LOCUS       NZ_CP014859            11023 bp    DNA     circular CON 20-NOV-2024
DEFINITION  Actinoalloteichus hymeniacidonis strain HPA177 chromosome, complete
            genome.
ACCESSION   NZ_CP014859
VERSION     NZ_CP014859.1
KEYWORDS    RefSeq.
SOURCE      Actinoalloteichus hymeniacidonis
  ORGANISM  Actinoalloteichus hymeniacidonis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Actinoalloteichus.
REFERENCE   1  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J.,
            Zotchev,S.B. and Ruckert,C.
  TITLE     Complete genome sequence of the actinomycete Actinoalloteichus
            hymeniacidonis type strain HPA 177T isolated from a marine sponge
  JOURNAL   Stand Genomic Sci 11, 91 (2016)
   PUBMED   28031775
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J., Zotchev,S.
            and Ruckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 159x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001747425.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 01:17:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,434
            CDSs (total)                      :: 5,374
            Genes (coding)                    :: 5,337
            CDSs (with protein)               :: 5,337
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 3 of 37
            Pseudo Genes (incomplete)         :: 34 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:46:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5642540
            Orig. end                         :: 5653563
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11023
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="25"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11023
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11023
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6023](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6023
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            62..1174
                     /locus_tag="TL08_RS23740"
                     /old_locus_tag="TL08_24070"
     CDS             62..1174
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.5"
                     /locus_tag="TL08_RS23740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24070"
                     /product="sensor histidine kinase"
                     /protein_id="WP_084643393.1"
                     /transl_table=11
                     /translation="MSNSTYALPPAASIQPPPANRDMQQRIDIVDEFEFRLGVSEISRT
                     TDPGFWPQVRAQVSMILDDCLAKISDPDFVPSQETRAAANDFGSEVAKRSVRPNDVLDA
                     GSVLFELVSESFLQDAGPTAAPRRLVEMLRILERTISEHVTAILDGYERNLLERAAHSK
                     AEDWRRLARDIHDWVGNSVSLVARNLDLYQLEQSRGDEPSTDRIVRARAAVDDVLEATR
                     RVISDLRHQEPATCISHALSRFVEAAKPERTRSHIVVRGEEAQLPLDHRDALFVMLREC
                     LRNTIRHADAENVAVSVDIEPKSVRAIVIDDGVGFDVDAVHRAGLAYGLDSMRERAALL
                     GGKATITSAPDSGTTVEISLPLGVVDGCRN"
     misc_feature    83..85
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1158..1811
                     /locus_tag="TL08_RS23745"
                     /old_locus_tag="TL08_24075"
     CDS             1158..1811
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="TL08_RS23745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24075"
                     /product="response regulator transcription factor"
                     /protein_id="WP_069852265.1"
                     /transl_table=11
                     /translation="MAAATDTIRILLVDDHTLLREAVRDVLLTEDDFTVVGEAGDGATA
                     VGLAATTKPHVVLLDVEMPRSKPLETVERLLQVAPKARVIVLSMYDDPQLVREMLGLGI
                     AGYLHKSIGQRDLINAIRSAGTGEGQVTVSVSREALALSGANAEVSGPLSSREIEVLAL
                     VSEAMSNRQIAGKLSITEGTVKRHLRNIFAKLGAMSRIDAVNKAVDLALIRKRY"
     gene            complement(2075..3196)
                     /locus_tag="TL08_RS23750"
                     /old_locus_tag="TL08_24080"
     CDS             complement(2075..3196)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FabF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012337.5"
                     /locus_tag="TL08_RS23750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24080"
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_069854088.1"
                     /sec_met_domain="FabF (E-value: 3.2e-20, bitscore: 70.6,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.4e-13, bitscore:
                     49.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAASTVEPLTITACSAVSAAGFGIEVLGDRLRTADRATASAVEG
                     TSAERSTEEDFPPRPVRTIADLRVAEFLGRRGTRNLDRLTLLGLITAKQLLDETGPVAE
                     DDQPDVGVAIGTAAGSLRTQAEIGRDTLIEEKPYLVNPSRFPNTVMNSCAGQIAIRNSL
                     QGVNATLAAGQLSSLHALRYARVAIGRKRAGRLLVGGVEELSPQLAWGWHRTSVVPSDV
                     PLGEGAALLMVSEVEQDRDSAIAQLLACEVGFAAPGSRTIPTRVLRTLLDRALTRSGLT
                     PDEVDVVSLGAVGSPTLRRVEERAVTEVLGERAERIRVADVVGECFSASGALQVAALLA
                     LWRREPAERPRVGVVTSVGPDGNLGCAVIREAA"
     gene            complement(3196..4419)
                     /locus_tag="TL08_RS23755"
                     /old_locus_tag="TL08_24085"
     CDS             complement(3196..4419)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (80-403). E-value:
                     2.2e-59. Score: 193.4. Matches aSDomain:
                     nrpspksdomains_TL08_RS23755_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662516.1"
                     /locus_tag="TL08_RS23755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24085"
                     /product="beta-ketoacyl synthase"
                     /protein_id="WP_069852267.1"
                     /sec_met_domain="t2fas (E-value: 2.1e-102, bitscore: 341.2,
                     seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.7e-43, bitscore:
                     147.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEPMPRRVVVTGLGAVSSIGTGADVFAAGIRAGRNGFRPINSFD
                     STGFPATIAAEVPDFDPTALIRNLDVEQWGRSSLLAAAAARQAVGDSGLDPDELAASAA
                     GSIIGTTGGESVPIGQLVGQWVADGLKSIDGRIVPQALASQIANAVNSELGLTGDAVTL
                     GTACSASNFAIGYAYDLVRTGEADFMIAGGADAVNRSTHAGFYRLGALSTDGVCRPFDV
                     DRSGIITGEGGVALLLEPLEHAQARGARIYAEVLGYGMNCDARHMVNPDSERIAECIRL
                     AHDSAGVTPEDIDYICAHGTGTATNDSTEVSAARKVFGETLPPISSIKSSIGHTMGAAS
                     GFGALICCLAVHEGFLPPTANLQRIDPALGRGIDPLPGVAREEPVRIAQNHGFAFGGNN
                     AITIFGRL"
     aSDomain        complement(3211..4179)
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TL08_RS23755_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.20E-59"
                     /label="TL08_RS23755_PKS_KS.1"
                     /locus_tag="TL08_RS23755"
                     /protein_end="403"
                     /protein_start="80"
                     /score="193.4"
                     /tool="antismash"
                     /translation="AAAAARQAVGDSGLDPDELAASAAGSIIGTTGGESVPIGQLVGQW
                     VADGLKSIDGRIVPQALASQIANAVNSELGLTGDAVTLGTACSASNFAIGYAYDLVRTG
                     EADFMIAGGADAVNRSTHAGFYRLGALSTDGVCRPFDVDRSGIITGEGGVALLLEPLEH
                     AQARGARIYAEVLGYGMNCDARHMVNPDSERIAECIRLAHDSAGVTPEDIDYICAHGTG
                     TATNDSTEVSAARKVFGETLPPISSIKSSIGHTMGAASGFGALICCLAVHEGFLPPTAN
                     LQRIDPALGRGIDPLPGVAREEPVRIAQNHGFAFGGNNAITI"
     gene            complement(4412..4681)
                     /locus_tag="TL08_RS23760"
                     /old_locus_tag="TL08_24090"
     CDS             complement(4412..4681)
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (10-72). E-value: 3.8e-14.
                     Score: 44.7. Matches aSDomain:
                     nrpspksdomains_TL08_RS23760_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.5"
                     /locus_tag="TL08_RS23760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24090"
                     /product="acyl carrier protein"
                     /protein_id="WP_069852268.1"
                     /sec_met_domain="PP-binding (E-value: 4.5e-12, bitscore:
                     44.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQGIADGVRDKIREIIANELELDPSELTESGHLVDDFDADSLSL
                     ITVVARIEKELGVVIPRDELPNLSTLGLVFSAVDRYGIGQADNA"
     aSDomain        complement(4466..4651)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TL08_RS23760_PP-binding.1"
                     /evalue="3.80E-14"
                     /label="TL08_RS23760_PP-binding.1"
                     /locus_tag="TL08_RS23760"
                     /protein_end="72"
                     /protein_start="10"
                     /score="44.7"
                     /tool="antismash"
                     /translation="DKIREIIANELELDPSELTESGHLVDDFDADSLSLITVVARIEKE
                     LGVVIPRDELPNLSTLG"
     misc_feature    complement(4619..4621)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4849..4862
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.28"
                     /tool="antismash"
     misc_feature    4849..4864
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.99"
                     /tool="antismash"
     misc_feature    complement(4850..4863)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.4"
                     /tool="antismash"
     gene            5001..6023
                     /locus_tag="TL08_RS23765"
                     /old_locus_tag="TL08_24095"
     CDS             5001..6023
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.5"
                     /locus_tag="TL08_RS23765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24095"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_172803865.1"
                     /sec_met_domain="AfsA (E-value: 3.8e-16, bitscore: 57.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPVLSTSTAPPGLDAAELSFTKTIDRGLVHRSSVSEVFVTDLH
                     QAGELAFAAAAQLPLTHSYYNDHVARPAMVDPLLIVECARQAGIYGAHLSGVPMRTAML
                     VNSFTLRLDDPTELIMSRRPVELRIDSVMAATRVRGGVLRQGHAEQRFTIGGRPVGSQV
                     MEIQMLSHHEHDALRHLQRGNPAPSTADMTGGPYPNSVAPGLVGRVHPMNVVLADVRYG
                     ADEVRAVMAPWYENRALFDHDYDHLPAMTLTEGARQLALLSVRESGATEAAGFPPVVGI
                     AATFHRFAELDAPVHATTATEPAAEAGALTRTVVFTQDGITIATISISLAMPASIGERR
                     "
     gene            6020..6685
                     /locus_tag="TL08_RS23770"
                     /old_locus_tag="TL08_24100"
     CDS             6020..6685
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012905.5"
                     /locus_tag="TL08_RS23770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24100"
                     /product="HAD family phosphatase"
                     /protein_id="WP_069852272.1"
                     /sec_met_domain="HAD_2 (E-value: 1.4e-05, bitscore: 23.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEPTRTVDAVWTDFGGVLTPPVSETMAAFCRKKNLDPAPLGRAI
                     WKVTQSYGTTDIMEPLDTPLVSERDWLRQVTDAMFEDTGIRVEITSMGEDWFDGRAVNE
                     PWLAALRKVRERGIFVGMLSNMPPAWDVHWRAMVPPEDLFDDVVLSFEAGLRKPVREIF
                     DLATSTSGIEPERSVFIDDLAVNCAGAQDAGWHSIHFQDTDEAIRALEALTTAPVTTS"
     gene            6807..7511
                     /locus_tag="TL08_RS23775"
                     /old_locus_tag="TL08_24105"
     CDS             6807..7511
                     /NRPS_PKS="Domain: PKS_KR (3-157). E-value: 4.4e-18. Score:
                     57.8. Matches aSDomain:
                     nrpspksdomains_TL08_RS23775_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567714.1"
                     /locus_tag="TL08_RS23775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24105"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_069852274.1"
                     /sec_met_domain="adh_short (E-value: 9e-31, bitscore:
                     105.5, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSVLVTGGNRGIGAAIATAFVEGGDKVAVTYRSGEPPEGVFGV
                     RCDVTDTASVEAAVDEVRSQQGPVEVLVANAGIARDALFLGMAEQEFADVIDTNLMGSV
                     RAVKAVVTDMVRARWGRIILVGSTTAFWGGAGVTNYASSKSALVGLARSLAWELGPRKI
                     TVNVVYPGLIETEMISSMSAKSREHVLANTPLKRVGTPQEVATAVRFLADDGAGYITGA
                     LVPVSGGNGMGH"
     CDS_motif       6816..6887
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TL08_RS23775_0001"
                     /evalue="1.00E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="TL08_RS23775"
                     /protein_end="27"
                     /protein_start="3"
                     /score="18.2"
                     /tool="antismash"
                     /translation="SVLVTGGNRGIGAAIATAFVEGGD"
     aSDomain        6816..7277
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TL08_RS23775_PKS_KR.1"
                     /evalue="4.40E-18"
                     /label="TL08_RS23775_PKS_KR.1"
                     /locus_tag="TL08_RS23775"
                     /protein_end="157"
                     /protein_start="3"
                     /score="57.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="SVLVTGGNRGIGAAIATAFVEGGDKVAVTYRSGEPPEGVFGVRCD
                     VTDTASVEAAVDEVRSQQGPVEVLVANAGIARDALFLGMAEQEFADVIDTNLMGSVRAV
                     KAVVTDMVRARWGRIILVGSTTAFWGGAGVTNYASSKSALVGLARSLAWE"
     CDS_motif       7173..7262
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TL08_RS23775_0002"
                     /evalue="1.30E-04"
                     /label="PKSI-KR_m4"
                     /locus_tag="TL08_RS23775"
                     /protein_end="152"
                     /protein_start="122"
                     /score="14.5"
                     /tool="antismash"
                     /translation="ILVGSTTAFWGGAGVTNYASSKSALVGLAR"
     gene            complement(7635..8951)
                     /locus_tag="TL08_RS29155"
                     /old_locus_tag="TL08_24110"
     CDS             complement(7635..8951)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024949.5"
                     /locus_tag="TL08_RS29155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24110"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_318533336.1"
                     /transl_table=11
                     /translation="MSPRGFDALLSVFGGDRVQHQTTTSLFALALRRTRESAGASLSAL
                     ARKAGYHPSTLSKIENGQRDASRDLAEDLDTALKAQGALLKVWHAARSTTDTDVSLIWV
                     LIPTSEGVSPMALSRRDLVAAILAAAGGAAMAAPPSALATTTNPSPETILGMRRLLEEF
                     WRLGRITDPATLIPSLTGQISTIGTFAPNATGSVRTDLFGLGARFAEYLAYMFEEIDDR
                     SAARYWIDRGEQMARAAADVEMAAYMWVRRSRVLASDRSTAEAIAAAQRARSASVGPSL
                     RTVSLLREAEGLAQRGEDTRAHRALDAAAEFHIVADPAEGTWCSRTVPGVVEVVRARVL
                     VDLGRPGDAAEVLDGQLPMIPATSRRQRMRYGLLLALARSREGEHEEAIRLTRVALDEG
                     MGVRSAEARALAVELRESLAWSDASGARALRRDLAALPG"
     gene            9127..9402
                     /gene="tgmA"
                     /locus_tag="TL08_RS23785"
                     /old_locus_tag="TL08_24115"
     CDS             9127..9402
                     /codon_start=1
                     /gene="tgmA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04186.1"
                     /locus_tag="TL08_RS23785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24115"
                     /product="putative ATP-grasp-modified RiPP"
                     /protein_id="WP_172803866.1"
                     /transl_table=11
                     /translation="MVTTLLPTGPFHFQISMDEPSAARPFGLTKATPIATEDMTRLAEV
                     VYDDERQISVTRDGTPLVDHPLSAVTTSSRLTIQDMQEWPDYDPDD"
     gene            9395..10378
                     /gene="tgmB"
                     /locus_tag="TL08_RS23790"
                     /old_locus_tag="TL08_24120"
     CDS             9395..10378
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tgmB"
                     /inference="COORDINATES: protein motif:HMM:TIGR04187.1"
                     /locus_tag="TL08_RS23790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_24120"
                     /product="ATP-grasp ribosomal peptide maturase"
                     /protein_id="WP_069852278.1"
                     /transl_table=11
                     /translation="MTEPNVDRHVLVITKDFDPTADDVVTRLTDLRVPVMRFDLADFPI
                     QLAVSGCIGPGARRWSGRIRGSHRSVGVEDIGAVWYRKPSRFRTLAGMSGTEETWAIGE
                     ARAGIGGLLGTLRCRWINHPVRNVIGSDKPMQLVVADEVGLSIPETLITNDPELARSFV
                     AAQPGGAIYKSFRGGPRSESGRPIALFTTSVTSADITDAVRHTAHLFQACLPKAYEVRL
                     TAVGNTMFGLRIDVETANGRQDWRSDHRRLTYSTIQIPDTIARGVRRLMHRLDLMFGAL
                     DFVVTPTNEWIFLEINPNGQWAWPHPQREAIADALATALEEGVRAE"
ORIGIN
        1 acacaattga cccgctgccg gatcgcgagc acccagtacc gaggtttctg atgggggtca
       61 gatgagtaac tctacctacg cgttaccgcc ggcggccagt atccaaccgc cgcctgccaa
      121 ccgagacatg caacaacgta tcgatattgt cgacgagttc gagttcagac tcggggtaag
      181 cgagatctcg cgtacgaccg accccggctt ctggccgcag gttcgggcgc aggtctccat
      241 gattctcgac gactgcctgg ccaagatctc cgatcccgac ttcgtgccga gtcaggagac
      301 cagggccgcc gcgaacgact tcggctccga ggtggccaag cgcagcgttc ggcccaacga
      361 tgtcctcgac gcgggctcgg tgctcttcga gctcgtctcg gagagcttcc tgcaggacgc
      421 cgggcccacg gccgccccgc gcaggctcgt cgagatgctg cggatcctgg aacggacgat
      481 ctccgagcac gtgacggcga tcctcgacgg ctacgaacgc aacctgctcg aacgcgcggc
      541 gcactccaag gccgaggact ggcgaaggct cgccagggac atccacgact gggtcggcaa
      601 cagcgtcagc ctggtcgccc gcaacctcga cctctatcag ttggagcagt ccaggggcga
      661 cgagccgagc accgatcgga tcgtccgagc cagggcggcg gtcgacgacg tgctggaggc
      721 gacgcggcga gtgatctccg acctgcgtca ccaggagccc gcgacctgta tcagtcacgc
      781 gctcagccgg ttcgtcgaag cggccaagcc cgagcgcacc cgatcgcaca tcgtcgttcg
      841 gggcgaggag gcgcaattgc cgctggatca tcgcgatgcc ctcttcgtga tgctccgcga
      901 gtgcctgcgc aacaccattc gtcatgcgga cgccgagaac gtggcggtct ccgtcgacat
      961 cgaacccaag tcggtccggg ccatcgtcat cgacgacggt gtcggtttcg acgtcgacgc
     1021 ggtgcatcgg gctggcctgg cctacgggct ggactcgatg cgcgagcggg ccgcgctcct
     1081 cgggggcaag gcgaccatca ccagcgcccc cgacagcggc accacggtcg agatctcgtt
     1141 gccgctagga gtcgtcgatg gctgccgcaa ctgacaccat ccgcatcctg ctcgtcgacg
     1201 accacaccct gctccgggag gcggtccgcg acgtcctgct caccgaggac gacttcaccg
     1261 tcgtcggcga ggcaggcgat ggcgcgaccg cggtggggtt ggccgcgacg acgaagcccc
     1321 acgtggtgct gctggatgtc gagatgccgc gcagcaaacc gctcgagacc gtcgaacgcc
     1381 tactgcaggt ggccccgaaa gccagggtga tcgtgctgag catgtacgac gatccccagt
     1441 tggtccgcga gatgctgggc ctgggcatcg caggctacct gcacaagagc atcggccagc
     1501 gcgatctgat caacgcgatc cgcagcgccg gaaccggcga ggggcaggtc accgtgtcgg
     1561 tgtcccggga ggcgctggcg ctgtccgggg ccaacgccga ggtgtccggt ccgctgtcga
     1621 gccgagagat cgaggtgctg gccctggtgt cggaggcgat gagcaaccga cagatcgcgg
     1681 gcaagctctc catcacggag ggcaccgtga aacgccatct acgcaacatc ttcgccaagc
     1741 tcggtgcgat gtcccgtatc gacgcggtga acaaggccgt ggatctcgcc ctgatccgca
     1801 aacggtactg accaccagcc ccccatcggt ctggtgcatc gatcgccagc cggcgtcgcg
     1861 cccccccggc gccgccggtg cttccccgaa caggatcgcc gggtgtccgc tcccccgatt
     1921 cctgatctcg cacaccattc ctcgggcacc ctggacaacg tcggtccacg gcaggcccct
     1981 cgcgccgaac tcgagatccg gtgccgcccg tcggaagccg gtcggcgggc cgtcgggcgc
     2041 atgcacgccg acgacccgcc gcagactccc cggttcaggc ggcctcgcga atcaccgcgc
     2101 agcccaggtt cccgtccggt cccaccgagg tcacgacacc gaccctcggc cgctcggcgg
     2161 gttcccgccg ccacagggcc agcagcgcgg cgacctggag cgccccgctg gcgctgaaac
     2221 actcgccgac gacgtcggcc acccggatcc gctcggctcg ttcgccgagc acctcggtga
     2281 ccgcccgttc ctccacccgc cgcagcgtgg gactgcccac cgcacccaac gacacgacgt
     2341 ccacctcgtc gggagtcagg ccgctgcggg tcaaggcccg gtcgagcagc gtgcgaagca
     2401 ctcgggtggg gatggttctg ctgcccggag ccgcgaaacc gacctcgcag gccagtagtt
     2461 gggcgatcgc ggagtcccgg tcctgttcga cctcggacac catcagcagg gcagcgccct
     2521 cgcccaacgg cacgtccgag ggcaccaccg aggtccgatg ccagccccag gcgagttgcg
     2581 ggctgagttc ctccacgcca ccgacgagca gccgaccggc gcgtttccta ccgatggcga
     2641 ccctggcgta ccgcagggcg tgcaggctgg acagctggcc tgcggccaac gtggcgttga
     2701 ccccctgcag cgagttgcgg atggcgatct gccccgcaca gctgttcatc accgtgttcg
     2761 ggaaccgcga ggggttgacc aggtacggct tctcctcgat cagggtgtcg cgcccgatct
     2821 cggcctgggt gcggagactg cccgccgcgg tgccgatggc gacgccgacg tcgggctggt
     2881 cgtcctcggc caccgggccg gtctcgtcga gcagctgctt ggcggtgatc agcccgagca
     2941 gcgtcaaccg gtccaggttc cgggttcccc ggcggccgag gaactcggcc acccgcaagt
     3001 cggcgatggt gcgcaccggc cgaggtggga agtcctcctc ggtgctccgc tccgccgagg
     3061 tgccctccac agcggacgcg gtggcgcgat ccgctgtacg cagtcgatcg ccgagcacct
     3121 cgatgccgaa tcccgcggcg ctgaccgccg agcaggcggt gatcgtcaac ggttccaccg
     3181 tgctcgcggc ggtcatcaca gcctcccgaa gatggtgatg gcgttgttgc cgccgaaggc
     3241 gaacccgtgg ttctgcgcga tgcgcaccgg ttcctccctg gccaccccgg gcagcgggtc
     3301 gattccccta cccagcgccg ggtcgatccg ctgcaggttc gcggtcggcg gcaggaaacc
     3361 ctcgtgcacg gcgaggcagc agatcagcgc gccgaagccg ctggcggctc ccatcgtgtg
     3421 gccgatgctg gatttgatcg agctgatcgg cggcagcgtc tcgccgaaca ccttgcgggc
     3481 ggcgctgacc tcggtgctgt cgttggtcgc cgttccggtg ccgtgcgcac agatgtagtc
     3541 gatgtcctcc ggcgtgacac cggccgagtc gtgggcgagc cggatgcact cggcgatccg
     3601 ctccgaatcc gggttcacca tgtgccgggc gtcgcagttc atcccgtaac cgaggacctc
     3661 ggcgtagatg cgagcacccc tggcctgggc gtgctccaac ggttccaaga gtagggccac
     3721 gccgccctcg ccggtgatga tcccggagcg gtcgacgtcg aacggcctgc acacgccgtc
     3781 ggtgctcaac gccccgaggc gatagaaccc ggcgtgggtg gaccggttca ccgcatcggc
     3841 gccgcccgcg atcatgaagt cggcctcgcc ggtgcgcacc aggtcgtagg cgtagccgat
     3901 ggcgaagttg ctcgccgagc acgccgttcc gagggtgacc gcatcgccgg tgagtccgag
     3961 ctcgctgttg acggcattgg cgatctggct ggccaaggcc tgcggaacga tccggccgtc
     4021 gatgctcttg agcccgtcgg ccacccattg gccgaccaac tgcccgatcg gcaccgattc
     4081 gccgccggtg gtgccgatga tggagcctgc ggccgaggcg gccagctcgt cgggatccag
     4141 gccggagtcg ccgacggcct ggcgggcggc ggccgcggcc aacaggctgg accgccccca
     4201 ttgctcgacg tcgagattgc ggatgagcgc cgtgggatcg aagtcgggca cctcggcggc
     4261 gatcgtggcg gggaatcccg tgctgtcgaa ggaattgatc ggtctgaagc cgttgcgccc
     4321 ggcgcggatg cccgccgcga agacatcggc tccggtcccg atgctgctca cggcgccgag
     4381 gccggtgacg acgactcgac gcggcatcgg ctcaggcatt gtcggcctgt ccgatcccgt
     4441 agcggtcgac ggccgagaag accaggccga gggtgctcag attcggcagc tcgtcgcggg
     4501 gaatgaccac cccgagttcc ttctcgatcc tggcgaccac ggtgatcagt gacagcgagt
     4561 cggcatcgaa gtcgtccacc agatgccccg actcggtgag ctcggacggg tccagctcta
     4621 attcgttggc gataatctcg cggatcttgt cgcgtactcc gtcagcgatt ccctgcgtca
     4681 tttcctgatt cctgtctctc gattcgagcg attccgatgg cactcctgcc agcgacgatc
     4741 ggcggggtcg acggccatcg gcgcccgtca aaccgcatcc tcccggcgat ctgggatcga
     4801 tgctacgaat gtttcaacca cctcgacaag tggtttcaaa gtggcacgtc gaattattgt
     4861 tcgggctctg accaggcttt gatcgctggc tgaacgggct ttgatcagac tattcaagaa
     4921 tggtgttcca ccacatcgaa tcgccgacac ccgtgatgtc tcccatcccg gagcagggcg
     4981 cgaaaggagc cggaaccgcg atgacgacgc ctgtcttgtc caccagcacg gcgccgcccg
     5041 gtctcgatgc cgccgaactc agcttcacca agaccatcga tcggggtctg gtgcaccgga
     5101 gttcggtgtc cgaggtgttc gtgaccgacc tacaccaggc cggtgagttg gccttcgccg
     5161 ccgccgcgca actaccgttg acgcactcct actacaacga ccacgtcgcc cggcctgcga
     5221 tggtggatcc gctgctcatc gtggagtgcg cgcgccaagc ggggatctac ggggcgcatc
     5281 tgtccggggt gcccatgcgg accgcgatgt tggtgaacag cttcaccctg cggctggacg
     5341 atccgaccga gttgatcatg tcccggcggc cggtcgaact acggatcgac agtgtcatgg
     5401 ccgccacccg ggtgcgcggc ggcgtgctgc gccagggaca cgccgagcag cggttcacca
     5461 tcggcgggcg cccggtcggc agccaggtga tggagatcca gatgctctcc caccacgaac
     5521 acgacgccct gcggcatctg caacggggca atcccgcgcc gagcacggcg gacatgaccg
     5581 gcggccccta tccgaattcg gtggcgccgg gtctggtcgg ccgggtgcat ccgatgaacg
     5641 tcgtgctcgc cgacgtccga tacggcgccg acgaggttcg ggcggtgatg gcgccctggt
     5701 acgagaaccg ggcgctgttc gaccacgact acgaccacct gcccgccatg accctcaccg
     5761 agggcgcgcg ccagctggcg ctgctgtcgg tccgcgaatc gggagcgacc gaggcggccg
     5821 gattcccgcc ggtggtgggg atcgccgcga cgttccaccg attcgccgaa ctcgacgcac
     5881 cggtccacgc gacgaccgcc accgagcccg cagccgaggc aggcgcgctg accaggacgg
     5941 tcgtcttcac ccaggacggg atcacgatcg ccacgatctc gatctcgctc gccatgcccg
     6001 cgtcgatcgg agaacgacga tgaccgagcc cacccgtacc gtcgacgcag tctggaccga
     6061 tttcggcggc gtgctgaccc cgccggtatc cgagacgatg gctgccttct gccgcaagaa
     6121 gaatctggac cccgccccgt tgggtcgggc catctggaag gtcacccaga gctacggcac
     6181 caccgacatc atggagccgc tggacacccc gctggtcagc gaacgggact ggctccgcca
     6241 ggtgaccgac gcgatgttcg aggacacggg catccgcgtc gagatcacct cgatgggcga
     6301 ggactggttc gacggccgag ccgtcaacga gccgtggttg gcagcgctgc gcaaggttcg
     6361 ggagcgcggg atcttcgtcg ggatgctctc caacatgccg cccgcctggg atgtccattg
     6421 gcgagcgatg gtcccgcccg aggacctgtt cgacgatgtc gtgctgtcct tcgaggcagg
     6481 gctgcgcaag ccggtccggg agatcttcga tctcgccacc tcgacctccg gcatcgaacc
     6541 cgagcgcagc gtcttcatcg acgacctggc cgtgaactgc gcgggcgcgc aggacgcggg
     6601 ctggcattcg atccacttcc aggacaccga cgaggccatc cgtgcactgg aggcgctgac
     6661 gacggcgccg gtcaccacgt cgtgacatcg gacagcacgc caggagccat gcccgcgcca
     6721 cgcaggcagg cggaccgcac ggcgaaagca ggcgcttccg ggcgaagccg ggcgccggag
     6781 aacccgaacc gagagaggag cacgaggtga gccgttcggt actggtcacc ggaggcaatc
     6841 gaggcatcgg cgcggcgatc gcgacggcct tcgtcgaggg cggtgacaag gtcgcggtca
     6901 cctaccgctc cggggaacca ccggagggtg tgttcggtgt tcgctgcgat gtcaccgaca
     6961 cggcctccgt cgaggccgcg gtcgacgagg tccgcagcca gcagggcccg gtcgaggtgt
     7021 tggtggccaa tgcgggcatc gccagggacg ccctgttcct cggaatggcg gaacaggagt
     7081 tcgccgacgt catcgacacc aacctgatgg gttcggtgcg cgcggtcaag gccgtcgtca
     7141 ccgacatggt ccgcgcccgg tggggccgga tcatcctggt cggatccacc accgcgttct
     7201 ggggcggcgc aggcgtgacg aactacgcct cgtcgaagtc cgccctggtg ggactggccc
     7261 gctcgctggc ctgggaacta ggtccgcgca agatcaccgt gaacgtcgtc taccccggac
     7321 tcatcgagac cgagatgatc agctcgatga gcgccaagag ccgtgagcac gtgctggcga
     7381 acacgccgtt gaaacgtgtc ggcaccccgc aggaggtggc gaccgcagtc cgattcctcg
     7441 ccgatgacgg cgccgggtac atcaccggcg ccctcgtgcc ggtcagcggc gggaatggga
     7501 tggggcactg accccgcagg gcggaggcgg cttaccccag aggccgcctc cgtccgtctt
     7561 ttatgagcgc catggcctgc cggtggcatg ttggtgagtg tgctgtccgc tgtctccgtg
     7621 ccgaccgctg cgactcagcc gggcagcgcc gccaagtccc gacgcaaggc ccgtgcgccc
     7681 gatgcgtccg accaagccag cgattcgcgc aactcgaccg ccagcgcacg cgcctccgcc
     7741 gaccggactc ccataccttc gtccagtgct acccgagtga gcctgatggc ttcctcgtgc
     7801 tcgccctcgc gcgaacgagc gagagcgagc aacaacccgt agcgcatccg ctggcgcctc
     7861 gacgtcgcgg gaatcatcgg caactgtcca tcgaggacct ctgcggcgtc accgggcctg
     7921 ccgagatcga cgagcacgcg cgcacgcacc acttcgacga cgcctggcac cgtccggctg
     7981 caccaagtgc cttcggccgg gtcagccacg atgtggaatt ccgctgcggc gtccaaggct
     8041 cgatgtgccc gagtgtcctc gccacgttga gccaaccctt ccgcttctcg caggagcgaa
     8101 acagtccgca acgacggtcc gacgcttgca ctgcgtgctc gttgcgcagc agcgatcgct
     8161 tccgctgtgc tgcgatccga ggcgagcact cgggatcgcc gaacccacat gtacgcagcc
     8221 atctcgacgt cagcagccgc tcgagccatc tgctcaccac ggtcaatcca gtaccgagcc
     8281 gccgagcgat cgtcgatttc ctcgaacatg tacgccagat attcggcaaa gcgtgcgccg
     8341 agaccgaaga gatccgtacg aaccgaaccg gtcgcgttcg gagcaaacgt gccgatggtc
     8401 gaaatctgcc cggtcaggct cgggatcaag gtggctgggt cggtgatcct gccgagccgc
     8461 cagaattcct ccaacagtct ccgcatcccc aggatcgtct ccggcgaggg attcgtcgtc
     8521 gtggccagtg ccgatggtgg cgcggccatc gccgccccgc ccgcggccgc aagaattgcg
     8581 gcaacgagat cacgacgaga cagggccatc gggcttactc cctcgctggt tgggatcaga
     8641 acccaaatca ggctgacgtc agtatcggtc gtcgaccgtg ccgcatgcca gaccttgagc
     8701 aaagcgcctt gagccttcag cgcggtatcc aggtcctcgg ccaggtcccg gctggcgtct
     8761 cgttgcccgt tctcgatctt ggacaacgtt gacgggtgat acccagcttt tcgtgccaac
     8821 gcgctgagcg aggcaccagc ggactccctt gttcgccgca gtgccagtgc aaacagactg
     8881 gttgtggtct ggtgctgcac acgatcaccc ccgaacacgg agagtagtgc atcgaatccc
     8941 ctcggcgaca catcgccgac cagcgctgcc aacgcttgtc aacgctcgct tgttccgcat
     9001 agccaaccta acggtgatcc tagatatctg acaggcgatt tcagccctcg atgaagcgat
     9061 cccgacttct cccgatgaac aaggagaagc gcctctgaag gaaggtgaag caatatggac
     9121 gatgcattgg tcaccaccct gcttcctacg ggaccattcc acttccagat ctcgatggat
     9181 gaaccttccg cagcacggcc gttcggcctc acgaaggcca ccccgatcgc gacggaagac
     9241 atgacgcggc tcgcagaagt cgtctacgac gacgagcgtc agatcagcgt gactcgagac
     9301 ggcaccccac tcgtcgatca cccgttgtcc gcggtgacga ccagttcgcg gttgacaatc
     9361 caggacatgc aggaatggcc ggactacgac cccgatgact gagcccaatg tggaccgtca
     9421 cgttctagtg atcaccaagg atttcgaccc tactgcggac gatgtggtca cgcggctcac
     9481 tgacctccgg gttcccgtga tgcgtttcga cctggccgac ttcccgatcc agcttgcagt
     9541 gtccggctgt atcgggcccg gtgctcggcg gtggtcgggc cggatccgag gttcgcaccg
     9601 cagtgtcgga gtcgaggaca tcggcgcggt ctggtaccgc aaaccatctc gattccggac
     9661 tctggccggg atgagcggta cggaggaaac ctgggcgatc ggtgaggcgc gggcgggaat
     9721 tggagggcta ctcggaacat tgcgttgtcg ctggatcaat catccggtgc gcaacgtgat
     9781 cggctctgac aagccgatgc agttggtcgt cgccgacgag gtcgggctat cgattccgga
     9841 aacgctgata accaatgatc ccgagctggc tcgctcgttc gtagccgcgc agccgggtgg
     9901 tgccatctac aaatcgttcc ggggcggccc gcgctctgag tccggccgtc ccattgcgct
     9961 gttcacgacc tccgtgacct ccgcagatat caccgatgcc gtgcggcata ccgcccatct
    10021 gttccaggca tgcttgccca aggcatacga ggtacggctc actgccgtgg gaaacaccat
    10081 gttcggcctc cgaatcgacg tcgaaaccgc caatggccgc caggactggc gttccgatca
    10141 ccgacgtctg acctactcga ctatccagat cccggacacg atcgctcgag gtgtacgccg
    10201 gttgatgcac cgactcgatc tgatgttcgg agcgctcgat ttcgtggtga ctcccactaa
    10261 cgagtggatc tttctggaga tcaatcccaa cggccaatgg gcttggccgc acccgcaacg
    10321 agaagccatc gcagatgcct tggccaccgc actcgaagaa ggagttcgcg ccgaatgacg
    10381 gctcccaacg ctctcgagga tccttcggcc cttcgacagc aattcatcac tggtctgatc
    10441 gagtccgggg cgattccgaa cccgcagtgg caagaggcgt ttcgtaccgt cgcccgtcat
    10501 cgcttcgtca ctcgcttcac ggcgagcggt acccggactg cgttcgatct cgctgacccg
    10561 acacaacggt ccgcagcgct caaggcttgc tactccaatg agactctgct gacccaacag
    10621 gacgcaggtg gcagttacac cagctcatcg accgaggtca cactcatggc tttgatgctc
    10681 gatcagctcg atgcccgtcc gggacattcg gtcttggaga tcggtacggg caccggctac
    10741 aacgccgcac tactctgcca tgttctgggc aatgcggcgg tggcttcggt cgacatcgac
    10801 ccgaccttga tcgacgcggc tcgtcgcgca ctcgagtcga ctggataccg gccgacggtc
    10861 gtctgtgatg acggcgcaat gggaataccg gcgcatgcgc cgttcgatcg gataatcgcc
    10921 acctgcggtg ttctgcggat tccgcccgct tggccggctc agctgaaacc caacggagtc
    10981 atcgtggtga acgttggcat cggcttggcg cgacttgtcc gtg
//
