtattatcat gctcatggaa tcgatttatc
     8761 ggaaataggt gctccgttaa tgcaggggca gattagtttt ggtaaaagtc agattttact
     8821 gagggattac acaaggagag aagagattaa ggctgttctt ctggagattt gcgaagaagt
     8881 cgcaagaagg gcacgtacac ataataaagt tggtcgaaca atcagcttgg gaattggtta
     8941 cagtaaagat gagcttggcg gtggatttca ccgttctaaa acaattgatc ttcccacaaa
     9001 tattacaatg gacatttata agtattgctt gatgctcttt aataagtttt actcaggcaa
     9061 aacagtgaga agtatctcag ttacgttatc aaatattgag gatgatgtta atcagcagtt
     9121 gagtttattt gaagtggata atgaaaagag aaggaaactc gggtttgtca tggatgggat
     9181 tagaagcaaa tttggatcaa aagcaattct gagagcagtt tcttatacac ctgcaggtac
     9241 agcgcttcat agagctggac ttactggggg acataaaagt taagataaat ttaaacttat
     9301 ataacacatc tcttaaagtt tttttgtttt aaaaagttaa aaaacatggt aaaattatat
     9361 aaaaacataa gaaagagtga ttatatggaa tatgtagtta tgataatcat tttattagca
     9421 cttttcttta tttttactgt tttcctaaat acacgttata gttttgatga aaaatgctta
     9481 gttttaaaat ttggtttatc taaaacagaa attccaatta atcaaatagt tagtattaaa
     9541 gagtcagaca agtatggagt ggcagataat atcgattata aaattggtat gccatatgct
     9601 caaccagata gaattgttat tgaaactaca aataagcgtt ttctagtttt tttaaatgga
     9661 gctcatcaat ttattcaaaa gtataaaaag gttagtgttt gaatataaga aaagtacctt
     9721 cttacgatag aaggtacttt tttatatcta gtattattaa aaatttaatt aaatttttat
     9781 cattattaat tcaaaataaa tccattatga tcaattttgt ttagtatatt acaaccaatt
     9841 ctgtttatcg ataggtaata gaggtttttt cctgagattt atgaacaagt tatcttataa
     9901 ttttcaaaaa aaaataaaaa aatatggttg aatttagatt aatcttcctt tatattaaaa
     9961 aatgtaatcc ggattacaaa caaatgggga ggttttacaa atggaaaaac tatttaaaga
    10021 agttaaacta gaagaactcg aaaaccaaaa aggtagtgga ttaggaaaag ctcagtgtgc
    10081 tgcgttgtgg ttacaatgtg ctagtggcgg tacaattggt tgtggtggcg gagctgttgc
    10141 ttgccaaaac tatcgtcaat tctgcagata aaacatttat agagggaata ttttaaatat
    10201 tccctcatat ttaaagcggg gatcgaaatt gaataagaaa aagaaacaga aatatgttca
    10261 tactaaacag tttaatagtc atgattgtgg actagcttgt atctcgtcag ttttaaagtt
    10321 tcataacctt aactatggga ttgatttctt actagaccta attggggata aggaaggcta
    10381 tagtttaaga gacttaatta ttatttttaa gaagatgggg attaaaacta ggccacttga
    10441 attgcaagaa aataagacat tcgaagccct aaaacaaata aagctccctt gtatagcttt
    10501 gttagacggg gaggaatatg gacattacgt aacaatatac gaaatcagaa ataactattt
    10561 gattgttagt gatcctgata aagacaaaat aactaaaata aaaaaagagg attttgagag
    10621 caaattcaca aactttattt tagaaattga caatgattca attcctgata aagaaaaaga
    10681 tcaaaaaaaa cattcttact tttttaagga catacttttt agaaataaat taattgtttt
    10741 tgtgatttta ttgacctcct tgttcgttgt ggctcttgct gtagcagggt cgttttatat
    10801 aaagtttcta gttgatctaa ttatcccaag aagcttaaga gaatctttaa tcacaatcac
    10861 tttaatattc ataagtatgg tcttaataag gtgcatcttc gattttgtaa gatcatattt
    10921 gataataaaa ttgtcttaca aagttgataa agagatgtca aatgtttatt ttaataaggt
    10981 aacaaaattg cctattaatt tttttgaaaa tagagaagat ggagaggtaa tttctcgatt
    11041 caatgatggt atatata
//
