LOCUS       NZ_MNPQ01000042         4112 bp    DNA     linear   CON 11-AUG-2024
DEFINITION  Streptomyces albidoflavus strain OsiLf-2 Scaffold38_1, whole genome
            shotgun sequence.
ACCESSION   NZ_MNPQ01000042
VERSION     NZ_MNPQ01000042.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces albidoflavus
  ORGANISM  Streptomyces albidoflavus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Zhu,Y., Zeng,X., Zeng,J. and Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) College of Biology, Hunan University, 1
            Denggao Road, Changsha, Hunan 410082, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. FEBRUARY-2016
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001865795.1-RS_2024_08_11
            Annotation Date                   :: 08/11/2024 02:37:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,098
            CDSs (total)                      :: 6,022
            Genes (coding)                    :: 5,927
            CDSs (with protein)               :: 5,927
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 44 of 95
            Pseudo Genes (incomplete)         :: 58 of 95
            Pseudo Genes (internal stop)      :: 9 of 95
            Pseudo Genes (multiple problems)  :: 15 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:10:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4112
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MNPQ01000042.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4112
                     /collection_date="2015-11"
                     /db_xref="taxon:1886"
                     /geo_loc_name="China:Changsha, Hunan"
                     /host="rice"
                     /isolation_source="rice"
                     /lat_lon="28.4196 N 114.02870000 E"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces albidoflavus"
                     /strain="OsiLf-2"
                     /submitter_seqid="Scaffold38_1"
     region          1..4112
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4112
                     /SMILES="C(C)C(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4112
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[15:>4112](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      16..>4112
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        16..525
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: False, scaffold matched
                     GHGE: False"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BGV78_RS29885_PKS_AT.1"
                     /evalue="3.00E-51"
                     /label="BGV78_RS29885_PKS_AT.1"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="170"
                     /protein_start="0"
                     /score="166.5"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: (unknown)"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="MVSVARGVDEVRGLLAGWEGRIGVAAVNGPSSVVVSGDAGALDEF
                     VAACDASGVRARRVAVDYASHSAHVERIEEELAGLLAPVAPRSCDVPFYSTLSGGVIDT
                     AGLDAGYWYRNLRGTVEFEAGTRALLADGFRVFVEVSAHPVVATGMQETIEDAGVQAGV
                     VGTLRRD"
     aSModule        16..2646
                     /complete
                     /domains="nrpspksdomains_BGV78_RS29885_PKS_AT.1"
                     /domains="nrpspksdomains_BGV78_RS29885_PKS_KR.1"
                     /domains="nrpspksdomains_BGV78_RS29885_PKS_PP.1"
                     /locus_tags="BGV78_RS29885"
                     /monomer_pairings="mmal -> Me-ohmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     gene            16..>4112
                     /locus_tag="BGV78_RS29885"
     CDS             16..>4112
                     /NRPS_PKS="Domain: PKS_AT (0-170). E-value: 3e-51. Score:
                     166.5. Matches aSDomain:
                     nrpspksdomains_BGV78_RS29885_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (524-702). E-value: 4.5e-67.
                     Score: 217.5. Matches aSDomain:
                     nrpspksdomains_BGV78_RS29885_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (792-877). E-value: 3.2e-29.
                     Score: 93.1. Matches aSDomain:
                     nrpspksdomains_BGV78_RS29885_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (900-1327). E-value:
                     4.6e-182. Score: 597.6. Matches aSDomain:
                     nrpspksdomains_BGV78_RS29885_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="BGV78_RS29885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_256254012.1"
                     /sec_met_domain="PKS_AT (E-value: 5e-53, bitscore: 166.5,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.2e-68, bitscore:
                     216.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.4e-15, bitscore:
                     42.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 8.7e-229, bitscore:
                     745.6, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.1e-91, bitscore:
                     292.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVARGVDEVRGLLAGWEGRIGVAAVNGPSSVVVSGDAGALDEF
                     VAACDASGVRARRVAVDYASHSAHVERIEEELAGLLAPVAPRSCDVPFYSTLSGGVIDT
                     AGLDAGYWYRNLRGTVEFEAGTRALLADGFRVFVEVSAHPVVATGMQETIEDAGVQAGV
                     VGTLRRDEGGLERFALSLGEAWALGAEVDWETHYAGTRPRHVDLPTYPFRRDWYWLTPA
                     ESAAAAATGDPADGDFWSAVESGDLTRLSSVLEVTGDDVEAFGTLLPALASWRRRRTLR
                     STLDTWRHRVTWTALTGADDAVPAGRWPALVPAGHADDPWVHAVLGALTGRGLRVERHD
                     LTGDEDTAALTALLTGTGDTETDGAEGIAGVLSLLALDERPHPDHPAVPRGLATAKELL
                     HALSGTEASVWALTRGAVAVDSRDRLTSPVQAETWGFGRAVALELPDAWGGLADLPADP
                     DARSLARLATLLGGPEDQIAVRSSGAYARRLARMPLPEPARTTDADAELAVRAADDDGI
                     GGWGDHDTVLITGGTGALGAHVARSLAASGARHLVLTSRRGPGSPGAAELVAELEELGA
                     RVTVADCDVADRDQLARLLDSLPADLPLTGVVHAAGVLDDGVLDALTLDRFEAVLRPKT
                     LGTAHLHELTRDHDVTLFVLFSSIVGVLGNAGQANYAAANAYLDAVAEQRRAEGLPVTS
                     VAWGPWADAGMATADVLADRMSHDGLAPMDPVTAVAALRAAVAEGIPHATVLDVDWRAY
                     GTAMTAARPSPLVGDLPEVRRALEAAAATAPDTHALRDRLRGLAPAEQDRLLTDLVRAE
                     VATALRHSSPEAVDVNRAFKDLGFDSLTAVEVRNRITAATDVKLPTTLLFDHPNTTAVV
                     AHLRTLLLGDERPAAAPVVVSAGVTDEPMAVVAMACRFPGGVTTPEELWHLMAAEVDAV
                     STPPEDRGWDLDALYDPDPERHGTTYSREGGFIQDVAGFDPAFFGISPREALAMDPQQR
                     LLLETSWETFERAGIDPGTLRNSATGVFVGSITTDYQVRLGGAAAQEQLAGHLMTGNAS
                     SIASGRLSYTYGFEGPAVTMDTGCSSSMVALHLALQSLRTGECTMALAGGVTVMSTPEP
                     YVEFSRQRGLAADGRCKAFAEGADGMGFAEGVGLVLLERLSDARRNGHPVLAVVRGSAL
                     NQDGASNGLTAPNGPSQQRVIRQALANAGLAPGEVDVVEAHGTGTPLGDPIEAQAILAT
                     YGQDRDPARPLLLGSLKSNIGHTQAAAGVAGMMKMILAMRQGTLPKSLHISRPTSVVDW
                     SSGAVRLVTEPTPWPETGRPRRAGVSSFGISGTNGHLILEQAPAPEPAEDEPADPFTEP
                     SADDSADTVRSWMVSGKSRAAVG"
     CDS_motif       88..126
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0001"
                     /evalue="3.50E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="37"
                     /protein_start="24"
                     /score="13.8"
                     /tool="antismash"
                     /translation="AAVNGPSSVVVSG"
     CDS_motif       196..228
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0002"
                     /evalue="5.10E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="71"
                     /protein_start="60"
                     /score="12.8"
                     /tool="antismash"
                     /translation="VDYASHSAHVE"
     CDS_motif       334..381
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0003"
                     /evalue="6.90E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="122"
                     /protein_start="106"
                     /score="15.1"
                     /tool="antismash"
                     /translation="LDAGYWYRNLRGTVEF"
     CDS_motif       1588..1686
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0004"
                     /evalue="1.80E-13"
                     /label="PKSI-KR_m1"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="557"
                     /protein_start="524"
                     /score="43.0"
                     /tool="antismash"
                     /translation="TVLITGGTGALGAHVARSLAASGARHLVLTSRR"
     aSDomain        1588..2121
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BGV78_RS29885_PKS_KR.1"
                     /evalue="4.50E-67"
                     /label="BGV78_RS29885_PKS_KR.1"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="702"
                     /protein_start="524"
                     /score="217.5"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="TVLITGGTGALGAHVARSLAASGARHLVLTSRRGPGSPGAAELVA
                     ELEELGARVTVADCDVADRDQLARLLDSLPADLPLTGVVHAAGVLDDGVLDALTLDRFE
                     AVLRPKTLGTAHLHELTRDHDVTLFVLFSSIVGVLGNAGQANYAAANAYLDAVAEQRRA
                     EGLPVTSVAWGPWAD"
     CDS_motif       1969..2073
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0005"
                     /evalue="2.10E-21"
                     /label="PKSI-KR_m4"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="686"
                     /protein_start="651"
                     /score="68.5"
                     /tool="antismash"
                     /translation="LFVLFSSIVGVLGNAGQANYAAANAYLDAVAEQRR"
     aSDomain        2392..2646
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BGV78_RS29885_PKS_PP.1"
                     /evalue="3.20E-29"
                     /label="BGV78_RS29885_PKS_PP.1"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="877"
                     /protein_start="792"
                     /score="93.1"
                     /tool="antismash"
                     /translation="LRGLAPAEQDRLLTDLVRAEVATALRHSSPEAVDVNRAFKDLGFD
                     SLTAVEVRNRITAATDVKLPTTLLFDHPNTTAVVAHLRTL"
     aSDomain        2716..3996
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BGV78_RS29885_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="4.60E-182"
                     /label="BGV78_RS29885_PKS_KS.1"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="1327"
                     /protein_start="900"
                     /score="597.6"
                     /tool="antismash"
                     /translation="AVVAMACRFPGGVTTPEELWHLMAAEVDAVSTPPEDRGWDLDALY
                     DPDPERHGTTYSREGGFIQDVAGFDPAFFGISPREALAMDPQQRLLLETSWETFERAGI
                     DPGTLRNSATGVFVGSITTDYQVRLGGAAAQEQLAGHLMTGNASSIASGRLSYTYGFEG
                     PAVTMDTGCSSSMVALHLALQSLRTGECTMALAGGVTVMSTPEPYVEFSRQRGLAADGR
                     CKAFAEGADGMGFAEGVGLVLLERLSDARRNGHPVLAVVRGSALNQDGASNGLTAPNGP
                     SQQRVIRQALANAGLAPGEVDVVEAHGTGTPLGDPIEAQAILATYGQDRDPARPLLLGS
                     LKSNIGHTQAAAGVAGMMKMILAMRQGTLPKSLHISRPTSVVDWSSGAVRLVTEPTPWP
                     ETGRPRRAGVSSFGISGTNGHLILEQAP"
     CDS_motif       3202..3249
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0006"
                     /evalue="2.40E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="1078"
                     /protein_start="1062"
                     /score="16.5"
                     /tool="antismash"
                     /translation="GPAVTMDTGCSSSMVA"
     CDS_motif       3544..3564
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0007"
                     /evalue="9.90E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="1183"
                     /protein_start="1176"
                     /score="-3.5"
                     /tool="antismash"
                     /translation="APNGPSQ"
     CDS_motif       3625..3669
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0008"
                     /evalue="2.90E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="1218"
                     /protein_start="1203"
                     /score="13.0"
                     /tool="antismash"
                     /translation="VEAHGTGTPLGDPIE"
     CDS_motif       3730..3774
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BGV78_RS29885_0009"
                     /evalue="1.50E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="BGV78_RS29885"
                     /protein_end="1253"
                     /protein_start="1238"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
ORIGIN
        1 tcgggtctgg gcggcatggt gtcggtggcg cggggtgtgg acgaggttcg tggtctgctg
       61 gcgggctggg aggggcgtat tggtgtggcg gcggtgaacg ggccgtcgtc ggtggtggtg
      121 tcgggtgatg cgggtgcgtt ggacgagttc gtggccgcgt gtgacgcgtc tggtgtgcgg
      181 gcgcgtcggg tggcggtgga ctatgcgtcg cactccgcgc atgtggagcg gatcgaggag
      241 gagcttgccg ggcttctggc tccggtggcg ccgcgttcgt gtgatgtgcc gttctactcg
      301 acgttgtcgg gtggcgtgat cgacaccgct ggtctggatg ccgggtactg gtatcggaac
      361 ctgcggggga cggtggagtt cgaggcgggg acgcgtgcgt tgctggccga cgggttccgg
      421 gtgttcgtgg aggtgagtgc gcatccggtg gtggcgaccg gaatgcagga gaccatcgag
      481 gacgcgggcg tgcaggccgg cgttgtcggc acgctgcggc gcgatgaagg tggtctggag
      541 cggttcgcgc tgtcattggg tgaggcgtgg gcgctgggcg ccgaggtgga ctgggagacg
      601 cattacgcgg gcacccggcc ccgccacgtc gacctcccca cctacccgtt ccgccgtgac
      661 tggtactggc tgacgcccgc cgagtcggcc gcggcagcgg ccaccggcga ccccgccgac
      721 ggcgacttct ggtcggccgt cgagagcggc gatctcaccc ggctctcctc cgtgctggag
      781 gtcaccggcg acgacgtgga ggcgttcggc acgctgctgc ccgcgctggc gtcctggcgc
      841 cgtcgccgca ccctgcgctc caccctcgac acctggcgcc accgcgtcac ctggaccgcg
      901 ctgaccggcg ccgacgacgc cgtccccgcc ggccgctggc ccgccctcgt ccccgccggc
      961 cacgccgacg acccctgggt gcacgcggtg ctgggggctc tcacggggcg cggcctgcgt
     1021 gtcgagcgcc acgacctcac cggcgacgag gacaccgccg cgctcaccgc cctgctcacc
     1081 ggaaccggtg acaccgagac cgacggcgcc gaaggcatcg cgggcgtcct ctcgctcctc
     1141 gccctcgacg agcgccccca ccccgaccac cccgccgtac cgcgcggcct cgccaccgcc
     1201 aaggaactgc tgcacgcgct gtccggcacc gaagcctccg tgtgggcgct gacccgcggc
     1261 gccgtcgccg tcgacagccg cgaccggctg acctcgcccg tccaggccga gacctggggc
     1321 ttcggacgcg ccgtcgccct ggaactcccc gacgcctggg gcggcctcgc cgacctgccc
     1381 gccgaccccg acgcccgtag cctcgcccgc ctcgccacgc tcctcggcgg ccccgaggac
     1441 cagatcgccg tacgctcctc cggcgcgtac gcccgtcggc tggcccggat gccgctgccc
     1501 gagcccgccc gaaccaccga cgcggacgcg gaactcgccg tgcgggcggc cgacgacgac
     1561 gggatcggcg gctggggcga ccacgacacc gttctgatca ccggcggcac cggtgcgctc
     1621 ggcgcccacg tggctcgctc gctcgccgcc tccggcgccc gccacctggt gctcaccagc
     1681 cgacgcggac ccggcagccc cggtgccgcc gaactcgtcg ccgagctgga ggagctgggt
     1741 gcccgggtca ccgtcgccga ctgcgacgtg gccgaccgcg accagctcgc ccgtctgctg
     1801 gactccctcc ccgccgacct ccccctcacc ggcgtcgtgc acgccgccgg tgtcctggac
     1861 gacggtgttc tcgacgccct caccctggac cgcttcgaag ccgtcctgcg ccccaagacc
     1921 ctcggcaccg cccacctgca cgagctgacc cgcgaccacg acgtcaccct gttcgtcctc
     1981 ttctcctcga tcgtcggtgt cctcggcaac gccggacagg ccaactacgc cgccgccaac
     2041 gcctatctgg acgccgtagc cgagcagcgg cgggccgagg ggctgcccgt cacctccgtg
     2101 gcctggggtc cgtgggccga cgcgggcatg gccaccgccg acgtgctcgc cgaccggatg
     2161 agccacgacg gcctcgcccc gatggacccc gtcaccgccg tcgccgcgct gcgcgccgcc
     2221 gtcgccgagg gcatcccgca cgccaccgtc ctcgacgtgg actggcgggc ctacggcacc
     2281 gccatgaccg cggcccggcc cagcccgctc gtcggcgacc tccccgaggt ccgccgcgcc
     2341 ctggaggcgg ccgccgccac cgcgcccgac acccacgccc tgcgcgaccg gctgcgcggc
     2401 ctcgcccccg ccgaacagga ccggctgctc accgacctgg tccgcgccga ggtcgccacc
     2461 gcgctgcgcc actcctcacc ggaggccgtg gacgtcaacc gtgccttcaa ggacctgggc
     2521 ttcgactcgc tgaccgccgt ggaggtccgt aaccggatca ccgccgccac cgacgtcaag
     2581 ctgcccacca ccctcctctt cgaccacccc aacaccacgg cggtcgtcgc ccacctgcgt
     2641 accctgctcc tcggcgacga gcgccccgcc gccgcgcccg tcgtcgtctc cgctggcgtc
     2701 accgacgagc ccatggcggt cgtcgccatg gcctgccgct tccccggcgg cgtcaccacg
     2761 cccgaggaac tgtggcacct catggccgcc gaggtggacg ccgtctccac cccgcccgag
     2821 gaccgcggct gggacctcga cgccctgtac gacccagacc ccgaacgcca cggcaccacc
     2881 tacagccgtg agggcggctt catccaggac gtcgccggat tcgacccggc gttcttcggg
     2941 atctcgccgc gcgaggccct ggccatggac ccgcagcagc ggctcctgct ggagacctcc
     3001 tgggagacct tcgaacgcgc cggcatcgac ccgggaaccc tgcgcaacag cgccaccggg
     3061 gtcttcgtcg gcagcatcac cacggactac caggtccgcc tgggcggcgc cgccgcccag
     3121 gaacaactcg ccggccacct catgaccggc aacgcctcca gcatcgcctc cggccgcctg
     3181 tcgtacacgt acggcttcga aggccccgcc gtcaccatgg acaccggctg ctcctcgtcc
     3241 atggtcgccc tccacctggc cctgcagtcg ctgcgcaccg gcgagtgcac gatggccctg
     3301 gccggtggtg tcaccgtcat gtccacaccc gagccctacg tcgagttctc ccggcagcgg
     3361 ggcctggcgg ccgacgggcg ctgcaaggcc ttcgccgagg gcgccgacgg catgggcttc
     3421 gccgaaggcg tcggcctggt cctgctggag cggctctccg acgcacgccg caacggtcac
     3481 ccggtactcg ccgtggtccg cggctccgcc ctgaaccagg acggcgcgtc caacggcctg
     3541 accgccccca acggcccctc ccagcagcgc gtcatccgcc aggccctcgc caacgcgggc
     3601 ctcgccccgg gcgaggtcga cgtggtcgag gcgcacggca ccggcacccc gctcggcgac
     3661 cccatcgagg cgcaggccat cctcgccacc tacggccagg accgcgaccc ggcccggccg
     3721 ctgctgctcg gttcgctcaa gtccaacatc ggccacaccc aggccgccgc cggcgtcgcc
     3781 ggcatgatga agatgatcct ggcgatgcgg cagggcaccc tgcccaagag cctccacatc
     3841 agccggccga cctccgtcgt ggactggtcg agtggcgccg tccgcctcgt caccgagccc
     3901 accccctggc ccgagaccgg ccgtccgcgc cgcgccggcg tctcctcgtt cggcatcagc
     3961 ggcaccaacg gtcacctcat cctggaacag gcccccgccc ccgaacccgc cgaggacgag
     4021 ccggccgacc cgttcaccga gccctccgcg gacgactcgg ccgacaccgt ccggtcgtgg
     4081 atggtgtcgg gtaagtcgcg tgcggcggtg gg
//
