LOCUS       NZ_KV802698            10285 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptococcus sp. HMSC073F11 Scaffold367, whole genome shotgun
            sequence.
ACCESSION   NZ_KV802698
VERSION     NZ_KV802698.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptococcus sp. HMSC073F11
  ORGANISM  Streptococcus sp. HMSC073F11
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 547914)
  AUTHORS   Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R.,
            Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A.,
            Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) McDonnell Genome Institute, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 213x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001810785.1-RS_2024_11_05
            Annotation Date                   :: 11/05/2024 23:57:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,892
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 16 of 57
            Pseudo Genes (incomplete)         :: 34 of 57
            Pseudo Genes (internal stop)      :: 10 of 57
            Pseudo Genes (multiple problems)  :: 3 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:06:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 119608
            Orig. end                         :: 129893
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV802698.1.
            The WUSC is a large strain collection isolated from clinical
            samples. Each sample is associated with metadata, including source,
            isolation site, 16s rRNA, metabolic, and other phenotypic
            information. The goal is to sample an adequate number of important,
            yet minor species, further adding to the catelogue of sequenced
            bacterial genomes and improving the diversity of the genomes
            available to the public. WGS will be preformed on approximely 550
            isolates. Samples were selected based on RDP analysis at the genus
            level. This is a reference genomes for the Human Microbiome Project
            and the work was funded by the National Institutes of Health (NIH)
            grant U54 HG004968.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10285
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10285
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10285
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5285](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5285
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(606..1238)
                     /locus_tag="HMPREF2764_RS07335"
                     /old_locus_tag="HMPREF2764_07355"
     CDS             complement(606..1238)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /locus_tag="HMPREF2764_RS07335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07355"
                     /product="HAD family hydrolase"
                     /protein_id="WP_070528439.1"
                     /sec_met_domain="HAD_2 (E-value: 9.7e-39, bitscore: 128.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTHTFKELEVPSPDAKTIRG
                     FMGPPLESSFATCLPKELISEAVQIYRSYYKAKGIHEAQLFPQIVDLLKELSSKYPLYI
                     TTTKNTPTAQDMTKNFGIHHFFDGIYGSSPEAPHKADVIRQALQTHQLAPEQAIIIGDT
                     KFDMLGAQETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLKYFQ"
     gene            complement(1260..2132)
                     /gene="sdaAA"
                     /locus_tag="HMPREF2764_RS07340"
                     /old_locus_tag="HMPREF2764_07360"
     CDS             complement(1260..2132)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="HMPREF2764_RS07340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07360"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500050.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQAELDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAATPTGR
                     RLQKEIFGE"
     misc_feature    2116..2132
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            complement(2141..2812)
                     /gene="sdaAB"
                     /locus_tag="HMPREF2764_RS07345"
                     /old_locus_tag="HMPREF2764_07365"
     CDS             complement(2141..2812)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="HMPREF2764_RS07345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07365"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838909.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYDINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    2966..2982
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            3052..3702
                     /locus_tag="HMPREF2764_RS07350"
                     /old_locus_tag="HMPREF2764_07370"
     CDS             3052..3702
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="HMPREF2764_RS07350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07370"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_020902660.1"
                     /transl_table=11
                     /translation="MKSTTKKIKTTLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHMIYVGQELVIDGPVAPAATPAPTTYAAPAAQDETVSA
                     PAAETTEVAEEAAPVASTPAAEETVATAETSAPAATVSGSEAEAKEWIAQKESGGSYTA
                     TNGQYIGRYQLTDSYLNGDYSAENQERVADAYVAGRYGSWSAAKNFWLNNGWY"
     gene            complement(3755..4618)
                     /locus_tag="HMPREF2764_RS07355"
                     /old_locus_tag="HMPREF2764_07375"
     CDS             complement(3755..4618)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268144.1"
                     /locus_tag="HMPREF2764_RS07355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07375"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_070528443.1"
                     /transl_table=11
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGNVISRTSLSKIESGKATPKY
                     ENMEFLLRQINMSFEEFDYICHLYQPSQRTEIIQTYLNMSSIIGNSSLVDFFETCQNYL
                     KTHHDLPIEEIRDMLEVVIHIRQHGTEQLSDQVKQTIKKLWEKIEKQDTWYESDLKILN
                     TILFSFPIDHLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTIYLYNQDKNICKQIC
                     YTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVETH
                     YQPKKL"
     misc_feature    complement(4877..4891)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 17.37"
                     /tool="antismash"
     misc_feature    complement(4939..4955)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     gene            5001..5285
                     /locus_tag="HMPREF2764_RS07360"
                     /old_locus_tag="HMPREF2764_07380"
     CDS             5001..5285
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230167.1"
                     /locus_tag="HMPREF2764_RS07360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07380"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_001230190.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.8e-28,
                     bitscore: 92.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVRIGVASLMVCGILATTNALAEWVDGGQWNYGVGWTGTFGY
                     SDYLHSTRSHTATVKHGGRISKDREDPEVWAQASLNKIPPTGLEYFYGF"
     gene            5340..7448
                     /locus_tag="HMPREF2764_RS07365"
                     /old_locus_tag="HMPREF2764_07385"
     CDS             5340..7448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="HMPREF2764_RS07365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07385"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_070528446.1"
                     /transl_table=11
                     /translation="MKKISHLCMFLLLLCTTFFVFNVNYTREAVRIREMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVIKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKKISNKLGTIPTFLKAKPIQLITFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQSGAILAGFVKTNFYLLLGGSLIIDLGLFLVLDYRPKLLFPSLLLSQF
                     LLLQLYLFISWLTYLMIQKMTVSSMLKGFSSVKLGLIFNYVMKIGTTILLTVLLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFQLNDNLWQEELAGSGKSTDYFYQFYQDLLAKI
                     QVNYVRSASLPIKPVIKAEQVQQYQLPDKVDVYYANSNFLKSKGFKLPDTSTKKVILMP
                     ASDKGQEGKNQFLGKSIAYLSLRYEDQQKQTIEDMDVEIAYYEGDWSFFPYNNERKENL
                     HNPIISLVNDQDMMWEEKTRLSTTGLNNPMKVENTEQNQKVITELVEKLSDGNYLKFSS
                     LQAIQQEKVDSYRDAVRNLNILFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHALLPLLLFAGFTCLDILFVLALASRMEKRSLV
                     ELLKGGIL"
     gene            7445..8074
                     /locus_tag="HMPREF2764_RS07370"
                     /old_locus_tag="HMPREF2764_07390"
     CDS             7445..8074
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593110.1"
                     /locus_tag="HMPREF2764_RS07370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07390"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_070528449.1"
                     /transl_table=11
                     /translation="MIELENITKTIGGKVILDHLSLRIDQGNLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIEL"
     misc_feature    complement(8148..8162)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 19.86"
                     /tool="antismash"
     gene            8824..9945
                     /gene="mnmA"
                     /locus_tag="HMPREF2764_RS07375"
                     /old_locus_tag="HMPREF2764_07395"
     CDS             8824..9945
                     /EC_number="2.8.1.13"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="mnmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /locus_tag="HMPREF2764_RS07375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_07395"
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="WP_070528453.1"
                     /transl_table=11
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWNRVFEYFLAEYRAGRTPNPDVMCN
                     KEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLSQEQ
                     LQKTMFPLGHLEKPEVRRLAEEAGLATAKKKDSTGICFIGEKNFKNFLSNYLPAQPGRM
                     MTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQGFYHD
                     SLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFAEPQRAI
                     TPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     misc_feature    complement(9946..9964)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.46"
                     /tool="antismash"
     misc_feature    9957..9971
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.16"
                     /tool="antismash"
ORIGIN
        1 gttactttat gactattcca gaagctagcc gtttggttat tcacgctggt gcttatgcca
       61 aggatgggga agtctttatc cttgatatgg gcaaaccagt caagatttat gacttggcca
      121 agaaaatggt ccttctaagt ggacacaccg aaagtgaaat tccaattgtt gaagttggaa
      181 tccgcccagg cgaaaaactc tacgaagaac tcttggtatc aaccgaactt gttgataacc
      241 aggttatgga taagattttc gttggtaagg tgaatgtcat gcctctagaa gccatcgatc
      301 aaaaaattga agagttccgc actcttagtg gagatgagtt gaagcaagct attatcgcct
      361 ttgccaatca aactacccac gttgaataaa aaagaaaaac gcatattatc aggtcttgaa
      421 aaaccttggt aatatgcgtt ttgttatgtg taaacttgct tcattttatt ctgatactca
      481 ataaaaatca aagagcaaac taggaggata gccgaaggtt actcaaagca cagcgttgag
      541 gttacagaga aaactaacgt ggttcgaaga gtatgaaatt gagtatttgc ccagtctccg
      601 ttatattatt gaaaatactt caaaacttct aagggtttgt gagcgatata atcaggctga
      661 tagtttagta gatctgcttg ttctccaaat ccccaagtga cagccaattt ctgaatgcct
      721 gtttcttgag ctccaagcat atcaaacttg gtatctccga tgatgatggc ttgttctggt
      781 gctagttgat gtgtctgtaa ggcttggcga atgacatctg ccttatgagg tgcttcaggg
      841 ctggagccat aaatgccatc aaagaaatga tggattccaa agtttttagt catatcttga
      901 gcggtagggg tattctttgt agtggtgata taaagaggat atttgcttga tagctcctta
      961 agcaagtcta caatctgagg aaagagttga gcttcatgga tgccttttgc cttgtagtaa
     1021 gaacggtata tctgaacagc ctcagagatt agttctttgg gaaggcaggt cgcaaaacta
     1081 ctttcgagag gtggtcccat aaaacctcga atagttttgg catcagggct aggcacctcc
     1141 agctccttaa aggtatgggt aaaggcattg tgaatcccga tagaactgtc aacgagggtt
     1201 ccatccagat cgaaaaaaat cgctgtgata gaggtcatgg tttctcctat ttgataaact
     1261 tattctccga aaatttcttt ttggaggcga cgaccagtag gggtggcagc gagtccacct
     1321 tcagctgttt cacgaaaggc agttggcatg cttgctccta cttggtacat ggcatcgata
     1381 acttcatcca caggaatttt agattcgata cctgccaggg ccatgtctgc tgcgatgaag
     1441 gcaaagctag ctcccatggc attacgttta acacagggaa cttcaaccaa gccggcaaca
     1501 gggtcacaga tgaggcctag catattttta atgacaaagg caatggcctg actggcctga
     1561 taaggtgttc caccggcagc tagagtcaag gctgcagcac tcatagcaga ggccgaacca
     1621 acctcggcct gacagccacc ttcagcacct gagatagagg cattgtttgc gatgactagt
     1681 ccaaaggcac cggcagcaaa gaggaaatcc agctgttgct cgtggctgag gtctaatttt
     1741 tcaatagcag ctgttagaac ggaaggcaga cagccagcac ttcctgcggt cggagtggca
     1801 caaaccaagc ccattttggc attgtgttca ttgactgcaa tggcatttcg ggcagcagag
     1861 agaatcgtgt aatctgacag agtttttccg tttttgatgt agtgatccaa tttggcagca
     1921 tctccacctg tcaggccact acgagattta ttttcattga ggccaagttg gacagaggct
     1981 ttcatgactt ctagattgcg ttccatgaga aggaagactt cttcacgttc gcgaccagtc
     2041 aattcaaact ctgttgtaat catgagttct gcgacatttc cttgaaagtc cagttctgcc
     2101 tgctcgacca attctttgat agaataaaac atgcttcctc ctatttaaag aaattgacat
     2161 tgtggagatg agggattttt cgaatttctt cgatagcctc atcacagttg cgactgtcaa
     2221 cttcgataat cataatagct ttttcaccag ctttttcacg agtaacattc atctgggcga
     2281 tattgatatc atagcgggaa agggcctctg ttacgagggc aatcatacct ggaatatctt
     2341 gatgaacaat gatgatagtc ggtgtattca tattgagaga gacggcaaag ccattgagtt
     2401 cggttacctg aatatttcct cccccgatag aaataccagt cacgctaatg gtcttatgag
     2461 catttttaac ggtaatttta gtggtgtttg ggtgaggggc attgctgtct ttctgaatgg
     2521 tccagacaat cttgatgcca cgcttgtggg caatttccag gctgtttggg atttcaggat
     2581 catctgtatc catgcccaaa atacctgcaa caagggctag gtctgttccg tgaccacgat
     2641 aggtcttggc aaatgagtta aatagttgga attcgacttc tgtcggagta tcatcaaaaa
     2701 tggaagagac aatcttccca atacgaacag caccagcagt atggctacta gatgggccaa
     2761 tcataactgg tccgatgata tcaaagacag attgaaaacg aagtgatttc atcagtttcc
     2821 ccttataaaa attcttatct ctattatatc aaagaatgaa ggtcttggct ttaattgtgg
     2881 atgaaaacct ttttacacta caaatagcat aaaaaagcat cttttgtaac aaaaaatagc
     2941 ttgtttaggg aaataaaaaa taattttgta atatttctac ataaaagtgt caagaaacag
     3001 taatatttaa agggtatgat agtactatag aaagaaggag aattttcgaa tatgaaatca
     3061 acaactaaaa agattaaaac aactcttgca ggagtagctg ccttgtttgc agtatttgct
     3121 ccatcatttg tatctgctca agaatcatca acatacactg ttaaagaagg cgacacactt
     3181 tcagaaatcg ctgaaactca caacacaact gttgagaaat tggcagaaaa caaccacatt
     3241 gacaacatcc atatgattta tgttggtcaa gagttggtta tcgatggtcc ggtagcgcct
     3301 gctgcaacac cagcgccaac tacttatgca gcaccagctg ctcaagatga aactgtttca
     3361 gctccagcag cagaaactac agaagtagca gaagaagcag ctccagtggc aagcacacct
     3421 gcagcagaag aaactgttgc tacagcagaa acttcagcac cagctgcaac tgtaagcgga
     3481 tctgaagcag aagctaaaga atggattgca caaaaagaat caggtggtag ctatacagct
     3541 acaaacggcc aatacattgg acgttaccaa ttgacagatt catacttgaa cggtgactac
     3601 tcagctgaaa accaagaacg tgtagcagat gcctatgttg caggacgtta cggttcatgg
     3661 tcagccgcta aaaacttctg gcttaacaac ggctggtatt aagaaataac aaacagaata
     3721 atatgaaaag gtcgaggagc tccctcgact ttttttataa tttctttggt tgataatggg
     3781 tctctacttc ttttttgaga tgagacagca tagaagtttc ttcggttagc tccagaaggg
     3841 agaacccttt ttggataagt ttagaatcgt ctgtacaaat cccaatacgg acatagcaga
     3901 tagcaagcct atcatagctt ttcttgttct tggcatcttc tagtaaagta tagcagattt
     3961 gcttacatat gtttttatct tgattgtata agtaaatggt ggaaaggttt aatagaattg
     4021 tcattcgcaa gtcatataaa tgttggtagt ttttatagac ttctaagcgt tgcaggattt
     4081 ttccagtaat gagatggagg tgatcaatgg gaaagctgaa aaggatggta ttgaggattt
     4141 ttaggtcact ttcataccat gtatcttgtt tttcaatttt ttcccaaagt tttttgatag
     4201 tctgtttcac ttggtctgat agttgctctg taccatgttg acggatatga atgacaactt
     4261 ccagcatatc cctgatttct tctataggga ggtcatgatg ggtcttgaga tagttttggc
     4321 atgtttcaaa aaaatcaact agactactgt tgccaatgat agaactcata ttaagataag
     4381 tttgtatgat ttctgttcgt tggctcggtt gatagagatg gcagatgtag tcaaactctt
     4441 caaaactcat attgatttga cggagaagaa attccatatt ttcatatttg ggagttgcct
     4501 tgccgctttc aatctttgat aggctggttc ttgagatgac atttccacag acctcttctt
     4561 gggttaatcc ttttgactca cgtatttctt tatagacctt tccaaaatca taacgcatga
     4621 ttttctcctt ttagtgaaaa aagttaacaa ataataaaat ctagttaaaa atattgtatc
     4681 atagtaacag aaatagtaaa aaagaaaact aaaatagtgt gtgaaaaaag ttaactaatt
     4741 ttaaaataga gtaaagaaga ggtatactat agatacgaaa tacagatagg agggaaagag
     4801 tatgaataaa aaagtttttc gttttttagt tattcttgct ttcttattca gcattgctac
     4861 ggttttccca tggaactggc cgatataatg cgctagttgt aaaaatgaaa ggaaggtgag
     4921 tccaatggac tacgatactt gttgataaat tactatagct gtttctacgg aacagctctc
     4981 gataatagga gaaataaatc atgagaaaat tagttagaat tggtgttgca tctttaatgg
     5041 tatgtggtat acttgctact acaaatgctt tagcagaatg ggttgatggt ggtcaatgga
     5101 actatggagt aggttggaca ggaacttttg gatattctga ttatttacat tctactcgat
     5161 ctcatacagc aactgtcaag catggaggta gaatctctaa ggatcgtgaa gatcccgagg
     5221 tttgggctca agcttccctt aataaaattc cgccaacagg attagaatat ttctatggat
     5281 tttaagtgaa attttccctc tcctattagg agagggaaaa ctttagtgga ggtactttta
     5341 tgaaaaaaat cagtcatctt tgtatgtttc tgctgttgct gtgtaccact ttttttgttt
     5401 ttaatgtaaa ctacacacgt gaagcggttc ggattcggga aatgggaaag actgtagatt
     5461 ctttggattt gtatttgaaa gatattaacg aacctgcagc gtctgttctt cgattttttg
     5521 aggatgtatc aaaggagtac aaagtctcca tcatcaaaac agatagtggt gatgaggtaa
     5581 tcaagtctgg tgtttttgat aaagatacct tcccctacca agagtttggg atttcttctc
     5641 ttgattttac cacagatggt gaaggagtct acagtaataa aaaaatttct aataaacttg
     5701 gtacgatacc gacctttcta aaagccaagc ctattcagct tataactttt caaacctata
     5761 tcaaggatac atctcgtagt ttaaatggtc gctatacgat aacttctaca caagagatgg
     5821 ataaggatag gattgtacag aaatggagcg attttttcaa gatagaccag gctactttgc
     5881 tagagcctac ctataaaagt gcagtggaag tcataaatcg agatttgctt ttatctgcca
     5941 ttgtttttgt cttggctatt ttacttcttg tgttagtaac agtttatcag ccgatgatgg
     6001 agatgaaacg agtgggggtt caaaagttac ttggttttca aagcggggcg attttagctg
     6061 gttttgtaaa gactaatttt tatcttctct tgggtggaag tcttatcatt gacttgggac
     6121 tatttttagt gctggactac aggccaaaac ttctgttccc aagtttactc ttatcccaat
     6181 ttctgctttt acagttgtat ttgtttatca gttggttgac ttacctgatg attcagaaaa
     6241 tgacagttag ttccatgttg aaaggttttt catctgtcaa acttggtctc atcttcaatt
     6301 atgtgatgaa aatagggaca actattttac tgacggtctt actgattggg gtgggcagaa
     6361 gtctagaaca agaaaacaaa gaacttgctt atcagcaaca gtgggtaagc caagggaatt
     6421 acttgacctt agaaaccttc caactcaatg ataacctgtg gcaagaagag ctagcagggt
     6481 cagggaaatc tacagattat ttctaccaat tttatcaaga tttgctagca aaaatacaag
     6541 taaattatgt gcgaagtgcg agtcttccta tcaaaccagt catcaaagct gaacaagtac
     6601 agcagtacca actgcctgat aaggtagatg tttactatgc taatagcaat tttctaaaga
     6661 gcaagggatt caagttacca gataccagca ctaaaaaagt tattttgatg ccagccagtg
     6721 ataaaggaca agaaggtaag aatcagttct tgggaaaatc catagcctat ctttctctga
     6781 ggtatgaaga tcagcaaaag caaacaatag aagatatgga tgtagaaatt gcctactatg
     6841 aaggagattg gtcattcttc ccttataata atgagcgaaa ggaaaacctc cacaatccaa
     6901 tcattagtct ggtaaatgat caagacatga tgtgggaaga aaagactcgc ttgtcaacga
     6961 caggtttaaa caatcctatg aaagttgaaa atacagaaca aaatcaaaaa gtgattacgg
     7021 agttagttga gaaattatca gatgggaatt atttaaaatt ttcatcgctt caagccattc
     7081 aacaagagaa agtagattct tatcgagatg cagttcgaaa tcttaatata ctctttgctt
     7141 tgtttggtct ccttagcatg atgatttcct acttcttact agtaacaact ttcttattga
     7201 agcgcaggga catcattacc aagaaattta tggggtggaa actggtcgat cgctaccgcc
     7261 ctctcctcgt tctgctcttg ctgggctata gtttccctct tctagtattg attttctttg
     7321 cccatgcgct cttgccactc ctgctgtttg caggctttac atgtctggat atactatttg
     7381 tgctagcctt ggcttctagg atggagaaaa gaagtctagt agagttattg aaagggggca
     7441 tcttatgatt gagttggaaa atattaccaa aaccattggg ggaaaagtga ttttggatca
     7501 cttgtctctc aggattgatc aggggaattt ggtagccatc gttggaaaaa gtggtagtgg
     7561 taagtcgacc ttgttaaatt tattaggttt gatagatggt gattatagcg gacggtatga
     7621 gatttttggt cagacaaatc tagcggtcaa ttctgctaag tcgcaaacaa taatccgtga
     7681 acatatctct tatctgtttc aaaattttgc cctgattgat gatgaaacgg tcgagtacaa
     7741 tctcatgctg gcgctgaaat atgtgaaatt gtccaagaaa gacaagctca aaaaggtgga
     7801 agagatttta gagagagtag gtttgtcagc tactttgcat caaaaggttt ccgagttgtc
     7861 tggtggcgaa caacaacgaa ttgcagttgc tagagccatc ttaaaaccca gccagctgat
     7921 tttagccgat gagccgacag gttcgctgga ccctgaaaat agagatttgg tcttgaagtt
     7981 tctcttagag atgaatcgag aggggaaaac agtcattatt gtgacccacg atgcttatgt
     8041 agcccaacaa tgtcatcgaa tcattgaatt gtaaaaaata cagaaatctt atcaaaaatc
     8101 tttaaattta aggatacctt taaaatttaa ttttaaaaca ctcatttatc aacatttttg
     8161 attgataaaa ataggaaatc agttgttttt gggagattcc ctaaaatcac ctcctaacca
     8221 cacgtactaa aaagatactt cctatagaaa tcgtatgatt ctgaagcttc gaatagttca
     8281 gttttcatga tttttatgct attctagctt tagaatcctt cagaacgtaa aattttaagc
     8341 agtcaaagat ttagaatatc cctaaaacat ttattttatg gcaactctaa acgtagaatt
     8401 gcgtggatat gtatggattt aatctattcg atagttagga cagctctatt ctgggaaatc
     8461 tgagcgatta atggtttaga acagcatttc aacaatggat tttgttcaag gattgattat
     8521 tttataaaag aggccggtag attatcagcc tctttcttgt cttctccaac caagcatgtt
     8581 ataatgaata ctgctcaagc gaccttcaat cgttaagcac acacgacctt caatcgtgaa
     8641 ctagtcattt cgtttatctt ttattacgtc gttaactcgt cttgcctaac tcaagttatg
     8701 cctgcgactc gttgcctagt cctaaaagca aacgaaaaga ctatactcga agaaattcta
     8761 ttgaaagtca tagtaaatag aattttgagg ggtataaata aacgaataga tgggagactt
     8821 accatgagtg ataactctaa aacacgtgtt gttgtgggga tgagtggtgg tgttgattcg
     8881 tcggtgacgg ctcttcttct caaggagcag ggttacgatg tgattggcat cttcatgaag
     8941 aactgggacg acacagatga aaacggcgtc tgtacggcga ccgaagatta caaggatgtg
     9001 gctgcggtgg cagaccagat cggcattcct tactactctg tcaattttga aaaagagtac
     9061 tggaaccgcg ttttcgagta tttcctagcg gaataccgtg cagggcgcac gccaaatcca
     9121 gacgttatgt gcaacaagga aatcaagttc aaggcctttt tggactatgc catgaccttg
     9181 ggtgcagact atgtagcgac ggggcattat gctcgagtgg cgcgtgatga ggatgggaca
     9241 gttcacatgc ttcgtggtgt ggacaatggc aaggatcaga cctatttcct cagtcaactt
     9301 tcgcaagaac aacttcaaaa aaccatgttc ccattgggac atttggaaaa gcctgaagtt
     9361 cgcagactag cagaagaagc aggccttgcg actgctaaga agaaagactc gacaggaatt
     9421 tgctttatcg gagaaaagaa ctttaaaaac tttctcagca actacctgcc agctcagcct
     9481 ggtcgcatga tgactgtgga tggtcgcgat atgggtgagc atgcaggact tatgtactat
     9541 acgattggtc agcgtggcgg tctcggtatc ggtgggcaac acggtggtga caatgcacct
     9601 tggttcgttg tcggaaaaga cctgagcaag aatattctct atgtaggaca aggattctac
     9661 catgattcgc tcatgtcaac cagccttgag gctagtcagg ttcactttac tcgtgacatg
     9721 ccagaggagt ttacgctcga atgtacggct aaattccgct accgtcagcc tgattctaag
     9781 gtgaccgtcc atgtcaaagg agacaaggca gaggttatct ttgcagagcc acaacgtgcg
     9841 attacaccag gacaggctgt cgtcttttac gacggggaag agtgtctagg tggtggtttg
     9901 attgacaatg cttaccgcga tggacaagtt tgtcagtaca tttagattga caaattttct
     9961 caatttgcta caataataaa agcaatagaa atgatggtca aagctcatgg atgttgcagg
    10021 cttttttgtc ctgcacttct ttggagtttt gactgttttt gtgtcgttta gaggaaagga
    10081 caagaatgat tcaacaagac tttcggacaa aaacaggaaa tacagttttt ggagttcggg
    10141 cgacagcctt gattgtccaa aatcgcaagc tcctagtcac caaagataag ggcaagtatt
    10201 acactattgg cggtgccatc caagttaacg aaagaacgga agaagcggta gtccgtgaag
    10261 tgagggaaga actgggtgtc gaagc
//
