LOCUS       NZ_KV802691            10165 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptococcus sp. HMSC073F11 Scaffold217, whole genome shotgun
            sequence.
ACCESSION   NZ_KV802691
VERSION     NZ_KV802691.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptococcus sp. HMSC073F11
  ORGANISM  Streptococcus sp. HMSC073F11
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 128053)
  AUTHORS   Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R.,
            Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A.,
            Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) McDonnell Genome Institute, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 213x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001810785.1-RS_2024_11_05
            Annotation Date                   :: 11/05/2024 23:57:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,892
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 16 of 57
            Pseudo Genes (incomplete)         :: 34 of 57
            Pseudo Genes (internal stop)      :: 10 of 57
            Pseudo Genes (multiple problems)  :: 3 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:06:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 46168
            Orig. end                         :: 56333
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV802691.1.
            The WUSC is a large strain collection isolated from clinical
            samples. Each sample is associated with metadata, including source,
            isolation site, 16s rRNA, metabolic, and other phenotypic
            information. The goal is to sample an adequate number of important,
            yet minor species, further adding to the catelogue of sequenced
            bacterial genomes and improving the diversity of the genomes
            available to the public. WGS will be preformed on approximely 550
            isolates. Samples were selected based on RDP analysis at the genus
            level. This is a reference genomes for the Human Microbiome Project
            and the work was funded by the National Institutes of Health (NIH)
            grant U54 HG004968.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10165
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10165
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10165
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5165](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5165)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(830..845)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            complement(987..2177)
                     /gene="metK"
                     /locus_tag="HMPREF2764_RS05965"
                     /old_locus_tag="HMPREF2764_05980"
     CDS             complement(987..2177)
                     /EC_number="2.5.1.6"
                     /GO_function="GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="metK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /locus_tag="HMPREF2764_RS05965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_05980"
                     /product="methionine adenosyltransferase"
                     /protein_id="WP_070527971.1"
                     /transl_table=11
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAET
                     AVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSPDI
                     AQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPISLSHKLVRRLAEL
                     RKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEVSNEQIHNDVINKVIKE
                     VIPASYLDEQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDAT
                     KVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGRVAESKLEK
                     AARQIFDLRPAGIIQMLDLKRPIYRQTAAYGHMGRTDIDLPWERLDKVDALKEAVK"
     gene            complement(2971..3270)
                     /locus_tag="HMPREF2764_RS05970"
                     /old_locus_tag="HMPREF2764_05985"
     CDS             complement(2971..3270)
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361392.1"
                     /locus_tag="HMPREF2764_RS05970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_05985"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_070527974.1"
                     /transl_table=11
                     /translation="MDKRTEEILNKVYNLILDTEIHKNERDILLRYKTLLENTKNEQRV
                     VMELSEALRQQAVSGIHSHKSMSPKVATFYKEIATYGELSKNLAQGLISFGITR"
     misc_feature    3383..3397
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 25.88"
                     /tool="antismash"
     gene            complement(4548..4940)
                     /locus_tag="HMPREF2764_RS05980"
                     /old_locus_tag="HMPREF2764_05995"
     CDS             complement(4548..4940)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HMPREF2764_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HMPREF2764_05995"
                     /product="hypothetical protein"
                     /protein_id="WP_070527977.1"
                     /transl_table=11
                     /translation="MESGEFRLILALFISFFIIGAIGIVLNVPISEVLVLSVGSVFFEL
                     LIHLLTHNKKRGNISFELCFNRYKQFIKKILAPTFFLLLLSIIFVVIDGYKSSSVLFYT
                     HIFVVLFTVVYIICSNNIELHISKKE"
     gene            complement(5001..5165)
                     /locus_tag="HMPREF2764_RS05985"
                     /old_locus_tag="HMPREF2764_06000"
     CDS             complement(5001..5165)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448735.1"
                     /locus_tag="HMPREF2764_RS05985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06000"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_000448735.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.5e-10,
                     bitscore: 34.4, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METKTTIQFDVLDTEMLASIEGGTASAGEVCTLAGAMIGSLVAPR
                     AGTWAGGVL"
     misc_feature    complement(5406..5423)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(5921..7246)
                     /locus_tag="HMPREF2764_RS05995"
                     /old_locus_tag="HMPREF2764_06010"
     CDS             complement(5921..7246)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575876.1"
                     /locus_tag="HMPREF2764_RS05995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06010"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_060627929.1"
                     /transl_table=11
                     /translation="MIFFLDIIGTFLMLCIHARVLGERLNLKKIGISIILFYLSILLFV
                     FVFGSTEFYFYGSLLSYPTFFILYTLSIGELRSKVSLLLFYSLFPLGFWDVIKNFLGYF
                     VIYKIPILQTIYETNLGIMIFSLLAKIIVLFLISIFQYNFSHLKIKNLDRKTKFILITA
                     DILMIAYFILPPYINYIDKVSWDGMLDYKELLTAVYFLLFICFVNLLDRKLLQELQEKV
                     VLQKEIQLQNLSNYSQQVEGLYQEIRSFRHDYANILSSLKIGIDQKDINLVSQVYDSVL
                     KNSGEKLKGKRFDVAHLRNINDLALKSLLLSKLSEAQNLSVPVSLEISEQFSIKNMEQI
                     DFLTITSILLDNAIESAAEGGIAVCFLEDTEWNKLVMIVKNSTLEREIELRDIFKRDNS
                     SKGVGRGIGLANVRRILKSYPNVCLKTTSKNYVFTQILEIEL"
     gene            complement(7257..7997)
                     /locus_tag="HMPREF2764_RS06000"
                     /old_locus_tag="HMPREF2764_06015"
     CDS             complement(7257..7997)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019941.1"
                     /locus_tag="HMPREF2764_RS06000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06015"
                     /product="response regulator transcription factor"
                     /protein_id="WP_060627927.1"
                     /transl_table=11
                     /translation="MNIFILEDNFLQQTRIENVVKKILVDNKIEYRHFEVYGKPQQLLE
                     DISERGNHQLFLLDIEIKDADKRGLDVAREIRKLDSQAVIAFVTSHSEFMPVSFEYLVS
                     AIDFIDKELPEPLFIKRIENVILSVSDNQGITVSEDSFIFTGSKAQIQVPFKDLLYIET
                     SSIAHKLTLYSKRDIVEFYGQLSEIIEQEPRLFQCHRSFIVNPYNISSIDKENRVVHFQ
                     NGSSCIVSRLKIRSLMKAVERLHD"
     gene            complement(8008..8340)
                     /locus_tag="HMPREF2764_RS06005"
                     /old_locus_tag="HMPREF2764_06020"
     CDS             complement(8008..8340)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656277.1"
                     /locus_tag="HMPREF2764_RS06005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06020"
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="WP_070527983.1"
                     /transl_table=11
                     /translation="MKDYILYQDRAIVKVSLSKIYYVTTHPTKAHAVLFVTAEGNFEAS
                     TSLAKIEEESSEELIRCHRKFLVNKDKIAGFNHETRTIMFLDDRVSDIACSRRHFTILK
                     NQWKNI"
     gene            8541..8939
                     /locus_tag="HMPREF2764_RS09700"
                     /old_locus_tag="HMPREF2764_06025"
     CDS             8541..8939
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849422.1"
                     /locus_tag="HMPREF2764_RS09700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_06025"
                     /product="hypothetical protein"
                     /protein_id="WP_083306603.1"
                     /transl_table=11
                     /translation="MKAIQILHYSLFGYILSLYLKYYFFNKTFKNTKILQGHKINHKYK
                     SSTYFIIKSRSLTSKKMKLYLKSIQFSSKKSEVIIIGSQIDYDELYRNHYSVFGVIDIT
                     NNKSLKYIKDKIHFYLEELYEFKKDKSD"
     misc_feature    complement(9266..9282)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.17"
                     /tool="antismash"
     misc_feature    complement(10005..10021)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: medium, score: 26.74"
                     /tool="antismash"
ORIGIN
        1 aagaaagttg ccttgatacc atacttggaa agagtatcta atgcttgatt tgtcgtcgta
       61 ggatttggtc catcatcaaa agtcaaggcc actactttac gatttttctt ttcataataa
      121 gccttatata gctctgcatc cttatctaat agataagagg actgaataac ttcaaagaaa
      181 ctagatactg gcaaggcaat ctcgtctaga ttttcaactg actgacttgg ataaaggata
      241 atctgactat ccttgtaatc aaaattccat gcagacaagt cttggtcaga gaagccttta
      301 acaacttgat cgatcttttc ttgctccaat ttcttatcct gtaaaaaaga agtcagttct
      361 tttatcagtt tctctttggc cttactagca tctgaaaata attgatcaag tgtaaaaggt
      421 ttaccatctt ctgtcaaatg tacttttgcc agactagttt tttcagtctc ttcaactttt
      481 gaagaagtta aatcatagac ttgtttcatc acacttcggt tgacaatccc ttttaaagtc
      541 gaatcctgtt tctccgtata ataaaaaacc agattttctt tgtcttccag attttcctta
      601 atatcctttg tcatgatttc ttgtacagat gaaatcactt gctccccttg gagaggataa
      661 taggcaatta cttctgcttg tcccttacga aaatgatctt tctgatttcc ctcacttaat
      721 tgatcatctt tctctttttt gagagattca attctttgtt caaaactttg ctttgtatat
      781 actttgtatc caatcattga accaaggacg caaatactta ccgaaaagat agctaccagg
      841 gctattaaac cgattctctt tttatcgtgc tcaacacgtt ttgctctact tttatccata
      901 gagatatcat atcaaattca ggctataatt tcaattattt tagaggaaat agtatattaa
      961 aaagagggga actccctctt aaaatcttat tttactgctt ctttcaaagc atctaccttg
     1021 tccaaacgtt cccatggaag gtcaatatct gtacgtccca tgtgtccata agccgctgtt
     1081 tgacggtaaa ttgggcgctt gaggtccagc atttggataa ttcctgcagg gcgaaggtca
     1141 aagatttgac gggctgcttt ttcaagcttg ctttcagcta ctcttcctgt accaaaggta
     1201 tcgatacgaa cagagacagg ctgcgcaaca ccgatagcgt aagccaattg cacttctgct
     1261 ttcttagcaa gacctgctgc aacgatattc tttgcaatat agcgagccgc atatgacgca
     1321 gaacggtcca ccttagtcgc atctttacca gagaaagcac caccaccgtg acgggagtag
     1381 ccaccataag tatccacgat aatcttacga ccagtcaaac ctgagtcccc ttgaggtcca
     1441 ccgattacaa aacgaccagt aggattgatg aagaattttg tttgctcatc caagtaagaa
     1501 gctggaataa cctctttgat aaccttgtta atgacatcat tgtgaatttg ttcgttgctg
     1561 acttctggat cgtgctgagt tgaaataaca actgtgtcca cacgtactgg acggtcattt
     1621 tcatcatact caactgtaac ttgtgattta gcatctgggc gaagatagct aatttcccca
     1681 gacttacgaa gttctgccaa acgacgaact aatttatgac tgagtgagat tggcaatggc
     1741 atgagctctt ctgtttcatc cactgcaaag ccaaacatga gcccttggtc tccagcacca
     1801 attaagtcca gcggatcttg atctgcattt ccacgaacct ccaaggcttc gttaacacct
     1861 tgggcaatgt caggagattg ttccaccaaa gatggatgta ctcctacagt ctcagcagaa
     1921 aaaccatatt ctgtattagt gtagccaatc tctgcaatgg tatcacgaac cacacggtta
     1981 atatccacat aagcatttgt agatatttca ccaaaaacat ggactgaacc agtatataca
     2041 gctgtctcag cagcaacgtg cgcctctgga tctttagcta aaatagcatc caaaatcgca
     2101 tccgaaattt ggtctgcaat cttatctgga tgcccctcag atacagattc agacgtgaat
     2161 aatttacgtt ctgacataaa aatgtccccc cttaaaaata gtgttataga gttacaaagt
     2221 actgataggt attttcattt tctaagagtt acaacgcgta agaaataaaa acgatacttt
     2281 tttatttctg aagctagtaa ggcgcatagg gtgaccgcct tatagcggca accgtagaat
     2341 gacgaacgac ttttgagccg tcattctttg cgaattgcga ctcgcaagaa aaaatagaaa
     2401 tttctaccat cttatcggga ctatataact ttaccattat accattttta aactcaattt
     2461 gcagtctata atgtagtgtt tctgtataga tagttcgttc tcataaaaca aaaaggattt
     2521 aatcctgtta tatacagaat taaatcaaaa tctgaattgc ttttaatatt ttttatactc
     2581 tctagaaaca cctctataat acatactcag acctaatata gcaataaatt tatgaataat
     2641 agtatttgat tcgaataaaa tgttaatgac tatcccagtc attataaaac taattgcaat
     2701 tattaaacgc tgcttcattt atacctcaac tttctatttc gtagaaaaaa aattcagttc
     2761 ctatccaaaa gcctataata gctccaccat atcctcttaa agaaacaata ccttctcaaa
     2821 gcatgagagt tcaccttcaa tgctggatag cattgcttca tccattactt caaatcgttt
     2881 cacctttgta ttcatcacga atatttcctt acatttatat taatttttta tcttgttaca
     2941 aacttggtaa gtttttctgc tcatttttcc ttatctcgtt ataccaaaag atatcaatcc
     3001 ttgagctaga tttttactca attctccata tgtggcaatt tctttataaa atgtcgcaac
     3061 ctttgggctc attgatttgt gactatgaat cccactaaca gcctgctgac gaagagcttc
     3121 tgacaactcc atgacaactc tctgctcatt ttttgtattc tctaacagag ttttataacg
     3181 taataaaata tctcgttcat ttttatgaat ctctgtatct aaaatcaaat tgtaaacctt
     3241 attgagaatt tcttctgttc tcttatccat aaattatcct ctataacctg aaagacaata
     3301 gctcctaccc aatgatgata tccaaccatt agccatgaca tctttagtta actcaaaatc
     3361 acacttagtt acaatatccc cattctcaat ttggaaatta acagatgaaa cattgtccaa
     3421 acatgtaatg aggcgaaaaa gtatcgtcat cacaacctta taaaattccc ttttggttta
     3481 ttccttttaa acctatacaa attaatagta gagtggctat gatatcaaca atatcttgtg
     3541 actttagtaa agacaagact cctagagaaa taatgtaaaa tatactaatt acttttctcg
     3601 ttataaacaa aaactacctg ccgctagccc gcctgttgaa atcgcaaatg taacaccaac
     3661 aagaccagta aatgcagtta gacctatata aattgacata ccaactcata ctccacaagt
     3721 aggatttaat ccaccagagt actcttgtaa atcactgttt tttagaattg aaaactgact
     3781 cattgaacta ttcattgtcc atcctctctt tttagcaaac tcgcatagct gtatcactac
     3841 ctagaatcgc agcaccagca gctccagcgt agaaaaagtc tatttcagtc atccctgcca
     3901 tacctgaaat acaacccaaa tcatttttac ctccttcaac acaataatag aaagagcatc
     3961 aactttgagc acattaactt gttctattgt ttttgtatcc atgatgaata tctcgtatat
     4021 aatagaaatc ttttgtcaga ttataacctt gacaagttca gtatagcaaa acaaaatcga
     4081 tttttatcac aaagtattta catgttgcta actccactta tcttcaaatt taaatagaca
     4141 cattatcgga gttatcaaaa agtcttgaaa aagtccagaa tgattataat ctgattaccc
     4201 aaaatatgat ttagacataa ttagttaata tcattctgca ttactagttt aatttgatac
     4261 agtgcaaatc gaaaaattat tagcaataat aaaaagccat ttccaaaaaa caagaaatac
     4321 attgctaaat cactctttaa atagtataga aaccaaataa gcatccaaag tatgctcatt
     4381 tttataaaga tattacttac tatccattca atctttagca agagataatt aactatgttc
     4441 attataacaa tctccatatt tccatcctca tcatatctat agctcaatca ctttactact
     4501 atgtctcaaa ataatttatt attatatact ataattaaaa cgttaactca ttcttttttt
     4561 gaaatatgta attcgatatt attcgagcaa ataatgtata ctactgtgaa taacactaca
     4621 aatatatgag tataaaataa tacagatgac gacttataac catctataac gacaaaaata
     4681 attgataata gtaacagaaa aaatgtaggt gctaaaatct ttttaataaa ttgcttgtat
     4741 cgattaaagc atagttcaaa actaatgttt cctctcttct tattatgcgt taataagtga
     4801 atcagcaact caaaaaacac actgcctaca gaaagaacta aaacttcgct tattggaaca
     4861 tttagaacta ttccaatcgc acctattata aaaaaagata taaaaagagc taaaattagc
     4921 ctaaattcac cactttccat caactaaccc cttttctata aaaatctagc gatagcccat
     4981 atagctgtac aatattgtgc tcataaaaca cctccagccc aagttccagc tcttggtgca
     5041 accaaactac caatcattgc tcctgctaat gtgcaaactt cccctgcact agctgttcct
     5101 ccctcaatac tagccaacat ttcggtatcc aaaacatcaa attgaatcgt tgtctttgtt
     5161 tccatgggga atacctccat attaattttt attatttttg tcagattata accttgacaa
     5221 gttcagtata gcagaacaaa atcgattttt accacaaact atatacatgt tgctaactcc
     5281 acttatcttc aaattcaaat agacacatta tcggagttat caataagaaa aaatctaacc
     5341 aatttagata gttcataata taggatataa taccagccat aacgaaaagg tacaaagctg
     5401 tacgatatcc tctctttggg tttttatcag ccttagtgtt aaaaataatg tacacacaac
     5461 aaattgcaat gagtataagc tgaatcatag tttcatttaa catattagca cctcttcttt
     5521 ttttaaaact attttaaata agttgttgtt tttgattcca cgatgattac atcctgtata
     5581 atagatgttt tttatgagat tataatcttg acaagttcag tatagcagga aagaagaaat
     5641 ttttacctca aagtctttaa atgtactttt ttcatcacga aacgtatttt ttcatgattt
     5701 gtaacctttc gagattaaaa ataaaccatt ttaaaattta ttccacaaat cgctgctaga
     5761 tattgtaaag tcgtcactct agcttttttg cccagtttta ttgttatgac tatttcctag
     5821 ctcatttgta ggaactagct aaaaaataat gtatataaag tcataacaaa agagtccccc
     5881 ttaaaatatt ctatagaata tcctaagaat ggcgactctt ttaaagttct atttccaaga
     5941 tctgtgtaaa gacataattt ttacttgtgg ttttcaaaca tacatttgga taagatttta
     6001 aaattctccg aacattggcc aagcctatac cccgacctac tccttttgaa gaattgtcac
     6061 gcttgaatat atctctcaat tctatctctc tttctaaagt agaatttttt acaatcatga
     6121 ctaatttatt ccattctgta tcctcaagaa aacaaactgc tattcctcct tctgctgcgg
     6181 actctatagc attatctaac aagatagaag taattgttaa gaagtcaatt tgttccatat
     6241 tttttataga gaactgttca ctaatctcca gggagactgg aacagataag ttttgggcct
     6301 ccgataactt agataatagt aaactcttta gcgccaaatc attgatattt cttagatgtg
     6361 ctacatcaaa acgcttgcct tttaattttt caccagaatt tttcaaaacg ctatcataga
     6421 cttgagaaac caaattaata tctttttgat cgatacctat ttttaagctc gataaaatat
     6481 tagcataatc atgtctaaag cttctaattt cttgatacag cccctcaact tgttgactat
     6541 aattactaag attttgcaat tgaatttctt tttgtagaac gactttctcc tgtaattctt
     6601 gaagtaactt tctatccaat agattaacaa aacaaataaa caataaaaag taaactgctg
     6661 tcaataattc tttataatcc aacattccat cccaacttac tttatcaatg tagtttatat
     6721 aaggcggtaa gataaaataa gcaatcatta aaatatccgc agtaattaag ataaattttg
     6781 tctttctatc taagttcttt atttttaaat gactaaaatt atattgaaag atactaataa
     6841 ggaataacac aataatttta gctagtaaag agaaaatcat tattcctaga tttgtttcat
     6901 atatagtttg caatatggga atcttataaa ttacaaagta acctaaaaag tttttgatga
     6961 catcccaaaa tccaagtgga aatagtgagt aaaaaagtag taaagaaacc ttacttctta
     7021 attcaccaat ggacaaagta tatagtataa aaaaagtggg ataactcaat aatgacccgt
     7081 aaaaataaaa ttctgttgat ccaaatacaa aaacaaatag taatattgaa agatagaaca
     7141 gaataatgga tattccgatt tttttcagat ttaatctctc acctaaaact ctagcatgga
     7201 tacataacat tagaaaagta cctataatat ctagaaaaaa tatcatctaa atctccttag
     7261 tcatgaagtc tttcgacagc tttcatgagt gaacgtatct ttaacctaga gacaatacaa
     7321 gaggacccgt tttgaaaatg aactacccga ttttctttat caattgaaga aatattataa
     7381 ggatttacga taaacgatct atgacattga aataagcgtg gttcctgttc tatgatttcg
     7441 gataattgtc cataaaattc aactatgtct cgttttgaat agagtgtcaa tttatgggct
     7501 attgacgatg tctcaatata caataaatct ttaaaaggga cttgaatttg agctttagat
     7561 cctgtaaata taaaagagtc ctcagatact gttattcctt gattatcgct aacacttaaa
     7621 ataacatttt caattcgttt gataaataat ggttcaggta attctttatc aatgaaatca
     7681 atagctgata ctaaatattc aaaagataca ggcataaact cagaatgtga tgttacaaaa
     7741 gcaatcactg cttgagaatc aagttttcta atttctctag caacgtctaa tcctcttttg
     7801 tcagcatctt ttatttcaat atcaagtaaa aataattgat gatttcctct ctctgaaatg
     7861 tcctctaaaa gctgttgagg ttttccataa acctcaaaat gcctatactc aatcttatta
     7921 tccaccaaaa tttttttgac aacattttca attctagtct gttgtaaaaa attatcttct
     7981 agtataaaaa tattcactac ttcactccta tatatttttc cactgatttt ttaatattgt
     8041 aaagtgtctt ctagaacaag caatgtctga tactctatca tccaaaaaca taatcgttct
     8101 tgtctcatga ttaaatcctg ctattttatc tttattcaca agaaactttc tatggcatct
     8161 aataagttct tctgaacttt cctcttcgat tttagctagt gaggttgaag cttcaaaatt
     8221 cccttcggct gttacaaaaa gaacagcatg agcttttgta gggtgagttg taacataata
     8281 aattttagag agtgatacct ttactatagc tctatcttga tataaaatgt agtctttcat
     8341 atttagcctc cttctatcag tttaatctta tcagaatgat ggaaattaaa ttataaaaat
     8401 ctcaaatggt ttctatacta tctcaaatgg taaaaatttt tttaatttag acatttcatg
     8461 ataaaataga tactttttag tatctcaata atattaatct ttctttattt gataaactaa
     8521 tatctaatga aaggaaagct atgaaggcaa ttcaaatttt acactattct ttatttggat
     8581 atattttatc tctgtattta aaatattatt tctttaataa aacattcaaa aatacaaaaa
     8641 ttctacaagg gcataagatt aaccacaaat ataaatcttc tacttatttt atcataaaat
     8701 caaggtccct aacatctaaa aaaatgaaac tctatttaaa atctatccaa tttagttcaa
     8761 aaaaatcaga agttatcatc ataggcagtc aaattgatta tgatgagctt tatcgaaacc
     8821 actatagtgt gtttggtgtt atagacataa ctaataataa atcattaaaa tatattaaag
     8881 ataaaattca tttttacttg gaagagctct atgaatttaa gaaggataag agtgattaag
     8941 tctatgaaat aaagttccaa gtcttataac tttatattcc gttacatcat agaattttga
     9001 tttcttcctc cacatgctcc agtttgatag caaattttag tgccatctta aagcagtcat
     9061 attttgtcag tcttattttt gctgagttat tgagattgat tggaacctct actattttat
     9121 tgatatgagc tagtttatta tgtgactttc agtcacataa tagaaaaagt tggagctcag
     9181 tactccaact ttttctatta aaatgtttgg cgttttgctt tacgctctgc acgaccacgt
     9241 gcgcgatttt cagcgcgctt ggtcttgcgg cgtttttcat caaccgccca ttgaattttc
     9301 ttcttataac ctggtttgac ttttttcttt ttctttttaa ccaaaccaat catttcgata
     9361 tcaagtttat cttgtttttt ctcacggttg gcacgacgat cacggtcata agtatcttga
     9421 aattccccgt ctttgaccat tttaggagta aacttgattc ccaatttctc caactcacgg
     9481 atatccgagt catcactggg ttggtaaagg gtaatagctg tacctggtag gccatttctt
     9541 ccagtacgac caacacggtg aacaaagaaa gataaatctt gcggaatggc atcattgatg
     9601 acatggctga caccttcaat gtcaatccca cgcgctgcca agtctgttgc gacaatgtac
     9661 tcaaaatcca gatttttcac ctggttcatg atacgcttgc gttcacgagg ggcaatatct
     9721 ccatgaatct tcgctacctt caagccttga gcagtcagat atgaatgcaa ttcatcagca
     9781 cgcattttag tgttaacaaa aatcattgcc aaatacggtt gcatcaactg agttaactgg
     9841 taaatttgag cattcttgtc acgtcccttt gtagaaatca accaattatc aatggtatca
     9901 gaaatgaccg ttttggtctt gattttctcc ataacaggat ttgacaagta ttttttcaag
     9961 aatggttgca gtttttgtgg gatagtcgct gagaagacca tgaattgcaa gtcttttgga
    10021 aggcttccag caatcttatc aacagtttcc aagaatccca tatccaaggt catatctgcc
    10081 tcatcgacca caaaggtctt agccttgtga atagctaggt caccagactt aactaagtca
    10141 taaatacggc caggcgttcc gataa
//
