LOCUS       NZ_CP014859            10855 bp    DNA     circular CON 20-NOV-2024
DEFINITION  Actinoalloteichus hymeniacidonis strain HPA177 chromosome, complete
            genome.
ACCESSION   NZ_CP014859
VERSION     NZ_CP014859.1
KEYWORDS    RefSeq.
SOURCE      Actinoalloteichus hymeniacidonis
  ORGANISM  Actinoalloteichus hymeniacidonis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Actinoalloteichus.
REFERENCE   1  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J.,
            Zotchev,S.B. and Ruckert,C.
  TITLE     Complete genome sequence of the actinomycete Actinoalloteichus
            hymeniacidonis type strain HPA 177T isolated from a marine sponge
  JOURNAL   Stand Genomic Sci 11, 91 (2016)
   PUBMED   28031775
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J., Zotchev,S.
            and Ruckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 159x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001747425.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 01:17:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,434
            CDSs (total)                      :: 5,374
            Genes (coding)                    :: 5,337
            CDSs (with protein)               :: 5,337
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 3 of 37
            Pseudo Genes (incomplete)         :: 34 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:46:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4689563
            Orig. end                         :: 4700418
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10855
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="22"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10855
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10855
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5855](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5855)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            875..1378
                     /locus_tag="TL08_RS19540"
                     /old_locus_tag="TL08_19805"
     CDS             875..1378
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TL08_RS19540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TL08_19805"
                     /product="hypothetical protein"
                     /protein_id="WP_069851036.1"
                     /transl_table=11
                     /translation="MTDATLTELISRAYGGDLTRPDLARPRRRLRANPYADLIDEVSHL
                     DGVLLTDYTDSNHMVCLGYLLAHGGQEWALELSLIAPFAIFARTSDVWERLLHPSTTDL
                     DTTEQQVIALLTSHGFSLPHRETLEQPLAMSLDYTDPENVRVYHALFSDEDFLPWEFSH
                     HYRE"
     gene            complement(1404..2927)
                     /locus_tag="TL08_RS19545"
                     /old_locus_tag="TL08_19810"
     CDS             complement(1404..2927)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.5"
                     /locus_tag="TL08_RS19545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19810"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_084643272.1"
                     /transl_table=11
                     /translation="MDVNAHPEVLVIGAGPSGLLAACELLRRGIGVRLVDAATAPVSYS
                     KALLLWPRTVDVLENLGAGPAVEKHSVRINSFRYYSAKRQLADVRFPPELAARALPQRY
                     TEDILRERLTALGGTIEYGVRLLALDRMDFSGDPAATDGVTAVLEHADGRVERCTADWL
                     IGADGAGSAVRAQLGLAFTGSTYASRFVLGDAHIEGGLADDEAHYFQSPAGVLVVVALP
                     DGLFRFFASAPPEQQNGRVDLPLIQRIVDERGPGGIRLVDPQWLSGFRVHRRQADRFQL
                     GRVFLVGDAAHVHSPAGGQGLNTGLQDGHNLAWKLASVIHDEAPMSLLTSYGPERHTVA
                     AQVVRDTDIQTQAWMARNPAIVAGRDLLFRAADRVGLIERGYLPIMAGRRWRYRPADSV
                     ALLAPQPNEDRGALRVGTAAGPILLSPKDGQRDLRWTLLTEGVSCPPPAPSVRVLPAQG
                     RWANLSGCARTAFVLVRPDGVIAARGRSAELTRACRWVADLLEVPQTPM"
     misc_feature    complement(2550..2552)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3117..4130)
                     /locus_tag="TL08_RS19550"
                     /old_locus_tag="TL08_19815"
     CDS             complement(3117..4130)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076296.1"
                     /locus_tag="TL08_RS19550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19815"
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="WP_236750339.1"
                     /sec_met_domain="fabH (E-value: 5.7e-89, bitscore: 296.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMTLPTNTLPDRLTTEVGTLGWGCYLPDRLVTNDEVARPAGVDA
                     SWIERKTGIRLRRRAASHEAASDLATDAARHALAQAGVRAEDLTAIVLATSTPDHPQPP
                     TACLVQERLGATSAAAFDLNAVCSGFVFALGVARDMLAATGGLALVIGVDVYSRILDPA
                     DRKTAILFGDGAGAAILGPVPGGRGVQAIRLLSTGSENQLIRVPAGGSRIPATEESVRA
                     GQHWFTMDGRGVRDFVEERVPAALTSFLTDSGVAPGEVRHLVTHQANGRMIDELVPRLG
                     LPAAEVHQSVQRYGNTGAASIPITLAEAADSIEPGDVVLLAGFGGGMAMGFALLRW"
     gene            complement(4127..4321)
                     /locus_tag="TL08_RS19555"
                     /old_locus_tag="TL08_19820"
     CDS             complement(4127..4321)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TL08_RS19555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TL08_19820"
                     /product="hypothetical protein"
                     /protein_id="WP_069851040.1"
                     /transl_table=11
                     /translation="MAAGYCTCGCAVAAWSLGAPSVPTAREVGLTVADLSGRPPSPRIH
                     NPGLDPSAFDELDELEDHR"
     gene            complement(4321..5004)
                     /locus_tag="TL08_RS19560"
                     /old_locus_tag="TL08_19825"
     CDS             complement(4321..5004)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="TL08_RS19560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19825"
                     /product="HAD family phosphatase"
                     /protein_id="WP_069851042.1"
                     /transl_table=11
                     /translation="MSGSARVAAVFDVDETVLSVKSMVDFLRFYLDETEIPAGSKQAGL
                     LAEFQAALTDDREPREQLNRRYYRVYTGERADRLRELGARWFAQRRAAPGFVNARIVAT
                     LLRHRLAGHLVVAVSGSFGACLEPLRAALGIDHVLCTEPIVDVDGRHTGEIVRSLIGDG
                     KPAALRRLAAAENIALPLSFGYADHISDVPLLELVGHPVAVHPDARLAAHAQRLGWARI
                     SPAPR"
     gene            complement(5001..5855)
                     /locus_tag="TL08_RS19565"
                     /old_locus_tag="TL08_19830"
     CDS             complement(5001..5855)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.5"
                     /locus_tag="TL08_RS19565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19830"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_069851044.1"
                     /sec_met_domain="AfsA (E-value: 4.7e-12, bitscore: 44.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESTPEMTRDSHLVAEGMSDRRAACVVGDRFIALTEIEEVQTVS
                     QFVAALRRGEPVPERVLAVQGVSQFELDYLREELNKRQAAGTEIVPPVSPRLGRGIVHK
                     QREPNVLLADLTATGPASYTAGLWLHVDNELFGDHQTGQHVQGIVIIEALRQMFIAVFE
                     MEYGRRHQDRDFYVVWDSIDITFLSFLFPLPATIHCDIVEADLSDESRAGYRMRMRVLQ
                     SEVCAAEATVRFGGYDHDRIEAAEAKRARRAVHDFLRSRIAPAQPVAPAQPVAPAQPVG
                     VTS"
     misc_feature    complement(5394..5396)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6216..6230
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
     gene            complement(6422..7051)
                     /locus_tag="TL08_RS19570"
                     /old_locus_tag="TL08_19835"
     CDS             complement(6422..7051)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809093.1"
                     /locus_tag="TL08_RS19570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19835"
                     /product="LysE family translocator"
                     /protein_id="WP_069851046.1"
                     /transl_table=11
                     /translation="MISPAAIMGFALAVLPLIVTPGASLTLLAQRVATGGLRKGLPVIL
                     GTATGLYLHASLAILGLSALIIGSSRLFLVVRFLGAIYLIGLGVWTWRQATRPPNVSTS
                     MPATPSDRAPSTYVPAVLANVLNPKAASIPLSITPQFLDPDASVGGQILLFTTMHALLS
                     ACWLLLWTLILGRGAGLLHSARAKAMFNRAAGAMLLALGLRAAVGE"
     misc_feature    complement(6836..6838)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7048..7884)
                     /locus_tag="TL08_RS19575"
                     /old_locus_tag="TL08_19840"
     CDS             complement(7048..7884)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Metallophos_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671409.1"
                     /locus_tag="TL08_RS19575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19840"
                     /product="metallophosphoesterase"
                     /protein_id="WP_069851047.1"
                     /sec_met_domain="Metallophos_2 (E-value: 1.1e-07, bitscore:
                     30.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLLAQISDLHLDGTARAIERADRVMDHLRALPRAVDAVLVTGDI
                     ADRGDPAEYRQAAEILTCESPVLVCPGNHDERRAFRSGLLGKLGAPLGAAAESAARDTE
                     SAPINQLHRIGDAAIVLLDSTIPGSPDGLLTPDTLDWLTSTLDDLGGTVPVLLALHHPP
                     VDLHHPLPDRYPLRRREDLAEVLAGRPEVVAILGGHAHTAATSMFAARPVILAPAVTWT
                     LRMPWEGDSPVDLAAPPALAFHVLEDWRLTTHFRILASPASERGRVGSAGRRDQAP"
     misc_feature    complement(7855..7857)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7862..7875)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            8099..8713
                     /locus_tag="TL08_RS19580"
                     /old_locus_tag="TL08_19845"
     CDS             8099..8713
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809097.1"
                     /locus_tag="TL08_RS19580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19845"
                     /product="ABATE domain-containing protein"
                     /protein_id="WP_069851049.1"
                     /transl_table=11
                     /translation="MTGRQLAMQLAGTIRHDGAGGVADDLADPAGLTAWLADHAELLPE
                     VAGPGRIPADEQTRSAVVAVRSATRALFARTVPGRPSRADEHRLPPIADAVAILNAASA
                     ADPVAPALNWSSGSAPAVELHSASNDPLTRLLATLARAAFDFLTGPDRDRLRACTAPRC
                     VRYFLQGHGRQEFCKPACSNRARAARHYDRRHPERSNLSLG"
     misc_feature    8863..8878
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 23.65"
                     /tool="antismash"
     gene            8908..10401
                     /locus_tag="TL08_RS19585"
                     /old_locus_tag="TL08_19850"
     CDS             8908..10401
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463964.1"
                     /locus_tag="TL08_RS19585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_19850"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_236750340.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5.2e-24, bitscore:
                     83.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEDHDGQSAPTAHRQAGSTSIPQAAGRPGSRYVIKGGAVMSMDP
                     TVGNFENADVLIEGKRIVAVGPGIDASDAEVIDARGRIVMPGFVDTHHHLFETAERALL
                     ANGLLLDDGSGSPSSFPAYAQHILGGFAKAYRPQDVYINALFGRIAQLDAGVTTVLDVS
                     QIHHSPEHTDAAIQALVDAGGRSAFGFFEGDDRASVRYPEDAYRLKNQWFSSDDQLVSL
                     VMGGELYIGEESYVRSWEIARELGVKIAAHAVGSWGMRPLYDEIAQGSGKVRVGPDNLF
                     IHMTGMSDDTWRRFRDAGAQVSISFPIEMVMRHGTPPILKMQELGMEPSLSSDVETTMT
                     ADPFTLMRSAMSMQRMAVNQMVLDQGDYVPPTNYPTPAEGTPELLTVRDVLRYATVNGA
                     QHLGLDGRTGTLTPGKDADIVLLDATALNVAPLNSVPGAVVTLMDRSNVETVIVAGEIR
                     KWQGRMLDANLTTLRQELETSRDFLFEAAGFDQDLFKYS"
     misc_feature    9229..9231
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10613..10627)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    10768..10777
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
ORIGIN
        1 atcttggagc tggccgaccc catgacagcg cttgatttac gcaatcacag tgcgcatgtt
       61 cccggcgtcc tcgggcagac gttcacgggt actgcgtccg aaacggcgct tgcagcgcag
      121 actcgggcaa cgttggcaga ccaggagatc ctggtgtgta ccgaggcact tcagaatggt
      181 caacgcgcct tgcttgccta cgtggagatg ctgtgatggg ggcatcgacc gaggatgtcg
      241 cccaaaccct catcagggtt cgacaagaac tgcctgttgc gcccgcgttg gatctagcgc
      301 acacggtcga cgaggcagta cgtcgcgccc agaatctggc agttgaatca accaactccg
      361 ctctgaaaga agctgtcatc cacttcgtga gcgcccgcga tgctcttcgc cgagccgcag
      421 gccactgggg cgcagtcgat gcaggagtaa ccgagtacct aatcgcctca ctcggagtcg
      481 aaggttcgat gaccacggct gtgcctacat cgggctactc cgatcccgag gcgctttcct
      541 ccctcgagga gcggcagcaa ccgaggggag tcagtaagca gaagcaggcg cgccatgttc
      601 tcgggacacg cgagtacgcc gagcggatac ggagcgagga tggcgggtcg gcgttctttg
      661 acgccgagtc ggcacatcga ctgaccatgc agacgtggtt ctcgggaaca ccttccaagc
      721 gcccctacgt ccgagaccgt gatttcggtg tgccggtcgg agtcagcagt tcgggcaggt
      781 accaaacacg tgtccgtgtc catctcgacc gcaagggtcg cctacacgga catcccagcg
      841 gccccgagat ctcccgagac agcgagccag aagtatgacc gatgccaccc tgaccgagtt
      901 gatcagcaga gcctacggcg gggacctcac ccgtcccgat ctcgcgcgtc cacgcaggcg
      961 cctgcgtgcg aatccctatg ccgacctgat cgacgaggtg tcgcacctcg atggcgttct
     1021 gttgaccgac tacaccgact ccaatcacat ggtgtgtctc ggctatctcc tcgcccacgg
     1081 cggccaggaa tgggcgctgg agctgtcgtt gatcgcacct tttgcgatct tcgccaggac
     1141 cagcgacgtc tgggaacgac tgctgcatcc gtcgaccacc gacctcgata cgacggagca
     1201 gcaggtcatc gctctcctca ccagccacgg attctcgttg ccgcatcgcg agacccttga
     1261 acaaccgctg gccatgtccc tcgactacac cgaccccgag aatgtccgcg tctaccacgc
     1321 gctcttctcc gacgaggact ttctgccctg ggagttctca caccattacc gagaatgagc
     1381 tctcccatcc gaatctcgct ggcctacatc ggcgtctgtg ggacctccaa caaatccgcc
     1441 acccaccgac acgccctcgt cagttcggcg gaccggcctc gtgcggcgat cacgccgtcc
     1501 gggcgcacca gcacgaaggc ggtccgcgcg cagcccgaca ggttcgccca cctaccttga
     1561 gcgggcagca cccggaccga gggggcaggc ggcgggcagg acacgccctc ggtcagcagg
     1621 gtccaccgca ggtcccgttg tccgtccttg ggcgacagca ggatcggacc ggctgcggtt
     1681 cctacccgta gtgcgccccg gtcctcgttg ggctgcggcg ccagcaatgc gaccgaatcc
     1741 gcaggccggt agcgccaccg cctgcccgcc atgatcggta gatagccgcg ttcgatgagg
     1801 cccactcggt ccgcggcccg gaacagcagg tcccggcccg ccacgatcgc tgggtttcgg
     1861 gccatccacg cctgggtctg gatgtcggta tcccgcacca cctgcgccgc aaccgtgtgc
     1921 cgttcggggc cgtagctggt caacagcgac atcggggcct cgtcgtggat caccgaggcg
     1981 agtttccacg cgaggttgtg gccgtcctgc agaccggtgt tgagtccctg gccgcccgcc
     2041 ggactgtgca cgtgggcggc atcgcccacc aggaagaccc ggcccagctg gaaccggtcg
     2101 gcctgcctgc ggtgcacccg aaagccgctg agccactgcg ggtcgacgag tcggatgccg
     2161 ccgggcccgc gttcgtcgac gatgcgttgg atcaggggca ggtccacccg gccgttctgc
     2221 tgctccggcg gtgcactggc gaagaaacgg aacagcccgt cgggcagggc caccaccacc
     2281 aacacgcctg cgggcgactg gaagtagtgc gcctcgtcgt cggcaaggcc gccctcgatg
     2341 tgagcgtcgc cgaggacgaa ccggctggca taggtggaac cggtgaaggc cagaccgagc
     2401 tgggctcgca ccgcactgcc cgctccgtcc gcgccgatca gccagtcggc cgtgcagcgt
     2461 tcgacccggc cgtcggcgtg ttccaacacg gcggtgacgc cgtcggtggc ggcgggatcg
     2521 ccggagaagt ccatccggtc cagggccagt aatcggacgc cgtattcgat ggtgccgccg
     2581 agcgcggtca gcctctcgcg caggatgtcc tcggtgtagc gctgcggcag cgctcgcgcg
     2641 gccagttccg ggggaaaccg cacgtcggcg agttgtcgtt tcgcggagta gtagcggaac
     2701 gagttgatcc ggacgctgtg tttctccacg gccgggcccg caccgaggtt ctcgaggacg
     2761 tcgacggtgc gtggccacaa caacaatgcc ttggaatagc tcaccggggc ggtggccgcg
     2821 tcgacgagcc gcacaccgat gccccggcgc agcagctcac aggcggcgag caggccgctg
     2881 gggcccgctc cgatcaccaa cacctcgggg tgtgcgttga cgtccatggc tttctctccc
     2941 tcgccagtgg agcttgttcg atgcccgaca tcggtcgacc cgatcggggt gtcctcattc
     3001 gtccgccgga acgggaaacc ggcggcactc gaaggcgccg gggcgttcga accacgctgt
     3061 tcgaacgcgc aggcgcccgg ttcgacgacg tgcgagtctc ggccgcaccg gctcgatcac
     3121 cagcgcaaca gcgcgaatcc catcgccatc ccgccgccga acccggcgag cagcaccacg
     3181 tcgcccggtt cgatggaatc ggcggcctcg gcgagggtga tcggaatgga ggcggctccg
     3241 gtattgccgt agcgctgcac actctggtgg acctcggctg cgggcaggcc gaggcgggga
     3301 acgagctcgt cgatcatccg accgttggcc tggtgggtca ccaggtgtcg cacctcgccg
     3361 ggtgccactc ccgagtcggt caggaaggag gtcaacgcgg cgggcacccg ttcctcgacg
     3421 aagtcgcgca cgcctcgacc gtccatggtg aaccagtgtt gaccggcgcg aacggattcc
     3481 tcggtcgccg gaatccgact gccgcccgcg ggcacccgga tcagttggtt ctcgctgccg
     3541 gtgctgagca ggcggatcgc ctgcacgcct cgaccaccgg gaaccggtcc gaggatcgcg
     3601 gcaccggccc cgtcgccgaa gaggatcgcg gtcttgcggt cggcggggtc caggatgcgc
     3661 gagtagacgt cgacaccgat gaccaacgcc agaccgccgg tggccgcgag catgtcccgc
     3721 gccaccccga gggcgaagac gaatccgctg cacacggcgt tgaggtcgaa ggcggccgcc
     3781 gaggtggcgc cgagtctctc ctgaaccagg caggcggtgg gcggctgtgg atggtccggg
     3841 gtggaggtgg ccaggacgat cgccgtgagg tcctccgccc gcacgcccgc ttgcgccagt
     3901 gcgtgccggg ccgcgtcggt ggccaggtcg gacgcggcct cgtgcgaggc ggcccgtctg
     3961 cgtagccgaa tgccggtctt gcgctcgatc cagctcgcgt ccacgcctgc ggggcgggcg
     4021 acctcgtcgt tggtcacgag tcggtcgggc agatagcagc cccagcccag cgtgccgacc
     4081 tcggtggtga gccggtccgg cagggtgttg gtcggcaacg tcatggtcat cggtggtcct
     4141 ccagctcatc gagttcatcg aaggcgcttg gatcgagccc ggggttgtgg atgcgaggcg
     4201 aaggcggccg cccgctcagg tcggcgacgg tgaggccgac ctcgcgcgca gtgggcaccg
     4261 atggcgcgcc cagcgaccac gcggcgacgg cacaaccgca ggtgcagtac ccggccgcca
     4321 tcaccgcggt gctggtgaga tccgggccca gccgagccgc tgagcatgtg cggcgaggcg
     4381 ggcgtccggg tggaccgcga cgggatgtcc gacgagttcg agcaacggca cgtcgctgat
     4441 gtgatcggca tagccgaagc tcaacggcag cgcgatgttc tccgccgccg ccagcctgcg
     4501 cagtgcggcg ggtttaccgt cgccgatgag gcttctgacg atctccccgg tgtggcggcc
     4561 gtcgacgtcg acgatcggtt cggtgcagag cacgtggtcg atgcccaggg cggcccgaag
     4621 cggttcgagg caggccccga acgatcccga tacggccacc accaggtgtc cggcgagtcg
     4681 atggcgcagc agggtggcca cgatccgggc gttgacgaag ccgggtgcgg cccggcgttg
     4741 cgcgaaccat ctggcgccga gctcgcgcag tcgatcggct cgttcgccgg tgtacacccg
     4801 gtagtagcgc cggttcagct gctcccgtgg ctcgcggtcg tccgtcagcg ccgcctggaa
     4861 ctccgcgagc agacccgcct gcttcgaacc cgcggggatc tcggtctcgt cgaggtagaa
     4921 ccgcaggaag tccaccatgc tcttgaccga cagcacggtc tcatcgacgt cgaacaccgc
     4981 ggccactcgg gcggatccgc tcatgaggtc acccccaccg gctgcgcggg ggcgaccggc
     5041 tgcgcggggg cgaccggttg ggccggggcg atccgactgc gcaggaagtc gtgcacggca
     5101 cggcgagccc gcttggcttc ggccgcctcg atccggtcgt ggtcgtagcc gccgaaccgc
     5161 acggtggcct cggccgcgca tacctcgctc tgcagcaccc gcatccgcat ccgatagccg
     5221 gccctggatt cgtcggagag gtcggcttcg acgatgtcgc agtgaatcgt ggcggggagt
     5281 gggaacagaa atgacaggaa ggtgatgtcg atcgaatccc agaccacgta gaaatcgcgg
     5341 tcctgatgcc gtcgtccgta ttccatctcg aagacggcga tgaacatctg tcgtaatgcc
     5401 tcgatgatga cgatgccttg aacgtgttgt ccggtctggt gatcgccgaa caactcgttg
     5461 tccacgtgca accacaggcc cgcggtgtag gaggcgggcc cggtcgcggt cagatcggcg
     5521 agcaggacgt tgggttcgcg ctgcttgtgc acgatgcccc ggcccaggcg aggcgagacc
     5581 ggcggcacga tctcggtgcc cgccgcctgc cgtttgttca actcttcgcg caggtagtcc
     5641 agctcgaact gcgagacgcc ctggacggct aggacgcgct ccggcacggg ttcgccgcgc
     5701 cgcagggcag cgacgaactg gctgaccgtc tggacttcct cgatctcggt gagtgcgatg
     5761 aatctgtcgc cgacaacgca ggctgctcgt ctatccgaca ttccctcggc gacgagatgg
     5821 gaatctcggg tcatctccgg tgtggattcc gccattgtct tctactcctt ggcacgtcac
     5881 atcgccggtt cacaatgtgg acgtgaaccg gtgataagcc gtctcgcgca cggctgaaca
     5941 cgagaaccgg tgaaccgcag ggcgcaccga cgatccagcg gtggattcgc ggcatagctc
     6001 ggacgtagtc ggtccgtcga tcagcagtcc tcgatgacgc ggattactcg cgtacctcga
     6061 agaccgcacc ccagtcggat cgggaggccg ccgcgtcctc gcgcacggcc accatcggtt
     6121 atggcagccc gatccgtcga aatgtgtagg cccgatcgtg atccgcccct gccgcacgat
     6181 cgatgcccac cgggcttcgt tgcccgttac gactcatcat gcccggcgat ccgataccga
     6241 caacagtgcc agattcggtc ggtcgtgatg cattcgcggc atagcccgct cgacgtagtc
     6301 gactgcgccg aatggcggac gcagcctacg tcgatgccgt ggtgccgtgg cggtcgcgtg
     6361 ctcgacgcac cgactgccac ggcgagcagt ggacgcgtcg aggaaacgtc ctcagggtcc
     6421 gctactcacc aaccgctgca cgtagtccca acgccagcaa catcgcgccc gctgcccgat
     6481 tgaacatcgc cttcgcacga gccgaatgga gcaggcccgc gccccggccg aggatgagag
     6541 tccacaacaa caaccagcag gccgacaaca gggcgtgcat ggtggtgaac agcaggatct
     6601 gcccgccgac cgaggcgtcg gggtcgagga actgcggcgt gatgctcagc gggatcgagg
     6661 ccgctttggg gttcagcaca ttggccaaca cggccggaac gtaggtgctt ggcgccctgt
     6721 cggacggtgt cgcaggcatc gacgtcgaga cattgggtgg tcgggttgcc tgtcgccagg
     6781 tccagacgcc cagcccgatc aggtagatcg cgccgaggaa tcgcaccacg aggaataagc
     6841 gactggagcc gatgatcagc gcggagaggc cgagtatcgc caaggacgca tgcaggtaca
     6901 gcccggtcgc ggttcccagg atcaccggca ggcctttgcg cagtccgccg gttgcgacgc
     6961 gttgagccag cagggtcagg ctcgcgcccg gcgtcacgat cagcggcagg acggccagcg
     7021 cgaaacccat gatggcggcg ggcgagatca cggcgcctgg tctcgtcggc cggcgctacc
     7081 gacccggccg cgctcggagg cgggcgaggc gaggattcgg aagtgggtgg tgaggcgcca
     7141 gtcctcgagc acgtggaagg cgagggcggg cggtgcagcc aggtccaccg gcgaatcccc
     7201 ttcccacggc atccgcagcg tccaggtcac cgcaggagcc aggatcaccg gcctagctgc
     7261 gaacatcgag gtggctgcgg tgtgtgcgtg accgccgagg atggccacca cctcgggtcg
     7321 gccagccagg acctcggcga gatcctctcg cctgcgcagc gggtacctgt ccggcagcgg
     7381 atggtgtaga tcgacgggcg gatgatgcag cgccagcagc acgggaaccg tgccgccgag
     7441 gtcgtccagg gtggaggtca accagtcgag cgtgtcggga gtcagcaggc cgtcgggact
     7501 accggggatg gtcgagtcca gcagcacgat cgccgcgtcg ccgatccggt ggagttggtt
     7561 gatcggcgcg gattcggtgt cgcgcgctgc ggattcggct gcggcgccga gtggagcgcc
     7621 gagcttcccg agcaggccgc tgcggaacgc ccgtcgctca tcgtggttgc ccggacatac
     7681 caggaccggg ctctcgcagg tcaggatctc tgcggcctgt cggtattcgg cgggatcacc
     7741 tcggtcggcg atgtcgccgg tgaccagtac cgcgtcgacg gcccggggca gggcgcgcag
     7801 atggtccatc acccgatccg cccgctcgat cgcacgagcc gtcccatcga gatgtaaatc
     7861 gctgatctgg gccaacagca ccatgtgtgc cgtcctctct gttccagtcg acctgcgaca
     7921 gacccgggcc gggttgtcga gaagggcacc acaccgaacc cagaggctaa cggtaaacta
     7981 cgagtattgc gttagtgatg ctggtcgagt ggtatccgcg agaatcactg gtcgcggccg
     8041 acaacgccga cccgatgcga agcccgcaaa cccgccacac cagaggggac ggcgatcgat
     8101 gacaggccga cagttggcga tgcaactcgc gggcaccatc cggcacgatg gcgcgggggg
     8161 agtcgcagac gacctggccg atccggccgg gctgaccgcg tggctcgccg accacgccga
     8221 actcctgccg gaagtcgcag gcccgggacg aatcccagcc gatgagcaga ccaggtctgc
     8281 tgtcgtcgcc gtgcgctccg cgacccgcgc gctgttcgcc cgcacggtcc cgggccgacc
     8341 cagtcgcgcc gacgagcatc gcctcccgcc catcgccgat gcggtggcca tcctcaatgc
     8401 ggcctcggct gccgatccgg tggcacccgc cctgaactgg tcttcgggtt cggctccggc
     8461 cgtcgagctg cactcggcga gcaacgatcc gctgactcgg ctgctcgcca cgttggccag
     8521 agccgcgttc gacttcctca ccggtccaga tcgcgatcga ctgcgcgcct gcaccgcccc
     8581 gcgatgcgtc cggtatttcc tgcaaggtca cggtcgtcag gagttctgca aacccgcctg
     8641 cagcaatagg gcccgagccg cgcgccacta cgaccgccgc catccagaac gatccaacct
     8701 ctcgctgggt tgattcggcc gccgagactg gtcggcgcgt tcgaatgacg cccgcccgtg
     8761 ccgccgctgt gcacgttcga ggttcggcaa cagcggcgtt cgaggacgac gcagtacctc
     8821 ggtccgccgg tacccgatag tcgtggaccc ggtaggcgtc cgacaccctt ggggagtaac
     8881 gaccatgccg gccgagacga ggttgatgtg accgaagacc acgatggaca gtccgctcct
     8941 accgcgcacc ggcaggcagg ctcgacgtct ataccgcagg cagccggacg gccggggagc
     9001 cggtacgtca tcaagggcgg cgccgtcatg tcgatggatc cgaccgtcgg caacttcgag
     9061 aatgccgatg tcctcatcga gggcaagcgg atcgtggcag tgggccccgg catcgacgca
     9121 tccgacgccg aggtgatcga tgcccgcggc cgcatcgtga tgccgggctt cgtcgacacc
     9181 caccatcatc ttttcgagac ggcggagcgc gcactgctgg ccaacggctt actcctggac
     9241 gatggctccg gctcgcccag ttccttcccg gcctatgcgc agcacatcct gggcggcttc
     9301 gccaaggcct accgaccgca ggacgtctac atcaacgcac tgttcggccg gatcgcccag
     9361 ctcgatgcgg gtgtcacgac agtcctggac gtctcacaga tccatcactc ccccgaacac
     9421 accgatgcgg cgatccaggc cctggtggac gcgggcggcc ggtcggcgtt cgggttcttc
     9481 gagggcgacg accgggcgtc ggtccggtat cccgaggacg cctaccgcct caagaaccag
     9541 tggttctcct ccgacgatca gctggtcagc ctggtgatgg gcggcgagct ctacatcggc
     9601 gaggagtcgt acgtccgttc ctgggagatc gcccgggagc tgggcgtcaa gatcgccgca
     9661 cacgccgtcg gctcctgggg aatgcggccg ctctacgatg agatcgccca gggaagcggc
     9721 aaggtccggg tcggaccgga caatctgttc atccacatga ccggcatgtc cgatgacacc
     9781 tggcgtcgat tccgcgacgc cggagcgcag gtctcgatct cgttcccgat cgagatggtc
     9841 atgcggcacg gcacgccgcc gatcctcaag atgcaggaac tcgggatgga gccctctctc
     9901 agctccgatg tcgagacgac gatgacggcg gacccgttca cgctcatgcg ctcggcgatg
     9961 agcatgcagc gcatggccgt caaccagatg gtgctggatc agggcgatta cgtaccgccc
    10021 accaactacc cgacccccgc cgagggcacg ccggaactcc tcaccgtccg ggacgtactg
    10081 cgttacgcga ccgtcaacgg cgcccagcac ctcggcctcg atggcaggac cggcacgctc
    10141 acgccgggta aggacgccga catcgtcctg ctcgacgcca ccgcgctcaa cgtggcgcca
    10201 ctgaacagtg tgcccggagc ggtcgtcacg ctgatggacc gcagcaacgt cgagacggtg
    10261 atcgtcgcgg gcgagatccg caaatggcag ggccgcatgc tcgacgcgaa cctgacgacg
    10321 ctgcgccagg aactcgagac atcgcgcgat ttcctgttcg aagcggccgg tttcgaccaa
    10381 gacctgttca agtacagctg agccgggatc ggggaggtgt cggcgcctcg acgcctcccc
    10441 gccgcccacg gatgcgcacc tgcctcagtg gtcgcgcgcc ccacggccga atcgttcgtg
    10501 cagcgccgcg tcgacctcgt cgaactcccg acccgaggtt tccgggacgt ggcgggcgac
    10561 gaaccagata ccgaaagcgc agatcgcggc gaagatccag aacgtctcgc cctggccgat
    10621 caggtccacc accggcagga aggccaggcc cacgatgaag ttcgccagcc agttcaccat
    10681 ggtcgacgcg ctcgacccga ccgcgcgcac cgagggcggg aacacctcgc cgatcagcac
    10741 ccagaacacc gggccgagtc cgcaggcgaa ggcggcgatg tagaccacca tgaagaccag
    10801 ggacagcagc ggcggcatct ccagcacgaa ggtgaggccc agcagtacca gcgag
//
