LOCUS       NZ_KV802692            12154 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptococcus sp. HMSC073F11 Scaffold236, whole genome shotgun
            sequence.
ACCESSION   NZ_KV802692
VERSION     NZ_KV802692.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptococcus sp. HMSC073F11
  ORGANISM  Streptococcus sp. HMSC073F11
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 75850)
  AUTHORS   Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R.,
            Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A.,
            Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) McDonnell Genome Institute, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 213x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001810785.1-RS_2024_11_05
            Annotation Date                   :: 11/05/2024 23:57:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,892
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 16 of 57
            Pseudo Genes (incomplete)         :: 34 of 57
            Pseudo Genes (internal stop)      :: 10 of 57
            Pseudo Genes (multiple problems)  :: 3 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:06:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 38344
            Orig. end                         :: 50498
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV802692.1.
            The WUSC is a large strain collection isolated from clinical
            samples. Each sample is associated with metadata, including source,
            isolation site, 16s rRNA, metabolic, and other phenotypic
            information. The goal is to sample an adequate number of important,
            yet minor species, further adding to the catelogue of sequenced
            bacterial genomes and improving the diversity of the genomes
            available to the public. WGS will be preformed on approximely 550
            isolates. Samples were selected based on RDP analysis at the genus
            level. This is a reference genomes for the Human Microbiome Project
            and the work was funded by the National Institutes of Health (NIH)
            grant U54 HG004968.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7154)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1066..1076)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    2534..2547
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.09"
                     /tool="antismash"
     gene            complement(2617..2760)
                     /locus_tag="HMPREF2764_RS10375"
     CDS             complement(2617..2760)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605805.1"
                     /locus_tag="HMPREF2764_RS10375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_079211609.1"
                     /transl_table=11
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLRRGLGELTDVYEIVWEKLQE
                     LK"
     gene            complement(2762..3469)
                     /gene="purC"
                     /locus_tag="HMPREF2764_RS06540"
                     /old_locus_tag="HMPREF2764_06560"
     CDS             complement(2762..3469)
                     /EC_number="6.3.2.6"
                     /GO_function="GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /locus_tag="HMPREF2764_RS06540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06560"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_070528193.1"
                     /transl_table=11
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGK
                     GVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVEIIPLEVVLRNYTAGSFSKRF
                     GVDEGIAFETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINELLKA
                     WFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLGELTD
                     VYEVVWEKLQGLK"
     gene            complement(3639..4988)
                     /gene="comB"
                     /locus_tag="HMPREF2764_RS06545"
                     /old_locus_tag="HMPREF2764_06565"
     CDS             complement(3639..4988)
                     /codon_start=1
                     /gene="comB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /locus_tag="HMPREF2764_RS06545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06565"
                     /product="competence pheromone export protein ComB"
                     /protein_id="WP_070528197.1"
                     /transl_table=11
                     /translation="MKPEFLESAEFYNRRYHNFSSRVIVPMSLLLVFLLGFATFAEKEM
                     SLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGELLVQYQEGAEGVQAEAYAS
                     QLDMLKDQKKQLEYLQKSLQEGTDYFPEEDKFGYQATFRDYISQAGSLRASTSQQNETI
                     ASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLASQNLAYSLYQSYKSQGE
                     ENPQAKSQAVAQVEAQLSQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKV
                     GQELTLLDQKILEAESGKKVQGNLLEKGKITASEDGVLHLNPETSDSTMVAEGALLAQL
                     YPSLEKEGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAKNQLFLDSTITSIDATATKTE
                     KGNFFKIEVETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            complement(5001..7154)
                     /gene="comA"
                     /locus_tag="HMPREF2764_RS06550"
                     /old_locus_tag="HMPREF2764_06570"
     CDS             complement(5001..7154)
                     /codon_start=1
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /locus_tag="HMPREF2764_RS06550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06570"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_070528201.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.5e-43, bitscore:
                     138.9, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1124.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYSLAHLRELAKTT
                     IDGTTALGLVKVAEEIGFETRAIRADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTGQD
                     KDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKDQKNGLLSFIPILVK
                     QRGLIANIVLATLLVTLINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQIL
                     SYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMTFFATRRTGEIVSRFTDANSIIDALAS
                     TILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNRNTMEAN
                     AVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRVESQQKALKKVAH
                     LLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRL
                     NEVYLVASEFEEKKTVEDLSLMKGEMTFKQVSYKYGYGRDVLSDINLTIPQGSKVAFVG
                     ISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQPYVFNGTILD
                     NLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARA
                     LLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEKVVVLDQGK
                     IVEEGKHADLLAQGGFYAHLVNS"
     misc_feature    7284..7297
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.85"
                     /tool="antismash"
     gene            complement(7588..7767)
                     /locus_tag="HMPREF2764_RS06555"
                     /old_locus_tag="HMPREF2764_06575"
     CDS             complement(7588..7767)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808742.1"
                     /locus_tag="HMPREF2764_RS06555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06575"
                     /product="hypothetical protein"
                     /protein_id="WP_049515845.1"
                     /transl_table=11
                     /translation="MKTFLLGFAEGYFIVSLIIYIATYLILKNPINTLFYLEIYPVLFG
                     LFYLGYKANKKQVG"
     gene            complement(7848..8063)
                     /locus_tag="HMPREF2764_RS10310"
     CDS             complement(7848..8063)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755479.1"
                     /locus_tag="HMPREF2764_RS10310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_078934268.1"
                     /transl_table=11
                     /translation="MNSRNLNLNTLLPKLATICPLLGITLNVALHVIRTGSLVSFNWQW
                     TLVGLLFAGYFGIFRKDLSKNNQNYK"
     gene            complement(8067..8222)
                     /locus_tag="HMPREF2764_RS06560"
                     /old_locus_tag="HMPREF2764_06580"
     CDS             complement(8067..8222)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357249.1"
                     /locus_tag="HMPREF2764_RS06560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06580"
                     /product="smi_0061 family bacteriocin-like peptide"
                     /protein_id="WP_000357248.1"
                     /transl_table=11
                     /translation="MDKFQVVDEKDLQVIEGGIEPISIIFGLSAIAFGAFGAGYTFGSD
                     LARRGR"
     gene            complement(8436..8633)
                     /locus_tag="HMPREF2764_RS06570"
                     /old_locus_tag="HMPREF2764_06590"
     CDS             complement(8436..8633)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180819.1"
                     /locus_tag="HMPREF2764_RS06570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06590"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_049502335.1"
                     /transl_table=11
                     /translation="MTNFDKMEQNFVALTEEELMNVDGGIAPIIIGGVVISWKLIGGVA
                     ALATASAGAGIAAGYYANRP"
     gene            complement(8667..8822)
                     /locus_tag="HMPREF2764_RS09735"
     CDS             complement(8667..8822)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004580.1"
                     /locus_tag="HMPREF2764_RS09735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_049499436.1"
                     /transl_table=11
                     /translation="MNEMNDKCTIEVLKESDLALLVGGSKESYQRGLITGLLLRSVFIG
                     TPFFRG"
     gene            complement(8833..8961)
                     /locus_tag="HMPREF2764_RS06575"
                     /old_locus_tag="HMPREF2764_06595"
     CDS             complement(8833..8961)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658145.1"
                     /locus_tag="HMPREF2764_RS06575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06595"
                     /product="smi_0059.1 family bacteriocin-like peptide"
                     /protein_id="WP_049499438.1"
                     /transl_table=11
                     /translation="MKEHYYGFVELTSEELTDIQGGENWTKTLADWYVGFRRGLGF"
     misc_feature    complement(9036..9049)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    complement(9037..9050)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    complement(9162..9175)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    complement(9163..9176)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     gene            complement(9334..10065)
                     /locus_tag="HMPREF2764_RS06580"
                     /old_locus_tag="HMPREF2764_06600"
     CDS             complement(9334..10065)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221865.1"
                     /locus_tag="HMPREF2764_RS06580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06600"
                     /product="response regulator transcription factor"
                     /protein_id="WP_070528204.1"
                     /transl_table=11
                     /translation="MRILVLEDDRVQQGRIEQTLLDIGRSRNLRLEIDIAKNYGDVEKY
                     SQYFDHYQLYLLDLEIDGERERGFQVAQQVRERDPFTSIVFVSTHSEALPLAFRYHLSA
                     LDFISKDQPEENYRHQLERCLDYVLAVDKRENMRLFTYSFEGRRGFTLPYHEILAFETS
                     VESHRIKVYYANHSIKTIYGSLKDIAAKAEKDYFVYANRNTLVSLHAIEEMTATEVTLL
                     EGLHFPVSRTARRTLKKHLNV"
     gene            complement(10062..10877)
                     /locus_tag="HMPREF2764_RS10315"
     CDS             complement(10062..10877)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.5"
                     /locus_tag="HMPREF2764_RS10315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_239822585.1"
                     /transl_table=11
                     /translation="MTNRNSSFYVRFDTTISLLIFILFFSWLFYIKHLEQVYRDEQTIQ
                     RQEDENRSLQGMVDKLGHLYDEVRGFRHDFAGIVASMEPAIANQDMAEVSTIYQDVFLK
                     TNEKLRKADYTAFNLHNIHDIAIRNTLAKAMIVADNQGIHFSLETVGVVEELALPMLEA
                     IRILSILTTNALEAASEAENPQIRVALLAGDRSVRFIIENTRKKEELNPSILSQKGYST
                     KGNHSGLGLATLEDMVFHYDLNLDTQLGETTFRQDLELPFKDEKREGEE"
     misc_feature    complement(11294..11307)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.03"
                     /tool="antismash"
     misc_feature    complement(11552..11566)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: strong, score: 25.16"
                     /tool="antismash"
     misc_feature    complement(11695..11710)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 22.93"
                     /tool="antismash"
ORIGIN
        1 ttgtgagcct tttgaagggc ttcaaactga gcaaaatttt tcttaatcaa atcaaaatca
       61 atctctgtag caagagcgtg acctgggatg tagtagatat tttccccaac agctttaaat
      121 tctggagaga gcacattacg gctatctgcc gtcgtcaccc caaaggcaac caaggttggc
      181 ggtactgtca attcttcaaa ggtaccagac atagagtcct taccaccgat agatggcaaa
      241 ccaagctgaa tttgtgcctc gatagagcct aggagagctg ctactggctg accaaaacgc
      301 tcagcttgtt tgtccatgcg ctcgaaatac tcttggtaag agaaacgagc cttagaccag
      361 ttggcaccag cagccaccaa acgagctgtc gcttcgatca ccgcataggc agcaccgtgg
      421 tatggagacc attctgctac ataagggttg aaaccttggg ccataactga cgccgtatga
      481 gtcactccgt gttgaactgg caatttctga acagaagcct cagttggtgt gagttggtag
      541 cgaccgccaa gtgggtgatt gatcgtcgaa cgaccaacag agcaatcaaa gatggtctgc
      601 aaaccttttt gactcgcatg gttgaggtta gatagaacgg taagggtatc tgcttccagt
      661 gtttctacgc ttgttgtacg ctcctctgga agtttgacgt ccttgtccac aaccttagca
      721 tcgacaacta cgcgcacacc attggtatca aggaaacggc gttccaagtc gacaatcgtt
      781 tcaccattcc agtgcatgac aagatttggt ttttcagtca ctgtcgccac aacaacagca
      841 tcaatatttt ctttgttaca ttctacaacg aaggcatcta cgtcttctgg acgtaccaca
      901 acagccatcc gttcttgaga ttcagagatg gcaatttcag taccattcaa tccttggtat
      961 ttaagaggta ctttgttgag gtcgatttca agaccgtctg ctaattcacc gatagccaca
     1021 cagacaccgc ctgccccaaa gtcattagat ttcttgataa gacgagtgac attgccatta
     1081 cggaaaaggc gttgaatctt gcgttcttcg atagcattcc ctttttgaac ttcagctcca
     1141 gcagtttcca cagactcaac tgtttgaacc ttagaagaac ctgtcgcacc accgacaccg
     1201 tcacgtccag tcttaccacc aagaaggata atgacatcac ccgcttccgg tttttctcgg
     1261 acgacgttgc ccttaggagc cgcaccaaca acagcaccta gctccatgcg tttagctaca
     1321 aatcctgggt ggaaatactc gcgaacgtag gttgttgcaa gcccaatctg gttaccatat
     1381 gaagaataac catgagccgc tgttttagaa ataacctgtt gtggcaattt accagcacgc
     1441 gtttcagaga ttggtgctgt aatatcacct gcacccgaga tacgcatggc ttggtagaca
     1501 tatgagcgac ctgacaacgg atcacgaatg gctccaccga tacaagttgc cgccccacca
     1561 aatggttcaa tctcagttgg gtgattgtgg gtttcatttt tgaacatgag gagccaaggt
     1621 tccttgatac catcaacgtc cacttcgatt tctactgagc aggcgtttat ttcatcagac
     1681 acttccatat cgtccaaacg tccattagca cgctcataac gaccgaaaat agtcgccata
     1741 tccatcaagg tttgtggttt ttcagaacgc cccaactcat cacgcatggc aatatatttt
     1801 tcataggttg actgcaattg tttttggaat ttagaagctg aaaagtcgat gtgtttcaat
     1861 tcagtctcga aagtggtgtg acggcagtgg tcagaccagt aggtatccaa caccttaagc
     1921 tccgtctcag tcggcacgcg cccgattgac ttaaagtagt cttggataaa gagcaaatca
     1981 tccacttcca ttgccatccc ttgctcggct ttgtagcggg caaaatcttc tgctgtatag
     2041 ctttcaaaga aagtcaattt aggaatggtc ttgtctgact ctgaaaattc ctgcttggca
     2101 atccctgtcg tgatgtcctt gaaacgagaa tcaactggat tgagcaagta gttcttgacc
     2161 gcttccaact cagtcgcatc aatatcttta ttcaccaagt aaagttgggc tgtattaacc
     2221 gttacatcac tcgaacttcc tagtaaaagc aaggcttcct gtgaagaagc cgcacgctgg
     2281 tcaaactgcc ctggcaggct ttcgatggca aagaaagcat agttagcaag atccgcctgc
     2341 acagccgctt cgtccaagac gtggtctgtc acctgctcag agaagatgtg tttctctgca
     2401 ggcgcaaaca ggtccttagc caagttaaag acatcataca cttgcacaat acgaatactt
     2461 ttcaagcttg aaattcccaa gttatgctgg agttctctaa ccaaactctc tgatttgacc
     2521 tgaaaatcag cctttttttc aacaaaaata cgtttatcca tcaattctta ttcctttatt
     2581 tcagctcttg caatatcccc aacgaccttg aggttgttat tttagttctt gcaacttttc
     2641 ccaaacaatc tcgtaaacgt ctgttagttc tcccaatcct ctacggagtg aaaaggtctg
     2701 ggggaccttt tcagcctgag tccttttagt tgagagacga aggttttccg ttgcaaacag
     2761 attatttcaa accttgcaac ttctcccaga caacttcata aacgtctgtt agctctccca
     2821 atcctctacg gaaaacatcc ttatccatgt ggttgccatc cgcatcccac aaacggcagt
     2881 tatctggtga aaactcgtct gccaagataa tcttgccatc cttgtcaaaa ccaaactcta
     2941 gcttaaagtc aatcaattta aggccaatct cagcaaacca agctttcaag agctcattga
     3001 tacgacgagt ttcttccttc aagtaagcga tttgctggtc atccgcaatc tgtaggaatt
     3061 tcacatgctc gtcattgata aatgggtcat ccaaatcatc atttttgtag taaaattcaa
     3121 caatcggagt ctcaaatgcg attccttcat ctacaccaaa acgttttgaa aaggaaccag
     3181 ccgtgtagtt gcgtagcacg acttccaaag gaatgatttc aaccttttta ttgagttgtt
     3241 ctgtatctga aagtttctcc acaaagtgag tcgccacacc agccgcattt aatttctcaa
     3301 aaataaaaga tgaaatctga ttattcaaaa ctcctttgcc cgcaatctgc tccttcttaa
     3361 caccattgaa agcagtcgcc tggtccttgt aagttgaaat aataagattt tcatcctcag
     3421 ttgtatagat atctttagct tttcccgaat agatcaattg ttttgacatt ttaaagttcg
     3481 cctttcgtat tacttgagca cattctacca taaaaaaact aaaattacaa gaacttaacc
     3541 gaataaataa tgtagtgaca ccaaaaatca taaagaaaaa accgcaagaa gaatctttct
     3601 cacagtttaa aaatcattta actctaaaaa cacgaacatt actctttgtt caaaaattga
     3661 tccaaataat aacgcaggta acttttcttg cccgtaatca tctgcaggcg accttccacc
     3721 ccataccgaa gtttttcagc ctgctccgaa gttagattag tctccacctc aattttaaag
     3781 aaattccctt tctcagtctt agtagctgtc gcatcaatac ttgtaatagt agaatctagg
     3841 aaaagttgat tcttggcatc atgagtagtc gtatagcgaa cagaatcacc gaccttgatc
     3901 cttgctacat cttttgaact tagataagct gtgagtttgg ctttcccttc tttttccaag
     3961 gacggataga gttgagctaa taaggcacct tctgctacca tagtagaatc actagtctca
     4021 ggattaaggt gaagcacccc atcctcactc gccgtaattt tccccttttc taaaagattt
     4081 ccctgaacct tcttacccga ttctgcctcc aaaattttct ggtctagaag ggtcaattcc
     4141 tgaccaacct ttgccaagtg ttgggatttg agagactcca attgactgct taaacctgac
     4201 gcataggctt gctgggtacc tgaacctgca tactggacac ggtaagtagc aagactagat
     4261 tctaactgag aaagctgtgc ttctacctgc gcaacagcct gggatttagc ttgcggattt
     4321 tcctcgccct gagacttgta ggactggtag agagagtagg ctagattctg actggccaag
     4381 gaagtgcctg tttcaatagc tgacttagct gtctggtaat cgcgaatttt agcctctgtt
     4441 tggctgataa ggttgccgat ttcagcttgg gtttgactag ctgctgcatt ctgggacgcg
     4501 atggtctcat tttgttgcga tgtactagcc ctaagactgc ctgcttgact gatgtagtcg
     4561 cgaaaggtgg cttggtagcc aaacttatcc tcctctggaa agtaatccgt cccttcttgc
     4621 aggctctttt gcaaatactc caattgcttt ttttgatcct tgagcatgtc caactgacta
     4681 gcataggcct ccgcttggac accctctgcc ccttcctgat attgaaccaa cagttctccc
     4741 ttttttacca gcttattttc ttccaaatga ttaacaagaa tgcgattgtt gctagtagac
     4801 tggatatttg caaggattcg actaggctca acagtagctc tagtagacaa actcatctcc
     4861 ttctctgcaa atgttgcaaa tccaagcaaa aacacaagca gaagggacat gggaacaatc
     4921 acccgactgg aaaaattatg gtaacgacgg ttataaaact ccgcactttc taaaaattct
     4981 ggtttcatcc tctcctcttt ctagctattc accaaatggg cataaaagcc accctgtgca
     5041 agcaaatcag catgctttcc ttcttcaaca atcttgccct gatccaagac aacaaccttc
     5101 tctgtccgct cagcaatggt caagcggtgg gcgatgaaaa tcaaggtctt gtctaaagcc
     5161 atgagattat cgacaattcg cttctcagtc aagatatcca gactgctggt cgcctcgtcc
     5221 aaaatcaaga ccggtgcatc tgtcaagaga gcacgcgcca gagcgattct ctgccgttga
     5281 ccacctgaaa ttcctgctcc atccgaagtc aattccgtct ggtaatttag tggcatgcgc
     5341 tcaatgtcct cccgaatctc tgccaattcg accgcccgta agatatcttc ctgtgtcgtt
     5401 ccctccttgg ctccaaggag aagattgtcc aaaatcgttc cgttaaagac atagggctgt
     5461 tgaggcagat agttaatata ctggcgcagg gcctttttgt caatctgatt gagattgaca
     5521 ccacccagac taatctctcc ctgacttggg tcgtaaaaat taaccatcat tttggccaag
     5581 gtcgtcttac ctgatcctga aatccccaca aaagccacct tagaaccttg gggaatggtc
     5641 aaattgatat ccgacaaaac atctcgacca tagccatact tgtaggaaac ctgcttgaaa
     5701 gtcatctctc ccttcatcaa actcaaatcc tcaaccgttt tcttctcctc aaactcagaa
     5761 gctaccagat aaacctcatt cagacggtta ttggcaacct gcgctgtctg aagcttggtt
     5821 tgcaggttga tgatattttc caaaggatta gtaaagtaaa ccagcaaggt attataggta
     5881 atcaactgcc ccaaactcat cttgccatcc ataaccagaa cagcccccat ccatagaata
     5941 ccgacattga gcaagagatg ggcaactttt ttcagagcct tttgctgact ctctacccga
     6001 ctataggtaa aggatttctt cagataatcc acaaattcct tatcaatctt ttggtaacgc
     6061 tgactttcac tagtcaaaga cttaatggtc tcaataccgt tgatgtcctc gatgatagaa
     6121 gaagacagaa ccgcattggc ttccatggta ttccgattca tcttttcaaa cggcttcata
     6181 aaggcaaaga taatcactgt gtagatagga agcgccaata aggtcatgaa aaagagattg
     6241 gtattttgtg aaaataaaac aagggaaata atgacgaccg ttgacacgtc caagaaaatc
     6301 gaaagaatgg tcgaagccag cgcatcgatg atactgttag catccgtaaa acgagacacg
     6361 atttcccctg tacgacgtgt cgcaaaaaag gtcataggca ggtgaaaaac atgcttgata
     6421 taggacaaaa ttacatcaat cgacaagcgt tgccccaaaa caagcaagag atactcctga
     6481 gcataagaca agatctgctg gaggatatag acgatgacca aaccaataga aataatcccc
     6541 aacgtcgaac gcatctgatc tggcacatag gtatcaatta tagactgcag ataataagaa
     6601 cccacaatgt taatcaaggt taccaagagt gttgccaaaa cgatattagc gatcaagcca
     6661 cgctgtttca ctaatatagg gataaaagag agcagaccat tcttttgatc cttatgaggc
     6721 ttgtagtctg gactcggtgc cataaacaga gtcactcctg tccattcttc cgcaaaacgc
     6781 tcacgtggca gtttagtcaa cttcaccccg ggatctggat cggcaatatg aatgctgtcc
     6841 ttatcctgcc cagtcaccac atagtagtgg agcaatttcc cttccttaag tacatgagca
     6901 acaaaaggaa aggtcaaatc tggcaagtca aagagcgtca tatctgccct aatagctcgc
     6961 gtctcaaagc caatctcctc tgccaccttg accaagccca aggccgtcgt cccatctatg
     7021 gtcgtctttg ccaattctcg taagtgagcc aaagaataat agctaccata gtagccaaaa
     7081 accatggcta atgaagctac accgcagtcc atctgatcca cctgcggacg atagtgacgt
     7141 ttcccaaatt tcatattcat ctcctttttt ctaaactcat ctgataatac ctatcttacc
     7201 ccaatcccca aacaaaaact tgacttcctt cccaaatgcg cctatctccc tcccaactgc
     7261 acttttggga taaaaaaata ggcagaacaa atgtcctgcc tactaaaaat atggtatgaa
     7321 aggaaagtga ttttttactg acaccctagg caagcttgga acaaataaat cattgatagt
     7381 aacattgtga catcctattt attacttcaa tttctaatgt ccctgctatt tctaagccac
     7441 ctctggtatg atctattcat agtcagaata gataacaccg ttctaaatta cgattcatag
     7501 ttactccttt taaactccat tgtgatgcat ccttagattc taccccagcc atttttcagc
     7561 gaaggctaac aagtcattct taactgttca ccctacttgc tttttattgg ccttatatcc
     7621 tagataaaag agcccaaata aaacagggta aatttctaga taaaataggg tattgatcgg
     7681 atttttcaaa atcaaatacg ttgcgatgta gattatgagc gatacgataa aataaccctc
     7741 agcgaagcct aataaaaatg ttttcaagcc agactcctat ttacctgaca agaatggcgg
     7801 aaaaacgcta ccattctcat tcacaaaatc cttttgattc atgttcttca tttatagttt
     7861 tgattatttt ttgacaaatc tttgcgaaaa ataccaaaat atcctgcgaa aagcaaacca
     7921 actaaagtcc attgccaatt aaaggatact aacgaacctg tacgaatcac atgaagcgcg
     7981 acattaagag ttattcccaa caaaggacat atcgtggcta atttaggtaa caatgtattg
     8041 agattaagat tgcgactgtt cattatctaa cgcccacgac gagccaaatc tgaaccaaat
     8101 gtgtaaccag ccccaaatgc gccaaatgca atagcactta ggccaaatat aatcgaaata
     8161 ggttcaattc ctccttcaat tacttgcaaa tctttctcat caactacttg aaatttatcc
     8221 attcttttct cctttacttc tatataataa aatgaatacc attaaaaaca atgaaattgt
     8281 tggtatatta aaatcatcaa accatgtagg taagggtagc ttattcacat cagctaccaa
     8341 cctgataatt gtataagata ttgaactaag taataccata agctcaggcc aaatcttcag
     8401 cattaattta acaaatctca tatataaatc caaaattatg gtcgattagc ataatatcct
     8461 gcagctatgc ctgcacctgc tgaagctgta gcaagcgcag ctacacctcc aattaatttc
     8521 caactaataa ctacaccacc aataataatt ggtgcaattc ccccatctac attcatcaac
     8581 tcttcttctg taagagctac aaagttctgt tccattttgt caaaatttgt catctttttc
     8641 tcctcttatt ctattttttc taaatcttat cctctaaaaa atggagttcc aataaataca
     8701 ctacgtaaca atagacctgt aattaaaccc cgttgataac tttccttgct accacccacc
     8761 agcaaggcta agtcgctttc ctttaatact tcaatagtac acttatcatt catttcattc
     8821 atcgcttatc ccctaaaaac ctaaacctct acgaaatcca acgtaccaat ctgcaagtgt
     8881 cttcgtccaa ttttctccac cttgaatatc ggtcaattct tccgaagtca gctcaacaaa
     8941 tccataataa tgttctttca ttattttatt cctttctttt ttccctctca cttatctatt
     9001 cgaaaaacaa tccgaaaatg aacaactaaa ttaaatttta tttaaaaaat aatttaatga
     9061 accacggata caagattggt agatacataa accaggctcc tttccttttc ctcttactta
     9121 tctattcgaa aaacaattcg aaaataaaca actaaatcaa attttattta aaaaataatt
     9181 taataaacca cggatacaag attggtagat acatgaacta ggctcctttc cttttcctct
     9241 tacttatcta ttcgaaaaag aagtcaaaaa aataagaata tcagaaaatt ttccgacatt
     9301 cttattgata atttgattca agtctaacag atattagaca ttgagatgtt ttttaagagt
     9361 tcttctggct gttcgtgata cggggaagtg caagccttct aacagggtca cttctgtcgc
     9421 agtcatctct tcaatggcat gtaaactgac caacgtattg cgattggcat agacaaagta
     9481 atctttttca gccttagcag cgatatcctt gagactgcca taaatggtct tgatagaatg
     9541 attggcataa tagaccttga tacgatggga ttccactgag gtttcaaagg ctaaaatctc
     9601 atggtagggc agagtaaagc cccgtcttcc ctcaaaacta taggtgaaga gacgcatatt
     9661 ttccctcttg tccactgcca gtacatagtc cagacagcgc tccagctgat gacggtaatt
     9721 ctcctcaggc tgatccttgg aaataaaatc caaggcagac aagtgatagc gaaaagctag
     9781 aggcaaggcc tccgagtgcg tggacacaaa gacaatgctt gtaaaaggat cccgctcccg
     9841 aacctgttga gcaacctgaa aaccccgttc acgctctcca tcaatctcta aatctaataa
     9901 atagagctgg taatggtcaa aatactgtga atacttttca acgtctccat agtttttggc
     9961 aatatcaatt tccaatctca aattgcgaga gcgaccaata tctagtaaag tctgctctat
    10021 acgcccctgc tggactctgt cgtcttctaa aactaaaatt ctcattcctc tccctctcgt
    10081 ttctcatcct taaatggcaa ttctaaatct tgtctaaagg tcgtctctcc tagctgggta
    10141 tctaagttta aatcataatg aaaaaccatg tcctccaagg ttgccaagcc cagtccactg
    10201 tggtttccct tggtagagta acccttttga ctgagaatgc tgggattcaa ttcttctttc
    10261 ttacgagtgt tttcaatgat aaaacggaca ctcctatcac ctgccaacaa agcaacccga
    10321 atctgcggat tttcagcctc actggctgcc tctaaggcat ttgtcgtgag gatagaaagg
    10381 atacggatag cctccaacat aggcaaagcc agctcttcta cgacccccac cgtctccaaa
    10441 ctaaaatgaa tcccctgatt atctgccaca atcatggctt ttgccaaggt attgcgaata
    10501 gctatatcat ggatattgtg gagattaaag gccgtgtaat ccgccttcct cagcttttca
    10561 tttgtcttta gaaagacatc ttgataaatg gtagacacct cagccatatc ttgattcgct
    10621 atagcaggct ccatgctagc gacaattcct gcaaaatcat gacggaaacc tcgaacctca
    10681 tcatagagat gacccagctt atccaccatc ccttgcaagg aacgattttc atcttcctgt
    10741 ctctgaatgg tttgctcatc acgataaacc tgctccaaat gcttgatata aaagagccaa
    10801 gagaaaaaga gaatgaaaat caggagagaa atggttgtgt caaaacgaac gtagaaagaa
    10861 ctatttctat tggtcataaa cgaagagaat atcctaaatc cagtcagagg tagaataatg
    10921 aaaaaactct tatgatagtg ggttttgaaa gcatctgaaa aaaataaatc caaatccaaa
    10981 gaccaacgct tcataatttt acaacataga aatattgata ataaatcaaa tatagatata
    11041 tatagcgtcg aaaactcatc taaaaacaac cagctctcac cagataatat acgaagccag
    11101 attgtcacaa ataaataacg aatactttga aagaagagaa aaagatatag agaaagaaaa
    11161 agagcttcta cttttctctt atgcaatacg agtaaaaaaa tacagtaaaa gaagagaagg
    11221 attcctatat gaaataattc tccaaatgtt tgacgagtgg ataaactaat gaaaaataca
    11281 atgatccaat aacgtttttt ataaaattct ggggaaattt gagcgcccaa ataaatagta
    11341 ggcaccatgg ttgaaacgac taccaacaga atatatagca cagaagctaa gaattctaaa
    11401 ataatcatag aataaaacga ctcctaattt atttaagcga aataatattt atctccccga
    11461 tacaactctc tagtttttca tactttctga aaaacgataa atccacaaaa ggctttagtt
    11521 tcttttaaaa tacaagaaat ctaaagcctc ttgaaaatga tttgcacgtt ttatttttta
    11581 tattcgttgt gaagattgct tatctattta ttttaccaca agaaccttgt aatattcata
    11641 cttatttggc tgtgtaatct taattttttg accatagaaa ccatcgattt ctttgtcaaa
    11701 ataatctgaa aaccttgatg gcaagcgttt catcttaaga acctcaccag attggtctgc
    11761 atatacaagg aaatgatagt gggttgtagc cataccatca tcaatcagta caaagtaacc
    11821 tgttttgagc ttaccttgtc cattatctaa atagtatagt ttattgttat tgattgtgaa
    11881 gttatcaaat cgaagtcgta cacctgggtc ttctagataa agatcattgc caactttttt
    11941 gatttctgat ctagttgtca tctctggaag tggtaatact ctacgaccat ctgctccaaa
    12001 gtaatatcga cctggtagat tttggaaatt acgaataaca gctttaggat caatttcctg
    12061 tccttgatct ttattatctg ttaaagccaa catcgtattg gaaacgtaat ccccatattt
    12121 attagaaaac tttttaaact ctggtttgat gtac
//
