LOCUS       NZ_KV804811            15861 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Rothia sp. HMSC065G12 Scaffold56, whole genome shotgun sequence.
ACCESSION   NZ_KV804811
VERSION     NZ_KV804811.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Rothia sp. HMSC065G12
  ORGANISM  Rothia sp. HMSC065G12
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Micrococcaceae; Rothia.
REFERENCE   1  (bases 1 to 106207)
  AUTHORS   Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R.,
            Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A.,
            Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) McDonnell Genome Institute, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 144x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001811455.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 12:58:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 4 of 44
            Pseudo Genes (incomplete)         :: 39 of 44
            Pseudo Genes (internal stop)      :: 2 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:45:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 31696
            Orig. end                         :: 47557
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV804811.1.
            The WUSC is a large strain collection isolated from clinical
            samples. Each sample is associated with metadata, including source,
            isolation site, 16s rRNA, metabolic, and other phenotypic
            information. The goal is to sample an adequate number of important,
            yet minor species, further adding to the catelogue of sequenced
            bacterial genomes and improving the diversity of the genomes
            available to the public. WGS will be preformed on approximely 550
            isolates. Samples were selected based on RDP analysis at the genus
            level. This is a reference genomes for the Human Microbiome Project
            and the work was funded by the National Institutes of Health (NIH)
            grant U54 HG004968.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..15861
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..15861
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..15861
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:10861](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..10861)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(670..1119)
                     /locus_tag="HMPREF2804_RS02655"
                     /old_locus_tag="HMPREF2804_02660"
     CDS             complement(670..1119)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397881.1"
                     /locus_tag="HMPREF2804_RS02655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02660"
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="WP_070448826.1"
                     /transl_table=11
                     /translation="MTAERTADLSARIGEPLGETPWMRISQQNINRFGELTGDTQWIHT
                     DPYRAAHTPFGGTIVHGAYLLSLAITFAGQLLPAEGTSMLVNAGVSAARFRSAVPVDSQ
                     VCGSASIVSAEDFGDATLAEIRVSVHVKGQSKPATTATVRMVLHD"
     gene            complement(1116..1769)
                     /locus_tag="HMPREF2804_RS02660"
                     /old_locus_tag="HMPREF2804_02665"
     CDS             complement(1116..1769)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005616.1"
                     /locus_tag="HMPREF2804_RS02660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02665"
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="WP_070627156.1"
                     /transl_table=11
                     /translation="MTDTPHASSAPSPEPLPPEGSDASGYPFTFYLPDLGEGLRSGEII
                     EWLISEGQQVELDQVLVVVETAKSTVEIPSPVAGVVVRLLEPVGQVVDVDAPLIELRTP
                     TPQAQAPTAGADAPAKDSADKLPAKPGAGESKHLVGRGKMPIKPGGTGTALPPKSAKTQ
                     GGKARSGAKVSPAVRRLARSLGVDLKTVTGTGAGGAITPEDVQTAADHAAGGAS"
     gene            complement(1781..2860)
                     /locus_tag="HMPREF2804_RS02665"
                     /old_locus_tag="HMPREF2804_02670"
     CDS             complement(1781..2860)
                     /EC_number="1.2.4.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397883.1"
                     /locus_tag="HMPREF2804_RS02665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02670"
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="WP_083308330.1"
                     /transl_table=11
                     /translation="MSQQAADTQVRGAKTASDTAPQAPESTQSRSDAVQRTSMVGALNL
                     ALGHEMQHNDRVVMFGVDVGTLGGVFRVTNGLTERFGEERCFDTPISEAGIMGAAIGMA
                     MYGFRPVIEMQFDAFGYPAFEQMVSNLAKMRNRTRGDLSMPVVVRMPYGGGVGAVEHHS
                     DSSEGYAAHTPGLHVYTPSNPSDAYHMLRQAIRSDDPVIFYEPKRLYWEEGDLDTSANP
                     LPIGQARICRPGEDVTLISYGPTVPMALAAAETAAEYGYSVEVIDLRTLTPFDEHTVCE
                     SVMRTGRAVMVHEAPQTGGFGAEVVARITSRCFDYLERPVERVTGLDVPYPPPGLEHLY
                     LPNEEKILGTIATLYAEND"
     gene            complement(2857..3903)
                     /locus_tag="HMPREF2804_RS02670"
                     /old_locus_tag="HMPREF2804_02675"
     CDS             complement(2857..3903)
                     /GO_function="GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397884.1"
                     /locus_tag="HMPREF2804_RS02670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02675"
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="WP_070627158.1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 6.2e-09, bitscore:
                     28.5, seeds: 323, tool: rule-based-clusters)"
                     /sec_met_domain="DXP_synthase_N (E-value: 2.7e-09,
                     bitscore: 29.2, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNTLQSPPQQVQNGVSDDLLITAHAAILAGRHFNQQASNLAKQG
                     YMGAYPSSLGQEACQVAAALALEPADWLFPTYRDSVAVMSRGIDLTEVLSPFHGSSHCG
                     YAPTKTHTAPEATPLATHTAHAVGLAMAAKFAGDSTVALAMCGDGSTSEGDFHEALNLA
                     AVFEAPVVFLVQNNGYAISTPVSRQMRAASIAAKGEGYGMPAYRVDGNDFEQVYPRLHE
                     AVERARAGGGPTLIEAMTYRMGPHTTSDNPDRYRSREEVEQWRGRDPLTRIQHQMYQRL
                     PNAKTHIENAILAAEKIASEARTSMMQLQMPDPQDLFAHVYANPPATLSAVAEEYAQQH
                     AAQNGENA"
     gene            complement(4029..4982)
                     /locus_tag="HMPREF2804_RS02675"
                     /old_locus_tag="HMPREF2804_02680"
     CDS             complement(4029..4982)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397885.1"
                     /locus_tag="HMPREF2804_RS02675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02680"
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="WP_004005617.1"
                     /sec_met_domain="fabH (E-value: 3.4e-91, bitscore: 298.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVTISGVGAAVPNDPIPNSEFAALGVDDEWIASRTGVRQRHHLR
                     QEQTLAELALEASEKALADAGISAEEIDFVLAATTTADQVSPGLAPVVAAGLGAPQVGA
                     VDLNAACSGFLYALDYAASKIEQGSIDRALVIGADAMSRLTNPEDAGTAVLFADGAGAA
                     IVQAAPEVSEDEYPYMCSFGSDGSQAQALYISDSTQQVVMDGAEVYMAAVDAMSEEITY
                     VLDSCGIDPERIDQLVCHQANGRIMAAVARRLRWDSSKVLSYIDRYGNTSAASIPITLA
                     VGQQEGHVKPGDLLALTAFGAGFTWGAGVIGFKTTA"
     gene            complement(5001..6182)
                     /locus_tag="HMPREF2804_RS02680"
                     /old_locus_tag="HMPREF2804_02685"
     CDS             complement(5001..6182)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005618.1"
                     /locus_tag="HMPREF2804_RS02680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02685"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_070627160.1"
                     /sec_met_domain="AfsA (E-value: 3.4e-08, bitscore: 26.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTRQPPEDAPLAAGRASADPRYAAYLGCAYHPAPSTAPDADLTH
                     AEKPSAEDPANGGEYRVFLRYDTRIAPDVSGPLKLSVTDVEYLQDLHRQGIEALGREAL
                     GVKSADHFVLCGAGLTFTPHVLGGDVRGPDTTGADEPIELSLHVPVSTIRRRRGQPCYA
                     ISGAITLWVGDTCLAKSDGAALFLTADTFEELRGELGNTAEIQDAASAGGASAGLPKAV
                     RVWGTPYGHVQAAPARLCGVSDASQVLIGDVRHDDTAHGTETAQEQVEEPREKTSERLS
                     QNPPGTPALAQCRIVPTAPPRHFDRPLDHYPGLMMASAARQFAVAALAHRTGEDPANLR
                     VAEETHEFFEFVELTEIPTLNLLRCEKHRDTYQTEITVNQGGAVKARSRFTFI"
     gene            complement(6179..7420)
                     /locus_tag="HMPREF2804_RS02685"
                     /old_locus_tag="HMPREF2804_02690"
     CDS             complement(6179..7420)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (49-411). E-value:
                     3.6e-63. Score: 205.8. Matches aSDomain:
                     nrpspksdomains_HMPREF2804_RS02685_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397887.1"
                     /locus_tag="HMPREF2804_RS02685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02690"
                     /product="beta-ketoacyl synthase"
                     /protein_id="WP_070627162.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 9.5e-48, bitscore:
                     155.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="t2fas (E-value: 2.8e-123, bitscore: 404.3,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTRVVITGIGAVTPLGVGAETLHERAVAGLSGLNDENENGLGGK
                     VGRCLDFDVKNPLSRREMRRMDRYTQLAVAAADEAIAQAGWGGGLPCDARRITCYIGTA
                     IGGLDTLETQHLVMHAEGAEEVSPLTVPMMMANAAPAHISMRHGLHGETTAIVTACSSG
                     AQAIIAGVRSILLGEADAAVVGGAEAGTTRFTRAIFNAAGAISPDGNSTPFDEERNGFN
                     LGEGAGILVLESAENARARGAEILGEIAGYGLSSDAHHITAPNPDGMYAAYALNRALET
                     AGVTPEQVSYVNAHGTGTELNDTTEVNVLRQALGDALEDIPVSSTKSAIGHLFGAAGAV
                     ETIATLQALRHGQAPPTLGLKNLDKRLGQVRISGEPQDLAPGTFSAADGTPKRLGLSNS
                     LGFGGHNVTLAIRA"
     aSDomain        complement(6188..7273)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: False"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HMPREF2804_RS02685_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="3.60E-63"
                     /label="HMPREF2804_RS02685_PKS_KS.1"
                     /locus_tag="HMPREF2804_RS02685"
                     /protein_end="411"
                     /protein_start="49"
                     /score="205.8"
                     /tool="antismash"
                     /translation="LDFDVKNPLSRREMRRMDRYTQLAVAAADEAIAQAGWGGGLPCDA
                     RRITCYIGTAIGGLDTLETQHLVMHAEGAEEVSPLTVPMMMANAAPAHISMRHGLHGET
                     TAIVTACSSGAQAIIAGVRSILLGEADAAVVGGAEAGTTRFTRAIFNAAGAISPDGNST
                     PFDEERNGFNLGEGAGILVLESAENARARGAEILGEIAGYGLSSDAHHITAPNPDGMYA
                     AYALNRALETAGVTPEQVSYVNAHGTGTELNDTTEVNVLRQALGDALEDIPVSSTKSAI
                     GHLFGAAGAVETIATLQALRHGQAPPTLGLKNLDKRLGQVRISGEPQDLAPGTFSAADG
                     TPKRLGLSNSLGFGGHNVTLAI"
     CDS_motif       complement(6503..6550)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HMPREF2804_RS02685_0001"
                     /evalue="3.40E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="HMPREF2804_RS02685"
                     /protein_end="306"
                     /protein_start="290"
                     /score="10.1"
                     /tool="antismash"
                     /translation="YVNAHGTGTELNDTTE"
     gene            complement(7417..7674)
                     /locus_tag="HMPREF2804_RS02690"
                     /old_locus_tag="HMPREF2804_02695"
     CDS             complement(7417..7674)
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (21-76). E-value: 3.4e-12.
                     Score: 38.5. Matches aSDomain:
                     nrpspksdomains_HMPREF2804_RS02690_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005620.1"
                     /locus_tag="HMPREF2804_RS02690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02695"
                     /product="acyl carrier protein"
                     /protein_id="WP_070627164.1"
                     /sec_met_domain="PP-binding (E-value: 5e-11, bitscore:
                     35.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTETPVNTASLEEFVTQQVIDMGVPASTVKLDAKIDSMGLDSLDV
                     VELTQAVKKQLMIPVKPKDFENASTLQDAVDIIREKAQEK"
     aSDomain        complement(7447..7611)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HMPREF2804_RS02690_PP-binding.1"
                     /evalue="3.40E-12"
                     /label="HMPREF2804_RS02690_PP-binding.1"
                     /locus_tag="HMPREF2804_RS02690"
                     /protein_end="76"
                     /protein_start="21"
                     /score="38.5"
                     /tool="antismash"
                     /translation="MGVPASTVKLDAKIDSMGLDSLDVVELTQAVKKQLMIPVKPKDFE
                     NASTLQDAVD"
     gene            complement(7789..8553)
                     /locus_tag="HMPREF2804_RS02695"
                     /old_locus_tag="HMPREF2804_02700"
     CDS             complement(7789..8553)
                     /NRPS_PKS="Domain: PKS_KR (8-161). E-value: 4.7e-08. Score:
                     25.1. Matches aSDomain:
                     nrpspksdomains_HMPREF2804_RS02695_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397888.1"
                     /locus_tag="HMPREF2804_RS02695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02700"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_048754943.1"
                     /sec_met_domain="adh_short (E-value: 1.9e-32, bitscore:
                     105.4, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.8e-34, bitscore:
                     112.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHQKRPGCALVTGASGDIGAQIALRLAQDGWNIAVGYATGIERA
                     EEVAGRIRECGVSAMPIKIDVTSTTSVDDAFDALEDSLGQVTVLVNNAGIRSDGLVAGL
                     SDDEWDAAISTNLSSVFRTSRRALGPMIRARFGRIINISSILAGRTIAGTGSYSAAKSG
                     ILGITRAMAIEVARRGVTVNAVCPGLVETSMTEEVETFTQSVQRAVPMARPAQARDIAE
                     CVAFLASDRADYITGQSIAVDGGLSAQAFSLN"
     aSDomain        complement(8071..8529)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HMPREF2804_RS02695_PKS_KR.1"
                     /evalue="4.70E-08"
                     /label="HMPREF2804_RS02695_PKS_KR.1"
                     /locus_tag="HMPREF2804_RS02695"
                     /protein_end="161"
                     /protein_start="8"
                     /score="25.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="CALVTGASGDIGAQIALRLAQDGWNIAVGYATGIERAEEVAGRIR
                     ECGVSAMPIKIDVTSTTSVDDAFDALEDSLGQVTVLVNNAGIRSDGLVAGLSDDEWDAA
                     ISTNLSSVFRTSRRALGPMIRARFGRIINISSILAGRTIAGTGSYSAAK"
     CDS_motif       complement(8464..8523)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HMPREF2804_RS02695_0001"
                     /evalue="4.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="HMPREF2804_RS02695"
                     /protein_end="30"
                     /protein_start="10"
                     /score="12.9"
                     /tool="antismash"
                     /translation="LVTGASGDIGAQIALRLAQD"
     gene            complement(8678..9178)
                     /locus_tag="HMPREF2804_RS02700"
                     /old_locus_tag="HMPREF2804_02705"
     CDS             complement(8678..9178)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397889.1"
                     /locus_tag="HMPREF2804_RS02700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02705"
                     /product="ester cyclase"
                     /protein_id="WP_070627166.1"
                     /sec_met_domain="PF07366 (E-value: 1.3e-30, bitscore: 98.5,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKWGNIIEDMMAQMPEDLRTVALGVAEIFTNMDIETARKYIHPD
                     FVDHEASEGVGGGPEGYLATAKYMNQAFSDASWKPQKIVASADGQHYTMVIKFSGVHTG
                     EFMGVPATGKPFEIHHLHLFRVEDGKAIEHWGGRDELGLLRQIGVLNSEHPTPADAGQA
                     AFA"
     misc_feature    complement(9197..9212)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(9218..9886)
                     /locus_tag="HMPREF2804_RS02705"
                     /old_locus_tag="HMPREF2804_02710"
     CDS             complement(9218..9886)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397890.1"
                     /locus_tag="HMPREF2804_RS02705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02710"
                     /product="HAD family phosphatase"
                     /protein_id="WP_070448814.1"
                     /sec_met_domain="HAD_2 (E-value: 4.9e-12, bitscore: 38.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRTFMSPEYNTTKYVWTDFGGVLTPPVSHTMRVFCQKYGLTESQ
                     LRAGFEAVARNHNLTDPLELVDTPLMSEKEWLSEISEQVGGAFQLDTLADDWFRDREPN
                     ASWVAYLHEIKAHGVKVGLISNMVPTWDNHWRAMLNEPGLFDDIVLSFEVGYRKPQRGI
                     YDYAAELAGVNPAQCILIDDLARNCEGARAAGWDAIEFTTTEECVKQLHPLLSPAEETV
                     "
     gene            complement(9887..10861)
                     /locus_tag="HMPREF2804_RS02710"
                     /old_locus_tag="HMPREF2804_02715"
     CDS             complement(9887..10861)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397891.1"
                     /locus_tag="HMPREF2804_RS02710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02715"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_070627168.1"
                     /sec_met_domain="AfsA (E-value: 8.5e-17, bitscore: 53.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLVYESAPELDETITIDPDIENLHYDRTVDRHKVHRAAVSEVFL
                     TDIRRQSTQHVLVAAQLPSSHSFFHDSIYNDAEGTPDALLLLEIARQATIASAHEVGVD
                     VGNTLISNEFGLTIYPHEIAALNPEDTNLLIRNYFDWTSIRRGAPRSGRCYQQLIMGNR
                     VIAEHFSGGRILTKNQLQILRSEYRGTPPPTTANLHELTFTDVQDPIAPERVGRRNPLN
                     VVISQLNTAEAPSAQVTPNVANKALFDHAYDHITMQILTEAARQLYLATRPDSERAPEI
                     SSIRGNFLAFAELDSPVQIRATAPGEFVVEQDNRQIADFALKS"
     misc_feature    complement(11002..11017)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.66"
                     /tool="antismash"
     misc_feature    11010..11024
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.7"
                     /tool="antismash"
     gene            complement(11403..12812)
                     /locus_tag="HMPREF2804_RS02715"
                     /old_locus_tag="HMPREF2804_02720"
     CDS             complement(11403..12812)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005625.1"
                     /locus_tag="HMPREF2804_RS02715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02720"
                     /product="MFS transporter"
                     /protein_id="WP_070448809.1"
                     /transl_table=11
                     /translation="MSVTTPHPKRWAILGFMCLALVVTGLDSLIVAVAIPSMETGLQAT
                     GEQLQWVVAAYSLAFAAPLLFFGGLADRIGHKLSFLAGMFILLLGSILAAFSINAEVLI
                     FSRVIMGLGSAFIMPSTLALIRDIFPDHERAKALGIWVGMSSLGIPLGPIVGGLLLKSF
                     SWGSIFLINVPIIAVAFLACLMLAPESSKKHSSRLDFFGLILSIFGPALLVYGIIAAPE
                     AGWTSSMTLTLLIAGVVLSLIFIVWERRSASPMLSHEVFRNRRFGGPLLTISSVFFGVF
                     GGLFIMTQHLQFVLGLDPFTSGLHMLAMCSAVLVAPISPKLVEKFGLGPVSATGPLMVA
                     LGMVLLAISPSPKSGQVVVALFTFGLGVGFGAPASVNSIMESTPKKQTGAGSAVADVAM
                     QLGGALGIALMGSIGIATATAQSPRGVAAACWVGAAVGLLGAVTVFTVLPKTGAPAVER
                     AVAPEPAAKLP"
     misc_feature    12970..12986
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.08"
                     /tool="antismash"
     misc_feature    12972..12985
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.37"
                     /tool="antismash"
     misc_feature    complement(12975..12989)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.88"
                     /tool="antismash"
     misc_feature    13316..13333
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(13451..13918)
                     /locus_tag="HMPREF2804_RS02720"
                     /old_locus_tag="HMPREF2804_02725"
     CDS             complement(13451..13918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005626.1"
                     /locus_tag="HMPREF2804_RS02720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02725"
                     /product="lysozyme"
                     /protein_id="WP_070627170.1"
                     /transl_table=11
                     /translation="MHQISPEGIAFLKEKEGFRSDAYYDVAGVLTIGYGHSTRAPSIEQ
                     YPVYEGQHITEEEGEEILRADLKPTEAAVNSAVTREITQKQYDALVSFTFNVGTGAFKS
                     SDVLSYTNAGNYQAAADELLQYVYAGGRLIDGLRKRREEERAMYLSSLPQE"
     misc_feature    complement(13933..13949)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.83"
                     /tool="antismash"
     misc_feature    complement(13936..13951)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.18"
                     /tool="antismash"
     misc_feature    14060..14073
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.1"
                     /tool="antismash"
     gene            complement(14065..15861)
                     /locus_tag="HMPREF2804_RS02725"
                     /old_locus_tag="HMPREF2804_02730"
     CDS             complement(14065..15861)
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (68-497). E-value: 2e-109.
                     Score: 357.9. Matches aSDomain:
                     nrpspksdomains_HMPREF2804_RS02725_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397896.1"
                     /locus_tag="HMPREF2804_RS02725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2804_02730"
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="WP_083308334.1"
                     /sec_met_domain="AMP-binding (E-value: 3.8e-118, bitscore:
                     387.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDPQYHDSHPHDAQAPAQESQRPAPAQTTPDTASGQNPLAHRPW
                     LKNYSPGVAVDVHLPETSLSHLIDDAVREVPHKVALEFFGATTTYAELGSQIDRAAEGL
                     RLAGVQAGDRVALILPNCPQHIIAFYAVLRLGAIVVEHNPLYTGAELRHMFEDHGAKAA
                     IVWDKISGHITSLPADIKPAHIYAVNLIKAMPVFTRAALKLPVKKARESREKLEGAAPG
                     TTSWAQLLKRPPIDPDFKRPTAHDVALLQYTSGTTGLPKGVMLTHRNLESNGRMGEAWI
                     KPSDDECIYSVLPLFHAYGMTLGVTIAALCRARLVLFPTVDMGLITKAMKKTRPTILPA
                     VPPVYRRLMDVAKEQGISLQGVRYAVSGAMNLPPELVAEWEDASGGYMVEGYGLTECAP
                     LVSCNPLNDTRRAGSIGIPFPSTDIRVVDPETLQDVPLGEEGELWVHGPQCFAGYWKRE
                     EDTQKTITADGWLRTGDIVRLDEDYFIQIVDRIKEVIITGGFNVSPTEVETALKQHDSV
                     ADAAVVGIPQASGGEMVVAAVIPAEGHAVDEHVLREHCYARVTRYKVPRKIVAVDDLPR
                     SMLGKILRRKVREQLIASGEFD"
     aSDomain        complement(14371..15657)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HMPREF2804_RS02725_AMP-binding.1
                     "
                     /evalue="2.00E-109"
                     /label="HMPREF2804_RS02725_AMP-binding.1"
                     /locus_tag="HMPREF2804_RS02725"
                     /protein_end="497"
                     /protein_start="68"
                     /score="357.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="DDAVREVPHKVALEFFGATTTYAELGSQIDRAAEGLRLAGVQAGD
                     RVALILPNCPQHIIAFYAVLRLGAIVVEHNPLYTGAELRHMFEDHGAKAAIVWDKISGH
                     ITSLPADIKPAHIYAVNLIKAMPVFTRAALKLPVKKARESREKLEGAAPGTTSWAQLLK
                     RPPIDPDFKRPTAHDVALLQYTSGTTGLPKGVMLTHRNLESNGRMGEAWIKPSDDECIY
                     SVLPLFHAYGMTLGVTIAALCRARLVLFPTVDMGLITKAMKKTRPTILPAVPPVYRRLM
                     DVAKEQGISLQGVRYAVSGAMNLPPELVAEWEDASGGYMVEGYGLTECAPLVSCNPLND
                     TRRAGSIGIPFPSTDIRVVDPETLQDVPLGEEGELWVHGPQCFAGYWKREEDTQKTITA
                     DGWLRTGDIVRLDEDYFIQIVDRIKEVIIT"
     CDS_motif       complement(14476..14556)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HMPREF2804_RS02725_0002"
                     /evalue="4.70E-08"
                     /label="NRPS-A_a6"
                     /locus_tag="HMPREF2804_RS02725"
                     /protein_end="462"
                     /protein_start="435"
                     /score="25.4"
                     /tool="antismash"
                     /translation="PLGEEGELWVHGPQCFAGYWKREEDTQ"
     CDS_motif       complement(15061..15120)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HMPREF2804_RS02725_0001"
                     /evalue="4.40E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="HMPREF2804_RS02725"
                     /protein_end="267"
                     /protein_start="247"
                     /score="25.4"
                     /tool="antismash"
                     /translation="ALLQYTSGTTGLPKGVMLTH"
ORIGIN
        1 gccagcatag acttagcggc gcgcaccgta tgagaggcag aaccggcaat ctgctccgcg
       61 agctgttcgg cgcatgcgtt caactcaccc ggctccgaca cccgatgcac cagcgaaagc
      121 tcctgcgcac gcgcggcacg cagaacaggc tgggtgtata acagctcggt ggcggcgctg
      181 aacccggcgt agcgcggtaa gaaccagctc atcccgccgt ccgtggaggc cccgatcatg
      241 ccgtaggcga tattgagttt ggtgcgcggc tcggcaacac gcagatcgca tgcgagcgct
      301 aaggacatgc ccgcccccgc cgcctgcccg ttaatggcgg cgataaaggg gatgggtgcg
      361 gcaacgatcc ctagaataat atcttcgagc agacgcgcga gttcgcggct gtacgctaag
      421 gattcttctt tttcgcgcga cataccttcg gcgaagtcgt cgtagctgcc gcctagacta
      481 aaatcctgcc cggccccggt gagcacggca gctttgagcg tatcgtcctc atgcagccgg
      541 ttgagcacct cgcgcagctc ggtcgccagg tggcggctga gggtgttgcg gttttcgggc
      601 tgattcaggg tcacatacgc gatgccgccg cggcgttcga ggagtacggc ggggttatct
      661 gcggtggtgt tagtcatgaa gtaccatccg aactgtggct gttgtggcgg gtttgctctg
      721 gcctttaaca tgcacggaaa cacgaatttc ggcgagggtg gcatccccaa aatcttcagc
      781 gctcacgatt gatgcgctgc cgcatacctg cgagtccacc ggcacggcgc tgcggaacct
      841 ggcggcactc accccggcgt ttacgagcat ggaggtgccc tctgcgggga gcagctgccc
      901 ggcgaacgtg atggcgaggc tcagcagata cgccccgtgt acgatggtgc cgccgaaggg
      961 ggtgtgcgcc gcgcggtacg ggtctgtgtg aatccactgg gtgtcgccgg tgagctcgcc
     1021 gaagcggttg atgttctgct ggctgatgcg catccagggg gtctcgccca ggggttcgcc
     1081 gatgcgcgcg ctcaggtcgg cggtgcgttc cgcagtcatg atgcgcctcc ggcggcgtga
     1141 tcggcggcgg tttgtacgtc ttcgggggtg atagcgccgc ctgcgccggt tccggtgacg
     1201 gttttcagat cgaccccgag ggagcgggcg agcctgcgca cggcgggcga aactttggca
     1261 cccgaacgag ccttgccgcc ttgagttttg gcggattttg gcggcagcgc ggtaccggtg
     1321 ccgccgggtt tgatgggcat tttgcccctg cccaccaggt gtttgctctc tcccgcgccg
     1381 ggtttggcgg ggagcttatc ggctgaatcc ttcgcggggg cgtctgcacc agccgtgggc
     1441 gcctgggcct gcggggtggg ggtgcgcagc tcgatgaggg gcgcatccac atcgacaacc
     1501 tgaccgaccg gttcgagcag acgcaccacg acgcccgcga ccggcgaggg gatttcgacc
     1561 gtggatttgg cggtttcaac caccacgagc acctgatcca gctcaacctg ctgcccttcg
     1621 ctgatgagcc actcgataat ctcacccgag cgaagcccct cccccaaatc cggcagataa
     1681 aaggtgaagg ggtagccgga agcgtcggaa ccctcgggcg gtaagggttc ggggcttggg
     1741 gcggatgaag cgtgcggtgt atcagtcatg cctgtttctc ctagtcgttc tcggcgtaga
     1801 gggttgcgat agtacccaga atcttctctt cgttcggcaa atacaggtgt tccagcccgg
     1861 gcggcggata gggcacgtcc aggccggtca cacgctctac ggggcgttct aagtagtcga
     1921 aacagcgtga ggtaatgcgc gccaccacct cggcaccaaa tccgccggtc tgcggggcct
     1981 cgtgtaccat gacggcgcgg ccggtgcgca tcacgctttc gcatacggtg tgttcgtcga
     2041 acggggtgag ggtgcgcaga tcgatcactt caacgctgta cccgtattcg gcggcggttt
     2101 cggcggcggc aagcgccatc ggtaccgtcg gcccgtagct gataagggtt acgtcctcgc
     2161 cggggcggca aattcgtgcc tgcccaatcg gcagagggtt cgccgaggta tcaaggtcac
     2221 cctcttccca gtacagacgc ttgggttcgt agaaaatcac cgggtcgtcg ctgcgaatcg
     2281 cctggcgcag catatggtag gcatccgaag ggttggaggg ggtgtacacg tgcagcccgg
     2341 gagtatgcgc ggcgtacccc tccgagctgt cgctgtgatg ctccacggca cccaccccgc
     2401 cgccgtaggg catgcgcacc accacgggca tggagaggtc gccgcgcgtg cggttgcgca
     2461 ttttcgccag attgctcacc atctgttcaa aggcggggta gccgaaagcg tcaaactgca
     2521 tttcgatcac ggggcggaac ccgtacatcg ccatgccgat cgcggctccc ataattcccg
     2581 cctcagaaat gggggtgtcg aagcagcgtt cctcaccgaa ccgttcggtc agcccgttgg
     2641 tcacacggaa aacaccgccg agggtgccta cgtccacgcc gaacatcacc acgcggtcgt
     2701 tatgctgcat ttcgtggccc aaggccaggt tgagggcgcc caccattgag gttcgctgca
     2761 ccgcatccga gcgggattgg gtcgattccg gtgcctgagg tgcggtgtcg gatgcggtct
     2821 tcgcgccgcg tacctgagta tctgctgcct gctggctcat gcgttttctc cattctgcgc
     2881 ggcgtgctgt tgtgcgtact cttcggcgac agcgctcagg gttgccggcg ggttcgcata
     2941 cacgtgggcg aaaaggtcct gtgggtcggg catctgcagc tgcatcatgc tggtgcgggc
     3001 ctcggaggcg atcttttctg cggctaagat cgcgttttcg atatgggtct tcgcgttcgg
     3061 caggcgctgg tacatctgat gctgaatgcg ggtgagcggg tcacgcccgc gccactgctc
     3121 gacttcttcg cggctgcggt accgatcggg gttgtcggag gtcgtatgcg gccccatgcg
     3181 gtaggtcatc gcctcaatca gggtgggtcc gccaccggca cgggcgcgct cgacggcttc
     3241 gtgcaggcgg gggtacacct gctcaaagtc attgccgtca acccgatatg cgggcatgcc
     3301 gtacccttca cccttcgcgg cgatacttgc cgcgcgcatc tgccgggata cgggggttga
     3361 gatagcgtac ccgttattct gcacgaggaa aacgacgggc gcctcgaaga cagcggcgag
     3421 gttaagcgcc tcatgaaagt cgccttcgct tgtggaaccg tcgccgcaca tcgcgagagc
     3481 taccgtggaa tcaccggcga actttgccgc cattgccaga ccgaccgcgt gggcggtatg
     3541 cgtggcgagc ggtgttgcct ccggggcggt atgcgttttc gtgggggcat acccgcagtg
     3601 tgaagacccg tggaagggtg agagaacttc ggtgaggtcg ataccccggc tcatgacggc
     3661 gaccgaatcg cggtaggtag gaaaaagcca gtcggcgggt tctaaggcga gggcggcggc
     3721 aacctggcac gcctcctgcc ccagggatga ggggtacgcc cccatatatc cctgtttggc
     3781 gaggttgctc gcctgctgat taaagtgcct gcccgcgagt atggcggcgt gggcggtgat
     3841 gagcaggtca tcgctcacac cgttctgcac ctgctgcggc ggggattgta gtgtgttggg
     3901 catgttaacg cctatctgtc ggtcccctca taaaagctct tttgatgagg ggtgtgggtg
     3961 gcataagagt gaaatacggt ggcggattct agctgcgcgc aaccgggatg cgccctggcc
     4021 gccagcgctc aggcggtggt tttgaatccg atcacgcccg cgccccaggt gaatcccgcc
     4081 ccgaaggcgg tcagcgccag caagtctccc ggtttcacgt gcccctcctg ctgcccgacg
     4141 gctagggtaa tcgggatgga ggcggcagag gtgttcccgt accggtcgat atatgagagc
     4201 actttggagg aatcccagcg cagccgccgt gcgaccgccg ccataatcct tccgttggcc
     4261 tggtggcaga ccagctggtc gatcctctcc gggtcgatac cgcacgagtc gagcacgtag
     4321 gtgatttcct cggacatcgc atccaccgcc gccatataga cctcggcgcc gtccatcacc
     4381 acctgctgcg tggagtcaga aatatacagt gcctgcgcct ggctgccgtc ggaaccgaac
     4441 gaacacatat aggggtattc gtcctcgctc acctcggggg ccgcctgcac aatggcggca
     4501 ccggcaccgt cggcgaagag taccgccgtg cccgcatcct cggggttagt caggcgtgac
     4561 atcgcatcgg caccaatgac gagcgcccgg tcgatactgc cctgctcgat ttttgaggcg
     4621 gcgtagtcga gggcgtacag gaacccggaa catgcggcgt tcagatcgac cgcgcccacc
     4681 tgcggggcgc ctaaacctgc ggcgacgacg ggcgcgagcc ccggcgaaac ctggtcagcg
     4741 gttgtggttg cggcaagcac aaagtcgatt tcttcggcgc ttataccggc atccgcaagt
     4801 gccttctcgc tcgcttcaag cgccagctcg gcgagagtct gctcctggcg caggtggtgg
     4861 cgctggcgca ctccggtgcg actggcaatc cattcgtcgt ccacgccgag cgcggcgaat
     4921 tcgctgtttg gaatggggtc attgggcacg gcggcgccaa cgcccgaaat tgtcacactc
     4981 atggtgcctt tcttgaattt ctagataaag gtgaatcggc tgcgtgcctt aacggcaccg
     5041 ccctgattca cggtgatctc tgtctgatag gtgtcgcggt gtttctcgca ccgcagcaga
     5101 ttgagggttg gaatctcggt gagttccacg aactcgaaaa attcgtgggt ttcttccgcc
     5161 acgcgcaggt tcgcggggtc ttccccggta cgatgcgcga gcgccgccac cgcgaactgc
     5221 cgggctgcgc ttgccatcat gagccccgga tagtggtcca gcgggcgatc gaaatgccgt
     5281 ggcggtgctg tgggcacgat gcggcattgg gctagtgcgg gggttcccgg cgggttctga
     5341 gagagccgtt ccgaggtttt ctcccgcggc tcttccacct gttcctgggc ggtttcggta
     5401 ccgtgagccg tatcgtcatg acgcacgtct ccgatcaaaa cctgggatgc gtcgctcacc
     5461 ccgcacaggc gcgcgggggc agcctgcacg tgcccgtacg gcgttcccca cacacgcaca
     5521 gccttcggga gtccggcgga tgcgccaccg gcggaagccg catcctggat ttctgcggtg
     5581 ttcccgagtt caccgcgcag ttcctcgaag gtatcggcgg tgaggaagag cgccgcgccg
     5641 tcgcttttag ccaggcaggt atcgcccacc cagagggtga tcgccccgct gatcgcatag
     5701 cacggctgcc cgcgcctgcg gcggatggtg ctcacgggaa cgtgcaggct cagctcgatc
     5761 ggctcgtctg cgccggttgt gtcggggccg cgaacgtcgc cgcccagaac atgcggggtg
     5821 aaggtgagcc ctgcgccgca aagcacgaaa tggtcggcgg atttcacgcc gagcgcttcg
     5881 cgcccgagcg cctcgatccc ctggcggtgc aggtcttgca ggtattccac atcggtcacg
     5941 ctcagcttga gtggcccgct cacatcgggg gcgatgcgcg tatcgtaccg caggaacacg
     6001 cggtattccc cgccattcgc cgggtcttca gcggagggtt tttccgcgtg cgtgaggtcc
     6061 gcatcgggtg ctgtgctggg ggcaggatgg taggcgcatc cgagatacgc cgcatatcgc
     6121 gggtctgcgg atgcacgtcc cgccgccagt ggcgcatcct cgggaggctg ccgagtactc
     6181 atgcgcgtat cgccagggtc acgttgtgcc cgccgaagcc cagggagttt gagagtccca
     6241 gccttttggg tgtgccgtcg gccgcgctga acgtgccggg tgctaaatcc tgcggttcgc
     6301 cgctgatgcg cacctgcccc agtcgcttat ccaggttttt cagccccagg gtgggcggtg
     6361 cctgcccgtg ccgaagcgcc tgaagcgtgg cgatggtctc gacggcgccc gcagccccga
     6421 agaggtgccc aatcgccgat ttcgtggagg aaaccgggat gtcctcaagg gcatccccga
     6481 gcgcctgccg cagcacgttc acttcggtcg tatcgttcag ctcggttccg gtgccgtggg
     6541 cgttcacgta cgatacctgt tcgggggtta cccccgcggt ttcaagcgcg cggttgagcg
     6601 cataggccgc atacatgccg tccgggttcg gcgccgtaat gtggtgagcg tctgaggaga
     6661 gcccgtaccc tgcgatttcg cccagtatct cggcgcctcg ggcgcgggcg ttctcggctg
     6721 attcgagcac gagaataccg gcaccctccc ccaggttgaa gccgttgcgc tcctcatcga
     6781 agggggtcga gttcccgtcg ggcgagatcg ccccggcggc gttgaagata gcgcgggtga
     6841 accgggtggt tcccgcctct gcgccgccta cgacggccgc atccgcctcg cccagcagaa
     6901 tactgcgcac cccggcgatg atcgcctgcg cccccgagga gcacgccgtg acgattgcgg
     6961 ttgtttcgcc gtgcagcccg tggcgcatcg aaatatgcgc gggcgcggcg ttcgccatca
     7021 tcatgggcac ggtcagcggc gagacttcct cggcgccctc ggcgtgcatc accaaatgct
     7081 gcgtttcgag ggtgtccagc ccgccgatag cggtaccaat atagcaggtg atgcgccgcg
     7141 catcgcacgg cagcccgccg ccccagcccg cctgcgcgat agcctcatca gccgccgcca
     7201 cggcaagctg ggtgtaccgg tccatgcggc gcatctcgcg gcgactgagc gggttcttca
     7261 cgtcaaagtc gaggcatcgc ccaaccttgc cgcccagccc gttctcgttt tcgtcgttca
     7321 gcccgctcag gcccgccacg gcacgctcat gcagcgtctc ggcgccaaca cccagcgggg
     7381 tgacggcacc gattcccgtg atgactactc tcgtactcac ttctcctgtg ccttttcacg
     7441 aataatatct acggcatcct gcagggtgga ggcgttttca aaatccttgg gcttgacggg
     7501 aatcatcagc tgcttcttga ccgcctgggt gagctcaaca acatccaggg agtcgagtcc
     7561 catcgaatcg attttggcgt cgagcttgac ggtgctggcg ggcacgccca tgtcgatcac
     7621 ctgctgggtc acgaactctt ccagggatgc ggtgttaacg ggggtctcag tcatggttct
     7681 ctcctcatgg attatctggt gttttctgtg gttggttatc ttgtgttcac ggcgggtacc
     7741 cgtgaactca tccggcaaac ggcgggctta ctcgtagcga gcgcgaacct agttcagtga
     7801 aaacgcctga gcgctcagcc caccatctac cgcaatgctc tgcccggtaa tgtagtcggc
     7861 gcggtcgctg gcaaggaacg ccacgcactc ggcaatatcg cgtgcctgcg cggggcgagc
     7921 catcggcacg gcgcgctgca ccgactgggt gaaggtttca acctcttcgg tcatggaggt
     7981 ttcaacgagt ccggggcata ccgcgttcac ggtcaccccg cgtcgcgcca cctcgatagc
     8041 catcgcgcgg gttatcccca aaattccgct tttcgcggcg gaatagctgc cggttccggc
     8101 gattgtcctc ccggcgagaa tcgaggaaat attgatgatt cgcccaaaac gtgcgcgaat
     8161 catcggcccc aacgcccgcc gagaggtacg aaaaaccgag cttaagtttg tcgaaatcgc
     8221 cgcatcccat tcgtcgtcgc tgagccccgc aaccagcccg tcggaacgaa ttccggcgtt
     8281 attgactagc acggtgacct gccctaacga gtcttctagt gcatcaaagg cgtcatcaac
     8341 tgaggtggtg ctggtgacgt caattttgat gggcatcgct gacacgccgc actcgcggat
     8401 gcgcccggca acctcttcgg cgcgctcgat tccggtggcg tagccgacgg ctatattcca
     8461 tccgtcctgc gccagtctga gcgcgatttg tgcgccaata tctcccgacg ctccggtgac
     8521 gagggcgcat cctgggcgct tctggtgtgt catctgactc cttaggtttg gttcttatgc
     8581 ggcggcgggt tcacggcgta tcatatgccg tggtagttcg ccgcgcggtg ctcaatagcg
     8641 ttacgtggga tgcgtgaatg cgtgtttcgc ccgtgtttta ggcgaaggcg gcctgcccgg
     8701 cgtctgcggg ggtggggtgc tcggagttga gaacaccgat ctggcgcagc aggccgagct
     8761 catcgcgccc gccccagtgc tcgattgcct tgccgtcttc aacacggaaa aggtgcaggt
     8821 ggtgaatctc gaagggcttg ccggtggcgg gaacccccat gaactcgccg gtatgaacgc
     8881 cgctgaactt gatcaccatg gtgtagtgct gaccgtccgc gcttgccaca attttctggg
     8941 gcttccacga ggcatcggag aacgcctggt tcatgtattt cgcggttgcc aggtaccctt
     9001 cggggccgcc gccaacgcct tcgctcgcct catgatcgac gaagtcgggg tgaatgtact
     9061 tgcgggcggt ttcgatatcc atgttcgtga aaatctcagc cacgccgagg gcaacggtgc
     9121 gcaggtcttc gggcatctgc gccatcatgt cttcgataat atttccccac ttacccatgg
     9181 gttttccttt cgttgggtga ccttccggtc acggggttta aactgtttcc tctgccggtg
     9241 agagcagggg gtgcagctgc ttcacgcact cttcggttgt ggtgaattcg atggcatccc
     9301 acccggctgc acgggcgcct tcgcagttac gcgcaaggtc gtcaatgagg atgcactgcg
     9361 ccgggttcac tccggcaagt tctgcggcgt agtcgtagat accgcgctgc ggtttgcggt
     9421 atccgacctc gaaggagagc acaatatcgt cgaaaagacc gggttcgttg agcatggcgc
     9481 gccagtggtt atcccaggtg ggcaccatat ttgagatcag ccctaccttc acaccgtgcg
     9541 ccttgatctc gtgcaggtac gccacccagg atgcgttggg ctcgcggtcg cggaaccagt
     9601 cgtcggcgag ggtgtcgagc tgaaatgccc cgccgacctg ctcggaaatt tcgctcagcc
     9661 actctttttc gctcatcagg ggcgtatcga cgagttccag cgggtcggtg aggttgtggt
     9721 tgcgggcgac cgcctcaaac cccgcgcgca gctgagactc ggtgagcccg tatttctggc
     9781 agaagacgcg catcgtgtgg ctgaccggcg gggtgagtac tccgccgaag tccgtccaca
     9841 catatttcgt ggtgttgtat tcgggcgaca taaaggtcct tgtcatctag cttttgagcg
     9901 cgaaatccgc aatctgccgg ttatcttgtt caaccacgaa ttccccgggt gccgttgcac
     9961 ggatctgcac gggtgaatcg agttctgcaa acgccaggaa gttcccgcgt attgaggaaa
    10021 tttcgggggc gcgctcgcta tccgggcggg ttgccagata gagctgccgt gccgcctcgg
    10081 tcaaaatttg catggtgatg tggtcgtagg cgtggtcgaa aagcgcctta ttcgccacgt
    10141 tcggggtcac ctgcgcactc ggggcttcgg cagtgttcag ctgcgagatc accacgttga
    10201 ggggattgcg cctgcccaca cgctcggggg cgatgggatc ctgcacgtct gtgaaggtca
    10261 gctcgtgcag gttcgcggtc gtgggcggtg gggtgccgcg gtattcgctg cgcagaatct
    10321 gcagctggtt tttggtgagg atgcgcccgc cgctgaagtg ctcggcgatc acccggttgc
    10381 ccatgatgag ctgctggtag caccgaccgg aacgcggggc accgcgccga atggaggtcc
    10441 agtcgaagta gttgcgaatc agcaggttgg tgtcttcggg gtttagcgcg gcgatttcgt
    10501 gcggataaat cgtgagccca aactcgttgg agatcagggt gtttccgacg tctacaccga
    10561 cttcgtgggc gctggcgatt gtcgcctgcc gcgcaatttc gagcagcagg agcgcatcgg
    10621 gggttccttc ggcatcgttg tagatgctgt cgtgaaagaa gctgtgcgat gagggcagct
    10681 gcgccgccac aagcacatgc tgcgtgctct gccggcgaat atccgtgagg aaaacttcgg
    10741 aaaccgctgc acggtgtacc ttatgacgat cgacggtacg gtcatagtgc agattttcta
    10801 tatccgggtc tatcgtgata gtttcgtcga gctctggtgc cgactcgtat acgagggtca
    10861 ttgccctcct cctttggctg tggttggtgt atgttgcacg tggttatggg gtcccgcggg
    10921 gcttactgcg tgcgggtaag taagctcctt gggtgtgcgt tatcggtgtt gttatgccct
    10981 gccgctggat acgggcaata tggtgaagat tctcaatatc aaaaagacat aagtatggtc
    11041 actctggtgt gaaggtgtga tgatcccttg atattcctgc ggattcacga tggatgcggg
    11101 ttgcgaaaga ccgaaagcgc agcggcttgt ctatcccggc gtatacctgg gagtacgccg
    11161 ctgcccgtga ggttgggtca gcgaggatct gaatgtcgga aggcttcgag tgctcaggca
    11221 gcgcaccgta tcgtgcgtta tctggcgtct gttatgaatt ctgctgattt cgtcggtgtt
    11281 catagcgttc attccttttt catccctacc gtgtgggtag gagataccat cggaacgttt
    11341 cggaagtttg agttaggttc tgatggttgt ctgtggttgg ttgaaaaata tgtgcagctg
    11401 gcctagggca acttagctgc gggttctggc gcgaccgctc gttcaacggc gggtgcgcca
    11461 gttttgggaa gcacggtgaa gacggtaaca gcacccaaaa gcccgactgc cgcacctacc
    11521 cagcacgcag cggctacgcc tcggggcgat tgtgccgttg cggtggcgat tccgatactt
    11581 cccatcagcg cgatacccag ggcgccgccg agctgcatag caacatcggc aaccgccgag
    11641 cccgcgccgg tttgtttctt gggggtcgat tccatgatcg agttcacgga ggccggggcg
    11701 ccaaaaccca cacccagacc gaacgtgaag agcgctacca caacctgccc gctcttgggc
    11761 gaggggctga tagccagaag caccatgccg agggcgacca tgagagggcc ggtagctgag
    11821 acgggaccca gcccaaactt ttcgacgagt ttgggcgaga tcggtgccac gagaaccgcc
    11881 gaacacatgg cgagcatatg cagaccggat gtaaaagggt caagccctag gacgaactgc
    11941 aaatgctgag tcatgatgaa cagcccgccg aagaccccga aaaataccga agagatcgtc
    12001 agcagcggcc cgccgaaccg gcggttacgg aagacctcat ggctgagcat gggcgatgcg
    12061 ctgcggcgct cccacacgat gaagatgagg ctcagcacca cacccgcaat cagcagggtc
    12121 agcgtcatgc ttgaggtcca gcctgcttcc ggggcggcga taatgccgta caccagcagg
    12181 gcgggcccga aaatcgagag gatcagcccg aagaagtcca ggcgggaaga gtgttttttt
    12241 gaggattccg gggcgagcat caggcatgct aagaatgcca cggcaataat aggcacgttg
    12301 atcaggaaaa tggatcccca cgaaaaactt ttgagcagca atccgcccac aatgggaccg
    12361 agcggaatac ccagcgagga cattcccacc caaattccga gagctttagc acgttcatgg
    12421 tctggaaaaa tatcacgaat aagtgctagg gtcgaaggca taataaatgc tgatccgaga
    12481 cccataatta ctcgtgaaaa aataagtact tctgcattaa tagagaaagc cgcaagtatt
    12541 gaaccaagca gcaaaataaa cataccggct agaaaactta gtttatgtcc gattctgtcg
    12601 gcaagaccgc caaagaaaag tagtggtgcg gcaaatgcaa gtgaataggc tgcaacaacc
    12661 cattgcaatt gttcgcccgt tgcctgtagt ccagtctcca tagacggtat agcgaccgcg
    12721 acaatgagcg agtccagccc ggtcacaacc agcgctaggc acataaaccc gaggatagcc
    12781 cagcgcttgg ggtggggtgt ggtcacactc attttttagt ccttgaagtg gttggaagac
    12841 atagctatat gtaacagtga tgtctcaagt atacagttca aattaggtct gtcaatcgtt
    12901 tattttctct atttacacgt caattattca cacatattta ttgccgccca agaaataaat
    12961 ttagtgtcgt tgtaaattat taaaagaatt gagagagtga aggtttagtt ttcatatttt
    13021 ccctgtaaat actttcatca aaattccccg gcaattatcg cataaacctc tcgtgctgtg
    13081 ttgagcgtat agcataatcc caccccattc accatggcac gatcaccatc tacagcgaag
    13141 gcgagcacac gatgagagcc ccttgtgtgt gatgcgttac gccccaaaat tttcggcgtg
    13201 tccgtctgct atgcaccacg aaacgaggga gaaacagacc gaaatctgtt cctccctcgc
    13261 tcgaatctat gggctcatag tgagtgtttc tggaacctcg tagtcttgta gagcgtatcc
    13321 gttagaccag attacgcact acctattctg ctgagtagcc agtcagggtc tccaccgatt
    13381 cggaagcttt agagcctttc ctgaagaacc tgtcaaggta ggcaaccccg acatcgtacc
    13441 gcactgaact ttattcttgg ggaagcgaag aaagatacat agccctttcc tcttccctgc
    13501 gcttgcgcag cccatctatg agtcgtccgc cagcatacac gtattgcagc agttcatctg
    13561 cggcagcctg ataatttccc gcgttcgtgt agctgagaac atcactcgac ttaaaggcac
    13621 ccgtacccac gttaaaggtg aatgacacga gcgcatcgta ttgcttctga gtaatttcac
    13681 gagtaacagc agaattcaca gcagcttcgg taggcttcaa atctgcgcga aggatctcct
    13741 ccccttcctc ttcggtgata tgctgcccct cataaacggg gtattgctca atactgggcg
    13801 cacgggtcga atgcccgtaa ccgattgtga gaacacccgc cacatcgtag taggcgtcac
    13861 tcctgaaccc ttctttctct ttcaagaagg caattccttc aggtgatatc tgatgcattg
    13921 gtatcaactc ctctgaagaa taattacgat ttcattaaga ggttccggtc cccaccgtaa
    13981 ctaaggattc agactcatac aagaggtata acagacagtc ttctgccttg acgctcgaac
    14041 tcgcagttgt gccagagtgt gtttttaatc aaactccccg ctggcgatta actgttcacg
    14101 taccttacgg cgcagaatct tgccgagcat cgaccggggc agatcgtcca ccgccacaat
    14161 cttgcgcggc accttataac gggtcacgcg cgcatagcag tgctcgcgca gcacatgttc
    14221 atcaaccgcg tgcccttcgg cgggaatcac ggcggcgacc accatctctc ccccgctggc
    14281 ctgcgggatg cccacgaccg ccgcatccgc cacggaatcg tgctgtttca gggcggtttc
    14341 cacttcggtg ggcgaaacgt tgaacccgcc ggtaatgatg acttccttaa tacggtccac
    14401 gatttgaatg aagtagtctt cgtccaggcg cacaatatcg ccggtgcgca gccagccgtc
    14461 agccgtaatg gttttctgcg tgtcttcttc gcgtttccag tacccggcga aacattgcgg
    14521 gccgtgaacc cacagctcgc cctcttcgcc gagcggcaca tcctgaagag tttcggggtc
    14581 taccacgcgg atatcggtcg aggggaaggg gataccgatt gacccggcgc ggcgcgtatc
    14641 gttgagcggg ttgcatgaga cgagcggcgc acattcggtc agcccgtacc cctcaaccat
    14701 gtacccgccg gatgcatcct cccactctgc caccagctcg ggcggcaggt tcatggcacc
    14761 cgaaaccgca tagcgaactc cctgcagcga gatgccctgc tctttggcga catccatcag
    14821 gcggcggtag acaggcggta ccgcgggcag aatcgtgggg cgggtcttct tcatcgcctt
    14881 agtaatcagg cccatatcga cggttgggaa gagcaccagc cgggcgcggc acagcgccgc
    14941 aatcgttacg cccagggtca tgccataggc gtggaagagc ggcagcaccg agtagatgca
    15001 ttcgtcgtcg ctgggtttaa tccaggcttc gcccatgcgc ccgttcgatt cgaggttgcg
    15061 gtgggtgagc atcacgccct tgggcagccc ggtggtgccg gaggtgtact gcaagagcgc
    15121 tacgtcgtgg gcggtgggcc gtttaaagtc ggggtcgatc ggcggcctct tgagcagctg
    15181 cgcccagctt gtggttccag gggcggcgcc ttcaagtttt tcgcgggatt cgcgcgcctt
    15241 tttgaccggc agtttgagcg ccgcgcgggt gaagactggc atcgctttaa taaggttcac
    15301 ggcgtaaata tgcgctggtt tgatgtctgc gggcaggctc gtaatatgcc cggaaatttt
    15361 atcccacacg atggcggcct tcgcgccgtg gtcctcaaac atgtgccgca gctcggcgcc
    15421 ggtgtagagg gggttgtgtt ccacgacgat cgcgcccagg cgcagaaccg cgtagaaggc
    15481 aataatatgc tgcgggcagt tgggcagaat cagggcgacc cgatcccccg cctgcacccc
    15541 ggccaggcgc agcccttcgg cggcgcggtc gatctgactg ccgagttcag cataggtggt
    15601 ggttgcgccg aagaattcga gggcgacctt gtggggaacc tcgcggacag catcgtcaat
    15661 caggtggctc agcgaggttt cgggcaggtg aacgtctacg gcgacgccgg gtgaataatt
    15721 tttgagccag gggcgatgcg ccagcgggtt ctgcccggag gcggtatcag gtgtagtttg
    15781 agcgggtgcg ggtctttgag attcctgcgc gggggcttgg gcatcgtgcg gatgcgagtc
    15841 gtgatattgc gggtcagtca t
//
