LOCUS       NZ_KV802684            10228 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptococcus sp. HMSC073F11 Scaffold45, whole genome shotgun
            sequence.
ACCESSION   NZ_KV802684
VERSION     NZ_KV802684.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptococcus sp. HMSC073F11
  ORGANISM  Streptococcus sp. HMSC073F11
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 172555)
  AUTHORS   Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R.,
            Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A.,
            Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) McDonnell Genome Institute, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 213x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001810785.1-RS_2024_11_05
            Annotation Date                   :: 11/05/2024 23:57:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,892
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 16 of 57
            Pseudo Genes (incomplete)         :: 34 of 57
            Pseudo Genes (internal stop)      :: 10 of 57
            Pseudo Genes (multiple problems)  :: 3 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:06:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 47013
            Orig. end                         :: 57241
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV802684.1.
            The WUSC is a large strain collection isolated from clinical
            samples. Each sample is associated with metadata, including source,
            isolation site, 16s rRNA, metabolic, and other phenotypic
            information. The goal is to sample an adequate number of important,
            yet minor species, further adding to the catelogue of sequenced
            bacterial genomes and improving the diversity of the genomes
            available to the public. WGS will be preformed on approximely 550
            isolates. Samples were selected based on RDP analysis at the genus
            level. This is a reference genomes for the Human Microbiome Project
            and the work was funded by the National Institutes of Health (NIH)
            grant U54 HG004968.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5228](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5228)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(193..1302)
                     /locus_tag="HMPREF2764_RS00895"
                     /old_locus_tag="HMPREF2764_00895"
     CDS             complement(193..1302)
                     /EC_number="3.2.1.-"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174278.1"
                     /locus_tag="HMPREF2764_RS00895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00895"
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="WP_398578118.1"
                     /transl_table=11
                     /translation="MIFSDYQQHRQVEVQAEKEVQVEKTETTIETSKGEENKVIEDRLA
                     TMTLEEKVGQLFWARVPSNHQIEDLQSYHLSGYILFGRDFEGRSLEDIKTLTKDYQAAA
                     KIPLLIGSDEEGGTVTRISSILETPFQSPMALYQQGGMEAVLSDTKQKAELLKSVGINA
                     GLFPVADLARNQSAFIYDRTIGQDVQTTASYVQQVVEELKESKVGSTLKHFPGYGDNGD
                     SHTAIIQDNRFLDELRQADFLPFQAGIDAGADSVLVSHNILSKIDTVPSSISPKITDLL
                     RKELHFKGVIMTDDFDMAGIADFVSQDEAAFQVILAGNDLILGSSYQTQIPYLLKKISS
                     GELTEERIDESVRRVLTWKYDLGLLGAAQ"
     gene            complement(1564..2199)
                     /gene="trmB"
                     /locus_tag="HMPREF2764_RS00900"
                     /old_locus_tag="HMPREF2764_00900"
     CDS             complement(1564..2199)
                     /EC_number="2.1.1.33"
                     /GO_function="GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="trmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /locus_tag="HMPREF2764_RS00900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00900"
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="WP_001266095.1"
                     /transl_table=11
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKGKWRDLFGNDNPIHVEV
                     GSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTDYF
                     EDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSL
                     VSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAQF"
     gene            complement(2196..2990)
                     /gene="ccrZ"
                     /locus_tag="HMPREF2764_RS00905"
                     /old_locus_tag="HMPREF2764_00905"
     CDS             complement(2196..2990)
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="HMPREF2764_RS00905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00905"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000362999.1"
                     /sec_met_domain="APH (E-value: 4.6e-19, bitscore: 62.8,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYIPEHQWKEWLTYYGYKYNQTVLNKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(3151..3762)
                     /locus_tag="HMPREF2764_RS00910"
                     /old_locus_tag="HMPREF2764_00910"
     CDS             complement(3151..3762)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="HMPREF2764_RS00910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00910"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_070526473.1"
                     /sec_met_domain="Abi (E-value: 1.4e-20, bitscore: 67.1,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHAFCFGFLVLLIVITVPYTINHGDFFQNESELIIVSLL
                     VTSMSVAYARKFEMISFGMLSKKELLLFIAIFLLSVLETLVYIHFFAVSSGAGVQHLEE
                     VSRGISLSLILTSSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNAWPLLYYL"
     gene            complement(3793..4041)
                     /gene="blpZ"
                     /locus_tag="HMPREF2764_RS00915"
                     /old_locus_tag="HMPREF2764_00915"
     CDS             complement(3793..4041)
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="HMPREF2764_RS00915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00915"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_049502002.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDWLTPYILVLASDTIAFNVFVLTFVSAVVFNS
                     LNSMLALMAIFLGAGYVVGFWLLKWFVLERLELKDDL"
     CDS             complement(4100..>4400)
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /locus_tag="HMPREF2764_RS00920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00920"
                     /product="immunity protein"
                     /protein_id="WP_261673647.1"
                     /transl_table=11
                     /translation="FLNFSLVFQFLLVVINLASILLTVYLTREMRIREFEDDDLVSPRT
                     NQLMYIGLTGFMSIICLYRGVTAGESYQQLIAYIGAILCLIIMLLLIWGLKYYKK"
     gene            complement(4100..>4404)
                     /locus_tag="HMPREF2764_RS00920"
                     /old_locus_tag="HMPREF2764_00920"
     assembly_gap    4405..4504
                     /estimated_length=unknown
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4505..4637)
                     /locus_tag="HMPREF2764_RS09470"
                     /old_locus_tag="HMPREF2764_00925"
                     /pseudo=""
     CDS             complement(<4505..4637)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257557.1"
                     /locus_tag="HMPREF2764_RS09470"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00925"
                     /product="immunity protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKKIFFKKQTIFLARLFLGQLPLLVSTYLFLSRQFLNFSLVFQF"
     misc_feature    complement(4613..4626)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.67"
                     /tool="antismash"
     gene            complement(4666..4986)
                     /locus_tag="HMPREF2764_RS00925"
                     /old_locus_tag="HMPREF2764_00930"
     CDS             complement(4666..4986)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645121.1"
                     /locus_tag="HMPREF2764_RS00925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00930"
                     /product="hypothetical protein"
                     /protein_id="WP_000645118.1"
                     /transl_table=11
                     /translation="MKAKYGNQIVDVWEIGQATPKPSWVEEAFQKNYMVWLDNHVRILM
                     AGLNTSLATNIKFGLVGTVGGGFAGYGMYVLGYPGDYIDITNHRVVSAKTFHKSYQILE
                     ND"
     gene            complement(5001..5228)
                     /locus_tag="HMPREF2764_RS00930"
                     /old_locus_tag="HMPREF2764_00935"
     CDS             complement(5001..5228)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093254.1"
                     /locus_tag="HMPREF2764_RS00930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00935"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_001093489.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.1e-16,
                     bitscore: 52.7, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKTMSQFEMMDTEMLAKVEGGFGGWGDMVAGLLGGLAPSPTLD
                     QLNGKWPIIHFSKPCGPYGIGGTPNSCNGI"
     gene            complement(5249..5653)
                     /locus_tag="HMPREF2764_RS00935"
                     /old_locus_tag="HMPREF2764_00940"
     CDS             complement(5249..5653)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /locus_tag="HMPREF2764_RS00935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00940"
                     /product="immunity protein"
                     /protein_id="WP_070526476.1"
                     /transl_table=11
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSLVFQFL
                     LVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGVTAGE
                     SYQQLIAYIGAILCLIIMLLLIWGLKYYKK"
     gene            complement(6095..6214)
                     /locus_tag="HMPREF2764_RS10225"
                     /old_locus_tag="HMPREF2764_00950"
     CDS             complement(6095..6214)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042929.1"
                     /locus_tag="HMPREF2764_RS10225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00950"
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_004269601.1"
                     /transl_table=11
                     /translation="MDFKSFLIVFVVGMFVSFITSLIREKFLKSPKKNKDRSN"
     misc_feature    complement(6765..6774)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.78"
                     /tool="antismash"
     gene            complement(6931..8282)
                     /locus_tag="HMPREF2764_RS00950"
                     /old_locus_tag="HMPREF2764_00955"
                     /pseudo=""
     CDS             complement(6931..8282)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257533.1"
                     /locus_tag="HMPREF2764_RS00950"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00955"
                     /product="sensor histidine kinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEIVWKIVYAFLIAGLELFIFFKVDGIVLTLERFFKAFLFKLLLA
                     VVFVTINYIVGNTYLSYFTEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRGIS
                     YFVLPFLDQGQINDNYSFIWVFIMIFNFFISLAFLKWLDYDFTGLRKEILDKAFQKSLT
                     KINWIMGAYFLVMENLSYFEYAYDIQSKTVRHLILVFYLLFFMGIIKKLDTYLKDKLHE
                     RLDQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIKEVYDS
                     VLKDSSQKLQDNKYDLGRLVNIRDRALKSLLAGKFLKARDKKIVFNVEVPEGRFR"
     gene            complement(8296..9033)
                     /locus_tag="HMPREF2764_RS00955"
                     /old_locus_tag="HMPREF2764_00960"
     CDS             complement(8296..9033)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /locus_tag="HMPREF2764_RS00955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00960"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_070526483.1"
                     /transl_table=11
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHQIVPSSFEVFGKPDQLLA
                     EVHEKGSHQLFFLDIEIRNEEMKGLEVARRIRDRDPYALIVFVTTHSEFMPLSFRYQVS
                     ALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYYLET
                     SPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKLLYFP
                     NGGSCMIARYKVREVSEAINNLH"
     misc_feature    complement(9177..9190)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.71"
                     /tool="antismash"
     gene            9336..9647
                     /locus_tag="HMPREF2764_RS00960"
                     /old_locus_tag="HMPREF2764_00965"
     CDS             9336..9647
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399208.1"
                     /locus_tag="HMPREF2764_RS00960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF2764_00965"
                     /product="helicase BlpT"
                     /protein_id="WP_070526485.1"
                     /transl_table=11
                     /translation="MQTAQTLITDLNKAYQVCKQATADDVRFQEQLNSILGFLAKAETV
                     DNRVLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLSLYGPTILL"
ORIGIN
        1 gcgcttctat gacaggttct acaacttctc tgactaattc tacgattgtt gcgattgcgt
       61 ccacctcctc ataagcaaga ggcgaagata tttccccgcc tctctttctc atattcttgc
      121 tataagtata acacaaatca agctaacctg caaggatttg accttgaagc tttttgtctg
      181 ggcttacaag acttactgcg ccgctcctaa taatcctaag tcgtatttcc aagttaaaac
      241 acgtctcaca gactcatcaa tacgttcttc tgtcaattct ccagaggaaa ttttctttaa
      301 taaataaggg atctgggttt gataggaaga ccctagaatc agatcatttc cagctaaaat
      361 cacttgaaaa gcagcctcgt cctgactgac aaaatcagct attcctgcca tatcaaaatc
      421 atccgtcata atgacacctt tgaaatgcag ttccttccga agaaggtcag tgattttcgg
      481 tgagatagat gatggcactg tgtcaatttt agataagata ttgtgagaga ctaagacact
      541 atccgcccct gcatctatcc cagcttgaaa gggaagaaaa tctgcttgtc tcaattcatc
      601 taaaaaacgg ttgtcttgga taatagcggt atgactatct ccattatcgc catatcctgg
      661 aaaatgtttc aaggttgatc caaccttact ctctttaagt tcttctacga cctgctgaac
      721 atagcttgca gttgtttgga catcttgtcc aatcgttcga tcataaatga aggctgactg
      781 attccttgca agatctgcaa ctgggaaaag accagcattg atcccaacag attttaacag
      841 ttctgccttt tgcttcgtat cagaaagcac cgcctccatt cccccttgct gataaagtgc
      901 cataggagat tggaaaggag tttctaaaat cgaactgata cgagttactg tccctccttc
      961 ttcatctgaa ccaatcaaca aaggaatctt ggcagcagct tggtagtctt ttgtcaaagt
     1021 ctttatatcc tctaaacttc gcccctcaaa atctcttcca aataagatat aaccagacaa
     1081 atgataggat tgaagatctt ctatctgatg attagaagga actctggccc aaaataattg
     1141 tcctactttc tcctccaagg tcatcgtggc caatctgtct tcgataacct tattttcctc
     1201 tcctttactg gtttcaatag ttgtttcagt tttttctact tgaacctctt tttctgcttg
     1261 cacctctact tgtctatgtt gttggtaatc agaaaaaatc aaaccaacta tcacgttgtt
     1321 ggtaatcaga aaaaatcaaa ccaactatca caaggaaaaa gaaaagatag aatacaagag
     1381 ggtttatact atttctcata acctttccat tataccactt atctggccgc aaatacacac
     1441 ttagtaaaaa acacctgttt tgaaatcaaa ctaaaaggac ttaattctgt gcaatacaga
     1501 actaaatcct tagaatatat gataactttt tgaatgcgaa acagtttttg aaatctcttt
     1561 agattaaaac tgagcctcaa ctcggtagat aacttgaccc ttattggaga atttttgctc
     1621 gtattctgtc atgacattgc cttcaaaatc actggcatgt aaatcaagcc agacaccatt
     1681 gagtttcatg ccatactgag aaaaactcac taggctatac tcaaacaagc cacggttatc
     1741 cgtcttaaaa tgaatttctc cattttcagg caagatacgt ttgaaggtat ccaagaaggt
     1801 cttgtaggtc aaacgacgct tttcatggcg ttttttaggc caaggatctg aaaagttcag
     1861 atacaagcga tcaatctcac cgtcttcaaa gtagtcggtc aagtcagaac catccaccca
     1921 caagagcttg atgttaggca ctccaacttc aagcaccttg tccaaagcgt agctcaaaac
     1981 agacttttga atatcaatcc cgatatagtt gatgtcaggg ttttgcttgg ccatacctga
     2041 aacaaaggca ccctttccac ttccaacttc cacatgaatg ggattatcat tgccaaacaa
     2101 gtcccgccat tttcccttgg cttccaaggg attgaggact acatactggg gatttgcctc
     2161 tagtaattct gtcgcccctt tacgatttct aactctcatc ttctctttcc atacttgtct
     2221 cggaagtgac gcaagccatg gatctcccga ttgacatttt ctaaatcttg gttcatataa
     2281 tacttggaaa tctggctcaa ataagacaat tgaccgtacc aatacaattt atttaatacc
     2341 gtttgattat acttgtaacc gtaataggtc aaccattcct tccactgatg ttctggaata
     2401 taatggcaga gcatatgggc cacatcaaac atgcggtcgg tcaaacgaac cgaatcccaa
     2461 tccaccaaat aaatcaagcc actgtctgtc tcaatccaat tactatgtcg tacatctcca
     2521 tggacaatgg tcgcatggtc ctctctaaat cctggaatag tctgacgtaa atcagccatc
     2581 acttcactga taaaatgatt tttacgcaaa gcatctggag ccgtttcctg ccaagactgt
     2641 agtaaatcta caggtgtttc catggcatac cccaaacgac tcaactgtgt catcaacgga
     2701 cgtgagcgat gcagacgggt taagatattg acgatttgtt tacgattcat atcatagggg
     2761 gtcaatatct tgcctgtcaa ccattcttga gcacacatat cacgcccatc tgccaaacgg
     2821 cgactccata ataattgtgg agcaatttgt tctctagcta gaccaggtag gattggagag
     2881 gtgttcattt ttacaaagat gcgcttccca tcaggatagc tacccatata agctttgcca
     2941 cttttcccag gtatgggagt cagtgttagc tcattatcac ccaaatccat ttctttcctc
     3001 cgtataaagt ttccttacta ttctactagt ttttggccta ttcgtcaacc gctctaaccc
     3061 gattctcaaa gaaaagccta tggattggat tgggtatcat tatagaaaga aaaaggcaag
     3121 caccgtcttc tgcttacctt ttcgtgattt ttataaataa tataagagtg gccaagcatt
     3181 gtaaaacatg tgaactagag tagaaaccca tagattttgg ctctttttat aggcaaagcc
     3241 cagcaacaag cccccaagta gataaaaaat aaacgaaggg acattagaag gtccatgcat
     3301 aaaagaaaag agagaggaag ttagcacaag ccctaaccaa gaattgtcaa aaacggcacc
     3361 ttgaagaagt cctctgaaaa tgagttcctc ctggatggga ccaaaaactg aggaagtcaa
     3421 aatcaaagac agagaaattc ctctactgac ttcctccaag tgttgaactc ctgcgccaga
     3481 agaaacagcg aagaaatgaa tataaaccag cgtctcaagt acacttagaa gaaaaattgc
     3541 aataaaaagc aaaagttctt tcttgcttaa catcccaaaa gaaatcattt caaactttct
     3601 agcataagca acactcatcg aggttactag aagacttaca ataatcaatt cagattcatt
     3661 ttgaaaaaaa tccccatggt taatcgtata aggaactgta atgacgatta gtagtactaa
     3721 aaatccaaaa caaaaggcat gaaatttatc aaaaaactcc ataaaactag cctcctcaac
     3781 aaacaaactt ccctacaagt catcctttaa ctctaacctt tccaaaacaa accattttag
     3841 taaccaaaat ccgaccacat agccagcccc taagaagata gccattaaag ctagcatgga
     3901 atttagggaa ttaaagacca ccgcagatac aaaggttagc acaaaaacat taaaggcaat
     3961 ggtgtcagaa gccaaaacca gaatataagg cgtcaaccag tctaaggttt tggaatctag
     4021 gaaaaataag tgtttataca tgatgacctc ctcctgccag tctttagact agtcccagat
     4081 taagaagaaa acccttcctc tactttttat aatacttcaa accccaaatg agcagaagca
     4141 tgataatcaa gcagagaata gcgccaatat aggcaattag ttgttggtaa gattctcctg
     4201 ctgtgacacc tctatacaaa caaataatag acataaaccc tgtcaagcct atatacatga
     4261 gttgattggt tctaggacta accaaatcat catcttcaaa ttctcttatc ctcatttccc
     4321 tagtgaggta aacagtgagc aaaatggaag ctaagttaat aaccactaag agaaattgga
     4381 aaaccaagga aaaatttaaa aactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnngaaatt ggaaaaccaa ggaaaaattt aaaaactggc gagatagaaa tagataagta
     4561 gagacaagta aaggcaactg acctaagaac aatctcgcaa ggaaaatggt ctgtttttta
     4621 aaaaatattt ttttcattgc tttctccatt acaattaaat gacatctaat cattttctaa
     4681 aatttgataa cttttatgaa atgttttagc agaaacaact ctatgattgg tgatatctat
     4741 ataatcacct ggataaccaa gaacatacat cccataacca gcgaatcctc ctccaactgt
     4801 tccaactaga ccaaacttga tattagttgc aagagaagtg ttaagacctg ccattagaat
     4861 gcgcacatga ttatccaacc aaaccatata atttttttga aaagcttcct ctacccaact
     4921 tggtttagga gttgcttgac caatttccca aacatcaaca atttgattcc catattttgc
     4981 cttcatagct tcaatccttt ttaaatacca ttacatgagt ttggagttcc ccctatacca
     5041 taaggaccac atggttttga aaaatgtata ataggccact taccatttaa ttgatccaaa
     5101 gttggagaag gtgctagccc acccaataaa ccagcaacca tatctcccca acctccgaaa
     5161 cctccttcaa ctttcgcaag catctcagta tccatcattt caaattgtga catcgttttt
     5221 gtattcataa caaaaacttc attagcccct actttttata atacttcaag ccccaaatga
     5281 gtagaagcat aataatcaag cagagaatag cgccaatata ggcaattagt tgttggtaag
     5341 attctcctgc tgtgacacct ctatacaaac aaataataga cataaaacct gtcaaaccaa
     5401 tgaacataag ttgatttgtt ctaggactaa ccaaatcatc atcttcaaac tctcttatcc
     5461 tcatctccct agtgaggtaa acagtaacta aaattgaagc caagttaata accactaaaa
     5521 gaaattggaa aaccaaggaa aaatttaaaa actgacgaga tagaaataga taagtagaga
     5581 caagcaaggg caactgacct aggaacaatc ttgcaagaaa gatgttccgt tttttagcaa
     5641 gaaaagtttt catttctttt ctcctttctt tttcatgata gccaaataaa tcatcactgc
     5701 aatcacatag gctatggtat agaatagctg ataccaagca ctctccctaa gcggatatag
     5761 aaagatggac atgatcagat acaagatgaa aatgacaaat attttttctt cataagattt
     5821 cctcctgaat gtttgttgag ctagaacctt tacactgcta gtatagcact cattttgacc
     5881 ttggatcact gaaatcataa atggtcatca aaacatcttg aattgttaaa aatttaaaaa
     5941 agcaagcatg aaaaacatac ttgcccttta caccatattg ataccaactt aatttgtaga
     6001 ttttttatcc tgctatcaca tatcattttg acaggcgaaa caatattaaa gaaactccac
     6061 tgtagattaa actagcgata aaaaatcttt ttatctaatt agatctatct ttattcttct
     6121 ttggagattt taaaaatttt tccctaatta aagaagtaat aaaagaaaca aacatgccaa
     6181 caacaaaaac aataagaaaa cttttgaaat ccataattac ctccaagtac agttcctcta
     6241 cttacaggct caagccttta agcaagcata atagccaatg gtaacattgt ttttgcattc
     6301 acgaggaata ctctttttat tttcgtccta ttgcaacctt aataaggtta gtatactact
     6361 attttttaaa attttccatc caaatcacaa atggtcatca aaacatcttg aattgttaaa
     6421 aaatttaaaa aagcaagcat gaaaaacatg cttgccctgg gggaacttga caatgtgaaa
     6481 atacaaaata gaaaactact caccctcact tccgtatgtt ctcatatatt ctcatgatcg
     6541 caacaaggat agcagctgtc ccacttgcca aagcgataag ggtaatgata atgttgagga
     6601 tttcaggcgt actccctatt ctgttgataa gacggagtag ggatataact gtcaactgaa
     6661 tcaggagtat tgattatact cttaccattg aagtaagtct gttttcaact gcatataaaa
     6721 aggctggtag caaaacacag actatagcaa tcacaaacag attgctcccc gttccttctc
     6781 ccttgttcac cacggaaatc atgagaagat ttgatgctat aatcaacgta gaacagatga
     6841 taaaatagga tttcttattc atttaatata cctcgcatac tatagtattt taattgatga
     6901 ctacaggcag tacttctttg cacaagtctt ctaatcatca actgacatcg agtgaactgt
     6961 taaaacctga cgaaagactt gattttggca ggtggtattt agactggtat taggatgact
     7021 ttccacaatc ttcatgacgg tatagagacc aactcctctc tcctcccctt tagagcttgc
     7081 tccaaaggag aagatttcag aaatatctat gccctcttct ttgatggagt tttcgatgat
     7141 aaaggtctcc tgtgccccat tttttaaaaa ggcgattgaa acatgaggtt gactggcctc
     7201 tgcactggct tcaatagcgt tgtcacaaag gatagacaca atggttagaa aatcaagcaa
     7261 gctcatcccc tctacctgaa tctcccctca ggaacttcga cattaaagac aatcttctta
     7321 tctctggctt ttagaaattt ccctgctaga agacttttga gggcacggtc acgaatattc
     7381 accaatcggc ctaggtcata tttgttgtcc tgcaatttct gactagaatc ctttaagacc
     7441 gagtcgtaga cctcttttat ctgctccata tcctcctctt caattcccag acgtaagctg
     7501 gttaagaggt tggtgtagtc atggcgaaaa ctccgcactt ccttgtaaag ttcctctata
     7561 tgccgactat agcgttccat atctctgtag cgcaaggcct gctcttggtc caatctctca
     7621 tggagtttgt ccttcaaata ggtatccaat ttcttaataa ttcccataaa aaagagcaag
     7681 taaaagacta ggatgagatg gcgaacagtc tttgattgga tatcgtatgc atattcaaag
     7741 taagacagat tttccatgac tagaaagtag gcccccatta tccagttaat cttagtcagg
     7801 gacttttgaa aagctttatc gagaatctcc tttctcaaac cagtaaaatc atagtccaac
     7861 catttcaaaa aggctagaga aatgaagaaa ttgaaaatca ttataaacac ccaaataaat
     7921 gagtaattgt catttatttg cccttggtct aaaaacggaa gcacaaaata agaaatacct
     7981 ctataaaaga gattcaccaa tatcattgga aagagaccat aaaagaaaag gagtttttta
     8041 ggaaggcctc ttaacaataa gaaagacaag cctataccgt acaagggttc cgtaaaataa
     8101 gataggtaag tatttcctac tatatagtta atcgttacaa aaacgactgc taaaagtaac
     8161 ttaaaaagaa aagctttaaa aaatctctcc agagttagaa caattccatc caccttaaaa
     8221 aatataaaca attccaatcc agctatcaaa aatgcatata ctatcttcca aactatttcc
     8281 attacattac ctcacttagt gtaagttatt gatagcttca gacacttctc tgaccttata
     8341 acgcgcaatc atacagcttc cgccattggg aaagtaaagc agtttttctt tcttatccaa
     8401 atgcaccaca tttgcaggat tgatgagaaa agagcgatgg cactgcaaga gacggggttc
     8461 ctgcttaaaa acctcctcta aactcgctgt aaattccagc ctgtccgtct tggtatagag
     8521 aataacacga tgggctctgg gcgatgtttc gagatagtag acctctttaa aaggatactg
     8581 gaattgagca aattttgatt taaaataaaa gcaatcttca gctagacttt tactatcttg
     8641 actattggca tagaggaggg ctgtctcgat acgagattca aactcctccg ccgataatgc
     8701 cttatcaatg tagtccaaag cagacacttg gtagcgaaag gacaggggca taaactccga
     8761 gtgagtcgtc acgaagacaa tcaatgcata aggatcccga tcccgaatcc ttctagccac
     8821 ttccagaccc ttcatctcct catttcgaat ctcaatgtcc aaaaagaata gctgatggga
     8881 gcccttctca tgcacctctg ccagcagctg gtctggctta ccaaaaactt caaaagagct
     8941 gggaacgatc tgatgttctt tcaagagttt ctcaatcgtc gtttcaatcc tagtctgttg
     9001 ggaaaaatca tcttctaaaa caaatattct catcttctta ctccccttgt ttcaatttct
     9061 tcctaaaaag agaatagcaa aaatactatg atacgagccc accaaatcct aaacaggcct
     9121 tcaacgctcc tgaagggtag cttgttcctc aaataagcat gataatctta ccactatttt
     9181 atcataaaat cttaacagta ccattcagga aatttcaagg acaattaaag atttgatggt
     9241 gaaaaaggag ataaaagtga taaactgaca atatcaaaac acagttgcta gaaacaagac
     9301 tagtacccag attagaggag gaattctcat gacagataca gaccgctcag actttgatta
     9361 ctgacttaaa taaagcctac caagtatgca aacaggcaac cgctgacgac gtccgctttc
     9421 aggagcaatt aaactctatt cttggttttc tagccaaggc tgagacagtg gataatcgag
     9481 tcttgattga actggaaaaa ttttaccaga cttccagtct tctcatggga ctcagcgctc
     9541 ttgatccaga tgctccaact cgcgccgctt ggcgggccta tgaccgtttc cactttgacc
     9601 aagtcaagac caagttgagc ctctacgggc caaccattct cttgtagaaa ataaaagaag
     9661 ctgagacaga ctgaactcaa cttctttttt agtgtcatat aggcaatgtt agtcctgcat
     9721 ctgacgtttt acctgataag gtaaatacaa gactagtaaa accaaaccag ctcccagtaa
     9781 ggctaaaagg gctagttttt cttggaaggt aatcaatccg acaactaatt ccacaagtaa
     9841 aacaaaactg gtcaatcctc ggactaccgc ctgcaagcct ttttcctttt gccccttgtc
     9901 cagcggaaag agttgggtca aatactggta gtcaaaggca tgatagaggg ccagcaactg
     9961 gaagagcaag aggtagttaa agagaaccac cactgctgtc gcaatccaag cttgctcgat
    10021 aaagacctgc gccagtatgg aaagcaagag cagacgaaga ctgagcgaaa agaggtctcc
    10081 atttcgcaga taagaacgca gatagagatt ttgccaaatc ttcccaggca ccttctgaac
    10141 agcctttaag ataaagtcca gataggcacg acgtttgaca ctgtttgaaa tccccttgac
    10201 ctgggtaaag agggcaaaga aacgaagc
//
