LOCUS       NZ_CP017708            13228 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Moorena producens JHB chromosome, complete genome.
ACCESSION   NZ_CP017708
VERSION     NZ_CP017708.2
KEYWORDS    RefSeq.
SOURCE      Moorena producens JHB
  ORGANISM  Moorena producens JHB
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Oscillatoriales; Oscillatoriaceae; Moorena.
REFERENCE   1  (bases 1 to 9648523)
  AUTHORS   Leao,T., Castelao,G., Korobeynikov,A., Monroe,E.A., Podell,S.,
            Glukhov,E., Allen,E.E., Gerwick,W.H. and Gerwick,L.
  TITLE     Comparative genomics uncovers the prolific and distinctive metabolic
            potential of the cyanobacterial genus Moorea
  JOURNAL   Proc Natl Acad Sci U S A 114 (12), 3198-3203 (2017)
   PUBMED   28265051
REFERENCE   2  (bases 1 to 9648523)
  AUTHORS   Ngo,T.-E.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2022) Scripps Institution of Oceanography,
            University of California - San Diego, 8615 Kennel Way, La Jolla, CA
            92037, USA
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 9648523)
  AUTHORS   Leao,T., Castelao,G., Korobeynikov,A., Monroe,E.A., Podell,S.,
            Glukhov,E., Allen,E., Gerwick,W. and Gerwick,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2016) Scripps Institution of Oceanography, UCSD,
            9500 Gilman Dr, La Jolla, CA 92093, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-JUN-2022
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:50:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,304
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 7,971
            CDSs (with protein)               :: 7,971
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 95 of 265
            Pseudo Genes (incomplete)         :: 172 of 265
            Pseudo Genes (internal stop)      :: 109 of 265
            Pseudo Genes (multiple problems)  :: 87 of 265
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:39:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1009249
            Orig. end                         :: 1022477
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017708.2.
            On Feb 2, 2023 this sequence version replaced NZ_CP017708.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerwick Lab Culture
            Collection, UCSD, SIO.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..13228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:8228](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8228
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(764..774)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            complement(1348..1833)
                     /locus_tag="BJP36_RS04560"
                     /old_locus_tag="BJP36_00510"
     CDS             complement(1348..1833)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191107.1"
                     /locus_tag="BJP36_RS04560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_00510"
                     /product="nitrate reductase associated protein"
                     /protein_id="WP_083373492.1"
                     /transl_table=11
                     /translation="MNKFENTMSNFFQFEADFVDSLRCIPMQVRMKLDTCGIKLKLSHW
                     QQFNQQERQQLVEIPCTTTESIQKYGDYVQHLVINYTGKPASILPVDPQPPWMNSELIP
                     STVAQKAMEFGVVVTPQQWAALIPIQRFALIKLTRPSHENKNFLPALKEFQLVIVDR"
     gene            complement(1830..2300)
                     /locus_tag="BJP36_RS04565"
                     /old_locus_tag="BJP36_00505"
     CDS             complement(1830..2300)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191106.1"
                     /locus_tag="BJP36_RS04565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_00505"
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="WP_071102150.1"
                     /transl_table=11
                     /translation="MRRLRQLFQKLRNAGKDENFLYGLNLFEGFVSKVLSLVMVVVILV
                     ALRSLVISLVKEITDPGAVVFSTTLFKIFGLFLNILIALEILENITAYLQKHVIQLELV
                     VVTSLIAVARKIIILDLKTTSGSQVIGLAIAIISLSISYWIIRSTSTRKHHK"
     misc_feature    complement(2596..2615)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 21.98"
                     /tool="antismash"
     misc_feature    complement(2816..2830)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.76"
                     /tool="antismash"
     misc_feature    2817..2830
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.07"
                     /tool="antismash"
     misc_feature    complement(2822..2837)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 26.19"
                     /tool="antismash"
     misc_feature    complement(2833..2848)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            3553..3996
                     /locus_tag="BJP36_RS04570"
                     /old_locus_tag="BJP36_00500"
     CDS             3553..3996
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BJP36_RS04570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BJP36_00500"
                     /product="hypothetical protein"
                     /protein_id="WP_229419722.1"
                     /transl_table=11
                     /translation="MNLKAVGLNPTDDPSHPWQCCGLTISNRLLRVLKSDTSDTGVGTE
                     GIEQDSSYFRNDVLSSNVLVSVGAKKGKISLLQGMFNLLKVFFDITAINQLPAVQGTPM
                     GLIGKTQPHWQVFRLVMDLKLKSVLVQQLLNPLWPMIRVASVE"
     misc_feature    complement(4065..4080)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.67"
                     /tool="antismash"
     gene            4275..4580
                     /locus_tag="BJP36_RS04575"
                     /old_locus_tag="BJP36_36435"
     CDS             4275..4580
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037966.1"
                     /locus_tag="BJP36_RS04575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_36435"
                     /product="HetP family heterocyst commitment protein"
                     /protein_id="WP_202970472.1"
                     /transl_table=11
                     /translation="MAKKLLSITSRPSTSRTSQPTSTSTSSEQVMTNEQLNQVVEAIIA
                     GKYSWACVLVLRFSGYNPLHYIPYRTYNRLIKENSQAGKHPVGKTPVSQSKVVSKV"
     gene            5001..8228
                     /locus_tag="BJP36_RS04580"
                     /old_locus_tag="BJP36_00490"
     CDS             5001..8228
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191099.1"
                     /locus_tag="BJP36_RS04580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_00490"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_071102148.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.4e-41, bitscore:
                     139.1, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 7.1e-158, bitscore:
                     526.0, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNIIRDSAEHGLPPSSINPDPASNLSVVLQSLTLDSVDPKLNYE
                     FSQSFDLDHFQLGDKILAYTRPELSQDSVQIEQDHQYLYLVCQGRIRLLGFDAEKQREV
                     SIQVLEEGETFGAYDLLGSLSYPYRAIAASSGQVARISLNQLLPWLERLPKWSEILGKQ
                     AQHRQCLIFFKTLTKLRTLNGVTLNQLLPYIVETRIEAGKSLVSSSQAGHCWLRSGQMG
                     TPEGTEENSSAPFPSIGDSWGYPMPVPTDWIAQTDVLLYQLPQEHWQAACAIAPVLANS
                     TESPDQENRQSAKNGDGKSPSDQGVVNKTHTPSPHPNQTRARPTRRVLEPPKQQTPLEP
                     SQSESFDFPKPAKQGWRFLRFWQRYPFIEQQSSSDCGAACLAMIGQYWGKRFALNSLRE
                     LAGVGRSGSSLKGLAKAAESLGFQASPVRASLGRLADRSNPWIAHWQGDHYIVVYQVKG
                     NKVLVADPAVGKRSLPRKDFLQGWTGYAMLLDPTPQLAVIPSDKRSLGPFWGLLWPHRS
                     VLLQIILATLILQVFGLISPLFTQIILDRVIVNRSLVTLNVFSIGLIIFGLWTIGVSLT
                     RRYLLDYFSNRLSLTFVSGFINHTLTLPLKFFESRQVGDILTRIQETGKIQGFITQQAI
                     ATWLDVVMVFVYIGLMLYYNWRLTVLVLATILPIAILTVIASPFLRQVSREIFKEAAQQ
                     NSMLVEMMSGVATVKTAASEQEVRWRWEDRLTNQLNVQFKGRNLANGLGSISSLINTLS
                     STALLWFGASLVINEQLTIGQYVAFNMLIGRVTGPILALVNLWDEFQEILVSVERLNDV
                     LSAKPEENPQEPMLVLPEIKGDVILEDVTFRYSEDEDRNILQNISLDIRAGETIALVGR
                     SGSGKSTLVKLLQGLYHPTNGRILIDGHDIRHISPQSLRSQMGVVPQECFLFSGTIVEN
                     ITLYRPDFSLDQAVEVAKLAEAHAFIQGMTLGYNTKVGERGSTLSGGQRQRIAIARALL
                     GNPKILILDEATSSLDTESERRFQQNLARISRDRTTFIIAHRLSTVRNADCILVLDRGV
                     LVEKGNHEELMDRQGLYYHLAQQQLDL"
     gene            9025..10629
                     /locus_tag="BJP36_RS04585"
                     /old_locus_tag="BJP36_00480"
     CDS             9025..10629
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191094.1"
                     /locus_tag="BJP36_RS04585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_00480"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_071102146.1"
                     /transl_table=11
                     /translation="MTQAKPINSPPEAATSRQGNVVLMPSGRLRPDYDQEAVASPSTEV
                     GTNTAHASNTSKWSTSIQTTLDQPPANLPRLLMLGGLSFCTIFVAWATFGQIDEVGKAH
                     GSLVPKGDVYKVDPIEPGKIASIKVKEGQAVKAGEVLVELDTDLATQEVERLEKVLNAD
                     RIRLVQMQGLLERTRLEAETREQITQAEVQAQQASIARAQASASAIRQQIIQHQLAKAA
                     NRERLQRMKLVQATAQELLQQRRANVVALKERLDNLKPLLAEGAISKEQVFQAEQSFRA
                     SQSAVTESKLQEGLSTREQIFQAQQSIRDREAATSQSRGELEQTLAEIKRLEAELEHRQ
                     ATGRTTRLEAQQKIQQLEVELTQIKARIAENETLVTTAKTRLKQKFLKAPVDGIISSLN
                     IGNAGEVVQPGRSIAEIAPQGAPLVLSASLPTREAGFINEGMPVQVKFDAYPYQDYGII
                     KGTVISISSDAKADQQMGSVYKVKVSLDRDYVIEEDEIIRFKAGQTANADIIIRRRRII
                     DFLLDPMRQLQKSGIDL"
     gene            10799..11233
                     /locus_tag="BJP36_RS04590"
                     /old_locus_tag="BJP36_00475"
     CDS             10799..11233
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037966.1"
                     /locus_tag="BJP36_RS04590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_00475"
                     /product="HetP family heterocyst commitment protein"
                     /protein_id="WP_071102145.1"
                     /transl_table=11
                     /translation="MTYRIPSANTKLEKVMDTDKFSEIEEAILDGKYSWACVLMLRFAG
                     HNPLDYMPSRTYSRLLKQNRDMILQKQASMMSNGTKALNARQRLSQIKDLAYVEKLGKK
                     TSHVHGGDQEEWFISELEEEESMLKKLADYLRQDCLVSLW"
     gene            complement(11712..11846)
                     /locus_tag="BJP36_RS04595"
                     /old_locus_tag="BJP36_36440"
     CDS             complement(11712..11846)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BJP36_RS04595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BJP36_36440"
                     /product="hypothetical protein"
                     /protein_id="WP_267875497.1"
                     /transl_table=11
                     /translation="MGRGGRVGRVGRVGRWAEGRVWEVWEEGGVWGVWGVWGDGEMGR"
     misc_feature    12291..12304
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.13"
                     /tool="antismash"
     gene            12420..12749
                     /locus_tag="BJP36_RS04600"
                     /old_locus_tag="BJP36_00470"
     CDS             12420..12749
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191086.1"
                     /locus_tag="BJP36_RS04600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BJP36_00470"
                     /product="hypothetical protein"
                     /protein_id="WP_071102144.1"
                     /transl_table=11
                     /translation="MLISDLNHLESVDASQVQGASAGISLNNNIVTSLSTSSIVTEIDT
                     LSVDISATVNIAGVSASSSSQGEGSSASGFNVGTKQQSVAVTIEGPDGTASKSVGIASS
                     AIGIY"
ORIGIN
        1 ttgttcattg cccaatttcc actcaccatc agaccagctc accattcgtg aaccacggtc
       61 tagctgcgtt ttggtaatgg aatcagacaa gttgctggga atctttactg agcgcgatat
      121 tgtccggcta acagctaatg gaattaactt tcaagaggta acagtagctg aggtcatggc
      181 acagcctgtg attacctttc ctcaaactgc ttgtcgggat atctttgccg ccttatttat
      241 atttcgtcgg tatcggattc gtcacttacc aattgtcgat gatcacggtc agctagtggg
      301 tgttgtctca ccagagcgaa ttcgccaggt attgcgacca gccaatctgc tcaagttacg
      361 gcgggtatca gaagtgatga ccccccaggt ggttaatgcc cttccaactg tttcagtatt
      421 gagtttagcc caactcatga ataagcaccg agttagttgt gtggtcatta cctccgacca
      481 tggagaggat agctgtatgc cagtggggat tgtcacagag cgggatattg tccaatttca
      541 gtctctgaag ctgaatttgt ctaaagtcca ggctcaggat gtgatgagta cacctctatt
      601 tctgctgagt cctgaggatt ctctatggac agctcatcag gaaatgcaaa agcgtcgtgt
      661 gcggcgattg gtggtgtctt ggaattgggg taaagaaatt ggtattgtca cccaaacaac
      721 cctgctgcga gtgtttgacc caatggaaat gtatgggatt gtggagacat tgcagcaaac
      781 cgttgagcaa ttagacccag ccaaagaatc cgtgaacaaa tcccacaaag ccattccccc
      841 tggtgaagaa tctggggagg tggcagaaaa tgcgatcgct ttatcaggag ataggtttaa
      901 tcataaatca gagccaccca acacagacca aaacacagac caaaacagag aactaaccct
      961 gatgttgtca caaccgatag aggatcaagg attaaatact tcccaacagt gtgaggcttt
     1021 caactatccc aaaagtaact tgggtaccag tcaagattat caactgtgta tcctgctcaa
     1081 cactatacaa gatatcctag aaaacctagt taaccagtct aacctatcat cagaattacg
     1141 tcaaagccag cttaattcag ccttagataa tgttgatcaa atgcgagagc taatacagaa
     1201 agcccaaaac cagtcttgtc gggatcaggt cgtggtagat caggtaggta cttctagcag
     1261 ttaaggtaat ccagatttaa tgggcatatt tgacttggtc tgaatttgtt gaacggtgtt
     1321 cgcccgaggg atcccttgtc gggtaggtta acggtcaaca ataactaatt ggaattcctt
     1381 gagtgctggt aaaaagtttt tgttttcatg actagggcgc gtcagcttaa tcagggcaaa
     1441 acgttggata ggaattagtg ctgcccactg ttgaggagtt actacgactc caaattccat
     1501 agctttttgc gccacggtag atggaattag ctcagaattc atccagggag gttggggatc
     1561 aacaggtaag atagaagccg gttttcctgt atagtttata actaggtgtt gaacatagtc
     1621 tccatacttt tggattgact cggtcgtagt gcagggtatc tctaccagtt gctgacgctc
     1681 ctgttgatta aactgctgcc agtggctcag tttaagtttg atgccgcaag tgtctaattt
     1741 catccgcact tgcataggaa tacagcgtaa agaatccaca aaatccgctt caaattggaa
     1801 aaaattactc atagtattct caaacttatt catttatgat gttttcttgt gctagtactc
     1861 cgtataatcc agtaactaat tgatagagat ataatggcaa ttgctaatcc aattacctgg
     1921 cttccagaag ttgttttaag gtcaagaatg atgattttac gggcaactgc aattaaagac
     1981 gtaacaacaa ctaactcaag ttgaataaca tgtttttgca gataagcggt tatattctct
     2041 aaaatttcta gtgcaatgag aatatttaga aacagtccaa atattttgaa aagtgttgtg
     2101 ctaaaaacta ctgctcctgg atcggttatt tctttaacta gtgagataac taggcttcta
     2161 agtgcaacca atattaccac caccatcacc aaagagagaa ctttggaaac aaaaccctcg
     2221 aacagattta gcccgtataa aaaattctca tctttgcctg catttctaag tttttggaag
     2281 agttgtctta accgcctcat aggaataggt tagtaaaaag acagatgatg gttgatttta
     2341 acacttcgac ggggtattaa cctaacagat gagtcaatgt tgtggttgca tttccctgat
     2401 gccacccccc ttgttcaact atcacaatct ctatgacgtc ataaagccaa gacgatcggc
     2461 aagagggagg ccgttgctga ttcatgcaat gattgagggt aattggtaat tgggaattgg
     2521 taattgggaa ttgggaatta ggaattggtc actgacccga ctaaacttga cggagttcct
     2581 tagttcagta accctagagg gagaatagac ccgaagaggg gacagagggg gcagattgac
     2641 catgatattt gtcacaaaaa tttacattgg gggtggagca agatttagta aacatatagt
     2701 aaagatttca ttaaagttcc ctttaggttg aggagggtga catatagctc ctagatttcc
     2761 atgtcagctt actcaagatt acgtgaagat tgtgtgaaat ttgcgtaaat ttggtaattt
     2821 tgtgaaaaaa atatgaataa aatgtgaatg tagaaaagaa atgtgagttt tcgcttgaca
     2881 attacccctt gaatttacta gatttccttt tgtagatgaa caaagtttca cgttcaagat
     2941 catcggcgtg aaatataact tcatcaacct tacagccaaa gcaatccaat tttggatttg
     3001 tcttttttga aagaatcaaa gagggtaact atgctcaagg tagtgatttt attgtttcag
     3061 ctcactcggc cagctttttt aatagaactt gtaactactt caggctagac gccatcgcct
     3121 ttatggagct aaggctgagt agatatactc aaagcgctca gctcagcatt tagccacaca
     3181 gaaactctag ataaaaactc tagaatattg gtaaattcag ctgctcatct gtttcggtct
     3241 ctaggtgttt ggggatttac tccagacaac agaaaagctt tctgtctaaa gacagaggaa
     3301 tttaaagagg gtttgtattt gttgttgcgg gtgcctgaaa taggatcaat ctttcactac
     3361 atctgcgctt agtctatgta ggcaagcctc tggtattgtg aaaaattagg tgtcacatta
     3421 ggtgtcacaa cagcatcaac tttgaaaaaa tcatccaaca gactcatgaa cataccatta
     3481 ggaaagcgga agccatcgcc gcattctcta atccagcttg ttactaagag cttttcaggg
     3541 gtgcttagca aattgaacct gaaagcagta ggtttaaatc ccactgacga ccctagccat
     3601 ccctggcagt gctgtgggtt aactatctcg aaccgattgc tgagggttct taaatctgac
     3661 acctccgaca ctggtgttgg cactgaaggc attgaacagg atagcagcta tttccggaat
     3721 gatgtgctat cgtcaaacgt attagtctcg gtgggagcaa aaaaggggaa aatcagctta
     3781 ctgcaaggga tgttcaatct cttgaaagtc ttctttgaca taactgcaat taatcagctg
     3841 cccgcagtgc aagggactcc tatggggctg attgggaaaa ctcaacccca ctggcaagta
     3901 tttagattgg tcatggatct caaactcaag tcggttctag tccagcagtt gttaaatcca
     3961 ttgtggccaa tgatcagggt ggcatctgtt gagtgagtga gcctcaacag atgccaccga
     4021 ttaatccttg ggttgagagt ttgttattgt cccccacgct agtttgtacg cccaaagcgt
     4081 gtttatacaa agcgtgttta tacagagttt agacaaagcg ctatgagcta aaagcccata
     4141 gtcagctatg tggataaatt gcaataatcc aaaacccgtt ccaaggctta attattaccc
     4201 cggtgaactg gcaacttccc tttgccagtt gggtatagac cattacaaga tgtcgttaat
     4261 tctgggtgac taccatggcc aaaaaattgc tttcaataac tagtagacca tcaactagta
     4321 gaacatctca accaacgtca acctctacga gctctgaaca agtcatgact aatgagcagc
     4381 ttaaccaggt cgtagaagct atcatcgcag gcaagtattc ttgggcttgt gtgttagttc
     4441 tgcgtttttc tggctacaac cccctccatt acatccctta ccgaacctac aatcggttaa
     4501 tcaaagaaaa tagtcaagct ggtaagcatc cagttggcaa aacccctgtc tcacaatcaa
     4561 aggttgttag taaggtctag tcagctaagc gggaaagttg ggagattcat caagtctgaa
     4621 cttaaccacc tgcctaggtc agtttgatgg attattgatg cagagcctag ttttcaacgt
     4681 cttcccgttt aatctgttga actccactcg catcaaccgc gctatctgct tacatccagc
     4741 tgacatctat tggcttaatg agctggctca gacttgacct cctatctccc tttccccaac
     4801 ccttatgggt ttactttaca ccagatgcta taggaataat ttaaagtttt aatgaagttt
     4861 acttaaagtt ttggtgaagt ttttgtgaat tattggtaac tagccggtta atgacttgac
     4921 aaaccctaat ttattagcta actaaatgta gttaattgta aatggttaat tgttaaaaat
     4981 caattttcaa tgtttaacat atgaagaata taatcaggga ttcggcagag catgggttac
     5041 caccgtcttc tatcaaccca gaccctgcta gcaatttatc agtggtgctt caaagcttga
     5101 ccctagactc agtcgatcca aagctcaact acgaatttag tcaatcattt gaccttgatc
     5161 acttccagct aggggataaa atccttgcct atactagacc agagctatcc caagattctg
     5221 ttcagattga acaagaccac caatatcttt acctagtttg tcaggggcgg atacgactac
     5281 taggctttga tgctgaaaaa cagcgggagg tttccataca ggtactcgaa gaaggggaaa
     5341 cctttggtgc ctatgatctg ttaggtagtc tatcctatcc ttacagagcg atcgcagcga
     5401 gttcaggaca agttgctcgt atttcgctaa atcaactctt gccttggttg gaacggttgc
     5461 caaagtggtc ggaaatcctg gggaaacaag cccagcaccg gcaatgccta attttcttca
     5521 agactttgac caaattgcgt acgcttaacg gtgttaccct aaatcaactt ttgccctaca
     5581 tcgtagaaac acgaattgag gctgggaagt ctctggtgtc ctcttcccaa gctgggcatt
     5641 gctggctacg tagtggtcaa atgggaacgc cagaaggaac agaggaaaat tcctctgccc
     5701 catttcctag catcggagac agttggggat atccgatgcc agtaccaaca gattggattg
     5761 cccaaactga tgtattgctt taccagctgc ctcaagagca ttggcaagcg gcttgcgcga
     5821 tcgcaccagt tttggctaac tccaccgagt caccggatca ggagaacagg caaagtgcta
     5881 aaaatggtga cggtaaatct ccatcggatc aaggcgtagt taacaagacc catactccgt
     5941 caccacatcc aaaccagact agagcacgac cgactcggcg agttttagaa cctcctaagc
     6001 aacaaacgcc ccttgaaccg agtcagtcag aaagctttga ttttcccaaa ccagcaaagc
     6061 agggttggag attcctaagg ttttggcagc gctatccttt tattgagcag caatcttcct
     6121 ccgattgtgg tgctgcctgc ttagccatga ttgggcagta ttggggcaaa aggtttgctc
     6181 tcaactccct gcgggaactg gcgggagtgg gtcgttctgg ttcatccctc aaaggtttag
     6241 ctaaagcagc ggaaagttta gggtttcaag ctagtccagt cagggcttct ttaggtcgct
     6301 tagcggatcg gagtaatcct tggattgctc actggcaagg agatcactat attgttgtct
     6361 accaggtaaa gggtaataag gtactcgttg ctgatccagc agtggggaag cgatcgcttc
     6421 ctcgcaaaga ctttctccaa ggttggactg ggtatgccat gctattggat ccaactcccc
     6481 aactagcagt gattcccagc gacaaacgtt ctctagggcc gttttggggg ttgctatggc
     6541 cccatcgctc agttctgctg caaattatcc tcgctaccct catccttcag gtttttggtc
     6601 tgattagtcc cctatttacc cagatcattc ttgaccgggt gattgtcaac agaagcctgg
     6661 tcactctcaa tgtcttctcc attggtctaa tcatctttgg cctttggacg attggggtta
     6721 gtctaaccag acgctacttg ctggactact tttccaatcg gcttagtctc accttcgtca
     6781 gtggcttcat taaccatacc ctgaccttac ccctcaagtt ttttgaatcc cgccaagtgg
     6841 gggatatcct gacccgaatt caggaaactg gaaaaattca gggttttata actcagcaag
     6901 cgattgctac ttggctggat gtggtgatgg tatttgtcta tattggactg atgctctact
     6961 acaattggcg tctaactgtg ctggtgttgg caactatttt acccatcgcc atcttgactg
     7021 tgattgccag tccctttcta cgccaggttt ccagggaaat ctttaaagaa gcagcccaac
     7081 aaaattctat gttagtggaa atgatgtcag gggttgctac tgtcaaaacg gcagcttccg
     7141 aacaagaagt gcgctggcgc tgggaagatc gcctcaccaa tcagttgaat gtacaattta
     7201 aaggtagaaa cctggctaac gggttagggt caattagttc tctaattaat actctcagca
     7261 gcaccgcttt gctttggttt ggcgctagct tagtaattaa tgagcagctt actatcggtc
     7321 agtatgtcgc gtttaatatg ctcattggcc gagtcactgg tccgattttg gctctagtga
     7381 atctttggga tgagtttcaa gaaatcttgg tgtctgtaga acggcttaat gatgtcttat
     7441 ctgctaaacc agaggagaat cctcaagaac cgatgctggt tctacctgag attaagggtg
     7501 atgtaatatt ggaagatgtt acctttcgat actcggagga tgaggatcgc aatatcctac
     7561 agaatatttc cctagatatt agggctggag aaaccattgc tctagtgggg cgtagtggct
     7621 cgggcaagag taccttggtg aagctactcc aaggtctcta tcatccgact aatggtcgta
     7681 ttttgataga tggacacgat atccgccata tttcccccca atccttgcgt tctcaaatgg
     7741 gtgtggtacc ccaagaatgt ttcttgttct cggggacaat tgtcgaaaat atcactctct
     7801 accgaccaga tttcagcctc gatcaagccg tagaggtagc taaactagca gaagcccatg
     7861 cctttattca aggtatgact ttgggatata atactaaagt cggagagcga ggctctaccc
     7921 tttctggtgg acaacgacaa aggattgcca tagcccgagc cctattgggc aaccccaaga
     7981 ttctgattct ggatgaagcg actagctctc tagatacaga atccgagcgc cgctttcaac
     8041 aaaacttagc tcgcattagt cgcgatcgca ctaccttcat catcgcccac cgcctctcta
     8101 cagtgcgcaa tgctgactgc atccttgtcc tagaccgagg tgttctggtc gagaaaggca
     8161 accatgagga actcatggat cgtcaaggtc tttactatca tctagcgcag cagcagctag
     8221 atctttaata gatcgttaac aagtgctgat tagtcattag ttattagtta ttagttattt
     8281 agtcattagt catttgtaaa taccaacaga ccctggttag tcacaaagga ctaatgacta
     8341 agaaaatttg aagctaccta tcactatatc aaactatgtg aaaatttgcc aatttggcaa
     8401 cacagtgatg tgaaaaggac taatgactaa gcacaaataa aatttgaagc tagctatcac
     8461 tatgtcaagc tttgtaaaaa gctgccaaaa atctgccaat ttagcaacat actcagatga
     8521 aattgatctt ttatcagaaa agtggaaaag cgctgtgaga atagaagggg agaaaaacgt
     8581 gttcagatat ggctccacac ccatctcgta acaaccgttt agaatcccct gagaatcccc
     8641 catgatgcac actgcggcag agttaaattc caattccttt gtttaattca ctcccatgca
     8701 ccccaccaaa ggtgagacgg aggtaacttg gagagcctaa ccctaaggtc agtcctgctc
     8761 aagtccacag ctcacagcgt gctcagccaa gaggtttgac cgaccgacgg ctacttaaag
     8821 gggaagggtt taagttctga tctcggggtg catttaagct atctggaaaa ggggcaagta
     8881 gagtcaggta atgccaagga tttcggtctg cccaacaata ccaaactcgc cccgcgaagc
     8941 taagggcagc aagagaattg gtcgctgact ttcccaaaac attggtcatt tccctaaaat
     9001 gttaatttag cgtgatctac caccatgaca caagcaaaac caatcaactc cccacctgag
     9061 gcggctacca gtcgtcaggg taacgtcgtt ttaatgccat caggtcgcct gagacctgat
     9121 tatgaccaag aggctgtagc ctcacctagc acagaggtag gaaccaatac tgcccatgct
     9181 tctaatacgt ccaaatggtc aacctcgata caaactaccc tcgaccagcc tccggcgaac
     9241 cttccccggt tgctgatgtt aggtgggtta agcttctgta cgatatttgt ggcttgggca
     9301 acgtttgggc agattgatga agtcggtaaa gctcatggaa gcttagtgcc aaaaggcgac
     9361 gtttataaag ttgacccgat tgagccaggc aagattgcta gtattaaggt caaagaaggg
     9421 caagcggtta aagctgggga agtcttggtg gaactggata cagaccttgc cactcaggaa
     9481 gtagagcgtt tagagaaagt gcttaatgca gaccggatta ggttagttca gatgcaagga
     9541 ctgcttgaaa gaacccgtct ggaagcagaa actcgtgagc agattaccca agcagaagtt
     9601 caagcacaac aagcttcgat cgcacgagca caggctagtg catcggcaat tagacaacaa
     9661 atcattcaac atcagttagc taaagctgct aatcgcgaac gattacaacg gatgaagctg
     9721 gtgcaggcga cagcccaaga actgctccag caacggcggg ctaatgtagt ggctctcaag
     9781 gaacgactcg ataaccttaa gcctctgttg gcagaaggag caatctccaa agaacaggta
     9841 tttcaagcag aacaaagctt tcgtgctagc caaagtgctg ttaccgaaag caaactgcaa
     9901 gagggtttga gtactcggga acagatcttt caagctcagc aaagtatacg cgatcgcgaa
     9961 gcggcgacta gccaaagccg aggtgagcta gaacagactt tagcagaaat caagcgacta
    10021 gaggcagaat tagagcatcg gcaagccacc ggacgcacga ctcgcctgga agcacagcag
    10081 aaaattcaac agttagaagt agaactgacc caaatcaaag ccagaattgc tgaaaatgaa
    10141 accctggtca ctactgctaa aaccaggctc aaacagaagt ttctcaaagc gccagtggat
    10201 gggataatat cttccctcaa cattggtaat gctggtgaag tcgtgcaacc gggacgtagc
    10261 atcgccgaaa tcgctcctca aggtgccccc ctagtgctat cggcaagttt gcctactcgc
    10321 gaggcagggt ttattaacga aggaatgccg gtgcaagtaa aatttgatgc ctatccctat
    10381 caggattacg gcatcatcaa aggcacagtg atttccatct cttccgatgc taaagccgat
    10441 caacaaatgg gttcagtcta taaggtgaaa gtgtccctcg accgtgacta tgtgattgaa
    10501 gaagatgaaa tcatccggtt taaggctggt cagaccgcca atgctgacat catcatccgc
    10561 cgccgtcgga ttatcgattt cttattggat ccgatgcgac agctgcaaaa aagtggcatc
    10621 gatctatagt gctctgttct ctcattttca tcagtgactg atcgttttcc gttgacggtt
    10681 aacagttgac cgttaattct tagtagtcaa cttgctagta attccttgaa atctgcaagt
    10741 taactgcacc ccagcttata aactattgac ctttagactt acaggataag aaataaccat
    10801 gacctatcgt attccttctg ccaataccaa gctcgaaaaa gtgatggata ccgataagtt
    10861 tagcgagatc gaggaagcca tccttgatgg caagtattct tgggcttgtg tgttgatgct
    10921 acgctttgcg ggccataacc ccctagacta catgccttct agaacctaca gccgcttgct
    10981 caaacaaaac cgggatatga tcttacagaa gcaagctagt atgatgagca atggcactaa
    11041 agccttgaat gcacggcagc gtttgagcca gatcaaagat ctggcttatg tagagaagct
    11101 tggcaagaaa acctcacacg tccacggtgg cgatcaagaa gagtggttta tcagtgaact
    11161 ggaagaagag gaatccatgc tcaagaagtt agccgattat ttacggcaag actgtttggt
    11221 ttccctgtgg tgagcaggta taatcatttt cctacccatc ctccttgtca ttaccagcca
    11281 ctgcttacca ccacaccaga tcagactaag cctgtggtca aagtaattca ttaataaccg
    11341 ccttgtccat taggtgcgct ttccgcatta agaattaaat tcgccacggg tcgcacctgt
    11401 tgatggacga ggcgtttttt tatctgatca cagaccggaa tgaaacagcc ctagtaaaaa
    11461 cagccctagt actctgtcaa tttagttttt agggttaggg ctaggtggca cggttaatgg
    11521 ttaagcgggc ctaatctaag ggtttaagcg tggctaatcc ctagttaacc taagttcgac
    11581 gtaaccctga aagccttaat ctaccctagt tgcccaaccc ttgtatttct tactctttcg
    11641 tcaagcgatc gcttatatcg aactcaggtt ataccaattc ttagcgctca cacggctaca
    11701 catcaataat ttcatctccc catctcccca tctccccaca cgccccacac gccccacacg
    11761 cccccctctt cccacacttc ccacactctc ccctctgccc atctccccac acgccccaca
    11821 cgccccacac gcccccctct ccccacactt cccacactcc ctgctccctg ctccctgttc
    11881 cctgctccct actccctact ccctactccc gcgcaaccca gaacgaaagt acctacttta
    11941 gttctgggag aattcaatca tcaatatcaa gccaaagtgg cgttcaaatt tttaattaga
    12001 ttatatataa taacccgctc attactaata acttacttca ataaaacccc atcaagattc
    12061 ccaatctctt tgaactaatc cagaatcggt tactggtcac tctttagttg tgggaggatt
    12121 caaccatcaa tatcaagcca aagtggcctt aaaattttta attagattag atataataac
    12181 cgtttcatta ctaataattt acttcaataa aaccccatca agattcccaa tctctttgaa
    12241 ctaattcaaa atcggttact ggtgactaga tacttttagt aaattcagtg attttaaaaa
    12301 aaaaaggttt aagccaaatt ggcgagacat gcctagggga atctggctat agttatatca
    12361 acagctaaaa caaagctgat tcaaacaaac aaaatcaaca acaaagaagg agaaaatcaa
    12421 tgcttatttc tgacctaaac catctagaat ctgttgacgc tagccaagtt caaggtgctt
    12481 ctgctggaat ctcattgaac aacaatatcg ttaccagttt aagcacctcc agcatcgtca
    12541 ccgaaatcga cactttgagt gttgacattt cagctaccgt aaacattgct ggtgttagcg
    12601 cttcaagctc tagccaaggt gaaggttcta gcgctagcgg tttcaatgtt ggtactaagc
    12661 aacaaagtgt agctgtcact attgagggcc ctgacggaac tgcctccaag tctgtaggta
    12721 ttgcttcctc tgctatcggc atctactagt acttagtcag gaaaagttcc tgaataagta
    12781 acttgtaaac ctgggtgtcc tcatatccta atagaatgca gtaaattcag ttctatatca
    12841 ggatgagtac actcaggttt acgtttgatt gcttcagtgt gattgcttca ggatagtaaa
    12901 tcaccagcca atagttatat caaaccggaa atcaataggt ttattgccaa attggcgaga
    12961 caaacatttt ggaaatttgc tagtttaatt ggagtgtaaa atcaacaagt ttattaacac
    13021 taaaacagcc aaaaggagaa aagtaaataa tgattatttc tgatctaaat catttagaaa
    13081 ctattgctga agaccccaag gttgtgggtg gctctcggta ctacccacct cactacaact
    13141 ctttcgtata taacggatat atcaacgtcg gtgccaacat tcaagggcac gtagctaggg
    13201 ttgacggcga cgcaagagcc tttggtaa
//
